<i><span style="font-family:Verdana;">Paeonia lactiflora</span></i><span style="font-family:""><span style="font-family:Verdana;"> Pall. var. <...<i><span style="font-family:Verdana;">Paeonia lactiflora</span></i><span style="font-family:""><span style="font-family:Verdana;"> Pall. var. </span><i><span style="font-family:Verdana;">trichocarpa </span></i><span style="font-family:Verdana;">is a variety of </span><i><span style="font-family:Verdana;">Paeonia lactiflora</span></i><span style="font-family:Verdana;"> Pall., and is currently the peony herb</span></span><span style="font-family:Verdana;">’</span><span style="font-family:""><span style="font-family:Verdana;">s principal cultivar group. Here, we study the differences in aromatic components and flowers of different varieties between two groups of cultivars, providing a reference for applying natural fragrance substances of peonies, breeding fragrant flower types, and developing and </span><span style="font-family:Verdana;">using improved varieties. Headspace solid-phase microextraction (HS-SPME),</span> <span style="font-family:Verdana;">gas chromatography-mass spectrometry (GC-MS), peak area normalization for</span><span style="font-family:Verdana;"> each component relative to content, component library (NIST14/NIST14S) retrieval, and a literature review were used to analyze the volatile compounds in flowers of eight peony varieties, such as </span></span><span style="font-family:Verdana;">“</span><span style="font-family:Verdana;">Gaoganhong</span><span style="font-family:Verdana;">”</span><span style="font-family:Verdana;">, and ten comospore peony varieties, such as </span><span style="font-family:Verdana;">“</span><span style="font-family:Verdana;">Jinshanhong</span><span style="font-family:Verdana;">”</span><span style="font-family:""><span style="font-family:Verdana;">. Results showed that the main volatile compound constituents in flowers of the two groups were terpenes and alcohols. Additionally, the content of eucalyptol, caryophyllene, α-Pinene, citronellol, and 3-Hexen-1-ol, acetate, (Z) was high. Peony cultivars contained linalool,</span><span style="font-family:Verdana;"> (1R)-2,6,6-trimethylbicyclo[3.1.1]hept-2-ene, and 1,4-dimethoxybenzene,</span><span style="font-family:Verdana;"> while comospore peony varieties contained 1,3,6-octatriene, 3,7-dimethyl-, (Z)-, phenylethyl alcohol, and geraniol. In this study, the differences between the volatile components of flowers of different peony varieties were clarified, laying a foundation for further molecular biology research into the floral fragrance of peonies and the cultivation of new varieties of aromatic peonies. At the same time, it also provides a theoretical basis for the development and application of peony flower by-products.展开更多
A new monoterpene glycoside, together with nine known ones, 3-O-methylpaeoniflorin, mudanpioside J, paeoniflorin, benzoylpaeoniflorin, oxypaeoniflorin, benzoyloxypaeoniflorin, oxybenzoylpaeoniflorin, albiflorin and la...A new monoterpene glycoside, together with nine known ones, 3-O-methylpaeoniflorin, mudanpioside J, paeoniflorin, benzoylpaeoniflorin, oxypaeoniflorin, benzoyloxypaeoniflorin, oxybenzoylpaeoniflorin, albiflorin and lactiflorin, was isolated from the roots of Paeonia lactiflora Pall.. The structure of the new compound was elucidated as galloylalbiflorin by the spectro- scopic evidence including ESI-MS, 1D- and 2D-NMR spectra.展开更多
Guided by cell-based anti-anaphylactic assay,eighteen cage-like monoterpenoid glycosides(1−18)were obtained from the bioactive fraction of P.lactiflora extract.Among these,compounds 1,5,6,11,12,15,and 17 significantly...Guided by cell-based anti-anaphylactic assay,eighteen cage-like monoterpenoid glycosides(1−18)were obtained from the bioactive fraction of P.lactiflora extract.Among these,compounds 1,5,6,11,12,15,and 17 significantly reduced the release rate ofβ-HEX and HIS without or with less cytotoxicity.Furthermore,the most potent inhibitor benzoylpaeoniflorin(5)was selected as the prioritized compound for the study of action of mechanism,and its anti-anaphylactic activity was medicated by dual-inhibiting HDC and MAPK signal pathway.Moreover,molecular docking simulation explained that benzoylpaeoniflorin(5)blocked the conversion of L-histidine to HIS by occupying the HDC active site.Finally,in vivo on PCA using BALB/c mice,benzoylpaeoniflorin(5)suppressed the IgE-mediated PCA reaction in antigen-challenged mice.These findings indicated that cage-like monoterpenoid glycosides,especially benzoylpaeoniflorin(5),mainly contribute to the anti-anaphylactic activity of P.lactiflora by dual-inhibiting HDC and MAPK signal pathway.Therefore,benzoylpaeoniflorin(5)may be considered as a novel drug candidate for the treatment of anaphylactic diseases.展开更多
