[Objective] This study was to explore the effect of three precursors on the accumulation of principal volatile oil constituents in tissue culture plantlets of Atractylodes lancea （Thunb.） DC. with the aim to provide...[Objective] This study was to explore the effect of three precursors on the accumulation of principal volatile oil constituents in tissue culture plantlets of Atractylodes lancea （Thunb.） DC. with the aim to provide references for the improvement of artificial cultivated A. lancea quality. [Method] Three precursors were added into the MS rooting medium for A. lancea tissue culture plantlets and the volatile oil was extracted by ultrasonication after cultured for several days. The content of atractylon, atractylol, β-eucalyptol and atractydin in the volatile oil were determined by using gas chromatography method. [Result] The addition of xylose, isoprene and tetrahydrofuran impacted the growth indicators, yield of volatile oil and relative percentage content of the four constituents of A. lancea tissue culture plantlets. In the 6 g/L xylose optimized medium, the atractylon and β-eucalyptol content reached up to 4.23% and 56.34%, respectively, 1.41% and 1.66% higher than the control; although the addition of isoprene into medium raised the atractylon content, the accumulation of total volatile oil was inhibited that it decreased by 23.67%, 31.06% and 7.10% to the control; for the tetrahydrofuran optimized medium, the content of atractylon, atractylol and atractydin all increased, and the total volatile oil content increased by 49.97% to the control when the concentration of tetrahydrofuran was 0.07 g/L. [Conclusion] The addition of xylose and tetrahydrofuran promoted the accumulation of principal constituents of the volatile oil, whereas the addition of isoprene inhibited the accumulation.展开更多
[Objective] To examine the effect of endophytic fungi ALl2 （Gilmaniella sp.） on metabolites distribution in organs of Atractylodes lancea. [Method] Endophytic fungi ALl2 was inoculated on Atraetylodes lancea plantle...[Objective] To examine the effect of endophytic fungi ALl2 （Gilmaniella sp.） on metabolites distribution in organs of Atractylodes lancea. [Method] Endophytic fungi ALl2 was inoculated on Atraetylodes lancea plantlets in tissue culture, and the distribution of cellulose, hemicellulose, lignin, soluble sugar in leaves and roots of the inoculated group were detected. The weight of leaves and roots were compared. Gas Chromatography was used to analyze the volatile oil components. [Result] Compared with the control group, the average fresh weight and dry weight of leaves and roots of A. lancea which had been symbiosed with ALl2 increased significantly. The content of lignin and soluble sugar increased in the leaves of the inoculated group, and the content of cellulose, hemicellulose, lignin, soluble sugar and volatile oil also increased in roots. [Conclusion] The results indicate that symbiosed with ALl2 is benefit for the development of A. lancea roots and can promote the transfer and accumulation of the medicinal components to the roots.展开更多
The rhizome of Atractylodes Iancen(A.lancea)(Thunb.) DC.(AL)is extensively used in Chinese,Thai,and Japanese traditional medicines as crude extracts/decoctions or a component in various herbal formulations.Various pha...The rhizome of Atractylodes Iancen(A.lancea)(Thunb.) DC.(AL)is extensively used in Chinese,Thai,and Japanese traditional medicines as crude extracts/decoctions or a component in various herbal formulations.Various pharmacological activities of Al.and its major constituents have been demonstrated in ritro.ex ciro.and in animal models.Results from the toxicity studies in animal models suggest safety profile of AL,and its active constituents.Despite extensive use with positive impression in many diseases,there has not been a clinical study that can conclusively support its efficacy and safely profile in human.This review comprehensively summarizes current information on the pharmacological activities of AL and their active constituents including anticancer,anti-inflammatory,antimicrobial and antipyretic activities,as well as activities on central nervous,cardiovascular,and gastrointestinal systems.展开更多
