With the increasing accumulation of plastic pollutants in various environments,research on microorganisms(including bacteria,fungi,and algae)with plastic degradation capabilities has gained significant attention.Howev...With the increasing accumulation of plastic pollutants in various environments,research on microorganisms(including bacteria,fungi,and algae)with plastic degradation capabilities has gained significant attention.However,only a limited number of microbial plastic-degrading enzymes have been identified to date.This highlights that the degradation mechanisms employed by many plastic-degrading microorganisms,particularly filamentous fungi,remain insufficiently explored.In this study,we utilized a versatile fungal plasmid(pCT74)to express green fluorescent protein(GFP)in a marine-derived fungus Alternaria alternata strain FB1 with plastic degradation capabilities.Upon evaluating the degradation effect of polyester-type polyurethane(PU)film,we observed that different transformants exhibited three kinds of activities(the same,reduced,or enhanced degradation capability)compared to the FB1 wild-type strain.Further analysis of the plasmid fragment insertion sites in different transformants revealed that pCT74 integrates randomly into the genome of the host fungus.Notably,a direct correlation was found between the plasmid insertion site and the degradation capability of the corresponding transformant.Our findings not only redefine the potential applications of plasmid pCT74 in filamentous fungi but also show a novel research approach to identifying key enzymes involved in plastic degradation by fungi.展开更多
Ferroptosis of chondrocytes is a significant contributor to osteoarthritis(OA),for which there is still a lack of safe and effective therapeutic drugs targeting ferroptosis.Here,we screen for anti-ferroptotic drugs in...Ferroptosis of chondrocytes is a significant contributor to osteoarthritis(OA),for which there is still a lack of safe and effective therapeutic drugs targeting ferroptosis.Here,we screen for anti-ferroptotic drugs in Food and Drug Administration(FDA)-approved drug library via a high-throughput manner in chondrocytes.We identified a group of FDA-approved anti-ferroptotic drugs,among which vitamin K showed the most powerful protective effect.Further study demonstrated that vitamin K effectively inhibited ferroptosis and alleviated the extracellular matrix(ECM)degradation in chondrocytes.Intra-articular injection of vitamin K inhibited ferroptosis and alleviated OA phenotype in destabilization of the medial meniscus(DMM)mouse model.Mechanistically,transcriptome sequencing and knockdown experiments revealed that the anti-ferroptotic effects of vitamin K depended on growth arrest-specific 6(Gas6).Furthermore,exogenous expression of Gas6 was found to inhibit ferroptosis through the AXL receptor tyrosine kinase(AXL)/phosphatidylinositol 3-kinase(PI3K)/AKT serine/threonine kinase(AKT)axis.Together,we demonstrate that vitamin K inhibits ferroptosis and alleviates OA progression via enhancing Gas6 expression and its downstream pathway of AXL/PI3K/AKT axis,indicating vitamin K as well as Gas6 to serve as a potential therapeutic target for OA and other ferroptosis-related diseases.展开更多
With rapid technological advancements,academic libraries are compelled to reevaluate their mission statements and to reinmagine their roles in higher education institutions.The integration of innovative technologies i...With rapid technological advancements,academic libraries are compelled to reevaluate their mission statements and to reinmagine their roles in higher education institutions.The integration of innovative technologies in the learning and academic work has transformed user relationships with academic libraries in many ways.The paper examines the initiatives that a school-based library has undertaken to accommodate students’needs in light of emerging technologies.These initiatives involve space allocation,campus-wide programs and activities,and the provision of information-related services.The resulting benefits for academic user engagement and the current challenges and opportunities for academic libraries are discussed in the paper.展开更多
The aim of this article is to present a unique interpretation of the holistic worldview,both in theory and in practice.It explores how this approach,along with a planning process fundamentally different from conventio...The aim of this article is to present a unique interpretation of the holistic worldview,both in theory and in practice.It explores how this approach,along with a planning process fundamentally different from conventional methods,was implemented in the design and construction of the Music Center and Library in the city of Tel Aviv.This process forms a coherent representation of a complete worldview:a humanistic,holistic worldview developed and adopted through more than five decades of architectural practice across all scales of design.In this approach,a building is not regarded as a collection of isolated design elements,but as one hierarchical language,in which the building,its interior,and its ornamented details down to the handle of the door is one continuous system.Within this system,the building,its interior spaces,and even the smallest ornamental details,down to the door handles,are conceived as parts of a single,continuous whole.This worldview aligns with contemporary scientific discourse in fields such as cosmology,neurobiology,psychology,complexity theory,and Buddhist philosophy,disciplines with which this body of work is closely associated.展开更多