Two new neolignans and one new lignan(1-3)were obtained from the roots of Paeonia lactiflora.Their structures were unambiguously elucidated based on extensive spectroscopic analysis,single-crystal X-ray crystallograph...Two new neolignans and one new lignan(1-3)were obtained from the roots of Paeonia lactiflora.Their structures were unambiguously elucidated based on extensive spectroscopic analysis,single-crystal X-ray crystallography,and the calculated and experimental electronic circular dichroism(ECD)spectra.Compound 1 was a racemic mixture and successfully resolved into the anticipated enantiomers via chiral-phase HPLC.Compound 3 demonstrated moderate inhibitory activity against human carboxylesterase 2A1(hCES2Al)with an IC_(50) value of 7.28±0.94 μmol·L^(-1).展开更多
Objective: The analgesic effect of Paeonia Lactiflora has been widely accepted in traditional Chinese medicine. But little is known about the potential mechanism. This study aims to elucidate the effective components ...Objective: The analgesic effect of Paeonia Lactiflora has been widely accepted in traditional Chinese medicine. But little is known about the potential mechanism. This study aims to elucidate the effective components and analgesic mechanism based on network pharmacology. Methods: TCMSP was screened to collect the possible active ingredients and their CAS and SMILES was searched in Pubchem and further be used for reverse molecular docking in Swiss Target Prediction database to obtain potential targets. Pain-related molecules were obtained from GeenCards database, and the predicted targets of Paeonia Lactiflora for pain treatment were selected by Wayne diagram. For mechanism analysis, the protein-protein interactions were constructed by String, the GO analysis and KEGG analysis were conducted in DAVID. Results: Through GO analysis and KEGG analysis, we found that the pain related signaling pathways mainly involved in serotonergic synapse, calcium signaling pathway, inflammatory mediator TRP channels. Using network-based systems biology and molecular docking analyses, we predicted that 11 active ingredients in Paeonia Lactiflora has the analgesic effects with 97 potential targets. PRKCA, CASP3, ALOX15, SLC6A4, PRKCG, ALOX5, PRKCB, ALOX12, EGFR, ADRB2, RYR3, RYR1, NOS2, PTAFR, PRKCQ, and PRKCD were involved in the analgesic effects of Paeonia Lactiflora. Conclusion: Paeonia Lactiflora may alleviate pain through inflammatory mediator regulation of TRP channels, Ca2+ signaling pathway and 5-HT receptor. PRKCA, PRKCB, PRKCD,PRKCQ, and PRKCG may be new targets for pain treatment.展开更多
In order to facilitate the preparation of paeoniflorin(PF)and albiflorin(AF),two chief bioactive constituents in Paeonia lactiflora Pall(PL),induction and culture of callus from PL were studied.With a modified woody p...In order to facilitate the preparation of paeoniflorin(PF)and albiflorin(AF),two chief bioactive constituents in Paeonia lactiflora Pall(PL),induction and culture of callus from PL were studied.With a modified woody plant medium supplemented with 0.5 mg·L-16-benzylaminopurine,1.0 mg·L-1naphthylacetic acid,0.1 mg·L-1thidiazuron and 30 g·L-1sucrose,callus was induced from four kinds of explants:leaf,stems,petiole,and root.The potency to form callus varies between different explants and leaf explants exhibits the highest capacity(100%).On the other hand,root-derived callus(R-callus)produces the highest level of total amount of PF and AF,31.8 mg·g-1dry mass,which is higher than the corresponding level in the root of field cultivated PL.Furthermore,the time needed is only 40 days,remarkably shorter than the cultivation time of PL,about 4–5 years.Higher accumulation levels of PF and AF with shorter production time indicate that callus culture of PL is a promising powerful tool for production of PF and AF in the future.展开更多
