[Objectives]To explore the genetic mechanism that leaf length and leaf width in bitter gourd has realistic guiding significance for formulating breeding strategy.[Methods]In this paper,the cross was made between CN19-...[Objectives]To explore the genetic mechanism that leaf length and leaf width in bitter gourd has realistic guiding significance for formulating breeding strategy.[Methods]In this paper,the cross was made between CN19-1 and Thai4-6 and an F2segregation population was constructed.The genetic characteristics of leaf length and leaf width were analyzed by employing the major gene plus polygene mixed genetic model.[Results]The data analysis results demonstrated that the leaf length and leaf width of the F_(2) segregation population showed continuous distribution.The optimum model for leaf length genetic analysis was A-1,the additive effect value of the major gene was 3.1766,the dominant effect value was-3.1766,and the heritability was 74.51%.The optimum model for leaf width genetic analysis was B-1.The additive effect values of 2 pairs of major genes controlling the leaf width were 3.1917 and 0.9623,respectively.The dominant effect values were-1.3135 and-0.3463,respectively.The additive×additive interaction effect was-0.3591,the additive×dominant interaction effect was-0.2570,the dominant×additive interaction effect was 1.9916,the dominant×dominant interaction effect was 1.9686.The heritability of major genes was 96.67%.[Conclusions]This study is expected to provide a theoretical basis for the breeding of bitter gourd.展开更多
The width of rice leaves determines the size of the photosynthetic area.Optimizing rice leaf width can improve the photosynthetic rate,thereby increasing rice yield.In this study,a genome-wide association study(GWAS)w...The width of rice leaves determines the size of the photosynthetic area.Optimizing rice leaf width can improve the photosynthetic rate,thereby increasing rice yield.In this study,a genome-wide association study(GWAS)was conducted by 225 rice germplasm resources to explore the genetic basis of rice flag leaf width(FLW).We identified nine QTLs associated with FLW(qFLWs),with phenotypic contribution rates ranging from 3.17%to 14.37%.Near-isogenic lines(NILs)were developed for fine-mapping of qFLW11,and the function of FLW11 was further verified.We narrowed down q FLW11 to an 87-kb interval,which contains five genes.展开更多
The present study was conducted to identify quantitative trait loci （QTLs） for leaf size traits in IR64 introgression lines （INLs）. For this purpose, selected F2 populations derived from crosses between recurrent ...The present study was conducted to identify quantitative trait loci （QTLs） for leaf size traits in IR64 introgression lines （INLs）. For this purpose, selected F2 populations derived from crosses between recurrent parent IR64 and its derived INLs, unique for leaf length and leaf width, were used to confirm QTLs. A total of eight QTLs, mapped on three chromosomes, were identified for the four leaf size traits in six F2 populations. A QTL for leaf length, qLLnpt-1, in HKL69 was identified around simple sequence repeat （SSR） marker RM3709 on chromosome 1. Two QTLs for flag leaf length, qFLLnpt-2 and qFLLnpt-4, in HFG39 were indentified on chromosomes 2 and 4, respectively. For flag leaf width, a QTL, qFLWnpt-4, in HFG39 was identified around RM17483 on chromosome 4. While another QTL for flag leaf width, qFLWnpt-1, in HFG27 was identified around RM3252 on chromosome 1. A QTL for leaf width, qLWnpt-2, in HKL75 was identified around RM7451 on chromosome 2. For leaf width, two QTLs, qLWnpt-4a, qLWnpt-4b, in HKL48 and HKL99 were identified around RM7208 and RM6909, respectively on chromosome 4. Results from this study suggest the possibilities to use marker-assisted selection and pyramiding these QTLs to improve rice water productivity.展开更多
Aerial organs in rice,including leaves,stems,and grains,are crucial for photosynthesis,lodging resistance,and yield.Therefore,an in-depth study on the development of these organs can lay a foundation for achieving hig...Aerial organs in rice,including leaves,stems,and grains,are crucial for photosynthesis,lodging resistance,and yield.Therefore,an in-depth study on the development of these organs can lay a foundation for achieving high and stable rice yields.In this study,we isolated a novel slender aerial organ mutant sao,which is characterized by a significant reduction in the width of leaves,stems,and grains.Histological analysis revealed that the slender phenotype of aerial organs in sao is caused by impaired cell proliferation and elongation.展开更多