The present study was designed to explore the influence of water extracts of Atractylodes lancea rhizomes on the toxicity and anti-inflammatory effects of triptolide(TP). A water extract was prepared from A. lancea rh...The present study was designed to explore the influence of water extracts of Atractylodes lancea rhizomes on the toxicity and anti-inflammatory effects of triptolide(TP). A water extract was prepared from A. lancea rhizomes and co-administered with TP in C57BL/6 mice. The toxicity was assayed by determining serum biochemical parameters and visceral indexes and by liver histopathological analysis. The hepatic CYP3A expression levels were detected using Western blotting and RT-PCR methods. The data showed that the water extract of A. lancea rhizomes reduced triptolide-induced toxicity, probably by inducing the hepatic expression of CYP3A. The anti-inflammatory effects of TP were evaluated in mice using a xylene-induced ear edema test. By comparing ear edema inhibition rates, we found that the water extract could also increase the anti-inflammatory effects of TP. In conclusion, our results suggested that the water extract of A. lancea rhizomes, used in combination with TP, has a potential in reducing TP-induced toxicity and enhancing its anti-inflammatory effects.展开更多
A new polyacetylene glycoside,（1,3Z,11E）-tridecatriene-7,9-diyne-5-hydroxyl-6-O-β-D-glucopyranoside,was isolated from the rhizomes of Atractylodes lancea（Thunb.） DC.Its structure was elucidated on the basis of sp...A new polyacetylene glycoside,（1,3Z,11E）-tridecatriene-7,9-diyne-5-hydroxyl-6-O-β-D-glucopyranoside,was isolated from the rhizomes of Atractylodes lancea（Thunb.） DC.Its structure was elucidated on the basis of spectral analysis.展开更多
A new 7-9'-monoepoxy tetrahydrofuran type lignan glucoside with the cis-relationship of H-7 and H-8, named tibeticoside A 1, was isolated from the medicinal whole plants of Lancea tibetica. Its structure was eluci...A new 7-9'-monoepoxy tetrahydrofuran type lignan glucoside with the cis-relationship of H-7 and H-8, named tibeticoside A 1, was isolated from the medicinal whole plants of Lancea tibetica. Its structure was elucidated by spectroscopic methods and chemical transformation.展开更多
Four new glycosides including a eudesmane-type sesquiterpenoid（1）, a guaiane-type sesquiterpenoid（2）, and two C_（14）-polyacetylenes（3, 4） were isolated from the rhizomes of Atractylodes lancea. Their structure...Four new glycosides including a eudesmane-type sesquiterpenoid（1）, a guaiane-type sesquiterpenoid（2）, and two C_（14）-polyacetylenes（3, 4） were isolated from the rhizomes of Atractylodes lancea. Their structures were elucidated by means of spectroscopic and spectrometric analyses（UV, IR, 1D and 2D NMR, and HR-ESIMS）. The absolute configurations of their aglycones were established based on the experimental and calculated electronic circular dichroism（ECD）, whereas those of monosaccharide moieties were determined by the GC method after chiral derivatization. Compound 4 showed weak anti-inflammatory effects on the LPS-induced NO production in microglia BV2 cells at a concentration of10μmmolL^（-1）.展开更多
Cholangiocarcinoma(CCA), the adenocarcinoma of the biliary duct, is commonly reported in Asia with the highest incidence in northeastern Thailand. Chemotherapy of this type of cancer is limited due to the lack of effe...Cholangiocarcinoma(CCA), the adenocarcinoma of the biliary duct, is commonly reported in Asia with the highest incidence in northeastern Thailand. Chemotherapy of this type of cancer is limited due to the lack of effective chemotherapeutic drugs. A series of previous studies support further research and development of Atractylodes lancea(Thunb) DC.(AL) as a potential candidate for the treatment of CCA as a crude ethanolic extract. In the present study, we aimed to develop an oral pharmaceutical formulation(capsule) of the standardized AL crude ethanolic extract for further clinical development in patients with CCA. Major steps included macroscopic and microscopic authentication of the AL rhizomes, preparation of standardized AL extract, preparation of oral pharmaceutical formulation(capsule) of the standardized AL extract, quantitative and qualitative analysis of the marker compound(atractylodin) in the formulated AL extract, evaluation of contaminations of heavy metals, pesticides residues, and microorganisms in the ground AL rhizomes and the formulated(capsule) powder of AL, physicochemical and pharmaceutical properties of the formulated AL extract/capsule, and cytotoxicity evaluation of the formulated AL extract. Results of all evaluations confirmed satisfactory pharmaceutical properties of oral(capsule) formulation of the standardized AL extract.展开更多
