Nutrients play a crucial role in sustaining marine ecosystems and supporting mariculture,especially in seaweed aquaculture.Currently,seaweed farming,such as kelp cultivation,is entirely dependent on the natural supply...Nutrients play a crucial role in sustaining marine ecosystems and supporting mariculture,especially in seaweed aquaculture.Currently,seaweed farming,such as kelp cultivation,is entirely dependent on the natural supply of nutrients.Sanggou Bay in Shandong Peninsula,Yellow Sea,is renowned for its 60-year history of kelp cultivation;however,it is recently facing an increasing demand for nitrogen and phosphorus due to the expansion in aquaculture scale and production.There is no doubt that nutrient addition can enhance and sustain the production,but it is crucial to understand its effect on kelp growth under current nutrients condition and the potential ecological risks.Our in-field nutrient enrichment experiments show that nitrogen and phosphorus additions promoted the kelp biomass during the early growth stages,and have no adverse effects on phytoplankton or seawater nutrient levels throughout the experiment.From a long-term perspective,increasing nutrient supply appears to be an essential strategy for sustaining the aquaculture of kelp.展开更多
Giant kelp Macrocystis pyrifera,an important foundation species with great ecological and economic value,is threatened by climate change.To better understand the impact of climate warming on M.pyrifera,we investigated...Giant kelp Macrocystis pyrifera,an important foundation species with great ecological and economic value,is threatened by climate change.To better understand the impact of climate warming on M.pyrifera,we investigated its global distribution dynamics by an optimized species distribution model(SDM).Results showed that wave height,sea surface temperature,benthic temperature,and benthic phosphate concentration were key factors shaping the distribution of M.pyrifera.In addition to currently known distribution regions,the model revealed potential suitable habitats globally.Under future climate scenarios,the habitat suitability of M.pyrifera would decrease at low latitudes and increase at high latitudes,resulting in a poleward shift of suitable habitats.In the regions currently occupied by M.pyrifera,the high suitable habitats were predicted to shrink,which implies that the existing M.pyrifera would be adversely impacted.These results serve as references for the conservation and utilization of M.pyrifera resource.展开更多
The most important marine coastal ecosystem in the Chilean coast are kelp forests.This review is based on ecological studies regarding different aspects of subtidal kelp ecosystems along the Chilean coast.It highlight...The most important marine coastal ecosystem in the Chilean coast are kelp forests.This review is based on ecological studies regarding different aspects of subtidal kelp ecosystems along the Chilean coast.It highlights the most interesting findings in(1)biology of subtidal kelp in Chile,with particular reference to(2)habitats formed by kelp,and considered the successful examples and promising results in the(3)kelp as an industrial resource(Biotechnological approach of kelps and aquaculture).The impact caused by(4)El Niño-Southern Oscillation is discussed as an important climatic event that could help to forecast the future of the kelp ecosystem.In addition,this literature review outlines the knowledge gaps on subtidal kelp along the Southeast Pacific Coast of Chile,so that research can be strengthened in the future.展开更多
Stable isotope analysis was used to determine the relative dietary importance of kelp-derived detritus to plankton and benthic organisms along a gradient of kelp abundance driven by recovering sea otter populations al...Stable isotope analysis was used to determine the relative dietary importance of kelp-derived detritus to plankton and benthic organisms along a gradient of kelp abundance driven by recovering sea otter populations along the west coast of Vancouver Island (WCVI), Canada. The study used region-specific kelp isotope values (δ<sup>13</sup>C and δ<sup>15</sup>N) and season-specific phytoplankton isotope values to model dietary contributions of kelp-derived detritus (KDD). In general, KDD contributions were moderate to high in most plankton size fractions during the summer and decreased during the winter, particularly in the kelp sparse region. Hypothesized regional and spatial (distance from the coast) differences in kelp detritus contributions to zooplankton w<span style="white-space:normal;"><span style="font-family:;" "="">ere<span style="white-space:normal;"><span style="font-family:;" "=""> not evident. Modeled estimates of the KDD contribution to benthic invertebrates w<span style="white-space:normal;"><span style="font-family:;" "="">ere<span style="white-space:normal;"><span style="font-family:;" "=""> high (>40%) and independent of the organism size, among regions and between seasons, with the exception of Astraea gibberosa in the kelp abundant region. Local oceanography, natural kelp isotope signature variation, and significant overlap between kelps’ and blooming phytoplankton isotope values led to a large uncertainty in the assessed KDD contributions in benthic organisms. These results highlighted the importance of the KDD as a widespread and stable year-round food source in coastal kelp populated regions.展开更多
