AIM To avoid desensitization protocols and ABO incompatible kidney transplantation(KT) due to high costs and increased risk of infections from intense immunosuppression.METHODS We present institutional ethical review ...AIM To avoid desensitization protocols and ABO incompatible kidney transplantation(KT) due to high costs and increased risk of infections from intense immunosuppression.METHODS We present institutional ethical review board- approved study of single center 6-way kidney exchange transplantation. The participants comprised ABO incompatibility(n = 1); positive cross-match and/or presence of donor specific antibody(n = 5). The average time required from registration in kidney paired donation(KPD) registry to find suitable donors was 45 d and time required to perform transplants after legal permission was 2 mo. RESULTS Graft and patient survival were 100%, and 100%, respectively. One patient had biopsy-proven acute borderline T cell rejection(Banff update 2013, type 3). Mean serum creatinine was 0.8 mg/dL at 9 mo followup. The waiting time in KPD was short as compared to deceased donor KT. CONCLUSION We report first non-simultaneous, single center, 6-way kidney exchange transplantation from India. Our experience will encourage other centers in India to undertake this practice.展开更多
The method of warming the kidney and strengthening the spleen is widely used in the treatment of ulcerative colitis (UC) with significant curative effect.This paper reviews the relevant literature in recent years.
Objective To investigate the risk factors and prognosis of mitral regurgitation(MR)in patients with chronic kidney disease(CKD).Methods Clinical data were collected from CKD patients who were hospitalized at the Depar...Objective To investigate the risk factors and prognosis of mitral regurgitation(MR)in patients with chronic kidney disease(CKD).Methods Clinical data were collected from CKD patients who were hospitalized at the Department of Nephrology,Xuanwu Hospital,Capital Medical University,from January 1,2018,to December 31,2019,and underwent echocardiography.Patients were followed up until November 1,2021,with theendpointtbeingall-causemortality.Logistic regression was used to analyze the risk factors of MR in CKD patientsK.aplan-Meier survival analysis was performed to plot the survival curve,with the Log-rank test comparing the survival rate.Multivariate Cox regression analysis was used to identify the risk factors of death in CKD patients.Results It was a retrospective single-center study.A total of 555 CKD patients were included,with 262 patients developing MR,of whom 212 patients had mild MR(80.9%),44 patients had modreate MR(16.8%),6 patients had severe MR(2.3%).The prevalence of MR among patients with CKD stages 1 to 5 was 21.9%,33.0%,45.9%,51.9%,and 64.6%,and the prevalence of moderate to severe MR was 0,5.6%,7.4%,10.1%,and 15.9%.Multivariate logistic regression analysis revealed that male sex(0R=1.579,95%CI 1.008-2.476,P=0.046),presence of chronic heart disease(OR=2.263,95%CI 1.398-3.662,P=0.001),CKD stage 4-5(with CKD stage 1-3 as reference,OR=1.744,95%CI 1.007-3.019,P=0.047),and decreasing hemoglobin levels(0R=0.985,95%CI 0.975-0.996,P=0.006)were the associated factors for MR in CKD patients.Kaplan-Meier survival analysis indicated higher all-cause mortality(Log-rank,X^(2)=8.094,P=0.004)in the MR groupp compared to the non-MRR group.Multivariate Cox xregressionanalysisshowedthat increasing age(HR=1.072,95%CI 1.042-1.104,P<0.001),elevating blood phosphorus(HR=2.782,95%CI 1.122-6.895,P=0.027),MR(HR=1.962,95%CI 1.002-3.839,P=0.049)and decreasing albumin(HR=0.927,95%CI 0.886-0.970,P=0.001)were independent associated factors for all-cause death in CKD patients.Conclusion The overall prevalence of MR and the prevalence of moderate to severe MR are increasing with the progression of CKD.Male sex,presence of chronic heart disease,CKD stage 4-5 and decreasing hemoglobin levels are risk factors for MR in CKD patients.MR is an independent risk factor for all-cause mortality in CKD patients.展开更多
