[Objectives]This study was conducted to optimize the optimal process conditions for extracting polysaccharides from Rose laevigate Michx.with hot water.[Methods]The extraction rate of R.laevigate was used as an evalua...[Objectives]This study was conducted to optimize the optimal process conditions for extracting polysaccharides from Rose laevigate Michx.with hot water.[Methods]The extraction rate of R.laevigate was used as an evaluation index,and extraction temperature,material-to-liquid ratio,extraction time and extraction times were selected as influencing factors.On the basis of single factor tests,L 9(34)orthogonal tests were carried out for optimization,and the content of polysaccharides was determined by the phenol-sulfuric acid method.The in vitro antioxidant activity of the extracted R.laevigate polysaccharides was studied.[Results]The effects of various influencing factors on the extraction rate of R.laevigate polysaccharides ranked as material-liquid ratio>extraction temperature>extraction times>extraction time.The optimal extraction process was extraction temperature 80℃,extraction time 2 h,and material-to-liquid ratio 1∶25,extraction times 3 times,under which the extraction rate was(9.55±0.12)%.R.laevigate polysaccharides had certain scavenging capacities of DPPH free radicals and hydroxyl free radicals,and its scavenging capacity of DPPH free radicals was better than that of hydroxyl free radicals.[Conclusions]This study provides a theoretical basis for the optimization of the extraction process of R.laevigate polysaccharides and the in-depth development and application of polysaccharides.展开更多
文摘[Objectives]This study was conducted to optimize the optimal process conditions for extracting polysaccharides from Rose laevigate Michx.with hot water.[Methods]The extraction rate of R.laevigate was used as an evaluation index,and extraction temperature,material-to-liquid ratio,extraction time and extraction times were selected as influencing factors.On the basis of single factor tests,L 9(34)orthogonal tests were carried out for optimization,and the content of polysaccharides was determined by the phenol-sulfuric acid method.The in vitro antioxidant activity of the extracted R.laevigate polysaccharides was studied.[Results]The effects of various influencing factors on the extraction rate of R.laevigate polysaccharides ranked as material-liquid ratio>extraction temperature>extraction times>extraction time.The optimal extraction process was extraction temperature 80℃,extraction time 2 h,and material-to-liquid ratio 1∶25,extraction times 3 times,under which the extraction rate was(9.55±0.12)%.R.laevigate polysaccharides had certain scavenging capacities of DPPH free radicals and hydroxyl free radicals,and its scavenging capacity of DPPH free radicals was better than that of hydroxyl free radicals.[Conclusions]This study provides a theoretical basis for the optimization of the extraction process of R.laevigate polysaccharides and the in-depth development and application of polysaccharides.
