A crossbreeding program was established in 2019 to address the declining Crassostrea gigas harvests caused by Pacific Oyster Mortality Syndrome(POMS).As a part of the program,this study was performed to estimate the g...A crossbreeding program was established in 2019 to address the declining Crassostrea gigas harvests caused by Pacific Oyster Mortality Syndrome(POMS).As a part of the program,this study was performed to estimate the genetic structure underlying phenotypic variation.Fifteen complete diallel crossings of C.gigas and C.angulata,comprising 60 full-sib families,were used to evaluate the general combining ability(GCA)and specific combining ability(SCA),as well as genotype and environment interactions for shell height(SH),summer survival(SS),and thermal tolerance(TT)of reciprocal hybrids GA(C.gigas♀×C.angulata♂)and AG(C.angulata♀×C.gigas♂)grown in Rongcheng and Rushan,Shandong Province,China.The results suggested that heterosis of the reciprocal hybrids was evident for SH,SS,and TT.The hybrid GA had larger heterosis than AG in both testing environments,and can be a potential donor in the breeding program.The male C.gigas had better GCA for SH in Rongcheng,whereas male C.angulata was a good general combiner for SS and TT in both Rongcheng and Rushan.The estimate of SCA was much higher than GCA for SH and lower than GCA for TT.To harness both additive and non-additive genetic effects,combination breed-ing could be taken to develop hybrid varieties possessing both thermal tolerance and fast-growing traits.The positive correlations between SH and TT suggested that these traits could be improved simultaneously.The significant G×E interactions demonstrated the importance to undertake site-specific breeding programs in different environments.Overall,this study can provide essential information for developing crossbreeding strategies for the oyster farming industry.展开更多
This paper focuses on the transformation of the project-based learning(PBL)teaching model driven by artificial intelligence(AI),and explores the two-way reconstruction path of teacher roles and student abilities.Combi...This paper focuses on the transformation of the project-based learning(PBL)teaching model driven by artificial intelligence(AI),and explores the two-way reconstruction path of teacher roles and student abilities.Combining metacognitive theory to analyze the pain points of traditional PBL,this paper systematically sorts out the functional reconstruction path of AI in the dimensions of teaching design,process monitoring,and evaluation feedback.Then,starting from the social role theory,this paper deeply analyzes the transformation of teacher identity and the reconstruction of student abilities in the AI-PBL fusion scenario.AI not only reshapes the logic of cultivating students’abilities but also prompts teachers to achieve deep changes in their roles at the cognitive,relational,and ethical levels.Human-machine collaboration should not replace teachers’emotional values and educational judgments,but should become a key support for optimizing the educational ecology and realizing personalized education.展开更多
Objective:To assess the teaching efficacy of clinical instructors for MNS postgraduates,this study aims to gather insights into clinical educators’perceptions concerning MNS postgraduate training and identify key com...Objective:To assess the teaching efficacy of clinical instructors for MNS postgraduates,this study aims to gather insights into clinical educators’perceptions concerning MNS postgraduate training and identify key competencies required for effective teaching.Methods:This study utilized a cross-sectional design.An online questionnaire survey was distributed among clinical faculty members engaged in the education of MNS postgraduates.The survey addressed 4 areas:general teaching conditions,perceptions regarding training for MNS postgraduates,core competencies expected from clinical teachers,and implementation strategies for delivering clinical instruction tailored to these graduates.Results:A total of 143 clinical teachers supervising MNS postgraduates completed the questionnaire.Of these respondents,101(70.62%)held a bachelor’s degree while 42(29.37%)possessed a master’s degree.Although these clinicians demonstrated certain core competencies,they exhibited lower proficiency in understanding trends related to scientific advancements and current research priorities along with limited research capabilities 4.00(3.00,4.00).Notably,>95.8%of teachers recognized differences between the 2 categories of postgraduate students;only 4.2%were completely unaware of such distinctions.There was no statistically significant relationship between the demographic data of clinical educators and their core competencies(P>0.05).Conclusions:This study revealed no significant correlation between the general characteristics of clinical teachers and the core competencies of MNS postgraduates.Additionally,we identified a lack of effective communication and collaboration between MNS postgraduate supervisors and clinical educators.The findings from this research offer valuable insights for further investigation into the factors that influence the core competencies of clinical teachers,as well as strategies to enhance the clinical teaching standards for MNS postgraduates.展开更多
Background: Sickle cell anemia(SCA), a genetic hemoglobin disorder, suggests essential inner ear compromise and poor auditory processing. In humans, auditory processing differs physiologically between males and female...Background: Sickle cell anemia(SCA), a genetic hemoglobin disorder, suggests essential inner ear compromise and poor auditory processing. In humans, auditory processing differs physiologically between males and females, possibly true for SCA due to gender-specific disease pathophysiological changes. Objective: To investigate gender differences in psychoacoustical abilities, and speech perception in noise in SCA individuals and further compare with normal healthy(NH) population. Methods: 80 SCA and 80 NH normal-hearing participants aged 15-40 years were included and further grouped based on gender. Auditory discrimination for frequency, intensity, and duration at 500Hz and 4000Hz;temporal processing(Gap detection threshold & Modulation Detection Threshold) and Speech Perception In Noise(SPIN) at 0d BSNR tests were evaluated and compared between males and females of SCA and NH population. Results: SCA performed poorer compared to NH for all experimental measures. In the NH population, males performed poorer than females in psychoacoustical measures whereas within the SCA population, the reverse was true. Female participants performed better in the SPIN test in both populations. Conclusions: The adverse impact of SCA on the auditory system due to circulatory changes might cause poorer performance in SCA. Poorer performance by Female SCA is possibly due to the contrary impact of lower Hb level overlying Sickle disease.Estrogen levels and gender preference in auditory processing might lead to better performance by females within the NH population. SPIN performance depends on different attentional demands and sensorimotor processing strategies in noise beyond psychoacoustical processing may lead to better female performance in both populations.展开更多