Atractylodes lancea(Thunb.)DC.(AL)has been proven to be effective in the treatment of coronavirus disease2019(COVID-19).In this study,TCMSP,TCMID,OMIM,GeneCards,PharmMapper and SwissTargetPrediction were used to colle...Atractylodes lancea(Thunb.)DC.(AL)has been proven to be effective in the treatment of coronavirus disease2019(COVID-19).In this study,TCMSP,TCMID,OMIM,GeneCards,PharmMapper and SwissTargetPrediction were used to collect potential targets for AL against COVID-19.The online STRING analysis platform and Cytoscape were used for generating a(protein-protein interaction)PPI network.The Cytoscape and Autodock software were used for determining hub genes and key compounds.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed via DAVID database.A total of 84 common targets were obtained.The antiviral pathways were main pathways in traetment.10 hub genes and key compounds were screened by CytoHubba.We found that AL2,AL6 and AL38 had lower binding energy with key proteins.Our study demonstrated that AL might be used to treat COVID-19 by improving the"cytokine storm",regulating some antiviral pathways,and inhibiting the key protein through which the SARS-CoV-2 invades the host cell.These findings give a pharmacological basis and support for treating COVID-19 with AL.展开更多
[Objectives]To predict the mechanism of Atractylodes lancea in the treatment of diabetes by using the method of network pharmacology.[Methods]The active constituents of A.lancea were mined and their action targets wer...[Objectives]To predict the mechanism of Atractylodes lancea in the treatment of diabetes by using the method of network pharmacology.[Methods]The active constituents of A.lancea were mined and their action targets were predicted by using TCMSP database,and the targets of diabetes were screened out by GeneCards,and OMIM databases.The"constituent-target-pathway-disease"network of A.lancea in the treatment of diabetes was constructed by using Cytoscape software.The target protein-related interaction network was constructed by STRING database,and the biological function annotation and pathway analysis of key targets were carried out.[Results]The 4 active constituents of A.lancea could treat diabetes by intervening in 47 targets,95 GO biological processes and 116 main biological pathways.[Conclusions]The wogonin,NSC63551,3β-acetoxy atractylone andβ-carotene in A.lancea may regulate nuclear receptor activity,transcription factor activity(directly bind to the ligand-regulated sequence-specific DNA),steroid receptor,G protein-coupled amine receptor activity,sodium-chloride transporter activity,neurotransmitter receptor activity and other pharmacological effects,by mediating AGE-RAGE pathway,IL-17 pathway,PI3K-Akt pathway,p53 pathway,etc.,so as to treat diabetes.展开更多
Due to over exploitation, the Atractylodes resources in such five counties and cities as Jurong, Jintan, Lishui, Liyang and Gaochun have been exhausted. This paper summarized the seed propagation technique of Atractyl...Due to over exploitation, the Atractylodes resources in such five counties and cities as Jurong, Jintan, Lishui, Liyang and Gaochun have been exhausted. This paper summarized the seed propagation technique of Atractylodes lancea in Maoshan, Jiangsu Province, aiming to provide reference for the restoration of A. lancea production in Maoshan, Jiangsu.展开更多
According to the current situation of diseases on Atractylodes lancea , the etiology, pathogenesis, symptoms and control methods of root diseases, leaf diseases and other diseases of A. lancea are analyzed, in order t...According to the current situation of diseases on Atractylodes lancea , the etiology, pathogenesis, symptoms and control methods of root diseases, leaf diseases and other diseases of A. lancea are analyzed, in order to provide a reference for the diagnosis and control of diseases on A. lancea .展开更多
Atractylodes lancea(called Cangzhu in China)is a medicinal plant that has long been used as tonic agent in various ethno-medical systems in East Asia,especially in China,for the treatment of gastrointestinal dysfuncti...Atractylodes lancea(called Cangzhu in China)is a medicinal plant that has long been used as tonic agent in various ethno-medical systems in East Asia,especially in China,for the treatment of gastrointestinal dysfunction,cancer,osteoporosis,obesity and fetal irritability.We used the TCMSP database to search for the main active ingredients and traditional Chinese medicine targets of Atractylodes macrocephala.There are a total of 38 related articles,of which 27 are closely related to chemical composition and activity.This study reviews the chemical components and pharmacological effects of A.lancea,aiming to provide reference for its further research and development.展开更多