Several polyculture models of scallop Chlamys farreri and kelp Laminaria japonica currently employedin Sungo Bay and other parts of northern China are described in this paper. Economic benefits of differ-ent polycultu...Several polyculture models of scallop Chlamys farreri and kelp Laminaria japonica currently employedin Sungo Bay and other parts of northern China are described in this paper. Economic benefits of differ-ent polyculture models are analysed based on the growth rate, culture density and market price. In addi-tion, site selection, critical environmental conditions and polyculture problems are discussed in detail.展开更多
Bivalves and seaweeds are important cleaners that are widely used in integrated multi-trophic aquaculture （IMTA） systems. A beneficial relationship between seaweed and bivalve in the seaweed- based IMTA system has b...Bivalves and seaweeds are important cleaners that are widely used in integrated multi-trophic aquaculture （IMTA） systems. A beneficial relationship between seaweed and bivalve in the seaweed- based IMTA system has been confirmed, but the trophic importance of seaweed-derived particulate organic materials to the co-cultured bivalve is still unclear. We evaluated the trophic importance of the kelp Saccharinajaponica to the co-cultured scallop Chlamysfarreri in a typical IMTA farm in Sungo Bay （Weihai, North China）. The dynamics of detritus carbon in the water were monitored during the culturing period. The proportion of kelp-derived organic matter in the diet of the co-cultured scallop was assessed via the stable carbon isotope method. Results showed that the detritus carbon in the water ranged from 75.52 to 265.19 ~tg/L, which was 25.6% to 73.8% of total particulate organic carbon （TPOC） during the study period. The amount of detritus carbon and its proportion in the TPOC changed throughout the culture cycle of the kelp. Stable carbon isotope analysis showed that the cultured scallop obtained 14.1% to 42.8% of its tissue carbon from the kelp, and that the percentages were closely correlated with the proportion of detritus carbon in the water （F=0.993, P=0.003）. Evaluation showed that for 17 000 tons （wet weight） of annual scallop production, the kelp contributed about 139.3 tons of carbon （535.8 tons of dry mass）. This confirms that cultured kelp plays a similar trophic role in IMTA systems as it does in a natural kelp bed. It is a major contributor to the detritus pool and supplies a vital food source to filter-feeding scallops in the IMTA system, especially during winter and early spring when phytoplankton are scarce.展开更多
Integrated Multi-Trophic Aquaculture(IMTA)is an effective method for sustainable aquaculture as species from different trophic levels could reduce negative effects from fed species in the environment.A proper proporti...Integrated Multi-Trophic Aquaculture(IMTA)is an effective method for sustainable aquaculture as species from different trophic levels could reduce negative effects from fed species in the environment.A proper proportion of different trophic species in an IMTA system could improve the aquaculture production and environmental sustainability.At present,research on the proper proportions for farming species is scarce.We investigated the effects of IMTA modes of oyster(Crassostrea gigas)and kelp(Saccharina japonica)in different weight ratios on water quality and carbonate system in a closed enclosure experiment for three days in the Sanggou Bay in Shandong Province,China,in December 2017.Nine collocation modes in oyster:kelp weight ratio were tested showing as 24:3,24:2,24:1,16:3,16:2,16:1,8:3,8:2,and 8:1.The water parameters were determined at 17:00 on Day 1(D1),and 6:00 and 17:00 on Days 2(D2)and 3(D3).As two-way ANOVA showed,all increased parameters(dissolved oxygen(DO),pH,chl a,the carbonate system and pCO2)were significantly related to oyster-kelp modes,and interaction between modes and time were also significant(P<0.05).On the 3th day,the 8:3 mode was the highest in DO,pH,chl a,CO32-(P<0.05),and dissolved inorganic carbon(DIC),HCO3-,CO2,and pCO2 were the lowest(P<0.05).According to previous references and the results of this study,the appropriate oyster:kelp proportion at the beginning of winter is from 8:2 to 8:3.The results of this study may help government to optimize the aquaculture structure of Sanggou Bay.展开更多
Laminaria japonica Aresch as an edible and medicine dual-purpose marine algae,has been gradually accepted by people.Tofu was Chinese traditional food,combining kelp function ingredients with tofu has practical value.T...Laminaria japonica Aresch as an edible and medicine dual-purpose marine algae,has been gradually accepted by people.Tofu was Chinese traditional food,combining kelp function ingredients with tofu has practical value.The extraction-optimization methodology and the sequences of kelp antihypertensive peptide(KAP)were investigated.The sensory,whiteness,water retention ability(WRA)and microstructure of KAP tofu were evaluated.The scanning electron microscope(SEM)results showed KAP addition(20%)significantly improved the tofu texture,with a denser network composed of relatively even pores.Compared with gypsum tofu and glucose-δ-interior fat(GDL)tofu,KAP tofu had significantly higher WRA(94.49±0.49)%and sensory evaluation score value(92.54±0.52),and a significantly lower IC_(50) value at(2.06±0.04)mg/mL(P<0.05).The maximum IC_(50) value(4.14 mg/mL)of kelp enzymatic hydrolysate was observed at enzyme(at an alkaline protease:trypsin ratio of 2:1)concentration 1.5%,temperature 55℃ and time 2 h.The sequences of 8 identified peptides were Lys-Tyr,Phe-Tyr,Gly-Lys-Tyr,Ala-Lys-Tyr,Ser-Lys-Thr-Tyr,Lys-Lys-Phe-Tyr,Lys-Phe-Lys-Tyr and Ala-Lys-Tyr-Ser-Tyr with IC_(50) values of 5.24,4.83,7.94,7.52,20.63,15.33,10.73 and 2.42μmol/L,respectively.These results indicated the potential use of KAP tofu as a valuable resource for development of functional foods.展开更多