Rheumatoid arthritis(RA)patients face significant psychological challenges alongside physical symptoms,necessitating a comprehensive understanding of how psychological vulnerability and adaptation patterns evolve thro...Rheumatoid arthritis(RA)patients face significant psychological challenges alongside physical symptoms,necessitating a comprehensive understanding of how psychological vulnerability and adaptation patterns evolve throughout the disease course.This review examined 95 studies(2000-2025)from PubMed,Web of Science,and CNKI databases including longitudinal cohorts,randomized controlled trials,and mixed-methods research,to characterize the complex interplay between biological,psychological,and social factors affecting RA patients’mental health.Findings revealed three distinct vulnerability trajectories(45%persistently low,30%fluctuating improvement,25%persistently high)and four adaptation stages,with critical intervention periods occurring 3-6 months postdiagnosis and during disease flares.Multiple factors significantly influence psychological outcomes,including gender(females showing 1.8-fold increased risk),age(younger patients experiencing 42%higher vulnerability),pain intensity,inflammatory markers,and neuroendocrine dysregulation(48%showing cortisol rhythm disruption).Early psychological intervention(within 3 months of diagnosis)demonstrated robust benefits,reducing depression incidence by 42%with effects persisting 24-36 months,while different modalities showed complementary advantages:Cognitive behavioral therapy for depression(Cohen’s d=0.68),mindfulness for pain acceptance(38%improvement),and peer support for meaning reconstruction(25.6%increase).These findings underscore the importance of integrating routine psychological assessment into standard RA care,developing stage-appropriate interventions,and advancing research toward personalized biopsychosocial approaches that address the dynamic psychological dimensions of the disease.展开更多
The transmitting of Chinese culture in the intercultural communication is of great importance for English learners in China.English majors at newly upgraded universities are weak in their abilities to express Chinese ...The transmitting of Chinese culture in the intercultural communication is of great importance for English learners in China.English majors at newly upgraded universities are weak in their abilities to express Chinese culture in English and transmit Chinese culture.To find measures for the problems,some necessary changes in the syllabus,textbooks,testing and curriculum are suggested,compiling of a dictionary for the standard of Chinese culture items in English is discussed,and the roles of teachers,students and English Corner in the cultivation of the abilities of English majors' to express Chinese culture in English at newly upgraded universities are analyzed.展开更多
Ti and Ag powders were mixed with different ball milling time （1, 2, 5 and 10 h） and sintered into porous Ti-3Ag alloys. The samples were treated with hydrothermal treatment, and their apatite-inducing abilities wer...Ti and Ag powders were mixed with different ball milling time （1, 2, 5 and 10 h） and sintered into porous Ti-3Ag alloys. The samples were treated with hydrothermal treatment, and their apatite-inducing abilities were further evaluated by immersion in modified simulated body fluid. The results indicate that the high surface energy brought by powder refinement leads to the decline of Ag, but promotes the oxidation of Ti during the sintering process. Meanwhile, the hydrothermal treated porous Ti-3Ag alloys prepared by the powders ball milled for 10 h possess the best apatite-inducing ability.展开更多
To select maternal parents with high fertility and high combining ability during cross breeding of Populus tomentosa Carr., the ploidy level of 75 female clones was determined using flow cytometry. In addition, geneti...To select maternal parents with high fertility and high combining ability during cross breeding of Populus tomentosa Carr., the ploidy level of 75 female clones was determined using flow cytometry. In addition, genetic variations and phenotypic correlations of seed traits and early growth traits, as well as the general combining ability(GCA) for seedling height(SH) and basal diameter(BD) of all diploid half-sib families were examined. A total of 26 natural triploid clones were identified. For all diploid families, family effects were significant for inflorescence length(IL), thousand kernel weight(TKW), seedling emergence rate(SER), and for SH and BD. There was a positive correlation between TKW and other traits,excluding IL. The seed-bearing coefficient(NS 9 SER)ranged from zero to 32.4%. Clones 3-10-2, 2-8, 3119,3206, and 3-10-1 had the best performance of the GCA for SH and BD. Based on the fertility and GCA for SH and BD, clone 3-10-2 and clone 3119 could be used as female parents for controlled cross-breeding of P. tomentosa.展开更多
Glycinebetaine （Glybet） accumulation, photosynthetic efficiency and growth performance in indica rice cultivated under salt stress and extreme pH stress were investigated. Betaine aldehyde dehydrogenase （BADH） act...Glycinebetaine （Glybet） accumulation, photosynthetic efficiency and growth performance in indica rice cultivated under salt stress and extreme pH stress were investigated. Betaine aldehyde dehydrogenase （BADH） activity and Glybet accumulation in the seedlings of salt-tolerant and salt-sensitive rice varieties grown under saline and acidic conditions peaked after treatment for 72 h and 96 h, respectively, and were higher than those grown under neutral pH and alkaline salt stress. A positive correlation was found between BADH activity and Glybet content in both salt-tolerant （P=0.71） and salt-sensitive （P=0.86） genotypes. The chlorophyll a, chlorophyll b, total chlorophyll and total carotenoids contents in the stressed seedlings significantly decreased under both acidic and alkaline stresses, especially in the salt-sensitive genotype. Similarly, the maximum quantum yield of PSII （Fv/Fm）, photon yield of PSII （ФPSII）, non-photochemical quenching （NPQ） and net photosynthetic rate （Pn） in the stressed seedlings were inhibited, leading to overall growth reduction. The positive correlations between chlorophyll a content and Fv/Fm, total chlorophyll content and ФPSII, and Pn as well as Pn and leaf area in both salt-tolerant and salt-sensitive genotypes were found. Saline acidic and saline alkaline soils may play a key role affecting vegetative growth prior to the reproductive stage in rice plants.展开更多
General combining abilities （GCAs） are very important in utilization of heterosis in maize breeding. However, its genetic basis is unclear. In the present study, a set of 118 doubled haploid （DH） lines were induce...General combining abilities （GCAs） are very important in utilization of heterosis in maize breeding. However, its genetic basis is unclear. In the present study, a set of 118 doubled haploid （DH） lines were induced from F1 generations produced from the cross between the inbred line Zheng 58 and the inbred line W499 belonging to the Reid subgroup. Using the MaizeSNP50 BeadChip, a high-density genetic map was constructed based on the DH population which included 1 147 bin markers with an average interval length of 2.00 cM. Meanwhile, the DH population was crossed with three testers including W16-5, HD568, and W556, which belong to the Sipingtou subgroup. The GCAs of the ear height （EH）, the kernel moisture content （KMC）, the kernel ratio （KR）, and the yield per plant （YPP） were estimated using these hybrids in three environments. Combining the high-density genetic map and the GCAs, a total of 14 QTLs were detected for the GCAs of the four traits. Especially, one pleiotropic QTL was identified on chromosome 1 between the SNP SYN16067 and the SNP PZE-101169244 which was simultaneously associated with the GCAs of the EH, the KR, and the YPP. These QTLs pave the way for further dissecting the genetic architecture underlying GCAs of the traits, and they may be used to enhance GCAs of inbred lines under the fixed heterotic pattern ReidxSipingtou in China through a marker-assisted selection approach.展开更多