Objective To explore the incidence and risk factors of severe bleeding after percutaneous renal biopsy(PRB)in patients with advanced chronic kidney disease(CKD).Methods The study was a retrospective cohort analysis.Th...Objective To explore the incidence and risk factors of severe bleeding after percutaneous renal biopsy(PRB)in patients with advanced chronic kidney disease(CKD).Methods The study was a retrospective cohort analysis.The data were collected from patients with advanced CKD who were hospitalized in the Department of Nephrology,Nanfang Hospital,Southern Medical University and underwent PRB between January 2010 and December 2020.Severe bleeding after PRB was defined by any of thefollowingcriteria:a postoperativeehemoglobin decrease of≥20 g/L within 48 hours,a maximum diameter of perirenal hematoma≥5 cm postoperatively,or the need for posterior pituitary hormone,blood transfusion,or renal vascular intervention post-surgery.The occurrence of severe bleeding following PRB served as the primary endpoint for this study.Logistic regression model was used to analyze the risk factors associated with severebleeding in patients with advanced1CKD undergoing PRB.Results A total of 895 patients aged(46.1±14.1)years were encompassed in the study.Among them,60.1%(538/895)were male,15.9%(142/895)were afflicted with diabetes,and 57.9%(518/895)suffered from hypertension.The estimated glomerular filtration rate(eGFR)was(40.1±13.2)ml min=1.(1.73 m²)-1,and the 24-hour urine protein excretion was 2.5(1.1,4.9)g.After PRB,22.9%(205/895)of the patients encountered severe bleeding,including 30 patients(14.6%)who received postoperative somatostatin,10 patients(4.9%)who underwent postoperative blood transfusion,1 patient(0.5%)who underwent postoperative renal vascular intervention for hemostasis,and no fatalities occurred.Compared to the non-severe bleeding group,patients in the severe bleeding group after PRB exhibited a higher proportion of hypertension[64.4%(132/205)vs.55.9%(386/690),X=4.627,P=0.031].Additionally,preoperative serum creatinine levels and mean arterial pressure were significantly elevated[(193.9±106.6)μmol/L vs.(180.8±102.6)μmol/L,t=-2.559,P=0.011;(95.8±10.9)mmHg vs.(93.9±11.0)mmHg,t=-2.134,P=0.033].Furthermore,platelet counts were lower in the severe bleeding group[(227.5±70.3)×10/Lvs.(247.5±74.8)×10/L,t=-3.788,P<0.001].No statistically significant differences were observed between the two groups regarding age,gender distribution,prevalence of diabetes mellitus,as well as preoperative serum albumin level,hemoglobin concentration,other coagulation function indicators and pathological histological type(all P>0.05).Multivariate logistic regression analysis indicated that body mass index(OR=0.936,95%CI 0.891-0.984,P=0.010),eGFR(0R=0.985,95%CI 0.971-0.999,P=0.034),serum albumin level(0R=1.041,95%CI 1.011-1.072,P=0.007),24 hours urinary protein excretion(0R=1.092,95%CI 1.030-1.158,P=0.003),and platelet count(OR=0.996,95%CI 0.994-0.999,P=0.002)were independently associated with the severe bleeding following PRB in patients with advanced CKD.In the PRB cohort analyzed,the six most prevalent renal histological types were as follows:IgA nephropathy(46.3%,414/895),membranous nephropathy(11.1%,99/895),focal segmental glomerulosclerosis(8.5%,76/895),diabetic nephropathy(7.6%,68/895),sclerotic kidney disease(6.9%,62/895),and vascular sclerosis of the kidneys(4.9%,44/895).Conclusion Patients with advanced CKD exhibit a heightened risk of severe bleeding following PRB,estimated at approximately 22.9%.Independent risk factors for the occurrence of severe bleeding complications in these patients include low body mass index,reduced eGFR,decreased platelet count,elevated serum albumin,and increased urinary protein level.展开更多
A clinical case of Professor ZHAO Jun's intervention in children's height after menstrual onsets is introduced in this paper,and she believes that regulating spleen and kidney should be paid attention to child...A clinical case of Professor ZHAO Jun's intervention in children's height after menstrual onsets is introduced in this paper,and she believes that regulating spleen and kidney should be paid attention to children in the middle and late stages of puberty.In clinical practice,Pericarpium Citri Reticulatae,Rhizoma Pinelliae and Poria in Erchen Decoction(二陈汤)are used to remove dampness and resolve phlegm,as well as to regulate Qi(气)and invigorate spleen.Fructus Ligustri Lucidi and Herba Ecliptae in Erzhi Pills(二至丸)are applied to invigorate liver and kidney,as well as to regulate Thoroughfare and Conception Channels,so as to achieve the effect of Yin(阴)and Yang(阳)coordination.It has significant efficacy.展开更多
