In order to study the relationship between landmarks and spatial memory in short-nosed fruit bat, Cynopterus sphinx （Megachiroptera, Pteropodidae）, we simulated a foraging environment in the laboratory. Different la...In order to study the relationship between landmarks and spatial memory in short-nosed fruit bat, Cynopterus sphinx （Megachiroptera, Pteropodidae）, we simulated a foraging environment in the laboratory. Different landmarks were placed to gauge the spatial memory of C. sphinx. We changed the number of landmarks every day with 0 landmarks again on the fifth day （from 0, 2, 4, 8 to 0）. Individuals from the control group were exposed to the identical artificial foraging environment, but without landmarks. The results indicated that there was significant correlation between the time of the first foraging and the experimental days in both groups （Pearson Correlation： experimental group： r=-0.593, P〈0.01; control group： r=-0.581, P〈0.01）. There was no significant correlation between the success rates of foraging and the experimental days in experimental groups （Pearson Correlation： r=0.177, P〉0.05）, but there was significant correlation between the success rates of foraging and the experimental days in the control groups （Pearson Correlation： r=0.445, P〈0.05）. There was no significant difference for the first foraging time between experimental and control groups （GLM： F0.05,1=4.703, P〉0.05）; also, there was no significant difference in success rates of foraging between these two groups （GLM： F0.05,1=0.849,P〉0.05）. The results of our experiment suggest that spatial memory in C. sphinx was formed gradually and that the placed landmarks appeared to have no discernable effects on the memory of the foraging space.展开更多
目的评估神经刺激器引导下1.3%布比卡因脂质体在小鼠坐骨神经联合股神经阻滞(SFNB)动物模型的制备效果。方法选择雄性C57BL/6J小鼠49只,18月龄,体重28~32 g。实验一:选择34只小鼠进行神经阻滞效果评估,采用随机数字表法分为两组:神经刺...目的评估神经刺激器引导下1.3%布比卡因脂质体在小鼠坐骨神经联合股神经阻滞(SFNB)动物模型的制备效果。方法选择雄性C57BL/6J小鼠49只,18月龄,体重28~32 g。实验一:选择34只小鼠进行神经阻滞效果评估,采用随机数字表法分为两组:神经刺激器引导组(S组)和解剖标志定位组(C组),每组17只。S组采用神经刺激器引导行SFNB,C组采用解剖标志定位行SFNB。采用机械刺激缩足反应阈值(PWMT)和4级评分法,分别于阻滞前1 d和阻滞后2、6、24、36、48 h评估两组阻滞成功的小鼠感觉和运动阻滞程度。实验二:选择剩余15只小鼠进行神经染色研究,以上述方法于每只小鼠两侧分别采用神经刺激器引导(S1组)或解剖标志定位(C1组)行坐骨神经和股神经亚甲蓝染色,评估阻滞后小鼠神经染色分级和神经Ⅲ级染色长度。结果实验一:与C组比较,S组阻滞成功率明显升高[15只(88.2%)vs.9只(52.9%),P<0.05],阻滞后6、24 h PWMT明显升高(P<0.05)。与阻滞前1 d比较,阻滞后2、6、24、36 h S组PMWT明显升高,阻滞后2 h C组PMWT明显升高(P<0.05)。实验二:与C1组比较,S1组坐骨神经和股神经染色分级明显升高,Ⅲ级染色长度明显延长(P<0.05)。结论与解剖标志定位比较,神经刺激器引导下1.3%布比卡因脂质体SFNB的小鼠模型具有阻滞成功率高、感觉阻滞持续时间长、神经染色均匀的优势,是一种较为理想的坐骨神经联合股神经阻滞的实验动物模型。展开更多
基金supported by the National Natural Science Foundation of China(NSFC,No30800102)Natural Science Foundation of Hainan Province(309026)
文摘In order to study the relationship between landmarks and spatial memory in short-nosed fruit bat, Cynopterus sphinx （Megachiroptera, Pteropodidae）, we simulated a foraging environment in the laboratory. Different landmarks were placed to gauge the spatial memory of C. sphinx. We changed the number of landmarks every day with 0 landmarks again on the fifth day （from 0, 2, 4, 8 to 0）. Individuals from the control group were exposed to the identical artificial foraging environment, but without landmarks. The results indicated that there was significant correlation between the time of the first foraging and the experimental days in both groups （Pearson Correlation： experimental group： r=-0.593, P〈0.01; control group： r=-0.581, P〈0.01）. There was no significant correlation between the success rates of foraging and the experimental days in experimental groups （Pearson Correlation： r=0.177, P〉0.05）, but there was significant correlation between the success rates of foraging and the experimental days in the control groups （Pearson Correlation： r=0.445, P〈0.05）. There was no significant difference for the first foraging time between experimental and control groups （GLM： F0.05,1=4.703, P〉0.05）; also, there was no significant difference in success rates of foraging between these two groups （GLM： F0.05,1=0.849,P〉0.05）. The results of our experiment suggest that spatial memory in C. sphinx was formed gradually and that the placed landmarks appeared to have no discernable effects on the memory of the foraging space.
文摘目的评估神经刺激器引导下1.3%布比卡因脂质体在小鼠坐骨神经联合股神经阻滞(SFNB)动物模型的制备效果。方法选择雄性C57BL/6J小鼠49只,18月龄,体重28~32 g。实验一:选择34只小鼠进行神经阻滞效果评估,采用随机数字表法分为两组:神经刺激器引导组(S组)和解剖标志定位组(C组),每组17只。S组采用神经刺激器引导行SFNB,C组采用解剖标志定位行SFNB。采用机械刺激缩足反应阈值(PWMT)和4级评分法,分别于阻滞前1 d和阻滞后2、6、24、36、48 h评估两组阻滞成功的小鼠感觉和运动阻滞程度。实验二:选择剩余15只小鼠进行神经染色研究,以上述方法于每只小鼠两侧分别采用神经刺激器引导(S1组)或解剖标志定位(C1组)行坐骨神经和股神经亚甲蓝染色,评估阻滞后小鼠神经染色分级和神经Ⅲ级染色长度。结果实验一:与C组比较,S组阻滞成功率明显升高[15只(88.2%)vs.9只(52.9%),P<0.05],阻滞后6、24 h PWMT明显升高(P<0.05)。与阻滞前1 d比较,阻滞后2、6、24、36 h S组PMWT明显升高,阻滞后2 h C组PMWT明显升高(P<0.05)。实验二:与C1组比较,S1组坐骨神经和股神经染色分级明显升高,Ⅲ级染色长度明显延长(P<0.05)。结论与解剖标志定位比较,神经刺激器引导下1.3%布比卡因脂质体SFNB的小鼠模型具有阻滞成功率高、感觉阻滞持续时间长、神经染色均匀的优势,是一种较为理想的坐骨神经联合股神经阻滞的实验动物模型。
