Large flowers are typically more conspicuous to pollinators and are associated with more nutritional rewards than small flowers.Flower size can also determine flower temperature,which can be a reward for flower-visiti...Large flowers are typically more conspicuous to pollinators and are associated with more nutritional rewards than small flowers.Flower size can also determine flower temperature,which can be a reward for flower-visiting insects.Nectarless Royal irises offer overnight shelter and morning warmth to male Eucera bee pollinators.A dark spot on their lower tepals (black patch) may act as a visual cue and contribute to flower heating.Here,we examined the association of floral display (i.e.flower size and black patch size) with flower temperature and female fitness (i.e.seed set) in Royal irises populations across an aridity gradient.First,we tested pollinator preference using artificial flowers of varying sizes.Next,we assessed associations between flower and black patch size,flower warming rate and female fitness.Finally,we manipulated flowers in the field to determine if the black patch influences heating.Pollinators preferred larger artificial flowers for overnight shelter.However,selection for larger flowers was found only in the population with the smallest flowers.No association was found between flower/black patch size and floral heating in natural populations,and the black patch did not affect flower heating.Flowers reached the temperature threshold for bees to start flying (18 ℃) 10-35 min faster than ambient air.We conclude that the large flower size in the Royal irises serves as a visual signal,advertising for night shelter and flowers—independently of the size—heat up faster in the morning than ambient air.Flowers thus,potentially offer a ‘head start’ to the flower-dwelling bees,allowing them to warm up more quickly than in outside conditions.展开更多
Iris domestica,a perennial herb of the Iridaceae family,is widely recognized for its rich isoflavone content and broad therapeutic properties.To elucidate the biosynthetic pathway of these medicinally significant comp...Iris domestica,a perennial herb of the Iridaceae family,is widely recognized for its rich isoflavone content and broad therapeutic properties.To elucidate the biosynthetic pathway of these medicinally significant compounds,we constructed a haplotype-resolved genome assembly of this species.Transcriptomic and metabolomic analyses revealed tissue-specific accumulation of isoflavone,particularly in rhizomes and roots.Functional characterization identified two candidate isoflavone synthase genes,among which IdIFS was confirmed to promote the biosynthesis of key compounds tectorigenin and irisflorentin.The high-quality genome assembly presented here provides a foundational resource for further research into the evolution,secondary metabolite,and environmental adaptation of I.domestica.展开更多
The sustainable sourcing of novel bioactive compounds from natural sources is crucial to the success of the pharmaceutical,food,and cosmetics industries.Iris bucharica Foster(syn.Juno bucharica(Foster)Vved.)is a promi...The sustainable sourcing of novel bioactive compounds from natural sources is crucial to the success of the pharmaceutical,food,and cosmetics industries.Iris bucharica Foster(syn.Juno bucharica(Foster)Vved.)is a promising source of novel bioactive molecules,particularly phenolic compounds,which are renowned for their antioxidant properties.In this study,we developed a reliable HPLC-UV-DAD method to identify and quantify phenolic compounds in the leaves and bulbs of I.bucharica,establishing the first set of quality control markers for this species.A total of 21 phenolic compounds were identified in the leaves,with flavonoids isoorientin,guaijaverin,hyperoside,and cosmosiin,the isoflavonoid biochanin A,and the simple phenolic ferulic acid being the most prominent.In comparison,14 compounds were identified in the bulbs,primarily isoflavonoids,including tectoridin and germanaism B,and flavonoid apigenin.The leaves extracts exhibited significant antioxidant activity,whereas the anti-allergic and anti-inflammatory effects were mild.These findings highlight I.bucharica as a sustainable source of bioactive compounds with potential industrial applications.However,further studies are needed to evaluate bioavailability and in vivo efficacy,as well as to optimise extraction methods to realize its industrial potential fully.展开更多
