The trade-off between leaf size and leafing intensity(i.e.,the number of leaves per unit stem size)is a key axis of trait covariation across the diversity of plant foliage deployment.However,the functional significanc...The trade-off between leaf size and leafing intensity(i.e.,the number of leaves per unit stem size)is a key axis of trait covariation across the diversity of plant foliage deployment.However,the functional significance of leafing intensity and its possible combinations with leaf size in dealing with water limitation remains unclear.Using Populus euphratica as an illustrative tree species growing in hyper-arid climates,we investigated how leaf size and leafing intensity co-varied under varying water stresses.In the Ebinor lowlands and the upper reaches of the Tarim River(NW China),we sampled>1800 current-year twigs from 505 trees across 14 sites along a climatic gradient characterized by precipitation,potential evapotranspiration and vapor pressure deficit.Leafing intensity based on stem mass(LIM)decreased with climatic aridity,primarily due to greater stem mass,but not fewer leaves.This indicates a higher investment in structural support for leaf attachment under water stress.Both leaf area and mass decreased with LIM at a lower-than-proportional rate,with the decrease in leaf size being more pronounced under drier climates.This suggests that higher LIM incurs a high cost of reducing leaf size in water-limited habitats.These findings challenge the assumption that higher leafing intensity always confers an advantage ready for environmental stresses due to higher developmental flexibility offered by more axillary buds.Rather,we propose that a strategy of lower leafing intensity,with greater structural support for leaf attachment and less compromise in leaf size,can be advantageous under water limitation.展开更多
The leafing rates of fourteen rice varieties were measured in a sowing-time experiment, and a rapid leafing genotype at the vegetative growth stage was discovered in an indica variety Yanhui 559. The leaf number on th...The leafing rates of fourteen rice varieties were measured in a sowing-time experiment, and a rapid leafing genotype at the vegetative growth stage was discovered in an indica variety Yanhui 559. The leaf number on the main culm of Yanhui 559 was always 4-5 leaves more than that of Lemont, and the leafing rate of Yanhui 559 was significantly higher than that of Lemont based on similar growth durations from sowing to heading. Furthermore, the difference of the leafing rate was significant at the vegetative growth stage, but not distinctive at the panicle initiation stage. Genetic analysis of the leafing rates in the two backcross populations of Yanhui 559 and Lemont showed that major and quantitative genes controlled the expression of rapid leafing character. Based on results of investigation for some plants with similar growth durations in the backcrossing populations, the rapid leafing genotypes exhibited earlier tillering and more tiller numbers per plant, and its yield components including the number of panicles per plant and number of grains per panicle were superior to those of the slow leafing genotypes. Further research and application feasibility of the rapid leafing genotype in breeding were discussed.展开更多
Anthocyanin-rich foliage plants hold important applications in the pharmaceutical industry and the tea sector,beyond their significant ornamental value.These plants also possess biological and ecological importance,co...Anthocyanin-rich foliage plants hold important applications in the pharmaceutical industry and the tea sector,beyond their significant ornamental value.These plants also possess biological and ecological importance,contributing to reproduction,defense against natural enemies,and adaptation to environmental changes.Thus,a deeper understanding of their leaf coloration will be essential for both practical applications and theoretical understanding.The present study comprehensively reviews the factors influencing anthocyanin metabolism,including biosynthesis,transport,degradation,transcription factors(TF_(S)),post-transcriptional regulation,post translation regulation.Next,we summarize the application of omics technologies in unveiling the mechanisms of anthocyanin synthesis in leaves.Furthermore,we review the molecular mechanisms by which environmental factors regulate leaf coloration by inducing anthocyanin biosynthesis.Lastly,the study addresses unresolved issues in the research of plant leaf coloration and proposes future research directions in this field.This study is anticipated to provide a valuable reference for the study of plant leaf coloration.展开更多
Leaf thickness in rice critically influences photosynthetic efficiency and yield,yet its genetic basis remains poorly understood,with few functional genes previously characterized.In this study,we employ a pangenome-w...Leaf thickness in rice critically influences photosynthetic efficiency and yield,yet its genetic basis remains poorly understood,with few functional genes previously characterized.In this study,we employ a pangenome-wide association study(Pan-GWAS)on 302 diverse rice accessions from southern China,identifying 49 quantitative trait loci(QTLs)associated with leaf thickness.The most significant locus,qLT9,is fine-mapped to a 79-kb region on chromosome 9.Transcriptomic and genomic sequence analyses identify LOC_Os09g33480,which encodes a protein belonging to Multiple Organellar RNA Editing Factor family,as the key candidate gene.Overexpression and complementation transgenic experiments confirm LOC_Os09g33480(OsLT9)as the functional gene underlying qLT9,demonstrating a 24-bp Indel in its promoter correlates with the expression levels and leaf thickness.Notably,OsLT9 overexpression lines show not only thicker leaf,but also significantly enhanced photosynthetic efficiency and grain yield,establishing a link between leaf thickness modulation and yield enhancement.Population genomic analyses indicate strong selection for OsLT9 during domestication and breeding,with modern cultivars favoring thick leaf haplotype of OsLT9.This study establishes OsLT9 as a key regulator controlling leaf thickness in rice,and provides a valuable genetic resource for molecular breeding of high-yielding rice through optimization of plant architecture.展开更多
This paper describes two new species in the genus Cneorella Medvedev&Dang,1981 from China:C.flava sp.nov.and C.hainanensis sp.nov.External morphological photographs and illustrations of the male genitalia of these...This paper describes two new species in the genus Cneorella Medvedev&Dang,1981 from China:C.flava sp.nov.and C.hainanensis sp.nov.External morphological photographs and illustrations of the male genitalia of these new species are provided.A key to the Chinese known species in this genus is given.展开更多
Leaf angle is a pivotal agronomic trait that significantly influences crop architecture and yield.Plant hormones,such as auxin,play a critical role in regulating leaf angle;however,the underlying molecular mechanisms ...Leaf angle is a pivotal agronomic trait that significantly influences crop architecture and yield.Plant hormones,such as auxin,play a critical role in regulating leaf angle;however,the underlying molecular mechanisms remain to be fully elucidated.Here,we reveal that the auxin response factor gene,OsARF12,which is highly expressed in the leaf lamina joint,negatively regulates rice(Oryza sativa L.)leaf angle via affecting shoot gravitropism.Overexpression lines of OsARF12 exhibit more erect leaf angles,while the osarf12 mutants display enlarged leaf angles compared to the wild type.Further studies demonstrate that OsARF12 directly activates the expression of Loose Plant Architecture1(LPA1)and LAZY1 by binding to their promoters.The osarf12 mutant presents impaired shoot gravitropism,a phenotype consistent with that of the lpa1 and lazy1 mutants.Collectively,we elucidate the biological functions of OsARF12,which modulates leaf angle through its impact on shoot gravitropism by regulating the expression levels of LPA1 and LAZY1.This study provides insight into the role of auxin in determining rice leaf angle,potentially holding profound effects for the optimization of crop architecture.展开更多
[Objectives]This study was conducted to clarify the species composition of pathogens causing leaf spot disease in yellow peach in Loudi area of Hunan Province and to screen effective control agents.[Methods]Twenty lea...[Objectives]This study was conducted to clarify the species composition of pathogens causing leaf spot disease in yellow peach in Loudi area of Hunan Province and to screen effective control agents.[Methods]Twenty leaf spot samples were collected from yellow peach planting bases in Lengshuijiang and Shuangjiang,Loudi City.Two isolates were obtained through tissue isolation and purification.Identification was performed based on morphological characteristics combined with rDNA-ITS and Bt-2a gene sequence analysis and pathogenicity assay.[Results]The pathogen causing yellow peach leaf spot in this area was Botryosphaeria dothidea.[Conclusions]This study provides a reference basis for the prevention and control of yellow peach leaf spot in Loudi area.展开更多
