Objective： To prospectively investigate the value of bone scintigraphy on determining the full extent of tumor involvement in jaw bones and to assess the presence of metastases. Methods： This study had local ethical...Objective： To prospectively investigate the value of bone scintigraphy on determining the full extent of tumor involvement in jaw bones and to assess the presence of metastases. Methods： This study had local ethical committee approval, and all patients gave written informed consent. Thirty seven consecutive patients with primary malignant tumor in jaw bones were recruited for the study. Bone scintigraphy was performed in all patients before surgery to measure the full extent of bony involvement, which was compared with histologic findings. Results： Whole body scan revealed one case with multiple bony metastases. Resection specimens of 36 bone neoplasms were pathologically analyzed to identify type and size of each tumor. The lengths of the tumor involvement in jaw bones defined by bone scintigraphy and pathology were 5.62 ± 1.58 cm, 4.48 ± 1.57 cm, respectively （P 〈 0.05）. The tumor negative margins from removed specimens according to bone scintigraphy were pathologically confirmed. With histologic findings as the standard of reference, the accuracy of bone scintigraphy was 100% （36 of 36 patients） in determining the full extent of tumor involvement in jaw bones. Conclusion： Bone scintigraphy tends to offer specific guidelines in determining the appropriate extent of bone resection while entirely clearing the tumor cells and preserving functions whenever possible and in establishing the bony metastases.展开更多
The growth and enlargement of jaw cysts are associated with raised intracystic pressure and bone resorption surrounding the cysts.The major bone-resorbing cells are the osteoclasts.They are acting under the influence ...The growth and enlargement of jaw cysts are associated with raised intracystic pressure and bone resorption surrounding the cysts.The major bone-resorbing cells are the osteoclasts.They are acting under the influence of local bone-resorbing factors: prostaglandins,proteinases and cytokines.It was found that positive pressure enhanced the expression of IL-1αmRNA and protein in epithelial cells of odontogenic keratocyst,and increased the secretion of matrix metalloproteinase and PGE2 in a co-culture of odontogenic keratocyst fibroblasts and epithelial cells.However,the signal intensities for IL-1α mRNA and protein in the epithelium were significantly decreased after marsupialization which relived intracystic pressure.Experimental study indicated that intermittent negative pressure could promote osteogenesis in human bone marrow-derived stroma cells(BMSCs) in vitro.We propose a hypothesis that bone formation around the cyst of the jaws would be stimulated by intracystic negative pressure.展开更多
A new method for filling bone cavity with a graft of frozen-decalcified-defatted-driedallogeneic (FDDDA) cancellous bone was described.This method was used for the treatment of cavi-ties after the enucleation of jaw c...A new method for filling bone cavity with a graft of frozen-decalcified-defatted-driedallogeneic (FDDDA) cancellous bone was described.This method was used for the treatment of cavi-ties after the enucleation of jaw cysts in 10 patients from December 1985 to February 1990.Thewounds of 9 patients healed by first intension,but wound infection occurred in one case postopera-tively.The 9 patients,besides the patient who suffered from wound infection,were followed up for5 to 56 months,with an average of 41.1 months.Evidence of recurrence with jaw cyst.was not ob-served.展开更多