AIM:To preliminary explore the safety and effectiveness of artificial iris implantation.METHODS:Fourteen patients with iris defects who underwent artificial iris implantation at Hainan Boao Super Hospital from June 20...AIM:To preliminary explore the safety and effectiveness of artificial iris implantation.METHODS:Fourteen patients with iris defects who underwent artificial iris implantation at Hainan Boao Super Hospital from June 2020 to September 2021 were retrospectively analyzed for safety and effectiveness of the surgery by comparing the preoperative and postoperative best-corrected visual acuity(BCVA),intraocular pressure(IOP),corneal endothelial cell density(ECD),ocular axial length(AL),anterior chamber depth(ACD),patient satisfaction of photophobia and appearance improvement,and postoperative complications.RESULTS:The mean age was 37.21±14.85(7-60)y,including 13 males and 1 female.The mean follow-up period was 4.64±2.32(1-10)mo.The mean AL was 24.00±1.06(21.68-25.58)mm.The postoperative mean anterior chamber depth measured was 4.07±0.75(2.61-5.07)mm.The mean BCVA was 0.69±0.65 logMAR preoperatively and 0.46±0.60 logMAR at the last follow-up time(P=0.36).There was no significant differences in the IOP preoperative and postoperative(14.14±3.10 and 13.65±3.08 mm Hg,respectively,P=0.69).The preoperative ECD was 1674.09±566.11 per 1 mm^(2),and the postoperative ECD was 1439.45±425.15 per 1 mm^(2)(P=0.21).No obvious corneal opacity or corneal decompensation was observed in all patients.The preoperative and postoperative photophobia scores were 8.50±1.55 and 4.50±1.94,respectively(P<0.05),and the preoperative and postoperative appearance defect scores were 6.58±2.98 and 2.75±1.69,respectively(P<0.05).Among the 14 patients,one had artificial iris displacement,two had a transient IOP rise after treatment of antiglaucomatous eyedrops.No complications were observed during surgery.CONCLUSION:The novel artificial iris material is safe,which can significantly improve the appearance defects and photophobia,improve the postoperative visual acuity to a certain extent.This surgery had a high patient satisfaction rate with few and controllable postoperative complications.At the same time,the artificial iris,with its diverse configurations,variable colors,arbitrary cutting,and various surgical procedures,fully realizes personalized treatment,which solves the clinical problem of iris defect.展开更多
This study aimed to differentiate between the therapeutic and purely cosmetic aspects of keratopigmentation by examining the results of previous studies.Corneal opacity,corneal leukoma,and iris abnormalities represent...This study aimed to differentiate between the therapeutic and purely cosmetic aspects of keratopigmentation by examining the results of previous studies.Corneal opacity,corneal leukoma,and iris abnormalities represent the primary reasons for keratopigmentation in therapeutic settings.In therapeutic contexts,the most commonly reported adverse effects included inflammation,foreign body sensation,light sensitivity,reduced intraocular pressure,and neovascularization.The side effects most frequently noted in purely cosmetic applications were photophobia and ectasia.In therapeutic scenarios,patients who experience pre-operative vision impairment often report not only the absence of visual loss post-procedure but also an enhancement in visual acuity,accompanied by alleviation of symptoms such as glare.In purely cosmetic procedures,visual acuity tends to remain stable.Research indicates that dissatisfaction with keratopigmentation results is more prevalent in studies aimed at therapeutic interventions compared to cosmetic enhancements.Furthermore,instances of discoloration and fading are more frequently observed in therapeutic cases than in cosmetic ones,including the need for retouching.The use of keratopigmentation in both therapeutic and cosmetic settings produces positive and acceptable results,especially in cosmetic applications.Nevertheless,complications are more commonly observed in therapeutic situations,which underscores the importance of candidate selection for keratopigmentation procedures.展开更多
The mutualistic symbiotic system formed by clumping arbuscular mycorrhizal fungi(AMF)and plants can remediate heavy metal-contaminated soils.However,the specific mechanisms underlying the interaction between AMF and i...The mutualistic symbiotic system formed by clumping arbuscular mycorrhizal fungi(AMF)and plants can remediate heavy metal-contaminated soils.However,the specific mechanisms underlying the interaction between AMF and inter-root microbial communities,particularly their impact on organic phosphorus(P)cycling,remain unclear.This study investigated the gene regulation processes involved in inter-root soil phosphorus cycling in wetland plants,specifically Iris tectorum,following inoculation with AMF under varying concentrations of chromium(Cr)stress.Through macro-genome sequencing,we analyzed the composition and structure of the inter-root soil microbial community associated with Iris tectorum under greenhouse