The two most important activities in maize breeding are the development of inbred lines with high values of general combining ability(GCA)and specific combining ability(SCA),and the identification of hybrids with high...The two most important activities in maize breeding are the development of inbred lines with high values of general combining ability(GCA)and specific combining ability(SCA),and the identification of hybrids with high yield potentials.Genomic selection(GS)is a promising genomic tool to perform selection on the untested breeding material based on the genomic estimated breeding values estimated from the genomic prediction(GP).In this study,GP analyses were carried out to estimate the performance of hybrids,GCA,and SCA for grain yield(GY)in three maize line-by-tester trials,where all the material was phenotyped in 10 to 11 multiple-location trials and genotyped with a mid-density molecular marker platform.Results showed that the prediction abilities for the performance of hybrids ranged from 0.59 to0.81 across all trials in the model including the additive effect of lines and testers.In the model including both additive and non-additive effects,the prediction abilities for the performance of hybrids were improved and ranged from 0.64 to 0.86 across all trials.The prediction abilities of the GCA for GY were low,ranging between-0.14 and 0.13 across all trials in the model including only inbred lines;the prediction abilities of the GCA for GY were improved and ranged from 0.49 to 0.55 across all trials in the model including both inbred lines and testers,while the prediction abilities of the SCA for GY were negative across all trials.The prediction abilities for GY between testers varied from-0.66 to 0.82;the performance of hybrids between testers is difficult to predict.GS offers the opportunity to predict the performance of new hybrids and the GCA of new inbred lines based on the molecular marker information,the total breeding cost could be reduced dramatically by phenotyping fewer multiple-location trials.展开更多
Standardized Ginkgo biloba leaf extract has been used in clinical trials for its beneficial effects on brain func- tions, particularly in dementia. Substantial experimental evidences indicated that Ginkgo biloba leaf ...Standardized Ginkgo biloba leaf extract has been used in clinical trials for its beneficial effects on brain func- tions, particularly in dementia. Substantial experimental evidences indicated that Ginkgo biloba leaf extract （EGB） protected neuronal cells from a variety of insults. We investigated the effect of EGB on cognitive ability and protein kinase B （PKB） activity in hippocampal neuronal cells of dementia model rats. Rats received an intra- peritoneal injection of D-galactose to induce dementia. Forty-eight Spraque-Dawley rats were randomly divided into six groups, including the control group, D-galactose group （Gal）, low-dose EGB group （EGB-L）, mid-dose EGB group （EGB-M）, high-dose EGB group （EGB-H） and treatment group. The EGB-L, EGB-M and EGB-H groups were administered with EGB and D-galactose simultaneously. Y-maze, cresyl violet staining, TUNEL assays and immunohistochemistry staining were performed to detect learning and memory abilities, morpho- logical changes in the hippocampus, neuronal apoptosis and the expressing level of phospho-PKB, respectively. Rats in the Gal group showed decreased abilities of learning and memory, and hippocampal pyramidal cell layer was damaged, while EGB administration improved learning and memory abilities. The Gal group exhibited many stained, condensed nuclei and micronuclei, either isolated or within the cytoplasm of cells （39.5 ± 1.4）. Apoptotic cells decreased in the groups of EGB-L （35.9±0.9）, EGB-M （16.8± 1.0） and EGB-H （10.1±0.8）, and there were statistical significances compared with the Gal group. Immunoreactivity of phospho-PKB was localized diffusely throughout the cytosol of cells in all groups, while the immunoreactivity of the Gal group was weak. EGB signifi- cantly attenuated learning and memory impairment in a dose-dependent manner, while it could decrease the nmber of TUNEL-positive cells, and increase the activity of PKB. Our results demonstrated that EGB attenuated memory impairment and cell apoptosis in galactose-induced dementia model rats by activating PKB.展开更多
Bilateral Cochlear implants (CIs) improved speech intelligibility, speech perception in background noise, and sound localization in quiet and noisy situations. However, it is unclear whether these advantages essential...Bilateral Cochlear implants (CIs) improved speech intelligibility, speech perception in background noise, and sound localization in quiet and noisy situations. However, it is unclear whether these advantages essentially result in binaural integration of acoustic stimuli from each ear. In this study, we investigated the effectiveness of binaural integration by bilateral CIs placement using binaural hearing tests and subjective auditory perceptual assessment. A 61-year-old bilateral CIs subject underwent the following four tests:the Japanese Hearing in Noise Test (HINT-J), the dichotic listening test (DLT), the Rapidly Alternating Speech Perception (RASP) test, and subjective auditory perceptual assessment. The HINT-J score was significantly higher for bilateral CIs than for a unilateral CI. However, DLT and the RASP test revealed contradictory results. Subjective auditory perceptual assessment revealed active and bright impressions for bilateral hearing, which were also noisy and strong compared with those for unilateral hearing. The results of this study revealed that bilateral CIs improved speech perception in background noise and an improved auditory impression, although the bilateral integration abilities were not improved. This was probably because the patient was required to combine information from the two ears into a single perception in DLT and the RASP test. More longitudinal data should be collected and analyzed in future studies to evaluate the long-term effects of bilateral CIs. Copyright ? 2016, PLA General Hospital Department of Otolaryngology Head and Neck Surgery. Production and hosting by Elsevier (Singapore) Pte Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).展开更多
The genetic analysis of soybean isoflavone content and its components were carried out based on the NC Ⅱ matingdesign in eight soybean varieties. The results showed that the isoflavone contents and its components of ...The genetic analysis of soybean isoflavone content and its components were carried out based on the NC Ⅱ matingdesign in eight soybean varieties. The results showed that the isoflavone contents and its components of soybean seedare quite differences among the tested materials, the contents of isoflavone and daidzein are controlled not only byadditive effects and but also by non-additive effects, while the content of genistin is dominated by non-additive effects,and genistein, glycitin and daidzin are mainly controlled by additive effects. There are significant differences in thecontents of isoflavone and its components among the combinations derived from different parents. Results also indicatedthat the tested traits are negatively heterosis except for the contens of daidzein and daidzin are positively heterosis basedon the data of the GCA and SCA in average heterosis values. In this research we have a suggestion that soybean varietywith high isoflavone should be used as one of the parents in the breeding program, and it is the best choice that thecombinations crossed between two high isoflavone varieties or a high variety and a low one.展开更多
In a number of species,males and females have different ecological roles and therefore might be required to solve different problems.Studies on humans have suggested that the 2 sexes often show different efficiencies ...In a number of species,males and females have different ecological roles and therefore might be required to solve different problems.Studies on humans have suggested that the 2 sexes often show different efficiencies in problem solving tasks;similarly,evidence of sex differences has been found in 2 other mammalian species.Here,we assessed whether a teleost fish species,the guppy.Poecilia reticulata,displays sex differences in the ability to solve problems.In Experiment 1,guppies had to learn to dislodge a disc that occluded a feeder from which they had been previously accustomed to feed.In Experiment 2,guppies had to solve a version of the detour task that required them to learn to enter a transparent cylinder from the open sides to reach a food reward previously freely available.We found evidence of sex differences in both problem-solvingi tasks.In Experiment 1,females clearly outperformed males,and in Experiment 2,guppies showed a reversed but smaller sex difference.This study indicates that sex differences may play an important role in fish's problem-solving similar to what has previously been observed in some mammalian species.展开更多
