Based on the loading conditions of engine, applying difference method to solve the hydrodynamic lubrication equation of piston skirt movement, the force acting on piston skirt and the moment on wrist pin were obtained...Based on the loading conditions of engine, applying difference method to solve the hydrodynamic lubrication equation of piston skirt movement, the force acting on piston skirt and the moment on wrist pin were obtained. A computer program for simulating the piston second order motion was conducted to calculate the lateral motion of the upper part and the bottom part of piston skirts of the engine of automotive model CA1091. From the simulated result, the maximal impacting phase and the maximal impacting region of the piston were obtained. The result can be used for designing engine, diagnosing the noise of piston knocking cylinder wall and explaining many practical fault phenomena in theory.展开更多
Pre-Authentication and Post-Connection(PAPC)plays a crucial role in realizing the Zero Trust security model by ensuring that access to network resources is granted only after successful authentication.While earlier ap...Pre-Authentication and Post-Connection(PAPC)plays a crucial role in realizing the Zero Trust security model by ensuring that access to network resources is granted only after successful authentication.While earlier approaches such as Port Knocking(PK)and Single Packet Authorization(SPA)introduced pre-authentication concepts,they suffer from limitations including plaintext communication,protocol dependency,reliance on dedicated clients,and inefficiency under modern network conditions.These constraints hinder their applicability in emerging distributed and resource-constrained environments such as AIoT and browser-based systems.To address these challenges,this study proposes a novel port-sequence-based PAPC scheme structured as a modular model comprising a client,server,and ephemeral Key Management System(KMS).The system employs the Advanced Encryption Standard(AES-128)to protect message confidentiality and uses a Hash-Based Message Authentication Code(HMAC-SHA256)to ensure integrity.Authentication messages are securely fragmented and mapped to destination port numbers using a signature-based avoidance algorithm,which prevents collisions with unsafe or reserved port ranges.The server observes incoming port sequences,retrieves the necessary keys from the KMS,reconstructs and verifies the encrypted data,and conditionally updates firewall policies.Unlike SPA,which requires decrypting all incoming payloads and imposes server-side overhead,the proposed system verifies only port-derived fragments,significantly reducing computational burden.Furthermore,it eliminates the need for raw socket access or custom clients,supporting browser-based operation and enabling protocol-independent deployment.Through a functional web-based prototype and emulated testing,the system achieved an F1-score exceeding 95%in detecting unauthorized access while maintaining low resource overhead.Although port sequence generation introduces some client-side cost,it remains lightweight and scalable.By tightly integrating lightweight cryptographic algorithms with a transport-layer communication model,this work presents a conceptually validated architecture that contributes a novel direction for interoperable and scalable Zero Trust enforcement in future network ecosystems.展开更多
Words are like magic.They can lift you up,or they can knock you down.They are the most powerful tool we have.When I was in Grade 7,I performed badly in a math test.
U.S. Secretary of State Hillary Clinton visits Asia on her first overseas trip, showing Asia’s growing importance in American foreign policy Traditionally, the U.S. secretary of state heads to Europe first, but on he...U.S. Secretary of State Hillary Clinton visits Asia on her first overseas trip, showing Asia’s growing importance in American foreign policy Traditionally, the U.S. secretary of state heads to Europe first, but on her first foreign trip Hillary Clinton flew across the Pacific to visit four Asian countries: Japan, Indonesia,展开更多
A cylinder combustion simulation model was established for a two-stroke aviation piston engine used in a small unmanned aerial vehicle. The influence of different ignition system parameters on the combustion process o...A cylinder combustion simulation model was established for a two-stroke aviation piston engine used in a small unmanned aerial vehicle. The influence of different ignition system parameters on the combustion process of aviation kerosene was studied using this model. The research results showed that under the working conditions of 5500 r/min and 50% throttle opening, as the ignition energy increased, the peak values of average cylinder pressure and average temperature increased, and the combustion duration shortened, The advance of the combustion center of gravity increases the tendency of the engine to knock. Under the same operating conditions, as the ignition timing advances, the peak values of average pressure and average temperature in the cylinder increase, gradually approaching the top dead center, and the tendency of engine detonation increases more significantly.展开更多
Based on the field observation on the parental behavior of Oriental White Stork (Ciconia boyciana) during their brooding period, this paper describes the breeding style, feeding behavior, water chicks, protect chicks,...Based on the field observation on the parental behavior of Oriental White Stork (Ciconia boyciana) during their brooding period, this paper describes the breeding style, feeding behavior, water chicks, protect chicks, etc. A Ritual “behavior—Culmen Knocking/bill clattering” was observed and which is divided into three styles with deferent meanings. This behavior plays an important role during this season.展开更多