Hao Jiang;Yushu Guo;Chao Shi;Department of Pharmacy,Medical Supplies Center of PLA General Hospital;This study aimed to utilize ultra-high-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)to anal...Hao Jiang;Yushu Guo;Chao Shi;Department of Pharmacy,Medical Supplies Center of PLA General Hospital;This study aimed to utilize ultra-high-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)to analyze and quantify eight therapeutic components in Paeonia lactiflora and Glycyrrhiza decoction.The goal was to establish an accurate,efficient,and applicable method for determining the composition of this formula,providing a reference for its clinical application.Methanol was employed to extract the therapeutic components from Paeonia lactiflora and Glycyrrhiza decoction,forming the test sample solution.UHPLC-MS/MS technology was applied to separate and analyze the test samples.The results indicated that upon analysis,the eight reference standards exhibited excellent linear relationships,and the test samples remained stable within 24 h.The content of paeoniflorin,paeonolide,glycyrrhizic acid A,quercetin,chebulic acid,liquiritin,naringenin,and glycyrrhetic acid was determined to be 3.530±0.124,0.139±0.006,0.245±0.008,0.779±0.026,0.433±0.021,0.388±0.018,0.617±0.013,and 0.081±0.005 mg/m L,respectively.This study established that UHPLC-MS/MS analysis demonstrated high efficiency and broad applicability,reflecting the chemical composition characteristics of Paeonia lactiflora and Glycyrrhiza decoction.This research provided a quantitative analysis method for the eight therapeutic components in Paeonia lactiflora and Glycyrrhiza decoction,applicable to its clinical use and quality control.展开更多
The roots of Paeoniae lactiflora Pall.are widely consumed as crude drugs in Asian countries due to their remarkable beneficial health effects.The present research was undertaken to illuminate the dynamic changes in me...The roots of Paeoniae lactiflora Pall.are widely consumed as crude drugs in Asian countries due to their remarkable beneficial health effects.The present research was undertaken to illuminate the dynamic changes in metabolites and enzymes and facilitate selection of the harvesting time when the herb can provide optimum health benefits.P.lactiflora roots were analyzed at 12 stages of growth for monoterpenoid glycosides,phenols,nucleosides,nucleobases,amino acids,and polysaccharides by high-performance liquid chromatography with photodiode array detector,ultra-high pressure liquid chromatography coupled with tandem mass spectrometry,and UV spectrophotometry.The enzyme activities of plant β-glucosidases and esterases were determined by UV methods.The total content of monoterpenoid glycosides and phenols peaked in December.For nucleosides and nucleobases,the highest content appeared in April.The maximum phasic accumulation of the total amino acids took place in March,and the content of total polysaccharides reached a peak value in September.December,April,and March were selected as the appropriate harvesting times for producing natural medicinal or health food products.Plant β-glucosidases and esterases showed the highest activity in December and May,respectively.When the activity of β-glucosidase increased,esterase activity decreased,while the contents of oxypaeoniflora and paeoniflorin increased.When esterase activity increased,the contents of benzoylpaeoniflorin,paeoniflorin,and gallic acid decreased.In conclusion,the results from the present study would be useful in determination of the suitable time for harvesting P.lactiflora roots for medicinal purposes.展开更多
Objective:To study the chemical constituents of the roots of Paeonia lactiflora.Materials and methods:The isolation and purification were carried out by column chromatography on macroporous adsorbent resin,MCI gel,sil...Objective:To study the chemical constituents of the roots of Paeonia lactiflora.Materials and methods:The isolation and purification were carried out by column chromatography on macroporous adsorbent resin,MCI gel,silica gel,and Sephadex LH-20,as well as semi-preparative RP-HPLC.The structures were elucidated on the basis of physicochemical properties and spectroscopic analysis,as well as the ECD quantum chemical computation methods.Results:A sesquiterpenoidal glucoside(1)along with two sesquiterpenoids(2-3)were isolated from the roots of Paeonia lactiflora,and their structures were identified as(+)-(1R,2R,4S,5S,10R)-2-α-D-glucopyranosyloxy-2-hydroxy-cadin-6,12-dien-15-oic acid(1),drim-7-en-3β,11,12-triol(2),and 3β-hydroxy-11,12-O-isopropylidenedrimene(3),respectively.Conclusion:Compound 1 was identified as a new sesquiterpenoidal glucoside.展开更多