Objective:The objective of the study was to evaluate the clinical efficacy,safety,immunomodulatory activity,and effects on quality of life(QoL)of a capsule formulation of Atractylodes lancea(Thunb.)DC.Chemistry Manufa...Objective:The objective of the study was to evaluate the clinical efficacy,safety,immunomodulatory activity,and effects on quality of life(QoL)of a capsule formulation of Atractylodes lancea(Thunb.)DC.Chemistry Manufacturing and Control capsule formulation of standardized extract of A.lancea(CMC-AL)in patients with advanced stage of intrahepatic cholangiocarcinoma(iCCA).Patients and Methods:A total of eligible 48 patients were randomly assigned to one of three groups.Group 1 received a once-daily dose of 1000 mg of CMC-AL in combination with palliative care for 3 months(n=16).Group 2 received escalating doses of 1000,1500,and 2000 mg of CMC-AL,along with palliative care for 3 months(n=16).The control group received palliative care alone(n=16).All patients were followed up for 4 months.Serum cytokine levels were measured using a bead array,and safety and treatment responses were evaluated according to standard criteria.Results:CMC-AL at both dosage levels was well tolerated.The high-dose regimen(Group 2)significantly improved the patients'clinical responses and QoL.Furthermore,there was a notable improvement in median total QoL scores compared to the baseline.Serum interleukin-6 levels on day 14 in both Groups 1 and 2 significantly decreased compared to baseline measurements.Conclusions:Results showed a promising role for CMC-AL in the treatment and management of iCCA.The high-dose CMC-AL showed advantages over the low-dose formulation and palliative care alone in enhancing clinical response,minimizing disease progression,improving QoL,and exhibiting immune-stimulatory effects.Therefore,the high-dose regimen could serve as an alternative treatment for iCCA patients who decline chemotherapy.展开更多
Fungal endophytes have been isolated from almost every plant, infecting their hosts without causing visible disease symptoms, and yet have still proved to be involved in plant secondary metabolites accumulation. To de...Fungal endophytes have been isolated from almost every plant, infecting their hosts without causing visible disease symptoms, and yet have still proved to be involved in plant secondary metabolites accumulation. To decipher the possible physiological mechanisms of the endophytic fungus-host interaction, the role of protein phosphorylation and the relationship between endophytic fungus-induced kinase activity and nitric oxide （NO） and brassinolide （BL） in endophyte-enhanced volatile oil accumulation in Atractylodes lancea plantlets were investigated using pharmacological and biochemical approaches. Inoculation with the endophytic fungus Gilmaniella sp. ALl2 enhanced the activities of total protein phosphorylation, Ca2^-dependent protein kinase, and volatile oil accumulation in A. lancea plantlets. The upregulation of protein kinase activity could be blocked by the BL inhibitor brassinazole. Furthermore, pretreatments with the NO-specific scavenger cPTIO significantly reduced the increased activities of protein kinases in A. lancea plantlets inoculated with endophytic fungus. Pretreatments with different protein kinase inhibitors also reduced fungus-induced NO production and volatile oil accumulation, but had barely no effect on the BL level. These data suggest that protein phosphorylation is required for endophyte- induced volatile oil production in A. lancea plantlets, and that crosstalk between protein phosphorylation and the NO pathway may occur and act as a downstream signaling event of the BL pathway.展开更多
Objective To study the water-soluble chemical constituents from the rhizomes of Atratylodes lancea. Methods Two sesquiterpenoid glycosides were purified by column chromatography and their structures were determined by...Objective To study the water-soluble chemical constituents from the rhizomes of Atratylodes lancea. Methods Two sesquiterpenoid glycosides were purified by column chromatography and their structures were determined by spectroscopic analysis. They were also evaluated for anti-inflammatory activity by determining the inhibitory activity on LPS-induced NO and PGE2 generation in RAW 264.7 cell lines. Results Compound 1 was a new sesquiterpenoid glycoside, named as（1S,4S,5R,7R,10S）-4,11,14-trihydroxyguai-3-one-11-O-β-D-glucopyranoside, but exhibited no appreciable activity. Compound 2 was atractyloside A and showed weak activity. Conclusion The hydroxyl group at C-10 of guaiol-type glycoside could be important for anti-inflammatory activity.展开更多