In a number of animal species,individuals differ in their ability to solve cognitive tasks.However,the mechanisms underlying this variability remain unclear.It has been proposed that individual differences in cognitio...In a number of animal species,individuals differ in their ability to solve cognitive tasks.However,the mechanisms underlying this variability remain unclear.It has been proposed that individual differences in cognition may be related to individual differences in behavior(i.e.,personality);a hypothesis that has received mixed support.In this study,we investigated whether personality correlates with the cognitive ability that allows inhibiting behavior in 2 teleost fish species,the zebrafish Danio rerio and the guppy Poecilia reticulata.In both species,individuals that were bolder in a standard personality assay,the open-field test,showed greater inhibitory abilities in the tube task,which required them to inhibit foraging behavior toward live prey sealed into a transparent tube.This finding reveals a relationship between boldness and inhibitory abilities in fish and lends support to the hypothesis of a link between personality and cognition.Moreover,this study suggests that species separated by a relatively large phylogenetic distance may show the same link between personality and cognition,when tested on the same tasks.展开更多
Geographical background and dispersal ability may strongly influence assemblage dissimilarity;however,these aspects have generally been overlooked in previous large-scale beta diversity studies.Here,we examined whethe...Geographical background and dispersal ability may strongly influence assemblage dissimilarity;however,these aspects have generally been overlooked in previous large-scale beta diversity studies.Here,we examined whether the patterns and drivers of taxonomic beta diversity(TBD)and phylogenetic beta diversity(PBD)of breeding birds in China vary across(1)regions on both sides of the Hu Line,which demarcates China’s topographical,climatic,economic,and social patterns,and(2)species with different dispersal ability.TBD and PBD were calculated and partitioned into turnover and nestedness components using a moving window approach.Variables representing climate,habitat heterogeneity,and habitat quality were employed to evaluate the effects of environmental filtering.Spatial distance was considered to assess the impact of dispersal limitation.Variance partitioning analysis was applied to assess the relative roles of these variables.In general,the values of TBD and PBD were high in mountainous areas and were largely determined by environmental filtering.However,different dominant environmental filters on either side of the Hu Line led to divergent beta diversity patterns.Specifically,climate-driven species turnover and habitat heterogeneity-related species nestedness dominated the regions east and west of the line,respectively.Additionally,bird species with stronger dispersal ability were more susceptible to environmental filtering,resulting in more homogeneous assemblages.Our results indicated that regions with distinctive geographical backgrounds may present different ecological factors that lead to divergent assemblage dissimilarity patterns,and dispersal ability determines the response of assemblages to these ecological factors.Identifying a single universal explanation for the observed pattern without considering these aspects may lead to simplistic or incomplete conclusions.Consequently,a comprehensive understanding of large-scale beta diversity patterns and effective planning of conservation strategies necessitate the consideration of both geographical background and species dispersal ability.展开更多
Purpose:This study compared the relative effectiveness of Chinese calligraphy handwriting(CCH)and acupuncture in the treatment of patients with senile dementia.Materials and Methods:A randomized controlled trial(RCT)w...Purpose:This study compared the relative effectiveness of Chinese calligraphy handwriting(CCH)and acupuncture in the treatment of patients with senile dementia.Materials and Methods:A randomized controlled trial(RCT)with 17 mild-to-moderate dementia patients with an average age of 77.29 years were randomly assigned with 9 to the calligraphy handwriting group and 8 to the acupuncture treatment group for a month of consecutive treatment.The participants'cognitive abilities,as well as symptoms of senile dementia,were measured by the Chinese version of the Mini-mental State Examination(CMMSE)and the Chinese Medicine Quantitative Diagnostic Survey for Senile Dementia Symptoms,respectively,before and after the treatment.Results:The calligraphy group showed a significant increase in calculation and memory as well as a decline in the symptoms of senile dementia.Patients in the acupuncture group experienced a significant growth in total CMMSE scores and the subscales in orientation to time and place,behavioral operations,as well as reduced clinical symptoms.However,no significant changes were found in their memory and calculation abilities.Conclusion:Both CCH and acupuncture treatments were found significantly effective for,respectively,enhancing the patients'cognitive abilities and reducing their clinical symptoms.Further,calligraphy handwriting also improved the level of their attention and concentration,physical relaxation,and emotional stability.展开更多
Thinking ability of a man, which is the core of wisdom, participates in and dominates all the intellectual activities. This study, pursued on the ontological and practical level, is aimed at cultivating learners '...Thinking ability of a man, which is the core of wisdom, participates in and dominates all the intellectual activities. This study, pursued on the ontological and practical level, is aimed at cultivating learners ' thinking abilities in foreign language teaching(FLT)in basic education of China. Regarding English teaching reform in basic education of China as the background, this thesis probes to bring it to the forefront and put forward approaches and strategies of incorporating the cultivation of learners' thinking abilities into every aspect of the teaching procedures.展开更多
基金founded by the National Key R&D Program of China(No.2022YFD2400305)the Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province(Nos.2022LZGCQY010,2021LZGC027 and 2021ZLGX03)the China Agriculture Research System Project(No.CARS-49)。
文摘A crossbreeding program was established in 2019 to address the declining Crassostrea gigas harvests caused by Pacific Oyster Mortality Syndrome(POMS).As a part of the program,this study was performed to estimate the genetic structure underlying phenotypic variation.Fifteen complete diallel crossings of C.gigas and C.angulata,comprising 60 full-sib families,were used to evaluate the general combining ability(GCA)and specific combining ability(SCA),as well as genotype and environment interactions for shell height(SH),summer survival(SS),and thermal tolerance(TT)of reciprocal hybrids GA(C.gigas♀×C.angulata♂)and AG(C.angulata♀×C.gigas♂)grown in Rongcheng and Rushan,Shandong Province,China.The results suggested that heterosis of the reciprocal hybrids was evident for SH,SS,and TT.The hybrid GA had larger heterosis than AG in both testing environments,and can be a potential donor in the breeding program.The male C.gigas had better GCA for SH in Rongcheng,whereas male C.angulata was a good general combiner for SS and TT in both Rongcheng and Rushan.The estimate of SCA was much higher than GCA for SH and lower than GCA for TT.To harness both additive and non-additive genetic effects,combination breed-ing could be taken to develop hybrid varieties possessing both thermal tolerance and fast-growing traits.The positive correlations between SH and TT suggested that these traits could be improved simultaneously.The significant G×E interactions demonstrated the importance to undertake site-specific breeding programs in different environments.Overall,this study can provide essential information for developing crossbreeding strategies for the oyster farming industry.
