Background Perilla frutescens seeds(PFS)are gaining recognition as a natural alternative to antibiotics in livestock,supporting sustainable farming and animal health.However,the underlying molecular mechanisms through...Background Perilla frutescens seeds(PFS)are gaining recognition as a natural alternative to antibiotics in livestock,supporting sustainable farming and animal health.However,the underlying molecular mechanisms through which PFS influence host immune function and antioxidant capacity,especially via the gut-liver-muscle axis,remain largely unknown.This study employed an integrative multi-omics approach to elucidate how PFS supplementation modulates the microbiota-gut-liver-muscle axis and enhances immune and antioxidant functions in lambs.Results PFS supplementation markedly improved immune and antioxidant profiles,demonstrated by elevated serum levels of IL-10,IgM,IgG,GSH-PX,and SOD,and reductions in IL-1β,TNF-α,and MDA.Microbial analysis revealed elevated abundances of ruminal and intestinal taxa commonly associated with gut homeostasis and metabolic health(Christensenellaceae_R-7_group)and reduced levels of species with pathogenic or pro-inflammatory potential(Bacillus cereus and Clostridioides)in the ileum.Transcriptomic and metabolomic profiling of liver tissue indicated modulation of key inflammatory and bile acid signaling pathways,including the downregulation of TLR4,NLRP3,ATF3,CYP2J2,and LXR-α.PFS also increased hepatic concentrations of anti-inflammatory metabolites such as chlorquinaldol and indole-3-carboxaldehyde,while reducing levels of LysoPC(20:4)and phosphatidic acid.Correlation and mediation analyses revealed strong interconnections among gut microbiota,hepatic gene expression,lipid metabolites in liver and muscle,and systemic immune-antioxidant markers.Conclusion These findings highlight the microbiota-gut-liver-muscle axis as a central mechanism through which PFS enhances immune function and antioxidant capacity in lambs.PFS supplementation represents a promising nutritional strategy to improve healthy lamb production,supporting the development of antibiotic-free and sustainable livestock systems.展开更多
Lamb波具有较强的抗干扰能力,被广泛应用于碳纤维增强树脂(Carbon Fiber Reinforced Polymer,CFRP)结构健康监测中。采用汉宁窗形式的窄带Lamb波激励完整CFRP板与含内置损伤的损伤CFRP板,通过比较PZT压电片采集到的健康信号与损伤信号...Lamb波具有较强的抗干扰能力,被广泛应用于碳纤维增强树脂(Carbon Fiber Reinforced Polymer,CFRP)结构健康监测中。采用汉宁窗形式的窄带Lamb波激励完整CFRP板与含内置损伤的损伤CFRP板,通过比较PZT压电片采集到的健康信号与损伤信号之间的信号差异系数,根据改进的损伤概率重建算法(Reconstruction Algorithm for the Probabilistic Inspection of Damage,RAPID)实现CFRP结构的内部损伤成像。得到以下结论:建立CFRP板Lamb波有限元模型对内置损伤的大小和区域进行定位,并根据CFRP板Lamb波结构健康监测试验平台验证有限元模型的正确性;提出一种阈值化改进RAPID算法,对损伤板的内置损伤大小和轮廓进行准确预测,预测结果最大误差仅为6.56%;随着结构内置损伤孔厚度的增加,结构损伤信号与健康信号的差异越发明显,预测损伤参数与实际损伤参数的最小误差仅为5.20 mm。展开更多
基金supported by the Central Government-Guided Local Science and Technology Development Fund Project(2025ZY0108)Bayannaoer Research Institute Young Scientist Project(2024BYNECAU002)+1 种基金Hainan Provincial Natural Science Foundation of China(324QN289)the China Agriculture Research System(CARS-38)。
文摘Background Perilla frutescens seeds(PFS)are gaining recognition as a natural alternative to antibiotics in livestock,supporting sustainable farming and animal health.However,the underlying molecular mechanisms through which PFS influence host immune function and antioxidant capacity,especially via the gut-liver-muscle axis,remain largely unknown.This study employed an integrative multi-omics approach to elucidate how PFS supplementation modulates the microbiota-gut-liver-muscle axis and enhances immune and antioxidant functions in lambs.Results PFS supplementation markedly improved immune and antioxidant profiles,demonstrated by elevated serum levels of IL-10,IgM,IgG,GSH-PX,and SOD,and reductions in IL-1β,TNF-α,and MDA.Microbial analysis revealed elevated abundances of ruminal and intestinal taxa commonly associated with gut homeostasis and metabolic health(Christensenellaceae_R-7_group)and reduced levels of species with pathogenic or pro-inflammatory potential(Bacillus cereus and Clostridioides)in the ileum.Transcriptomic and metabolomic profiling of liver tissue indicated modulation of key inflammatory and bile acid signaling pathways,including the downregulation of TLR4,NLRP3,ATF3,CYP2J2,and LXR-α.PFS also increased hepatic concentrations of anti-inflammatory metabolites such as chlorquinaldol and indole-3-carboxaldehyde,while reducing levels of LysoPC(20:4)and phosphatidic acid.Correlation and mediation analyses revealed strong interconnections among gut microbiota,hepatic gene expression,lipid metabolites in liver and muscle,and systemic immune-antioxidant markers.Conclusion These findings highlight the microbiota-gut-liver-muscle axis as a central mechanism through which PFS enhances immune function and antioxidant capacity in lambs.PFS supplementation represents a promising nutritional strategy to improve healthy lamb production,supporting the development of antibiotic-free and sustainable livestock systems.
文摘Lamb波具有较强的抗干扰能力,被广泛应用于碳纤维增强树脂(Carbon Fiber Reinforced Polymer,CFRP)结构健康监测中。采用汉宁窗形式的窄带Lamb波激励完整CFRP板与含内置损伤的损伤CFRP板,通过比较PZT压电片采集到的健康信号与损伤信号之间的信号差异系数,根据改进的损伤概率重建算法(Reconstruction Algorithm for the Probabilistic Inspection of Damage,RAPID)实现CFRP结构的内部损伤成像。得到以下结论:建立CFRP板Lamb波有限元模型对内置损伤的大小和区域进行定位,并根据CFRP板Lamb波结构健康监测试验平台验证有限元模型的正确性;提出一种阈值化改进RAPID算法,对损伤板的内置损伤大小和轮廓进行准确预测,预测结果最大误差仅为6.56%;随着结构内置损伤孔厚度的增加,结构损伤信号与健康信号的差异越发明显,预测损伤参数与实际损伤参数的最小误差仅为5.20 mm。
