目的:探究白内障患者人工晶状体(IOL)植入后Kappa角变化趋势及与IOL稳定性的关系。方法:选择2023-02/2024-01于我院接受IOL植入的白内障患者120例120眼为研究对象,其中左眼56眼,右眼64眼。比较手术前后眼科检查结果、Kappa角的分布,以...目的:探究白内障患者人工晶状体(IOL)植入后Kappa角变化趋势及与IOL稳定性的关系。方法:选择2023-02/2024-01于我院接受IOL植入的白内障患者120例120眼为研究对象,其中左眼56眼,右眼64眼。比较手术前后眼科检查结果、Kappa角的分布,以及术后不同时点Kappa角变化和IOL稳定性。Pearson相关及多重线性回归分析IOL旋转稳定性与各眼科检查指标的相关性。多元线性回归分析Kappa角与IOL稳定性的相关性并绘制变化趋势图。结果:术后Kappa角逐渐减小且减小程度逐渐降低(均P<0.05),IOL旋转度数也逐渐减小(P<0.05)。术后2 mo IOL旋转度数与术后1 d AL、LT、K1、K2、PD、Kappa角呈正相关(均P<0.05),与术后1 d ACD呈负相关(均P<0.05)。术后1 d AL、ACD、PD、Kappa角对IOL旋转度数产生显著影响(均P<0.05)。随着Kappa角增大,IOL旋转度数逐渐增大,即IOL稳定性逐渐降低(P<0.05)。结论:白内障患者IOL植入后Kappa角显著减小,前期减小幅度较大,而后趋于稳定,IOL稳定性随Kappa角的增大而降低。展开更多
AIM:To investigate how angles kappa and alpha affect postoperative visual quality in patients with multifocal intraocular lens(mIOLs)implantation.METHODS:Retrospective cases series.A total of 46 patients(46 eyes)who u...AIM:To investigate how angles kappa and alpha affect postoperative visual quality in patients with multifocal intraocular lens(mIOLs)implantation.METHODS:Retrospective cases series.A total of 46 patients(46 eyes)who underwent phacoemulsification were subsumed.The correlation between Preoperative angles kappa and alpha,wave-front aberrations and objective visual quality of cornea,internal,and total eye after surgery were analyzed using iTrace.RESULTS:The magnitude of angle kappa was negatively correlated with internal and total modulation transfer function(MTF)at 3 mm;the magnitude of angle kappa was positively correlated with astigmatism,trefoil,higher-order aberrations(HOAs)of both internal and total eye at 3 mm.The magnitude of angle alpha was negatively correlated with total MTF and total Strehl ratio at 3 mm.The magnitude of angle alpha was positively correlated with corneal coma at 5 mm,internal astigmatism at both 3 mm and 5 mm,and total spherical aberration(SA)at 3 mm.Multivariate linear regression analysis showed that,among candidate independent variables(kappa,alpha,astigmatism,SA,coma,trefoil,and HOAs),astigmatism is the only independent factor for altering corneal MTF at 3 mm and 5 mm;astigmatism and HOAs emerged as independent factors for altering internal MTF at 3 mm and 5 mm,and total MTF at 3 mm;astigmatism,SA and HOAs emerged as independent factors for altering total MTF at 5 mm.CONCLUSION:With greater preoperative angle kappa or angle alpha,patients who accept mIOL implantation tend to have larger internal astigmatism and HOAs,which resulting in poor visual quality,especially those with small pupil size.展开更多
AIM:To explore the effect of Alpha angle and Kappa angle before multifocal intraocular lenses(MIOLs)implantation on postoperative visual quality of patients.METHODS:Before and 3mo after cataract surgery,Alpha angle an...AIM:To explore the effect of Alpha angle and Kappa angle before multifocal intraocular lenses(MIOLs)implantation on postoperative visual quality of patients.METHODS:Before and 3mo after cataract surgery,Alpha angle and Kappa angle were collected using IOL Master 700,iTrace,and Pentacam for clinical observation.Postoperative visual quality indicators,including high-order aberrations(HOA),modulation transfer function(MTF)and point spread function(PSF),were collected using iTrace.multiple linear regression analysis was used to analyze the correlation of the Kappa angle and the Alpha angle with age,axial length(AL),anterior chamber depth(ACD),keratometry(K),lens thickness(LT)and corneal