Aptamers serve as unique targeting ligands,making aptamer-drug conjugates(ApDCs)an attractive strategy for targeted cancer therapy.This study performs a comprehensive evaluation from rodents to non-human primates(NHP)...Aptamers serve as unique targeting ligands,making aptamer-drug conjugates(ApDCs)an attractive strategy for targeted cancer therapy.This study performs a comprehensive evaluation from rodents to non-human primates(NHP)of a protein tyrosine kinase 7(PTK7)-targeted ApDC(Sgc8c-M)made by conjugating the potent antimitotic agent monomethyl auristatin E(MMAE)to the classic PTK7 aptamer Sgc8c.Efficacy studies in various cancer types with PTK7 overexpression showed that Sgc8c-M effectively induces sustained tumor regression in cell line-derived and patient-derived xenografts,outperforming unconjugated MMAE,the chemotherapy drug paclitaxel,and a PTK7-targeted antibody-drug conjugate.Pharmacokinetic(PK)studies in mice revealed that Sgc8c-M leads to rapid accumulation and sustained MMAE levels in tumors,along with fast clearance from plasma and normal tissues.Further study in rats confirmed rapid clearance across most organs and revealed that over 75%of MMAE was excreted through urine and feces within 24 h.Toxicokinetic(TK)assessments indicated comparable systemic drug exposure without accumulation for repeated doses compared to single administration.Toxicity evaluations showed that the therapeutic dose with high efficacy was safe and that the toxicity resulting from extremely high doses could be reversibly controlled.Encouraged by these findings,we evaluated PK/TK profiles and safety of Sgc8c-M in cynomolgus monkeys.Similar to PK/TK profiles observed in rats,Sgc8c-M demonstrated good dose-dependent drug exposure.It was,moreover,well tolerated in monkeys with no obvious accumulation following multiple administrations.These findings highlight the potential of Sgc8c-M as an effective antitumor agent and provide useful insights for the clinical translation of emerging ApDCs.展开更多
基金funded by the National Key Research and Development Program of China(2023YFC3405100)the“Pioneer”and“Leading Goose”R&D Program of Zhejiang(2023SDYXS0001 and 2025C01109)+2 种基金the Zhejiang Provincial Natural Science Foundation of China(LDQ24B020002 and LDQ23B050001)the National Natural Science Foundation of China(No.22104132)the Zhejiang Province“Kunpeng Action”Program.
文摘Aptamers serve as unique targeting ligands,making aptamer-drug conjugates(ApDCs)an attractive strategy for targeted cancer therapy.This study performs a comprehensive evaluation from rodents to non-human primates(NHP)of a protein tyrosine kinase 7(PTK7)-targeted ApDC(Sgc8c-M)made by conjugating the potent antimitotic agent monomethyl auristatin E(MMAE)to the classic PTK7 aptamer Sgc8c.Efficacy studies in various cancer types with PTK7 overexpression showed that Sgc8c-M effectively induces sustained tumor regression in cell line-derived and patient-derived xenografts,outperforming unconjugated MMAE,the chemotherapy drug paclitaxel,and a PTK7-targeted antibody-drug conjugate.Pharmacokinetic(PK)studies in mice revealed that Sgc8c-M leads to rapid accumulation and sustained MMAE levels in tumors,along with fast clearance from plasma and normal tissues.Further study in rats confirmed rapid clearance across most organs and revealed that over 75%of MMAE was excreted through urine and feces within 24 h.Toxicokinetic(TK)assessments indicated comparable systemic drug exposure without accumulation for repeated doses compared to single administration.Toxicity evaluations showed that the therapeutic dose with high efficacy was safe and that the toxicity resulting from extremely high doses could be reversibly controlled.Encouraged by these findings,we evaluated PK/TK profiles and safety of Sgc8c-M in cynomolgus monkeys.Similar to PK/TK profiles observed in rats,Sgc8c-M demonstrated good dose-dependent drug exposure.It was,moreover,well tolerated in monkeys with no obvious accumulation following multiple administrations.These findings highlight the potential of Sgc8c-M as an effective antitumor agent and provide useful insights for the clinical translation of emerging ApDCs.