[Objective] The aims were to construct a new suicide plasmid of Lactobacillus and gene deletion engineering bacteria of Lactobacillus with pUC19 vector. [Methods] pUC19-CM was constructed by inserting a chloramphenico...[Objective] The aims were to construct a new suicide plasmid of Lactobacillus and gene deletion engineering bacteria of Lactobacillus with pUC19 vector. [Methods] pUC19-CM was constructed by inserting a chloramphenicol resistant gene into the multi-cloning site of pUC19,and then two homologous fragments were cloned into each side of the pUC19-CM to construct suicide plasmid pUC19-CM-D. [Results] A replacement mutant strain,whose target gene was replaced by resistant gene,could be obtained by transforming the suicide plasmid pUC19-CM-D into Lactobacillus for resistance screening. [Conclusion] The construction and application of pUC19-CM-D provided a fast and efficient means of construction of gene deletion engineering bacteria of Lactobacillus,and laid a foundation for study of gene function of Lactobacillus.展开更多
The analysis and design of a large diameter shaft assembly scheme is composed of pneumatic impact hammer, elevator and supporting trolley;Firstly, align the large-diameter shaft with the bearing hole. At first, let th...The analysis and design of a large diameter shaft assembly scheme is composed of pneumatic impact hammer, elevator and supporting trolley;Firstly, align the large-diameter shaft with the bearing hole. At first, let the large-diameter shaft enter a part of the bearing hole, and then knock the large-diameter shaft with a pneumatic impact hammer to install the large-diameter shaft into the bearing of large equipment. Thus, the labor intensity of manual installation is reduced, and the installation accuracy and production efficiency are improved. The design makes the installation of large-diameter shaft very convenient and simple, greatly improves the installation efficiency of large-diameter shaft and reduces the labor time of workers, and provides a very good solution for the installation of large-scale equipment shafts.展开更多
Cylinder pressure fluctuation during combustion process of internal combustion engine is closely related to combustion noise and knock.The current studies are based on cylinder pressure test to obtain information on c...Cylinder pressure fluctuation during combustion process of internal combustion engine is closely related to combustion noise and knock.The current studies are based on cylinder pressure test to obtain information on combustion noise and knock,but there is little for simulation of combustion pressure fluctuation.Based on effects of combustion process in the combustion chamber on cylinder pressure by using wave equation,the mechanism of pressure fluctuation during combustion is researched with three-dimensional acoustic wave equation and flow field model of KIVA program.The cylinder pressure fluctuation curve,temperature field and acoustic field are obtained from the coupled calculation of the wave equation and KIVA program.The frequency spectrum analysis is taken with the cylinder pressure oscillation of cylinder pressure measured and calculated.The calculation result is consistent with the experimental result.This indicates that the cylinder pressure fluctuation can be correctly calculated with the wave equation.Analysis of calculation results of temperature field and acoustic field shows that sound field changes faster than flame propagates,and distribution of sound field is more complicated.Combustion pressure oscillation in the diesel engine is under highly unstable condition.This indicates that the combination of cylinder pressure fluctuation model and combustion model is an effective method to study the pressure oscillations and a new method to study the combustion noise and knock.展开更多
FAM20A has been studied to a very limited extent. Mutations in human FAM20A cause amelogenesis imperfecta, gingival fibromatosis and kidney problems. It would be desirable to systemically analyse the expression of FAM...FAM20A has been studied to a very limited extent. Mutations in human FAM20A cause amelogenesis imperfecta, gingival fibromatosis and kidney problems. It would be desirable to systemically analyse the expression of FAM20A in dental tissues and to assess the pathological changes when this molecule is specifically nullified in individual tissues. Recently, we generated mice with a Fam2OA-floxed allele containing the beta-galactosidase reporter gene. We analysed FAM20A expression in dental tissues using X-Gal staining, immunohistochemistry and in situ hybridization, which showed that the ameloblasts in the mouse mandibular first molar began to express FAM20A at 1 day after birth, and the reduced enamel epithelium in erupting molars expressed a significant level of FAM2OA. By breeding K14-Cre mice with Fam20An^x/fl^x mice, we created K14-Cre;Fam20Af/flox/flox （conditional knock out, cKO） mice, in which Fam20A was inactivated in the epithelium. We analysed the dental tissues of cKO mice using X-ray radiography： histology and immunohistochemistry. The molar enamel matrix in cKO mice was much thinner than normal and was often separated from the dentinoenamel junction. The Fam2OA-deficient ameloblasts were non-polarized and disorganized and were detached from the enamel matrix. The enamel abnormality in cKO mice was consistent with the diagnosis of amelogenesis imperfecta. The levels of enamelin and matrix metalloproteinase 20 were lower in the ameloblasts and enamel of cKO mice than the normal mice, The cKO mice had remarkable delays in the eruption of molars and hyperplasia of the gingival epithelium. The findings emphasize the essential roles of FAM20A in the development of dental and oral tissues.展开更多