white to white distance(WTW).Pearson correlation coefficient was used to analyze the correlation between Alpha angle and Kappa angle;Bland Altman analysis was used to evaluate the consistency of pairwise detection results of three instruments.RESULTS:The Alpha angle was modeled as Alpha=2.230+0.003×age-0.036×AL-0.025×K-0.058×WTW and the Kappa angle was modeled as Kappa=0.685+0.003×age-0.013×K-0.061×WTW.The correlation between the total Alpha angle and Kappa angle of the three instruments was weakly positive(r=0.291,P=0.000).Comparing the measurement of Alpha angle and Kappa angle using three instruments,only IOL Master 700 and iTrace showed good consistency in measuring Kappa angle(P=0.4254).After 3mo of surgery,the Alpha angle and Kappa angle significantly decreased(P=0.011,0.018;P=0.008,0.036).△Kappa=1.136-0.021×AL-0.013×K.Kappa angle could positively predict HOA(β=0.18,P=0.000),MTF(β=0.171,P=0.000),PSF(β=0.088,P=0.000),Alpha angle cannot(P>0.05).CONCLUSION:The patients with older age,flatter K and shorter WTW should be alert to the possibility of larger Alpha angle and Kappa angle.Alpha angle should also consider the factor of AL.When selecting patients with MIOLs implantation,there is no need to consider the Alpha angle.Careful consideration should be given to the Kappa angle,and the preoperative standard of<0.5 mm can refer to△Kappa=1.136-0.021×AL-0.013×K and be appropriately relaxed.展开更多
BACKGROUND Radiation induced pulmonary fibrosis(RIPF)is a long-term lung condition with a bleak outlook and few treatment possibilities.Mesenchymal stem cells(MSCs)-derived exosomes(MSCs-exosomes)possess tissue repair...BACKGROUND Radiation induced pulmonary fibrosis(RIPF)is a long-term lung condition with a bleak outlook and few treatment possibilities.Mesenchymal stem cells(MSCs)-derived exosomes(MSCs-exosomes)possess tissue repair and regenerative pro-perties,but their exact mechanisms in RIPF remain unclear.This study explores whether MSCs-exosomes can alleviate RIPF by modulating inflammation,ex-tracellular matrix(ECM)accumulation,and epithelial-mesenchymal transition(EMT)via the protein kinase B(Akt)/nuclear factor kappa B(NF-κB)pathway.Sprague-Dawley rats were received 30 Gy X-ray radiation on the right chest to induce RIPF,while RLE-6TN and BEAS-2B cell lines were exposed to 10 Gy X-rays.Using differential centrifugation,MSCs-exosomes were isolated,and their protective effects were examined both in vivo and in vitro.Inflammatory cytokine concentrations were measured using Luminex liquid chip detection and enzyme linked immunosorbent assay.ECM and EMT-related proteins were analyzed using immunohistochemistry,western blotting,and real-time quantitative polymerase chain reaction.Western blotting and immunohistochemistry were also used to investigate the mechanisms underlying MSCs-exosomes’effects in RIPF.RESULTS Administration of MSCs-exosomes significantly mitigated RIPF,reduced collagen deposition,and decreased levels of various inflammatory cytokines.Additionally,MSCs-exosomes prevented radiation-induced ECM accumulation and EMT.Treatment with MSCs-exosomes notably promoted cell proliferation,suppressed inflammation,and reversed ECM deposition and EMT in radiation-exposed alveolar epithelial cells.Mechanistic analysis further revealed that MSCs-exosomes exerted their anti-RIPF effects by inhibiting the Akt/NF-κB pathway,as shown in both in vivo and in vitro models.CONCLUSION MSCs-exosomes mitigate RIPF by suppressing inflammation,ECM deposition,and EMT through Akt/NF-κB inhibition,highlighting their potential as a therapeutic strategy.展开更多