An artificial way to prevent and cure the iodine deficiency disorder(IDD) through ameliorating the biogeochemical environment of the regions where iodine is deficient was put forward. In this paper, the concrete meth...An artificial way to prevent and cure the iodine deficiency disorder(IDD) through ameliorating the biogeochemical environment of the regions where iodine is deficient was put forward. In this paper, the concrete method to achieve this way was given and its feasibility is confirmed by test.展开更多
The kelp grouper (Epinephelus bruneus), belonging to one of the largest genera among the subfamily Epinephelinae, is a commercially important fish in Japan. There are limited data about the genomics of this species. T...The kelp grouper (Epinephelus bruneus), belonging to one of the largest genera among the subfamily Epinephelinae, is a commercially important fish in Japan. There are limited data about the genomics of this species. To provide tools for addressing both population genetics studies and gene mapping, dito pentanucleotide simple sequence repeat (SSR) markers were developed using 454 pyrosequencing. Among the 1466 SSR markers developed, 1244 primer sets produced strong PCR products, of which 905 (72.7%) were polymorphic in kelp grouper. Cross-species utility of the 905 polymorphic SSR markers was tested in four additional Epinephelinae species of Hyporthodus septemfasciatus, Plectropomus leopardus, Epinephelus lanceolatus and Epinephelus coioides. Results revealed that, respectively, 401 (44.3%), 136 (15.0%), 434 (49.0%) and 538 (59.4%) SSRs showed specific polymorphic products. Of these, 40 SSR markers (33 di-, 1 tri- and 6 tetra-nucleotides) showed polymorphism in all species tested. Additionally, three AGAT SSR motifs which accounted for 42.9% of the nondi-nucleotide markers were found in the 40 SSR markers. This indicates that the AGAT SSR motif has a high potential as a highly versatile SSR marker in grouper Epinephelinae. The SSR markers developed in this study can be employed to obtain reliable genetic variability estimates for groupers (Epinephelinae).展开更多
Flexible energy-storage devices play a critical role in the development of portable, flexible and wearable electronics. In addition, biological materials including plants or plant-based materials are known for their s...Flexible energy-storage devices play a critical role in the development of portable, flexible and wearable electronics. In addition, biological materials including plants or plant-based materials are known for their safety, biodegradability, biocompatibility, environmental benignancy, and low cost. With respect to these advances, a flexible alkaline zinc-manganese dioxide (Zn-MnO2) battery is fabricated with a kelp-based electrolyte in this study. To the best of our knowledge, pure kelp is utilized as a semi-solid electrolyte for flexible Zn-MnO2 alkaline batteries for the first time, with which the as-assembled battery exhibited a specific capacity of 60 mA·h and could discharge for 120 h. Furthermore, the as-assembled Zn-MnO2 battery can be bent into a ring-shape and power a light-emitting diode screen, showing promising potential for the practical application in the future flexible, portable and biodegradable electronic devices.展开更多
The release process and influencing factors of bioactive iodine of kelp are systemically studied by leaching experiment. The results showed that the bioactive iodine of kelp can be released rapidly and the principal f...The release process and influencing factors of bioactive iodine of kelp are systemically studied by leaching experiment. The results showed that the bioactive iodine of kelp can be released rapidly and the principal form of iodine in lixivium is I -. There is a dynamic process between the release and absorption of iodine. With the increase of leaching water, the gross amounts of released iodine rise. There also exists a transforming process among I -, IO - 3 and organic iodine in lixivium.展开更多
The aim of the study is to examine the influence of the outplanting time on the cultivation of the kelp Laminaria saccharina in the northwestern Spain, close to its southern limit of distribution in the North Atlantic...The aim of the study is to examine the influence of the outplanting time on the cultivation of the kelp Laminaria saccharina in the northwestern Spain, close to its southern limit of distribution in the North Atlantic coast. We compared two L. saccharina cultures outplanted in February and December. We found that the starting time of outplanting affects to the yield and the substantiality value, as well as the growth rate. The yield, the substantiality value and the growth rates of L. saccharina sporophytes obtained for the trial outplanted in December were significantly higher than those in February. These results can be mainly explained by the effect of temperature on the growth of L. saccharina sporophytes during cultivation. On the other hand, our results show that even in the southern limit of distribution of L. saccharina, the cultivation of this species was very profitable in high yield and quality.展开更多