Some new theory and algorithms on wavelet analysis are proposed, including continuous wavelet transform （CWT）, discrete wavelet transform （DWT）, wavelet package transform （WPT）, wavelet denosing and mother wavel...Some new theory and algorithms on wavelet analysis are proposed, including continuous wavelet transform （CWT）, discrete wavelet transform （DWT）, wavelet package transform （WPT）, wavelet denosing and mother wavelet selection, etc. Using the component-based hierarchy mode, the platform for virtual instrument （VI） is constructed, and the functions such as data sampling, data analysis and data present, etc are provided. Subsequently, the wavelet analysis library is designed and developed. The library consists of expert system, experienced database, development platform and abundant wavelet analysis functional module, which together implement general and special wavelet analysis in the field of mechanical engineering, energy source, transportation and biomedicine, etc. Finally, the wavelet analysis virtual instrument library is applied to detect fault called engine knock. Experimental result indicates that the wavelet analysis virtual instrument library can efficiently solve the engineering problem such as detecting engine knock.展开更多
Aviation heavy-fuel spark ignition(SI)piston engines have been paid more and more attention in the area of small aviation.Aviation heavy-fuel refers to aviation kerosene or light diesel fuel,which is safer to use and ...Aviation heavy-fuel spark ignition(SI)piston engines have been paid more and more attention in the area of small aviation.Aviation heavy-fuel refers to aviation kerosene or light diesel fuel,which is safer to use and store compared to gasoline fuel.And diesel fuel is more suitable for small aviation application on land.In this study,numerical simulation was performed to evaluate the possibility of switching from gasoline direct injection spark ignition(DISI)to diesel DISI combustion.Diesel was injected into the cylinder by original DI system and ignited by spark.In the simulation,computational models were calibrated by test data from a DI engine.Based on the calibrated models,furthermore,the behavior of diesel DISI combustion was investigated.The results indicate that diesel DISI combustion is slower compared to gasoline,and the knock tendency of diesel in SI combustion is higher.For a diesel/air mixture with an equivalence ratio of 0.6 to 1.4,higher combustion pressure and faster burning rate occur when the equivalence ratios are 1.2 and 1.0,but the latter has a higher possibility of knock.In summary,the SI combustion of diesel fuel with a rich mixture can achieve better combustion performance in the engine.展开更多
A hybrid of ensemble empirical mode decomposition and empirical mode decomposition (EEMD-EMD) is introduced to diagnose the valve-slap vibration signal,which is relative to the dominant combustion knock vibration sign...A hybrid of ensemble empirical mode decomposition and empirical mode decomposition (EEMD-EMD) is introduced to diagnose the valve-slap vibration signal,which is relative to the dominant combustion knock vibration signal given out by a diesel engine around the top dead center (TDC).The time-frequency representations of intrinsic mode functions (IMFs) decomposed by EEMD-EMD are obtained by adaptive generalized S transform (AGST).A type 493 diesel engine was used for the experiment,and the result indicates that the valve-slap of the diesel engine is serious,and the vibration frequencies are higher than the combustion knock.With EEMD-EMD-AGST approach,the valve-slap can be identified by the vibration analysis of the diesel engine.展开更多
AIM: Portal hypertension is a common complication of liver cirrhosis and significantly increases mortality and morbidity.Previous reports have suggested that the compound thalidomide attenuates portal hypertension(PHT...AIM: Portal hypertension is a common complication of liver cirrhosis and significantly increases mortality and morbidity.Previous reports have suggested that the compound thalidomide attenuates portal hypertension(PHT).However, the mechanism for this action is not fully elucidated.One hypothesis is that thalidomide destabilizes tumor necrosis factor α(TNFα) mR NA and therefore diminishes TNFα induction of nitric oxide synthase(NOS) and the production of nitric oxide(NO).To examine this hypothesis, we utilized the murine partial portal vein ligation(PVL) PHT model in combination with endothelial or inducible NOS isoform gene knockout mice.METHODS: Wild type, inducible nitric oxide synthase(i NOS)-/- and endothelial nitric oxide synthase(e NOS)-/-mice received either PVL or sham surgery and were given either thalidomide or vehicle.Serum nitrate(total nitrate, NOx) was measured daily for 7 d as a surrogate of NO synthesis.Serum TNFα level was quantified by enzyme-linked immunosorbent assay.TNFα m RNA was quantified in liver and aorta tissue by reverse transcription-polymerase chain reaction.PHT was determined by recording splenic pulp pressure(SPP) and abdominal aortic flow after 0-7 d.Response to thalidomide was determined by measurement of SPP and mean arterial pressure(MAP).RESULTS: SPP, abdominal aortic flow(Qao) and plasma NOx were increased in wild type and i NOS-/-PVL mice when compared to sham operated control mice.In contrast, SPP, Qao and plasma NOx were not increased in e NOS-/- PVL mice when compared to sham controls.Serum TNFα level in both sham and PVL mice was below the detection limit of the commercial ELISA used.Therefore, the effect of thalidomide on serum TNFα levels was undetermined in wild type, e NOS-/-or i NOS-/- mice.Thalidomide acutely increased plasma NOx in wild type and e NOS-/- mice but not i NOS-/-mice.Moreover, thalidomide temporarily(0-90 min) decreased mean arterial pressure, SPP and Qao in wild type, e NOS-/- and i NOS-/- PVL mice, after which time levels returned to the respective baseline.CONCLUSION: Thalidomide does not reduce portalpressure in the murine PVL model by modulation of NO biosynthesis.Rather, thalidomide reduces PHT by decreasing MAP by an undetermined mechanism.展开更多
