Fish scaleδ^(15)N(δ^(15)N_(sca))records trophic information of the whole life history for marine fishes.Data from gut content and values calculated from white muscle tissue(WMT)are insufficient for assessing trophic...Fish scaleδ^(15)N(δ^(15)N_(sca))records trophic information of the whole life history for marine fishes.Data from gut content and values calculated from white muscle tissue(WMT)are insufficient for assessing trophic level(TL)of marine fishes as they only provide the information of several months or even several days.Stable isotope analysis(SIA)of nitrogen was carried out in fish scales and WMT of 16 species from a total of 28 individuals captured from the Yellow Sea and northern East China Sea in October and November of 2019,aiming at discovering the correlation betweenδ^(15)N_(sca) and fish WMTδ^(15)N(δ^(15)NWMT),so as to useδ^(15)N_(sca) for the characterization of TLs of marine fish.Scales were analyzed without prior carbonate dissolution as a pre-processing step.TL calculated fromδ^(15)N_(sca) was compared to traditional TL formula calculated withδ^(15)N_(WMT) to estimate the accuracy.We found that there was a highly significant and positive correlation(r=0.830,P<0.001)betweenδ^(15)N_(sca) andδ^(15)N_(WMT).TL calculation results using fish scale(TL_(sca))and WMT(TL_(WMT))showed congruence(r=0.773,P<0.001),and the regression coefficient betweenδ^(15)N_(sca) andδ^(15)NWMT was used for calculating TLsca.The conversion was conducted for the purpose of comparison with previousδ^(15)N_(WMT)-based calculation results of TL.TLsca calculation results were 81.25%within the TL range of±10%calculated withδ^(15)NWMT.The findings in the present study showed thatδ^(15)N_(sca) is a viable technique for the characterization of the TL of marine fish and can help to predict food web and support the study of marine ecosystem.展开更多
In the Early Ordovician Zhaogezhuang Section of Tangshan , North China, the Yeli Formation is composed of an entire third-order sequence, with facies ranging from the inner ramp restricted platform and open marine t...In the Early Ordovician Zhaogezhuang Section of Tangshan , North China, the Yeli Formation is composed of an entire third-order sequence, with facies ranging from the inner ramp restricted platform and open marine to the middle and even outer ramps. The Liangjiashan Formation is dominated by highstand system tracts （HST） with predominantly inner ramp grain-shoal and lagoon facies. Analyzing the carbon and oxygen isotope during the whole-rock carbonate reveals the 613C values in the Yeli Formation range from -7.11%o-0.76%o （PDB）, with the mean value at -2.98%0, while the 6180 values range of-9.09%o- -4.65%o with the mean value at -6.12%o. The 613C values in the Liangjiashan Formation range of -1.15%o-0.3%o, and the mean value of -0.57%0; the 61SO values are -8.76%0- -7.48%0, and the mean value is -8.06%o. The 613C values in the Yeli Formation decrease, but at the bottom of the Liangjiashan Formation the values increase steadily. In the middle-upper formation, there is an extended fluctuation between 0- -1.00%o. The 613C trend in the studied section is similar to that of the contemporary sections, except that it has much lower 613C values and a more negative excursion. The correlation between the 613C changes and the eustatic events, as well as the sedimentary facies, indicates that in the Tangshan area, the carbon isotope evolution can be attributabled to the processes of the eutrophic sea/oligotrophic sea, the seafloor organism- mediated oxidation in shallow water and the organic reduction after maximum flooding. The changes in the carbon isotope contents were primarily affected by the regional relative sea level changes. Compared to the other coeval data, the Early Ordovician of the Tang shan area is also severely depleted in 180, with all of the 6180sample values being Delow 5%o, except for one sample with a value ot -4.02%0. Witlt the ancient sea- water having a 6180 value of-5.5%0 （SMOW）, it is reasonable to delineate a temperature of less than 37 C.展开更多
Objective The Yangtze craton collisional orogeny at ca. extensional events at ca. 1 experienced Paleoproterozoic 1.95-2.0 Ga and post-orogenic 85 Ga related to amalgamation of the Columbia （Nuna） supercontinent （Zh...Objective The Yangtze craton collisional orogeny at ca. extensional events at ca. 1 experienced Paleoproterozoic 1.95-2.0 Ga and post-orogenic 85 Ga related to amalgamation of the Columbia （Nuna） supercontinent （Zhao and Cawood, 2012）. A ca. 2.15 Ga suprasubduction zone ophiolitic melange was recongized in the Archean- Paleoproterozoic Kongling Complex of the northern Yangtze craton （Han et al., 2017）. However, the tectonic evolution in early Paleoproterozoic from 2.4 Ga to 2.2 Ga remains unclear. We report here the presence of a suite of Paleoproterozoic （2.2 Ga） granites in the Huangling dome, northern Yangtze craton, which may provide important insights into crustal growth processes in the craton prior to the assembly of Columbia.展开更多
Freshwaterfishery resources in Chinese rivers have been markedly impaired as the results of overfishing,damming,and watershed development.However,little is known about trophic ecology offish assemblages in the rivers ...Freshwaterfishery resources in Chinese rivers have been markedly impaired as the results of overfishing,damming,and watershed development.However,little is known about trophic ecology offish assemblages in the rivers of China.In this study,fishes were collected from an upstream,midstream,and downstream site of the Moyangjiang River,Southern China,to investigate trophic niche structure using stable isotope analysis.We calculated several trophic niche metrics usingδ13C andδ15N ratios of thefish assemblages at each study site.Results showed no significant differences in theδ13C ratios between the pelagic and benthicfish assemblages at each study site.This suggests a homogenous dietary base shared by allfish at each site,likely as the result of continuous water column mixing in this shallow and fast-moving river.The upstreamfish assemblage had the greatest species abundance and trophic diversity.Thefish assemblage at the midstream site displayed the smallest trophic niche space(total area of theδ13C andδ15N bi-plot space)and trophic diversity.The midstream site also showed the most depleted 13C,suggesting a more degraded habitat compared with the other two study sites.Thefish assemblages at the downstream site displayed the greatest basal resource diversity,largest CR,and trophic niche space and trophic diversity.However,thefish assemblage at the downstream site also displayed the lowest species abundance,redundancy,and evenness of trophic spacing.Thesefindings suggest that the trophic niche of thefish assemblage at the downstream site,which received resources from upstreamflow,experienced greater watershed development and greater in situ production,demonstrating positive and negative impacts by anthropogenic activities.Future studies should also gather information onfish production,watershed development,and water quality,to aid in the interpretation of the stable isotope analysis offish trophic niches.展开更多
Revealing the integration between the nomadic pastoralists from the northern steppe and the agriculturalists in the Central Plains has always been key to exploring the formation and development of the Chinese national...Revealing the integration between the nomadic pastoralists from the northern steppe and the agriculturalists in the Central Plains has always been key to exploring the formation and development of the Chinese national community by the perspective of dietary cultural identity.In the Guanzhong region during the Sixteen Kingdoms period(304–439 AD),agricultural and nomadic populations coexisted,making it an important stage for the interaction of agricultural and nomadic dietary cultures.To date,there has still been a lack of direct evidence on revealing the integration of agricultural and nomadic populations in this region by the perspective of dietary cultural identity.Here,we analyzed 36 human bone samples from 25 individuals of the Sixteen Kingdoms period(including long bones and ribs from the same individual)from tombs of PhasesⅡandⅢof the Xi’an Xianyang Airport sites,conducting stable carbon and nitrogen isotope analysis.The results show that ancestors mainly consumed C_(4)-based(millets)foods or animals fed on their byproducts,supplemented by C_(3)-based(wheat,rice,etc.)foods,which reflects a subsistence strategy mixed with millet agriculture,wheat or rice agriculture,and livestock husbandry.The isotopic results of individuals uncovered from middle and high rank burials reflect the approval and reliance on the agricultural economy.Comparing the isotopic data of humans from the Han(202 BC–220 AD)to the Northern Wei dynasty(386–534 AD)in the Guanzhong region,as well as Xiongnu and Tuoba Xianbei in the steppe,we suggest that the nomads had already adopted and developed millet agriculture before migrating into Guanzhong region and that they fully adopted the agricultural economy during the Sixteen Kingdoms period.This indicates the more positive attitudes of the nomads to the agricultural culture,which promotes the integration of different populations and cultures ultimately.All in all,our study represents China’s first isotopic bioarchaeological research focusing on the Sixteen Kingdoms period,and provides an excellent case study for exploring dietary cultural identity and agro-pastoral integration in the Central Plains during this period.展开更多
