为深入挖掘玉米籽粒容重的相关优势基因,以348份包含普通玉米、甜玉米、糯玉米、爆裂玉米等遗传多样性丰富的玉米自交系为关联群体进行全基因组关联分析(Genome-Wide Association Study,GWAS),分别对2022年浙江东阳、2022和2023年海南...为深入挖掘玉米籽粒容重的相关优势基因,以348份包含普通玉米、甜玉米、糯玉米、爆裂玉米等遗传多样性丰富的玉米自交系为关联群体进行全基因组关联分析(Genome-Wide Association Study,GWAS),分别对2022年浙江东阳、2022和2023年海南乐东收获的自交系种子进行容重测定和全基因组关联分析,分析不同品种玉米籽粒容重性状差异,挖掘容重相关位点和控制容重的候选基因。结果表明:浙江东阳和海南乐东的玉米籽粒容重性状指标的频率分布呈单峰型曲线,符合正态分布。利用SNP标记全基因组关联分析,以阈值P>8.7筛选出与玉米籽粒容重性状显著相关的SNP位点,2022年浙江东阳地区筛选出SNP位点146个,2022年海南乐东地区筛选出261个,2023年海南乐东地区筛选出705个,重叠SNP位点共67个,最终在相关SNP位点附近筛选出候选基因14个。其中,同时在浙江东阳(2022年)、海南乐东(2022和2023年)不同环境下均检测到的SNP S3_219692500位于Zm00001d044129(Shrunken2)基因区域,该基因为玉米甜度控制基因,即该基因突变后产生超甜玉米,籽粒皱缩,玉米容重下降。展开更多
Four released durum wheat varieties, two medium tall (Arsi-Robe and Kilinto) and two recently released semidwarfs (Ude and Yerer) were evaluated under five different N rates (0, 60, 120, 180 and 240 kg·ha-1) in 2...Four released durum wheat varieties, two medium tall (Arsi-Robe and Kilinto) and two recently released semidwarfs (Ude and Yerer) were evaluated under five different N rates (0, 60, 120, 180 and 240 kg·ha-1) in 2005/06 growing season at Akaki and Debre Zeit, central Ethiopia. The quality responses of durum wheat varieties to different N-fertilizer rates and the N responses between tall and short durum wheat varieties were assessed. Protein and gluten content, vitreous kernel count, thousand kernel weight, test weight and lodging were considered. Vitreous kernel count, protein and gluten content were higher at Debre Zeit than at Akaki. Unlike Debre Zeit, there were significant relationships between response variables and fertilizer levels at Akaki. Lodging slightly affected the medium tall varieties at Debre Zeit whereas it was not a problem at Akaki. At Akaki, varieties response to each additional N rate was entirely below the quality standard set by ICC, 2000 except for thousandkernel weight. Therefore, it is unwise to promote durum wheat scaling up/out program for quality production at Akaki.展开更多
文摘为深入挖掘玉米籽粒容重的相关优势基因,以348份包含普通玉米、甜玉米、糯玉米、爆裂玉米等遗传多样性丰富的玉米自交系为关联群体进行全基因组关联分析(Genome-Wide Association Study,GWAS),分别对2022年浙江东阳、2022和2023年海南乐东收获的自交系种子进行容重测定和全基因组关联分析,分析不同品种玉米籽粒容重性状差异,挖掘容重相关位点和控制容重的候选基因。结果表明:浙江东阳和海南乐东的玉米籽粒容重性状指标的频率分布呈单峰型曲线,符合正态分布。利用SNP标记全基因组关联分析,以阈值P>8.7筛选出与玉米籽粒容重性状显著相关的SNP位点,2022年浙江东阳地区筛选出SNP位点146个,2022年海南乐东地区筛选出261个,2023年海南乐东地区筛选出705个,重叠SNP位点共67个,最终在相关SNP位点附近筛选出候选基因14个。其中,同时在浙江东阳(2022年)、海南乐东(2022和2023年)不同环境下均检测到的SNP S3_219692500位于Zm00001d044129(Shrunken2)基因区域,该基因为玉米甜度控制基因,即该基因突变后产生超甜玉米,籽粒皱缩,玉米容重下降。
文摘Four released durum wheat varieties, two medium tall (Arsi-Robe and Kilinto) and two recently released semidwarfs (Ude and Yerer) were evaluated under five different N rates (0, 60, 120, 180 and 240 kg·ha-1) in 2005/06 growing season at Akaki and Debre Zeit, central Ethiopia. The quality responses of durum wheat varieties to different N-fertilizer rates and the N responses between tall and short durum wheat varieties were assessed. Protein and gluten content, vitreous kernel count, thousand kernel weight, test weight and lodging were considered. Vitreous kernel count, protein and gluten content were higher at Debre Zeit than at Akaki. Unlike Debre Zeit, there were significant relationships between response variables and fertilizer levels at Akaki. Lodging slightly affected the medium tall varieties at Debre Zeit whereas it was not a problem at Akaki. At Akaki, varieties response to each additional N rate was entirely below the quality standard set by ICC, 2000 except for thousandkernel weight. Therefore, it is unwise to promote durum wheat scaling up/out program for quality production at Akaki.