This study investigated the effects of SIRT1 gene knock-out on osteoarthritis in mice, and the possible roles of SREBP2 protein and the PI3K/AKT signaling pathway in the effects. Mice were randomly divided into a norm...This study investigated the effects of SIRT1 gene knock-out on osteoarthritis in mice, and the possible roles of SREBP2 protein and the PI3K/AKT signaling pathway in the effects. Mice were randomly divided into a normal group and a SIRT1 gene knock-out group（6 mice in each group）. In these groups, one side of the knee anterior cruciate ligament was traversed, and the ipsilateral medial meniscus was cut to establish an osteoarthritis model of knee joint. The countralateral synovial bursa was cut out, serving as controls. The knee joint specimens were then divided into four groups： SIRT1^（＋/＋） control group（group A, n=6）; SIRT1^（＋/＋） osteoarthritis group（group B, n=6）; SIRT1^（–/–） control group（group C, n=6）; SIRT1^（–/–） osteoarthritis group（group D, n=6）. HE staining, Masson staining, Safranin O-Fast Green staining and Van Gieson staining were used to observe the morphological changes in the articular cartilage of the knee. Immunohistochemical staining was employed to detect the expression of SIRT1, SREBP2, VEGF, AKT, HMGCR and type Ⅱ collagen proteins. SA-β-gal staining was utilized to evaluate chondrocyte aging. The results showed clear knee joint cartilage destruction and degeneration in the SIRT1^（–/–） osteoarthritis group. The tidal line was twisted and displaced anteriorly. Type Ⅱ collagen was destroyed and distributed unevenly. Compared with the SIRT1^（＋/＋） osteoarthritis group and SIRT1^（–/–） control group, SIRT1 protein expression was not obviously changed in the SIRT1^（–/–） osteoarthritis group（P〉0.05）, while the expression levels of the SREBP2, VEGF and HMGCR proteins were significantly increased（P〈0.05） and the levels of AKT and type Ⅱ collagen proteins were significantly decreased（P〈0.05）. SIRT1 gene knock-out may aggravate cartilage degeneration in osteoarthritis by activating the SREBP2 protein-mediated PI3K/AKT signalling pathway, suggesting that SIRT1 gene may play a protective role against osteoarthritis.展开更多
Knock in spark-ignition（SI） engines severely limits engine performance and thermal efficiency. The researches on knock of downsized SI engine have mainly focused on structural design, performance optimization and ad...Knock in spark-ignition（SI） engines severely limits engine performance and thermal efficiency. The researches on knock of downsized SI engine have mainly focused on structural design, performance optimization and advanced combustion modes, however there is little for simulation study on the effect of cooled exhaust gas recirculation（EGR） combined with downsizing technologies on SI engine performance. On the basis of mean pressure and oscillating pressure during combustion process, the effect of different levels of cooled EGR ratio, supercharging and compression ratio on engine dynamic and knock characteristic is researched with three- dimensional KIVA-3V program coupled with pressure wave equation. The cylinder pressure, combustion temperature, ignition delay timing, combustion duration, maximum mean pressure, and maximum oscillating pressure at different initial conditions are discussed and analyzed to investigate potential approaches to inhibiting engine knock while improving power output. The calculation results of the effect of just cooled EGR on knock characteristic show that appropriate levels of cooled EGR ratio can effectively suppress cylinder high-frequency pressure oscillations without obvious decrease in mean pressure. Analysis of the synergistic effect of cooled EGR, supercharging and compression ratio on knock characteristic indicates that under the condition of high supercharging and compression ratio, several times more cooled EGR ratio than that under the original condition is necessarily utilized to suppress knock occurrence effectively. The proposed method of synergistic effect of cooled EGR and downsizing technologies on knock characteristic, analyzed from the aspects of mean pressure and oscillating pressure, is an effective way to study downsized SI engine knock and provides knock inhibition approaches in practical engineering.展开更多
Meiosis-arrest female 1（MARF1） is a recently identified key oogenic regulator essential for the maintenance of female fertility and genome integrity in mice. However, the detailed functions and the underlying mechan...Meiosis-arrest female 1（MARF1） is a recently identified key oogenic regulator essential for the maintenance of female fertility and genome integrity in mice. However, the detailed functions and the underlying mechanisms of MARF1 remain elusive. Here, in an attempt to create a mouse model expressing fluorescent protein-tagged MARF1 to facilitate further exploration of the roles of MARF1 in oocytes, we produced a Marf1-eGFP knockin（KI） mouse line in which the C-terminal structure and function of MARF1 were interfered by its fusing eGFP peptide. Using these Marfl-eGFP-KI mice, we revealed, unexpectedly, the functions of MARF1 in the control of oocyte meiotic division.We found that the Marfl-eGFP-KI females ovulated mature oocytes with severe meiotic and developmental defects,and thus were infertile. Moreover, meiotic reinitiation was delayed while meiotic completion was accelerated in the KI-oocytes, which was coincident with the increased incidence of oocyte aneuploidy. Therefore, MARF1 is indispensable for maintaining the fidelity of homolog segregation during oocyte maturation, and this function relies on its C-terminal domains.展开更多
文摘Based on the loading conditions of engine, applying difference method to solve the hydrodynamic lubrication equation of piston skirt movement, the force acting on piston skirt and the moment on wrist pin were obtained. A computer program for simulating the piston second order motion was conducted to calculate the lateral motion of the upper part and the bottom part of piston skirts of the engine of automotive model CA1091. From the simulated result, the maximal impacting phase and the maximal impacting region of the piston were obtained. The result can be used for designing engine, diagnosing the noise of piston knocking cylinder wall and explaining many practical fault phenomena in theory.