To construct the recombinant adeno-associated virus （rAAV） vector with human bone morphogenetic protein 7 （BMP7） and observe the BMP7 mRNA expression in vitro, BMP7 CDS sequence was cloned into expression plasmid ...To construct the recombinant adeno-associated virus （rAAV） vector with human bone morphogenetic protein 7 （BMP7） and observe the BMP7 mRNA expression in vitro, BMP7 CDS sequence was cloned into expression plasmid pAAV-MCS of AAV Helper Free System. The recombinant plasmid was identified with enzyme digestion and sequencing. The recombinant plasmid, pAAV-RC, pHelper were co-transfected into AAV-293 cells according to the calcium phosphate-based protocol. The viral stock was collected by 4 rounds of freeze/thaw. After purified and concentrated, the recombinant virus titer was determined by dot-blot assay. HEK293 cells were transfected with the recombinant virus at different MOI, and the expression of BMP7 mRNA was detected by RT-PCR. The results showed rAAV-BMP7 was constructed and packaged successfully. The physical particle titer was 2.5×10^11 vector genomes/mL. There was different expression level of BMP7 mRNA after transfecton. These data suggested that recombinant AAV mediated a stable expression of hBMP7 mRNA in 293 cells. The AAV production method may pave the way of an effective strategy for the jaw bone defection around dental implants.展开更多
Background: The diversity of benign jaw tumours may cause difficulty in a correct diagnosis and insti-tution of an appropriate treatment. Data on the prevalence of these tumours is scarce from the Afri-can continent. ...Background: The diversity of benign jaw tumours may cause difficulty in a correct diagnosis and insti-tution of an appropriate treatment. Data on the prevalence of these tumours is scarce from the Afri-can continent. We present a 19-year audit of benign jaw tumours and tumour-like lesions at a University teaching hospital in Nairobi, Kenya. Methods: Histo-pathological records were retrieved and re-examined from the Department of Oral and Maxillofacial pathology, University of Nairobi from 1992 to 2011. The jaw tumours were classified according to the latest WHO classification. Results: During the 19-year audit, 4257 biopsies were processed of which 597 (14.02%) were jaw tumours within an age range of between 4 to 86 years. There was greater number of odontogenic tumours 417 (69.85%) than the bone related lesions 180 (30.15%). Of the odontogenic tumours, the epithetlial and in the bone related types, the fibro-osseous lesions were frequent. Conclusion: Ameloblastoma and ossifying fibroma were the most frequent tumours reported in this audit. The information regarding the prevalence of these tumours is scarce from the continent and can be useful in early detection and management before they cause facial deformity.展开更多
药物相关性颌骨坏死(medication-related osteonecrosis of the jaw,MRONJ)是指因恶性肿瘤骨转移、骨质疏松等疾病使用抗骨吸收类药物(anti-resorptive,AR)和抗血管生成类药物(anti-angiogenic,AA)等所致的颌骨代谢紊乱及骨坏死类疾病...药物相关性颌骨坏死(medication-related osteonecrosis of the jaw,MRONJ)是指因恶性肿瘤骨转移、骨质疏松等疾病使用抗骨吸收类药物(anti-resorptive,AR)和抗血管生成类药物(anti-angiogenic,AA)等所致的颌骨代谢紊乱及骨坏死类疾病。美国口腔颌面外科医师协会(American Association of Oral and Maxillofacial Surgeons,AAOMS)根据临床表现将MRONJ分成5期,其中,风险期指口服或静脉注射相关药物的患者,颌面部无任何异常症状;0期指没有发现临床可见的颌骨坏死,但是有非特异性的临床表现、影像学变化和症状。目前,0期MRONJ的诊断和治疗方法尚存在不确定性,如何有效预防风险期和0期患者向确立期的MRONJ转化存在争议。本文通过回顾0期MRONJ的诊断方法与治疗现状,分析针对风险期患者实施口腔有创操作的临床实践与研究成果,指出当前研究领域中存在的争议点与不确定性,旨在为未来的科学研究与临床实践提供参考与启示。展开更多
目的观察双侧卵巢切除术联合肌肉注射糖皮质激素对新西兰成年雌兔颌骨骨密度(bone mineral density,BMD)和生物力学的影响,探讨建立新西兰兔颌骨骨质疏松模型的方法。方法将15只5~6月龄新西兰成年雌兔按照随机分配法分为三组:双侧卵巢...目的观察双侧卵巢切除术联合肌肉注射糖皮质激素对新西兰成年雌兔颌骨骨密度(bone mineral density,BMD)和生物力学的影响,探讨建立新西兰兔颌骨骨质疏松模型的方法。方法将15只5~6月龄新西兰成年雌兔按照随机分配法分为三组:双侧卵巢切除术联合生理盐水肌肉注射组5只,为A组;双侧卵巢切除术联合糖皮质激素肌肉注射组5只,为B组;未切除双侧卵巢的假手术组联合生理盐水肌肉注射为对照组5只,为C组。术前及术后每月采用双能X射线骨密度仪进行兔颌骨骨密度测量,检测骨密度的变化。所有动物于手术3个月后检测颌骨生物力学的变化,进行组织染色病理学检查。结果术后3个月,B组新西兰兔下颌骨的BMD值下降29.59%(P<0.05),A组下降13.76%(P>0.05);B组在术后3个月时,下颌骨术后BMD均值小于术前BMD均值减去2SD;与C组相比,B组下颌骨的轴向最大压缩应力降低29.17%(P<0.05),A组下降4.17%(P>0.05);组织形态学观察B组相对于A组、C组,松质骨骨小梁变细,稀疏,数目变少,髓腔扩大,A组较C组变化不明显。结论双侧卵巢切除术联合糖皮质激素肌肉注射3个月可成功建立新西兰兔的颌骨骨质疏松模型。展开更多
目的探究超短波对颌骨骨髓间充质干细胞(JBMSCs)增殖、凋亡和成骨分化特性的影响。方法原代提取培养C57BL/6N小鼠JBMSCs并鉴定。取第三代JBMSCs进行实验,将细胞接种于培养板并随机分为非超短波处理组(Con)和超短波处理组(USW),超短波处...目的探究超短波对颌骨骨髓间充质干细胞(JBMSCs)增殖、凋亡和成骨分化特性的影响。方法原代提取培养C57BL/6N小鼠JBMSCs并鉴定。取第三代JBMSCs进行实验,将细胞接种于培养板并随机分为非超短波处理组(Con)和超短波处理组(USW),超短波处理组根据处理时长不同分为5组:30 s组、1 min组、3 min组、5 min组和7 min组,每天按照分组处理1次,通过CCK-8和qRT-PCR检测细胞增殖;Western blot、qRT-PCR检测凋亡相关标志物Bax、Bcl-2的表达,成骨相关标志物Runx2、ALP的表达。结果CCK-8结果显示,3 min USW处理JBMSCs可显著增强其第3天和第5天的OD值,3 min USW可上调Bcl-2的蛋白和mRNA表达水平,下调Bax的蛋白和mRNA表达水平;此外,3 min USW可有效上调ALP、Runx2的蛋白和mRNA表达水平。结论3 min USW处理能有效提高JBMSCs增殖和成骨分化能力并抑制其凋亡。展开更多