The high hydrophilicity of pineapple leaf fibres(PALF)limits their use in cement-and gypsum-based composites exposed to moisture.This study evaluates,for the first time,the combined effect of palm kernel oil and beesw...The high hydrophilicity of pineapple leaf fibres(PALF)limits their use in cement-and gypsum-based composites exposed to moisture.This study evaluates,for the first time,the combined effect of palm kernel oil and beeswax on the hygroscopic resistance and mechanical stability of PALF.The fibres were functionalised with three formulations(oil,wax,and a 1:2 oil/wax blend)applied at different mass ratios(CR=0.5-2).Treatments increased the average bundle diameter by up to+46%(238μm)and reduced density down to 1.06 g/cm^(3).Hygroscopically,water absorption decreased from 202.4%(raw fibres)to 76.3%(CR=2),representing a maximum reduction of 59.4%in saline medium,while moisture regain dropped from 27.9%to 14.6%(−47.7%).The oil/wax blend proved most effective,simultaneously reducing water absorption(−51.2%)and moisture regain(−46.8%)at CR=1.Mechanically,the fibres retained tensile strength(415.2 vs.460.8 MPa,+11%at CR=1)and exhibited enhanced ductility(+62.5%,with elongation at break increasing from 1.6%to 2.6%),without significantly altering Young’s modulus(12.3 to 10 GPa).Water absorption kinetics were accurately described by the Czel and Mohsenin models(R^(2)>0.98).These findings clearly demonstrate that bio-based lipid coatings can provide an eco-friendly alternative to conventional chemical treatments.They improve hygroscopic resistance and preserve mechanical integrity of PALF,providing original quantitative data for their integration into durable cement-and gypsum-based composites subjected to humid or cyclic wet-dry conditions.展开更多
Hydraulic theory predicts a positive coupling between leaf hydraulic conductance(K_(leaf))and stomatal conductance(g_(s));however,this theory has not been fully supported by observations,and underlying mechanisms are ...Hydraulic theory predicts a positive coupling between leaf hydraulic conductance(K_(leaf))and stomatal conductance(g_(s));however,this theory has not been fully supported by observations,and underlying mechanisms are poorly understood.Partitioning K_(leaf)into inside-xylem(K_(x))and outside-xylem(K_(ox))components offers a refined framework for elucidating the regulation of g_(s) by leaf hydraulics.While optimal planting density may enhance water use efficiency(WUE)through modulation of g_(s),corresponding changes in leaf hydraulic properties and their influence on gas exchange remain unclear.We examined relationships among K_(x),K_(ox),g_(s),leaf photosynthetic rate(A_(N)),and WUE,and analyzed the structural determinants of K_(ox)in cotton grown under eight planting densities:12,18,24,36,48,60,72,and 84 plants m^(–2).Results showed that as planting density increased,K_(leaf)and A_(N) remained stable,whereas K_(ox)and g_(s) declined significantly.Leaf thickness and the volume fraction of inter-cellular air space were key structural factors influencing K_(ox).Neither K_(leaf)nor K_(x)correlated with A_(N) or g_(s);however,K_(ox)exhibited a significant positive correlation with g_(s).Furthermore,K_(ox)was negatively correlated with WUE.These findings indicate that K_(ox)modulates g_(s) to minimize water loss without compromising A_(N),thereby enhancing WUE in cotton across varying planting densities.展开更多
The establishment of plantations has become a critical approach for reducing greenhouse gas emissions,particularly in fragile environments with carbon sequestration potential.In karst areas,plantations based on fastgr...The establishment of plantations has become a critical approach for reducing greenhouse gas emissions,particularly in fragile environments with carbon sequestration potential.In karst areas,plantations based on fastgrowing afforestation species made significant contributions to enhancing carbon sequestration.However,the impact of understory vegetation on carbon accumulation remains unclear.Especially,the carbon accumulation associated with litter produced during the replacement of understory species receives insufficient attention,which leads to the neglect of the carbon sequestration potential in plantations of karst areas.Leaf is a crucial organ that links the litter production.To explore how leaf traits adapt to competitive environments and drive litter carbon accumulation during understory species replacement,this study observed leaf traits and litter carbon content changes in three types of plantations in the Liujiang River Basin,a typical karst area.A total of 37 sampling plots were selected for field investigation over a twoyear period.Leaf traits,species diversity,vegetation coverage,and litter carbon characteristics in understory vegetation were measured.Variance analysis,allometric equations,and path analysis were used for data analysis.The results showed that most understory species adopted a biomass conservation strategy under high-coverage conditions(>44.27%)and expanded competitive leaf area under low-coverage conditions(<44.27%).However,Bidens pilosa and Miscanthus floridulus exhibited strong competitiveness during understory species replacement.They showed an expansion of competitive leaf area under high-coverage conditions.This competitive strategy reduced species diversity and community specific leaf area.But the rapid expansion of Bidens pilosa and Miscanthus floridulus increased understory vegetation coverage,and their increased specific leaf area facilitated leaf shedding,resulting in significant litter weight accumulation(P<0.05),thereby enhancing litter carbon content per unit area.These competitive strategies were key driving factors for the increase in litter carbon content per square meter,which reached a maximum of 49.6% higher than that in natural grasslands.And the maximum increase in litter carbon accumulation derived from understory vegetation reached 3.37 times from 2023 to 2024 in plantations.In the understory vegetation of plantations,the competitive strategies reflected by leaf adaptation of key competitive species are critical factors influencing litter carbon accumulation.Future research could deeply explore the carbon sequestration effects resulting from the dynamic changes in competition within the understory vegetation of plantations.展开更多
Tropospheric ozone(O_(3))is a harmful air pollutant negatively impacting forest health,causing O_(3)-specific visible foliar injury(O_(3)VFI).Ozone monitoring in forests has usually implemented by passive samplers,alt...Tropospheric ozone(O_(3))is a harmful air pollutant negatively impacting forest health,causing O_(3)-specific visible foliar injury(O_(3)VFI).Ozone monitoring in forests has usually implemented by passive samplers,although they cannot detect the diurnal peak when a significant part of stomatal O_(3)uptake occurs.This results into uncertainties for the calculation of stomatal O_(3)uptake.This study compares the stomatal-flux-based POD1(phytotoxic ozone dose above a threshold of 1 nmol m^(-2)s^(-1))for forest trees/shrubs estimated from data collected by either passive samplers or active O_(3)monitors to evaluate O_(3)damage to plants in terms of O_(3)VFI in the Southern Alps.The study was conducted over two years(2018-2019)in a mountainous Alpine area(Valle Stura,Italy).An integrative monitoring station for active O_(3)monitoring,as well as passive O_(3)monitors,were installed in an open field area(OFD).The O_(3)VFI was investigated in woody species in the light exposed sampling Site(LESS—Betula pendula,Fagus sylvatica,Larix decidua,Populus tremula,Salix caprea,Rubus sp.and Vaccinium myrtillus)in late summer according to the international co-operative programme on assessment and monitoring of air pollution effects on forests(ICP Forests)manual.The results confirmed that Fagus sylvatica and Rubus sp.are O_(3)-sensitive species showing relatively high POD1(>20 mmol m-2),while Larix decidua is O_(3)-tolerant.We derived flux-based critical levels(CL)corresponding to the presence of O_(3)VFI(5,25,and 50%of symptomatic plants along the LESS)from flux-effect relationships for forest protection against O_(3)VFI.The results support the hypothesis that passive samplers cannot detect episodic high stomatal O₃fluxes(>1 nmol m^(-2)s^(-1)).According to the active monitoring,the CL for O_(3)VFI occurrence was estimated to be 17.1 mmol m-2 POD1 for 25%presence and 34.3 mmol m-2 POD1 for 50%presence of symptomatic plants,while passive samplers underestimated POD1 values for CL calculations by 17%on average,with underestimation increasing at higher CL thresholds.The findings demonstrate that active monitoring refines CLs towards a proper quantitative assessment of O_(3)impact,particularly in capturing peak flux events that are crucial for evaluating plant damage and emphasizes the importance of active O₃monitoring for reliable forest health assessments.展开更多