Hydrocarbons are one of the important fluids within the Earth's crust,and different biotic and abitoic processes can generate hydrocarbon during geological periods.Tracing the sources and sinks of hydrocarbons can...Hydrocarbons are one of the important fluids within the Earth's crust,and different biotic and abitoic processes can generate hydrocarbon during geological periods.Tracing the sources and sinks of hydrocarbons can help us better understand the carbon cycle of the earth.In this study,an improved approach of adsorbed hydrocarbons extraction from sediments was established.The improved thermal desorption approach,compound-specific isotope analysis and position-specific isotope analysis were integrated to investigate the molecular and intramolecular isotope fractionation between trace hydrocarbon gases within sediments and geological hydrocarbon deposits.The isotopic compositions of the terminal position carbon of propane(δ^(13)C_(terminal))serves as a correlation indicator between trace hydrocarbon gases within sediments and geological hydrocarbon deposits.The tight sandstone gas from the Turpan-Hami Basin is a first case study for the application of this novel method to trace hydrocarbon origins.The results showed that the hydrocarbons in the tight sandstone gases in the study area most likely originated from humic organic matter(typeⅢkerogen)at an early mature stage.δ^(13)C_(terminal)values of the thermally desorbed propane gases from different source rocks were distinguishable and the values of the tight sandstone gases significantly overlap with those of the Lower Jurassic Sangonghe source rocks,suggesting their genetic relationship.Overall,the results provided novel position-specific carbon isotopic constraints on origins of hydrocarbons.展开更多
Mesozoic-Palaeozoic marine carbonate rocks are crucial hydrocarbon reservoirs in the Central Uplift area of the South Yellow Sea Basin(SYSB).Due to the scarcity of boreholes and the significant heterogeneity of carbon...Mesozoic-Palaeozoic marine carbonate rocks are crucial hydrocarbon reservoirs in the Central Uplift area of the South Yellow Sea Basin(SYSB).Due to the scarcity of boreholes and the significant heterogeneity of carbonate reservoirs,the distribution of porous carbonate reservoirs and their related key controlling factors remain unclear.In this study,factors affecting the distribution of porous Carboniferous-Early Permian carbonate reservoirs in the SYSB were investigated through seismic inversion and isotope analysis.The log-seismic characteristics of porous carbonate reservoirs,sensitive lithology parameters,and physical property parameters were extracted and analyzed.The pre-stack simultaneous inversion technique was applied to predict the lithology and physical properties of porous carbonate reservoirs.Moreover,the sedimentary of carbonate was analyzed using isotopes of carbon,oxygen,and strontium.The results show that porous carbonate reservoirs are mainly developed in the open platform sediments with porosities of 3%-5%and are mainly distributed in the paleo-highland(Huanglong Formation and Chuanshan Formation)and the slope of paleo-highland(Hezhou Formation).The porous carbonate reservoirs of the Qixia Formation are only locally developed.In addition,the negativeδ13C excursions indicate a warm and humid tropical climate with three sea-level fluctuations in the study area from the Carboniferous to Early Permian.The favorable conditions for developing porous carbonate rocks include the sedimentary environment and diagenetic process.The primary pore tends to form in high-energy environments of the paleo-highland,and the secondary pore is increased by dissolution during the syngenetic or quasi-syngenetic period.According to the hydrocarbon potential analysis,the Late Ordovician Wufeng Formation and Lower Silurian Gaojiabian Formation are the source rocks in the high-maturity-over-maturity stage,the Carboniferous-Lower Permian carbonate is the good reservoirs,and the Late Permian Longtan-Dalong Formation is the stable seal,ensuring a huge hydrocarbon accumulation potential in SYSB.The methods proposed in this study can be applied to other carbonate-dominated strata worldwide.展开更多
The growth trajectory of hailstones within clouds has remained elusive due to the inability to trace them directly,impeding the comprehension of their underlying growth mechanisms.This study investigated hailstone ver...The growth trajectory of hailstones within clouds has remained elusive due to the inability to trace them directly,impeding the comprehension of their underlying growth mechanisms.This study investigated hailstone vertical growth trajectories by detecting the stable isotope signatures(2H and 18O compositions)of different shells in 27 hailstones from 9hailstorms,which allowed us to capture the ambient temperature during hailstone growth.The vertical growth trajectories were obtained by comparing the isotopic compositions of water condensate in clouds,derived from the Adiabatic Model,with those measured in hailstones.Although hailstone growth was primarily observed in the–10°C to–30°C temperature layer,the embryo formation height and subsequent growth trajectories significantly varied among hailstones.Embryos formed over a wide range of temperatures(–8.7°C to–33.4°C);four originated at temperatures above–15°C and 16originated at temperatures below–20°C,suggesting ice nuclei composed of bioproteins and mineral dust,respectively.Among the 27 measured hailstones,3 exhibited minimal vertical movement,16 exhibited a monotonic rise or fall,and the remaining 8 exhibited alternating up-down trajectories;only one experienced“recycling”during up-down drifting.Trajectory analysis revealed that similar-sized hailstones from a single storm tended to form at similar heights,whereas those larger than 25 mm in diameter exhibited at least one period of upward growth.Vertical trajectories derived from isotopic analysis were corroborated by radar hydrometeor observations.展开更多
Isotope analysis has become an increasingly valuable tool in forensic anthropology case-work over the past decade. Modern-day isotopic investigations on human remains have integrated the use of multi-isotope profiles ...Isotope analysis has become an increasingly valuable tool in forensic anthropology case-work over the past decade. Modern-day isotopic investigations on human remains have integrated the use of multi-isotope profiles (e.g. C, N, O, H, S, Sr, and Pb) as well as iso-topic landscapes ("isoscapes") from multiple body tissues (e.g. teeth, bone, hair, and nails) to predict possible region-of-origin of unidentified human remains. Together, data from various isotope analyses provide additional lines of evidence for human identification, including a decedent's possible region-of-birth, long-term adult residence, recent travel history, and dietary choices. Here, we present the basic principles of isotope analysis and provide a brief overview of instrumentation, analytical standards, sample selection, and sample quality measures. Finally, we present case studies that reflect the diverse applications of isotope analysis to the medicolegal system before describing some future research directions. As shown herein, isotope analysis is a flexible and powerful geolocation tool that can provide new investigative leads for unidentified human remains cases.展开更多