pot conditions.The results demonstrated significant changes in the diversity and composition of the inter-root soil microbial community following AMF inoculation,with Proteobacteria,Actinobacteria,Chloroflexi,Acidobacteria,and Bacteroidetes being the dominant taxa.Under Cr stress,species and gene co-occurrence network analysis revealed that AMF promoted the transformation process of organic phosphorus mineralization and facilitated inorganic phosphorus uptake.Additionally,network analysis of functional genes indicated strong aggregation of(pstS,pstA,pstC,TC.PIT,phoR,pp-gppA)genes,which collectively enhanced phosphorus uptake by plants.These findings shed light on the inter-root soil phosphorus cycling process during the co-remediation of Cr-contaminated soil by AMF-Iris tectorum symbiosis,providing valuable theoretical support for the application of AMF-wetland plant symbiosis systems to remediate heavy metal-contaminated soil.展开更多
AIM:To compare the efficacy of different administration regimens of compound tropicamide eyedrops(CTE)for pupil dilation for children with dark iris.METHODS:A prospective,comparative,randomized interventional study wa...AIM:To compare the efficacy of different administration regimens of compound tropicamide eyedrops(CTE)for pupil dilation for children with dark iris.METHODS:A prospective,comparative,randomized interventional study was conducted.Children in Group 1 received CTE 3 times with a 3min interval between each application.Children in Group 2 received CTE 4 times with a 5min interval between each application.We measured their pupil diameters at baseline(pre-drug instillation)and 30min and 60min post-drug instillation and assessed the pupillary light reflex at 60min post-drug instillation.RESULTS:In total,194 eyes of 101 children were enrolled.The changes of pupil diameter at 30min and 60min post-drug instillation were 1.2±0.6 mm and 2.3±1.0 mm in Group 1,and 2.3±0.9 mm and 3.7±1.0 mm in Group 2,respectively.Group 2 showed a larger change in pupil size than Group 1 at 30min(P<0.01)and 60min(P<0.01).The effect of pupil dilation in Group 2 was 1.25 times that in Group 1.The change in pupil size was positively associated with age.A higher proportion of children in Group 1 had smaller pupil diameter and reactive pupils at the final time point,with only 33 children(33.7%)had final pupil size≥6.5 mm,and only 9 children(9.2%)had non-reactive pupils.Children in Group 2 achieved larger pupil diameter and more nonreactive pupils at the final time point,with 84 children(87.5%)had final pupil size≥6.5 mm,and only 22 children(22.9%)had reactive pupils.CONCLUSION:Increasing the frequency of compound tropicamide and lengthening the interval between eye drop applications can produce stronger mydriatic effects.展开更多
In northern China,light and temperature are major limiting factors for plant growth,particularly during seed production and seedling establishment.While previous studies suggested a possible role for the MYB97 gene in...In northern China,light and temperature are major limiting factors for plant growth,particularly during seed production and seedling establishment.While previous studies suggested a possible role for the MYB97 gene in cold-stress,confirmation through documented evidence was lacking.In this study,we transformed the MYB97 gene from Iris laevigata into tobacco,and discovered that the gene boosted photosynthesis,photoprotection and resilience to cold.The transgenic tobacco seeds exhibited enhanced germination and accelerated seedling growth.Moreover,these plants had decreased levels of MDA(Malondialdehyde)and relative conductance,coupled with elevated concentrations of proline and soluble sugars.This response was accompanied by heightened activity of antioxidant enzymes during periods of cold stress(4 and−2℃).Exposure to low temperatures(0–15℃)also reduced heights but accentuated primary root growth in transgenic tobacco plants.Additionally,tobacco leaves showed an increased growth along with higher chlorophyll levels,net photosynthetic rates,stomatal conductance,transpiration rates and non-photochemical quenching coefficient.This study shows that IlMYB97(The MYB97 genes in I.laevigata)improves cold-resistance,and enhances photosynthesis and photoprotective ability,and thus overall growth and development.These findings would offer the genetic resources to further study cold resistance and photosynthesis.展开更多