BACKGROUND Mesenchymal stromal cells(MSCs)are renowned for their immunosuppressive properties,which make them widely used in managing excessive inflammation.Although CD146+and CD146-MSCs exhibit similar morphological ...BACKGROUND Mesenchymal stromal cells(MSCs)are renowned for their immunosuppressive properties,which make them widely used in managing excessive inflammation.Although CD146+and CD146-MSCs exhibit similar morphological traits and surface marker expression levels,the specific characteristics and differential regulatory mechanisms of these two subtypes remain poorly understood.This knowledge gap has limited the precise application of MSCs in targeted thera-peutic strategies.AIM To compare the functional differences between CD146+and CD146-MSCs and investigate the underlying mechanisms.METHODS In this study,magnetic beads were used to sort umbilical cord-derived MSCs into CD146+and CD146-subsets.The pro-angiogenic factors(hepatocyte growth factor,prostaglandin E2,vascular endothelial growth factor,angiopoietin-1)production and immunomodulatory effects on T lymphocyte subsets were evaluated in vitro.The therapeutic efficacy was assessed in an acute respiratory distress syndrome(ARDS)mouse model via tail vein injection.RESULTS Cytokine secretion and angiogenesis:CD146+MSCs significantly increased the production of hepatocyte growth factor,prostaglandin E2,vascular endothelial growth factor,and angiopoietin-1 and exhibited increased pro-angiogenic activity in vitro.Immunomodulatory effects:CD146+MSCs potently inhibited the differentiation and proliferation of pro-inflammatory T helper type 1/T helper type 17 cells while promoting the expansion of regulatory T cells during T lymphocyte activation.ARDS therapy:In a mouse ARDS model,compared with CD146-MSCs,CD146+MSCs demonstrated superior therapeutic efficacy,as evidenced by improved clinical scores.Mechanistically,CD146+MSCs activated the nuclear factor kappa B pathway,upregulated cyclooxygenase 2 expression,and facilitated damaged epithelial cell repair.CONCLUSION CD146+MSCs show stronger ARDS therapeutic potential than CD146-MSCs via pro-angiogenic/immunomodulatory traits.Nuclear factor kappa B/cyclooxygenase 2 activation aids epithelial repair,highlighting CD146+MSCs as promising targets.展开更多
文摘目的:探究白内障患者人工晶状体(IOL)植入后Kappa角变化趋势及与IOL稳定性的关系。方法:选择2023-02/2024-01于我院接受IOL植入的白内障患者120例120眼为研究对象,其中左眼56眼,右眼64眼。比较手术前后眼科检查结果、Kappa角的分布,以及术后不同时点Kappa角变化和IOL稳定性。Pearson相关及多重线性回归分析IOL旋转稳定性与各眼科检查指标的相关性。多元线性回归分析Kappa角与IOL稳定性的相关性并绘制变化趋势图。结果:术后Kappa角逐渐减小且减小程度逐渐降低(均P<0.05),IOL旋转度数也逐渐减小(P<0.05)。术后2 mo IOL旋转度数与术后1 d AL、LT、K1、K2、PD、Kappa角呈正相关(均P<0.05),与术后1 d ACD呈负相关(均P<0.05)。术后1 d AL、ACD、PD、Kappa角对IOL旋转度数产生显著影响(均P<0.05)。随着Kappa角增大,IOL旋转度数逐渐增大,即IOL稳定性逐渐降低(P<0.05)。结论:白内障患者IOL植入后Kappa角显著减小,前期减小幅度较大,而后趋于稳定,IOL稳定性随Kappa角的增大而降低。