基金The 2023 Jilin Provincial Department of Education Science and Technology(Social Sciences)Research Planning Project“Research on the Organizational Models of Private Universities in Promoting Rural Revital-ization in Jilin Province”(JJKH20231426SK)。
文摘This paper focuses on the transformation of the project-based learning(PBL)teaching model driven by artificial intelligence(AI),and explores the two-way reconstruction path of teacher roles and student abilities.Combining metacognitive theory to analyze the pain points of traditional PBL,this paper systematically sorts out the functional reconstruction path of AI in the dimensions of teaching design,process monitoring,and evaluation feedback.Then,starting from the social role theory,this paper deeply analyzes the transformation of teacher identity and the reconstruction of student abilities in the AI-PBL fusion scenario.AI not only reshapes the logic of cultivating students’abilities but also prompts teachers to achieve deep changes in their roles at the cognitive,relational,and ethical levels.Human-machine collaboration should not replace teachers’emotional values and educational judgments,but should become a key support for optimizing the educational ecology and realizing personalized education.
基金supported by the Medical Education Research Initiative of Henan Province,grant funded by the Henan Provincial Health Commission(No.Wjlx2022186).
文摘Objective:To assess the teaching efficacy of clinical instructors for MNS postgraduates,this study aims to gather insights into clinical educators’perceptions concerning MNS postgraduate training and identify key competencies required for effective teaching.Methods:This study utilized a cross-sectional design.An online questionnaire survey was distributed among clinical faculty members engaged in the education of MNS postgraduates.The survey addressed 4 areas:general teaching conditions,perceptions regarding training for MNS postgraduates,core competencies expected from clinical teachers,and implementation strategies for delivering clinical instruction tailored to these graduates.Results:A total of 143 clinical teachers supervising MNS postgraduates completed the questionnaire.Of these respondents,101(70.62%)held a bachelor’s degree while 42(29.37%)possessed a master’s degree.Although these clinicians demonstrated certain core competencies,they exhibited lower proficiency in understanding trends related to scientific advancements and current research priorities along with limited research capabilities 4.00(3.00,4.00).Notably,>95.8%of teachers recognized differences between the 2 categories of postgraduate students;only 4.2%were completely unaware of such distinctions.There was no statistically significant relationship between the demographic data of clinical educators and their core competencies(P>0.05).Conclusions:This study revealed no significant correlation between the general characteristics of clinical teachers and the core competencies of MNS postgraduates.Additionally,we identified a lack of effective communication and collaboration between MNS postgraduate supervisors and clinical educators.The findings from this research offer valuable insights for further investigation into the factors that influence the core competencies of clinical teachers,as well as strategies to enhance the clinical teaching standards for MNS postgraduates.
文摘Background: Sickle cell anemia(SCA), a genetic hemoglobin disorder, suggests essential inner ear compromise and poor auditory processing. In humans, auditory processing differs physiologically between males and females, possibly true for SCA due to gender-specific disease pathophysiological changes. Objective: To investigate gender differences in psychoacoustical abilities, and speech perception in noise in SCA individuals and further compare with normal healthy(NH) population. Methods: 80 SCA and 80 NH normal-hearing participants aged 15-40 years were included and further grouped based on gender. Auditory discrimination for frequency, intensity, and duration at 500Hz and 4000Hz;temporal processing(Gap detection threshold & Modulation Detection Threshold) and Speech Perception In Noise(SPIN) at 0d BSNR tests were evaluated and compared between males and females of SCA and NH population. Results: SCA performed poorer compared to NH for all experimental measures. In the NH population, males performed poorer than females in psychoacoustical measures whereas within the SCA population, the reverse was true. Female participants performed better in the SPIN test in both populations. Conclusions: The adverse impact of SCA on the auditory system due to circulatory changes might cause poorer performance in SCA. Poorer performance by Female SCA is possibly due to the contrary impact of lower Hb level overlying Sickle disease.Estrogen levels and gender preference in auditory processing might lead to better performance by females within the NH population. SPIN performance depends on different attentional demands and sensorimotor processing strategies in noise beyond psychoacoustical processing may lead to better female performance in both populations.
