Fruit quality traits play an important role in consumption of kiwiberry(Actinidia arguta).The genetic basis of fruit quality traits in this woody,perennial and dioecious fruit crop remains largely unknown.This study a...Fruit quality traits play an important role in consumption of kiwiberry(Actinidia arguta).The genetic basis of fruit quality traits in this woody,perennial and dioecious fruit crop remains largely unknown.This study aimed to identify the underlying genetic basis of fruit quality traits in A.arguta,using a single nucleotide polymorphism(SNP)genetic linkage map previously developed in a tetraploid F1 population of‘Ruby-3’בKuiLv-M’.The F1 population was phenotyped over three years(2020–2022)for fruit quality traits,including skin color,flesh color,fruit weight,fruit diameter,total soluble solids,fruit longitudinal diameter and fruit shape index.A total of nine QTLs were detected for five traits,explaining 10%–32%of the trait variation.For fruit color,the support interval of a major QTL on LG9 contained an MYB transcription factor MYB110,which was previously demonstrated to control color regulation in kiwifruit,thus suggesting that the MYB110 is the candidate gene for fruit color in kiwiberry.The linked marker for fruit color was validated in an F1 population and 25 kiwiberry cultivars.In conclusion,the knowledge obtained through the QTL mapping is applicable to improve the efficiency and cost-effectiveness in kiwiberry breeding.展开更多
Actinidia arguta,the most widely distributed Actinidia species and the second cultivated species in the genus,can be distinguished from the currently cultivated Actinidia chinensis on the basis of its small and smooth...Actinidia arguta,the most widely distributed Actinidia species and the second cultivated species in the genus,can be distinguished from the currently cultivated Actinidia chinensis on the basis of its small and smooth fruit,rapid softening,and excellent cold tolerance.Adaptive evolution of tetraploid Actinidia species and the genetic basis of their important agronomic traits are still unclear.Here,we generated a chromosome-scale genome assembly of an autotetraploid male A.arguta accession.The genome assembly was 2.77 Gb in length with a contig N50 of 9.97 Mb and was anchored onto 116 pseudo-chromosomes.Resequencing and clustering of 101 geographically representative accessions showed that they could be divided into two geographic groups,Southern and Northern,which first diverged 12.9 million years ago.A.arguta underwent two prominent expansions and one demographic bottleneck from the midPleistocene climate transition to the late Pleistocene.Population genomics studies using paleoclimate data enabled us to discern the evolution of the species’adaptation to different historical environments.Three genes(AaCEL1,AaPME1,and AaDOF1)related to flesh softening were identified by multi-omics analysis,and their ability to accelerate flesh softening was verified through transient expression assays.A set of genes that characteristically regulate sexual dimorphism located on the sex chromosome(Chr3)or autosomal chromosomes showed biased expression during stamen or carpel development.This chromosome-level assembly of the autotetraploid A.arguta genome and the genes related to important agronomic traits will facilitate future functional genomics research and improvement of A.arguta.展开更多
基金support from the National Natural Science Foundation of China(Grant No.32001995)the Major Science and Technology Project of Henan Province(Grant No.221100110400)+3 种基金the China Agriculture Research System of MOF and MARA(Grant No.CARS-26)the Special Engineering Science and Technology Innovation,CAAS(Grant No.CAAS-ASTIP-2024-ZFRI).Part of this research was funded under the TKI project:“Novel genetic and genomic tools for polyploid crops”(KV 1605-020BO 26.03009004,BO 50002022)and USDA's National Institute of Food and Agriculture(NIFA)Specialty Crop Research Initiative project“Tools for genomics assisted breeding in polyploids:Development of a community resource”(Grant No.2020-51181-32156/SCRI)received a scholarship from China Scholarship Council(Grant No.202303250034).
文摘Fruit quality traits play an important role in consumption of kiwiberry(Actinidia arguta).The genetic basis of fruit quality traits in this woody,perennial and dioecious fruit crop remains largely unknown.This study aimed to identify the underlying genetic basis of fruit quality traits in A.arguta,using a single nucleotide polymorphism(SNP)genetic linkage map previously developed in a tetraploid F1 population of‘Ruby-3’בKuiLv-M’.The F1 population was phenotyped over three years(2020–2022)for fruit quality traits,including skin color,flesh color,fruit weight,fruit diameter,total soluble solids,fruit longitudinal diameter and fruit shape index.A total of nine QTLs were detected for five traits,explaining 10%–32%of the trait variation.For fruit color,the support interval of a major QTL on LG9 contained an MYB transcription factor MYB110,which was previously demonstrated to control color regulation in kiwifruit,thus suggesting that the MYB110 is the candidate gene for fruit color in kiwiberry.The linked marker for fruit color was validated in an F1 population and 25 kiwiberry cultivars.In conclusion,the knowledge obtained through the QTL mapping is applicable to improve the efficiency and cost-effectiveness in kiwiberry breeding.
基金funded by the Chinese National Key Research And Development Program(2019YFD1000202)the Biodiversity Survey,Observation and Assessment Program awarded by the Ministry of Ecology and Environment,The People’s Republic of China(2019HJ2096001006)+2 种基金the International Partnership Program of the Chinese Academy of Sciences(151542KYSB20210004)the Regional Key Projects of Science and Technology Service Network Initiative granted by the Chinese Academy of Sciences(KFJ-STS-QYZD-192)the Natural Science Foundation of China(NSFC)(31372031).
文摘Actinidia arguta,the most widely distributed Actinidia species and the second cultivated species in the genus,can be distinguished from the currently cultivated Actinidia chinensis on the basis of its small and smooth fruit,rapid softening,and excellent cold tolerance.Adaptive evolution of tetraploid Actinidia species and the genetic basis of their important agronomic traits are still unclear.Here,we generated a chromosome-scale genome assembly of an autotetraploid male A.arguta accession.The genome assembly was 2.77 Gb in length with a contig N50 of 9.97 Mb and was anchored onto 116 pseudo-chromosomes.Resequencing and clustering of 101 geographically representative accessions showed that they could be divided into two geographic groups,Southern and Northern,which first diverged 12.9 million years ago.A.arguta underwent two prominent expansions and one demographic bottleneck from the midPleistocene climate transition to the late Pleistocene.Population genomics studies using paleoclimate data enabled us to discern the evolution of the species’adaptation to different historical environments.Three genes(AaCEL1,AaPME1,and AaDOF1)related to flesh softening were identified by multi-omics analysis,and their ability to accelerate flesh softening was verified through transient expression assays.A set of genes that characteristically regulate sexual dimorphism located on the sex chromosome(Chr3)or autosomal chromosomes showed biased expression during stamen or carpel development.This chromosome-level assembly of the autotetraploid A.arguta genome and the genes related to important agronomic traits will facilitate future functional genomics research and improvement of A.arguta.
