Fluorescence lateral flow immunoassay(LFA)has emerged as a powerful tool for rapid screening of various biomarkers owing to its simplicity,sensitivity and flexibility.It is noteworthy that fluorescent probe mainly det...Fluorescence lateral flow immunoassay(LFA)has emerged as a powerful tool for rapid screening of various biomarkers owing to its simplicity,sensitivity and flexibility.It is noteworthy that fluorescent probe mainly determines the analytical performance of LFA.Due to the emission and excitation wavelengths are located in the visible region,most fluorophores are inevitably subject to light scattering and background autofluorescence.Herein,we reported a novel LFA sensor based on the second near-infrared(NIR-Ⅱ)fluorescent probe with excellent anti-interference capability.The designed NIR-Ⅱprobe was the Nd^(3+)and Yb^(3+)doped rare earth nanoparticles(RENPs)by employing Nd^(3+)as energy donor and Yb^(3+)as energy acceptor,which of the donor-acceptor energy transfer(ET)efficiency reached up to 80.7%.Meanwhile,relying on the convenient and effective encapsulation strategy of poly(lactic-co-glycolic acid)(PLGA)microspheres to RENPs,the surface functionalized NIR-Ⅱprobe(RE@PLGA)was obtained for subsequent bioconjugation.Benefiting from the optical advantages of NIR-Ⅱprobe,this proposed NIR-ⅡLFA displayed a good linear relationship ranging from 7 ng/mL to 200 ng/mL for the detection ofα-fetoprotein(AFP),an important biomarker of hepatocellular carcinoma(HCC).The limit of detection(LOD)was determined as low as 3.0 ng/m L,which was of 8.3 times lower than clinical cutoff value.It is promising that LFA sensor based on this efficient RENPs probe provides new opportunities for high sensitive detection of various biomarkers in biological samples.展开更多
Background:Organ transplantation recipients encounter significant risks from acute or chronic infections that threaten graft survival.BK virus(BKV)and JC virus(JCV)are 2 prominent opportunistic infection viruses,and t...Background:Organ transplantation recipients encounter significant risks from acute or chronic infections that threaten graft survival.BK virus(BKV)and JC virus(JCV)are 2 prominent opportunistic infection viruses,and they may cause polyomavirus-associated nephropathy and graft kidney loss in patients who are in an immunosuppressed state after kidney transplantation.Hence,timely detection and sustained monitoring of the viral load are indispensable.However,the current diagnostic methods remain limited,and the development of new molecular detection technology is extremely urgent.Methods:The sequences and concentrations of clustered regularly interspaced short palindromic repeats(CRISPR)RNA(crRNA),the concentration of Cas13a,and the primers for recombinase polymerase amplification(RPA)were optimized for BKV and JCV detection.Next,a novel microfluidic dual-droplet chip was designed and fabricated,and it was integrated with CRISPR(ddCRISPR)to simultaneously qualitatively detect BKV and JCV.Subsequently,the ddCRISPR assay was verified using clinical samples.Then,a lateral flow strip combined with CRISPR(LFCRISPR)was developed for the detection of BKV and JCV in resource-limited settings.Results:A one-pot RPA-CRISPR reaction system was established and optimized for BKV and JCV detection.ddCRISPR can simultaneously and rapidly detect BKV and JCV with high sensitivity(10 copies/ml for BKV and 1 copy/ml for JCV),and provide absolute quantification,which is suitable for viral load detection and conducive to personalized and precise treatment for organ transplant recipients.LFCRISPR simplified the operational process through a simple visual readout,facilitating virus screening after organ transplantation.Conclusion:These platforms incorporate molecular testing into the transplantation treatment model,thereby reducing costs,prolonging the survival time of the graft,improving the clinical outcomes of postoperative management in kidney transplantation,and enhancing the patients’quality of life.展开更多
采用LFA(Landscape Function Analysis)方法,通过调查7种地类35组样本,评价植被类型、人类活动对林地景观功能和土壤性能的影响,并验证LFA方法在黄土区的适用性。结果表明:不同植被类型对林地土壤结构稳定性、渗透性能、养分循环性能的...采用LFA(Landscape Function Analysis)方法,通过调查7种地类35组样本,评价植被类型、人类活动对林地景观功能和土壤性能的影响,并验证LFA方法在黄土区的适用性。结果表明:不同植被类型对林地土壤结构稳定性、渗透性能、养分循环性能的影响显著,其中,天然次生林改良土壤结构稳定性、渗透性和养分循环性能的作用最好,其次是刺槐人工林、油松人工林,荒草坡最差;人类活动对人工林地景观功能和林地土壤结构稳定性有显著影响;LFA方法能够较好地评价植被类型对林地土壤性能的影响,以及人类活动对人工林地景观功能和林地土壤结构稳定性的影响,在黄土残塬沟壑区有较好的适用性。展开更多
