目的建立肉豆蔻Myristicae Semen多指标成分定量检测方法,构建化学计量学及加权逼近理想排序(TOPSIS)模型,对不同产地肉豆蔻进行药材质量评价。方法收集5省16批次肉豆蔻样品,以去氢二异丁香酚和β-谷甾醇为内参物,采用一测多评(quantita...目的建立肉豆蔻Myristicae Semen多指标成分定量检测方法,构建化学计量学及加权逼近理想排序(TOPSIS)模型,对不同产地肉豆蔻进行药材质量评价。方法收集5省16批次肉豆蔻样品,以去氢二异丁香酚和β-谷甾醇为内参物,采用一测多评(quantitative analysis of multi-components by single marker,QAMS)法同步测定肉豆蔻中安五脂素、肉豆蔻木脂素、去氢二异丁香酚、利卡灵-B、丁香酚、异丁香酚、肉豆蔻醚、榄香脂素、β-谷甾醇和豆甾醇含量,同时对其醇溶性浸出物、总灰分和酸不溶性灰分进行定量检测。采用主成分分析对不同产地肉豆蔻进行聚类分组,以因子分析主成分综合得分高低对样品质量优劣进行排序,通过正交偏最小二乘法-判别分析挖掘影响肉豆蔻产品质量的主要潜在标志物,同时构建加权TOPSIS质量优劣评价模型,对肉豆蔻质量差异性进行综合评价。结果安五脂素、肉豆蔻木脂素、去氢二异丁香酚、利卡灵-B、丁香酚、异丁香酚、肉豆蔻醚、榄香脂素、β-谷甾醇、豆甾醇分别在8.55~427.50、7.17~358.50、4.85~242.50、0.14~7.00、0.53~26.50、3.55~177.50、2.97~148.50、6.45~322.50、0.36~18.00和0.22~11.00μg/mL线性关系良好(r>0.999),平均加样回收率96.92%~100.11%(RSD<2.0%);建立的相对校正因子耐用性良好,外标法与QAMS法所得含量结果无明显差异。16批样品聚为3类,产地相近的聚为一组;安五脂素、榄香脂素、肉豆蔻木脂素、肉豆蔻醚、去氢二异丁香酚、β-谷甾醇和异丁香酚可能是影响肉豆蔻产品质量主要潜在标志物;因子分析与加权TOPSIS模型样品质量优劣排序结果基本一致。结论QAMS多指标成分定量方法操作简便、结果准确,化学计量学及加权TOPSIS模型结果科学直观,可用于不同产地肉豆蔻药材质量评价。展开更多
Aim Isolation and structural elucidation of the constituents from the dried flower buds of Magnolia biondii Pamp. Methods Column chromatography and TLC were used to isolate chemical constituents. Spectroscopic methods...Aim Isolation and structural elucidation of the constituents from the dried flower buds of Magnolia biondii Pamp. Methods Column chromatography and TLC were used to isolate chemical constituents. Spectroscopic methods were employed for structural elucidation. Results One benzo furanoid neolignan （licarin B） and two bisepoxy lignans （magnolin, fargesin） were isolated and identified. Conclusion Licarin B is the first reported benzofuranoid lignan from the family Magnoliaceae.展开更多
文摘目的建立肉豆蔻Myristicae Semen多指标成分定量检测方法,构建化学计量学及加权逼近理想排序(TOPSIS)模型,对不同产地肉豆蔻进行药材质量评价。方法收集5省16批次肉豆蔻样品,以去氢二异丁香酚和β-谷甾醇为内参物,采用一测多评(quantitative analysis of multi-components by single marker,QAMS)法同步测定肉豆蔻中安五脂素、肉豆蔻木脂素、去氢二异丁香酚、利卡灵-B、丁香酚、异丁香酚、肉豆蔻醚、榄香脂素、β-谷甾醇和豆甾醇含量,同时对其醇溶性浸出物、总灰分和酸不溶性灰分进行定量检测。采用主成分分析对不同产地肉豆蔻进行聚类分组,以因子分析主成分综合得分高低对样品质量优劣进行排序,通过正交偏最小二乘法-判别分析挖掘影响肉豆蔻产品质量的主要潜在标志物,同时构建加权TOPSIS质量优劣评价模型,对肉豆蔻质量差异性进行综合评价。结果安五脂素、肉豆蔻木脂素、去氢二异丁香酚、利卡灵-B、丁香酚、异丁香酚、肉豆蔻醚、榄香脂素、β-谷甾醇、豆甾醇分别在8.55~427.50、7.17~358.50、4.85~242.50、0.14~7.00、0.53~26.50、3.55~177.50、2.97~148.50、6.45~322.50、0.36~18.00和0.22~11.00μg/mL线性关系良好(r>0.999),平均加样回收率96.92%~100.11%(RSD<2.0%);建立的相对校正因子耐用性良好,外标法与QAMS法所得含量结果无明显差异。16批样品聚为3类,产地相近的聚为一组;安五脂素、榄香脂素、肉豆蔻木脂素、肉豆蔻醚、去氢二异丁香酚、β-谷甾醇和异丁香酚可能是影响肉豆蔻产品质量主要潜在标志物;因子分析与加权TOPSIS模型样品质量优劣排序结果基本一致。结论QAMS多指标成分定量方法操作简便、结果准确,化学计量学及加权TOPSIS模型结果科学直观,可用于不同产地肉豆蔻药材质量评价。
文摘Aim Isolation and structural elucidation of the constituents from the dried flower buds of Magnolia biondii Pamp. Methods Column chromatography and TLC were used to isolate chemical constituents. Spectroscopic methods were employed for structural elucidation. Results One benzo furanoid neolignan （licarin B） and two bisepoxy lignans （magnolin, fargesin） were isolated and identified. Conclusion Licarin B is the first reported benzofuranoid lignan from the family Magnoliaceae.
基金云南省科技厅-云南省地方本科高校基础研究联合专项项目(2017FH001-094 and 2017FH001-092)云南省科技厅应用基础研究青年项目(2018FD081)+1 种基金云南省教育厅科学研究基金资助性项目(2016ZZX208)地方高校国家级大学生创新创业训练计划项目(201610684010)。