The Beiqian site,located in Jimo city,Qingdao,went through three archaeological excavations,and a large number of human and animal bones in the Dawenkou culture and the Zhou Dynasty period were unearthed.By carbon and...The Beiqian site,located in Jimo city,Qingdao,went through three archaeological excavations,and a large number of human and animal bones in the Dawenkou culture and the Zhou Dynasty period were unearthed.By carbon and nitrogen stable isotope analysis,the diet of ancestors is expected to be recovered,and the domesticated plants and animals will be discussed.The investigated results showed that the food structure of ancestors in both periods included food crops,mainly C4 foods,and meat,mainly marine shellfish and livestock.For domestic pigs,they mainly eat C3 plants,and were affected by the lifestyle of ancestors.This study also showed that the diet of ancestors is the shellfish rather than marine fish despite the Beiqian site is located near the shoreline.We can conclude that the diet of ancestors in Dawenkou culture period included millet crops(C4plants),shellfish,marine food,and livestock,representing the lifestyle of farming,domestication,and fishing,supplemented by hunting.In the Zhou dynasty period the meat resource was enriched by domesticated animals and marine fisheries,and the living conditions were significantly improved.展开更多
Fish otolithδ^(15) N(δ^(15) N_(oto))is a demonstrated source of information of dietary history for marine fi sh as it is available iN_(oto)lith archives and sedimentary deposits unlike white muscle tissue(WMT).WMT a...Fish otolithδ^(15) N(δ^(15) N_(oto))is a demonstrated source of information of dietary history for marine fi sh as it is available iN_(oto)lith archives and sedimentary deposits unlike white muscle tissue(WMT).WMT and stomach content data are insufficient for trophic level(TL)data of past fi shes which is important for the changes of marine fi shery resources over long time scales.To determine the correlation betweenδ^(15) N_(oto) and fi sh WMTδ^(15) N(δ^(15) N_(wmt))and the feasibility of usingδ^(15) N_(oto) in characterizing the TLs of marine fi shes,we conducted nitrogen stable isotope analysis(SIA)in the otolith and WMT of 36 marine fi sh species sampled from the Yellow Sea and northern East China Sea in 2011-2014.Bothδ^(15) N_(oto) andδ^(15) N_(wmt) were analyzed using an elemental analyzer coupled with an isotope ratio mass spectrometer(EA-IRMS).Multiple otoliths were combined to make each otolith measurement and were analyzed as-is without a carbonate dissolution pre-processing step.δ^(15) N_(oto) andδ^(15) N_(wmt) comparisons for species in the Yellow Sea and northern East China Sea are currently lacking and would be helpful for both regional studies and for increasing the number of species for whichδ^(15) N_(oto) andδ^(15) N_(wmt) have been compared.Additionally,to determine the relative accuracy of trophic level calculated usingδ^(15) N_(oto),we compared TL calculated fromδ^(15) N_(oto) to traditional trophic level metrics calculated usingδ^(15) N_(wmt).The results showed a positive and highly signifi cant correlation(R=0.780,P<0.001)betweenδ^(15) N_(oto) andδ^(15) N_(wmt).Trophic level estimation using WMT(TL wmt)and otolith(TL oto)showed congruence in our study,which is not entirely surprising given thatδ^(15) N_(oto) was regressed againstδ^(15) N_(wmt) and the resulting regression coefficient was used to convertδ^(15) N_(oto) toδ^(15) N_(wmt) prior to calculating TL oto.This conversion was required in order to be consistent with previousδ^(15) N_(wmt)-based calculations of TL for comparison.TL oto calculations resulted in TL values that were largely within 5%-10%of TL values calculated withδ^(15) N_(wmt).Our fi ndings show thatδ^(15) N_(oto) is a feasible technique for characterizing the TLs of marine fi sh and can also assist in food web and marine ecosystem studies.展开更多
An accurate and sensitive method for the simultaneous determination of gibberellic acid（GA3）, gibberellin A4（GA4） and gibberellin A7（GA7） residues in tomato paste was developed by coupling solid phase extraction...An accurate and sensitive method for the simultaneous determination of gibberellic acid（GA3）, gibberellin A4（GA4） and gibberellin A7（GA7） residues in tomato paste was developed by coupling solid phase extraction to high performance liquid chromatography-tandem mass spectrometry（LC-MS/MS） with electrospray ionization based stable isotope dilution analysis（SIDA）. The isotope labeled internal standard can compensate for the losses during the extraction and cleanup steps and for discrimination due to ion suppression. After extraction from methanol, hydrophile lipophilic balance（HLB） solid phase extraction（SPE） column was tested for the capacity of the cleanup of the tomato paste in compared with C18 SPE column which is the common way to the detection of GAs, and the former gained better result. Spiked experiments were performed in the non-contaminated tomato pastes and the recoveries of GA3, GA4 and GA7 were 42.6%―75.0% in external standard method（ESM） and 91.1%―103.8% in internal standard method（ISM） respectively. The validities of this method were investigated and good analytical performance for the three GAs was obtained, including low limits of method detection（2 ng/g for GA3 and GA4, 0.3 ng/g for GA7）, excellent linear dynamic ranges（5―500 ng/g for GA3 and GA4, 1―100 ng/g for GA7） and good relative standard deviation ranges（4.8%―9.4% for the intra-day test and 3.5%―11.9% for the inter-day test）.展开更多
The nomenclature for compounds that are modified with isotopes is growing every day. Compounds can be modified with isotopes either individually, in a functional group or groups, or completely with all atomic centers ...The nomenclature for compounds that are modified with isotopes is growing every day. Compounds can be modified with isotopes either individually, in a functional group or groups, or completely with all atomic centers of the element. This diversity of isotope-modified compounds increases the range of researches that can be studied using them. Compounds modified with isotopes of carbon-13 or nitrogen-15 can be converted into carbon monoxide, carbon dioxide and molecular nitrogen. Currently, only the average value of carbon-13 or nitrogen-15 isotopes can be determined. However, by directly determining the atomic share of these isotopes in organic compounds modified with isotopes, information about the isotopic centers of the element can be obtained. The atomic fraction of an element is defined as a single carbon or nitrogen isotope-modified center or centers, or all centers that are isotope-modified with that element at the same time. Carbon-13 or nitrogen-15 isotopes’ atomic fraction can be determined molecularly or with fragment ions of different elemental content, or both. This makes the method self-verifying, increasing the accuracy and reliability of the results obtained. Amino acids, such as asparagine, aspartic acid, methionine, and threonine, are essential for the human body. This proposed method of isotopic analysis will increase the possibilities for scientific research using these compounds.展开更多
The article discusses a method for determining the atomic fraction of isotopes in boron trifluoride, boron carbide, boron anhydride, ferroboron and boric acid, by converting them into potassium tetrafluoroborate. This...The article discusses a method for determining the atomic fraction of isotopes in boron trifluoride, boron carbide, boron anhydride, ferroboron and boric acid, by converting them into potassium tetrafluoroborate. This compound is then analyzed using the spectrometric electron bombardment method. This technique is known for its high accuracy and “self-verifying” capabilities, meaning that the mass fraction of isotopes can be calculated using different mass lines, allowing for the identification of any potential errors.展开更多
In this study,using a dendrological and isotopic approaches,we investigated the responses to climate of two pure Fagus sylvatica L.stands(Campobraca and Falode)in the southernmost part of the distribution range in sou...In this study,using a dendrological and isotopic approaches,we investigated the responses to climate of two pure Fagus sylvatica L.stands(Campobraca and Falode)in the southernmost part of the distribution range in southern Italy.Theδ^(13)C data were used for calculating the intrinsic water use efficiency(iWUE)as a proxy of the balance between the water and carbon cycles.The results showed that the iWUE of both stands was sensitive to the amount of precipitation during the summer months(negative,significant effect)and to atmospheric CO_(2)concentration.Growth was sensitive to climate only in the Campo Braca site;the most influential variables were the VPD(vapour pressure deficit)and precipitation of the summer months that had a negative and a positive effect,respectively.The iWUE showed a negative correlation with growth in Campo Braca and a non-significant one in Falode.Water availability was the most influential variable on F.sylvatica growth and physiology.The iWUE increase was mainly driven by atmospheric CO_(2)concentration,and by decreased precipitation,as a response of the trees to drought.Our results highlight the importance of understanding the hydrological changes due to climate change for forecasting/modelling forest responses.CO_(2)increase does not compensate for the effect of adverse climate on F.sylvatica in the forests of southern Italy,while local conditions play an important role in determining tree growth.展开更多
