A plasmid (pTU9) containing the lambda (λ) phage lysis genes S( )RRz and the biosynthetic genes phb CAB of poly β hydroxybutyrate (PHB) was constructed and transformed into E.coli JM109. Cultured in Luria Ber...A plasmid (pTU9) containing the lambda (λ) phage lysis genes S( )RRz and the biosynthetic genes phb CAB of poly β hydroxybutyrate (PHB) was constructed and transformed into E.coli JM109. Cultured in Luria Bertani (LB) medium with 20 g/L glucose, E.coli JM109 (pTU9) could accumulate PHB in cells up to 40% (g PHB per g dry cells). A chelating agent EDTA was applied to induce a complete cell lysis and PHB granules were released. This method has a potential application in PHB separation.展开更多
文摘A plasmid (pTU9) containing the lambda (λ) phage lysis genes S( )RRz and the biosynthetic genes phb CAB of poly β hydroxybutyrate (PHB) was constructed and transformed into E.coli JM109. Cultured in Luria Bertani (LB) medium with 20 g/L glucose, E.coli JM109 (pTU9) could accumulate PHB in cells up to 40% (g PHB per g dry cells). A chelating agent EDTA was applied to induce a complete cell lysis and PHB granules were released. This method has a potential application in PHB separation.