Ecological prevention and control of plant disease is very important in sustainable agriculture.Adjusting soil p H value and fertilizing organic microbial fertilizer are two effective measures in this process.Kelp res...Ecological prevention and control of plant disease is very important in sustainable agriculture.Adjusting soil p H value and fertilizing organic microbial fertilizer are two effective measures in this process.Kelp residue contains a large amount of organic compounds and alkaline metal ions.The bio-control Bacillus amyloliquefaciens strain Hitwh-BA2 was inoculated into kelp residue medium to produce kelp residue microbial fertilizer.Acidic soil and alkaline soil were used to study the effect of kelp residue microbial fertilizer on soil p H and soil suppressive activity.Tip-culture method was used to determine soil leachate suppressive activity,which characterized the soil suppressive activity.Results showed that fertilizing kelp residue microbial fertilizer had increased the soil p H and soil suppressive ability significantly,which was verified by peanut validation experiments as well.Peanut potting experiments proved that fertilizing kelp residue microbial fertilizer not only improved the yield of peanuts obviously,but also reduced the amount of Aspergillus parasiticus 95 in peanut geocarposphere soil significantly.Results also showed that fertilizing kelp residue microbial fertilizer was effective in reducing A.parasiticus 95 infection rate.So the kelp residue microbial fertilizer has good potential application prospect on ecological prevention and control of plant disease.展开更多
This study was carried out explore the mechanism underlying the inhibition of platelet activation by kelp fucoidans in deep venous thrombosis(DVT)mouse.In the control and sham mice,the walls of deep vein were regular ...This study was carried out explore the mechanism underlying the inhibition of platelet activation by kelp fucoidans in deep venous thrombosis(DVT)mouse.In the control and sham mice,the walls of deep vein were regular and smooth with intact intima,myometrium and adventitia.The blood vessel was wrapped with the tissue and there was no thrombosis in the lumen.In the DVT model,the wall was uneven with thicken intima,myometrium and adventitia.After treated with fucoidans LF1 and LF2,the thrombus was dissolved and the blood vessel was recanalized.Compared with the control group,the ROS content,ET-1 and VWF content and the expression of PKC-βand NF-κB in the model were significantly higher(P<0.05);these levels were significantly reduced following treatments with LF2 and LF1.Compared with H_(2)O_(2)treated-HUVECs,combined LF1 and LF2 treatment resulted in significant decrease in the expression of PKC-β,NF-κB,VWF and TM protein(P<0.05).It is clear that LF1 and LF2 reduces DVT-induced ET-1,VWF and TM expressions and production of ROS,thus inhibiting the activation of PKC-β/NF-κB signal pathway and the activation of coagulation system and ultimately reducing the formation of venous thrombus.展开更多
In order to study the development status of kelp industry in Rongcheng in recent years, the centralization degree of kelp industry clusters and the comparative advantage of kelp industry in Rongcheng, Xiapu and Jinpu ...In order to study the development status of kelp industry in Rongcheng in recent years, the centralization degree of kelp industry clusters and the comparative advantage of kelp industry in Rongcheng, Xiapu and Jinpu from 2010 to 2020 were compared and analyzed by indicators such as location quotient index, industrial agglomeration index, coefficient of concentration, scale advantage index, efficiency advantage index, and comprehensive advantage index, and the deep-seated factors affecting the competitiveness of kelp industry in Rongcheng were analyzed. Meanwhile, measures of improving the competitiveness of the kelp industry cluster in Rongcheng were put forward.展开更多
Dried kelp is one of the most important Japanese foods mainly used as base ingredient. Holdfasts, or rhizoids, of kelp are usually discarded, as rocks, shells, ropes and other contaminants are entangled among them. Th...Dried kelp is one of the most important Japanese foods mainly used as base ingredient. Holdfasts, or rhizoids, of kelp are usually discarded, as rocks, shells, ropes and other contaminants are entangled among them. The purpose of the present study was to examine whether the preparation of activated carbon from holdfasts of <em>Saccharina japonica</em>, a popular kelp harvested in Japan, is possible by a chemical activation technique utilizing zinc chloride, and to study the influence of the process parameters, such as carbonization temperature and the composition of the activator. Under the examined experimental conditions, maximum specific surface area of 1.7 × 10<sup>3</sup> m<sup>2</sup>/g, larger than that of a commercial activated carbon, was observed at 600℃ and the zinc chloride composition of 75 mass%.展开更多
At present, the marine environment is faced with extremely serious eutrophication. The kelp＇s ability of taking in nitrogen and phosphorus can be fully performed so that the eutrophication problem can be effectively ...At present, the marine environment is faced with extremely serious eutrophication. The kelp＇s ability of taking in nitrogen and phosphorus can be fully performed so that the eutrophication problem can be effectively treated and solved. Based on the analysis of kelp＇s absorption of nutritive salt from aquaculture sewage, this paper further explores the bioremediation of kelp in aquaculture sea area with eutrophication in the hope of providing valuable reference for the aquaculture industry of BohaiSea.展开更多