This paper conducts topic mining and analysis of research literature in the domestic smart library field based on the BERTopic model,aiming to reveal its topic development context and evolution trends.Journal literatu...This paper conducts topic mining and analysis of research literature in the domestic smart library field based on the BERTopic model,aiming to reveal its topic development context and evolution trends.Journal literature in the smart library field collected by CNKI(China National Knowledge Infrastructure)from 2015 to 2024 was analyzed using the BERTopic model and dynamic topic modeling for topic mining and evolution trend analysis.The study found that the domestic smart library field involves multiple core topics,identifying a diversified topic structure centered around“data”,“user”,“5g”,etc.The research results provide data support and practical reference for libraries to accurately identify key points of technology integration during smart transformation and to optimize smart service models.展开更多
Data factors are becoming the core driving force in the intelligent transformation of libraries.Based on a systematic review of the progress in data governance practices in libraries both domestically and internationa...Data factors are becoming the core driving force in the intelligent transformation of libraries.Based on a systematic review of the progress in data governance practices in libraries both domestically and internationally,this study delves into the mechanism by which data governance promotes data factorization and proposes implementation paths for data governance oriented toward data factorization.The aim is to facilitate the intelligent transformation and high-quality development of libraries.展开更多
Building on the self-regulation theory and the gap model of service quality,this study proposes a conceptual model for investigating the help-seeking behavior of library users and the behaviors’underlying mechanisms ...Building on the self-regulation theory and the gap model of service quality,this study proposes a conceptual model for investigating the help-seeking behavior of library users and the behaviors’underlying mechanisms in the context of university libraries.The study focuses on university students as the research participants,and employs a questionnaire survey to collect data.The data is then analyzed,and the model is evaluated using the regression and bootstrap methods.The results indicate that library anxiety can lead to help-seeking behavior,while it also diminishes the users’perception of their interaction quality with the librarians.Moreover,the interaction quality positively influences help-seeking behavior,and self-efficacy and artificial intelligence services exhibit both direct and indirect negative regulatory effects.展开更多
As an important method of practical teaching,case-based teaching has become increasingly prominent in international Chinese language education.However,the development of case-based teaching for Chinese characters rema...As an important method of practical teaching,case-based teaching has become increasingly prominent in international Chinese language education.However,the development of case-based teaching for Chinese characters remains insufficient,with limited research outcomes,making it difficult to effectively support the teaching and research of Chinese character instruction.The establishment of a case library for international Chinese character teaching can provide a wealth of teaching cases,meeting the developmental needs of international Chinese language education in the new era.展开更多
Objective:To enhance the reading skills of clinical pathology residents,it is essential to establish a well-structured electronic pathology reading library.Methods:In accordance with the Resident Standardization Train...Objective:To enhance the reading skills of clinical pathology residents,it is essential to establish a well-structured electronic pathology reading library.Methods:In accordance with the Resident Standardization Training Content and Standards(2022 Edition),clinical pathology residents are required to master pathological diagnoses across 11 systems:skin,head and neck,mediastinum and respiratory,digestive,urinary and male reproductive,female reproductive and breast,lymphatic and hematopoietic,bone and soft tissue,cardiovascular,central nervous,and endocrine diseases.Senior pathologists specializing in each subspecialty selected classic pathological slides,which were systematically scanned and compiled into an electronic pathology library.Results:A questionnaire survey was conducted to gather feedback on the electronic pathology reading library.Residents generally found it to be convenient,efficient,and conducive to learning.Conclusion:Training in clinical pathology diagnosis is a core component of standardized resident training.The electronic pathology reading library has been well-received and recognized by resident doctors.However,further efforts are needed to explore diverse teaching methods that align with modern educational approaches,ultimately contributing to the development of highly skilled resident doctors.展开更多