Objective To study the chemical constituents from EtOAc extracts of Paeonia lactiflora.Methods Compounds were isolated by various chromatographic techniques and structures were elucidated on the basis of spectral anal...Objective To study the chemical constituents from EtOAc extracts of Paeonia lactiflora.Methods Compounds were isolated by various chromatographic techniques and structures were elucidated on the basis of spectral analysis.Results Seventeen compounds were obtained and their structures were identified as 1,2,6-benzenetriol-1-O-α-D-glucoside(1),paeoniflorin(2),4-methylpaeoniflorin(3),albiflorin(4),paeonidanin(5),benzoylpaeoniflorin(6),4-methylbenzoylpaeoniflorin(7),benzoylalbiflorin(8),paeonidanin A(9),galloylalbiflorin(10),debenzoylalbiflorin(11),4’,5-dihydroxyflavanone-7-O-β-D-glucoside(12),5,7-dihydroxy flavanone-4’-O-β-D-glucoside(13),(+)-catechin(14),gallic acid(15),vanillic acid(16),and 1,2,3-benzenetriol(17).Conclusion Compound 1 is a new compound named paeoniphenoside.Compounds 12 and 13 are firstly obtained from genus Paeonia L.,and compounds 5 and 9 are isolated from P.lactiflora for the first time.展开更多
Objective:To explore the mechanism of paeoniflorin(PF)on osteoarthritis(OA)synovial inflammation from network pharmacology to experimental pharmacology.Methods:Targets of OA were constructed by detecting the database ...Objective:To explore the mechanism of paeoniflorin(PF)on osteoarthritis(OA)synovial inflammation from network pharmacology to experimental pharmacology.Methods:Targets of OA were constructed by detecting the database of network database platforms(Therapeutic Target database,Drug Bank and Gene Cards),and the targets of PF were constructed by Pub Chem and Herbal Ingredients'Targets database.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis of these co-targeted genes were conducted via Database for Annotation,Visualization,and Integrated Discovery(DAVID)database,and protein-protein interaction(PPI)networks were conducted via the search tool for the retrieval of interacting genes(STRING)database.Cell counting kit-8(CCK-8)assay was performed to assess the potential toxicity of PF on human OA fibroblast-like synoviocytes(FLS),quantitative real-time polymerase chain reaction(q PCR),enzyme-linked immunosorbent assay(ELISA)and Western blot were used to verify the potential mechanism of PF in synovial inflammation.Results:Twenty-six co-targeted genes were identified.GO enrichment results showed that these co-targeted genes were most likely localized in the cytoplasm,and the biological processes mainly involved'cellular response to hypoxia''lipopolysaccharide(LPS)-mediated signaling pathway'and'positive regulation of gene expression'.KEGG pathway analysis indicated that these co-targeted genes may function through pathways associated with'hypoxia-inducible factor-1(HIF-1)signaling pathway'and'tumornecrosis factor(TNF)signaling pathway'.The PPI network showed that the top 3 hub genes were TP53,TNF,and CASP3.Molecular docking results showed that PF was well docking with TNF.CCK-8 showed no potential toxicity of 10,20 and 50μmol/L PF on human OA FLS.And PF significantly decreased the expression levels of interleukin-1β,interleukin-6,TNF-αmatrix metalloproteinase 13(MMP13),and a disintegrin and metalloproteinase with thrombospondin motifs 5(ADAMTS5)and TNF-αin LPS-induced OA FLS.Conclusion:PF exhibited potent anti-inflammatory effect in OA synovial inflammation.展开更多
White mugwort (Artemisia lactiflora Wall.) is known as a medicinal herb containing phytochemicals with antioxidant and bio-activity.The optimal concentration of ethanol was investigated for extracting polyphenols from...White mugwort (Artemisia lactiflora Wall.) is known as a medicinal herb containing phytochemicals with antioxidant and bio-activity.The optimal concentration of ethanol was investigated for extracting polyphenols from the fresh aerial part and dried powder of white mugwort as expressed by total phenolic content (TPC),phenolic compounds,antioxidant,anti-inflammatory,and anti-adipogenic activity.Among various ethanol concentrations (0,25,50 or 95%) used in the extraction process,the freeze-dried extract from fresh aerial part (FE) extracted with 95% ethanol expressed the highest antioxidant activity (DPPH radical scavenging IC50 of 0.817 ± 0.06 μl/mg dry weight (DW) and FRAP value of 11.40 ± 0.86 mmol Fe(II)/g DW) and the highest TPC (61.46 ± 3.09 mgGAE/g DW).While,the freeze-dried extract from dried powder (PE) exhibited the prominent antioxidant properties (DPPH radical scavenging IC50 of 0.975 ± 0.03 μl/mg DW and FRAP value of 07.60 ± 0.22 mmol Fe(II)/g DW),TPC (48.11 ± 1.45 mgGAE/g DW) when extracted with 50% ethanol.Seven phenolic compounds—two phenolic acids (gallic acid and tannic acid) and five flavonoids (apigenin,isoquercetin,quercetin,rutin,and catechin) were identified in FE and PE using HPLC-DAD/MS.The inflammatory genes were down-regulated in lipopolysaccharide-stimulated THP-1 monocytes treated with PE,suggesting anti-inflammatory activity.Lipid accumulation in 3T3-L1 adipocytes treated with FE at 60 μ gGAE/mL decreased to 12.8%,while the decrease of 36.8% was detected in PE.Hence,flavonoids were suggested to be responsible for the stronger bioactivity of PE over FE.Moreover,this work originally revealed the investigation of the anti-inflammatory and anti-adipogenic activity of white mugwort on THP-1 and 3T3-L1 cells.展开更多