文摘Objective： To prospectively investigate the value of bone scintigraphy on determining the full extent of tumor involvement in jaw bones and to assess the presence of metastases. Methods： This study had local ethical committee approval, and all patients gave written informed consent. Thirty seven consecutive patients with primary malignant tumor in jaw bones were recruited for the study. Bone scintigraphy was performed in all patients before surgery to measure the full extent of bony involvement, which was compared with histologic findings. Results： Whole body scan revealed one case with multiple bony metastases. Resection specimens of 36 bone neoplasms were pathologically analyzed to identify type and size of each tumor. The lengths of the tumor involvement in jaw bones defined by bone scintigraphy and pathology were 5.62 ± 1.58 cm, 4.48 ± 1.57 cm, respectively （P 〈 0.05）. The tumor negative margins from removed specimens according to bone scintigraphy were pathologically confirmed. With histologic findings as the standard of reference, the accuracy of bone scintigraphy was 100% （36 of 36 patients） in determining the full extent of tumor involvement in jaw bones. Conclusion： Bone scintigraphy tends to offer specific guidelines in determining the appropriate extent of bone resection while entirely clearing the tumor cells and preserving functions whenever possible and in establishing the bony metastases.
文摘The growth and enlargement of jaw cysts are associated with raised intracystic pressure and bone resorption surrounding the cysts.The major bone-resorbing cells are the osteoclasts.They are acting under the influence of local bone-resorbing factors: prostaglandins,proteinases and cytokines.It was found that positive pressure enhanced the expression of IL-1αmRNA and protein in epithelial cells of odontogenic keratocyst,and increased the secretion of matrix metalloproteinase and PGE2 in a co-culture of odontogenic keratocyst fibroblasts and epithelial cells.However,the signal intensities for IL-1α mRNA and protein in the epithelium were significantly decreased after marsupialization which relived intracystic pressure.Experimental study indicated that intermittent negative pressure could promote osteogenesis in human bone marrow-derived stroma cells(BMSCs) in vitro.We propose a hypothesis that bone formation around the cyst of the jaws would be stimulated by intracystic negative pressure.
文摘A new method for filling bone cavity with a graft of frozen-decalcified-defatted-driedallogeneic (FDDDA) cancellous bone was described.This method was used for the treatment of cavi-ties after the enucleation of jaw cysts in 10 patients from December 1985 to February 1990.Thewounds of 9 patients healed by first intension,but wound infection occurred in one case postopera-tively.The 9 patients,besides the patient who suffered from wound infection,were followed up for5 to 56 months,with an average of 41.1 months.Evidence of recurrence with jaw cyst.was not ob-served.
基金a grant from National Natural Sciences Foundation of China (No. 30572065/ C03031103)
文摘To construct the recombinant adeno-associated virus （rAAV） vector with human bone morphogenetic protein 7 （BMP7） and observe the BMP7 mRNA expression in vitro, BMP7 CDS sequence was cloned into expression plasmid pAAV-MCS of AAV Helper Free System. The recombinant plasmid was identified with enzyme digestion and sequencing. The recombinant plasmid, pAAV-RC, pHelper were co-transfected into AAV-293 cells according to the calcium phosphate-based protocol. The viral stock was collected by 4 rounds of freeze/thaw. After purified and concentrated, the recombinant virus titer was determined by dot-blot assay. HEK293 cells were transfected with the recombinant virus at different MOI, and the expression of BMP7 mRNA was detected by RT-PCR. The results showed rAAV-BMP7 was constructed and packaged successfully. The physical particle titer was 2.5×10^11 vector genomes/mL. There was different expression level of BMP7 mRNA after transfecton. These data suggested that recombinant AAV mediated a stable expression of hBMP7 mRNA in 293 cells. The AAV production method may pave the way of an effective strategy for the jaw bone defection around dental implants.