文摘AIM:To investigate how angles kappa and alpha affect postoperative visual quality in patients with multifocal intraocular lens(mIOLs)implantation.METHODS:Retrospective cases series.A total of 46 patients(46 eyes)who underwent phacoemulsification were subsumed.The correlation between Preoperative angles kappa and alpha,wave-front aberrations and objective visual quality of cornea,internal,and total eye after surgery were analyzed using iTrace.RESULTS:The magnitude of angle kappa was negatively correlated with internal and total modulation transfer function(MTF)at 3 mm;the magnitude of angle kappa was positively correlated with astigmatism,trefoil,higher-order aberrations(HOAs)of both internal and total eye at 3 mm.The magnitude of angle alpha was negatively correlated with total MTF and total Strehl ratio at 3 mm.The magnitude of angle alpha was positively correlated with corneal coma at 5 mm,internal astigmatism at both 3 mm and 5 mm,and total spherical aberration(SA)at 3 mm.Multivariate linear regression analysis showed that,among candidate independent variables(kappa,alpha,astigmatism,SA,coma,trefoil,and HOAs),astigmatism is the only independent factor for altering corneal MTF at 3 mm and 5 mm;astigmatism and HOAs emerged as independent factors for altering internal MTF at 3 mm and 5 mm,and total MTF at 3 mm;astigmatism,SA and HOAs emerged as independent factors for altering total MTF at 5 mm.CONCLUSION:With greater preoperative angle kappa or angle alpha,patients who accept mIOL implantation tend to have larger internal astigmatism and HOAs,which resulting in poor visual quality,especially those with small pupil size.
基金Supported by National Natural Science Foundation of China(No.81902751).
文摘AIM:To explore the effect of Alpha angle and Kappa angle before multifocal intraocular lenses(MIOLs)implantation on postoperative visual quality of patients.METHODS:Before and 3mo after cataract surgery,Alpha angle and Kappa angle were collected using IOL Master 700,iTrace,and Pentacam for clinical observation.Postoperative visual quality indicators,including high-order aberrations(HOA),modulation transfer function(MTF)and point spread function(PSF),were collected using iTrace.multiple linear regression analysis was used to analyze the correlation of the Kappa angle and the Alpha angle with age,axial length(AL),anterior chamber depth(ACD),keratometry(K),lens thickness(LT)and corneal white to white distance(WTW).Pearson correlation coefficient was used to analyze the correlation between Alpha angle and Kappa angle;Bland Altman analysis was used to evaluate the consistency of pairwise detection results of three instruments.RESULTS:The Alpha angle was modeled as Alpha=2.230+0.003×age-0.036×AL-0.025×K-0.058×WTW and the Kappa angle was modeled as Kappa=0.685+0.003×age-0.013×K-0.061×WTW.The correlation between the total Alpha angle and Kappa angle of the three instruments was weakly positive(r=0.291,P=0.000).Comparing the measurement of Alpha angle and Kappa angle using three instruments,only IOL Master 700 and iTrace showed good consistency in measuring Kappa angle(P=0.4254).After 3mo of surgery,the Alpha angle and Kappa angle significantly decreased(P=0.011,0.018;P=0.008,0.036).△Kappa=1.136-0.021×AL-0.013×K.Kappa angle could positively predict HOA(β=0.18,P=0.000),MTF(β=0.171,P=0.000),PSF(β=0.088,P=0.000),Alpha angle cannot(P>0.05).CONCLUSION:The patients with older age,flatter K and shorter WTW should be alert to the possibility of larger Alpha angle and Kappa angle.Alpha angle should also consider the factor of AL.When selecting patients with MIOLs implantation,there is no need to consider the Alpha angle.Careful consideration should be given to the Kappa angle,and the preoperative standard of<0.5 mm can refer to△Kappa=1.136-0.021×AL-0.013×K and be appropriately relaxed.
基金Supported by Natural Science Foundation of Liaoning Province,No.2024-MS-250.