文摘<i><span style="font-family:Verdana;">Paeonia lactiflora</span></i><span style="font-family:""><span style="font-family:Verdana;"> Pall. var. </span><i><span style="font-family:Verdana;">trichocarpa </span></i><span style="font-family:Verdana;">is a variety of </span><i><span style="font-family:Verdana;">Paeonia lactiflora</span></i><span style="font-family:Verdana;"> Pall., and is currently the peony herb</span></span><span style="font-family:Verdana;">’</span><span style="font-family:""><span style="font-family:Verdana;">s principal cultivar group. Here, we study the differences in aromatic components and flowers of different varieties between two groups of cultivars, providing a reference for applying natural fragrance substances of peonies, breeding fragrant flower types, and developing and </span><span style="font-family:Verdana;">using improved varieties. Headspace solid-phase microextraction (HS-SPME),</span> <span style="font-family:Verdana;">gas chromatography-mass spectrometry (GC-MS), peak area normalization for</span><span style="font-family:Verdana;"> each component relative to content, component library (NIST14/NIST14S) retrieval, and a literature review were used to analyze the volatile compounds in flowers of eight peony varieties, such as </span></span><span style="font-family:Verdana;">“</span><span style="font-family:Verdana;">Gaoganhong</span><span style="font-family:Verdana;">”</span><span style="font-family:Verdana;">, and ten comospore peony varieties, such as </span><span style="font-family:Verdana;">“</span><span style="font-family:Verdana;">Jinshanhong</span><span style="font-family:Verdana;">”</span><span style="font-family:""><span style="font-family:Verdana;">. Results showed that the main volatile compound constituents in flowers of the two groups were terpenes and alcohols. Additionally, the content of eucalyptol, caryophyllene, α-Pinene, citronellol, and 3-Hexen-1-ol, acetate, (Z) was high. Peony cultivars contained linalool,</span><span style="font-family:Verdana;"> (1R)-2,6,6-trimethylbicyclo[3.1.1]hept-2-ene, and 1,4-dimethoxybenzene,</span><span style="font-family:Verdana;"> while comospore peony varieties contained 1,3,6-octatriene, 3,7-dimethyl-, (Z)-, phenylethyl alcohol, and geraniol. In this study, the differences between the volatile components of flowers of different peony varieties were clarified, laying a foundation for further molecular biology research into the floral fragrance of peonies and the cultivation of new varieties of aromatic peonies. At the same time, it also provides a theoretical basis for the development and application of peony flower by-products.
文摘A new monoterpene glycoside, together with nine known ones, 3-O-methylpaeoniflorin, mudanpioside J, paeoniflorin, benzoylpaeoniflorin, oxypaeoniflorin, benzoyloxypaeoniflorin, oxybenzoylpaeoniflorin, albiflorin and lactiflorin, was isolated from the roots of Paeonia lactiflora Pall.. The structure of the new compound was elucidated as galloylalbiflorin by the spectro- scopic evidence including ESI-MS, 1D- and 2D-NMR spectra.
基金This work was supported by the National Natural Science Foundation of China(Nos:81773996,81773589,500101135,and 81522050)Beijing Natural Science Foundation(No.JQ18026).
文摘Guided by cell-based anti-anaphylactic assay,eighteen cage-like monoterpenoid glycosides(1−18)were obtained from the bioactive fraction of P.lactiflora extract.Among these,compounds 1,5,6,11,12,15,and 17 significantly reduced the release rate ofβ-HEX and HIS without or with less cytotoxicity.Furthermore,the most potent inhibitor benzoylpaeoniflorin(5)was selected as the prioritized compound for the study of action of mechanism,and its anti-anaphylactic activity was medicated by dual-inhibiting HDC and MAPK signal pathway.Moreover,molecular docking simulation explained that benzoylpaeoniflorin(5)blocked the conversion of L-histidine to HIS by occupying the HDC active site.Finally,in vivo on PCA using BALB/c mice,benzoylpaeoniflorin(5)suppressed the IgE-mediated PCA reaction in antigen-challenged mice.These findings indicated that cage-like monoterpenoid glycosides,especially benzoylpaeoniflorin(5),mainly contribute to the anti-anaphylactic activity of P.lactiflora by dual-inhibiting HDC and MAPK signal pathway.Therefore,benzoylpaeoniflorin(5)may be considered as a novel drug candidate for the treatment of anaphylactic diseases.