文摘Background: The diversity of benign jaw tumours may cause difficulty in a correct diagnosis and insti-tution of an appropriate treatment. Data on the prevalence of these tumours is scarce from the Afri-can continent. We present a 19-year audit of benign jaw tumours and tumour-like lesions at a University teaching hospital in Nairobi, Kenya. Methods: Histo-pathological records were retrieved and re-examined from the Department of Oral and Maxillofacial pathology, University of Nairobi from 1992 to 2011. The jaw tumours were classified according to the latest WHO classification. Results: During the 19-year audit, 4257 biopsies were processed of which 597 (14.02%) were jaw tumours within an age range of between 4 to 86 years. There was greater number of odontogenic tumours 417 (69.85%) than the bone related lesions 180 (30.15%). Of the odontogenic tumours, the epithetlial and in the bone related types, the fibro-osseous lesions were frequent. Conclusion: Ameloblastoma and ossifying fibroma were the most frequent tumours reported in this audit. The information regarding the prevalence of these tumours is scarce from the continent and can be useful in early detection and management before they cause facial deformity.
文摘药物相关性颌骨坏死(medication-related osteonecrosis of the jaw,MRONJ)是指因恶性肿瘤骨转移、骨质疏松等疾病使用抗骨吸收类药物(anti-resorptive,AR)和抗血管生成类药物(anti-angiogenic,AA)等所致的颌骨代谢紊乱及骨坏死类疾病。美国口腔颌面外科医师协会(American Association of Oral and Maxillofacial Surgeons,AAOMS)根据临床表现将MRONJ分成5期,其中,风险期指口服或静脉注射相关药物的患者,颌面部无任何异常症状;0期指没有发现临床可见的颌骨坏死,但是有非特异性的临床表现、影像学变化和症状。目前,0期MRONJ的诊断和治疗方法尚存在不确定性,如何有效预防风险期和0期患者向确立期的MRONJ转化存在争议。本文通过回顾0期MRONJ的诊断方法与治疗现状,分析针对风险期患者实施口腔有创操作的临床实践与研究成果,指出当前研究领域中存在的争议点与不确定性,旨在为未来的科学研究与临床实践提供参考与启示。
文摘目的观察双侧卵巢切除术联合肌肉注射糖皮质激素对新西兰成年雌兔颌骨骨密度(bone mineral density,BMD)和生物力学的影响,探讨建立新西兰兔颌骨骨质疏松模型的方法。方法将15只5~6月龄新西兰成年雌兔按照随机分配法分为三组:双侧卵巢切除术联合生理盐水肌肉注射组5只,为A组;双侧卵巢切除术联合糖皮质激素肌肉注射组5只,为B组;未切除双侧卵巢的假手术组联合生理盐水肌肉注射为对照组5只,为C组。术前及术后每月采用双能X射线骨密度仪进行兔颌骨骨密度测量,检测骨密度的变化。所有动物于手术3个月后检测颌骨生物力学的变化,进行组织染色病理学检查。结果术后3个月,B组新西兰兔下颌骨的BMD值下降29.59%(P<0.05),A组下降13.76%(P>0.05);B组在术后3个月时,下颌骨术后BMD均值小于术前BMD均值减去2SD;与C组相比,B组下颌骨的轴向最大压缩应力降低29.17%(P<0.05),A组下降4.17%(P>0.05);组织形态学观察B组相对于A组、C组,松质骨骨小梁变细,稀疏,数目变少,髓腔扩大,A组较C组变化不明显。结论双侧卵巢切除术联合糖皮质激素肌肉注射3个月可成功建立新西兰兔的颌骨骨质疏松模型。
文摘目的探究超短波对颌骨骨髓间充质干细胞(JBMSCs)增殖、凋亡和成骨分化特性的影响。方法原代提取培养C57BL/6N小鼠JBMSCs并鉴定。取第三代JBMSCs进行实验,将细胞接种于培养板并随机分为非超短波处理组(Con)和超短波处理组(USW),超短波处理组根据处理时长不同分为5组:30 s组、1 min组、3 min组、5 min组和7 min组,每天按照分组处理1次,通过CCK-8和qRT-PCR检测细胞增殖;Western blot、qRT-PCR检测凋亡相关标志物Bax、Bcl-2的表达,成骨相关标志物Runx2、ALP的表达。结果CCK-8结果显示,3 min USW处理JBMSCs可显著增强其第3天和第5天的OD值,3 min USW可上调Bcl-2的蛋白和mRNA表达水平,下调Bax的蛋白和mRNA表达水平;此外,3 min USW可有效上调ALP、Runx2的蛋白和mRNA表达水平。结论3 min USW处理能有效提高JBMSCs增殖和成骨分化能力并抑制其凋亡。