通过对国内外Living Library的考察,结合Web2.0和社会性网络SNS的发展,提出了一个基于SNS的Living Library虚拟社区模型:Virtual Living Library(简称VLL),并就VLL的概念模型的构建、实践和评价等方面进行了论述,讨论了VLL的建设可能性...通过对国内外Living Library的考察,结合Web2.0和社会性网络SNS的发展,提出了一个基于SNS的Living Library虚拟社区模型:Virtual Living Library(简称VLL),并就VLL的概念模型的构建、实践和评价等方面进行了论述,讨论了VLL的建设可能性和发展前景。展开更多
基金Supported by the Science and Technology Innovation Project of Laoshan Laboratory(Nos.2022QNLM030004-3,LSKJ202203103)the NSFC Innovative Group Grant(No.42221005)+5 种基金the Key Collaborative Research Program of the Alliance of International Science Organizations(No.ANSO-CR-KP-2022-08)the Shandong Provincial Natural Science Foundation(No.ZR2021ZD28)the Major Research Plan of the National Natural Science Foundation(No.92351301)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA22050301)the Taishan Scholars Program(No.tstp20230637)the Qingdao Natural Science Foundation(No.23-2-1-182-zyyd-jch)。
文摘With the increasing accumulation of plastic pollutants in various environments,research on microorganisms(including bacteria,fungi,and algae)with plastic degradation capabilities has gained significant attention.However,only a limited number of microbial plastic-degrading enzymes have been identified to date.This highlights that the degradation mechanisms employed by many plastic-degrading microorganisms,particularly filamentous fungi,remain insufficiently explored.In this study,we utilized a versatile fungal plasmid(pCT74)to express green fluorescent protein(GFP)in a marine-derived fungus Alternaria alternata strain FB1 with plastic degradation capabilities.Upon evaluating the degradation effect of polyester-type polyurethane(PU)film,we observed that different transformants exhibited three kinds of activities(the same,reduced,or enhanced degradation capability)compared to the FB1 wild-type strain.Further analysis of the plasmid fragment insertion sites in different transformants revealed that pCT74 integrates randomly into the genome of the host fungus.Notably,a direct correlation was found between the plasmid insertion site and the degradation capability of the corresponding transformant.Our findings not only redefine the potential applications of plasmid pCT74 in filamentous fungi but also show a novel research approach to identifying key enzymes involved in plastic degradation by fungi.
基金supported by grants from the Wenzhou Science and Technology Bureau Foundation,China(Grant No.:ZY2019014)“Pioneer”and“Leading Goose”R&D Program of Zhejiang,China(Grant No.:2022C03144)National Natural Science Foundation of China(Grant Nos.:82172494,and 82372461).
文摘Ferroptosis of chondrocytes is a significant contributor to osteoarthritis(OA),for which there is still a lack of safe and effective therapeutic drugs targeting ferroptosis.Here,we screen for anti-ferroptotic drugs in Food and Drug Administration(FDA)-approved drug library via a high-throughput manner in chondrocytes.We identified a group of FDA-approved anti-ferroptotic drugs,among which vitamin K showed the most powerful protective effect.Further study demonstrated that vitamin K effectively inhibited ferroptosis and alleviated the extracellular matrix(ECM)degradation in chondrocytes.Intra-articular injection of vitamin K inhibited ferroptosis and alleviated OA phenotype in destabilization of the medial meniscus(DMM)mouse model.Mechanistically,transcriptome sequencing and knockdown experiments revealed that the anti-ferroptotic effects of vitamin K depended on growth arrest-specific 6(Gas6).Furthermore,exogenous expression of Gas6 was found to inhibit ferroptosis through the AXL receptor tyrosine kinase(AXL)/phosphatidylinositol 3-kinase(PI3K)/AKT serine/threonine kinase(AKT)axis.Together,we demonstrate that vitamin K inhibits ferroptosis and alleviates OA progression via enhancing Gas6 expression and its downstream pathway of AXL/PI3K/AKT axis,indicating vitamin K as well as Gas6 to serve as a potential therapeutic target for OA and other ferroptosis-related diseases.
文摘With rapid technological advancements,academic libraries are compelled to reevaluate their mission statements and to reinmagine their roles in higher education institutions.The integration of innovative technologies in the learning and academic work has transformed user relationships with academic libraries in many ways.The paper examines the initiatives that a school-based library has undertaken to accommodate students’needs in light of emerging technologies.These initiatives involve space allocation,campus-wide programs and activities,and the provision of information-related services.The resulting benefits for academic user engagement and the current challenges and opportunities for academic libraries are discussed in the paper.
