A successful simulation still requires the user to have good simulation knowledge and well developed modeling skills despite a large number of simulation software products available to users. This paper presents the d...A successful simulation still requires the user to have good simulation knowledge and well developed modeling skills despite a large number of simulation software products available to users. This paper presents the design principles and implementation of a layered modeling system known as General-Purpose user-defined Modeling System (GPMS) which provides the user with multiple accesses to build a simulation model at three different levels of knowledge and skills. It does this by purpose-designed GPMS simulation language, which is briefly described in this paper.展开更多
The evolution of two spanwise-aligned low-speed streaks in a wall turbulent flow, triggered by the instability of the subharmonic varicose （SV） mode, is studied by a direct numerical simulation （DNS） method in a s...The evolution of two spanwise-aligned low-speed streaks in a wall turbulent flow, triggered by the instability of the subharmonic varicose （SV） mode, is studied by a direct numerical simulation （DNS） method in a small spatial-periodic channel. The results show that the SV low-speed streaks are self-sustained at the early stage, and then transform into subharmonic sinuous （SS） low-speed streaks. Initially, the streamwise vortex sheets are formed by shearing, and then evolve into zigzag vortex sheets due to the mutual induction. As the intensification of the SV low-speed streaks becomes prominent, the tilted streamwise vortex tubes and the V-like streamwise vortex tubes can be formed simultaneously by increasing ＋~. When the SV low-speed streaks break down, new zigzag streamwise vortices will be generated, thus giving birth to the next sustaining cycle of the SV low-speed streaks. When the second breakdown happens, new secondary V-like streamwise vortices instead of zigzag streamwise vortices will be generated. Because of the sweep motion of the fluid induced by the secondary V-like streamwise vortices, each decayed low-speed streak can be divided into two parts, and each part combines with the part of another streak, finally leading to the formation of SS low-speed streaks.展开更多
Layered double hydroxides （LDHs） are effective molecular carriers in cytological research, gene therapy, and transgenic applications. Herein, we investigated the internalization behavior of the LDH-DNA biocon- jugat...Layered double hydroxides （LDHs） are effective molecular carriers in cytological research, gene therapy, and transgenic applications. Herein, we investigated the internalization behavior of the LDH-DNA biocon- jugates via a microscopic approach and analyzed the internalization pathway by dissipative particle dynamics （DPD） simulations. We experimentally found that LDH can efficiently carry DNA into the nucleus of cell in BY-2 suspension cells. Furthermore, atomic force microscopy and X-ray diffraction anal- ysis demonstrated that the LDH-DNA bioconjugates mainly exist as a DNA-LDH-DNA sandwich complex, while the LDH-DNA-LDH sandwich complex and DNA-LDH complex cannot be excluded. The DPD simu- lations further indicated that only the DNA-LDH-DNA sandwich structure could penetrate the plasma membrane （PM）, while PM is impermeable to the LDH-DNA-LDH sandwich complex and the DNA-LDH complex. This work provides novel perspective for understanding the membrane penetration mechanism of LDH nano-sheets and new insights into the design of novel molecular delivery systems.展开更多
文摘A successful simulation still requires the user to have good simulation knowledge and well developed modeling skills despite a large number of simulation software products available to users. This paper presents the design principles and implementation of a layered modeling system known as General-Purpose user-defined Modeling System (GPMS) which provides the user with multiple accesses to build a simulation model at three different levels of knowledge and skills. It does this by purpose-designed GPMS simulation language, which is briefly described in this paper.
基金supported by the National Natural Science Foundation of China(Nos.11372140 and11202102)the Innovation Project for College Graduates of Jiangsu Province(No.CXZZ13-0189)
文摘The evolution of two spanwise-aligned low-speed streaks in a wall turbulent flow, triggered by the instability of the subharmonic varicose （SV） mode, is studied by a direct numerical simulation （DNS） method in a small spatial-periodic channel. The results show that the SV low-speed streaks are self-sustained at the early stage, and then transform into subharmonic sinuous （SS） low-speed streaks. Initially, the streamwise vortex sheets are formed by shearing, and then evolve into zigzag vortex sheets due to the mutual induction. As the intensification of the SV low-speed streaks becomes prominent, the tilted streamwise vortex tubes and the V-like streamwise vortex tubes can be formed simultaneously by increasing ＋~. When the SV low-speed streaks break down, new zigzag streamwise vortices will be generated, thus giving birth to the next sustaining cycle of the SV low-speed streaks. When the second breakdown happens, new secondary V-like streamwise vortices instead of zigzag streamwise vortices will be generated. Because of the sweep motion of the fluid induced by the secondary V-like streamwise vortices, each decayed low-speed streak can be divided into two parts, and each part combines with the part of another streak, finally leading to the formation of SS low-speed streaks.
基金supported by the Fundamental Research Funds for the Central Universities (2016JX01, BLX2015-01)the National Natural Science Foundation of China (31671489, 31601149 and 31271433)+2 种基金the Beijing Nova Programme (Z131109000413013)the Special Program for Applied Research on Super Computation of the NSFC-Guangdong Joint Fundthe Chemical Grid Program and Excellent Talent of Beijing University of Chemical Technology
文摘Layered double hydroxides （LDHs） are effective molecular carriers in cytological research, gene therapy, and transgenic applications. Herein, we investigated the internalization behavior of the LDH-DNA biocon- jugates via a microscopic approach and analyzed the internalization pathway by dissipative particle dynamics （DPD） simulations. We experimentally found that LDH can efficiently carry DNA into the nucleus of cell in BY-2 suspension cells. Furthermore, atomic force microscopy and X-ray diffraction anal- ysis demonstrated that the LDH-DNA bioconjugates mainly exist as a DNA-LDH-DNA sandwich complex, while the LDH-DNA-LDH sandwich complex and DNA-LDH complex cannot be excluded. The DPD simu- lations further indicated that only the DNA-LDH-DNA sandwich structure could penetrate the plasma membrane （PM）, while PM is impermeable to the LDH-DNA-LDH sandwich complex and the DNA-LDH complex. This work provides novel perspective for understanding the membrane penetration mechanism of LDH nano-sheets and new insights into the design of novel molecular delivery systems.