Objective:To probe into the mechanism of the Chinese herbs with functions of reinforcing kidney and supplementing qi for preventing knee osteoarthritis of the rabbit. Methods: Totally 72 healthy Japan long-ear white r...Objective:To probe into the mechanism of the Chinese herbs with functions of reinforcing kidney and supplementing qi for preventing knee osteoarthritis of the rabbit. Methods: Totally 72 healthy Japan long-ear white rabbits, aged 4 months, were randomly divided into 6 groups, blank group (A), model group (B), high dose Chinese herb group (C), middle dose Chinese herb group (D), small dose Chinese herb group (E), aminoglucose hydrochloride capsule control group (F), 12 rabbits in each group. All the rabbits in the groups, except the group A, were fixed with plaster cast for six weeks to establish rabbit knee osteoarthritis. At the same time of modeling, the different doses of Juanbi Capsules and aminoglucose hydrochloride capsule were administrated intragastrically in the group C, D, E, F, respectively, for 4 weeks, for preventive treatment. In the group B, the rabbit was administrated intragastrically with equal volume of normal saline to the medication groups, twice each day, in the morning and the evening, and in the group A, nothing was administrated. After modeling for 6 weeks, the joint fluid was taken and TNF-α, IL-1 and IL-6 contents were detected with ELISA method, and the articular cartilage was taken for macroscopic and microscopic examinations. Results: In all the preventive treatment groups, the articular cartilage color changed to varying degrees with formation of osteophyte and bone cyst, superficial erosion on the chondral articular surface, and the cartilage defect reached to the mid layer in a part of specimens with cartilage exfoliation, but which in the extent were significantly lower than those in the model group. There were significant differences between the group A and B in TNF-α , IL-1 and IL-6 contents in the joint fluid (P<0.05), indicating that the modeling is successful; and there were significant differences as group B compared with the group C,D, E, F, showing that TNF-α , IL-1 and IL-6 contents are decreased in all the medication groups; and significant differences between group C, D, E suggests that the increase of Chinese herb doses strengthened the effect of reducing TNF-α , IL-1 and IL-6 contents in joint fluid. Conclusion: The Juanbi Capsule prevents osteoarthritis possibly through decreasing serum TNF-α, IL-1 and IL-6 contents.展开更多
Erhuang capsule, a typical formula based on traditional Chinese medicine theory, is widely used to ameliorate multiple sclerosis, inflammation and side effects of glucocortJcoid treatment. Oligodendrocyte precursor ce...Erhuang capsule, a typical formula based on traditional Chinese medicine theory, is widely used to ameliorate multiple sclerosis, inflammation and side effects of glucocortJcoid treatment. Oligodendrocyte precursor cells are neural stem cells that are important for myelin repair and regeneration. In the present study, Erhuang capsule effectively improved clinical symptoms and neurological function scores, reduced mortality and promoted recovery of neurological functions of mice with experimental autoimmune encephalomyelitis. The mechanism of action involved significant increases in oligodendrocyte precursor cell proliferation in specific regions of the brain and spinal cord, increased oligodendrocyte lineage gene 2 expression and enhanced oligodendrocyte precursor cell differentiation.展开更多
Background and aim Metabolic dysfunction-associated fatty liver disease(MAFLD)is an alternative description and classification of non-alcoholic fatty liver disease(NAFLD)that may have better utility than NAFLD in clin...Background and aim Metabolic dysfunction-associated fatty liver disease(MAFLD)is an alternative description and classification of non-alcoholic fatty liver disease(NAFLD)that may have better utility than NAFLD in clinical practice.We performed a meta-analysis to quantify the magnitude of the association between MAFLD and risk of both prevalent and incident chronic kidney disease(CKD).Methods We systematically searched PubMed,Medline(OVID),Embase(OVID),Web of Science and Cochrane Library from database inception until 29 May 2022.We included observational studies examining the association between MAFLD and risk of CKD,defined by estimated glomerular filtration rate≤60 mL/min/1.73 m2 or presence of abnormal albuminuria.Meta-analysis was performed using random-effects models to obtain summary HRs or ORs with 95%CIs.Results Seventeen observational studies with aggregate data on 845753 participants were included in meta-analysis.In the 7 cohort studies,the pooled random-effects HR for incident CKD in patients with MAFLD was 1.29(95%CI 1.17 to 1.41,I2=87.0%).In the 10 cross-sectional studies,the pooled random-effects OR for prevalent CKD in patients with MAFLD was 1.35(95%CI 1.11 to 1.64,I2=92.6%).Conclusion MAFLD is significantly associated with an increased prevalence and incidence of CKD.展开更多