基金supported by Institute for Information&Communications Technology Planning&Evaluation(IITP)grant funded by the Korea government(MSIT)(No.RS-2022-II221200)Convergence Security Core Talent Training Business(Chungnam National University).
文摘Pre-Authentication and Post-Connection(PAPC)plays a crucial role in realizing the Zero Trust security model by ensuring that access to network resources is granted only after successful authentication.While earlier approaches such as Port Knocking(PK)and Single Packet Authorization(SPA)introduced pre-authentication concepts,they suffer from limitations including plaintext communication,protocol dependency,reliance on dedicated clients,and inefficiency under modern network conditions.These constraints hinder their applicability in emerging distributed and resource-constrained environments such as AIoT and browser-based systems.To address these challenges,this study proposes a novel port-sequence-based PAPC scheme structured as a modular model comprising a client,server,and ephemeral Key Management System(KMS).The system employs the Advanced Encryption Standard(AES-128)to protect message confidentiality and uses a Hash-Based Message Authentication Code(HMAC-SHA256)to ensure integrity.Authentication messages are securely fragmented and mapped to destination port numbers using a signature-based avoidance algorithm,which prevents collisions with unsafe or reserved port ranges.The server observes incoming port sequences,retrieves the necessary keys from the KMS,reconstructs and verifies the encrypted data,and conditionally updates firewall policies.Unlike SPA,which requires decrypting all incoming payloads and imposes server-side overhead,the proposed system verifies only port-derived fragments,significantly reducing computational burden.Furthermore,it eliminates the need for raw socket access or custom clients,supporting browser-based operation and enabling protocol-independent deployment.Through a functional web-based prototype and emulated testing,the system achieved an F1-score exceeding 95%in detecting unauthorized access while maintaining low resource overhead.Although port sequence generation introduces some client-side cost,it remains lightweight and scalable.By tightly integrating lightweight cryptographic algorithms with a transport-layer communication model,this work presents a conceptually validated architecture that contributes a novel direction for interoperable and scalable Zero Trust enforcement in future network ecosystems.
文摘Words are like magic.They can lift you up,or they can knock you down.They are the most powerful tool we have.When I was in Grade 7,I performed badly in a math test.
文摘U.S. Secretary of State Hillary Clinton visits Asia on her first overseas trip, showing Asia’s growing importance in American foreign policy Traditionally, the U.S. secretary of state heads to Europe first, but on her first foreign trip Hillary Clinton flew across the Pacific to visit four Asian countries: Japan, Indonesia,
文摘A cylinder combustion simulation model was established for a two-stroke aviation piston engine used in a small unmanned aerial vehicle. The influence of different ignition system parameters on the combustion process of aviation kerosene was studied using this model. The research results showed that under the working conditions of 5500 r/min and 50% throttle opening, as the ignition energy increased, the peak values of average cylinder pressure and average temperature increased, and the combustion duration shortened, The advance of the combustion center of gravity increases the tendency of the engine to knock. Under the same operating conditions, as the ignition timing advances, the peak values of average pressure and average temperature in the cylinder increase, gradually approaching the top dead center, and the tendency of engine detonation increases more significantly.
文摘Based on the field observation on the parental behavior of Oriental White Stork (Ciconia boyciana) during their brooding period, this paper describes the breeding style, feeding behavior, water chicks, protect chicks, etc. A Ritual “behavior—Culmen Knocking/bill clattering” was observed and which is divided into three styles with deferent meanings. This behavior plays an important role during this season.