基金Supported by Chongqing Health Commission and Chongqing Science and Technology Bureau,No.2023MSXM182。
文摘Rheumatoid arthritis(RA)patients face significant psychological challenges alongside physical symptoms,necessitating a comprehensive understanding of how psychological vulnerability and adaptation patterns evolve throughout the disease course.This review examined 95 studies(2000-2025)from PubMed,Web of Science,and CNKI databases including longitudinal cohorts,randomized controlled trials,and mixed-methods research,to characterize the complex interplay between biological,psychological,and social factors affecting RA patients’mental health.Findings revealed three distinct vulnerability trajectories(45%persistently low,30%fluctuating improvement,25%persistently high)and four adaptation stages,with critical intervention periods occurring 3-6 months postdiagnosis and during disease flares.Multiple factors significantly influence psychological outcomes,including gender(females showing 1.8-fold increased risk),age(younger patients experiencing 42%higher vulnerability),pain intensity,inflammatory markers,and neuroendocrine dysregulation(48%showing cortisol rhythm disruption).Early psychological intervention(within 3 months of diagnosis)demonstrated robust benefits,reducing depression incidence by 42%with effects persisting 24-36 months,while different modalities showed complementary advantages:Cognitive behavioral therapy for depression(Cohen’s d=0.68),mindfulness for pain acceptance(38%improvement),and peer support for meaning reconstruction(25.6%increase).These findings underscore the importance of integrating routine psychological assessment into standard RA care,developing stage-appropriate interventions,and advancing research toward personalized biopsychosocial approaches that address the dynamic psychological dimensions of the disease.
文摘The transmitting of Chinese culture in the intercultural communication is of great importance for English learners in China.English majors at newly upgraded universities are weak in their abilities to express Chinese culture in English and transmit Chinese culture.To find measures for the problems,some necessary changes in the syllabus,textbooks,testing and curriculum are suggested,compiling of a dictionary for the standard of Chinese culture items in English is discussed,and the roles of teachers,students and English Corner in the cultivation of the abilities of English majors' to express Chinese culture in English at newly upgraded universities are analyzed.
基金Projects(2012CB619102,2012CB619100)supported by the National Basic Research Program of ChinaProjects(2011AA030101,2011AA030103)supported by the High-tech Research and Development Program of China+1 种基金Projects(HEUCFZ1017,HEUCFR1020)supported by the Fundamental Research Funds for the Central Universities,ChinaProject(ZD201012)supported by the Natural Science Foundation of Heilongjiang Province,China
文摘Ti and Ag powders were mixed with different ball milling time （1, 2, 5 and 10 h） and sintered into porous Ti-3Ag alloys. The samples were treated with hydrothermal treatment, and their apatite-inducing abilities were further evaluated by immersion in modified simulated body fluid. The results indicate that the high surface energy brought by powder refinement leads to the decline of Ag, but promotes the oxidation of Ti during the sintering process. Meanwhile, the hydrothermal treated porous Ti-3Ag alloys prepared by the powders ball milled for 10 h possess the best apatite-inducing ability.
基金supported by the Special Fund for Beijing Common Construction Project
文摘To select maternal parents with high fertility and high combining ability during cross breeding of Populus tomentosa Carr., the ploidy level of 75 female clones was determined using flow cytometry. In addition, genetic variations and phenotypic correlations of seed traits and early growth traits, as well as the general combining ability(GCA) for seedling height(SH) and basal diameter(BD) of all diploid half-sib families were examined. A total of 26 natural triploid clones were identified. For all diploid families, family effects were significant for inflorescence length(IL), thousand kernel weight(TKW), seedling emergence rate(SER), and for SH and BD. There was a positive correlation between TKW and other traits,excluding IL. The seed-bearing coefficient(NS 9 SER)ranged from zero to 32.4%. Clones 3-10-2, 2-8, 3119,3206, and 3-10-1 had the best performance of the GCA for SH and BD. Based on the fertility and GCA for SH and BD, clone 3-10-2 and clone 3119 could be used as female parents for controlled cross-breeding of P. tomentosa.
基金supported by the National Center for Genetic Engineering and Biotechnology (BIOTEC) (Grant No. BT-B-06-RG-14-4502)partly funded by the International Atomic Energy Agency (IAEA) (Contract No. 12998/R0)
文摘Glycinebetaine （Glybet） accumulation, photosynthetic efficiency and growth performance in indica rice cultivated under salt stress and extreme pH stress were investigated. Betaine aldehyde dehydrogenase （BADH） activity and Glybet accumulation in the seedlings of salt-tolerant and salt-sensitive rice varieties grown under saline and acidic conditions peaked after treatment for 72 h and 96 h, respectively, and were higher than those grown under neutral pH and alkaline salt stress. A positive correlation was found between BADH activity and Glybet content in both salt-tolerant （P=0.71） and salt-sensitive （P=0.86） genotypes. The chlorophyll a, chlorophyll b, total chlorophyll and total carotenoids contents in the stressed seedlings significantly decreased under both acidic and alkaline stresses, especially in the salt-sensitive genotype. Similarly, the maximum quantum yield of PSII （Fv/Fm）, photon yield of PSII （ФPSII）, non-photochemical quenching （NPQ） and net photosynthetic rate （Pn） in the stressed seedlings were inhibited, leading to overall growth reduction. The positive correlations between chlorophyll a content and Fv/Fm, total chlorophyll content and ФPSII, and Pn as well as Pn and leaf area in both salt-tolerant and salt-sensitive genotypes were found. Saline acidic and saline alkaline soils may play a key role affecting vegetative growth prior to the reproductive stage in rice plants.
基金financially supported by the National Key Research and Development Plan of China(2016YFD0101200)
文摘General combining abilities （GCAs） are very important in utilization of heterosis in maize breeding. However, its genetic basis is unclear. In the present study, a set of 118 doubled haploid （DH） lines were induced from F1 generations produced from the cross between the inbred line Zheng 58 and the inbred line W499 belonging to the Reid subgroup. Using the MaizeSNP50 BeadChip, a high-density genetic map was constructed based on the DH population which included 1 147 bin markers with an average interval length of 2.00 cM. Meanwhile, the DH population was crossed with three testers including W16-5, HD568, and W556, which belong to the Sipingtou subgroup. The GCAs of the ear height （EH）, the kernel moisture content （KMC）, the kernel ratio （KR）, and the yield per plant （YPP） were estimated using these hybrids in three environments. Combining the high-density genetic map and the GCAs, a total of 14 QTLs were detected for the GCAs of the four traits. Especially, one pleiotropic QTL was identified on chromosome 1 between the SNP SYN16067 and the SNP PZE-101169244 which was simultaneously associated with the GCAs of the EH, the KR, and the YPP. These QTLs pave the way for further dissecting the genetic architecture underlying GCAs of the traits, and they may be used to enhance GCAs of inbred lines under the fixed heterotic pattern ReidxSipingtou in China through a marker-assisted selection approach.
