Black point is a black discoloration of the grain embryo that reduces the grain quality and commodity grade.Identifying the underlying genetic loci can facilitate the improvement of black point resistance in wheat.Her...Black point is a black discoloration of the grain embryo that reduces the grain quality and commodity grade.Identifying the underlying genetic loci can facilitate the improvement of black point resistance in wheat.Here,262 recombinant inbred lines(RILs)from the cross of Zhongmai 578/Jimai 22 were evaluated for their black point reactions in fve environments.A high-density genetic linkage map of the RIL population was constructed with the wheat 50K single nucleotide polymorphism(SNP)array.Six stable QTLs for black point resistance were detected,QBp.caas-2A,QBp.caas-2B1,QBp.caas-2B2,QBp.caas-2D,QBp.caas-3A,and QBp.caas-5B,which explained 2.1-28.8%of the phenotypic variances.The resistance alleles of QBp.caas-2B1 and QBp.caas-2B2 were contributed by Zhongmai 578 while the others were from Jimai 22.QBp.caas-2B2,QBp.caas-2D and QBp.caas-3A overlapped with previously reported loci,whereas QBp.caas-2A,QBp.caas-2B1 and QBp.caas-5B are likely to be new.Five kompetitive allele-specifc PCR(KASP)markers,Kasp_2A_BP,Kasp_2B1_BP,Kasp_2B2_BP,Kasp_3A_BP,and Kasp_5B_BP,were validated in a natural population of 165 cultivars.The fndings of this study provide useful QTLs and molecular markers for the improvement of black point resistance in wheat through marker-assisted breeding.展开更多
In order to identify the molecular markers that can be widely used in the breeding of Brassica napus L.varieties with high seed oil content under different genetic backgrounds,we developed a Kompetitive Allele Specifi...In order to identify the molecular markers that can be widely used in the breeding of Brassica napus L.varieties with high seed oil content under different genetic backgrounds,we developed a Kompetitive Allele Specific PCR(KASP)marker for seed oil content on the basis of the results from available studies.The verification in the F_(2) population showed that the marker was closely linked to the quantitative trait locus(QTL)for oil content on chromosome A05.The findings helped to breed the‘Fengyou’varieties with high seed oil content in the middle reaches of the Yangtze River.展开更多
Leaf rust is a highly destructive disease that poses a significant threat to both the yield and quality of wheat.Identification of genetic loci can aid in enhancing leaf rust resistance in wheat breeding.In the presen...Leaf rust is a highly destructive disease that poses a significant threat to both the yield and quality of wheat.Identification of genetic loci can aid in enhancing leaf rust resistance in wheat breeding.In the present study,262 recombinant inbred lines derived from a cross between Zhongmai 578 and Jimai 22 were used to map leaf rust resistance loci using the Wheat 50K single-nucleotide polymorphism(SNP)array across four environments.Four quantitative trait loci(QTL)on chromosomes 2B(2),5B,and 7B were identified through composite interval mapping,designated QLr.caas-2B.1,QLr.caas-2B.2,QLr.caas-5B,and QLr.caas-7B,respectively,explaining 3.7%–19.6%of the phenotypic variances.The resistance alleles at QLr.caas-2B.1,QLr.caas-5B,and QLr.caas-7B originated from Zhongmai 578,while that at QLr.caas-2B.2 came from Jimai 22.Both QLr.caas-2B.2 and QLr.caas-5B overlapped with loci previously reported,whereas QLr.caas-2B.1 and QLr.caas-7B are likely to be new loci.Two kompetitive allelespecific PCR(KASP)markers,KASP-QLr.caas-2B.2 and KASP-QLr.caas-5B,were proven to be significantly associated with leaf rust resistance in a diverse panel of 119 wheat varieties mainly from China.Four candidate genes encoding a lectin-receptor kinase protein,F-box family protein,ankyrin repeat domain protein,and putative ABC transporter,respectively,were identified in genetic regions of the four QTL.These findings provide valuable QTL and breeding available KASP markers,facilitating the improvement of leaf rust resistance in wheat through marker-assisted breeding.展开更多