The trophic ecology of the small yellow croaker （Larimichthys polyactis） was studied using stable isotope analyses. Samples were collected from July to September 2009 and 34 individuals from eight sites were examine...The trophic ecology of the small yellow croaker （Larimichthys polyactis） was studied using stable isotope analyses. Samples were collected from July to September 2009 and 34 individuals from eight sites were examined for stable carbon and nitrogen isotopes. Stable carbon isotope ratios （~3C） ranged from -20.67 to -15.43, while stable nitrogen isotope ratios （~SN） ranged 9.18-12.23. The relationship between fi^3C and ~SN suggested high resource partitioning in the sampling area. Significant differences in stable isotope values among the eight sampling sites may be linked to environmental diversities involving various physical processes （such as ocean current, wind and tide） and different carbon sources. Furthermore, the stable isotope ratios may also explain the ontogenetic variability in diet and feeding, because δ13C and δ15N varied significantly with increasing body size. The findings are consistent with other studies on diet analyses in small yellow croaker. It was also demonstrated that stable isotope analysis could be used to estimate the trophic characters of small yellow croaker in feeding patterns and migrating habits.展开更多
As apex predators, sharks are of ecological and conservation importance in marine ecosystems. In this study, trophic positions of sharks were estimated using stable isotope ratios of carbon and nitrogen for five repre...As apex predators, sharks are of ecological and conservation importance in marine ecosystems. In this study, trophic positions of sharks were estimated using stable isotope ratios of carbon and nitrogen for five representative species caught by the Chinese longline fleet in the mid-east Pacific, i.e., the blue shark （Prionace glauca）, the bigeye thresher shark （Alopias superciliosus）, the silky shark （Carcharhinus falciformis）, the scalloped hammerhead （Sphyrna lewini）, and the oceanic whitetip shark （Car-charhinus longimanus）. Of these species, oceanic whitetip shark has the lowest trophic level and mean 815N value （3.9 and 14.93%o± 0.84%o）, whereas bigeye thresher shark has the highest level/values （4.5 and 17.02%o±1.21%o, respectively）. The bigeye thresher shark has significantly higher 515N value than other shark species, indicating its higher trophic position. The blue shark and oceanic whitetip shark has significantly higher 813C values than bigeye thresher shark, silky shark and scalloped hammerhead, possibly due to different diets and/or living habitats. The stable isotope data and stomach content data are highly consistent, suggesting that sta-ble isotope analysis supplements traditional feeding ecology study of sharks, and thus contributes to understanding their trophic linkage.展开更多
This study aims to quantify the effects of different pretreatment methods on the stable carbon isotope values of fatty acids in marine microalgae（Isochrysis zhanjiangenisis）.To identify the effects of sample prepara...This study aims to quantify the effects of different pretreatment methods on the stable carbon isotope values of fatty acids in marine microalgae（Isochrysis zhanjiangenisis）.To identify the effects of sample preparation on theδ^（13）C value and the fatty acid composition,we examined eight types of pretreatment methods including：（a） drying the sample followed by direct methyl esterification using HCl-CH3OH;（b） drying the sample followed by direct methyl esterification using H2SO4-CH3OH;（c） drying the sample by ultrasonic extraction and methylesterification using HCl-CH3OH;（d） drying the sample by ultrasonic extraction and methyl-esterification using H2SO4-CH3OH;（e） fresh sample followed by direct methyl-esterification using HCl-CH3OH;（f） fresh sample followed by direct methyl-esterification using H2SO4-CH3OH;（g） fresh sample with ultrasonic extraction followed by methyl-esterification using HCl-CH3OH,and（h） fresh sample with ultrasonic extraction followed by methylesterification using H2SO4-CH3 OH.The results show that the δ^（13）C values from Groups a-e,g and h fluctuated within 0.3‰,and the δ^（13）C values of Group f were approximately 0.7‰ lower than the other seven groups.Therefore,the different sample pretreatment methods used towards the extraction of fatty acids from marine microalgae may result in different results regarding the stable carbon isotope ratios,and if necessary a correction should be applied.展开更多
As a new promising detection technology in the terahertz research field,the terahertz time-domain spec-troscopy(THz-TDS)has very broad application potential in many fields because its advantage on the characteristic s...As a new promising detection technology in the terahertz research field,the terahertz time-domain spec-troscopy(THz-TDS)has very broad application potential in many fields because its advantage on the characteristic spectrum,wide spectrum and non-destructive analysis of interested substances.In this paper,the terahertz absorption spectra of gases mixed with 12 CO and 13 CO in the spec-trum range of 0.5–2.5 THz are measured by terahertz time-domain spectroscopy for the first time.Several isotopo-logues can be clearly distinguished based on the difference in their rotational energies and the consequent terahertz spectrum.The experimental results show that 12 CO and 13 CO have obvious characteristic absorption peaks in the spectrum range of 0.5–2.5 THz due to the difference in rotational energy,and the rotational constant B can be calculated according to the experimental values to distin-guish the two gaseous isotopologues.The frequency posi-tions of the characteristic absorption peak measured by this experiment and the rotation constant B calculated accord-ing to the experimental values are compared with those previous theoretical calculations and experimental results,and they are in good agreement.This result lays a foun-dation for developing more sophisticated terahertz instru-ments to the detection of different isotopologues.展开更多
LA-MC-ICP-MS offers the ability to directly measure small variations in isotope composition at the micrometer scale in geological samples.However,when analyzing isotope ratios in complex minerals,mass spectrometric in...LA-MC-ICP-MS offers the ability to directly measure small variations in isotope composition at the micrometer scale in geological samples.However,when analyzing isotope ratios in complex minerals,mass spectrometric interferences from themselves and isotopic mass fractionation arising from the analytical process need to be carefully corrected.The lack of professional software to visualize and process raw data presents a significant challenge for all of LA-MC-ICP-MS labs.Moreover,the rapid development of instruments and the innovations in micro-analytical techniques necessitate specialized software to complete the complex correction and improve efficiency,such as for in situ isochron dating or new linear regression correction techniques.In this study,Plume,a free data processing software,was developed specifically for isotopic data processing in LA-MC-ICP-MS.The software provides a comprehensive set of functions,including baseline correction,signal selection and integration,mass spectrometric interference correction,isotopic fractionation correction,uncertainty propagation,delta value calculation,and real-time data processing.It also supports a complete correction workflow for the latest isochron dating using LA-(MC)-ICP-MS/MS,including isotopic and elemental ratios correction,the isochron drawing,and age calculation for multiple models.Additionally,a dedicated linear regression calculation unit has been developed in Plume to specialize in data processing of low signal-to-noise ratio or high spatial resolution analysis.These new features expand the application range of LA-MC-ICP-MS and improve work efficiency through batch processing.展开更多
基金supported by the Scientific Research Foundation of Hainan Tropical Ocean University(No.RHDRC202312)the Major Science and Technology Project of Yazhou Bay Innovation Institute of Hainan Tropical Ocean University:Research on Hainan Coastal Marine Ecological Environment and Carbon Sink(No.2022CXYZD002).