Convenient and effective methods to determine seasonal changes in individual leaf area (LA) and leaf mass (LM) of plants are useful in research on plant physiology and forest ecology. However, practical methods for es...Convenient and effective methods to determine seasonal changes in individual leaf area (LA) and leaf mass (LM) of plants are useful in research on plant physiology and forest ecology. However, practical methods for estimating LA and LM of elm (Ulmus japonica) leaves in different periods have rarely been reported. We collected sample elm leaves in June, July and September. Then, we developed allometric models relating LA, LM and leaf parameters, such as leaf length (L) and width (W) or the product of L and W (LW). Our objective was to find optimal allometric models for conveniently and effectively estimating LA and LM of elm leaves in different periods. LA and LM were significantly correlated with leaf parameters (P < 0.05), and allometric models with LW as an independent variable were best for estimating LA and LM in each period. A linear model was separately developed to predict LA of elm leaves in June, July and September, and it yielded high accuracies of 93, 96 and 96%, respectively. Similarly, a specific allometric model for predicting LM was developed separately in three periods, and the optimal model form in both June and July was a power model, but the linear model was optimal for September. The accuracies of the allometric models in predicting LM were 88, 83 and 84% for June, July and September, respectively. The error caused by ignoring seasonal variation of allometric models in predicting LA and LM in the three periods were 1-4 and 16-59%, respectively.展开更多
Objective Radiotherapy combined with conservative surgery plays an important role in the treatment of early-stage breast cancer. Volumetric modulated arc therapy(VMAT) has been introduced into clinical practice. The p...Objective Radiotherapy combined with conservative surgery plays an important role in the treatment of early-stage breast cancer. Volumetric modulated arc therapy(VMAT) has been introduced into clinical practice. The purpose of this study was to investigate the dosimetric effects of different multileaf collimators(MLC) on VMAT radiotherapy plans for treating breast cancer.Methods Fifteen breast cancer patients who were treated using a conventional technique in our department were selected to participate in this retrospective analysis. VMAT plans based on three types of Elekta MLCs [Beam Modulator(BM) with 4-mm leaf width, Agility with 5-mm leaf width and MLCi2 with 10-mm leaf width] were independently generated for each patient. Plan comparisons were performed based on dose-volume histogram(DVH) analysis including dosimetric parameters such as the homogeneity index(HI), conformity index(CI), Dmax, Dmin, and Dmean for the planning treatment volume(PTV), in addition to dose-volume parameters for the organs at risk(OARs). The delivery efficiency of the three types of MLCs was compared in terms of the beam delivery time and the monitor units(MUs) per fraction for each plan. Results Both target uniformity and conformity were improved in plans for Agility and BM MLC compared with the plan using MLCi2. The mean HI decreased from 1.14 for MLCi2 to 1.13 for BM and 1.10 for Agility, while the mean CI increased from 0.68 for MLCi2 to 0.73 for BM and 0.75 for Agility. Furthermore, at both low and high dose levels, smaller volumes of ipsilateral lung, heart, contralateral lung, and breast were irradiated with Agility MLC than with the other two types of MLCs. The delivery time with Agility MLC was reduced by 10.8% and 32.1%, respectively, compared with that for MLCi2 and BM.Conclusion Our results indicate that the Agility MLC exhibits a dosimetric advantage and a significant improvement in delivery efficiency for the treatment of breast cancer using VMAT.展开更多
The leaf morphological and stomatal characteristics of four paper birch (Betula papyrifera Marsh) populations, grown at four treatment conditions of carbon dioxide [CO2] and soil water levels were investigated to dete...The leaf morphological and stomatal characteristics of four paper birch (Betula papyrifera Marsh) populations, grown at four treatment conditions of carbon dioxide [CO2] and soil water levels were investigated to determine whether future increases in atmospheric [CO2] and water deficit affected the leaf characteristics. The populations from Cussion Lake, Little Oliver, Skimikin and Wayerton were grown for 12 weeks under ambient (360 ppm) and elevated (720 ppm) [CO2] at both high and low water levels. The populations significantly differed in leaf area and stomatal characteristics due to the interaction effects of [CO2], water levels and population differences. Most leaf morphological characteristics and stomatal density varied due to the effects of [CO2] and/or populations, but not due to the effect of water levels. Although elevated [CO2] alone barely affected stomatal area of the birch populations, simultaneous elevated [CO2] at both water levels had stimulated stomatal characteristics within and among the populations. Overall, elevated [CO2] reduced leaf area and increased stomatal density;and low water level resulted in smaller stomatal area, pore area and guard cell width. However, the populations responded differently to an increase in [CO2] and water levels. All populations showed plastic responses with respect to [CO2] and water levels either by decreasing stomatal area under low water level or by increasing stomatal density under elevated [CO2]. Hence, integration between and within leaf characteristics had helped paper birch populations maintain balance between [CO2] gain and water loss.展开更多
