Standard bacterial suspensions play a crucial role in microbiological diagnosis.Traditional prepar-ation methods,which rely heavily on manual operations,face challenges such as poor reproducibility,low ef-ficiency,and...Standard bacterial suspensions play a crucial role in microbiological diagnosis.Traditional prepar-ation methods,which rely heavily on manual operations,face challenges such as poor reproducibility,low ef-ficiency,and biosafety concerns.In this study,we propose a high-precision automated colony extraction and separation system that combines large-field imaging and artificial intelligence(AI)to facilitate intelligent screening and localization of colonies.Firstly,a large-field imaging system was developed to capture high-resolution images of 90 mm Petri dishes,achieving a physical resolution of 13.2μm and an imaging speed of 13 frames per second.Subsequently,AI technology was employed for the automatic recognition and localiza-tion of colonies,enabling the selection of target colonies with diameters ranging from 1.9 to 2.3 mm.Next,a three-axis motion control platform was designed,accompanied by a path planning algorithm for the efficient extraction of colonies.An electronic pipette was employed for accurate colony collection.Additionally,a bacterial suspension concentration measurement module was developed,incorporating a 650 nm laser diode as the light source,achieving a measurement accuracy of 0.01 McFarland concentration(MCF).Finally,the system’s performance was validated through the preparation of an Esckerichia coli(E.coli)suspension.After 17 hours of cultivation,E.coli was extracted four times,achieving the target concentration set by the system.This work is expected to enable rapid and accurate microbial sample preparation,significantly reducing de-tection cycles and alleviating the workload of healthcare personnel.展开更多
Objective This study aims to investigate the joint associations of sarcopenia and social isolation with mortality risk.Methods Using data from the Chinese Longitudinal Healthy Longevity Survey(CLHLS)and the UK Biobank...Objective This study aims to investigate the joint associations of sarcopenia and social isolation with mortality risk.Methods Using data from the Chinese Longitudinal Healthy Longevity Survey(CLHLS)and the UK Biobank,sarcopenia was diagnosed according to European and Asian Working Groups for Sarcopenia criteria.Social isolation was assessed using standardized questionnaires,including questions on solitude,frequency of social activities,contact with others,and marital status(for the CLHLS only).Results During the follow-up period,8,249 deaths occurred in the CLHLS and 26,670 deaths in the UK Biobank groups.While no significant interaction was observed between sarcopenia and social isolation in predicting all-cause mortality in the CLHLS cohort,the association between social isolation and mortality was stronger among individuals with sarcopenia in the UK Biobank(P-interaction=0.03,relative risk due to interaction:0.23,95%confidence interval[CI]:0.06–0.41).Further joint analyses showed that participants with sarcopenia and high levels of social isolation had the highest mortality risk(hazard ration[HR]:1.99;95%CI:[1.74–2.28]in the CLHLS and 1.69[1.55–1.85]in the UK Biobank)compared to those without either condition.Conclusion The combination of social isolation and sarcopenia synergistically increases the risk of mortality in middle-aged and older adults across diverse populations.展开更多
Isolation technology can reduce the type of structural damage that earthquakes cause.A new type of composite sliding-rolling friction composite seismic isolation bearing(SRF)with composite sliding friction and rolling...Isolation technology can reduce the type of structural damage that earthquakes cause.A new type of composite sliding-rolling friction composite seismic isolation bearing(SRF)with composite sliding friction and rolling friction is proposed.SRF is capable of realizing a parallel arrangement of sliding friction and rolling friction,and the coefficient of dynamic friction shows variability.The proposed static tests on composite bearings were conducted to investigate the effects of the number of shims,loading speed and vertical pressure on the dynamic friction factor.Test results show that the coefficient of dynamic friction first generally decreases and then increases with an increase in sliding speed,prior to again decreasing with an increase in vertical pressure.The dynamic friction factor increases and then decreases with an increase in the number of shims for a four-roll ball.It decreases and then increases with an increase in the number of shims for a five-roll ball.Based on finite element analysis,modeling and analyzing the effects of the coefficient of friction,the number of balls and the number of shims on the hysteresis performance of the support and derive its skeleton curve.The SRF hysteretic performance,dynamic friction factor and the number of rolling balls and shims show significant correlation.展开更多