基金Supported by National Science &Technology Pillar Program in the Eleventh Five-year Plan Period (2007BAD75B06)Guangxi Sci-ence Foundation (0782003-4)~~
文摘[Objective] The aims were to construct a new suicide plasmid of Lactobacillus and gene deletion engineering bacteria of Lactobacillus with pUC19 vector. [Methods] pUC19-CM was constructed by inserting a chloramphenicol resistant gene into the multi-cloning site of pUC19,and then two homologous fragments were cloned into each side of the pUC19-CM to construct suicide plasmid pUC19-CM-D. [Results] A replacement mutant strain,whose target gene was replaced by resistant gene,could be obtained by transforming the suicide plasmid pUC19-CM-D into Lactobacillus for resistance screening. [Conclusion] The construction and application of pUC19-CM-D provided a fast and efficient means of construction of gene deletion engineering bacteria of Lactobacillus,and laid a foundation for study of gene function of Lactobacillus.
文摘The analysis and design of a large diameter shaft assembly scheme is composed of pneumatic impact hammer, elevator and supporting trolley;Firstly, align the large-diameter shaft with the bearing hole. At first, let the large-diameter shaft enter a part of the bearing hole, and then knock the large-diameter shaft with a pneumatic impact hammer to install the large-diameter shaft into the bearing of large equipment. Thus, the labor intensity of manual installation is reduced, and the installation accuracy and production efficiency are improved. The design makes the installation of large-diameter shaft very convenient and simple, greatly improves the installation efficiency of large-diameter shaft and reduces the labor time of workers, and provides a very good solution for the installation of large-scale equipment shafts.
基金supported by National Natural Science Foundation of China (Grant No. 50706032 and No. 50476065)PhD Programs Foundation of Ministry of Education of China (Grant No. 20070056011)
文摘Cylinder pressure fluctuation during combustion process of internal combustion engine is closely related to combustion noise and knock.The current studies are based on cylinder pressure test to obtain information on combustion noise and knock,but there is little for simulation of combustion pressure fluctuation.Based on effects of combustion process in the combustion chamber on cylinder pressure by using wave equation,the mechanism of pressure fluctuation during combustion is researched with three-dimensional acoustic wave equation and flow field model of KIVA program.The cylinder pressure fluctuation curve,temperature field and acoustic field are obtained from the coupled calculation of the wave equation and KIVA program.The frequency spectrum analysis is taken with the cylinder pressure oscillation of cylinder pressure measured and calculated.The calculation result is consistent with the experimental result.This indicates that the cylinder pressure fluctuation can be correctly calculated with the wave equation.Analysis of calculation results of temperature field and acoustic field shows that sound field changes faster than flame propagates,and distribution of sound field is more complicated.Combustion pressure oscillation in the diesel engine is under highly unstable condition.This indicates that the combination of cylinder pressure fluctuation model and combustion model is an effective method to study the pressure oscillations and a new method to study the combustion noise and knock.
基金supported by the National Natural Science Foundation of China (Grant No. 81171744)the Natural Science Foundation of Heilongjiang Province of China (Grant H201418)
文摘FAM20A has been studied to a very limited extent. Mutations in human FAM20A cause amelogenesis imperfecta, gingival fibromatosis and kidney problems. It would be desirable to systemically analyse the expression of FAM20A in dental tissues and to assess the pathological changes when this molecule is specifically nullified in individual tissues. Recently, we generated mice with a Fam2OA-floxed allele containing the beta-galactosidase reporter gene. We analysed FAM20A expression in dental tissues using X-Gal staining, immunohistochemistry and in situ hybridization, which showed that the ameloblasts in the mouse mandibular first molar began to express FAM20A at 1 day after birth, and the reduced enamel epithelium in erupting molars expressed a significant level of FAM2OA. By breeding K14-Cre mice with Fam20An^x/fl^x mice, we created K14-Cre;Fam20Af/flox/flox （conditional knock out, cKO） mice, in which Fam20A was inactivated in the epithelium. We analysed the dental tissues of cKO mice using X-ray radiography： histology and immunohistochemistry. The molar enamel matrix in cKO mice was much thinner than normal and was often separated from the dentinoenamel junction. The Fam2OA-deficient ameloblasts were non-polarized and disorganized and were detached from the enamel matrix. The enamel abnormality in cKO mice was consistent with the diagnosis of amelogenesis imperfecta. The levels of enamelin and matrix metalloproteinase 20 were lower in the ameloblasts and enamel of cKO mice than the normal mice, The cKO mice had remarkable delays in the eruption of molars and hyperplasia of the gingival epithelium. The findings emphasize the essential roles of FAM20A in the development of dental and oral tissues.
基金This project is supported by National Natural Science Foundation of China(No.50575233).
文摘Some new theory and algorithms on wavelet analysis are proposed, including continuous wavelet transform （CWT）, discrete wavelet transform （DWT）, wavelet package transform （WPT）, wavelet denosing and mother wavelet selection, etc. Using the component-based hierarchy mode, the platform for virtual instrument （VI） is constructed, and the functions such as data sampling, data analysis and data present, etc are provided. Subsequently, the wavelet analysis library is designed and developed. The library consists of expert system, experienced database, development platform and abundant wavelet analysis functional module, which together implement general and special wavelet analysis in the field of mechanical engineering, energy source, transportation and biomedicine, etc. Finally, the wavelet analysis virtual instrument library is applied to detect fault called engine knock. Experimental result indicates that the wavelet analysis virtual instrument library can efficiently solve the engineering problem such as detecting engine knock.
