Objective:The Hutchinson-Gilford progeria syndrome (HGPS or progeria) is a childhood disorder with features of premature aging and is caused by mutations in the lamin A gene resulting in the production of an abnormal ...Objective:The Hutchinson-Gilford progeria syndrome (HGPS or progeria) is a childhood disorder with features of premature aging and is caused by mutations in the lamin A gene resulting in the production of an abnormal protein,termed progerin. To investigate the underlying pathogenic mechanism,we studied the nuclear co-localization and association of progerin interactive partner proteins (PIPPs) with lamina proteins. Methods:Both wild-type (WT) and progeria fibroblasts were studied by various methods including confocal microscopy,immunoprecipitation and Western blot.Results:All PIPPs discovered so-far co-localized with lamin A/C. In addition,the PIPPs were selectively associated with lamina proteins. An increased immunofluorescent staining signal was found for Mel18 in HGPS as compared to WT cells. An association of Mel18 with emerin was observed in HGPS,but not in WT cells. Conclusion:Based on these findings,we propose that PIPPs,along with associated lamina proteins may form a pathogenic progerin-containing protein complex.展开更多
In this study,an in-depth analysis of the types,characteristics,and formation mechanisms of microlaminae and microscopic laminae was conducted in order to precisely examine the link or intersection of stratigraphy and...In this study,an in-depth analysis of the types,characteristics,and formation mechanisms of microlaminae and microscopic laminae was conducted in order to precisely examine the link or intersection of stratigraphy and petrology.This study was essentially a sedimentary examination of the minuteness-macro and micro-tiny layers between laminae and pore structure,as well as the types of structures and sedimentation.The results of this study bear important basic subject attributes and significance,as well as practical value for the basic theories and exploration applications of unconventional oil and gas geology.The quantitative data were obtained using the following:field macroscopic observations;measurements;intensive sampling processes;XRD mineral content analysis;scanning electron microscopy;high-power polarizing microscope observations;and micro-scale measurements.The quantitative parameters,such as laminae thicknesses,laminae properties,organic matter laminae,and laminae spatial distributions were unified within a framework,and the correlations among them were established for the purpose of forming a fine-grained deposition micro-laminae evaluation system.The results obtained in this research investigation established a basis for the classification of micro-laminae,and divided the micro-laminae into four categories and 20 sub-categories according to the development thicknesses,material compositions,organic matter content levels,and the spatial distributions of the micro-laminae.The classification scheme of the micro-laminae was divided into two categories and 12 sub-categories.Then,in accordance with the comprehensive characteristics of spatial morphology,the micro-laminae was further divided into the following categories:continuous horizontal laminae;near horizontal laminae;slow wavy laminae;wavy laminae;discontinuous laminae;and lenticular laminae.According to the structural properties of the laminae development,the micro-laminae was divided into the following categories:single laminae structures;laminated laminae structures;interlaminar structures;multiple mixed laminae structures;cyclic laminae structures;and progressive laminae structures.The research results were considered to be applicable for the scientific evaluations of reservoir spaces related to unconventional oil and gas resources.展开更多
文摘Objective:The Hutchinson-Gilford progeria syndrome (HGPS or progeria) is a childhood disorder with features of premature aging and is caused by mutations in the lamin A gene resulting in the production of an abnormal protein,termed progerin. To investigate the underlying pathogenic mechanism,we studied the nuclear co-localization and association of progerin interactive partner proteins (PIPPs) with lamina proteins. Methods:Both wild-type (WT) and progeria fibroblasts were studied by various methods including confocal microscopy,immunoprecipitation and Western blot.Results:All PIPPs discovered so-far co-localized with lamin A/C. In addition,the PIPPs were selectively associated with lamina proteins. An increased immunofluorescent staining signal was found for Mel18 in HGPS as compared to WT cells. An association of Mel18 with emerin was observed in HGPS,but not in WT cells. Conclusion:Based on these findings,we propose that PIPPs,along with associated lamina proteins may form a pathogenic progerin-containing protein complex.
文摘In this study,an in-depth analysis of the types,characteristics,and formation mechanisms of microlaminae and microscopic laminae was conducted in order to precisely examine the link or intersection of stratigraphy and petrology.This study was essentially a sedimentary examination of the minuteness-macro and micro-tiny layers between laminae and pore structure,as well as the types of structures and sedimentation.The results of this study bear important basic subject attributes and significance,as well as practical value for the basic theories and exploration applications of unconventional oil and gas geology.The quantitative data were obtained using the following:field macroscopic observations;measurements;intensive sampling processes;XRD mineral content analysis;scanning electron microscopy;high-power polarizing microscope observations;and micro-scale measurements.The quantitative parameters,such as laminae thicknesses,laminae properties,organic matter laminae,and laminae spatial distributions were unified within a framework,and the correlations among them were established for the purpose of forming a fine-grained deposition micro-laminae evaluation system.The results obtained in this research investigation established a basis for the classification of micro-laminae,and divided the micro-laminae into four categories and 20 sub-categories according to the development thicknesses,material compositions,organic matter content levels,and the spatial distributions of the micro-laminae.The classification scheme of the micro-laminae was divided into two categories and 12 sub-categories.Then,in accordance with the comprehensive characteristics of spatial morphology,the micro-laminae was further divided into the following categories:continuous horizontal laminae;near horizontal laminae;slow wavy laminae;wavy laminae;discontinuous laminae;and lenticular laminae.According to the structural properties of the laminae development,the micro-laminae was divided into the following categories:single laminae structures;laminated laminae structures;interlaminar structures;multiple mixed laminae structures;cyclic laminae structures;and progressive laminae structures.The research results were considered to be applicable for the scientific evaluations of reservoir spaces related to unconventional oil and gas resources.
