Larval settlement and metamorphosis are important developmental events in marine invertebrates.Some neuropeptides may play a key role in these processes.In this study,we revealed the expression characteristics of myoi...Larval settlement and metamorphosis are important developmental events in marine invertebrates.Some neuropeptides may play a key role in these processes.In this study,we revealed the expression characteristics of myoinhibitory peptide(MIP)in the embryos and larvae of the echiuran worm Urechis unicinctus using RT-qPCR,in situ hybridization and immunofluorescence techniques,and found its location in the top cells of the upper hemisphere was conservative in larvae.Furthermore,we used the active MIP peptide to verify its role in inducing the larval settlement of U.unicinctus,and confirmed that MIP can trigger an obvious larval settlement when 10μmol L^(−1) or 15μmol L^(−1) MIP was added in the seawater where early-segmentation larvae were cultured.Our finding indicated that neuropeptide MIP participates in the larval settlement in U.unicinctus.展开更多
基金This work was support by the Fundamental Research Funds for the Central Universities(Nos.202064006,202113045)the National Natural Science Foundation of China(Nos.31572601,42176122)+1 种基金the Key Research&Development Project of Hainan Province(No.ZDYF2021XD NY180)the Postdoctoral Applied Research Project of Qingdao.
文摘Larval settlement and metamorphosis are important developmental events in marine invertebrates.Some neuropeptides may play a key role in these processes.In this study,we revealed the expression characteristics of myoinhibitory peptide(MIP)in the embryos and larvae of the echiuran worm Urechis unicinctus using RT-qPCR,in situ hybridization and immunofluorescence techniques,and found its location in the top cells of the upper hemisphere was conservative in larvae.Furthermore,we used the active MIP peptide to verify its role in inducing the larval settlement of U.unicinctus,and confirmed that MIP can trigger an obvious larval settlement when 10μmol L^(−1) or 15μmol L^(−1) MIP was added in the seawater where early-segmentation larvae were cultured.Our finding indicated that neuropeptide MIP participates in the larval settlement in U.unicinctus.