文摘Fish scaleδ^(15)N(δ^(15)N_(sca))records trophic information of the whole life history for marine fishes.Data from gut content and values calculated from white muscle tissue(WMT)are insufficient for assessing trophic level(TL)of marine fishes as they only provide the information of several months or even several days.Stable isotope analysis(SIA)of nitrogen was carried out in fish scales and WMT of 16 species from a total of 28 individuals captured from the Yellow Sea and northern East China Sea in October and November of 2019,aiming at discovering the correlation betweenδ^(15)N_(sca) and fish WMTδ^(15)N(δ^(15)NWMT),so as to useδ^(15)N_(sca) for the characterization of TLs of marine fish.Scales were analyzed without prior carbonate dissolution as a pre-processing step.TL calculated fromδ^(15)N_(sca) was compared to traditional TL formula calculated withδ^(15)N_(WMT) to estimate the accuracy.We found that there was a highly significant and positive correlation(r=0.830,P<0.001)betweenδ^(15)N_(sca) andδ^(15)N_(WMT).TL calculation results using fish scale(TL_(sca))and WMT(TL_(WMT))showed congruence(r=0.773,P<0.001),and the regression coefficient betweenδ^(15)N_(sca) andδ^(15)NWMT was used for calculating TLsca.The conversion was conducted for the purpose of comparison with previousδ^(15)N_(WMT)-based calculation results of TL.TLsca calculation results were 81.25%within the TL range of±10%calculated withδ^(15)NWMT.The findings in the present study showed thatδ^(15)N_(sca) is a viable technique for the characterization of the TL of marine fish and can help to predict food web and support the study of marine ecosystem.
基金supported by the National Natural Science Foundation of China(No.41372039)
文摘In the Early Ordovician Zhaogezhuang Section of Tangshan , North China, the Yeli Formation is composed of an entire third-order sequence, with facies ranging from the inner ramp restricted platform and open marine to the middle and even outer ramps. The Liangjiashan Formation is dominated by highstand system tracts （HST） with predominantly inner ramp grain-shoal and lagoon facies. Analyzing the carbon and oxygen isotope during the whole-rock carbonate reveals the 613C values in the Yeli Formation range from -7.11%o-0.76%o （PDB）, with the mean value at -2.98%0, while the 6180 values range of-9.09%o- -4.65%o with the mean value at -6.12%o. The 613C values in the Liangjiashan Formation range of -1.15%o-0.3%o, and the mean value of -0.57%0; the 61SO values are -8.76%0- -7.48%0, and the mean value is -8.06%o. The 613C values in the Yeli Formation decrease, but at the bottom of the Liangjiashan Formation the values increase steadily. In the middle-upper formation, there is an extended fluctuation between 0- -1.00%o. The 613C trend in the studied section is similar to that of the contemporary sections, except that it has much lower 613C values and a more negative excursion. The correlation between the 613C changes and the eustatic events, as well as the sedimentary facies, indicates that in the Tangshan area, the carbon isotope evolution can be attributabled to the processes of the eutrophic sea/oligotrophic sea, the seafloor organism- mediated oxidation in shallow water and the organic reduction after maximum flooding. The changes in the carbon isotope contents were primarily affected by the regional relative sea level changes. Compared to the other coeval data, the Early Ordovician of the Tang shan area is also severely depleted in 180, with all of the 6180sample values being Delow 5%o, except for one sample with a value ot -4.02%0. Witlt the ancient sea- water having a 6180 value of-5.5%0 （SMOW）, it is reasonable to delineate a temperature of less than 37 C.
基金supported by the funded project of the China Geological Survey(grants No.12120113061700,121201009000150013 and DD20160029)
文摘Objective The Yangtze craton collisional orogeny at ca. extensional events at ca. 1 experienced Paleoproterozoic 1.95-2.0 Ga and post-orogenic 85 Ga related to amalgamation of the Columbia （Nuna） supercontinent （Zhao and Cawood, 2012）. A ca. 2.15 Ga suprasubduction zone ophiolitic melange was recongized in the Archean- Paleoproterozoic Kongling Complex of the northern Yangtze craton （Han et al., 2017）. However, the tectonic evolution in early Paleoproterozoic from 2.4 Ga to 2.2 Ga remains unclear. We report here the presence of a suite of Paleoproterozoic （2.2 Ga） granites in the Huangling dome, northern Yangtze craton, which may provide important insights into crustal growth processes in the craton prior to the assembly of Columbia.
基金Natural National Science Foundation of China,Grant/Award Number:41376158Bureau of Science and Technology of Guangdong Province,Grant/Award Numbers:2005A20105001,2006B20701001,B200601G02China Agriculture System,Grant/Award Number:CARS-50。
文摘Freshwaterfishery resources in Chinese rivers have been markedly impaired as the results of overfishing,damming,and watershed development.However,little is known about trophic ecology offish assemblages in the rivers of China.In this study,fishes were collected from an upstream,midstream,and downstream site of the Moyangjiang River,Southern China,to investigate trophic niche structure using stable isotope analysis.We calculated several trophic niche metrics usingδ13C andδ15N ratios of thefish assemblages at each study site.Results showed no significant differences in theδ13C ratios between the pelagic and benthicfish assemblages at each study site.This suggests a homogenous dietary base shared by allfish at each site,likely as the result of continuous water column mixing in this shallow and fast-moving river.The upstreamfish assemblage had the greatest species abundance and trophic diversity.Thefish assemblage at the midstream site displayed the smallest trophic niche space(total area of theδ13C andδ15N bi-plot space)and trophic diversity.The midstream site also showed the most depleted 13C,suggesting a more degraded habitat compared with the other two study sites.Thefish assemblages at the downstream site displayed the greatest basal resource diversity,largest CR,and trophic niche space and trophic diversity.However,thefish assemblage at the downstream site also displayed the lowest species abundance,redundancy,and evenness of trophic spacing.Thesefindings suggest that the trophic niche of thefish assemblage at the downstream site,which received resources from upstreamflow,experienced greater watershed development and greater in situ production,demonstrating positive and negative impacts by anthropogenic activities.Future studies should also gather information onfish production,watershed development,and water quality,to aid in the interpretation of the stable isotope analysis offish trophic niches.
基金supported by the National Social Science Fund of China(Grant No.21&ZD237)the National Natural Science Foundation of China(Grant No.421172007)。
文摘Revealing the integration between the nomadic pastoralists from the northern steppe and the agriculturalists in the Central Plains has always been key to exploring the formation and development of the Chinese national community by the perspective of dietary cultural identity.In the Guanzhong region during the Sixteen Kingdoms period(304–439 AD),agricultural and nomadic populations coexisted,making it an important stage for the interaction of agricultural and nomadic dietary cultures.To date,there has still been a lack of direct evidence on revealing the integration of agricultural and nomadic populations in this region by the perspective of dietary cultural identity.Here,we analyzed 36 human bone samples from 25 individuals of the Sixteen Kingdoms period(including long bones and ribs from the same individual)from tombs of PhasesⅡandⅢof the Xi’an Xianyang Airport sites,conducting stable carbon and nitrogen isotope analysis.The results show that ancestors mainly consumed C_(4)-based(millets)foods or animals fed on their byproducts,supplemented by C_(3)-based(wheat,rice,etc.)foods,which reflects a subsistence strategy mixed with millet agriculture,wheat or rice agriculture,and livestock husbandry.The isotopic results of individuals uncovered from middle and high rank burials reflect the approval and reliance on the agricultural economy.Comparing the isotopic data of humans from the Han(202 BC–220 AD)to the Northern Wei dynasty(386–534 AD)in the Guanzhong region,as well as Xiongnu and Tuoba Xianbei in the steppe,we suggest that the nomads had already adopted and developed millet agriculture before migrating into Guanzhong region and that they fully adopted the agricultural economy during the Sixteen Kingdoms period.This indicates the more positive attitudes of the nomads to the agricultural culture,which promotes the integration of different populations and cultures ultimately.All in all,our study represents China’s first isotopic bioarchaeological research focusing on the Sixteen Kingdoms period,and provides an excellent case study for exploring dietary cultural identity and agro-pastoral integration in the Central Plains during this period.