基金Project(2018JJ2041)supported by the Science and Technology Project in Hunan Province,ChinaProject(szjj2019-008)supported by the Open Research Subject of Key Laboratory of Fluid and Power Machinery,Ministry of Education,China。
文摘Aviation heavy-fuel spark ignition(SI)piston engines have been paid more and more attention in the area of small aviation.Aviation heavy-fuel refers to aviation kerosene or light diesel fuel,which is safer to use and store compared to gasoline fuel.And diesel fuel is more suitable for small aviation application on land.In this study,numerical simulation was performed to evaluate the possibility of switching from gasoline direct injection spark ignition(DISI)to diesel DISI combustion.Diesel was injected into the cylinder by original DI system and ignited by spark.In the simulation,computational models were calibrated by test data from a DI engine.Based on the calibrated models,furthermore,the behavior of diesel DISI combustion was investigated.The results indicate that diesel DISI combustion is slower compared to gasoline,and the knock tendency of diesel in SI combustion is higher.For a diesel/air mixture with an equivalence ratio of 0.6 to 1.4,higher combustion pressure and faster burning rate occur when the equivalence ratios are 1.2 and 1.0,but the latter has a higher possibility of knock.In summary,the SI combustion of diesel fuel with a rich mixture can achieve better combustion performance in the engine.
基金Supported by National Key Technology Research and Development Program of China (No.2011BAE22B05)
文摘A hybrid of ensemble empirical mode decomposition and empirical mode decomposition (EEMD-EMD) is introduced to diagnose the valve-slap vibration signal,which is relative to the dominant combustion knock vibration signal given out by a diesel engine around the top dead center (TDC).The time-frequency representations of intrinsic mode functions (IMFs) decomposed by EEMD-EMD are obtained by adaptive generalized S transform (AGST).A type 493 diesel engine was used for the experiment,and the result indicates that the valve-slap of the diesel engine is serious,and the vibration frequencies are higher than the combustion knock.With EEMD-EMD-AGST approach,the valve-slap can be identified by the vibration analysis of the diesel engine.
基金Supported by The Departments of Surgery at the University of Rochester and Indiana University
文摘AIM: Portal hypertension is a common complication of liver cirrhosis and significantly increases mortality and morbidity.Previous reports have suggested that the compound thalidomide attenuates portal hypertension(PHT).However, the mechanism for this action is not fully elucidated.One hypothesis is that thalidomide destabilizes tumor necrosis factor α(TNFα) mR NA and therefore diminishes TNFα induction of nitric oxide synthase(NOS) and the production of nitric oxide(NO).To examine this hypothesis, we utilized the murine partial portal vein ligation(PVL) PHT model in combination with endothelial or inducible NOS isoform gene knockout mice.METHODS: Wild type, inducible nitric oxide synthase(i NOS)-/- and endothelial nitric oxide synthase(e NOS)-/-mice received either PVL or sham surgery and were given either thalidomide or vehicle.Serum nitrate(total nitrate, NOx) was measured daily for 7 d as a surrogate of NO synthesis.Serum TNFα level was quantified by enzyme-linked immunosorbent assay.TNFα m RNA was quantified in liver and aorta tissue by reverse transcription-polymerase chain reaction.PHT was determined by recording splenic pulp pressure(SPP) and abdominal aortic flow after 0-7 d.Response to thalidomide was determined by measurement of SPP and mean arterial pressure(MAP).RESULTS: SPP, abdominal aortic flow(Qao) and plasma NOx were increased in wild type and i NOS-/-PVL mice when compared to sham operated control mice.In contrast, SPP, Qao and plasma NOx were not increased in e NOS-/- PVL mice when compared to sham controls.Serum TNFα level in both sham and PVL mice was below the detection limit of the commercial ELISA used.Therefore, the effect of thalidomide on serum TNFα levels was undetermined in wild type, e NOS-/-or i NOS-/- mice.Thalidomide acutely increased plasma NOx in wild type and e NOS-/- mice but not i NOS-/-mice.Moreover, thalidomide temporarily(0-90 min) decreased mean arterial pressure, SPP and Qao in wild type, e NOS-/- and i NOS-/- PVL mice, after which time levels returned to the respective baseline.CONCLUSION: Thalidomide does not reduce portalpressure in the murine PVL model by modulation of NO biosynthesis.Rather, thalidomide reduces PHT by decreasing MAP by an undetermined mechanism.