基金Hainan Science and Technology Project(ZDYF2020229ZDKJ2021010)Scientific Research Program of Hainan Key Laboratory of Vegetable Biology and Hainan Key Laboratory for Quality Regulation of Tropical Horticultural Crops(HNZDSYS(YY)-03)。
文摘[Objectives]To explore the genetic mechanism that leaf length and leaf width in bitter gourd has realistic guiding significance for formulating breeding strategy.[Methods]In this paper,the cross was made between CN19-1 and Thai4-6 and an F2segregation population was constructed.The genetic characteristics of leaf length and leaf width were analyzed by employing the major gene plus polygene mixed genetic model.[Results]The data analysis results demonstrated that the leaf length and leaf width of the F_(2) segregation population showed continuous distribution.The optimum model for leaf length genetic analysis was A-1,the additive effect value of the major gene was 3.1766,the dominant effect value was-3.1766,and the heritability was 74.51%.The optimum model for leaf width genetic analysis was B-1.The additive effect values of 2 pairs of major genes controlling the leaf width were 3.1917 and 0.9623,respectively.The dominant effect values were-1.3135 and-0.3463,respectively.The additive×additive interaction effect was-0.3591,the additive×dominant interaction effect was-0.2570,the dominant×additive interaction effect was 1.9916,the dominant×dominant interaction effect was 1.9686.The heritability of major genes was 96.67%.[Conclusions]This study is expected to provide a theoretical basis for the breeding of bitter gourd.
基金supported by the Zhejiang Provincial Natural Science Foundation,China(Grant No.LD24C130001)the National Natural Science Foundation of China(Grant Nos.W2412006 and 32372125)+3 种基金the Hainan Provincial Natural Science Foundation,China(Grant Nos.GHYF2025029 and YBXM2422)the Innovation Platform for Academicians of Hainan Province,China(Grant No.YSPTZX202502)the National Modern Agricultural Industry Technology System Project,China(Grant No.CARS-01-18)the Special Support Program of Chinese Academy of Agricultural Sciences(Grant Nos.NKYCLJ-C-2021-015 and CAAS-ZDRW202401)。
文摘The width of rice leaves determines the size of the photosynthetic area.Optimizing rice leaf width can improve the photosynthetic rate,thereby increasing rice yield.In this study,a genome-wide association study(GWAS)was conducted by 225 rice germplasm resources to explore the genetic basis of rice flag leaf width(FLW).We identified nine QTLs associated with FLW(qFLWs),with phenotypic contribution rates ranging from 3.17%to 14.37%.Near-isogenic lines(NILs)were developed for fine-mapping of qFLW11,and the function of FLW11 was further verified.We narrowed down q FLW11 to an 87-kb interval,which contains five genes.
文摘The present study was conducted to identify quantitative trait loci （QTLs） for leaf size traits in IR64 introgression lines （INLs）. For this purpose, selected F2 populations derived from crosses between recurrent parent IR64 and its derived INLs, unique for leaf length and leaf width, were used to confirm QTLs. A total of eight QTLs, mapped on three chromosomes, were identified for the four leaf size traits in six F2 populations. A QTL for leaf length, qLLnpt-1, in HKL69 was identified around simple sequence repeat （SSR） marker RM3709 on chromosome 1. Two QTLs for flag leaf length, qFLLnpt-2 and qFLLnpt-4, in HFG39 were indentified on chromosomes 2 and 4, respectively. For flag leaf width, a QTL, qFLWnpt-4, in HFG39 was identified around RM17483 on chromosome 4. While another QTL for flag leaf width, qFLWnpt-1, in HFG27 was identified around RM3252 on chromosome 1. A QTL for leaf width, qLWnpt-2, in HKL75 was identified around RM7451 on chromosome 2. For leaf width, two QTLs, qLWnpt-4a, qLWnpt-4b, in HKL48 and HKL99 were identified around RM7208 and RM6909, respectively on chromosome 4. Results from this study suggest the possibilities to use marker-assisted selection and pyramiding these QTLs to improve rice water productivity.
基金supported by the Sichuan Science and Technology Program,China(Grant No.2024JDRC0064)the Chongqing Talent Program Foundation,China(Grant No.cstc2024ycjh-bgzxm0063)+1 种基金the National Natural Science Foundation of China(Grant No.32470354)the Sichuan Science and Technology Innovation and Entrepreneurship Seedling Foundation,China(Grant No.2024JDRC0064).
文摘Aerial organs in rice,including leaves,stems,and grains,are crucial for photosynthesis,lodging resistance,and yield.Therefore,an in-depth study on the development of these organs can lay a foundation for achieving high and stable rice yields.In this study,we isolated a novel slender aerial organ mutant sao,which is characterized by a significant reduction in the width of leaves,stems,and grains.Histological analysis revealed that the slender phenotype of aerial organs in sao is caused by impaired cell proliferation and elongation.