Four modern hybrid and four japonica rice varieties differing in biomass,yield,and daily biomass production rate during the grain-filling period(DBPGF),were used to reveal the eco-physiological photosynthetic characte...Four modern hybrid and four japonica rice varieties differing in biomass,yield,and daily biomass production rate during the grain-filling period(DBPGF),were used to reveal the eco-physiological photosynthetic characteristics of high-yield and high-efficiency rice.Varietal differences were analyzed in leaf and canopy photosynthetic parameters,associated leaf morphological and anatomical traits(e.g.,stomatal density,vein density,mesophyll cell arrangement),as well as differences in canopy light interception and leaf area index,and their effects on yield and biomass accumulation.Hybrid rice with yield higher than 11.0 t/hm^(2) and DBPGF higher than 200 kg/(hm^(2)·d),and japonica rice with yield higher than 9.0 t/hm^(2) and DBPGF higher than 200 kg/(hm^(2)·d),were classified as high-yield and high-efficiency varieties;other varieties were considered general types.Based on this criterion,two hybrid(Yongyou 2640 and Shanyou 63)and two japonica varieties(Huaidao 5 and Nangeng 5718)were categorized as high-yield and high-efficiency types,while the remaining two hybrid(Liangyoupeijiu and C Liangyou 513)and two japonica varieties(Suxiu 867 and Yangnongdao 1)were classified as general types.Results indicated that high-yield and high-efficiency varieties generally have higher leaf and canopy photosynthesis,superior leaf stomatal,vascular,and mesophyll structures that facilitate CO_(2)diffusion and hydraulic transport,higher canopy light transmittance,and slower leaf area attenuation.Rice yield and biomass were positively correlated with photosynthetic parameters and closely linked to associated photosynthetic traits.Efficient rice production was attributed to coordinated improvements in leaf structure,canopy architecture,and delayed leaf area attenuation.This study provides important theoretical guidance for breeding high-efficiency rice varieties.展开更多
Detailed individual tree crown segmentation is highly relevant for the detection and monitoring of Fraxinus excelsior L.trees affected by ash dieback,a major threat to common ash populations across Europe.In this stud...Detailed individual tree crown segmentation is highly relevant for the detection and monitoring of Fraxinus excelsior L.trees affected by ash dieback,a major threat to common ash populations across Europe.In this study,both fine and coarse crown segmentation methods were applied to close-range multispectral UAV imagery.The fine tree crown segmentation method utilized a novel unsupervised machine learning approach based on a blended NIR-NDVI image,whereas the coarse segmentation relied on the segment anything model(SAM).Both methods successfully delineated tree crown outlines,however,only the fine segmentation accurately captured internal canopy gaps.Despite these structural differences,mean NDVI values calculated per tree crown revealed no significant differences between the two approaches,indicating that coarse segmentation is sufficient for mean vegetation index assessments.Nevertheless,the fine segmentation revealed increased heterogeneity in NDVI values in more severely damaged trees,underscoring its value for detailed structural and health analyses.Furthermore,the fine segmentation workflow proved transferable to both individual UAV images and orthophotos from broader UAV surveys.For applications focused on structural integrity and spatial variation in canopy health,the fine segmentation approach is recommended.展开更多
Background As living standards improve,consumers are placing greater emphasis on the enhancement of fish flesh quality,making its improvement increasingly critical.Plant-derived polysaccharides positively affect the i...Background As living standards improve,consumers are placing greater emphasis on the enhancement of fish flesh quality,making its improvement increasingly critical.Plant-derived polysaccharides positively affect the improvement of animal flesh quality.Panax ginseng leaf polysaccharides(PGLP)have a similar composition and lower cost compared with Panax ginseng root polysaccharides.However,its function and application effects in grass carp(Ctenopharyngodon idella)are unclear.Methods A total of 540 sub-adult grass carp(679±1.29 g),one of the important economic fish species,were used as experimental models and fed diets supplemented with 0,100,200,300,400,or 500 mg/kg PGLP for 60 d.After 60 d,grass carp were weighed,and their muscles were collected to explore the effects of PGLP on the growth and development of myofibers and energy metabolism-related parameters.Results Our study found that PGLP increased the growth performance and muscle nutritional composition as well as improved muscle hardness,springiness,cohesiveness,chewiness,and hyperplasia of myofibers of sub-adult grass carp.Besides,PGLP promoted muscle energy metabolism by increasing creatine,glycogen,pyruvate,and acetyl-CoA contents and creatine kinase(CK),pyruvate kinase(PK),phosphofructokinase(PFK),and hexokinase(HK)activities,while decreasing lactate dehydrogenase(LDH)activity and lactate content in fish muscle.Finally,our study found that PGLP enhanced mitochondrial function by increasing the protein expression of mitochondrial complexes I–V,biogenesis,and fusion and decreasing autophagy and fission in fish muscle.Conclusions PGLP improved growth performance and flesh quality of sub-adult grass carp,which may be related to enhancing hyperplasia of myofibers by promoting energy metabolism.We concluded that the recommended amount of PGLP in sub-adult grass carp feed to optimize growth performance is 100–200 mg/kg.This study provides a theoretical basis for the application of PGLP in fish feed and for the analysis of the mechanism of nutrition and feed regulating fish flesh quality,which is of great significance.展开更多
Urban forests are highly multifunctional and provide numerous ecological functions.Plant functional traits individually or jointly influence the ecological multifunctionality of tree species(TS-EMF)and can also modify...Urban forests are highly multifunctional and provide numerous ecological functions.Plant functional traits individually or jointly influence the ecological multifunctionality of tree species(TS-EMF)and can also modify TSEMF in response to environmental changes.However,there has been limited exploration of multitrait combinations for predicting TS-EMF across seasons and of trait thresholds that enhance TS-EMF.Here,for 10 dominant tree species in urban forests of Northeast China,14 traits were measured and four aboveground and three belowground ecological functions assessed in three seasons.Ecological functions and TS-EMF differed significantly throughout the seasons(P<0.05).Synergistic relationships were found between carbon sequestration and oxygen release,between cooling and humidification,and between organic carbon accumulation and nutrient cycling.Notably,aboveground multifunctionality played a leading role in TS-EMF.With seasonal changes,resource allocation shifted toward traits related to resource acquisition rather than conservation to maintain TS-EMF.The combination of traits that predicted TS-EMF varied by type,accounting for up to 66.45%of the variation.TS-EMF was primarily driven by leaf structure in spring and by nutrient accumulation in autumn.Leaf carbon content(LCC)consistently served as a stabilizing factor for predicting TS-EMF across seasons.At 36.5-36.8 mg g^(-1),LCC had its optimal effect on TS-EMF.Other traits in combination that positively influence total TS-EMF include leaf nitrogen content(3.43-3.45 mg g^(-1)),leaf phosphorus content(0.80-0.83 mg g^(-1)),and leaf area(65.86-68.43 cm^(2)).Within these specified trait thresholds,Morus alba and Quercus mongolica were identified as key species.These findings suggest that the trade-off between various ecological functions can be managed by altering plant traits across seasons.This approach could provide a theoretical foundation for enhancing the TS-EMF of urban forests through trait-based management,offering practical guidance for selecting tree species.展开更多
Rat nerve growth factor and total flavonoids from hawthorn leaf contribute to the recovery of neurological function after spinal cord injury,including traumatic,non-traumatic spinal cord injuries.However,it remains ch...Rat nerve growth factor and total flavonoids from hawthorn leaf contribute to the recovery of neurological function after spinal cord injury,including traumatic,non-traumatic spinal cord injuries.However,it remains challenging to efficiently deliver nerve growth factor and total flavonoids from hawthorn leaf to spinal cord injury sites,ensure their sustained release,and minimize further damage.In the present study,we chose a biocompatible and biodegradable gelatin as the substrate,which was crosslinked with the natural biological crosslinker genipin to form a gelatin-genipin hydrogel carrier for the slow release of nerve growth factor and total flavonoids from hawthorn leaf in spinal cord injury sites.The prepared gelatin-genipin hydrogel had good injectable properties and photothermal effects.Furthermore,when the hydrogel with 2%genipin,200 ng/mL nerve growth factor,and 320μg/mL total flavonoids from hawthorn leaf was combined with near infrared irradiation,there was a slow release of total flavonoids from hawthorn leaf and nerve growth factor,reduced oxidative stress,an improved inflammatory microenvironment,and accelerated angiogenesis and axonal regeneration via inhibition of the nuclear factor kappa-B signaling pathway,thereby promoting recovery from spinal cord injury.Collectively,our results indicate that this new hydrogel may improve the prognosis of spinal cord injury,and may represent a new strategy for treating spinal cord injury.展开更多