基金funded by the National Natural Science Foundation of China(32272186)the Beijing Natural Science Foundation,China(6242031)+5 种基金the Basal Research Fund of the Chinese Academy of Agricultural Sciences(CAAS)(S2022QH04)the National Key R&D Program of China(2022YFD1201500)the Young Elite Scientists Sponsorship Program by China Association for Science and Technology(YESS,2020QNRC001)the Modern Cold and Drought Characteristic Agricultural Seed Industry Research Project-2025,Gansu Province,China(ZYGG-2025-8)the Nanfan Special Project,CAAS(YBXM2303)the Science and Technology Innovation Program of CAAS。
文摘Black point is a black discoloration of the grain embryo that reduces the grain quality and commodity grade.Identifying the underlying genetic loci can facilitate the improvement of black point resistance in wheat.Here,262 recombinant inbred lines(RILs)from the cross of Zhongmai 578/Jimai 22 were evaluated for their black point reactions in fve environments.A high-density genetic linkage map of the RIL population was constructed with the wheat 50K single nucleotide polymorphism(SNP)array.Six stable QTLs for black point resistance were detected,QBp.caas-2A,QBp.caas-2B1,QBp.caas-2B2,QBp.caas-2D,QBp.caas-3A,and QBp.caas-5B,which explained 2.1-28.8%of the phenotypic variances.The resistance alleles of QBp.caas-2B1 and QBp.caas-2B2 were contributed by Zhongmai 578 while the others were from Jimai 22.QBp.caas-2B2,QBp.caas-2D and QBp.caas-3A overlapped with previously reported loci,whereas QBp.caas-2A,QBp.caas-2B1 and QBp.caas-5B are likely to be new.Five kompetitive allele-specifc PCR(KASP)markers,Kasp_2A_BP,Kasp_2B1_BP,Kasp_2B2_BP,Kasp_3A_BP,and Kasp_5B_BP,were validated in a natural population of 165 cultivars.The fndings of this study provide useful QTLs and molecular markers for the improvement of black point resistance in wheat through marker-assisted breeding.
文摘In order to identify the molecular markers that can be widely used in the breeding of Brassica napus L.varieties with high seed oil content under different genetic backgrounds,we developed a Kompetitive Allele Specific PCR(KASP)marker for seed oil content on the basis of the results from available studies.The verification in the F_(2) population showed that the marker was closely linked to the quantitative trait locus(QTL)for oil content on chromosome A05.The findings helped to breed the‘Fengyou’varieties with high seed oil content in the middle reaches of the Yangtze River.
基金funded by the National Natural Science Foundation of China(32272186 and 32060481)the Beijing Natural Science Foundation(6242031)+4 种基金the modern cold and drought characteristic agricultural seed industry research project-2025(ZYGG-2025-8)the Young Elite Scientists Sponsorship Program by China Association for Science and Technology(YESS,2020QNRC001)the Nanfan Special Project,CAAS(YBXM2303)the National Key R&D Program of China(2022YFD1201500)the CAAS Science and Technology Innovation Program.
文摘Leaf rust is a highly destructive disease that poses a significant threat to both the yield and quality of wheat.Identification of genetic loci can aid in enhancing leaf rust resistance in wheat breeding.In the present study,262 recombinant inbred lines derived from a cross between Zhongmai 578 and Jimai 22 were used to map leaf rust resistance loci using the Wheat 50K single-nucleotide polymorphism(SNP)array across four environments.Four quantitative trait loci(QTL)on chromosomes 2B(2),5B,and 7B were identified through composite interval mapping,designated QLr.caas-2B.1,QLr.caas-2B.2,QLr.caas-5B,and QLr.caas-7B,respectively,explaining 3.7%–19.6%of the phenotypic variances.The resistance alleles at QLr.caas-2B.1,QLr.caas-5B,and QLr.caas-7B originated from Zhongmai 578,while that at QLr.caas-2B.2 came from Jimai 22.Both QLr.caas-2B.2 and QLr.caas-5B overlapped with loci previously reported,whereas QLr.caas-2B.1 and QLr.caas-7B are likely to be new loci.Two kompetitive allelespecific PCR(KASP)markers,KASP-QLr.caas-2B.2 and KASP-QLr.caas-5B,were proven to be significantly associated with leaf rust resistance in a diverse panel of 119 wheat varieties mainly from China.Four candidate genes encoding a lectin-receptor kinase protein,F-box family protein,ankyrin repeat domain protein,and putative ABC transporter,respectively,were identified in genetic regions of the four QTL.These findings provide valuable QTL and breeding available KASP markers,facilitating the improvement of leaf rust resistance in wheat through marker-assisted breeding.