基金financially supported by the National Natural Science Foundation of China(Grant No.42102202)U.S.Department of Energy Geosciences program(DE-SC0016271)。
文摘Hydrocarbons are one of the important fluids within the Earth's crust,and different biotic and abitoic processes can generate hydrocarbon during geological periods.Tracing the sources and sinks of hydrocarbons can help us better understand the carbon cycle of the earth.In this study,an improved approach of adsorbed hydrocarbons extraction from sediments was established.The improved thermal desorption approach,compound-specific isotope analysis and position-specific isotope analysis were integrated to investigate the molecular and intramolecular isotope fractionation between trace hydrocarbon gases within sediments and geological hydrocarbon deposits.The isotopic compositions of the terminal position carbon of propane(δ^(13)C_(terminal))serves as a correlation indicator between trace hydrocarbon gases within sediments and geological hydrocarbon deposits.The tight sandstone gas from the Turpan-Hami Basin is a first case study for the application of this novel method to trace hydrocarbon origins.The results showed that the hydrocarbons in the tight sandstone gases in the study area most likely originated from humic organic matter(typeⅢkerogen)at an early mature stage.δ^(13)C_(terminal)values of the thermally desorbed propane gases from different source rocks were distinguishable and the values of the tight sandstone gases significantly overlap with those of the Lower Jurassic Sangonghe source rocks,suggesting their genetic relationship.Overall,the results provided novel position-specific carbon isotopic constraints on origins of hydrocarbons.
基金This study was supported by the project ofthe Science and Technology Innovation Fund of Command Center of Natural Resources Intergrated Survey entitled“Temporal and spatial distribution of paleochannel and origin of organic carbon burial in the Western Bohai Sea since 2.28Ma”(KC20220011)the project entitled“Characterization of Carboniferous-Early Permian heterogeneous porous carbonate reservoirs and hydrocarbon potential analysis in the central uplift of the South Yellow Sea Basin”(KLSG2304)+3 种基金by the Key laboratory of Submarine Science,Ministry of Natural Resources,the project entitled“1∶50000 Marine regional Geological survey in Caofeidian Sea Area,Bohai Sea”(ZD20220602)“1∶250000 Marine regional Geological survey in Weihai Sea Area,North Yellow Sea”(DD20230412)“Geological survey on tectonic and sedimentary conditions of Laoshan uplift”(DD2016015)by the China Geological Survey,and the project entitled“Study on Hydrocarbon Accumulation Failure and Fluid Evolution Reduction of the Permian Reservoir in the Laoshan Uplift,South Yellow Sea”(42076220)organized by the National Natural Science Foundation of China.
文摘Mesozoic-Palaeozoic marine carbonate rocks are crucial hydrocarbon reservoirs in the Central Uplift area of the South Yellow Sea Basin(SYSB).Due to the scarcity of boreholes and the significant heterogeneity of carbonate reservoirs,the distribution of porous carbonate reservoirs and their related key controlling factors remain unclear.In this study,factors affecting the distribution of porous Carboniferous-Early Permian carbonate reservoirs in the SYSB were investigated through seismic inversion and isotope analysis.The log-seismic characteristics of porous carbonate reservoirs,sensitive lithology parameters,and physical property parameters were extracted and analyzed.The pre-stack simultaneous inversion technique was applied to predict the lithology and physical properties of porous carbonate reservoirs.Moreover,the sedimentary of carbonate was analyzed using isotopes of carbon,oxygen,and strontium.The results show that porous carbonate reservoirs are mainly developed in the open platform sediments with porosities of 3%-5%and are mainly distributed in the paleo-highland(Huanglong Formation and Chuanshan Formation)and the slope of paleo-highland(Hezhou Formation).The porous carbonate reservoirs of the Qixia Formation are only locally developed.In addition,the negativeδ13C excursions indicate a warm and humid tropical climate with three sea-level fluctuations in the study area from the Carboniferous to Early Permian.The favorable conditions for developing porous carbonate rocks include the sedimentary environment and diagenetic process.The primary pore tends to form in high-energy environments of the paleo-highland,and the secondary pore is increased by dissolution during the syngenetic or quasi-syngenetic period.According to the hydrocarbon potential analysis,the Late Ordovician Wufeng Formation and Lower Silurian Gaojiabian Formation are the source rocks in the high-maturity-over-maturity stage,the Carboniferous-Lower Permian carbonate is the good reservoirs,and the Late Permian Longtan-Dalong Formation is the stable seal,ensuring a huge hydrocarbon accumulation potential in SYSB.The methods proposed in this study can be applied to other carbonate-dominated strata worldwide.
基金supported by the National Natural Science Foundation of China(Grant No.42030607)the Beijing Municipal Science and Technology Commission(Grant No.Z251100004525005)the National Science Foundation/National Center for Atmospheric Research,and NASA(Grant No.80NSSC22M0129)。
文摘The growth trajectory of hailstones within clouds has remained elusive due to the inability to trace them directly,impeding the comprehension of their underlying growth mechanisms.This study investigated hailstone vertical growth trajectories by detecting the stable isotope signatures(2H and 18O compositions)of different shells in 27 hailstones from 9hailstorms,which allowed us to capture the ambient temperature during hailstone growth.The vertical growth trajectories were obtained by comparing the isotopic compositions of water condensate in clouds,derived from the Adiabatic Model,with those measured in hailstones.Although hailstone growth was primarily observed in the–10°C to–30°C temperature layer,the embryo formation height and subsequent growth trajectories significantly varied among hailstones.Embryos formed over a wide range of temperatures(–8.7°C to–33.4°C);four originated at temperatures above–15°C and 16originated at temperatures below–20°C,suggesting ice nuclei composed of bioproteins and mineral dust,respectively.Among the 27 measured hailstones,3 exhibited minimal vertical movement,16 exhibited a monotonic rise or fall,and the remaining 8 exhibited alternating up-down trajectories;only one experienced“recycling”during up-down drifting.Trajectory analysis revealed that similar-sized hailstones from a single storm tended to form at similar heights,whereas those larger than 25 mm in diameter exhibited at least one period of upward growth.Vertical trajectories derived from isotopic analysis were corroborated by radar hydrometeor observations.
文摘Isotope analysis has become an increasingly valuable tool in forensic anthropology case-work over the past decade. Modern-day isotopic investigations on human remains have integrated the use of multi-isotope profiles (e.g. C, N, O, H, S, Sr, and Pb) as well as iso-topic landscapes ("isoscapes") from multiple body tissues (e.g. teeth, bone, hair, and nails) to predict possible region-of-origin of unidentified human remains. Together, data from various isotope analyses provide additional lines of evidence for human identification, including a decedent's possible region-of-birth, long-term adult residence, recent travel history, and dietary choices. Here, we present the basic principles of isotope analysis and provide a brief overview of instrumentation, analytical standards, sample selection, and sample quality measures. Finally, we present case studies that reflect the diverse applications of isotope analysis to the medicolegal system before describing some future research directions. As shown herein, isotope analysis is a flexible and powerful geolocation tool that can provide new investigative leads for unidentified human remains cases.