基金financial support of the Shanghai Agriculture Applied Technology Development Program of China(Z20190101)the Harvest Plus Project+7 种基金the Genomic Opensource Breeding Informatics Initiative(GOBII)(OPP1093167)supported by the Bill&Melinda Gates Foundationthe CGIAR Research Program(CRP)on MAIZEW1&W2 support from the Governments of Australia,Belgium,Canada,China,France,India,Japan,Republic of Korea,Mexico,the Netherlands,New Zealand,Norway,Sweden,Switzerland,the United Kingdom,the United States,and the World Bankgrants from the National Key Research and Development Program of China(2016YFD0101803)the National Natural Science Foundation of China(31801442)Shenyang City Key Laboratory of Maize Genomic Selection,Liaoning Province Key Scientific and Technological Research and Development Project(2011208001)the CIMMYT-China Specialty Maize Research Center Project funded by the Shanghai Municipal Finance Bureau(KF201802)the Chinese Scholarship Council。
文摘The two most important activities in maize breeding are the development of inbred lines with high values of general combining ability(GCA)and specific combining ability(SCA),and the identification of hybrids with high yield potentials.Genomic selection(GS)is a promising genomic tool to perform selection on the untested breeding material based on the genomic estimated breeding values estimated from the genomic prediction(GP).In this study,GP analyses were carried out to estimate the performance of hybrids,GCA,and SCA for grain yield(GY)in three maize line-by-tester trials,where all the material was phenotyped in 10 to 11 multiple-location trials and genotyped with a mid-density molecular marker platform.Results showed that the prediction abilities for the performance of hybrids ranged from 0.59 to0.81 across all trials in the model including the additive effect of lines and testers.In the model including both additive and non-additive effects,the prediction abilities for the performance of hybrids were improved and ranged from 0.64 to 0.86 across all trials.The prediction abilities of the GCA for GY were low,ranging between-0.14 and 0.13 across all trials in the model including only inbred lines;the prediction abilities of the GCA for GY were improved and ranged from 0.49 to 0.55 across all trials in the model including both inbred lines and testers,while the prediction abilities of the SCA for GY were negative across all trials.The prediction abilities for GY between testers varied from-0.66 to 0.82;the performance of hybrids between testers is difficult to predict.GS offers the opportunity to predict the performance of new hybrids and the GCA of new inbred lines based on the molecular marker information,the total breeding cost could be reduced dramatically by phenotyping fewer multiple-location trials.
文摘Standardized Ginkgo biloba leaf extract has been used in clinical trials for its beneficial effects on brain func- tions, particularly in dementia. Substantial experimental evidences indicated that Ginkgo biloba leaf extract （EGB） protected neuronal cells from a variety of insults. We investigated the effect of EGB on cognitive ability and protein kinase B （PKB） activity in hippocampal neuronal cells of dementia model rats. Rats received an intra- peritoneal injection of D-galactose to induce dementia. Forty-eight Spraque-Dawley rats were randomly divided into six groups, including the control group, D-galactose group （Gal）, low-dose EGB group （EGB-L）, mid-dose EGB group （EGB-M）, high-dose EGB group （EGB-H） and treatment group. The EGB-L, EGB-M and EGB-H groups were administered with EGB and D-galactose simultaneously. Y-maze, cresyl violet staining, TUNEL assays and immunohistochemistry staining were performed to detect learning and memory abilities, morpho- logical changes in the hippocampus, neuronal apoptosis and the expressing level of phospho-PKB, respectively. Rats in the Gal group showed decreased abilities of learning and memory, and hippocampal pyramidal cell layer was damaged, while EGB administration improved learning and memory abilities. The Gal group exhibited many stained, condensed nuclei and micronuclei, either isolated or within the cytoplasm of cells （39.5 ± 1.4）. Apoptotic cells decreased in the groups of EGB-L （35.9±0.9）, EGB-M （16.8± 1.0） and EGB-H （10.1±0.8）, and there were statistical significances compared with the Gal group. Immunoreactivity of phospho-PKB was localized diffusely throughout the cytosol of cells in all groups, while the immunoreactivity of the Gal group was weak. EGB signifi- cantly attenuated learning and memory impairment in a dose-dependent manner, while it could decrease the nmber of TUNEL-positive cells, and increase the activity of PKB. Our results demonstrated that EGB attenuated memory impairment and cell apoptosis in galactose-induced dementia model rats by activating PKB.
文摘Bilateral Cochlear implants (CIs) improved speech intelligibility, speech perception in background noise, and sound localization in quiet and noisy situations. However, it is unclear whether these advantages essentially result in binaural integration of acoustic stimuli from each ear. In this study, we investigated the effectiveness of binaural integration by bilateral CIs placement using binaural hearing tests and subjective auditory perceptual assessment. A 61-year-old bilateral CIs subject underwent the following four tests:the Japanese Hearing in Noise Test (HINT-J), the dichotic listening test (DLT), the Rapidly Alternating Speech Perception (RASP) test, and subjective auditory perceptual assessment. The HINT-J score was significantly higher for bilateral CIs than for a unilateral CI. However, DLT and the RASP test revealed contradictory results. Subjective auditory perceptual assessment revealed active and bright impressions for bilateral hearing, which were also noisy and strong compared with those for unilateral hearing. The results of this study revealed that bilateral CIs improved speech perception in background noise and an improved auditory impression, although the bilateral integration abilities were not improved. This was probably because the patient was required to combine information from the two ears into a single perception in DLT and the RASP test. More longitudinal data should be collected and analyzed in future studies to evaluate the long-term effects of bilateral CIs. Copyright ? 2016, PLA General Hospital Department of Otolaryngology Head and Neck Surgery. Production and hosting by Elsevier (Singapore) Pte Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
基金This work was jointly spored by the grants from the Natural Science Foundation of Henan Province(0511030500)the National 863 Program of China(2004AA2111112)the N ational Natural Science Foundation of China(30490250).