文摘BACKGROUND Radiation induced pulmonary fibrosis(RIPF)is a long-term lung condition with a bleak outlook and few treatment possibilities.Mesenchymal stem cells(MSCs)-derived exosomes(MSCs-exosomes)possess tissue repair and regenerative pro-perties,but their exact mechanisms in RIPF remain unclear.This study explores whether MSCs-exosomes can alleviate RIPF by modulating inflammation,ex-tracellular matrix(ECM)accumulation,and epithelial-mesenchymal transition(EMT)via the protein kinase B(Akt)/nuclear factor kappa B(NF-κB)pathway.Sprague-Dawley rats were received 30 Gy X-ray radiation on the right chest to induce RIPF,while RLE-6TN and BEAS-2B cell lines were exposed to 10 Gy X-rays.Using differential centrifugation,MSCs-exosomes were isolated,and their protective effects were examined both in vivo and in vitro.Inflammatory cytokine concentrations were measured using Luminex liquid chip detection and enzyme linked immunosorbent assay.ECM and EMT-related proteins were analyzed using immunohistochemistry,western blotting,and real-time quantitative polymerase chain reaction.Western blotting and immunohistochemistry were also used to investigate the mechanisms underlying MSCs-exosomes’effects in RIPF.RESULTS Administration of MSCs-exosomes significantly mitigated RIPF,reduced collagen deposition,and decreased levels of various inflammatory cytokines.Additionally,MSCs-exosomes prevented radiation-induced ECM accumulation and EMT.Treatment with MSCs-exosomes notably promoted cell proliferation,suppressed inflammation,and reversed ECM deposition and EMT in radiation-exposed alveolar epithelial cells.Mechanistic analysis further revealed that MSCs-exosomes exerted their anti-RIPF effects by inhibiting the Akt/NF-κB pathway,as shown in both in vivo and in vitro models.CONCLUSION MSCs-exosomes mitigate RIPF by suppressing inflammation,ECM deposition,and EMT through Akt/NF-κB inhibition,highlighting their potential as a therapeutic strategy.
基金Supported by the Science and Technology SMEs Innovation Capacity Improvement Project of Shandong Province,No.2022TSGC1004National Key R&D Program of China,No.2021YFA1101502。
文摘BACKGROUND Mesenchymal stromal cells(MSCs)are renowned for their immunosuppressive properties,which make them widely used in managing excessive inflammation.Although CD146+and CD146-MSCs exhibit similar morphological traits and surface marker expression levels,the specific characteristics and differential regulatory mechanisms of these two subtypes remain poorly understood.This knowledge gap has limited the precise application of MSCs in targeted thera-peutic strategies.AIM To compare the functional differences between CD146+and CD146-MSCs and investigate the underlying mechanisms.METHODS In this study,magnetic beads were used to sort umbilical cord-derived MSCs into CD146+and CD146-subsets.The pro-angiogenic factors(hepatocyte growth factor,prostaglandin E2,vascular endothelial growth factor,angiopoietin-1)production and immunomodulatory effects on T lymphocyte subsets were evaluated in vitro.The therapeutic efficacy was assessed in an acute respiratory distress syndrome(ARDS)mouse model via tail vein injection.RESULTS Cytokine secretion and angiogenesis:CD146+MSCs significantly increased the production of hepatocyte growth factor,prostaglandin E2,vascular endothelial growth factor,and angiopoietin-1 and exhibited increased pro-angiogenic activity in vitro.Immunomodulatory effects:CD146+MSCs potently inhibited the differentiation and proliferation of pro-inflammatory T helper type 1/T helper type 17 cells while promoting the expansion of regulatory T cells during T lymphocyte activation.ARDS therapy:In a mouse ARDS model,compared with CD146-MSCs,CD146+MSCs demonstrated superior therapeutic efficacy,as evidenced by improved clinical scores.Mechanistically,CD146+MSCs activated the nuclear factor kappa B pathway,upregulated cyclooxygenase 2 expression,and facilitated damaged epithelial cell repair.CONCLUSION CD146+MSCs show stronger ARDS therapeutic potential than CD146-MSCs via pro-angiogenic/immunomodulatory traits.Nuclear factor kappa B/cyclooxygenase 2 activation aids epithelial repair,highlighting CD146+MSCs as promising targets.
