Root distribution of three desert shrubs,Tamarix ramosissima Ledeb.,Haloxylon ammodendron(C.A.Mey.) Bunge and Reaumuria soongorica(Pall.) Maxim.was investigated under co-occurring conditions using a method for excavat...Root distribution of three desert shrubs,Tamarix ramosissima Ledeb.,Haloxylon ammodendron(C.A.Mey.) Bunge and Reaumuria soongorica(Pall.) Maxim.was investigated under co-occurring conditions using a method for excavating the whole root system.Assimilation shoot water potential and transpiration rates were monitored during the wet-dry cycle.Leaf-specific apparent hydraulic conductance and the index of water stress impact for the three species were calculated from shoot water potential and transpiration rate.The results showed that,along the soil profile,the root system of T.ramosissima mainly distributed at 50 to 310 cm interval,with an average total absorbing root-surface area of 30,249.2 cm2 per plant;the root system of H.ammodendraom distributed at 0 to 250 cm interval with an average total absorbing root-surface area of 12,847.3 cm2 per plant;the root system of R.soongorica distributed at 0-80 cm interval,with an average total absorbing root-surface area of 361.8 cm2.The root distribution shows the following:T.ramosissima uses groundwater as its main water source;H.ammodendraom uses both groundwater and rainwater;and R.soongorica uses rainwater only.During the wet-dry cycle,the hydraulic parameters of T.ramosissima showed no responses to precipitation.R.soongorica responded most significantly,and the responses of H.ammodendraom were intermediate.In conclusion,the plant response to rain events is closely related to their root distribution and plant water-use strategy.展开更多
Chalcone synthases （CHS, EC 2.3.1.74） are key enzymes that catalyze the first committed step in flavonoid biosynthesis. In this study, we isolated a chalcone synthase, named NtCHS6, from Nicotiana tabacum. This synt...Chalcone synthases （CHS, EC 2.3.1.74） are key enzymes that catalyze the first committed step in flavonoid biosynthesis. In this study, we isolated a chalcone synthase, named NtCHS6, from Nicotiana tabacum. This synthase shared high homology with the NSCHSL （Y14507） gene and contained most of the conserved active sites that are in CHS proteins. The phylogenetic analysis suggested that NtCHS6 protein shared a large genetic distance with other Solanaceae CHS proteins and was the most closely-related to an uncharacterized CHS from Solanum lycopersicum. The expression analysis indicated that NtCHS6 was abundantly expressed in leaves, especially in mature leaves. By scrutinizing its upstream promoter sequences, multiple cis-regulatory elements involved in light and drought responsive were detected. Furthermore, NtCHS6 expression decreased significantly under dark treatment and increased significantly under drought stress suggested that NtCHS6 expression exhibited both light responsiveness and drought responsiveness, and important roles in ultraviolet protection and drought tolerance. Our results might play展开更多
基金supported by National Natural Science Foundation of China (Grant No. 40725002)
文摘Root distribution of three desert shrubs,Tamarix ramosissima Ledeb.,Haloxylon ammodendron(C.A.Mey.) Bunge and Reaumuria soongorica(Pall.) Maxim.was investigated under co-occurring conditions using a method for excavating the whole root system.Assimilation shoot water potential and transpiration rates were monitored during the wet-dry cycle.Leaf-specific apparent hydraulic conductance and the index of water stress impact for the three species were calculated from shoot water potential and transpiration rate.The results showed that,along the soil profile,the root system of T.ramosissima mainly distributed at 50 to 310 cm interval,with an average total absorbing root-surface area of 30,249.2 cm2 per plant;the root system of H.ammodendraom distributed at 0 to 250 cm interval with an average total absorbing root-surface area of 12,847.3 cm2 per plant;the root system of R.soongorica distributed at 0-80 cm interval,with an average total absorbing root-surface area of 361.8 cm2.The root distribution shows the following:T.ramosissima uses groundwater as its main water source;H.ammodendraom uses both groundwater and rainwater;and R.soongorica uses rainwater only.During the wet-dry cycle,the hydraulic parameters of T.ramosissima showed no responses to precipitation.R.soongorica responded most significantly,and the responses of H.ammodendraom were intermediate.In conclusion,the plant response to rain events is closely related to their root distribution and plant water-use strategy.
基金supported by the Agricultural Science and Technology Innovation Program, China (ASTIP-TRIC01)
文摘Chalcone synthases （CHS, EC 2.3.1.74） are key enzymes that catalyze the first committed step in flavonoid biosynthesis. In this study, we isolated a chalcone synthase, named NtCHS6, from Nicotiana tabacum. This synthase shared high homology with the NSCHSL （Y14507） gene and contained most of the conserved active sites that are in CHS proteins. The phylogenetic analysis suggested that NtCHS6 protein shared a large genetic distance with other Solanaceae CHS proteins and was the most closely-related to an uncharacterized CHS from Solanum lycopersicum. The expression analysis indicated that NtCHS6 was abundantly expressed in leaves, especially in mature leaves. By scrutinizing its upstream promoter sequences, multiple cis-regulatory elements involved in light and drought responsive were detected. Furthermore, NtCHS6 expression decreased significantly under dark treatment and increased significantly under drought stress suggested that NtCHS6 expression exhibited both light responsiveness and drought responsiveness, and important roles in ultraviolet protection and drought tolerance. Our results might play