基金Supported by the National Natural Science Foundation of China(31070443)the National Science Foundation of China for Talent Training in Basic Research(J1103507)+1 种基金The Priority Academic Program Development of Jiangsu Higher Education Institutions,Innovation Promotion Project of Nanjing Municipal Science and Technology Commissions(201105058)the Practice and Innovation Training Program for the Students of Nanjing Normal University~~
文摘[Objective] This study was to explore the effect of three precursors on the accumulation of principal volatile oil constituents in tissue culture plantlets of Atractylodes lancea （Thunb.） DC. with the aim to provide references for the improvement of artificial cultivated A. lancea quality. [Method] Three precursors were added into the MS rooting medium for A. lancea tissue culture plantlets and the volatile oil was extracted by ultrasonication after cultured for several days. The content of atractylon, atractylol, β-eucalyptol and atractydin in the volatile oil were determined by using gas chromatography method. [Result] The addition of xylose, isoprene and tetrahydrofuran impacted the growth indicators, yield of volatile oil and relative percentage content of the four constituents of A. lancea tissue culture plantlets. In the 6 g/L xylose optimized medium, the atractylon and β-eucalyptol content reached up to 4.23% and 56.34%, respectively, 1.41% and 1.66% higher than the control; although the addition of isoprene into medium raised the atractylon content, the accumulation of total volatile oil was inhibited that it decreased by 23.67%, 31.06% and 7.10% to the control; for the tetrahydrofuran optimized medium, the content of atractylon, atractylol and atractydin all increased, and the total volatile oil content increased by 49.97% to the control when the concentration of tetrahydrofuran was 0.07 g/L. [Conclusion] The addition of xylose and tetrahydrofuran promoted the accumulation of principal constituents of the volatile oil, whereas the addition of isoprene inhibited the accumulation.
基金Supported by the National Natural Science Foundation of China(31070443,30970523)the National Science Foundation for Talents Training in Basic Science,China(J1103507)the Project of the Priority Academic Program Development of Jiangsu Higher Education Institutions,China~~
文摘[Objective] To examine the effect of endophytic fungi ALl2 （Gilmaniella sp.） on metabolites distribution in organs of Atractylodes lancea. [Method] Endophytic fungi ALl2 was inoculated on Atraetylodes lancea plantlets in tissue culture, and the distribution of cellulose, hemicellulose, lignin, soluble sugar in leaves and roots of the inoculated group were detected. The weight of leaves and roots were compared. Gas Chromatography was used to analyze the volatile oil components. [Result] Compared with the control group, the average fresh weight and dry weight of leaves and roots of A. lancea which had been symbiosed with ALl2 increased significantly. The content of lignin and soluble sugar increased in the leaves of the inoculated group, and the content of cellulose, hemicellulose, lignin, soluble sugar and volatile oil also increased in roots. [Conclusion] The results indicate that symbiosed with ALl2 is benefit for the development of A. lancea roots and can promote the transfer and accumulation of the medicinal components to the roots.
文摘The rhizome of Atractylodes Iancen(A.lancea)(Thunb.) DC.(AL)is extensively used in Chinese,Thai,and Japanese traditional medicines as crude extracts/decoctions or a component in various herbal formulations.Various pharmacological activities of Al.and its major constituents have been demonstrated in ritro.ex ciro.and in animal models.Results from the toxicity studies in animal models suggest safety profile of AL,and its active constituents.Despite extensive use with positive impression in many diseases,there has not been a clinical study that can conclusively support its efficacy and safely profile in human.This review comprehensively summarizes current information on the pharmacological activities of AL and their active constituents including anticancer,anti-inflammatory,antimicrobial and antipyretic activities,as well as activities on central nervous,cardiovascular,and gastrointestinal systems.