基金supported by the National Natural Science Foundation of China(Nos.U2267221,22107029,22377135)the Bohai Rim Advanced Research Institute for Drug Discovery(No.LX215002)+5 种基金the Natural Science Foundation of Shandong Province(No.ZR2022QH212)the Taishan Scholars Program(No.tsqn202312305)the Young Elite Scientists Sponsorship Program by Chinese Chemical Societythe Fundamental Research Projects of Science&Technology Innovation and development Plan in Yantai City(No.2023JCYJ059)the Shandong Laboratory Program(No.SYS202205)the Shanghai Postdoctoral Excellence Program(No.2023704)。
文摘Fluorescence lateral flow immunoassay(LFA)has emerged as a powerful tool for rapid screening of various biomarkers owing to its simplicity,sensitivity and flexibility.It is noteworthy that fluorescent probe mainly determines the analytical performance of LFA.Due to the emission and excitation wavelengths are located in the visible region,most fluorophores are inevitably subject to light scattering and background autofluorescence.Herein,we reported a novel LFA sensor based on the second near-infrared(NIR-Ⅱ)fluorescent probe with excellent anti-interference capability.The designed NIR-Ⅱprobe was the Nd^(3+)and Yb^(3+)doped rare earth nanoparticles(RENPs)by employing Nd^(3+)as energy donor and Yb^(3+)as energy acceptor,which of the donor-acceptor energy transfer(ET)efficiency reached up to 80.7%.Meanwhile,relying on the convenient and effective encapsulation strategy of poly(lactic-co-glycolic acid)(PLGA)microspheres to RENPs,the surface functionalized NIR-Ⅱprobe(RE@PLGA)was obtained for subsequent bioconjugation.Benefiting from the optical advantages of NIR-Ⅱprobe,this proposed NIR-ⅡLFA displayed a good linear relationship ranging from 7 ng/mL to 200 ng/mL for the detection ofα-fetoprotein(AFP),an important biomarker of hepatocellular carcinoma(HCC).The limit of detection(LOD)was determined as low as 3.0 ng/m L,which was of 8.3 times lower than clinical cutoff value.It is promising that LFA sensor based on this efficient RENPs probe provides new opportunities for high sensitive detection of various biomarkers in biological samples.
基金supported by the National Key Research and Development Program(2023YFC2306200)the National Natural Science Foundation of China(82472374)+1 种基金the Cross-Research Fund of Biomedical Engineering of Shanghai Jiaotong University(YG2024LC02)the Clinical Excellence project of Shanghai Key Laboratory of Nucleic Acid Chemistry and Nanomedicine(2024ZY008).
文摘Background:Organ transplantation recipients encounter significant risks from acute or chronic infections that threaten graft survival.BK virus(BKV)and JC virus(JCV)are 2 prominent opportunistic infection viruses,and they may cause polyomavirus-associated nephropathy and graft kidney loss in patients who are in an immunosuppressed state after kidney transplantation.Hence,timely detection and sustained monitoring of the viral load are indispensable.However,the current diagnostic methods remain limited,and the development of new molecular detection technology is extremely urgent.Methods:The sequences and concentrations of clustered regularly interspaced short palindromic repeats(CRISPR)RNA(crRNA),the concentration of Cas13a,and the primers for recombinase polymerase amplification(RPA)were optimized for BKV and JCV detection.Next,a novel microfluidic dual-droplet chip was designed and fabricated,and it was integrated with CRISPR(ddCRISPR)to simultaneously qualitatively detect BKV and JCV.Subsequently,the ddCRISPR assay was verified using clinical samples.Then,a lateral flow strip combined with CRISPR(LFCRISPR)was developed for the detection of BKV and JCV in resource-limited settings.Results:A one-pot RPA-CRISPR reaction system was established and optimized for BKV and JCV detection.ddCRISPR can simultaneously and rapidly detect BKV and JCV with high sensitivity(10 copies/ml for BKV and 1 copy/ml for JCV),and provide absolute quantification,which is suitable for viral load detection and conducive to personalized and precise treatment for organ transplant recipients.LFCRISPR simplified the operational process through a simple visual readout,facilitating virus screening after organ transplantation.Conclusion:These platforms incorporate molecular testing into the transplantation treatment model,thereby reducing costs,prolonging the survival time of the graft,improving the clinical outcomes of postoperative management in kidney transplantation,and enhancing the patients’quality of life.
文摘采用LFA(Landscape Function Analysis)方法,通过调查7种地类35组样本,评价植被类型、人类活动对林地景观功能和土壤性能的影响,并验证LFA方法在黄土区的适用性。结果表明:不同植被类型对林地土壤结构稳定性、渗透性能、养分循环性能的影响显著,其中,天然次生林改良土壤结构稳定性、渗透性和养分循环性能的作用最好,其次是刺槐人工林、油松人工林,荒草坡最差;人类活动对人工林地景观功能和林地土壤结构稳定性有显著影响;LFA方法能够较好地评价植被类型对林地土壤性能的影响,以及人类活动对人工林地景观功能和林地土壤结构稳定性的影响,在黄土残塬沟壑区有较好的适用性。
