Genomic studies of Newcastle disease virus(NDV)affecting chickens and pigeons in Nigeria are limited.This study characterized NDVs from chickens and pigeons within households and live bird markets in Bauchi State,Nige...Genomic studies of Newcastle disease virus(NDV)affecting chickens and pigeons in Nigeria are limited.This study characterized NDVs from chickens and pigeons within households and live bird markets in Bauchi State,Nigeria.A total of 80 pooled oropharyngeal and cloacal swab samples were collected from November 2021 to February 2022 and tested using one-step RT-PCR.Interestingly,73(91.25%)of the samples were found to be positive,and 40 sequences of the partial NDV F gene were obtained.The fusion protein cleav-age site amino acid motifs 112R-R-Q/R-K-R-F117 and 112G-R-Q-G-R-L117 were observed for the virulent(95.0%)and lentogenic(5.0%)pathotypes,respectively.The virulent NDV strains clustered with subgenotype XIV.2 NDV strains from Nigeria and neighboring Benin,whereas the lentogenic pathotypes belonged to the genotype II cluster along with NDV vaccine strains.This study highlighted the se-rious threat of virulent NDV,warranting surveillance and improved biosecurity to control local and transboundary spread of NDV.展开更多
文摘Genomic studies of Newcastle disease virus(NDV)affecting chickens and pigeons in Nigeria are limited.This study characterized NDVs from chickens and pigeons within households and live bird markets in Bauchi State,Nigeria.A total of 80 pooled oropharyngeal and cloacal swab samples were collected from November 2021 to February 2022 and tested using one-step RT-PCR.Interestingly,73(91.25%)of the samples were found to be positive,and 40 sequences of the partial NDV F gene were obtained.The fusion protein cleav-age site amino acid motifs 112R-R-Q/R-K-R-F117 and 112G-R-Q-G-R-L117 were observed for the virulent(95.0%)and lentogenic(5.0%)pathotypes,respectively.The virulent NDV strains clustered with subgenotype XIV.2 NDV strains from Nigeria and neighboring Benin,whereas the lentogenic pathotypes belonged to the genotype II cluster along with NDV vaccine strains.This study highlighted the se-rious threat of virulent NDV,warranting surveillance and improved biosecurity to control local and transboundary spread of NDV.