基金supported by grants from the National Natural Science Foundation of China(No.81272032)the Research and Development Projects of Shenzhen of China(Nos.JCYJ20150403091443275)
文摘This study investigated the effects of SIRT1 gene knock-out on osteoarthritis in mice, and the possible roles of SREBP2 protein and the PI3K/AKT signaling pathway in the effects. Mice were randomly divided into a normal group and a SIRT1 gene knock-out group（6 mice in each group）. In these groups, one side of the knee anterior cruciate ligament was traversed, and the ipsilateral medial meniscus was cut to establish an osteoarthritis model of knee joint. The countralateral synovial bursa was cut out, serving as controls. The knee joint specimens were then divided into four groups： SIRT1^（＋/＋） control group（group A, n=6）; SIRT1^（＋/＋） osteoarthritis group（group B, n=6）; SIRT1^（–/–） control group（group C, n=6）; SIRT1^（–/–） osteoarthritis group（group D, n=6）. HE staining, Masson staining, Safranin O-Fast Green staining and Van Gieson staining were used to observe the morphological changes in the articular cartilage of the knee. Immunohistochemical staining was employed to detect the expression of SIRT1, SREBP2, VEGF, AKT, HMGCR and type Ⅱ collagen proteins. SA-β-gal staining was utilized to evaluate chondrocyte aging. The results showed clear knee joint cartilage destruction and degeneration in the SIRT1^（–/–） osteoarthritis group. The tidal line was twisted and displaced anteriorly. Type Ⅱ collagen was destroyed and distributed unevenly. Compared with the SIRT1^（＋/＋） osteoarthritis group and SIRT1^（–/–） control group, SIRT1 protein expression was not obviously changed in the SIRT1^（–/–） osteoarthritis group（P〉0.05）, while the expression levels of the SREBP2, VEGF and HMGCR proteins were significantly increased（P〈0.05） and the levels of AKT and type Ⅱ collagen proteins were significantly decreased（P〈0.05）. SIRT1 gene knock-out may aggravate cartilage degeneration in osteoarthritis by activating the SREBP2 protein-mediated PI3K/AKT signalling pathway, suggesting that SIRT1 gene may play a protective role against osteoarthritis.
基金supported by National Natural Science Foundation of China(Grant No. 51176138)Tianjin Municipal Natural Science Foundation of China(Grant No. 12TJZDTJ28800)
文摘Knock in spark-ignition（SI） engines severely limits engine performance and thermal efficiency. The researches on knock of downsized SI engine have mainly focused on structural design, performance optimization and advanced combustion modes, however there is little for simulation study on the effect of cooled exhaust gas recirculation（EGR） combined with downsizing technologies on SI engine performance. On the basis of mean pressure and oscillating pressure during combustion process, the effect of different levels of cooled EGR ratio, supercharging and compression ratio on engine dynamic and knock characteristic is researched with three- dimensional KIVA-3V program coupled with pressure wave equation. The cylinder pressure, combustion temperature, ignition delay timing, combustion duration, maximum mean pressure, and maximum oscillating pressure at different initial conditions are discussed and analyzed to investigate potential approaches to inhibiting engine knock while improving power output. The calculation results of the effect of just cooled EGR on knock characteristic show that appropriate levels of cooled EGR ratio can effectively suppress cylinder high-frequency pressure oscillations without obvious decrease in mean pressure. Analysis of the synergistic effect of cooled EGR, supercharging and compression ratio on knock characteristic indicates that under the condition of high supercharging and compression ratio, several times more cooled EGR ratio than that under the original condition is necessarily utilized to suppress knock occurrence effectively. The proposed method of synergistic effect of cooled EGR and downsizing technologies on knock characteristic, analyzed from the aspects of mean pressure and oscillating pressure, is an effective way to study downsized SI engine knock and provides knock inhibition approaches in practical engineering.
基金supported by National Basic Research Program (973) from Ministry of Science and Technology of the People's Republic of China (grant numbers 2014CB943200, 2013CB945500)National NaturalScience Foundation of China (grant numbers 31471351, 31271538)Natural Science Foundation of Jiangsu Province (grant number BK20140061)to YQ Su
文摘Meiosis-arrest female 1（MARF1） is a recently identified key oogenic regulator essential for the maintenance of female fertility and genome integrity in mice. However, the detailed functions and the underlying mechanisms of MARF1 remain elusive. Here, in an attempt to create a mouse model expressing fluorescent protein-tagged MARF1 to facilitate further exploration of the roles of MARF1 in oocytes, we produced a Marf1-eGFP knockin（KI） mouse line in which the C-terminal structure and function of MARF1 were interfered by its fusing eGFP peptide. Using these Marfl-eGFP-KI mice, we revealed, unexpectedly, the functions of MARF1 in the control of oocyte meiotic division.We found that the Marfl-eGFP-KI females ovulated mature oocytes with severe meiotic and developmental defects,and thus were infertile. Moreover, meiotic reinitiation was delayed while meiotic completion was accelerated in the KI-oocytes, which was coincident with the increased incidence of oocyte aneuploidy. Therefore, MARF1 is indispensable for maintaining the fidelity of homolog segregation during oocyte maturation, and this function relies on its C-terminal domains.
