We used the leaf blade of rice (cultivars were Nonghu 6, Sugeng 2, Huyou 2 and Hanfeng) as initial material for protoplast culture, and a great number of regenerated plants were obtained. Rice seeds were sterilized an...We used the leaf blade of rice (cultivars were Nonghu 6, Sugeng 2, Huyou 2 and Hanfeng) as initial material for protoplast culture, and a great number of regenerated plants were obtained. Rice seeds were sterilized and germinated. The immature leaves were cut into 3-5 mm pieces when the third or forth leaf appeared. Leaf pieces were inoculated on MS medium with 2,4-D 4 mg/1, NAA 2mg/1 and IAA Img/1. After 2 wk culture, calli were induced and subcultured once or twice for multiplication. 3-5 g calli were transferred to the modified MS liquid medium with 2,4-D 2 mg/1 and KT 0.5mg/1 for suspension culture. Embryogenic cell suspension was established after 2 mo culture. The effect of the growth period of suspension cells on the展开更多
Main progress of transgenic techniques in apple made in recent years was summarized, and related research achievements in various aspects like transformation method, regenerating ability of explants, strain type and i...Main progress of transgenic techniques in apple made in recent years was summarized, and related research achievements in various aspects like transformation method, regenerating ability of explants, strain type and infection conditions of Agrobacterium were reviewed. On this basis, the current bottlenecks in transgenic techniques in apple were put forward.展开更多
文摘We used the leaf blade of rice (cultivars were Nonghu 6, Sugeng 2, Huyou 2 and Hanfeng) as initial material for protoplast culture, and a great number of regenerated plants were obtained. Rice seeds were sterilized and germinated. The immature leaves were cut into 3-5 mm pieces when the third or forth leaf appeared. Leaf pieces were inoculated on MS medium with 2,4-D 4 mg/1, NAA 2mg/1 and IAA Img/1. After 2 wk culture, calli were induced and subcultured once or twice for multiplication. 3-5 g calli were transferred to the modified MS liquid medium with 2,4-D 2 mg/1 and KT 0.5mg/1 for suspension culture. Embryogenic cell suspension was established after 2 mo culture. The effect of the growth period of suspension cells on the
基金Supported by Natural Science Foundation of Hebei Province （C2004000368, C2005000192） Doctoral Fund of Henan Institute of Science and Technology （20070025）.
文摘Main progress of transgenic techniques in apple made in recent years was summarized, and related research achievements in various aspects like transformation method, regenerating ability of explants, strain type and infection conditions of Agrobacterium were reviewed. On this basis, the current bottlenecks in transgenic techniques in apple were put forward.