基金Supported by the Laoshan Laboratory(No.LSKJ202204005)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA23050502)+2 种基金the State Key Program of National Natural Science Foundation of China(No.42130411)the International Science Partnership Program of the Chinese Academy of Sciences(Nos.121311KYSB20190029,133137KYSB20200002)the Key Deployment Project of Centre for Ocean Mega-Research of Science,Chinese Academy of Sciences(CAS)(No.COMS2019J03)。
文摘Nutrients play a crucial role in sustaining marine ecosystems and supporting mariculture,especially in seaweed aquaculture.Currently,seaweed farming,such as kelp cultivation,is entirely dependent on the natural supply of nutrients.Sanggou Bay in Shandong Peninsula,Yellow Sea,is renowned for its 60-year history of kelp cultivation;however,it is recently facing an increasing demand for nitrogen and phosphorus due to the expansion in aquaculture scale and production.There is no doubt that nutrient addition can enhance and sustain the production,but it is crucial to understand its effect on kelp growth under current nutrients condition and the potential ecological risks.Our in-field nutrient enrichment experiments show that nitrogen and phosphorus additions promoted the kelp biomass during the early growth stages,and have no adverse effects on phytoplankton or seawater nutrient levels throughout the experiment.From a long-term perspective,increasing nutrient supply appears to be an essential strategy for sustaining the aquaculture of kelp.
基金Supported by the National Key Research and Development Program of China(No.2023YFD2400800)the Laoshan Laboratory(Nos.LSKJ202203801,LSKJ202203204)+4 种基金the Natural Science Foundation of Shandong Province(Nos.ZR2023MD127,ZR2021MD075)the Central Public-interest Scientific Institution Basal Research Fund CAFS(Nos.2023TD28,20603022023012)the National Natural Science Foundation of China(No.32373107)the China Agriculture Research System(No.CARS-50)the Taishan Scholars Program。
文摘Giant kelp Macrocystis pyrifera,an important foundation species with great ecological and economic value,is threatened by climate change.To better understand the impact of climate warming on M.pyrifera,we investigated its global distribution dynamics by an optimized species distribution model(SDM).Results showed that wave height,sea surface temperature,benthic temperature,and benthic phosphate concentration were key factors shaping the distribution of M.pyrifera.In addition to currently known distribution regions,the model revealed potential suitable habitats globally.Under future climate scenarios,the habitat suitability of M.pyrifera would decrease at low latitudes and increase at high latitudes,resulting in a poleward shift of suitable habitats.In the regions currently occupied by M.pyrifera,the high suitable habitats were predicted to shrink,which implies that the existing M.pyrifera would be adversely impacted.These results serve as references for the conservation and utilization of M.pyrifera resource.
文摘The most important marine coastal ecosystem in the Chilean coast are kelp forests.This review is based on ecological studies regarding different aspects of subtidal kelp ecosystems along the Chilean coast.It highlights the most interesting findings in(1)biology of subtidal kelp in Chile,with particular reference to(2)habitats formed by kelp,and considered the successful examples and promising results in the(3)kelp as an industrial resource(Biotechnological approach of kelps and aquaculture).The impact caused by(4)El Niño-Southern Oscillation is discussed as an important climatic event that could help to forecast the future of the kelp ecosystem.In addition,this literature review outlines the knowledge gaps on subtidal kelp along the Southeast Pacific Coast of Chile,so that research can be strengthened in the future.
文摘Stable isotope analysis was used to determine the relative dietary importance of kelp-derived detritus to plankton and benthic organisms along a gradient of kelp abundance driven by recovering sea otter populations along the west coast of Vancouver Island (WCVI), Canada. The study used region-specific kelp isotope values (δ<sup>13</sup>C and δ<sup>15</sup>N) and season-specific phytoplankton isotope values to model dietary contributions of kelp-derived detritus (KDD). In general, KDD contributions were moderate to high in most plankton size fractions during the summer and decreased during the winter, particularly in the kelp sparse region. Hypothesized regional and spatial (distance from the coast) differences in kelp detritus contributions to zooplankton w<span style="white-space:normal;"><span style="font-family:;" "="">ere<span style="white-space:normal;"><span style="font-family:;" "=""> not evident. Modeled estimates of the KDD contribution to benthic invertebrates w<span style="white-space:normal;"><span style="font-family:;" "="">ere<span style="white-space:normal;"><span style="font-family:;" "=""> high (>40%) and independent of the organism size, among regions and between seasons, with the exception of Astraea gibberosa in the kelp abundant region. Local oceanography, natural kelp isotope signature variation, and significant overlap between kelps’ and blooming phytoplankton isotope values led to a large uncertainty in the assessed KDD contributions in benthic organisms. These results highlighted the importance of the KDD as a widespread and stable year-round food source in coastal kelp populated regions.