Objective:To investigate the influence of kidney-tonifying and blood-generating method on platelet(PLT)growth in the treatment of aplastic anemia(AA)and analyze the characteristics of the clinical factors affecting PL...Objective:To investigate the influence of kidney-tonifying and blood-generating method on platelet(PLT)growth in the treatment of aplastic anemia(AA)and analyze the characteristics of the clinical factors affecting PLT growth.We enrolled patients treated from September 2018to March 2021 in the China Academy of Traditional Chinese Medicine(TCM)Xiyuan Hospital,China Academy of TCM hospital gate 19sub-center.We enrolled 128 AA patients,for whom the inclusion criteria were:treatment is given priority to with kidney and heart's-blood method,on the basis of western medicine treatment for kidney fill party avoid Fried instant particles,stay for 3 months for a period of treatment,taking two consecutive period of treatment,changes in the PLT were observed,and the clinical characteristics of AA patients,such as PLT growth and recovery from baseline value,were analyzed.Materials and Methods:A total of 128 AA patients from 19 branches of Xiyuan Hospital,China Academy of Chinese Medical Sciences and Guang'anmen Hospital,China Academy of Chinese Medical Sciences treated from September 2018 to March 2021 were enrolled.The treatment was based on the kidney-tonifying and blood-generating method and lasted for 3 months as a course of treatment.The changes in the number of PLT in patients were observed,and the clinical characteristics of AA affecting PLT growth and recovery from baseline value were analyzed.Results:After 6 months of treatment following the kidney-tonifying and blood-generating method,PLT increased from the baseline values in 75 cases,including 21 cases of AA with normal PLT recovery and 54 cases of AA without normal PLT recovery.There were 53 cases of AA in whom PLT did not increase from the baseline.Analysis of the general data showed that patients with lower Karnofsky Performance Status(KPS)score were more likely to experience PLT growth or even return to normal after therapy(P<0.05).The results of routine blood analysis showed that when hemoglobin(HGB)was≥60 g/L and the reticulocyte(RET)proportion was≥0.3%before treatment,PLTs were more likely to increase,even returning to normal in some patients(P<0.05).Analysis of bone puncture results before treatment showed that when the proliferation of nucleated cells was low or extremely low in bone puncture examination,with the proportion of granulocytes<40%,and the proportion of erythroid cells<20%,the treatment method was more effective at increasing PLT count(P<0.05).Analysis of the chromosome karyotype results before treatment showed that when the chromosome karyotype was normal,PLTs were more likely to increase or even return to normal after treatment(P<0.05).Analysis of T-lymphocyte subsets before treatment showed that when CD3+human leukocyte antigen(HLA)-DR+proportion was≥30%,PLTs were more likely to increase or even return to normal(P<0.05).Conclusions:AA patients with a low KPS score,higher HGB≥60 g/L,RET proportion≥0.3%,low or extremely low proliferation of nucleated cells,granulocytes proportion<40%,erythroid proportion<20%,normal chromosome karyotype,CD3+HLA-DR+≥30%,should be treated with a kidney-tonifying and blood-generating method.Such patients are more likely to show PLT growth and the values may even return to normal.展开更多
文摘AIM To avoid desensitization protocols and ABO incompatible kidney transplantation(KT) due to high costs and increased risk of infections from intense immunosuppression.METHODS We present institutional ethical review board- approved study of single center 6-way kidney exchange transplantation. The participants comprised ABO incompatibility(n = 1); positive cross-match and/or presence of donor specific antibody(n = 5). The average time required from registration in kidney paired donation(KPD) registry to find suitable donors was 45 d and time required to perform transplants after legal permission was 2 mo. RESULTS Graft and patient survival were 100%, and 100%, respectively. One patient had biopsy-proven acute borderline T cell rejection(Banff update 2013, type 3). Mean serum creatinine was 0.8 mg/dL at 9 mo followup. The waiting time in KPD was short as compared to deceased donor KT. CONCLUSION We report first non-simultaneous, single center, 6-way kidney exchange transplantation from India. Our experience will encourage other centers in India to undertake this practice.