基金supported by the National Natural Science Foundation of China(Nos.81773589 and 82073978)Beijing Natural Science Foundation(No.JQ18026)the Innovation Platform for the Development and Construction of Special Project of Key Laboratory for Tibet Plateau Phytochemistry of Qinghai Province(No.2020-ZJ-Y20).
文摘Two new neolignans and one new lignan(1-3)were obtained from the roots of Paeonia lactiflora.Their structures were unambiguously elucidated based on extensive spectroscopic analysis,single-crystal X-ray crystallography,and the calculated and experimental electronic circular dichroism(ECD)spectra.Compound 1 was a racemic mixture and successfully resolved into the anticipated enantiomers via chiral-phase HPLC.Compound 3 demonstrated moderate inhibitory activity against human carboxylesterase 2A1(hCES2Al)with an IC_(50) value of 7.28±0.94 μmol·L^(-1).
基金the National Natural Science Foundation of China (Grant No. 81874404).
文摘Objective: The analgesic effect of Paeonia Lactiflora has been widely accepted in traditional Chinese medicine. But little is known about the potential mechanism. This study aims to elucidate the effective components and analgesic mechanism based on network pharmacology. Methods: TCMSP was screened to collect the possible active ingredients and their CAS and SMILES was searched in Pubchem and further be used for reverse molecular docking in Swiss Target Prediction database to obtain potential targets. Pain-related molecules were obtained from GeenCards database, and the predicted targets of Paeonia Lactiflora for pain treatment were selected by Wayne diagram. For mechanism analysis, the protein-protein interactions were constructed by String, the GO analysis and KEGG analysis were conducted in DAVID. Results: Through GO analysis and KEGG analysis, we found that the pain related signaling pathways mainly involved in serotonergic synapse, calcium signaling pathway, inflammatory mediator TRP channels. Using network-based systems biology and molecular docking analyses, we predicted that 11 active ingredients in Paeonia Lactiflora has the analgesic effects with 97 potential targets. PRKCA, CASP3, ALOX15, SLC6A4, PRKCG, ALOX5, PRKCB, ALOX12, EGFR, ADRB2, RYR3, RYR1, NOS2, PTAFR, PRKCQ, and PRKCD were involved in the analgesic effects of Paeonia Lactiflora. Conclusion: Paeonia Lactiflora may alleviate pain through inflammatory mediator regulation of TRP channels, Ca2+ signaling pathway and 5-HT receptor. PRKCA, PRKCB, PRKCD,PRKCQ, and PRKCG may be new targets for pain treatment.
基金Supported by the National Natural Science Foundation of China(21176220,31240054)Key Technology Research and Development Project of Ningbo(2011C11023)Zhejiang Provincial Natural Science Foundation of China(Z13B060008)
文摘In order to facilitate the preparation of paeoniflorin(PF)and albiflorin(AF),two chief bioactive constituents in Paeonia lactiflora Pall(PL),induction and culture of callus from PL were studied.With a modified woody plant medium supplemented with 0.5 mg·L-16-benzylaminopurine,1.0 mg·L-1naphthylacetic acid,0.1 mg·L-1thidiazuron and 30 g·L-1sucrose,callus was induced from four kinds of explants:leaf,stems,petiole,and root.The potency to form callus varies between different explants and leaf explants exhibits the highest capacity(100%).On the other hand,root-derived callus(R-callus)produces the highest level of total amount of PF and AF,31.8 mg·g-1dry mass,which is higher than the corresponding level in the root of field cultivated PL.Furthermore,the time needed is only 40 days,remarkably shorter than the cultivation time of PL,about 4–5 years.Higher accumulation levels of PF and AF with shorter production time indicate that callus culture of PL is a promising powerful tool for production of PF and AF in the future.
