Objective:From molecular,cell and systemic clinical treatment three levels,this report revealed the mechanism of wound healing,the basic theory and the importance of clinical practice for the Burn Regenerative Therapy...Objective:From molecular,cell and systemic clinical treatment three levels,this report revealed the mechanism of wound healing,the basic theory and the importance of clinical practice for the Burn Regenerative Therapy with MEBT/MEBO(BRT&MEBT/MEBO).Method:This report analyzed the importance and clinical curative effect for standardized appliance of BRT&MEBT/MEBO from two key points:1)Liquefaction of necrotic tissue from burn wound;2)Skin regeneration in situ.Result:There are three necessary conditions for skin physiologically repair and regeneration of burn wound:1)The formation of moist physiological environment on burn wound;2)The material foundation of life regenerative substances and histology for keratin-19 stem cells’regeneration in situ;3)Standardized procedures and appliance of BRT&MEBT/MEBO,which is the guarantee of burn wound healing physiologically.Conclusion:The theory of"Potential Regenerative Cell"and the technique of"Stem cell in situ regeneration"are the basic theory and clinical treatment for BRT.Full-thickness burn skin can be healed physiologically and the skin tissues and organs can be regenerated ONLY by standardized procedures and timely appliance of BRT&MEBT/MEBO.展开更多
Supported cell membrane coatings meet many requirements set to bioactive nanocarriers and materials,provided sidedness and fluidity of the natural membrane are maintained upon coating.However,the properties of a suppo...Supported cell membrane coatings meet many requirements set to bioactive nanocarriers and materials,provided sidedness and fluidity of the natural membrane are maintained upon coating.However,the properties of a support-surface responsible for maintaining correct sidedness and fluidity are unknown.Here,we briefly review the properties of natural membranes and membrane-isolation methods,with focus on the asymmetric distribution of functional groups in natural membranes(sidedness)and the ability of molecules to float across a membrane to form functional domains(fluidity).This review concludes that hydrophilic sugar-residues of glycoproteins in the outer-leaflet of cell membranes direct the more hydrophobic inner-leaflet towards a support-surface to create a correctly-sided membrane coating,regardless of electrostatic double-layer interactions.On positively-charged support-surfaces however,strong,electrostatic double-layer attraction of negatively-charged membranes can impede homogeneous coating.In correctly-sided membrane coatings,fluidity is maintained regardless of whether the surface carries a positive or negative charge.However,membranes are frozen on positively-charged,highly-curved,small nanoparticles and localized nanoscopic structures on a support-surface.This leaves an unsupported membrane coating in between nanostructures on planar support-surfaces that is in dual-sided contact with its aqueous environment,yielding enhanced fluidity in membrane coatings on nanostructured,planar support-surfaces as compared with smooth ones.展开更多
To the Editor:Autophagy is an evolutionarily conserved lysosome-mediated degradation process in eukaryotic cells that is crucial for maintaining cell homeostasis under normal conditions.Autophagy involves the formatio...To the Editor:Autophagy is an evolutionarily conserved lysosome-mediated degradation process in eukaryotic cells that is crucial for maintaining cell homeostasis under normal conditions.Autophagy involves the formation of a crescent-shaped phagophore(or the isolation membrane[IM]),which expands and closes to form a double-membraned autophagosome.The autophagosome is delivered to the late endosome/lysosomes for degradation of the sequestrated materials.Autophagic dysfunction can cause tumors and neurodegenerative,cardiovascular,and autoimmune diseases.展开更多
文摘Objective:From molecular,cell and systemic clinical treatment three levels,this report revealed the mechanism of wound healing,the basic theory and the importance of clinical practice for the Burn Regenerative Therapy with MEBT/MEBO(BRT&MEBT/MEBO).Method:This report analyzed the importance and clinical curative effect for standardized appliance of BRT&MEBT/MEBO from two key points:1)Liquefaction of necrotic tissue from burn wound;2)Skin regeneration in situ.Result:There are three necessary conditions for skin physiologically repair and regeneration of burn wound:1)The formation of moist physiological environment on burn wound;2)The material foundation of life regenerative substances and histology for keratin-19 stem cells’regeneration in situ;3)Standardized procedures and appliance of BRT&MEBT/MEBO,which is the guarantee of burn wound healing physiologically.Conclusion:The theory of"Potential Regenerative Cell"and the technique of"Stem cell in situ regeneration"are the basic theory and clinical treatment for BRT.Full-thickness burn skin can be healed physiologically and the skin tissues and organs can be regenerated ONLY by standardized procedures and timely appliance of BRT&MEBT/MEBO.
基金financially supported by the National Key Research and Development Program of China(2017YFE0131700)the National Natural Science Foundation of China(52293383)the Soochow University,the Nankai University,and UMCG,Groningen,The Netherlands.
文摘Supported cell membrane coatings meet many requirements set to bioactive nanocarriers and materials,provided sidedness and fluidity of the natural membrane are maintained upon coating.However,the properties of a support-surface responsible for maintaining correct sidedness and fluidity are unknown.Here,we briefly review the properties of natural membranes and membrane-isolation methods,with focus on the asymmetric distribution of functional groups in natural membranes(sidedness)and the ability of molecules to float across a membrane to form functional domains(fluidity).This review concludes that hydrophilic sugar-residues of glycoproteins in the outer-leaflet of cell membranes direct the more hydrophobic inner-leaflet towards a support-surface to create a correctly-sided membrane coating,regardless of electrostatic double-layer interactions.On positively-charged support-surfaces however,strong,electrostatic double-layer attraction of negatively-charged membranes can impede homogeneous coating.In correctly-sided membrane coatings,fluidity is maintained regardless of whether the surface carries a positive or negative charge.However,membranes are frozen on positively-charged,highly-curved,small nanoparticles and localized nanoscopic structures on a support-surface.This leaves an unsupported membrane coating in between nanostructures on planar support-surfaces that is in dual-sided contact with its aqueous environment,yielding enhanced fluidity in membrane coatings on nanostructured,planar support-surfaces as compared with smooth ones.
基金supported by grants from the National Natural Science Foundation of China(No.91954116)China Postdoctoral Science Foundation(No.2023M730128)the Key Support Project of Guo Zhong Health Care of China General Technology Group(No.GZKJ-KJXXQTHT-20230626).
文摘To the Editor:Autophagy is an evolutionarily conserved lysosome-mediated degradation process in eukaryotic cells that is crucial for maintaining cell homeostasis under normal conditions.Autophagy involves the formation of a crescent-shaped phagophore(or the isolation membrane[IM]),which expands and closes to form a double-membraned autophagosome.The autophagosome is delivered to the late endosome/lysosomes for degradation of the sequestrated materials.Autophagic dysfunction can cause tumors and neurodegenerative,cardiovascular,and autoimmune diseases.