文摘The method of warming the kidney and strengthening the spleen is widely used in the treatment of ulcerative colitis (UC) with significant curative effect.This paper reviews the relevant literature in recent years.
文摘Objective To investigate the risk factors and prognosis of mitral regurgitation(MR)in patients with chronic kidney disease(CKD).Methods Clinical data were collected from CKD patients who were hospitalized at the Department of Nephrology,Xuanwu Hospital,Capital Medical University,from January 1,2018,to December 31,2019,and underwent echocardiography.Patients were followed up until November 1,2021,with theendpointtbeingall-causemortality.Logistic regression was used to analyze the risk factors of MR in CKD patientsK.aplan-Meier survival analysis was performed to plot the survival curve,with the Log-rank test comparing the survival rate.Multivariate Cox regression analysis was used to identify the risk factors of death in CKD patients.Results It was a retrospective single-center study.A total of 555 CKD patients were included,with 262 patients developing MR,of whom 212 patients had mild MR(80.9%),44 patients had modreate MR(16.8%),6 patients had severe MR(2.3%).The prevalence of MR among patients with CKD stages 1 to 5 was 21.9%,33.0%,45.9%,51.9%,and 64.6%,and the prevalence of moderate to severe MR was 0,5.6%,7.4%,10.1%,and 15.9%.Multivariate logistic regression analysis revealed that male sex(0R=1.579,95%CI 1.008-2.476,P=0.046),presence of chronic heart disease(OR=2.263,95%CI 1.398-3.662,P=0.001),CKD stage 4-5(with CKD stage 1-3 as reference,OR=1.744,95%CI 1.007-3.019,P=0.047),and decreasing hemoglobin levels(0R=0.985,95%CI 0.975-0.996,P=0.006)were the associated factors for MR in CKD patients.Kaplan-Meier survival analysis indicated higher all-cause mortality(Log-rank,X^(2)=8.094,P=0.004)in the MR groupp compared to the non-MRR group.Multivariate Cox xregressionanalysisshowedthat increasing age(HR=1.072,95%CI 1.042-1.104,P<0.001),elevating blood phosphorus(HR=2.782,95%CI 1.122-6.895,P=0.027),MR(HR=1.962,95%CI 1.002-3.839,P=0.049)and decreasing albumin(HR=0.927,95%CI 0.886-0.970,P=0.001)were independent associated factors for all-cause death in CKD patients.Conclusion The overall prevalence of MR and the prevalence of moderate to severe MR are increasing with the progression of CKD.Male sex,presence of chronic heart disease,CKD stage 4-5 and decreasing hemoglobin levels are risk factors for MR in CKD patients.MR is an independent risk factor for all-cause mortality in CKD patients.
