In order to predict the lifetime of products appropriately with long lifetime and high reliability,the accelerated degradation testing(ADT)has been proposed.Composite wind turbine blade is one of the most important co...In order to predict the lifetime of products appropriately with long lifetime and high reliability,the accelerated degradation testing(ADT)has been proposed.Composite wind turbine blade is one of the most important components in wind turbine system.Its fatigue cycle is very long in practice.A full-scale fatigue testing is usually used to verify the design of a new blade.In general,the full-scale fatigue testing of blade is accelerated on the basis of the damage equivalent principle.During the full-scale fatigue test ing,blade is subjected to higher testing load than normal operat ing conditions;consequently,the performance degradation of the blade is hastened over time.The full-scale fatigue testing of blade is regarded as a special ADT.According to the fatigue failure criterion,we choose blade stiffness as the characteristic quantity of the blade performance,and propose an accelerated model(AM)for blade on the basis of the theories of ADT.Then,degradation path of the blade stiffness is modeled by using Gamma process.Finally,the lifet ime prediction of full-scale megawatt(MW)blade is conducted by combining the proposed AM and blade stiffness degradation model.The prediction results prove the reasonability and validity of this study.This can supply a new approach to predict the lifetime of the full-scale MW blade.展开更多
The aim of this er vito study was to explore the potential of using the fluorescence lifetime of intraellular reduced nicotinamide adenine dinucleotide(phosphate)(NAD(P)H)as a label-free indicator to characterize the ...The aim of this er vito study was to explore the potential of using the fluorescence lifetime of intraellular reduced nicotinamide adenine dinucleotide(phosphate)(NAD(P)H)as a label-free indicator to characterize the di ferencs between human leukemic myeloid cells and normal mononuclear cells(MNC).The steady-state and time-resolved autofuorescence of two human leukemic myeloid cell lines(K562,HL60)and MNC were measured by a spectrofuorimeter.According to excitation-enmission matrix(EEM)analysis,the optimal emission of NAD(P)H in these cells suspensions occurred at 445 nm.Furthermore,the fuorescence lifetimes of NAD(P)H in leukemic myeloid cells and MNC were determined by fitting the time-resolved autofuorescence data.The mean fuorescence lifetimes of NAD(P)H in K562,HL60,and MNC cells were 557±1.19,4.45±0.71,and 7.31±0.60 ns,respectively.There was a significant diference in the mean lifetime of NAD(P)H between leukemic myeloid cells and MNC(p<0.05).The difference was essentally caused by the change in relative concentration of free and protein-bound NAD(P)H.This study suggests that the mean fuorescence lifetime of NAD(P)H might be a potential label-free indicator for differentiating leukemic myeloid cells from MNC.展开更多
基金the National Natural Science Founda-tion of China(No.51665029)。
文摘In order to predict the lifetime of products appropriately with long lifetime and high reliability,the accelerated degradation testing(ADT)has been proposed.Composite wind turbine blade is one of the most important components in wind turbine system.Its fatigue cycle is very long in practice.A full-scale fatigue testing is usually used to verify the design of a new blade.In general,the full-scale fatigue testing of blade is accelerated on the basis of the damage equivalent principle.During the full-scale fatigue test ing,blade is subjected to higher testing load than normal operat ing conditions;consequently,the performance degradation of the blade is hastened over time.The full-scale fatigue testing of blade is regarded as a special ADT.According to the fatigue failure criterion,we choose blade stiffness as the characteristic quantity of the blade performance,and propose an accelerated model(AM)for blade on the basis of the theories of ADT.Then,degradation path of the blade stiffness is modeled by using Gamma process.Finally,the lifet ime prediction of full-scale megawatt(MW)blade is conducted by combining the proposed AM and blade stiffness degradation model.The prediction results prove the reasonability and validity of this study.This can supply a new approach to predict the lifetime of the full-scale MW blade.
基金supported by the National Natural Science Foundation of China(61275216)the Fujian Provincial Natural Science Foundation(2011J06022)+1 种基金the Science Research foundation of Ministry of Health&United Fujian Provincial Health and Education Project for Tackling the Key Research(WKJ2008-2-049)the Program for Changjiang Scholars and Innovative Research Team in University(IRT1115).
文摘The aim of this er vito study was to explore the potential of using the fluorescence lifetime of intraellular reduced nicotinamide adenine dinucleotide(phosphate)(NAD(P)H)as a label-free indicator to characterize the di ferencs between human leukemic myeloid cells and normal mononuclear cells(MNC).The steady-state and time-resolved autofuorescence of two human leukemic myeloid cell lines(K562,HL60)and MNC were measured by a spectrofuorimeter.According to excitation-enmission matrix(EEM)analysis,the optimal emission of NAD(P)H in these cells suspensions occurred at 445 nm.Furthermore,the fuorescence lifetimes of NAD(P)H in leukemic myeloid cells and MNC were determined by fitting the time-resolved autofuorescence data.The mean fuorescence lifetimes of NAD(P)H in K562,HL60,and MNC cells were 557±1.19,4.45±0.71,and 7.31±0.60 ns,respectively.There was a significant diference in the mean lifetime of NAD(P)H between leukemic myeloid cells and MNC(p<0.05).The difference was essentally caused by the change in relative concentration of free and protein-bound NAD(P)H.This study suggests that the mean fuorescence lifetime of NAD(P)H might be a potential label-free indicator for differentiating leukemic myeloid cells from MNC.