基金supported by the National Natural Science Foundation of China(Nos.81102522 and 81373480)the Natural Science Foundation of Jiangsu Province(No.BK2011473)the Excellent Youth Foundation of Jiangsu University
文摘The present study was designed to explore the influence of water extracts of Atractylodes lancea rhizomes on the toxicity and anti-inflammatory effects of triptolide(TP). A water extract was prepared from A. lancea rhizomes and co-administered with TP in C57BL/6 mice. The toxicity was assayed by determining serum biochemical parameters and visceral indexes and by liver histopathological analysis. The hepatic CYP3A expression levels were detected using Western blotting and RT-PCR methods. The data showed that the water extract of A. lancea rhizomes reduced triptolide-induced toxicity, probably by inducing the hepatic expression of CYP3A. The anti-inflammatory effects of TP were evaluated in mice using a xylene-induced ear edema test. By comparing ear edema inhibition rates, we found that the water extract could also increase the anti-inflammatory effects of TP. In conclusion, our results suggested that the water extract of A. lancea rhizomes, used in combination with TP, has a potential in reducing TP-induced toxicity and enhancing its anti-inflammatory effects.
文摘A new polyacetylene glycoside,（1,3Z,11E）-tridecatriene-7,9-diyne-5-hydroxyl-6-O-β-D-glucopyranoside,was isolated from the rhizomes of Atractylodes lancea（Thunb.） DC.Its structure was elucidated on the basis of spectral analysis.
文摘A new 7-9'-monoepoxy tetrahydrofuran type lignan glucoside with the cis-relationship of H-7 and H-8, named tibeticoside A 1, was isolated from the medicinal whole plants of Lancea tibetica. Its structure was elucidated by spectroscopic methods and chemical transformation.
基金funded by the National Science and Technology Project of China(No.2012ZX09301002-001003)
文摘Four new glycosides including a eudesmane-type sesquiterpenoid（1）, a guaiane-type sesquiterpenoid（2）, and two C_（14）-polyacetylenes（3, 4） were isolated from the rhizomes of Atractylodes lancea. Their structures were elucidated by means of spectroscopic and spectrometric analyses（UV, IR, 1D and 2D NMR, and HR-ESIMS）. The absolute configurations of their aglycones were established based on the experimental and calculated electronic circular dichroism（ECD）, whereas those of monosaccharide moieties were determined by the GC method after chiral derivatization. Compound 4 showed weak anti-inflammatory effects on the LPS-induced NO production in microglia BV2 cells at a concentration of10μmmolL^（-1）.
基金Financial support by National Research Council of Thailand(NRCT)Center of Excellence in Pharmacology and Molecular Biology of Malaria+1 种基金Cholangiocarcinoma and Chulabhorn International College of Medicine(CICM)Thammasat University,Thailand
文摘Cholangiocarcinoma(CCA), the adenocarcinoma of the biliary duct, is commonly reported in Asia with the highest incidence in northeastern Thailand. Chemotherapy of this type of cancer is limited due to the lack of effective chemotherapeutic drugs. A series of previous studies support further research and development of Atractylodes lancea(Thunb) DC.(AL) as a potential candidate for the treatment of CCA as a crude ethanolic extract. In the present study, we aimed to develop an oral pharmaceutical formulation(capsule) of the standardized AL crude ethanolic extract for further clinical development in patients with CCA. Major steps included macroscopic and microscopic authentication of the AL rhizomes, preparation of standardized AL extract, preparation of oral pharmaceutical formulation(capsule) of the standardized AL extract, quantitative and qualitative analysis of the marker compound(atractylodin) in the formulated AL extract, evaluation of contaminations of heavy metals, pesticides residues, and microorganisms in the ground AL rhizomes and the formulated(capsule) powder of AL, physicochemical and pharmaceutical properties of the formulated AL extract/capsule, and cytotoxicity evaluation of the formulated AL extract. Results of all evaluations confirmed satisfactory pharmaceutical properties of oral(capsule) formulation of the standardized AL extract.