文摘The aim of this article is to present a unique interpretation of the holistic worldview,both in theory and in practice.It explores how this approach,along with a planning process fundamentally different from conventional methods,was implemented in the design and construction of the Music Center and Library in the city of Tel Aviv.This process forms a coherent representation of a complete worldview:a humanistic,holistic worldview developed and adopted through more than five decades of architectural practice across all scales of design.In this approach,a building is not regarded as a collection of isolated design elements,but as one hierarchical language,in which the building,its interior,and its ornamented details down to the handle of the door is one continuous system.Within this system,the building,its interior spaces,and even the smallest ornamental details,down to the door handles,are conceived as parts of a single,continuous whole.This worldview aligns with contemporary scientific discourse in fields such as cosmology,neurobiology,psychology,complexity theory,and Buddhist philosophy,disciplines with which this body of work is closely associated.
文摘This paper conducts topic mining and analysis of research literature in the domestic smart library field based on the BERTopic model,aiming to reveal its topic development context and evolution trends.Journal literature in the smart library field collected by CNKI(China National Knowledge Infrastructure)from 2015 to 2024 was analyzed using the BERTopic model and dynamic topic modeling for topic mining and evolution trend analysis.The study found that the domestic smart library field involves multiple core topics,identifying a diversified topic structure centered around“data”,“user”,“5g”,etc.The research results provide data support and practical reference for libraries to accurately identify key points of technology integration during smart transformation and to optimize smart service models.
文摘Data factors are becoming the core driving force in the intelligent transformation of libraries.Based on a systematic review of the progress in data governance practices in libraries both domestically and internationally,this study delves into the mechanism by which data governance promotes data factorization and proposes implementation paths for data governance oriented toward data factorization.The aim is to facilitate the intelligent transformation and high-quality development of libraries.
基金supported by the Major Project of the Sichuan Academy of Social Sciences(Project No.24ZD04).
文摘Building on the self-regulation theory and the gap model of service quality,this study proposes a conceptual model for investigating the help-seeking behavior of library users and the behaviors’underlying mechanisms in the context of university libraries.The study focuses on university students as the research participants,and employs a questionnaire survey to collect data.The data is then analyzed,and the model is evaluated using the regression and bootstrap methods.The results indicate that library anxiety can lead to help-seeking behavior,while it also diminishes the users’perception of their interaction quality with the librarians.Moreover,the interaction quality positively influences help-seeking behavior,and self-efficacy and artificial intelligence services exhibit both direct and indirect negative regulatory effects.
基金the research result of the 2022 International Chinese Education Research Project“Construction and Research of International Chinese Character Teaching Case Library in Chinese Education”(22YH88D).
文摘As an important method of practical teaching,case-based teaching has become increasingly prominent in international Chinese language education.However,the development of case-based teaching for Chinese characters remains insufficient,with limited research outcomes,making it difficult to effectively support the teaching and research of Chinese character instruction.The establishment of a case library for international Chinese character teaching can provide a wealth of teaching cases,meeting the developmental needs of international Chinese language education in the new era.
文摘Objective:To enhance the reading skills of clinical pathology residents,it is essential to establish a well-structured electronic pathology reading library.Methods:In accordance with the Resident Standardization Training Content and Standards(2022 Edition),clinical pathology residents are required to master pathological diagnoses across 11 systems:skin,head and neck,mediastinum and respiratory,digestive,urinary and male reproductive,female reproductive and breast,lymphatic and hematopoietic,bone and soft tissue,cardiovascular,central nervous,and endocrine diseases.Senior pathologists specializing in each subspecialty selected classic pathological slides,which were systematically scanned and compiled into an electronic pathology library.Results:A questionnaire survey was conducted to gather feedback on the electronic pathology reading library.Residents generally found it to be convenient,efficient,and conducive to learning.Conclusion:Training in clinical pathology diagnosis is a core component of standardized resident training.The electronic pathology reading library has been well-received and recognized by resident doctors.However,further efforts are needed to explore diverse teaching methods that align with modern educational approaches,ultimately contributing to the development of highly skilled resident doctors.
文摘通过对国内外Living Library的考察,结合Web2.0和社会性网络SNS的发展,提出了一个基于SNS的Living Library虚拟社区模型:Virtual Living Library(简称VLL),并就VLL的概念模型的构建、实践和评价等方面进行了论述,讨论了VLL的建设可能性和发展前景。