文摘Hao Jiang;Yushu Guo;Chao Shi;Department of Pharmacy,Medical Supplies Center of PLA General Hospital;This study aimed to utilize ultra-high-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)to analyze and quantify eight therapeutic components in Paeonia lactiflora and Glycyrrhiza decoction.The goal was to establish an accurate,efficient,and applicable method for determining the composition of this formula,providing a reference for its clinical application.Methanol was employed to extract the therapeutic components from Paeonia lactiflora and Glycyrrhiza decoction,forming the test sample solution.UHPLC-MS/MS technology was applied to separate and analyze the test samples.The results indicated that upon analysis,the eight reference standards exhibited excellent linear relationships,and the test samples remained stable within 24 h.The content of paeoniflorin,paeonolide,glycyrrhizic acid A,quercetin,chebulic acid,liquiritin,naringenin,and glycyrrhetic acid was determined to be 3.530±0.124,0.139±0.006,0.245±0.008,0.779±0.026,0.433±0.021,0.388±0.018,0.617±0.013,and 0.081±0.005 mg/m L,respectively.This study established that UHPLC-MS/MS analysis demonstrated high efficiency and broad applicability,reflecting the chemical composition characteristics of Paeonia lactiflora and Glycyrrhiza decoction.This research provided a quantitative analysis method for the eight therapeutic components in Paeonia lactiflora and Glycyrrhiza decoction,applicable to its clinical use and quality control.
基金financially supported by National Natural Science Foundation of China(No.81573555)Natural Science Foundation of Jiangsu Province,China(No.BK 20160097)
文摘The roots of Paeoniae lactiflora Pall.are widely consumed as crude drugs in Asian countries due to their remarkable beneficial health effects.The present research was undertaken to illuminate the dynamic changes in metabolites and enzymes and facilitate selection of the harvesting time when the herb can provide optimum health benefits.P.lactiflora roots were analyzed at 12 stages of growth for monoterpenoid glycosides,phenols,nucleosides,nucleobases,amino acids,and polysaccharides by high-performance liquid chromatography with photodiode array detector,ultra-high pressure liquid chromatography coupled with tandem mass spectrometry,and UV spectrophotometry.The enzyme activities of plant β-glucosidases and esterases were determined by UV methods.The total content of monoterpenoid glycosides and phenols peaked in December.For nucleosides and nucleobases,the highest content appeared in April.The maximum phasic accumulation of the total amino acids took place in March,and the content of total polysaccharides reached a peak value in September.December,April,and March were selected as the appropriate harvesting times for producing natural medicinal or health food products.Plant β-glucosidases and esterases showed the highest activity in December and May,respectively.When the activity of β-glucosidase increased,esterase activity decreased,while the contents of oxypaeoniflora and paeoniflorin increased.When esterase activity increased,the contents of benzoylpaeoniflorin,paeoniflorin,and gallic acid decreased.In conclusion,the results from the present study would be useful in determination of the suitable time for harvesting P.lactiflora roots for medicinal purposes.
基金This research was financially supported by the National Natural Science Foundation of China(NNSFC,Nos.81773589 and 81522050)the National Science and Technology Project of China(No.2018zx09711001-001)the National Key Research and Development Project(No.2019YFC1708901).
文摘Objective:To study the chemical constituents of the roots of Paeonia lactiflora.Materials and methods:The isolation and purification were carried out by column chromatography on macroporous adsorbent resin,MCI gel,silica gel,and Sephadex LH-20,as well as semi-preparative RP-HPLC.The structures were elucidated on the basis of physicochemical properties and spectroscopic analysis,as well as the ECD quantum chemical computation methods.Results:A sesquiterpenoidal glucoside(1)along with two sesquiterpenoids(2-3)were isolated from the roots of Paeonia lactiflora,and their structures were identified as(+)-(1R,2R,4S,5S,10R)-2-α-D-glucopyranosyloxy-2-hydroxy-cadin-6,12-dien-15-oic acid(1),drim-7-en-3β,11,12-triol(2),and 3β-hydroxy-11,12-O-isopropylidenedrimene(3),respectively.Conclusion:Compound 1 was identified as a new sesquiterpenoidal glucoside.
基金Fok Ying Tong Education Foundation,Ministry of Education of China(111046)
文摘Objective To study the chemical constituents from EtOAc extracts of Paeonia lactiflora.Methods Compounds were isolated by various chromatographic techniques and structures were elucidated on the basis of spectral analysis.Results Seventeen compounds were obtained and their structures were identified as 1,2,6-benzenetriol-1-O-α-D-glucoside(1),paeoniflorin(2),4-methylpaeoniflorin(3),albiflorin(4),paeonidanin(5),benzoylpaeoniflorin(6),4-methylbenzoylpaeoniflorin(7),benzoylalbiflorin(8),paeonidanin A(9),galloylalbiflorin(10),debenzoylalbiflorin(11),4’,5-dihydroxyflavanone-7-O-β-D-glucoside(12),5,7-dihydroxy flavanone-4’-O-β-D-glucoside(13),(+)-catechin(14),gallic acid(15),vanillic acid(16),and 1,2,3-benzenetriol(17).Conclusion Compound 1 is a new compound named paeoniphenoside.Compounds 12 and 13 are firstly obtained from genus Paeonia L.,and compounds 5 and 9 are isolated from P.lactiflora for the first time.