文摘Atractylodes lancea(Thunb.)DC.(AL)has been proven to be effective in the treatment of coronavirus disease2019(COVID-19).In this study,TCMSP,TCMID,OMIM,GeneCards,PharmMapper and SwissTargetPrediction were used to collect potential targets for AL against COVID-19.The online STRING analysis platform and Cytoscape were used for generating a(protein-protein interaction)PPI network.The Cytoscape and Autodock software were used for determining hub genes and key compounds.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed via DAVID database.A total of 84 common targets were obtained.The antiviral pathways were main pathways in traetment.10 hub genes and key compounds were screened by CytoHubba.We found that AL2,AL6 and AL38 had lower binding energy with key proteins.Our study demonstrated that AL might be used to treat COVID-19 by improving the"cytokine storm",regulating some antiviral pathways,and inhibiting the key protein through which the SARS-CoV-2 invades the host cell.These findings give a pharmacological basis and support for treating COVID-19 with AL.
基金Guangdong Provincial Administration of Traditional Chinese Medicine(20182170)Special Project for Zhongshan Social Welfare Science and Technology Research(2018B1010)。
文摘[Objectives]To predict the mechanism of Atractylodes lancea in the treatment of diabetes by using the method of network pharmacology.[Methods]The active constituents of A.lancea were mined and their action targets were predicted by using TCMSP database,and the targets of diabetes were screened out by GeneCards,and OMIM databases.The"constituent-target-pathway-disease"network of A.lancea in the treatment of diabetes was constructed by using Cytoscape software.The target protein-related interaction network was constructed by STRING database,and the biological function annotation and pathway analysis of key targets were carried out.[Results]The 4 active constituents of A.lancea could treat diabetes by intervening in 47 targets,95 GO biological processes and 116 main biological pathways.[Conclusions]The wogonin,NSC63551,3β-acetoxy atractylone andβ-carotene in A.lancea may regulate nuclear receptor activity,transcription factor activity(directly bind to the ligand-regulated sequence-specific DNA),steroid receptor,G protein-coupled amine receptor activity,sodium-chloride transporter activity,neurotransmitter receptor activity and other pharmacological effects,by mediating AGE-RAGE pathway,IL-17 pathway,PI3K-Akt pathway,p53 pathway,etc.,so as to treat diabetes.
文摘Due to over exploitation, the Atractylodes resources in such five counties and cities as Jurong, Jintan, Lishui, Liyang and Gaochun have been exhausted. This paper summarized the seed propagation technique of Atractylodes lancea in Maoshan, Jiangsu Province, aiming to provide reference for the restoration of A. lancea production in Maoshan, Jiangsu.
基金Supported by Key Research and Development Project of Hubei Province (2020BBB091)Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization (2021ZD09)+1 种基金Doctoral Research Foundation of Huanggang Normal University (2042020014)Innovation and Entrepreneurship Training Program for College Students (202110514157).
文摘According to the current situation of diseases on Atractylodes lancea , the etiology, pathogenesis, symptoms and control methods of root diseases, leaf diseases and other diseases of A. lancea are analyzed, in order to provide a reference for the diagnosis and control of diseases on A. lancea .
基金This work was financially supported by National Nature Science Foundation of China(81973284)Scientific Research Foundation of the Education Department of Liaoning Province(LJKZ0944).
文摘Atractylodes lancea(called Cangzhu in China)is a medicinal plant that has long been used as tonic agent in various ethno-medical systems in East Asia,especially in China,for the treatment of gastrointestinal dysfunction,cancer,osteoporosis,obesity and fetal irritability.We used the TCMSP database to search for the main active ingredients and traditional Chinese medicine targets of Atractylodes macrocephala.There are a total of 38 related articles,of which 27 are closely related to chemical composition and activity.This study reviews the chemical components and pharmacological effects of A.lancea,aiming to provide reference for its further research and development.