基金supported by the National Social Science Fund(Grant Nos.11CKG004 and 11AZD116)Natural Science Foundation of Shandong Province(Grant No.ZR2012BQ025)Innovation Fund of Shandong University(Grant No.IFYT12038)
文摘The Beiqian site,located in Jimo city,Qingdao,went through three archaeological excavations,and a large number of human and animal bones in the Dawenkou culture and the Zhou Dynasty period were unearthed.By carbon and nitrogen stable isotope analysis,the diet of ancestors is expected to be recovered,and the domesticated plants and animals will be discussed.The investigated results showed that the food structure of ancestors in both periods included food crops,mainly C4 foods,and meat,mainly marine shellfish and livestock.For domestic pigs,they mainly eat C3 plants,and were affected by the lifestyle of ancestors.This study also showed that the diet of ancestors is the shellfish rather than marine fish despite the Beiqian site is located near the shoreline.We can conclude that the diet of ancestors in Dawenkou culture period included millet crops(C4plants),shellfish,marine food,and livestock,representing the lifestyle of farming,domestication,and fishing,supplemented by hunting.In the Zhou dynasty period the meat resource was enriched by domesticated animals and marine fisheries,and the living conditions were significantly improved.
基金Supported by the National Key Basic Research Development Program of China(973 Program)(No.2015CB453303)the National Science Foundation for Young Scientists of China(No.41606195)。
文摘Fish otolithδ^(15) N(δ^(15) N_(oto))is a demonstrated source of information of dietary history for marine fi sh as it is available iN_(oto)lith archives and sedimentary deposits unlike white muscle tissue(WMT).WMT and stomach content data are insufficient for trophic level(TL)data of past fi shes which is important for the changes of marine fi shery resources over long time scales.To determine the correlation betweenδ^(15) N_(oto) and fi sh WMTδ^(15) N(δ^(15) N_(wmt))and the feasibility of usingδ^(15) N_(oto) in characterizing the TLs of marine fi shes,we conducted nitrogen stable isotope analysis(SIA)in the otolith and WMT of 36 marine fi sh species sampled from the Yellow Sea and northern East China Sea in 2011-2014.Bothδ^(15) N_(oto) andδ^(15) N_(wmt) were analyzed using an elemental analyzer coupled with an isotope ratio mass spectrometer(EA-IRMS).Multiple otoliths were combined to make each otolith measurement and were analyzed as-is without a carbonate dissolution pre-processing step.δ^(15) N_(oto) andδ^(15) N_(wmt) comparisons for species in the Yellow Sea and northern East China Sea are currently lacking and would be helpful for both regional studies and for increasing the number of species for whichδ^(15) N_(oto) andδ^(15) N_(wmt) have been compared.Additionally,to determine the relative accuracy of trophic level calculated usingδ^(15) N_(oto),we compared TL calculated fromδ^(15) N_(oto) to traditional trophic level metrics calculated usingδ^(15) N_(wmt).The results showed a positive and highly signifi cant correlation(R=0.780,P<0.001)betweenδ^(15) N_(oto) andδ^(15) N_(wmt).Trophic level estimation using WMT(TL wmt)and otolith(TL oto)showed congruence in our study,which is not entirely surprising given thatδ^(15) N_(oto) was regressed againstδ^(15) N_(wmt) and the resulting regression coefficient was used to convertδ^(15) N_(oto) toδ^(15) N_(wmt) prior to calculating TL oto.This conversion was required in order to be consistent with previousδ^(15) N_(wmt)-based calculations of TL for comparison.TL oto calculations resulted in TL values that were largely within 5%-10%of TL values calculated withδ^(15) N_(wmt).Our fi ndings show thatδ^(15) N_(oto) is a feasible technique for characterizing the TLs of marine fi sh and can also assist in food web and marine ecosystem studies.
基金Supported by the Project of Chinese Academy of Inspection and Quanrantine(No.2010JK001)the Project of Xinjiang Entry-exit Inspection and Quarantine Bureau, China(No.2010XK0033)
文摘An accurate and sensitive method for the simultaneous determination of gibberellic acid（GA3）, gibberellin A4（GA4） and gibberellin A7（GA7） residues in tomato paste was developed by coupling solid phase extraction to high performance liquid chromatography-tandem mass spectrometry（LC-MS/MS） with electrospray ionization based stable isotope dilution analysis（SIDA）. The isotope labeled internal standard can compensate for the losses during the extraction and cleanup steps and for discrimination due to ion suppression. After extraction from methanol, hydrophile lipophilic balance（HLB） solid phase extraction（SPE） column was tested for the capacity of the cleanup of the tomato paste in compared with C18 SPE column which is the common way to the detection of GAs, and the former gained better result. Spiked experiments were performed in the non-contaminated tomato pastes and the recoveries of GA3, GA4 and GA7 were 42.6%―75.0% in external standard method（ESM） and 91.1%―103.8% in internal standard method（ISM） respectively. The validities of this method were investigated and good analytical performance for the three GAs was obtained, including low limits of method detection（2 ng/g for GA3 and GA4, 0.3 ng/g for GA7）, excellent linear dynamic ranges（5―500 ng/g for GA3 and GA4, 1―100 ng/g for GA7） and good relative standard deviation ranges（4.8%―9.4% for the intra-day test and 3.5%―11.9% for the inter-day test）.
文摘The nomenclature for compounds that are modified with isotopes is growing every day. Compounds can be modified with isotopes either individually, in a functional group or groups, or completely with all atomic centers of the element. This diversity of isotope-modified compounds increases the range of researches that can be studied using them. Compounds modified with isotopes of carbon-13 or nitrogen-15 can be converted into carbon monoxide, carbon dioxide and molecular nitrogen. Currently, only the average value of carbon-13 or nitrogen-15 isotopes can be determined. However, by directly determining the atomic share of these isotopes in organic compounds modified with isotopes, information about the isotopic centers of the element can be obtained. The atomic fraction of an element is defined as a single carbon or nitrogen isotope-modified center or centers, or all centers that are isotope-modified with that element at the same time. Carbon-13 or nitrogen-15 isotopes’ atomic fraction can be determined molecularly or with fragment ions of different elemental content, or both. This makes the method self-verifying, increasing the accuracy and reliability of the results obtained. Amino acids, such as asparagine, aspartic acid, methionine, and threonine, are essential for the human body. This proposed method of isotopic analysis will increase the possibilities for scientific research using these compounds.
文摘The article discusses a method for determining the atomic fraction of isotopes in boron trifluoride, boron carbide, boron anhydride, ferroboron and boric acid, by converting them into potassium tetrafluoroborate. This compound is then analyzed using the spectrometric electron bombardment method. This technique is known for its high accuracy and “self-verifying” capabilities, meaning that the mass fraction of isotopes can be calculated using different mass lines, allowing for the identification of any potential errors.
基金partially supported by MIUR Project (PRIN 2020) "Unravelling interactions between WATER and carbon cycles during drought and their impact on water resources and forest and grassland ecosySTEMs in the Mediterranean climate (WATERSTEM)" (protocol code:20202WF53Z)"WAFER" at CNR (Consiglio Nazionale delle Ricerche)MIUR Project (PRIN 2017) "The Italian TREETALKER NETWORK:continuous large scale monitoring of tree functional traits and vulnerabilities to climate change"
文摘In this study,using a dendrological and isotopic approaches,we investigated the responses to climate of two pure Fagus sylvatica L.stands(Campobraca and Falode)in the southernmost part of the distribution range in southern Italy.Theδ^(13)C data were used for calculating the intrinsic water use efficiency(iWUE)as a proxy of the balance between the water and carbon cycles.The results showed that the iWUE of both stands was sensitive to the amount of precipitation during the summer months(negative,significant effect)and to atmospheric CO_(2)concentration.Growth was sensitive to climate only in the Campo Braca site;the most influential variables were the VPD(vapour pressure deficit)and precipitation of the summer months that had a negative and a positive effect,respectively.The iWUE showed a negative correlation with growth in Campo Braca and a non-significant one in Falode.Water availability was the most influential variable on F.sylvatica growth and physiology.The iWUE increase was mainly driven by atmospheric CO_(2)concentration,and by decreased precipitation,as a response of the trees to drought.Our results highlight the importance of understanding the hydrological changes due to climate change for forecasting/modelling forest responses.CO_(2)increase does not compensate for the effect of adverse climate on F.sylvatica in the forests of southern Italy,while local conditions play an important role in determining tree growth.