Leaf morphology constitutes a key component of the ideotype,and optimal leaf rolling contributes to compact plant architecture.Rapeseed(Brassica napus)is an important oilseed crop;however,the genetic mechanisms underl...Leaf morphology constitutes a key component of the ideotype,and optimal leaf rolling contributes to compact plant architecture.Rapeseed(Brassica napus)is an important oilseed crop;however,the genetic mechanisms underlying leaf shape development remain poorly understood,and corresponding germplasm resources for genetic improvement are limited.In this study,we identified a dominant mutant,INSIDE-ROLLING LEAF1(IRL1),which exhibits inward leaf rolling due to defective mesophyll cell development.The mutant also displays drooping siliques and a semi-dwarf phenotype,accompanied by a reduction of one to two effective branches.Through map-based cloning and functional complementation assays,we confirmed BnaC02G0201100ZS as the causal gene IRL1.This gene encodes LATERAL ORGAN BOUNDARIES DOMAIN6(BnaC02.LBD6).The phenotypic alterations in the IRL1 mutant result from elevated expression of BnaC02.LBD6,driven by a single nucleotide substitution within a DNA binding site in its promoter region.Overexpression of Bna C02.LBD6 recapitulated the IRL1 mutant phenotype,confirming its functional role.Haplotype analysis revealed a rare allelic variant in the BnaC02.LBD6 promoter associated with the unique leaf morphology of IRL1.Transcriptomic profiling indicated significant differential expression of genes involved in adaxial–abaxial leaf polarity establishment,secondary metabolic pathways,and hormone signaling networks.Our findings provide novel insights into the genetic regulation of leaf morphogenesis in rapeseed and offer valuable genetic resources for optimizing plant architecture in breeding programs.展开更多
To ascertain the genetic diversity of gray leaf spot pathogen on Dictamnus dasycarpus popoulation in Heilongjiang Province,a total of 57 strains of Paracercospora dictamnicola were isolated and purified from the disea...To ascertain the genetic diversity of gray leaf spot pathogen on Dictamnus dasycarpus popoulation in Heilongjiang Province,a total of 57 strains of Paracercospora dictamnicola were isolated and purified from the diseased samples collected from five Chinese herbal medicine planting areas in Heilongjiang Province between the years of 2021 and 2022.Repetitive extragenic palindromic polymerase chain reaction(Rep–PCR)was used to amplify 57 isolates of gray leaf spot pathogen on D.dasycarpus from different regions of Heilongjiang Province.The polymorphic bands amplified by three sets of primers accounted for more than 80%.Cluster analysis results showed that at a similarity coefficient of 0.67,the gray leaf spot pathogen on D.dasycarpus in Heilongjiang Province could be divided into five major genetic groups.Genetic diversity parameter analysis indicated that there were certain differences in genetic richness among the geographic populations of gray leaf spot pathogen on D.dasycarpus from different regions.Analysis of molecular variance(AMOVA)revealed that genetic variation among strains mainly originated within populations.The genetic differentiation and relationships of gray leaf spot pathogen on D.dasycarpus from different geographic regions of Heilongjiang Province indicated that genetic differentiation and kinship among populations were somewhat related to their geographic distance.The greater the geographic distance,the higher the genetic differentiation coefficient,and the lower the genetic uniformity among populations.展开更多
The leaf nitrogen(N)to phosphorus(P)ratio(N:P)is a critical indicator of nutrient dynamics and ecosystem function.Investigating temporal variations in leaf N:P can provide valuable insights into how plants adapt to en...The leaf nitrogen(N)to phosphorus(P)ratio(N:P)is a critical indicator of nutrient dynamics and ecosystem function.Investigating temporal variations in leaf N:P can provide valuable insights into how plants adapt to environmental changes and nutrient availability.However,limited research has been conducted on long-term temporal leaf N:P variation over a range of temperature zones.Using long-term monitoring data from the Chinese Ecosystem Research Network(CERN),we investigated temporal changes in leaf N and P stoichiometry for 50 dominant tree species from 10 typical forest sites across temperate and subtropical regions,and identified the underlying mechanisms driving these changes.For both regions combined,leaf P concentration of the 50 dominant tree species decreased(20.6%),whereas leaf N:P increased(52.0%)from 2005 to 2020.Leaf P decreased and leaf N:P increased in 67% and 69% of the tree species,respectively.The leaf N:P increase was primarily driven by the tree species in eastern subtropical forests,where global change factors and soil nutrients explained 68% of leaf N:P variation.The P limitation exhibited by tree species in eastern subtropical forest ecosystems intensified over time,and elevated temperature and CO_(2) levels,coupled with decreased soil available P concentrations,appear to be the main factors driving long-term leaf N:P increases in these forests.Investigating long-term variations in soil nutrients together with global change factors will improve our understanding of the nutrient status of forest ecosystems in the context of global change and will support effective forest ecosystem management.展开更多
Photosynthesis is one the most important chemical reaction in plants,and it is the ultimate energy source of any living organisms.The light and dark reactions are two essential phases of photosynthesis.Light reaction ...Photosynthesis is one the most important chemical reaction in plants,and it is the ultimate energy source of any living organisms.The light and dark reactions are two essential phases of photosynthesis.Light reaction harvests light energy to synthesize ATP and NADPH through an electron transport chain,and as well as giving out O_(2);dark reaction fixes CO_(2) into six carbon sugars by utilizing NADPH and energy from ATP.Subsequently,plants convert optical energy into chemical energy for maintaining growth and development through absorbing light energy.Here,firstly,we highlighted the biological importance of photosynthesis,and hormones and metabolites,photosynthetic and regulating enzymes,and signaling components that collectively regulate photosynthesis in tomato.Next,we reviewed the advances in tomato photosynthesis,including two aspects of genetic basis and genetic improvement.Numerous genes regulating tomato photosynthesis are gradually uncovered,and the interaction network among those genes remains to be constructed.Finally,the photosynthesis occurring in fruit of tomato and the relationship between photosynthesis in leaf and fruit were discussed.Leaves and fruits are photosynthate sources and sinks of tomato respectively,and interaction between photosynthesis in leaf and fruit exists.Additionally,future perspectives that needs to be addressed on tomato photosynthesis were proposed.展开更多
基金supported by the National Natural Science Foundation of China(32460329)the Bintuan Science&Technology Program(2024AB075)to L.H+2 种基金the National Natural Science Foundation of China(32360279)an open program from the Key Laboratory of Protection and Utilization of Biological Resources in the Tarim Basin(BRZD2004)a provincial talentintroduction program of Xinjiang Uygur Autonomous Region to D.H.
文摘The trade-off between leaf size and leafing intensity(i.e.,the number of leaves per unit stem size)is a key axis of trait covariation across the diversity of plant foliage deployment.However,the functional significance of leafing intensity and its possible combinations with leaf size in dealing with water limitation remains unclear.Using Populus euphratica as an illustrative tree species growing in hyper-arid climates,we investigated how leaf size and leafing intensity co-varied under varying water stresses.In the Ebinor lowlands and the upper reaches of the Tarim River(NW China),we sampled>1800 current-year twigs from 505 trees across 14 sites along a climatic gradient characterized by precipitation,potential evapotranspiration and vapor pressure deficit.Leafing intensity based on stem mass(LIM)decreased with climatic aridity,primarily due to greater stem mass,but not fewer leaves.This indicates a higher investment in structural support for leaf attachment under water stress.Both leaf area and mass decreased with LIM at a lower-than-proportional rate,with the decrease in leaf size being more pronounced under drier climates.This suggests that higher LIM incurs a high cost of reducing leaf size in water-limited habitats.These findings challenge the assumption that higher leafing intensity always confers an advantage ready for environmental stresses due to higher developmental flexibility offered by more axillary buds.Rather,we propose that a strategy of lower leafing intensity,with greater structural support for leaf attachment and less compromise in leaf size,can be advantageous under water limitation.