文摘Objective To explore the incidence and risk factors of severe bleeding after percutaneous renal biopsy(PRB)in patients with advanced chronic kidney disease(CKD).Methods The study was a retrospective cohort analysis.The data were collected from patients with advanced CKD who were hospitalized in the Department of Nephrology,Nanfang Hospital,Southern Medical University and underwent PRB between January 2010 and December 2020.Severe bleeding after PRB was defined by any of thefollowingcriteria:a postoperativeehemoglobin decrease of≥20 g/L within 48 hours,a maximum diameter of perirenal hematoma≥5 cm postoperatively,or the need for posterior pituitary hormone,blood transfusion,or renal vascular intervention post-surgery.The occurrence of severe bleeding following PRB served as the primary endpoint for this study.Logistic regression model was used to analyze the risk factors associated with severebleeding in patients with advanced1CKD undergoing PRB.Results A total of 895 patients aged(46.1±14.1)years were encompassed in the study.Among them,60.1%(538/895)were male,15.9%(142/895)were afflicted with diabetes,and 57.9%(518/895)suffered from hypertension.The estimated glomerular filtration rate(eGFR)was(40.1±13.2)ml min=1.(1.73 m²)-1,and the 24-hour urine protein excretion was 2.5(1.1,4.9)g.After PRB,22.9%(205/895)of the patients encountered severe bleeding,including 30 patients(14.6%)who received postoperative somatostatin,10 patients(4.9%)who underwent postoperative blood transfusion,1 patient(0.5%)who underwent postoperative renal vascular intervention for hemostasis,and no fatalities occurred.Compared to the non-severe bleeding group,patients in the severe bleeding group after PRB exhibited a higher proportion of hypertension[64.4%(132/205)vs.55.9%(386/690),X=4.627,P=0.031].Additionally,preoperative serum creatinine levels and mean arterial pressure were significantly elevated[(193.9±106.6)μmol/L vs.(180.8±102.6)μmol/L,t=-2.559,P=0.011;(95.8±10.9)mmHg vs.(93.9±11.0)mmHg,t=-2.134,P=0.033].Furthermore,platelet counts were lower in the severe bleeding group[(227.5±70.3)×10/Lvs.(247.5±74.8)×10/L,t=-3.788,P<0.001].No statistically significant differences were observed between the two groups regarding age,gender distribution,prevalence of diabetes mellitus,as well as preoperative serum albumin level,hemoglobin concentration,other coagulation function indicators and pathological histological type(all P>0.05).Multivariate logistic regression analysis indicated that body mass index(OR=0.936,95%CI 0.891-0.984,P=0.010),eGFR(0R=0.985,95%CI 0.971-0.999,P=0.034),serum albumin level(0R=1.041,95%CI 1.011-1.072,P=0.007),24 hours urinary protein excretion(0R=1.092,95%CI 1.030-1.158,P=0.003),and platelet count(OR=0.996,95%CI 0.994-0.999,P=0.002)were independently associated with the severe bleeding following PRB in patients with advanced CKD.In the PRB cohort analyzed,the six most prevalent renal histological types were as follows:IgA nephropathy(46.3%,414/895),membranous nephropathy(11.1%,99/895),focal segmental glomerulosclerosis(8.5%,76/895),diabetic nephropathy(7.6%,68/895),sclerotic kidney disease(6.9%,62/895),and vascular sclerosis of the kidneys(4.9%,44/895).Conclusion Patients with advanced CKD exhibit a heightened risk of severe bleeding following PRB,estimated at approximately 22.9%.Independent risk factors for the occurrence of severe bleeding complications in these patients include low body mass index,reduced eGFR,decreased platelet count,elevated serum albumin,and increased urinary protein level.
基金Projects of Shanghai Municipal Health Commission (Overseas, GDEJ201704)。
文摘A clinical case of Professor ZHAO Jun's intervention in children's height after menstrual onsets is introduced in this paper,and she believes that regulating spleen and kidney should be paid attention to children in the middle and late stages of puberty.In clinical practice,Pericarpium Citri Reticulatae,Rhizoma Pinelliae and Poria in Erchen Decoction(二陈汤)are used to remove dampness and resolve phlegm,as well as to regulate Qi(气)and invigorate spleen.Fructus Ligustri Lucidi and Herba Ecliptae in Erzhi Pills(二至丸)are applied to invigorate liver and kidney,as well as to regulate Thoroughfare and Conception Channels,so as to achieve the effect of Yin(阴)and Yang(阳)coordination.It has significant efficacy.