基金financially supported by the National Natural Science Foundation of China(No.31600587)
文摘Convenient and effective methods to determine seasonal changes in individual leaf area (LA) and leaf mass (LM) of plants are useful in research on plant physiology and forest ecology. However, practical methods for estimating LA and LM of elm (Ulmus japonica) leaves in different periods have rarely been reported. We collected sample elm leaves in June, July and September. Then, we developed allometric models relating LA, LM and leaf parameters, such as leaf length (L) and width (W) or the product of L and W (LW). Our objective was to find optimal allometric models for conveniently and effectively estimating LA and LM of elm leaves in different periods. LA and LM were significantly correlated with leaf parameters (P < 0.05), and allometric models with LW as an independent variable were best for estimating LA and LM in each period. A linear model was separately developed to predict LA of elm leaves in June, July and September, and it yielded high accuracies of 93, 96 and 96%, respectively. Similarly, a specific allometric model for predicting LM was developed separately in three periods, and the optimal model form in both June and July was a power model, but the linear model was optimal for September. The accuracies of the allometric models in predicting LM were 88, 83 and 84% for June, July and September, respectively. The error caused by ignoring seasonal variation of allometric models in predicting LA and LM in the three periods were 1-4 and 16-59%, respectively.
文摘Objective Radiotherapy combined with conservative surgery plays an important role in the treatment of early-stage breast cancer. Volumetric modulated arc therapy(VMAT) has been introduced into clinical practice. The purpose of this study was to investigate the dosimetric effects of different multileaf collimators(MLC) on VMAT radiotherapy plans for treating breast cancer.Methods Fifteen breast cancer patients who were treated using a conventional technique in our department were selected to participate in this retrospective analysis. VMAT plans based on three types of Elekta MLCs [Beam Modulator(BM) with 4-mm leaf width, Agility with 5-mm leaf width and MLCi2 with 10-mm leaf width] were independently generated for each patient. Plan comparisons were performed based on dose-volume histogram(DVH) analysis including dosimetric parameters such as the homogeneity index(HI), conformity index(CI), Dmax, Dmin, and Dmean for the planning treatment volume(PTV), in addition to dose-volume parameters for the organs at risk(OARs). The delivery efficiency of the three types of MLCs was compared in terms of the beam delivery time and the monitor units(MUs) per fraction for each plan. Results Both target uniformity and conformity were improved in plans for Agility and BM MLC compared with the plan using MLCi2. The mean HI decreased from 1.14 for MLCi2 to 1.13 for BM and 1.10 for Agility, while the mean CI increased from 0.68 for MLCi2 to 0.73 for BM and 0.75 for Agility. Furthermore, at both low and high dose levels, smaller volumes of ipsilateral lung, heart, contralateral lung, and breast were irradiated with Agility MLC than with the other two types of MLCs. The delivery time with Agility MLC was reduced by 10.8% and 32.1%, respectively, compared with that for MLCi2 and BM.Conclusion Our results indicate that the Agility MLC exhibits a dosimetric advantage and a significant improvement in delivery efficiency for the treatment of breast cancer using VMAT.
文摘The leaf morphological and stomatal characteristics of four paper birch (Betula papyrifera Marsh) populations, grown at four treatment conditions of carbon dioxide [CO2] and soil water levels were investigated to determine whether future increases in atmospheric [CO2] and water deficit affected the leaf characteristics. The populations from Cussion Lake, Little Oliver, Skimikin and Wayerton were grown for 12 weeks under ambient (360 ppm) and elevated (720 ppm) [CO2] at both high and low water levels. The populations significantly differed in leaf area and stomatal characteristics due to the interaction effects of [CO2], water levels and population differences. Most leaf morphological characteristics and stomatal density varied due to the effects of [CO2] and/or populations, but not due to the effect of water levels. Although elevated [CO2] alone barely affected stomatal area of the birch populations, simultaneous elevated [CO2] at both water levels had stimulated stomatal characteristics within and among the populations. Overall, elevated [CO2] reduced leaf area and increased stomatal density;and low water level resulted in smaller stomatal area, pore area and guard cell width. However, the populations responded differently to an increase in [CO2] and water levels. All populations showed plastic responses with respect to [CO2] and water levels either by decreasing stomatal area under low water level or by increasing stomatal density under elevated [CO2]. Hence, integration between and within leaf characteristics had helped paper birch populations maintain balance between [CO2] gain and water loss.