基金funded by the Thailand Science Research and Innovation Fundamental Fund for the Fiscal Year 2024supported by Thammasat University Research Fund,Contract No.TUFT-FF 48/2565funded by the National Research Council of Thailand(NRCT),Contract number N42A671041。
文摘Objective:The objective of the study was to evaluate the clinical efficacy,safety,immunomodulatory activity,and effects on quality of life(QoL)of a capsule formulation of Atractylodes lancea(Thunb.)DC.Chemistry Manufacturing and Control capsule formulation of standardized extract of A.lancea(CMC-AL)in patients with advanced stage of intrahepatic cholangiocarcinoma(iCCA).Patients and Methods:A total of eligible 48 patients were randomly assigned to one of three groups.Group 1 received a once-daily dose of 1000 mg of CMC-AL in combination with palliative care for 3 months(n=16).Group 2 received escalating doses of 1000,1500,and 2000 mg of CMC-AL,along with palliative care for 3 months(n=16).The control group received palliative care alone(n=16).All patients were followed up for 4 months.Serum cytokine levels were measured using a bead array,and safety and treatment responses were evaluated according to standard criteria.Results:CMC-AL at both dosage levels was well tolerated.The high-dose regimen(Group 2)significantly improved the patients'clinical responses and QoL.Furthermore,there was a notable improvement in median total QoL scores compared to the baseline.Serum interleukin-6 levels on day 14 in both Groups 1 and 2 significantly decreased compared to baseline measurements.Conclusions:Results showed a promising role for CMC-AL in the treatment and management of iCCA.The high-dose CMC-AL showed advantages over the low-dose formulation and palliative care alone in enhancing clinical response,minimizing disease progression,improving QoL,and exhibiting immune-stimulatory effects.Therefore,the high-dose regimen could serve as an alternative treatment for iCCA patients who decline chemotherapy.
基金the National Natural Science Foundation of China (No. 31070443 and No. 30970523)
文摘Fungal endophytes have been isolated from almost every plant, infecting their hosts without causing visible disease symptoms, and yet have still proved to be involved in plant secondary metabolites accumulation. To decipher the possible physiological mechanisms of the endophytic fungus-host interaction, the role of protein phosphorylation and the relationship between endophytic fungus-induced kinase activity and nitric oxide （NO） and brassinolide （BL） in endophyte-enhanced volatile oil accumulation in Atractylodes lancea plantlets were investigated using pharmacological and biochemical approaches. Inoculation with the endophytic fungus Gilmaniella sp. ALl2 enhanced the activities of total protein phosphorylation, Ca2^-dependent protein kinase, and volatile oil accumulation in A. lancea plantlets. The upregulation of protein kinase activity could be blocked by the BL inhibitor brassinazole. Furthermore, pretreatments with the NO-specific scavenger cPTIO significantly reduced the increased activities of protein kinases in A. lancea plantlets inoculated with endophytic fungus. Pretreatments with different protein kinase inhibitors also reduced fungus-induced NO production and volatile oil accumulation, but had barely no effect on the BL level. These data suggest that protein phosphorylation is required for endophyte- induced volatile oil production in A. lancea plantlets, and that crosstalk between protein phosphorylation and the NO pathway may occur and act as a downstream signaling event of the BL pathway.
基金Open Fund of Jiangsu Key Laboratory for Bioresources of Saline Solis(JKLB2013005)NSFC(31401898,31470425,and 31570359)
文摘Objective To study the water-soluble chemical constituents from the rhizomes of Atratylodes lancea. Methods Two sesquiterpenoid glycosides were purified by column chromatography and their structures were determined by spectroscopic analysis. They were also evaluated for anti-inflammatory activity by determining the inhibitory activity on LPS-induced NO and PGE2 generation in RAW 264.7 cell lines. Results Compound 1 was a new sesquiterpenoid glycoside, named as（1S,4S,5R,7R,10S）-4,11,14-trihydroxyguai-3-one-11-O-β-D-glucopyranoside, but exhibited no appreciable activity. Compound 2 was atractyloside A and showed weak activity. Conclusion The hydroxyl group at C-10 of guaiol-type glycoside could be important for anti-inflammatory activity.