文摘The genetic analysis of soybean isoflavone content and its components were carried out based on the NC Ⅱ matingdesign in eight soybean varieties. The results showed that the isoflavone contents and its components of soybean seedare quite differences among the tested materials, the contents of isoflavone and daidzein are controlled not only byadditive effects and but also by non-additive effects, while the content of genistin is dominated by non-additive effects,and genistein, glycitin and daidzin are mainly controlled by additive effects. There are significant differences in thecontents of isoflavone and its components among the combinations derived from different parents. Results also indicatedthat the tested traits are negatively heterosis except for the contens of daidzein and daidzin are positively heterosis basedon the data of the GCA and SCA in average heterosis values. In this research we have a suggestion that soybean varietywith high isoflavone should be used as one of the parents in the breeding program, and it is the best choice that thecombinations crossed between two high isoflavone varieties or a high variety and a low one.
基金Funding was provided by PRIN 2015 Grant(prot.:2015FFATB7)to A.B.from Ministero dell'Istruzione,Universita e Ricerca(M IUR,Italy)FIR2018 and FAR2018 grants to T.L.X.from University of Ferrara.
文摘In a number of species,males and females have different ecological roles and therefore might be required to solve different problems.Studies on humans have suggested that the 2 sexes often show different efficiencies in problem solving tasks;similarly,evidence of sex differences has been found in 2 other mammalian species.Here,we assessed whether a teleost fish species,the guppy.Poecilia reticulata,displays sex differences in the ability to solve problems.In Experiment 1,guppies had to learn to dislodge a disc that occluded a feeder from which they had been previously accustomed to feed.In Experiment 2,guppies had to solve a version of the detour task that required them to learn to enter a transparent cylinder from the open sides to reach a food reward previously freely available.We found evidence of sex differences in both problem-solvingi tasks.In Experiment 1,females clearly outperformed males,and in Experiment 2,guppies showed a reversed but smaller sex difference.This study indicates that sex differences may play an important role in fish's problem-solving similar to what has previously been observed in some mammalian species.
基金Funding was provided by FAR2018 and FIR2018 grant from University of Ferrara to T.L.-X.
文摘In a number of animal species,individuals differ in their ability to solve cognitive tasks.However,the mechanisms underlying this variability remain unclear.It has been proposed that individual differences in cognition may be related to individual differences in behavior(i.e.,personality);a hypothesis that has received mixed support.In this study,we investigated whether personality correlates with the cognitive ability that allows inhibiting behavior in 2 teleost fish species,the zebrafish Danio rerio and the guppy Poecilia reticulata.In both species,individuals that were bolder in a standard personality assay,the open-field test,showed greater inhibitory abilities in the tube task,which required them to inhibit foraging behavior toward live prey sealed into a transparent tube.This finding reveals a relationship between boldness and inhibitory abilities in fish and lends support to the hypothesis of a link between personality and cognition.Moreover,this study suggests that species separated by a relatively large phylogenetic distance may show the same link between personality and cognition,when tested on the same tasks.
基金supported by the National Natural Science Foundation of China(31901220)Science and Technology Planning Project of Guangdong Province,China(2019B121202004)+1 种基金Guangdong Basic and Applied Basic Research Foundation(2021A1515110744)Forestry Administration of Guangdong Province(DFGP Project of Fauna of Guangdong-202115)。
文摘Geographical background and dispersal ability may strongly influence assemblage dissimilarity;however,these aspects have generally been overlooked in previous large-scale beta diversity studies.Here,we examined whether the patterns and drivers of taxonomic beta diversity(TBD)and phylogenetic beta diversity(PBD)of breeding birds in China vary across(1)regions on both sides of the Hu Line,which demarcates China’s topographical,climatic,economic,and social patterns,and(2)species with different dispersal ability.TBD and PBD were calculated and partitioned into turnover and nestedness components using a moving window approach.Variables representing climate,habitat heterogeneity,and habitat quality were employed to evaluate the effects of environmental filtering.Spatial distance was considered to assess the impact of dispersal limitation.Variance partitioning analysis was applied to assess the relative roles of these variables.In general,the values of TBD and PBD were high in mountainous areas and were largely determined by environmental filtering.However,different dominant environmental filters on either side of the Hu Line led to divergent beta diversity patterns.Specifically,climate-driven species turnover and habitat heterogeneity-related species nestedness dominated the regions east and west of the line,respectively.Additionally,bird species with stronger dispersal ability were more susceptible to environmental filtering,resulting in more homogeneous assemblages.Our results indicated that regions with distinctive geographical backgrounds may present different ecological factors that lead to divergent assemblage dissimilarity patterns,and dispersal ability determines the response of assemblages to these ecological factors.Identifying a single universal explanation for the observed pattern without considering these aspects may lead to simplistic or incomplete conclusions.Consequently,a comprehensive understanding of large-scale beta diversity patterns and effective planning of conservation strategies necessitate the consideration of both geographical background and species dispersal ability.
文摘Purpose:This study compared the relative effectiveness of Chinese calligraphy handwriting(CCH)and acupuncture in the treatment of patients with senile dementia.Materials and Methods:A randomized controlled trial(RCT)with 17 mild-to-moderate dementia patients with an average age of 77.29 years were randomly assigned with 9 to the calligraphy handwriting group and 8 to the acupuncture treatment group for a month of consecutive treatment.The participants'cognitive abilities,as well as symptoms of senile dementia,were measured by the Chinese version of the Mini-mental State Examination(CMMSE)and the Chinese Medicine Quantitative Diagnostic Survey for Senile Dementia Symptoms,respectively,before and after the treatment.Results:The calligraphy group showed a significant increase in calculation and memory as well as a decline in the symptoms of senile dementia.Patients in the acupuncture group experienced a significant growth in total CMMSE scores and the subscales in orientation to time and place,behavioral operations,as well as reduced clinical symptoms.However,no significant changes were found in their memory and calculation abilities.Conclusion:Both CCH and acupuncture treatments were found significantly effective for,respectively,enhancing the patients'cognitive abilities and reducing their clinical symptoms.Further,calligraphy handwriting also improved the level of their attention and concentration,physical relaxation,and emotional stability.
文摘Thinking ability of a man, which is the core of wisdom, participates in and dominates all the intellectual activities. This study, pursued on the ontological and practical level, is aimed at cultivating learners ' thinking abilities in foreign language teaching(FLT)in basic education of China. Regarding English teaching reform in basic education of China as the background, this thesis probes to bring it to the forefront and put forward approaches and strategies of incorporating the cultivation of learners' thinking abilities into every aspect of the teaching procedures.