基金supported by grants from Special Scientific Research Plan of the Education Department of Shaanxi Province (09JK411)Special Foundation of Key Discipline of the Education Department of Shaanxi Province and Special Foundation of Key Specialty Construction of State TCM Administration of China.
文摘Objective:To probe into the mechanism of the Chinese herbs with functions of reinforcing kidney and supplementing qi for preventing knee osteoarthritis of the rabbit. Methods: Totally 72 healthy Japan long-ear white rabbits, aged 4 months, were randomly divided into 6 groups, blank group (A), model group (B), high dose Chinese herb group (C), middle dose Chinese herb group (D), small dose Chinese herb group (E), aminoglucose hydrochloride capsule control group (F), 12 rabbits in each group. All the rabbits in the groups, except the group A, were fixed with plaster cast for six weeks to establish rabbit knee osteoarthritis. At the same time of modeling, the different doses of Juanbi Capsules and aminoglucose hydrochloride capsule were administrated intragastrically in the group C, D, E, F, respectively, for 4 weeks, for preventive treatment. In the group B, the rabbit was administrated intragastrically with equal volume of normal saline to the medication groups, twice each day, in the morning and the evening, and in the group A, nothing was administrated. After modeling for 6 weeks, the joint fluid was taken and TNF-α, IL-1 and IL-6 contents were detected with ELISA method, and the articular cartilage was taken for macroscopic and microscopic examinations. Results: In all the preventive treatment groups, the articular cartilage color changed to varying degrees with formation of osteophyte and bone cyst, superficial erosion on the chondral articular surface, and the cartilage defect reached to the mid layer in a part of specimens with cartilage exfoliation, but which in the extent were significantly lower than those in the model group. There were significant differences between the group A and B in TNF-α , IL-1 and IL-6 contents in the joint fluid (P<0.05), indicating that the modeling is successful; and there were significant differences as group B compared with the group C,D, E, F, showing that TNF-α , IL-1 and IL-6 contents are decreased in all the medication groups; and significant differences between group C, D, E suggests that the increase of Chinese herb doses strengthened the effect of reducing TNF-α , IL-1 and IL-6 contents in joint fluid. Conclusion: The Juanbi Capsule prevents osteoarthritis possibly through decreasing serum TNF-α, IL-1 and IL-6 contents.
基金supported by the Key Combination Program of Capital Medical Development Foundation,No.2005-SF-I-001the Natural Science Foundation of Beijing,No.7102051,7092014+1 种基金the Beijing Science and Technology Development Foundation of Traditional Chinese Medicine,No.JJ2009-27the National Natural Science Foundation of China,No. 81072765,30873230
文摘Erhuang capsule, a typical formula based on traditional Chinese medicine theory, is widely used to ameliorate multiple sclerosis, inflammation and side effects of glucocortJcoid treatment. Oligodendrocyte precursor cells are neural stem cells that are important for myelin repair and regeneration. In the present study, Erhuang capsule effectively improved clinical symptoms and neurological function scores, reduced mortality and promoted recovery of neurological functions of mice with experimental autoimmune encephalomyelitis. The mechanism of action involved significant increases in oligodendrocyte precursor cell proliferation in specific regions of the brain and spinal cord, increased oligodendrocyte lineage gene 2 expression and enhanced oligodendrocyte precursor cell differentiation.
基金supported by grants from the National Natural Science Foundation of China(82070588 and 82000690)High Level Creative Talents from Department of Public Health in Zhejiang Province(S2032102600032)+1 种基金Project of New Century 551 Talent Nurturing in Wenzhou.GT is supported in part by grants from the University School of Medicine of Verona,Verona,ItalyCDB is supported in part by the Southampton NIHR Biomedical Research Centre(IS-BRC-20004),UK.