文摘Several polyculture models of scallop Chlamys farreri and kelp Laminaria japonica currently employedin Sungo Bay and other parts of northern China are described in this paper. Economic benefits of differ-ent polyculture models are analysed based on the growth rate, culture density and market price. In addi-tion, site selection, critical environmental conditions and polyculture problems are discussed in detail.
基金Supported by the National Key Technologies R&D Program of China(No.2011BAD13B02)the NSFC-Shandong Joint Fund for Marine Science Research Centers(No.U1406403)the Marine Strategic Priority Research Program of Chinese Academy of Sciences(No.XDA11020704)
文摘Bivalves and seaweeds are important cleaners that are widely used in integrated multi-trophic aquaculture （IMTA） systems. A beneficial relationship between seaweed and bivalve in the seaweed- based IMTA system has been confirmed, but the trophic importance of seaweed-derived particulate organic materials to the co-cultured bivalve is still unclear. We evaluated the trophic importance of the kelp Saccharinajaponica to the co-cultured scallop Chlamysfarreri in a typical IMTA farm in Sungo Bay （Weihai, North China）. The dynamics of detritus carbon in the water were monitored during the culturing period. The proportion of kelp-derived organic matter in the diet of the co-cultured scallop was assessed via the stable carbon isotope method. Results showed that the detritus carbon in the water ranged from 75.52 to 265.19 ~tg/L, which was 25.6% to 73.8% of total particulate organic carbon （TPOC） during the study period. The amount of detritus carbon and its proportion in the TPOC changed throughout the culture cycle of the kelp. Stable carbon isotope analysis showed that the cultured scallop obtained 14.1% to 42.8% of its tissue carbon from the kelp, and that the percentages were closely correlated with the proportion of detritus carbon in the water （F=0.993, P=0.003）. Evaluation showed that for 17 000 tons （wet weight） of annual scallop production, the kelp contributed about 139.3 tons of carbon （535.8 tons of dry mass）. This confirms that cultured kelp plays a similar trophic role in IMTA systems as it does in a natural kelp bed. It is a major contributor to the detritus pool and supplies a vital food source to filter-feeding scallops in the IMTA system, especially during winter and early spring when phytoplankton are scarce.
基金Supported by the National Key R&D Program of China(No.2019YFD0900803)the National Natural Science Foundation of China(No.41876185)+2 种基金the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)(No.2018SDKJ0502)the Youth Talent Program Supported by Laboratory for Marine Fisheries Science and Food Production Processes of Pilot National Laboratory for Marine Science and Technology(Qingdao)(No.2018-MFS-T13)the Modern Agro-industry Technology Research System(No.CARS-49)。
文摘Integrated Multi-Trophic Aquaculture(IMTA)is an effective method for sustainable aquaculture as species from different trophic levels could reduce negative effects from fed species in the environment.A proper proportion of different trophic species in an IMTA system could improve the aquaculture production and environmental sustainability.At present,research on the proper proportions for farming species is scarce.We investigated the effects of IMTA modes of oyster(Crassostrea gigas)and kelp(Saccharina japonica)in different weight ratios on water quality and carbonate system in a closed enclosure experiment for three days in the Sanggou Bay in Shandong Province,China,in December 2017.Nine collocation modes in oyster:kelp weight ratio were tested showing as 24:3,24:2,24:1,16:3,16:2,16:1,8:3,8:2,and 8:1.The water parameters were determined at 17:00 on Day 1(D1),and 6:00 and 17:00 on Days 2(D2)and 3(D3).As two-way ANOVA showed,all increased parameters(dissolved oxygen(DO),pH,chl a,the carbonate system and pCO2)were significantly related to oyster-kelp modes,and interaction between modes and time were also significant(P<0.05).On the 3th day,the 8:3 mode was the highest in DO,pH,chl a,CO32-(P<0.05),and dissolved inorganic carbon(DIC),HCO3-,CO2,and pCO2 were the lowest(P<0.05).According to previous references and the results of this study,the appropriate oyster:kelp proportion at the beginning of winter is from 8:2 to 8:3.The results of this study may help government to optimize the aquaculture structure of Sanggou Bay.
