语境在口译理解、信息推理与预测、译语输出等方面都发挥着重要作用。数字化资源在口译教学中已经得到广泛应用,但语境缺失是其中不容忽视的问题。文章以技术手段重构资源语境为着眼点,以IVY(Interpreting in Virtual Reality,虚拟现实...语境在口译理解、信息推理与预测、译语输出等方面都发挥着重要作用。数字化资源在口译教学中已经得到广泛应用,但语境缺失是其中不容忽视的问题。文章以技术手段重构资源语境为着眼点,以IVY(Interpreting in Virtual Reality,虚拟现实口译)资源库为例,从环境语境、角色语境、认知语境、任务语境等方面探讨了数字化口译教学资源的语境重构方法,以期对提升数字化资源的应用价值、增强口译教学的效果有所助益。展开更多
Plant peroxidase extracted from Coccinia grandis Lin. （Ivy gourd） was purified from crude extract by ammonium sulfate precipitation, ion-exchange chromatography, and size exclusion chromatography. The purified enzym...Plant peroxidase extracted from Coccinia grandis Lin. （Ivy gourd） was purified from crude extract by ammonium sulfate precipitation, ion-exchange chromatography, and size exclusion chromatography. The purified enzyme preparation exhibited a specific activity of 6106.63 p.mol.minl.mg protein1, while purification fold and yield were 17.45 and 34.70%, respectively. The purified peroxidase was homogenous as judged by native and sodium dodecyl sulfate （SDS） polyacrylamide gel electrophoresis. The molecular weight as determined by gel filtration and SDS-polyacrylamide gel electrophoresis was 45 kD, which suggested that the purified peroxidase contained only one subunit. The apparent Km and Vmax values of the enzyme against phenol were 93 p.M and 561 μmol.min^-1.mg protein^-1, respectively. The temperature and pH optimum for purified peroxidase were 45℃ and pH 6.0, respective. However, it was stable at 30-60℃ and pH 4.0-8.0. The presence of metal ions such as Cu2＋ and Ca2＋ enhanced peroxidase activity. On the other hand, Cr3＋ and Hg2＋ strongly inhibited the enzyme activity at 500 p.M. Sodium dodecyl sulfate reduced a half of peroxidase activity at approximately 3 mM. Ivy gourd was stability in the presence of each urea concentrations. The affinity of the enzyme with different substrates showed as the highest relative activities on gallic acid followed by catechin, ascorbic acid and caffeic acid, respectively.展开更多
Recently,the press conference was held at Shanghai Time Square of Warner Hotel.Mrs Karin Malmstrom, Director of Cotton Council International, China was present at the conference and
文摘语境在口译理解、信息推理与预测、译语输出等方面都发挥着重要作用。数字化资源在口译教学中已经得到广泛应用,但语境缺失是其中不容忽视的问题。文章以技术手段重构资源语境为着眼点,以IVY(Interpreting in Virtual Reality,虚拟现实口译)资源库为例,从环境语境、角色语境、认知语境、任务语境等方面探讨了数字化口译教学资源的语境重构方法,以期对提升数字化资源的应用价值、增强口译教学的效果有所助益。
文摘Plant peroxidase extracted from Coccinia grandis Lin. （Ivy gourd） was purified from crude extract by ammonium sulfate precipitation, ion-exchange chromatography, and size exclusion chromatography. The purified enzyme preparation exhibited a specific activity of 6106.63 p.mol.minl.mg protein1, while purification fold and yield were 17.45 and 34.70%, respectively. The purified peroxidase was homogenous as judged by native and sodium dodecyl sulfate （SDS） polyacrylamide gel electrophoresis. The molecular weight as determined by gel filtration and SDS-polyacrylamide gel electrophoresis was 45 kD, which suggested that the purified peroxidase contained only one subunit. The apparent Km and Vmax values of the enzyme against phenol were 93 p.M and 561 μmol.min^-1.mg protein^-1, respectively. The temperature and pH optimum for purified peroxidase were 45℃ and pH 6.0, respective. However, it was stable at 30-60℃ and pH 4.0-8.0. The presence of metal ions such as Cu2＋ and Ca2＋ enhanced peroxidase activity. On the other hand, Cr3＋ and Hg2＋ strongly inhibited the enzyme activity at 500 p.M. Sodium dodecyl sulfate reduced a half of peroxidase activity at approximately 3 mM. Ivy gourd was stability in the presence of each urea concentrations. The affinity of the enzyme with different substrates showed as the highest relative activities on gallic acid followed by catechin, ascorbic acid and caffeic acid, respectively.
文摘Recently,the press conference was held at Shanghai Time Square of Warner Hotel.Mrs Karin Malmstrom, Director of Cotton Council International, China was present at the conference and