文摘The leafing rates of fourteen rice varieties were measured in a sowing-time experiment, and a rapid leafing genotype at the vegetative growth stage was discovered in an indica variety Yanhui 559. The leaf number on the main culm of Yanhui 559 was always 4-5 leaves more than that of Lemont, and the leafing rate of Yanhui 559 was significantly higher than that of Lemont based on similar growth durations from sowing to heading. Furthermore, the difference of the leafing rate was significant at the vegetative growth stage, but not distinctive at the panicle initiation stage. Genetic analysis of the leafing rates in the two backcross populations of Yanhui 559 and Lemont showed that major and quantitative genes controlled the expression of rapid leafing character. Based on results of investigation for some plants with similar growth durations in the backcrossing populations, the rapid leafing genotypes exhibited earlier tillering and more tiller numbers per plant, and its yield components including the number of panicles per plant and number of grains per panicle were superior to those of the slow leafing genotypes. Further research and application feasibility of the rapid leafing genotype in breeding were discussed.
基金supported by Central Finance for the Forestry Science and Technology Promotion Demonstration Project([2024]TG13)the National Science Foundation of China(Grant No.32201643)the Key research projects of Yibin,Research and Integrated Demonstration and Key Technologies for Smart Bamboo Industry(Grant No.YBZD2024-1).
文摘Anthocyanin-rich foliage plants hold important applications in the pharmaceutical industry and the tea sector,beyond their significant ornamental value.These plants also possess biological and ecological importance,contributing to reproduction,defense against natural enemies,and adaptation to environmental changes.Thus,a deeper understanding of their leaf coloration will be essential for both practical applications and theoretical understanding.The present study comprehensively reviews the factors influencing anthocyanin metabolism,including biosynthesis,transport,degradation,transcription factors(TF_(S)),post-transcriptional regulation,post translation regulation.Next,we summarize the application of omics technologies in unveiling the mechanisms of anthocyanin synthesis in leaves.Furthermore,we review the molecular mechanisms by which environmental factors regulate leaf coloration by inducing anthocyanin biosynthesis.Lastly,the study addresses unresolved issues in the research of plant leaf coloration and proposes future research directions in this field.This study is anticipated to provide a valuable reference for the study of plant leaf coloration.
基金supported by the National Natural Science Foundation of China(32301845)GuangDong Basic and Applied Basic Research Foundation(2022A1515012339)+3 种基金the National Key R&D Program of China(2024YFD1200801)Seed industry revitalization project of special fund for rural revitalization strategy in Guangdong Province(2024-NPY-00-001)Modern Seed Industry Innovation Capacity Enhancement Progject of Guangdong Academy of Agricultural Sciences,Elite Rice Plan of GDRRI(2023YG01)Guangdong Key Laboratory of Rice Science and Technology(2023B1212060042).
文摘Leaf thickness in rice critically influences photosynthetic efficiency and yield,yet its genetic basis remains poorly understood,with few functional genes previously characterized.In this study,we employ a pangenome-wide association study(Pan-GWAS)on 302 diverse rice accessions from southern China,identifying 49 quantitative trait loci(QTLs)associated with leaf thickness.The most significant locus,qLT9,is fine-mapped to a 79-kb region on chromosome 9.Transcriptomic and genomic sequence analyses identify LOC_Os09g33480,which encodes a protein belonging to Multiple Organellar RNA Editing Factor family,as the key candidate gene.Overexpression and complementation transgenic experiments confirm LOC_Os09g33480(OsLT9)as the functional gene underlying qLT9,demonstrating a 24-bp Indel in its promoter correlates with the expression levels and leaf thickness.Notably,OsLT9 overexpression lines show not only thicker leaf,but also significantly enhanced photosynthetic efficiency and grain yield,establishing a link between leaf thickness modulation and yield enhancement.Population genomic analyses indicate strong selection for OsLT9 during domestication and breeding,with modern cultivars favoring thick leaf haplotype of OsLT9.This study establishes OsLT9 as a key regulator controlling leaf thickness in rice,and provides a valuable genetic resource for molecular breeding of high-yielding rice through optimization of plant architecture.
基金Funding was provided by the Science&Technology Fundamental Resources Investigation Program(2022FY202100)Shaanxi Institute of Zoology Program(Y2412093J18)Shaanxi Academy of Sciences Program(2024P-08,2025k-24).
文摘This paper describes two new species in the genus Cneorella Medvedev&Dang,1981 from China:C.flava sp.nov.and C.hainanensis sp.nov.External morphological photographs and illustrations of the male genitalia of these new species are provided.A key to the Chinese known species in this genus is given.
基金funded by the National Natural Science Foundation of China(31871592)the Fundamental Research Funds for the Central Universities(2042022kf0015)+1 种基金the Creative Research Groups of the Natural Science Foundation of Hubei Province(2020CFA009)the Project for Technology Innovation of Hubei Province(2024BBA005).
文摘Leaf angle is a pivotal agronomic trait that significantly influences crop architecture and yield.Plant hormones,such as auxin,play a critical role in regulating leaf angle;however,the underlying molecular mechanisms remain to be fully elucidated.Here,we reveal that the auxin response factor gene,OsARF12,which is highly expressed in the leaf lamina joint,negatively regulates rice(Oryza sativa L.)leaf angle via affecting shoot gravitropism.Overexpression lines of OsARF12 exhibit more erect leaf angles,while the osarf12 mutants display enlarged leaf angles compared to the wild type.Further studies demonstrate that OsARF12 directly activates the expression of Loose Plant Architecture1(LPA1)and LAZY1 by binding to their promoters.The osarf12 mutant presents impaired shoot gravitropism,a phenotype consistent with that of the lpa1 and lazy1 mutants.Collectively,we elucidate the biological functions of OsARF12,which modulates leaf angle through its impact on shoot gravitropism by regulating the expression levels of LPA1 and LAZY1.This study provides insight into the role of auxin in determining rice leaf angle,potentially holding profound effects for the optimization of crop architecture.
基金Supported by Natural Science Foundation of Hunan Province(2024JJ72572025JJ70341)+1 种基金Research and Innovation Project of Hunan University of Humanities,Science and Technology(ZSCX2024Y11)Undergraduate Innovation and Entrepreneurship Training Program(S202410553043).
文摘[Objectives]This study was conducted to clarify the species composition of pathogens causing leaf spot disease in yellow peach in Loudi area of Hunan Province and to screen effective control agents.[Methods]Twenty leaf spot samples were collected from yellow peach planting bases in Lengshuijiang and Shuangjiang,Loudi City.Two isolates were obtained through tissue isolation and purification.Identification was performed based on morphological characteristics combined with rDNA-ITS and Bt-2a gene sequence analysis and pathogenicity assay.[Results]The pathogen causing yellow peach leaf spot in this area was Botryosphaeria dothidea.[Conclusions]This study provides a reference basis for the prevention and control of yellow peach leaf spot in Loudi area.