基金Supported by the National Natural Science Foundation of China (No.81373662 and No.81874475)Capacity Building Project of Chinese and Western Medicine Clinical Collaboration on Major Difficult Disease (No.201803190106)。
文摘Objective:To explore the mechanism of paeoniflorin(PF)on osteoarthritis(OA)synovial inflammation from network pharmacology to experimental pharmacology.Methods:Targets of OA were constructed by detecting the database of network database platforms(Therapeutic Target database,Drug Bank and Gene Cards),and the targets of PF were constructed by Pub Chem and Herbal Ingredients'Targets database.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis of these co-targeted genes were conducted via Database for Annotation,Visualization,and Integrated Discovery(DAVID)database,and protein-protein interaction(PPI)networks were conducted via the search tool for the retrieval of interacting genes(STRING)database.Cell counting kit-8(CCK-8)assay was performed to assess the potential toxicity of PF on human OA fibroblast-like synoviocytes(FLS),quantitative real-time polymerase chain reaction(q PCR),enzyme-linked immunosorbent assay(ELISA)and Western blot were used to verify the potential mechanism of PF in synovial inflammation.Results:Twenty-six co-targeted genes were identified.GO enrichment results showed that these co-targeted genes were most likely localized in the cytoplasm,and the biological processes mainly involved'cellular response to hypoxia''lipopolysaccharide(LPS)-mediated signaling pathway'and'positive regulation of gene expression'.KEGG pathway analysis indicated that these co-targeted genes may function through pathways associated with'hypoxia-inducible factor-1(HIF-1)signaling pathway'and'tumornecrosis factor(TNF)signaling pathway'.The PPI network showed that the top 3 hub genes were TP53,TNF,and CASP3.Molecular docking results showed that PF was well docking with TNF.CCK-8 showed no potential toxicity of 10,20 and 50μmol/L PF on human OA FLS.And PF significantly decreased the expression levels of interleukin-1β,interleukin-6,TNF-αmatrix metalloproteinase 13(MMP13),and a disintegrin and metalloproteinase with thrombospondin motifs 5(ADAMTS5)and TNF-αin LPS-induced OA FLS.Conclusion:PF exhibited potent anti-inflammatory effect in OA synovial inflammation.
基金funded by Thailand Researchers and Research for Industry Grants(RRi)(Grant No.PHD61I0001)。
文摘White mugwort (Artemisia lactiflora Wall.) is known as a medicinal herb containing phytochemicals with antioxidant and bio-activity.The optimal concentration of ethanol was investigated for extracting polyphenols from the fresh aerial part and dried powder of white mugwort as expressed by total phenolic content (TPC),phenolic compounds,antioxidant,anti-inflammatory,and anti-adipogenic activity.Among various ethanol concentrations (0,25,50 or 95%) used in the extraction process,the freeze-dried extract from fresh aerial part (FE) extracted with 95% ethanol expressed the highest antioxidant activity (DPPH radical scavenging IC50 of 0.817 ± 0.06 μl/mg dry weight (DW) and FRAP value of 11.40 ± 0.86 mmol Fe(II)/g DW) and the highest TPC (61.46 ± 3.09 mgGAE/g DW).While,the freeze-dried extract from dried powder (PE) exhibited the prominent antioxidant properties (DPPH radical scavenging IC50 of 0.975 ± 0.03 μl/mg DW and FRAP value of 07.60 ± 0.22 mmol Fe(II)/g DW),TPC (48.11 ± 1.45 mgGAE/g DW) when extracted with 50% ethanol.Seven phenolic compounds—two phenolic acids (gallic acid and tannic acid) and five flavonoids (apigenin,isoquercetin,quercetin,rutin,and catechin) were identified in FE and PE using HPLC-DAD/MS.The inflammatory genes were down-regulated in lipopolysaccharide-stimulated THP-1 monocytes treated with PE,suggesting anti-inflammatory activity.Lipid accumulation in 3T3-L1 adipocytes treated with FE at 60 μ gGAE/mL decreased to 12.8%,while the decrease of 36.8% was detected in PE.Hence,flavonoids were suggested to be responsible for the stronger bioactivity of PE over FE.Moreover,this work originally revealed the investigation of the anti-inflammatory and anti-adipogenic activity of white mugwort on THP-1 and 3T3-L1 cells.