基金supported by the Natural Science Foundation of Fujian Province (2020J01558)the Expert Workstation of Fuzhou Hailin Food Co., Ltd
文摘Laminaria japonica Aresch as an edible and medicine dual-purpose marine algae,has been gradually accepted by people.Tofu was Chinese traditional food,combining kelp function ingredients with tofu has practical value.The extraction-optimization methodology and the sequences of kelp antihypertensive peptide(KAP)were investigated.The sensory,whiteness,water retention ability(WRA)and microstructure of KAP tofu were evaluated.The scanning electron microscope(SEM)results showed KAP addition(20%)significantly improved the tofu texture,with a denser network composed of relatively even pores.Compared with gypsum tofu and glucose-δ-interior fat(GDL)tofu,KAP tofu had significantly higher WRA(94.49±0.49)%and sensory evaluation score value(92.54±0.52),and a significantly lower IC_(50) value at(2.06±0.04)mg/mL(P<0.05).The maximum IC_(50) value(4.14 mg/mL)of kelp enzymatic hydrolysate was observed at enzyme(at an alkaline protease:trypsin ratio of 2:1)concentration 1.5%,temperature 55℃ and time 2 h.The sequences of 8 identified peptides were Lys-Tyr,Phe-Tyr,Gly-Lys-Tyr,Ala-Lys-Tyr,Ser-Lys-Thr-Tyr,Lys-Lys-Phe-Tyr,Lys-Phe-Lys-Tyr and Ala-Lys-Tyr-Ser-Tyr with IC_(50) values of 5.24,4.83,7.94,7.52,20.63,15.33,10.73 and 2.42μmol/L,respectively.These results indicated the potential use of KAP tofu as a valuable resource for development of functional foods.
文摘An artificial way to prevent and cure the iodine deficiency disorder(IDD) through ameliorating the biogeochemical environment of the regions where iodine is deficient was put forward. In this paper, the concrete method to achieve this way was given and its feasibility is confirmed by test.
文摘The kelp grouper (Epinephelus bruneus), belonging to one of the largest genera among the subfamily Epinephelinae, is a commercially important fish in Japan. There are limited data about the genomics of this species. To provide tools for addressing both population genetics studies and gene mapping, dito pentanucleotide simple sequence repeat (SSR) markers were developed using 454 pyrosequencing. Among the 1466 SSR markers developed, 1244 primer sets produced strong PCR products, of which 905 (72.7%) were polymorphic in kelp grouper. Cross-species utility of the 905 polymorphic SSR markers was tested in four additional Epinephelinae species of Hyporthodus septemfasciatus, Plectropomus leopardus, Epinephelus lanceolatus and Epinephelus coioides. Results revealed that, respectively, 401 (44.3%), 136 (15.0%), 434 (49.0%) and 538 (59.4%) SSRs showed specific polymorphic products. Of these, 40 SSR markers (33 di-, 1 tri- and 6 tetra-nucleotides) showed polymorphism in all species tested. Additionally, three AGAT SSR motifs which accounted for 42.9% of the nondi-nucleotide markers were found in the 40 SSR markers. This indicates that the AGAT SSR motif has a high potential as a highly versatile SSR marker in grouper Epinephelinae. The SSR markers developed in this study can be employed to obtain reliable genetic variability estimates for groupers (Epinephelinae).
文摘Flexible energy-storage devices play a critical role in the development of portable, flexible and wearable electronics. In addition, biological materials including plants or plant-based materials are known for their safety, biodegradability, biocompatibility, environmental benignancy, and low cost. With respect to these advances, a flexible alkaline zinc-manganese dioxide (Zn-MnO2) battery is fabricated with a kelp-based electrolyte in this study. To the best of our knowledge, pure kelp is utilized as a semi-solid electrolyte for flexible Zn-MnO2 alkaline batteries for the first time, with which the as-assembled battery exhibited a specific capacity of 60 mA·h and could discharge for 120 h. Furthermore, the as-assembled Zn-MnO2 battery can be bent into a ring-shape and power a light-emitting diode screen, showing promising potential for the practical application in the future flexible, portable and biodegradable electronic devices.
文摘The release process and influencing factors of bioactive iodine of kelp are systemically studied by leaching experiment. The results showed that the bioactive iodine of kelp can be released rapidly and the principal form of iodine in lixivium is I -. There is a dynamic process between the release and absorption of iodine. With the increase of leaching water, the gross amounts of released iodine rise. There also exists a transforming process among I -, IO - 3 and organic iodine in lixivium.