文摘The high hydrophilicity of pineapple leaf fibres(PALF)limits their use in cement-and gypsum-based composites exposed to moisture.This study evaluates,for the first time,the combined effect of palm kernel oil and beeswax on the hygroscopic resistance and mechanical stability of PALF.The fibres were functionalised with three formulations(oil,wax,and a 1:2 oil/wax blend)applied at different mass ratios(CR=0.5-2).Treatments increased the average bundle diameter by up to+46%(238μm)and reduced density down to 1.06 g/cm^(3).Hygroscopically,water absorption decreased from 202.4%(raw fibres)to 76.3%(CR=2),representing a maximum reduction of 59.4%in saline medium,while moisture regain dropped from 27.9%to 14.6%(−47.7%).The oil/wax blend proved most effective,simultaneously reducing water absorption(−51.2%)and moisture regain(−46.8%)at CR=1.Mechanically,the fibres retained tensile strength(415.2 vs.460.8 MPa,+11%at CR=1)and exhibited enhanced ductility(+62.5%,with elongation at break increasing from 1.6%to 2.6%),without significantly altering Young’s modulus(12.3 to 10 GPa).Water absorption kinetics were accurately described by the Czel and Mohsenin models(R^(2)>0.98).These findings clearly demonstrate that bio-based lipid coatings can provide an eco-friendly alternative to conventional chemical treatments.They improve hygroscopic resistance and preserve mechanical integrity of PALF,providing original quantitative data for their integration into durable cement-and gypsum-based composites subjected to humid or cyclic wet-dry conditions.
基金financially supported by the Tianshan Talent Development Program,China for Yali Zhangthe Natural Science Foundation of Xinjiang Production and Construction Corps,China(2024DA002)the Earmarked Fund for XJARS-Cotton,China(XJARS-03)。
文摘Hydraulic theory predicts a positive coupling between leaf hydraulic conductance(K_(leaf))and stomatal conductance(g_(s));however,this theory has not been fully supported by observations,and underlying mechanisms are poorly understood.Partitioning K_(leaf)into inside-xylem(K_(x))and outside-xylem(K_(ox))components offers a refined framework for elucidating the regulation of g_(s) by leaf hydraulics.While optimal planting density may enhance water use efficiency(WUE)through modulation of g_(s),corresponding changes in leaf hydraulic properties and their influence on gas exchange remain unclear.We examined relationships among K_(x),K_(ox),g_(s),leaf photosynthetic rate(A_(N)),and WUE,and analyzed the structural determinants of K_(ox)in cotton grown under eight planting densities:12,18,24,36,48,60,72,and 84 plants m^(–2).Results showed that as planting density increased,K_(leaf)and A_(N) remained stable,whereas K_(ox)and g_(s) declined significantly.Leaf thickness and the volume fraction of inter-cellular air space were key structural factors influencing K_(ox).Neither K_(leaf)nor K_(x)correlated with A_(N) or g_(s);however,K_(ox)exhibited a significant positive correlation with g_(s).Furthermore,K_(ox)was negatively correlated with WUE.These findings indicate that K_(ox)modulates g_(s) to minimize water loss without compromising A_(N),thereby enhancing WUE in cotton across varying planting densities.
基金supported by the Scientific Research Foundation supported by Yunnan Agricultural University(A3012024035044)International Cooperation and Exchange of the National Natural Science Foundation of China(No.42361144885).
文摘The establishment of plantations has become a critical approach for reducing greenhouse gas emissions,particularly in fragile environments with carbon sequestration potential.In karst areas,plantations based on fastgrowing afforestation species made significant contributions to enhancing carbon sequestration.However,the impact of understory vegetation on carbon accumulation remains unclear.Especially,the carbon accumulation associated with litter produced during the replacement of understory species receives insufficient attention,which leads to the neglect of the carbon sequestration potential in plantations of karst areas.Leaf is a crucial organ that links the litter production.To explore how leaf traits adapt to competitive environments and drive litter carbon accumulation during understory species replacement,this study observed leaf traits and litter carbon content changes in three types of plantations in the Liujiang River Basin,a typical karst area.A total of 37 sampling plots were selected for field investigation over a twoyear period.Leaf traits,species diversity,vegetation coverage,and litter carbon characteristics in understory vegetation were measured.Variance analysis,allometric equations,and path analysis were used for data analysis.The results showed that most understory species adopted a biomass conservation strategy under high-coverage conditions(>44.27%)and expanded competitive leaf area under low-coverage conditions(<44.27%).However,Bidens pilosa and Miscanthus floridulus exhibited strong competitiveness during understory species replacement.They showed an expansion of competitive leaf area under high-coverage conditions.This competitive strategy reduced species diversity and community specific leaf area.But the rapid expansion of Bidens pilosa and Miscanthus floridulus increased understory vegetation coverage,and their increased specific leaf area facilitated leaf shedding,resulting in significant litter weight accumulation(P<0.05),thereby enhancing litter carbon content per unit area.These competitive strategies were key driving factors for the increase in litter carbon content per square meter,which reached a maximum of 49.6% higher than that in natural grasslands.And the maximum increase in litter carbon accumulation derived from understory vegetation reached 3.37 times from 2023 to 2024 in plantations.In the understory vegetation of plantations,the competitive strategies reflected by leaf adaptation of key competitive species are critical factors influencing litter carbon accumulation.Future research could deeply explore the carbon sequestration effects resulting from the dynamic changes in competition within the understory vegetation of plantations.
基金supported by the INTERREG ALCOTRA project MITIMPACT,PNRR for Mission 4(Component 2,Notice 3264/2021,IR0000032)-ITINERIS-Italian Integrated Environmental Research Infrastructure System CUP B53C22002150006Project funded under the National Recovery and Resilience Plan(NRRP),Mission 4 Component 2 Investment 1.4-Call for tender No.3138 of December 16,2021,rectified by Decree n.3175 of December 18,2021 of Italian Ministry of University and Research funded by the European Union-NextGenerationEU,Award Number:Project code CN_00000033,Concession Decree No.1034 of June 17,2022 adopted by the Italian Ministry of University and Research,CUP,H43C22000530001 Project title“National Biodiversity Future Center-NBFC”(Spoke 3 and 5)AP and IP were supported by the FOR-CLIMSOC Programme,Project ID PN23090101,financed by the Ministry of Research,Innovation,and Digitization in Romania.
文摘Tropospheric ozone(O_(3))is a harmful air pollutant negatively impacting forest health,causing O_(3)-specific visible foliar injury(O_(3)VFI).Ozone monitoring in forests has usually implemented by passive samplers,although they cannot detect the diurnal peak when a significant part of stomatal O_(3)uptake occurs.This results into uncertainties for the calculation of stomatal O_(3)uptake.This study compares the stomatal-flux-based POD1(phytotoxic ozone dose above a threshold of 1 nmol m^(-2)s^(-1))for forest trees/shrubs estimated from data collected by either passive samplers or active O_(3)monitors to evaluate O_(3)damage to plants in terms of O_(3)VFI in the Southern Alps.The study was conducted over two years(2018-2019)in a mountainous Alpine area(Valle Stura,Italy).An integrative monitoring station for active O_(3)monitoring,as well as passive O_(3)monitors,were installed in an open field area(OFD).The O_(3)VFI was investigated in woody species in the light exposed sampling Site(LESS—Betula pendula,Fagus sylvatica,Larix decidua,Populus tremula,Salix caprea,Rubus sp.and Vaccinium myrtillus)in late summer according to the international co-operative programme on assessment and monitoring of air pollution effects on forests(ICP Forests)manual.The results confirmed that Fagus sylvatica and Rubus sp.are O_(3)-sensitive species showing relatively high POD1(>20 mmol m-2),while Larix decidua is O_(3)-tolerant.We derived flux-based critical levels(CL)corresponding to the presence of O_(3)VFI(5,25,and 50%of symptomatic plants along the LESS)from flux-effect relationships for forest protection against O_(3)VFI.The results support the hypothesis that passive samplers cannot detect episodic high stomatal O₃fluxes(>1 nmol m^(-2)s^(-1)).According to the active monitoring,the CL for O_(3)VFI occurrence was estimated to be 17.1 mmol m-2 POD1 for 25%presence and 34.3 mmol m-2 POD1 for 50%presence of symptomatic plants,while passive samplers underestimated POD1 values for CL calculations by 17%on average,with underestimation increasing at higher CL thresholds.The findings demonstrate that active monitoring refines CLs towards a proper quantitative assessment of O_(3)impact,particularly in capturing peak flux events that are crucial for evaluating plant damage and emphasizes the importance of active O₃monitoring for reliable forest health assessments.
