The conversion of saline-alkali soils into paddy fields for long-term rice cultivation involves multiple disturbances,and as a result,soil microbial communities are altered to adapt to changing environmental condition...The conversion of saline-alkali soils into paddy fields for long-term rice cultivation involves multiple disturbances,and as a result,soil microbial communities are altered to adapt to changing environmental conditions.However,a comprehensive understanding of the succession of soil bacterial communities that occurs during this process is still lacking.In the present study,we utilized data obtained from paddy fields of different rice cultivation years(0-23 years)to investigate the compositional and functional succession of soil bacterial communities.We focused on core bacterial taxa that were specifically enriched at different successional stages.Generalized joint attribute modeling(GJAM)was used to identify core bacterial taxa.Results indicated that the bare saline-alkali soil(0 year,prior to any rice cultivation)shared few core amplicon sequence variants(ASVs)with paddy fields.In the bare saline-alkali soil,Longimicrobiaceae from the phylum Gemmatimonadetes was dominant,while the dominance was subsequently replaced by Burkholderiaceae and Pedosphaeraceae--phyla affiliated with Proteobacteria and Verrucomicrobia--after 5 and 23 years of rice cultivation,respectively.The relative abundances of nitrogen metabolism functions in the core bacterial communities of the bare saline-alkali soil were higher than those at other successional stages,while sulfur metabolism functions exhibited the opposite trend.These indicated that the role of the core bacterial taxa in mediating nutrient cycling also evolved and adapted to changing soil conditions as rice cultivation was established.Redundancy analysis(RDA)indicated that the composition of the core bacterial community in paddy fields with rice cultivation for 0,2 and 4,6,8,10,and 12,and 20 and 23 years were driven by soil nitrate nitrogen content,pH,available phosphorus content,and the ratio of total carbon to total nitrogen,respectively.In summary,the present study provides insights into the succession of soil bacterial communities and core bacterial taxa that occurs during long-term rice cultivation.展开更多
基金supported by the National Natural Science Foundation of China(Nos.32371734,42007034,41920104008,and U22A20593)the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDA28020400)+2 种基金the Youth Innovation Promotion Association of Chinese Academy of Sciences(No.2023205)the Young Scientist Group Project of Northeast Institute of Geography and Agroecology of China(No.2022QNXZ04)the Science and Technology Development Project of Jilin Province of China(No.YDZJ202101ZYTS006).
文摘The conversion of saline-alkali soils into paddy fields for long-term rice cultivation involves multiple disturbances,and as a result,soil microbial communities are altered to adapt to changing environmental conditions.However,a comprehensive understanding of the succession of soil bacterial communities that occurs during this process is still lacking.In the present study,we utilized data obtained from paddy fields of different rice cultivation years(0-23 years)to investigate the compositional and functional succession of soil bacterial communities.We focused on core bacterial taxa that were specifically enriched at different successional stages.Generalized joint attribute modeling(GJAM)was used to identify core bacterial taxa.Results indicated that the bare saline-alkali soil(0 year,prior to any rice cultivation)shared few core amplicon sequence variants(ASVs)with paddy fields.In the bare saline-alkali soil,Longimicrobiaceae from the phylum Gemmatimonadetes was dominant,while the dominance was subsequently replaced by Burkholderiaceae and Pedosphaeraceae--phyla affiliated with Proteobacteria and Verrucomicrobia--after 5 and 23 years of rice cultivation,respectively.The relative abundances of nitrogen metabolism functions in the core bacterial communities of the bare saline-alkali soil were higher than those at other successional stages,while sulfur metabolism functions exhibited the opposite trend.These indicated that the role of the core bacterial taxa in mediating nutrient cycling also evolved and adapted to changing soil conditions as rice cultivation was established.Redundancy analysis(RDA)indicated that the composition of the core bacterial community in paddy fields with rice cultivation for 0,2 and 4,6,8,10,and 12,and 20 and 23 years were driven by soil nitrate nitrogen content,pH,available phosphorus content,and the ratio of total carbon to total nitrogen,respectively.In summary,the present study provides insights into the succession of soil bacterial communities and core bacterial taxa that occurs during long-term rice cultivation.