基金Suported by the Spanish Ministry of Science and Technology Project (No. AGL2002-01285)a PhD Scholarship of the University of A Corua
文摘The aim of the study is to examine the influence of the outplanting time on the cultivation of the kelp Laminaria saccharina in the northwestern Spain, close to its southern limit of distribution in the North Atlantic coast. We compared two L. saccharina cultures outplanted in February and December. We found that the starting time of outplanting affects to the yield and the substantiality value, as well as the growth rate. The yield, the substantiality value and the growth rates of L. saccharina sporophytes obtained for the trial outplanted in December were significantly higher than those in February. These results can be mainly explained by the effect of temperature on the growth of L. saccharina sporophytes during cultivation. On the other hand, our results show that even in the southern limit of distribution of L. saccharina, the cultivation of this species was very profitable in high yield and quality.
基金Sponsored by the National Natural Science Foundation of China(Grant Nos.30571244 and 30870003)China National Key Technology R&D Program(Grant No.2009BADA0B05-4)
文摘Ecological prevention and control of plant disease is very important in sustainable agriculture.Adjusting soil p H value and fertilizing organic microbial fertilizer are two effective measures in this process.Kelp residue contains a large amount of organic compounds and alkaline metal ions.The bio-control Bacillus amyloliquefaciens strain Hitwh-BA2 was inoculated into kelp residue medium to produce kelp residue microbial fertilizer.Acidic soil and alkaline soil were used to study the effect of kelp residue microbial fertilizer on soil p H and soil suppressive activity.Tip-culture method was used to determine soil leachate suppressive activity,which characterized the soil suppressive activity.Results showed that fertilizing kelp residue microbial fertilizer had increased the soil p H and soil suppressive ability significantly,which was verified by peanut validation experiments as well.Peanut potting experiments proved that fertilizing kelp residue microbial fertilizer not only improved the yield of peanuts obviously,but also reduced the amount of Aspergillus parasiticus 95 in peanut geocarposphere soil significantly.Results also showed that fertilizing kelp residue microbial fertilizer was effective in reducing A.parasiticus 95 infection rate.So the kelp residue microbial fertilizer has good potential application prospect on ecological prevention and control of plant disease.
基金supported by the Special Fund for Clinical Scientific Research of Shandong Medical Association(No.YXH2020ZX058).
文摘This study was carried out explore the mechanism underlying the inhibition of platelet activation by kelp fucoidans in deep venous thrombosis(DVT)mouse.In the control and sham mice,the walls of deep vein were regular and smooth with intact intima,myometrium and adventitia.The blood vessel was wrapped with the tissue and there was no thrombosis in the lumen.In the DVT model,the wall was uneven with thicken intima,myometrium and adventitia.After treated with fucoidans LF1 and LF2,the thrombus was dissolved and the blood vessel was recanalized.Compared with the control group,the ROS content,ET-1 and VWF content and the expression of PKC-βand NF-κB in the model were significantly higher(P<0.05);these levels were significantly reduced following treatments with LF2 and LF1.Compared with H_(2)O_(2)treated-HUVECs,combined LF1 and LF2 treatment resulted in significant decrease in the expression of PKC-β,NF-κB,VWF and TM protein(P<0.05).It is clear that LF1 and LF2 reduces DVT-induced ET-1,VWF and TM expressions and production of ROS,thus inhibiting the activation of PKC-β/NF-κB signal pathway and the activation of coagulation system and ultimately reducing the formation of venous thrombus.
文摘In order to study the development status of kelp industry in Rongcheng in recent years, the centralization degree of kelp industry clusters and the comparative advantage of kelp industry in Rongcheng, Xiapu and Jinpu from 2010 to 2020 were compared and analyzed by indicators such as location quotient index, industrial agglomeration index, coefficient of concentration, scale advantage index, efficiency advantage index, and comprehensive advantage index, and the deep-seated factors affecting the competitiveness of kelp industry in Rongcheng were analyzed. Meanwhile, measures of improving the competitiveness of the kelp industry cluster in Rongcheng were put forward.
文摘Dried kelp is one of the most important Japanese foods mainly used as base ingredient. Holdfasts, or rhizoids, of kelp are usually discarded, as rocks, shells, ropes and other contaminants are entangled among them. The purpose of the present study was to examine whether the preparation of activated carbon from holdfasts of <em>Saccharina japonica</em>, a popular kelp harvested in Japan, is possible by a chemical activation technique utilizing zinc chloride, and to study the influence of the process parameters, such as carbonization temperature and the composition of the activator. Under the examined experimental conditions, maximum specific surface area of 1.7 × 10<sup>3</sup> m<sup>2</sup>/g, larger than that of a commercial activated carbon, was observed at 600℃ and the zinc chloride composition of 75 mass%.
文摘At present, the marine environment is faced with extremely serious eutrophication. The kelp＇s ability of taking in nitrogen and phosphorus can be fully performed so that the eutrophication problem can be effectively treated and solved. Based on the analysis of kelp＇s absorption of nutritive salt from aquaculture sewage, this paper further explores the bioremediation of kelp in aquaculture sea area with eutrophication in the hope of providing valuable reference for the aquaculture industry of BohaiSea.