基金supported by the National Natural Science Foundation of China(Grant Nos.32201890 and 32272197)the Special Funds for Scientific and Technological Innovation of Jiangsu province,China(Grant Nos.BE2022425 and BK20220017)+1 种基金the National Key R&D Program of China(Grant No.2022YFD2301401)the Priority Academic Program Development of Jiangsu Higher Education Institutions,China.
文摘Four modern hybrid and four japonica rice varieties differing in biomass,yield,and daily biomass production rate during the grain-filling period(DBPGF),were used to reveal the eco-physiological photosynthetic characteristics of high-yield and high-efficiency rice.Varietal differences were analyzed in leaf and canopy photosynthetic parameters,associated leaf morphological and anatomical traits(e.g.,stomatal density,vein density,mesophyll cell arrangement),as well as differences in canopy light interception and leaf area index,and their effects on yield and biomass accumulation.Hybrid rice with yield higher than 11.0 t/hm^(2) and DBPGF higher than 200 kg/(hm^(2)·d),and japonica rice with yield higher than 9.0 t/hm^(2) and DBPGF higher than 200 kg/(hm^(2)·d),were classified as high-yield and high-efficiency varieties;other varieties were considered general types.Based on this criterion,two hybrid(Yongyou 2640 and Shanyou 63)and two japonica varieties(Huaidao 5 and Nangeng 5718)were categorized as high-yield and high-efficiency types,while the remaining two hybrid(Liangyoupeijiu and C Liangyou 513)and two japonica varieties(Suxiu 867 and Yangnongdao 1)were classified as general types.Results indicated that high-yield and high-efficiency varieties generally have higher leaf and canopy photosynthesis,superior leaf stomatal,vascular,and mesophyll structures that facilitate CO_(2)diffusion and hydraulic transport,higher canopy light transmittance,and slower leaf area attenuation.Rice yield and biomass were positively correlated with photosynthetic parameters and closely linked to associated photosynthetic traits.Efficient rice production was attributed to coordinated improvements in leaf structure,canopy architecture,and delayed leaf area attenuation.This study provides important theoretical guidance for breeding high-efficiency rice varieties.
基金This study was conducted within the project FraxVir“Detection,characterisation and analyses of the occurrence of viruses and ash dieback in special stands of Fraxinus excelsior-a supplementary study to the FraxForFuture demonstration project”and receives funding via the Waldklimafonds(WKF)funded by the German Federal Ministry of Food and Agriculture(BMEL)and Federal Ministry for the Environment,Nature Conservation,Nuclear Safety and Consumer Protection(BMUV)administrated by the Agency for Renewable Resources(FNR)under grant agreement 2220WK40A4.
文摘Detailed individual tree crown segmentation is highly relevant for the detection and monitoring of Fraxinus excelsior L.trees affected by ash dieback,a major threat to common ash populations across Europe.In this study,both fine and coarse crown segmentation methods were applied to close-range multispectral UAV imagery.The fine tree crown segmentation method utilized a novel unsupervised machine learning approach based on a blended NIR-NDVI image,whereas the coarse segmentation relied on the segment anything model(SAM).Both methods successfully delineated tree crown outlines,however,only the fine segmentation accurately captured internal canopy gaps.Despite these structural differences,mean NDVI values calculated per tree crown revealed no significant differences between the two approaches,indicating that coarse segmentation is sufficient for mean vegetation index assessments.Nevertheless,the fine segmentation revealed increased heterogeneity in NDVI values in more severely damaged trees,underscoring its value for detailed structural and health analyses.Furthermore,the fine segmentation workflow proved transferable to both individual UAV images and orthophotos from broader UAV surveys.For applications focused on structural integrity and spatial variation in canopy health,the fine segmentation approach is recommended.
基金supported by National Natural Science Foundation of China(U23A20250)the earmarked fund for CARS(CARS-45)+2 种基金the National Key R&D Program of China(2023YFD2400600)Sichuan Provincial Science and Technology Innovation Talent Project(2023JDRC0043)Sichuan Innovation Team of National Modern Agricultural Industry Technology System(SCCXTD-2024-15)。
文摘Background As living standards improve,consumers are placing greater emphasis on the enhancement of fish flesh quality,making its improvement increasingly critical.Plant-derived polysaccharides positively affect the improvement of animal flesh quality.Panax ginseng leaf polysaccharides(PGLP)have a similar composition and lower cost compared with Panax ginseng root polysaccharides.However,its function and application effects in grass carp(Ctenopharyngodon idella)are unclear.Methods A total of 540 sub-adult grass carp(679±1.29 g),one of the important economic fish species,were used as experimental models and fed diets supplemented with 0,100,200,300,400,or 500 mg/kg PGLP for 60 d.After 60 d,grass carp were weighed,and their muscles were collected to explore the effects of PGLP on the growth and development of myofibers and energy metabolism-related parameters.Results Our study found that PGLP increased the growth performance and muscle nutritional composition as well as improved muscle hardness,springiness,cohesiveness,chewiness,and hyperplasia of myofibers of sub-adult grass carp.Besides,PGLP promoted muscle energy metabolism by increasing creatine,glycogen,pyruvate,and acetyl-CoA contents and creatine kinase(CK),pyruvate kinase(PK),phosphofructokinase(PFK),and hexokinase(HK)activities,while decreasing lactate dehydrogenase(LDH)activity and lactate content in fish muscle.Finally,our study found that PGLP enhanced mitochondrial function by increasing the protein expression of mitochondrial complexes I–V,biogenesis,and fusion and decreasing autophagy and fission in fish muscle.Conclusions PGLP improved growth performance and flesh quality of sub-adult grass carp,which may be related to enhancing hyperplasia of myofibers by promoting energy metabolism.We concluded that the recommended amount of PGLP in sub-adult grass carp feed to optimize growth performance is 100–200 mg/kg.This study provides a theoretical basis for the application of PGLP in fish feed and for the analysis of the mechanism of nutrition and feed regulating fish flesh quality,which is of great significance.
基金supported by the National Natural Science Foundation(32130068,32271634,and 32071597)CAS Key Laboratory of Forest Ecology and Silviculture,Institute of Applied Ecology,Chinese Academy of Sciences(KLFES-2025)。
文摘Urban forests are highly multifunctional and provide numerous ecological functions.Plant functional traits individually or jointly influence the ecological multifunctionality of tree species(TS-EMF)and can also modify TSEMF in response to environmental changes.However,there has been limited exploration of multitrait combinations for predicting TS-EMF across seasons and of trait thresholds that enhance TS-EMF.Here,for 10 dominant tree species in urban forests of Northeast China,14 traits were measured and four aboveground and three belowground ecological functions assessed in three seasons.Ecological functions and TS-EMF differed significantly throughout the seasons(P<0.05).Synergistic relationships were found between carbon sequestration and oxygen release,between cooling and humidification,and between organic carbon accumulation and nutrient cycling.Notably,aboveground multifunctionality played a leading role in TS-EMF.With seasonal changes,resource allocation shifted toward traits related to resource acquisition rather than conservation to maintain TS-EMF.The combination of traits that predicted TS-EMF varied by type,accounting for up to 66.45%of the variation.TS-EMF was primarily driven by leaf structure in spring and by nutrient accumulation in autumn.Leaf carbon content(LCC)consistently served as a stabilizing factor for predicting TS-EMF across seasons.At 36.5-36.8 mg g^(-1),LCC had its optimal effect on TS-EMF.Other traits in combination that positively influence total TS-EMF include leaf nitrogen content(3.43-3.45 mg g^(-1)),leaf phosphorus content(0.80-0.83 mg g^(-1)),and leaf area(65.86-68.43 cm^(2)).Within these specified trait thresholds,Morus alba and Quercus mongolica were identified as key species.These findings suggest that the trade-off between various ecological functions can be managed by altering plant traits across seasons.This approach could provide a theoretical foundation for enhancing the TS-EMF of urban forests through trait-based management,offering practical guidance for selecting tree species.