文摘Background and aim Metabolic dysfunction-associated fatty liver disease(MAFLD)is an alternative description and classification of non-alcoholic fatty liver disease(NAFLD)that may have better utility than NAFLD in clinical practice.We performed a meta-analysis to quantify the magnitude of the association between MAFLD and risk of both prevalent and incident chronic kidney disease(CKD).Methods We systematically searched PubMed,Medline(OVID),Embase(OVID),Web of Science and Cochrane Library from database inception until 29 May 2022.We included observational studies examining the association between MAFLD and risk of CKD,defined by estimated glomerular filtration rate≤60 mL/min/1.73 m2 or presence of abnormal albuminuria.Meta-analysis was performed using random-effects models to obtain summary HRs or ORs with 95%CIs.Results Seventeen observational studies with aggregate data on 845753 participants were included in meta-analysis.In the 7 cohort studies,the pooled random-effects HR for incident CKD in patients with MAFLD was 1.29(95%CI 1.17 to 1.41,I2=87.0%).In the 10 cross-sectional studies,the pooled random-effects OR for prevalent CKD in patients with MAFLD was 1.35(95%CI 1.11 to 1.64,I2=92.6%).Conclusion MAFLD is significantly associated with an increased prevalence and incidence of CKD.
文摘Objective:To investigate the influence of kidney-tonifying and blood-generating method on platelet(PLT)growth in the treatment of aplastic anemia(AA)and analyze the characteristics of the clinical factors affecting PLT growth.We enrolled patients treated from September 2018to March 2021 in the China Academy of Traditional Chinese Medicine(TCM)Xiyuan Hospital,China Academy of TCM hospital gate 19sub-center.We enrolled 128 AA patients,for whom the inclusion criteria were:treatment is given priority to with kidney and heart's-blood method,on the basis of western medicine treatment for kidney fill party avoid Fried instant particles,stay for 3 months for a period of treatment,taking two consecutive period of treatment,changes in the PLT were observed,and the clinical characteristics of AA patients,such as PLT growth and recovery from baseline value,were analyzed.Materials and Methods:A total of 128 AA patients from 19 branches of Xiyuan Hospital,China Academy of Chinese Medical Sciences and Guang'anmen Hospital,China Academy of Chinese Medical Sciences treated from September 2018 to March 2021 were enrolled.The treatment was based on the kidney-tonifying and blood-generating method and lasted for 3 months as a course of treatment.The changes in the number of PLT in patients were observed,and the clinical characteristics of AA affecting PLT growth and recovery from baseline value were analyzed.Results:After 6 months of treatment following the kidney-tonifying and blood-generating method,PLT increased from the baseline values in 75 cases,including 21 cases of AA with normal PLT recovery and 54 cases of AA without normal PLT recovery.There were 53 cases of AA in whom PLT did not increase from the baseline.Analysis of the general data showed that patients with lower Karnofsky Performance Status(KPS)score were more likely to experience PLT growth or even return to normal after therapy(P<0.05).The results of routine blood analysis showed that when hemoglobin(HGB)was≥60 g/L and the reticulocyte(RET)proportion was≥0.3%before treatment,PLTs were more likely to increase,even returning to normal in some patients(P<0.05).Analysis of bone puncture results before treatment showed that when the proliferation of nucleated cells was low or extremely low in bone puncture examination,with the proportion of granulocytes<40%,and the proportion of erythroid cells<20%,the treatment method was more effective at increasing PLT count(P<0.05).Analysis of the chromosome karyotype results before treatment showed that when the chromosome karyotype was normal,PLTs were more likely to increase or even return to normal after treatment(P<0.05).Analysis of T-lymphocyte subsets before treatment showed that when CD3+human leukocyte antigen(HLA)-DR+proportion was≥30%,PLTs were more likely to increase or even return to normal(P<0.05).Conclusions:AA patients with a low KPS score,higher HGB≥60 g/L,RET proportion≥0.3%,low or extremely low proliferation of nucleated cells,granulocytes proportion<40%,erythroid proportion<20%,normal chromosome karyotype,CD3+HLA-DR+≥30%,should be treated with a kidney-tonifying and blood-generating method.Such patients are more likely to show PLT growth and the values may even return to normal.