基金Supported by the Special Research Fund for the National Non-profit in East China Sea Fisheries Research Institute (No. 2009M01)
文摘The trophic ecology of the small yellow croaker （Larimichthys polyactis） was studied using stable isotope analyses. Samples were collected from July to September 2009 and 34 individuals from eight sites were examined for stable carbon and nitrogen isotopes. Stable carbon isotope ratios （~3C） ranged from -20.67 to -15.43, while stable nitrogen isotope ratios （~SN） ranged 9.18-12.23. The relationship between fi^3C and ~SN suggested high resource partitioning in the sampling area. Significant differences in stable isotope values among the eight sampling sites may be linked to environmental diversities involving various physical processes （such as ocean current, wind and tide） and different carbon sources. Furthermore, the stable isotope ratios may also explain the ontogenetic variability in diet and feeding, because δ13C and δ15N varied significantly with increasing body size. The findings are consistent with other studies on diet analyses in small yellow croaker. It was also demonstrated that stable isotope analysis could be used to estimate the trophic characters of small yellow croaker in feeding patterns and migrating habits.
基金Li Yunkai was supported by the National Natural Science Foundation of China (No.41206124)Ph.D.Programs Foundation of Ministry of Education of China (No.201 23104120001)+3 种基金the ‘Chen Guang’ Project of Shanghai Municipal Education Commission (No.D8004-10-0206)the Shanghai Education Development Foundation (No.B-8102-10-0084)Zhu Jiangfeng and Dai Xiaojie were supported by the National Natural Science Foundation of China (No.41106118)the Scientific Observing and Experimental Station of Oceanic Fishery Resources, Ministry of Agriculture, China
文摘As apex predators, sharks are of ecological and conservation importance in marine ecosystems. In this study, trophic positions of sharks were estimated using stable isotope ratios of carbon and nitrogen for five representative species caught by the Chinese longline fleet in the mid-east Pacific, i.e., the blue shark （Prionace glauca）, the bigeye thresher shark （Alopias superciliosus）, the silky shark （Carcharhinus falciformis）, the scalloped hammerhead （Sphyrna lewini）, and the oceanic whitetip shark （Car-charhinus longimanus）. Of these species, oceanic whitetip shark has the lowest trophic level and mean 815N value （3.9 and 14.93%o± 0.84%o）, whereas bigeye thresher shark has the highest level/values （4.5 and 17.02%o±1.21%o, respectively）. The bigeye thresher shark has significantly higher 515N value than other shark species, indicating its higher trophic position. The blue shark and oceanic whitetip shark has significantly higher 813C values than bigeye thresher shark, silky shark and scalloped hammerhead, possibly due to different diets and/or living habitats. The stable isotope data and stomach content data are highly consistent, suggesting that sta-ble isotope analysis supplements traditional feeding ecology study of sharks, and thus contributes to understanding their trophic linkage.
基金The State Oceanic Public Welfare Project of China under contract Nos 2012418012-02 and 201305002
文摘This study aims to quantify the effects of different pretreatment methods on the stable carbon isotope values of fatty acids in marine microalgae（Isochrysis zhanjiangenisis）.To identify the effects of sample preparation on theδ^（13）C value and the fatty acid composition,we examined eight types of pretreatment methods including：（a） drying the sample followed by direct methyl esterification using HCl-CH3OH;（b） drying the sample followed by direct methyl esterification using H2SO4-CH3OH;（c） drying the sample by ultrasonic extraction and methylesterification using HCl-CH3OH;（d） drying the sample by ultrasonic extraction and methyl-esterification using H2SO4-CH3OH;（e） fresh sample followed by direct methyl-esterification using HCl-CH3OH;（f） fresh sample followed by direct methyl-esterification using H2SO4-CH3OH;（g） fresh sample with ultrasonic extraction followed by methyl-esterification using HCl-CH3OH,and（h） fresh sample with ultrasonic extraction followed by methylesterification using H2SO4-CH3 OH.The results show that the δ^（13）C values from Groups a-e,g and h fluctuated within 0.3‰,and the δ^（13）C values of Group f were approximately 0.7‰ lower than the other seven groups.Therefore,the different sample pretreatment methods used towards the extraction of fatty acids from marine microalgae may result in different results regarding the stable carbon isotope ratios,and if necessary a correction should be applied.
基金supported by Chinese NSF project(42130114)the strategic priority research program(B)of CAS(XDB41000000)the pre-research Project on Civil Aerospace Technologies No.D020202 funded by Chinese National Space Administration(CNSA).
文摘As a new promising detection technology in the terahertz research field,the terahertz time-domain spec-troscopy(THz-TDS)has very broad application potential in many fields because its advantage on the characteristic spectrum,wide spectrum and non-destructive analysis of interested substances.In this paper,the terahertz absorption spectra of gases mixed with 12 CO and 13 CO in the spec-trum range of 0.5–2.5 THz are measured by terahertz time-domain spectroscopy for the first time.Several isotopo-logues can be clearly distinguished based on the difference in their rotational energies and the consequent terahertz spectrum.The experimental results show that 12 CO and 13 CO have obvious characteristic absorption peaks in the spectrum range of 0.5–2.5 THz due to the difference in rotational energy,and the rotational constant B can be calculated according to the experimental values to distin-guish the two gaseous isotopologues.The frequency posi-tions of the characteristic absorption peak measured by this experiment and the rotation constant B calculated accord-ing to the experimental values are compared with those previous theoretical calculations and experimental results,and they are in good agreement.This result lays a foun-dation for developing more sophisticated terahertz instru-ments to the detection of different isotopologues.
基金supported by the National Key Research and Development Program of China(Grant No.2024YFF0808200)the National Natural Science Foundation of China(Grant Nos.42330104,42473035)+1 种基金Hubei Provincial Natural Science Foundation of China(Grant No.2025AFA005)the Most Special Fund from the State Key Laboratory of Geological Processes and Mineral Resources,China University of Geosciences(Grant Nos.MSFGPMR04 and MSFGPMR08)。
文摘LA-MC-ICP-MS offers the ability to directly measure small variations in isotope composition at the micrometer scale in geological samples.However,when analyzing isotope ratios in complex minerals,mass spectrometric interferences from themselves and isotopic mass fractionation arising from the analytical process need to be carefully corrected.The lack of professional software to visualize and process raw data presents a significant challenge for all of LA-MC-ICP-MS labs.Moreover,the rapid development of instruments and the innovations in micro-analytical techniques necessitate specialized software to complete the complex correction and improve efficiency,such as for in situ isochron dating or new linear regression correction techniques.In this study,Plume,a free data processing software,was developed specifically for isotopic data processing in LA-MC-ICP-MS.The software provides a comprehensive set of functions,including baseline correction,signal selection and integration,mass spectrometric interference correction,isotopic fractionation correction,uncertainty propagation,delta value calculation,and real-time data processing.It also supports a complete correction workflow for the latest isochron dating using LA-(MC)-ICP-MS/MS,including isotopic and elemental ratios correction,the isochron drawing,and age calculation for multiple models.Additionally,a dedicated linear regression calculation unit has been developed in Plume to specialize in data processing of low signal-to-noise ratio or high spatial resolution analysis.These new features expand the application range of LA-MC-ICP-MS and improve work efficiency through batch processing.