基金Guangxi Science and Technology Base and Talent Special Project,No.GuiKeAD24010037(to SZ)Guangxi Health Commission Self-Funded Research Project,No.Z-A20241029(to YL).
文摘Rat nerve growth factor and total flavonoids from hawthorn leaf contribute to the recovery of neurological function after spinal cord injury,including traumatic,non-traumatic spinal cord injuries.However,it remains challenging to efficiently deliver nerve growth factor and total flavonoids from hawthorn leaf to spinal cord injury sites,ensure their sustained release,and minimize further damage.In the present study,we chose a biocompatible and biodegradable gelatin as the substrate,which was crosslinked with the natural biological crosslinker genipin to form a gelatin-genipin hydrogel carrier for the slow release of nerve growth factor and total flavonoids from hawthorn leaf in spinal cord injury sites.The prepared gelatin-genipin hydrogel had good injectable properties and photothermal effects.Furthermore,when the hydrogel with 2%genipin,200 ng/mL nerve growth factor,and 320μg/mL total flavonoids from hawthorn leaf was combined with near infrared irradiation,there was a slow release of total flavonoids from hawthorn leaf and nerve growth factor,reduced oxidative stress,an improved inflammatory microenvironment,and accelerated angiogenesis and axonal regeneration via inhibition of the nuclear factor kappa-B signaling pathway,thereby promoting recovery from spinal cord injury.Collectively,our results indicate that this new hydrogel may improve the prognosis of spinal cord injury,and may represent a new strategy for treating spinal cord injury.
基金funded by the National Key Research and Development Program of China(2021YFF1000100)the Fundamental Research Funds for the Central Universities,China(2662023PY004)。
文摘Leaf morphology constitutes a key component of the ideotype,and optimal leaf rolling contributes to compact plant architecture.Rapeseed(Brassica napus)is an important oilseed crop;however,the genetic mechanisms underlying leaf shape development remain poorly understood,and corresponding germplasm resources for genetic improvement are limited.In this study,we identified a dominant mutant,INSIDE-ROLLING LEAF1(IRL1),which exhibits inward leaf rolling due to defective mesophyll cell development.The mutant also displays drooping siliques and a semi-dwarf phenotype,accompanied by a reduction of one to two effective branches.Through map-based cloning and functional complementation assays,we confirmed BnaC02G0201100ZS as the causal gene IRL1.This gene encodes LATERAL ORGAN BOUNDARIES DOMAIN6(BnaC02.LBD6).The phenotypic alterations in the IRL1 mutant result from elevated expression of BnaC02.LBD6,driven by a single nucleotide substitution within a DNA binding site in its promoter region.Overexpression of Bna C02.LBD6 recapitulated the IRL1 mutant phenotype,confirming its functional role.Haplotype analysis revealed a rare allelic variant in the BnaC02.LBD6 promoter associated with the unique leaf morphology of IRL1.Transcriptomic profiling indicated significant differential expression of genes involved in adaxial–abaxial leaf polarity establishment,secondary metabolic pathways,and hormone signaling networks.Our findings provide novel insights into the genetic regulation of leaf morphogenesis in rapeseed and offer valuable genetic resources for optimizing plant architecture in breeding programs.
基金Supported by the Green Plant Protection Project of Heilongjiang Province(2130108)Key R&D Program Project of Heilongjiang Province(2023ZX02B0502)Heilongjiang Province Rice Modern Agriculture Industry Technology Collaborative Innovation System Project(2025)。
文摘To ascertain the genetic diversity of gray leaf spot pathogen on Dictamnus dasycarpus popoulation in Heilongjiang Province,a total of 57 strains of Paracercospora dictamnicola were isolated and purified from the diseased samples collected from five Chinese herbal medicine planting areas in Heilongjiang Province between the years of 2021 and 2022.Repetitive extragenic palindromic polymerase chain reaction(Rep–PCR)was used to amplify 57 isolates of gray leaf spot pathogen on D.dasycarpus from different regions of Heilongjiang Province.The polymorphic bands amplified by three sets of primers accounted for more than 80%.Cluster analysis results showed that at a similarity coefficient of 0.67,the gray leaf spot pathogen on D.dasycarpus in Heilongjiang Province could be divided into five major genetic groups.Genetic diversity parameter analysis indicated that there were certain differences in genetic richness among the geographic populations of gray leaf spot pathogen on D.dasycarpus from different regions.Analysis of molecular variance(AMOVA)revealed that genetic variation among strains mainly originated within populations.The genetic differentiation and relationships of gray leaf spot pathogen on D.dasycarpus from different geographic regions of Heilongjiang Province indicated that genetic differentiation and kinship among populations were somewhat related to their geographic distance.The greater the geographic distance,the higher the genetic differentiation coefficient,and the lower the genetic uniformity among populations.
基金supported by the National Natural Science Foundation of China(No.42030509)the Special Project on National Science and Technology Basic Resources Investigation of China(No.2021FY100705).
文摘The leaf nitrogen(N)to phosphorus(P)ratio(N:P)is a critical indicator of nutrient dynamics and ecosystem function.Investigating temporal variations in leaf N:P can provide valuable insights into how plants adapt to environmental changes and nutrient availability.However,limited research has been conducted on long-term temporal leaf N:P variation over a range of temperature zones.Using long-term monitoring data from the Chinese Ecosystem Research Network(CERN),we investigated temporal changes in leaf N and P stoichiometry for 50 dominant tree species from 10 typical forest sites across temperate and subtropical regions,and identified the underlying mechanisms driving these changes.For both regions combined,leaf P concentration of the 50 dominant tree species decreased(20.6%),whereas leaf N:P increased(52.0%)from 2005 to 2020.Leaf P decreased and leaf N:P increased in 67% and 69% of the tree species,respectively.The leaf N:P increase was primarily driven by the tree species in eastern subtropical forests,where global change factors and soil nutrients explained 68% of leaf N:P variation.The P limitation exhibited by tree species in eastern subtropical forest ecosystems intensified over time,and elevated temperature and CO_(2) levels,coupled with decreased soil available P concentrations,appear to be the main factors driving long-term leaf N:P increases in these forests.Investigating long-term variations in soil nutrients together with global change factors will improve our understanding of the nutrient status of forest ecosystems in the context of global change and will support effective forest ecosystem management.
基金supported by grants from the National Key Research&Development Plan(Grants Nos.2022YFF10030022022YFD1200502)+7 种基金National Natural Science Foundation of China(Grant Nos.3237269631991182)Wuhan Biological Breeding Major Project(Grant No.2022021302024852)Key Project of Hubei Hongshan Laboratory(2021hszd007)HZAU-AGIS Cooperation Fund(Grant No.SZYJY2023022)Funds for High Quality Development of Hubei Seed Industry(HBZY2023B004)Hubei Agriculture Research System(2023HBSTX4-06)Hubei Key Research&Development Plan(Grants Nos.2022BBA0066,2022BBA0062)。
文摘Photosynthesis is one the most important chemical reaction in plants,and it is the ultimate energy source of any living organisms.The light and dark reactions are two essential phases of photosynthesis.Light reaction harvests light energy to synthesize ATP and NADPH through an electron transport chain,and as well as giving out O_(2);dark reaction fixes CO_(2) into six carbon sugars by utilizing NADPH and energy from ATP.Subsequently,plants convert optical energy into chemical energy for maintaining growth and development through absorbing light energy.Here,firstly,we highlighted the biological importance of photosynthesis,and hormones and metabolites,photosynthetic and regulating enzymes,and signaling components that collectively regulate photosynthesis in tomato.Next,we reviewed the advances in tomato photosynthesis,including two aspects of genetic basis and genetic improvement.Numerous genes regulating tomato photosynthesis are gradually uncovered,and the interaction network among those genes remains to be constructed.Finally,the photosynthesis occurring in fruit of tomato and the relationship between photosynthesis in leaf and fruit were discussed.Leaves and fruits are photosynthate sources and sinks of tomato respectively,and interaction between photosynthesis in leaf and fruit exists.Additionally,future perspectives that needs to be addressed on tomato photosynthesis were proposed.