Natural hybridization is known to play a vital role in speciation;however,the mechanisms underlying the early stages of natural hybridization remain unclear.Where two plant species come into contact,two driving forces...Natural hybridization is known to play a vital role in speciation;however,the mechanisms underlying the early stages of natural hybridization remain unclear.Where two plant species come into contact,two driving forces may balance the dynamic consequences of hybridization:fusion by hybridization-mediated gene flow,and separation by reproductive isolation(RI)(Ma et al.,2010a,b;Chang et al.,2022).展开更多
An attack-resilient distributed Nash equilibrium(NE) seeking problem is addressed for noncooperative games of networked systems under malicious cyber-attacks,i.e.,false data injection(FDI) attacks.Different from many ...An attack-resilient distributed Nash equilibrium(NE) seeking problem is addressed for noncooperative games of networked systems under malicious cyber-attacks,i.e.,false data injection(FDI) attacks.Different from many existing distributed NE seeking works,it is practical and challenging to get resilient adaptively distributed NE seeking under unknown and unbounded FDI attacks.An attack-resilient NE seeking algorithm that is distributed(i.e.,independent of global information on the graph's algebraic connectivity,Lipschitz and monotone constants of pseudo-gradients,or number of players),is presented by means of incorporating the consensus-based gradient play with a distributed attack identifier so as to achieve simultaneous NE seeking and attack identification asymptotically.Another key characteristic is that FDI attacks are allowed to be unknown and unbounded.By exploiting nonsmooth analysis and stability theory,the global asymptotic convergence of the developed algorithm to the NE is ensured.Moreover,we extend this design to further consider the attack-resilient NE seeking of double-integrator players.Lastly,numerical simulation and practical experiment results are presented to validate the developed algorithms' effectiveness.展开更多
在全球产业链重构和双循环新发展格局背景下,双向FDI(Foreign Direct Investment)如何助力我国产业结构升级成为关注焦点。本文基于2010—2023年省际面板数据,构建回归模型和门槛模型,实证检验了双向FDI协调发展对产业结构合理化与高度...在全球产业链重构和双循环新发展格局背景下,双向FDI(Foreign Direct Investment)如何助力我国产业结构升级成为关注焦点。本文基于2010—2023年省际面板数据,构建回归模型和门槛模型,实证检验了双向FDI协调发展对产业结构合理化与高度化的异质动态影响。结果显示,双向FDI协调发展显著推动了我国产业结构升级,对产业结构合理化具有稳定的正向影响,对产业结构高度化存在双重门槛效应,呈现“低效—提效—高效”的演化特征。展开更多
文摘Standard bacterial suspensions play a crucial role in microbiological diagnosis.Traditional prepar-ation methods,which rely heavily on manual operations,face challenges such as poor reproducibility,low ef-ficiency,and biosafety concerns.In this study,we propose a high-precision automated colony extraction and separation system that combines large-field imaging and artificial intelligence(AI)to facilitate intelligent screening and localization of colonies.Firstly,a large-field imaging system was developed to capture high-resolution images of 90 mm Petri dishes,achieving a physical resolution of 13.2μm and an imaging speed of 13 frames per second.Subsequently,AI technology was employed for the automatic recognition and localiza-tion of colonies,enabling the selection of target colonies with diameters ranging from 1.9 to 2.3 mm.Next,a three-axis motion control platform was designed,accompanied by a path planning algorithm for the efficient extraction of colonies.An electronic pipette was employed for accurate colony collection.Additionally,a bacterial suspension concentration measurement module was developed,incorporating a 650 nm laser diode as the light source,achieving a measurement accuracy of 0.01 McFarland concentration(MCF).Finally,the system’s performance was validated through the preparation of an Esckerichia coli(E.coli)suspension.After 17 hours of cultivation,E.coli was extracted four times,achieving the target concentration set by the system.This work is expected to enable rapid and accurate microbial sample preparation,significantly reducing de-tection cycles and alleviating the workload of healthcare personnel.
基金supported by grants from the National Key Research and Development Program of China(No.2023YFC3606300,No.2022YFC3600300)the National Natural Science Foundation of China(No.82325043)the National Key Research and Development Program of Hubei Province(2022BCA036)。
文摘Objective This study aims to investigate the joint associations of sarcopenia and social isolation with mortality risk.Methods Using data from the Chinese Longitudinal Healthy Longevity Survey(CLHLS)and the UK Biobank,sarcopenia was diagnosed according to European and Asian Working Groups for Sarcopenia criteria.Social isolation was assessed using standardized questionnaires,including questions on solitude,frequency of social activities,contact with others,and marital status(for the CLHLS only).Results During the follow-up period,8,249 deaths occurred in the CLHLS and 26,670 deaths in the UK Biobank groups.While no significant interaction was observed between sarcopenia and social isolation in predicting all-cause mortality in the CLHLS cohort,the association between social isolation and mortality was stronger among individuals with sarcopenia in the UK Biobank(P-interaction=0.03,relative risk due to interaction:0.23,95%confidence interval[CI]:0.06–0.41).Further joint analyses showed that participants with sarcopenia and high levels of social isolation had the highest mortality risk(hazard ration[HR]:1.99;95%CI:[1.74–2.28]in the CLHLS and 1.69[1.55–1.85]in the UK Biobank)compared to those without either condition.Conclusion The combination of social isolation and sarcopenia synergistically increases the risk of mortality in middle-aged and older adults across diverse populations.
文摘Isolation technology can reduce the type of structural damage that earthquakes cause.A new type of composite sliding-rolling friction composite seismic isolation bearing(SRF)with composite sliding friction and rolling friction is proposed.SRF is capable of realizing a parallel arrangement of sliding friction and rolling friction,and the coefficient of dynamic friction shows variability.The proposed static tests on composite bearings were conducted to investigate the effects of the number of shims,loading speed and vertical pressure on the dynamic friction factor.Test results show that the coefficient of dynamic friction first generally decreases and then increases with an increase in sliding speed,prior to again decreasing with an increase in vertical pressure.The dynamic friction factor increases and then decreases with an increase in the number of shims for a four-roll ball.It decreases and then increases with an increase in the number of shims for a five-roll ball.Based on finite element analysis,modeling and analyzing the effects of the coefficient of friction,the number of balls and the number of shims on the hysteresis performance of the support and derive its skeleton curve.The SRF hysteretic performance,dynamic friction factor and the number of rolling balls and shims show significant correlation.
基金supported by the National Natural Science Foundation of China(U23A20160,32360336)Guizhou Provincial Key Technology R&D Program(Qian KeHe ZhiCheng[2023]YiBan035).
文摘Natural hybridization is known to play a vital role in speciation;however,the mechanisms underlying the early stages of natural hybridization remain unclear.Where two plant species come into contact,two driving forces may balance the dynamic consequences of hybridization:fusion by hybridization-mediated gene flow,and separation by reproductive isolation(RI)(Ma et al.,2010a,b;Chang et al.,2022).
基金supported in part by the National Natural Science Foundation of China(62373022,U2241217,62141604)Beijing Natural Science Foundation(4252043,JQ23019)+4 种基金the Fundamental Research Funds for the Central Universities(JKF-2025037448805,JKF-2025086098295)the Aeronautical Science Fund(2023Z034051001)the Academic Excellence Foundation of BUAA for Ph.D. Studentsthe Science and Technology Innovation2030—Key Project of New Generation Artificial Intelligence(2020AAA0108200)the National Key Research and Development Program of China(2022YFB3305600)。
文摘An attack-resilient distributed Nash equilibrium(NE) seeking problem is addressed for noncooperative games of networked systems under malicious cyber-attacks,i.e.,false data injection(FDI) attacks.Different from many existing distributed NE seeking works,it is practical and challenging to get resilient adaptively distributed NE seeking under unknown and unbounded FDI attacks.An attack-resilient NE seeking algorithm that is distributed(i.e.,independent of global information on the graph's algebraic connectivity,Lipschitz and monotone constants of pseudo-gradients,or number of players),is presented by means of incorporating the consensus-based gradient play with a distributed attack identifier so as to achieve simultaneous NE seeking and attack identification asymptotically.Another key characteristic is that FDI attacks are allowed to be unknown and unbounded.By exploiting nonsmooth analysis and stability theory,the global asymptotic convergence of the developed algorithm to the NE is ensured.Moreover,we extend this design to further consider the attack-resilient NE seeking of double-integrator players.Lastly,numerical simulation and practical experiment results are presented to validate the developed algorithms' effectiveness.
文摘在全球产业链重构和双循环新发展格局背景下,双向FDI(Foreign Direct Investment)如何助力我国产业结构升级成为关注焦点。本文基于2010—2023年省际面板数据,构建回归模型和门槛模型,实证检验了双向FDI协调发展对产业结构合理化与高度化的异质动态影响。结果显示,双向FDI协调发展显著推动了我国产业结构升级,对产业结构合理化具有稳定的正向影响,对产业结构高度化存在双重门槛效应,呈现“低效—提效—高效”的演化特征。