Iris sanguinea Donn ex Horn.is a common perennial ornamental herb in Northeast China due to its strong cold-resistance capacity.In this study,integrative analyses of metabolomics and transcriptomics data were performe...Iris sanguinea Donn ex Horn.is a common perennial ornamental herb in Northeast China due to its strong cold-resistance capacity.In this study,integrative analyses of metabolomics and transcriptomics data were performed on five cultivars of I.sanguinea with varying petal colors to elucidate the molecular basis underlying petal pigmentation.The metabolomics data revealed that a total of five major anthocyanins were responsible for petal pigmentation in I.sanguinea.While two delphinidin-3-O-glucoside derivatives contributed to blue coloration in petals,two petunidin-3-O-glucoside and cyanidin-3-O-rutinoside derivatives exhibited significantly higher abundances in the deep pink petals.In addition,transcriptomics revealed a gradual increase in the expression of a large set of genes involved in flavonoid and anthocyanin biosynthesis pathways,corresponding to the varying levels of anthocyanin content among cultivar petals.Integrated metabolomics and transcriptomics analysis further led to the discovery of molecular modules controlling petal pigmentation,in which an R2R3 MYB transcription factor IsMYBL1 was found to be associated with the accumulation of key anthocyanin derivatives,it localized to the nucleus.The regulatory role of IsMYBL1 in anthocyanin accumulation has been validated through overexpressing in tobacco and knockdown in I.sanguinea using an improved virusinduced gene silencing(VIGS)approach.Finally,BiFC and Y2H showed that IsMYBL1 interacts with IsEGL3 and IsTTG1 to activate the expression of genes involved in anthocyanin biosynthesis,Yeast one-hybrid,dual-luciferase assays,showed that IsMYBL1 significantly increased anthocyanin contents in I.sanguinea by promoting the expression of IsANS,thereby promoting anthocyanin accumulation.Taken together,our study has provided a comprehensive molecular profiling of petal coloration in I.sanguinea and revealed key gene/metabolite networks that determine petal pigmentation.展开更多
Phakic intraocular lens implantation has become one of the important means of correcting refractive errors today.Among them,the implantable collamer lens(ICL)is favored for its wide range of correction,excellent optic...Phakic intraocular lens implantation has become one of the important means of correcting refractive errors today.Among them,the implantable collamer lens(ICL)is favored for its wide range of correction,excellent optical quality,and high safety,but the risks of postoperative complications such as glaucoma and anterior subcapsular opacification still exist.Vault is an important indicator for evaluating the safety after ICL implantation,and its ideal state is crucial for preventing complications.Studies have shown that iris morphology has a significant impact on vault.In order to further optimize surgical outcomes and improve surgical safety,this review comprehensively reviews the research progress of iris-related parameters in ICL implantation and discusses the importance of various parameters in preoperative evaluation and postoperative follow-up.展开更多
In vivo imaging of human iris vasculature remains a persistent challenge,limiting our understanding of its relationship with ocular disease pathogenesis.Conventional raster scan optical coherence tomography angiograph...In vivo imaging of human iris vasculature remains a persistent challenge,limiting our understanding of its relationship with ocular disease pathogenesis.Conventional raster scan optical coherence tomography angiography(OCTA)suffers from angular-dependent contrast(including blind spots),limited field of view,and prolonged imaging time—challenges that restrict its clinical utility.We introduce a circular interleaving scan OCTA method that overcomes these barriers by enabling 360 deg high-contrast iris angiography with consistent spatiotemporal sampling and optimized motion contrast.The circular scan design enables directionoptimized sampling:we configured circumferential sampling density to approximately twice the radial density,enhancing detection of radially oriented iris vasculature.A Cartesian–polar coordinate transformation was implemented for eye-motion compensation,vessel realignment,and vasculature reconstruction.Compared with raster scan OCTA,our circular scan protocol demonstrates 1.55×higher efficiency in iris vascular imaging,featuring a superior duty cycle(99.95%versus 82.00%)and eliminating redundant data acquisition from rectangular field corners(27.3%of the circular area).This method improves vessel density measurement by 39.0%and vessel count quantification by 25.2%relative to raster scans.By eliminating angular-dependent blind spots,our method significantly enhances vascular quantification reliability,paving the way to a better understanding of ocular diseases and holding promising potential for future clinical applications.展开更多
基金supported by a postdoctoral fellowship from the University of Potsdam-Tel Aviv University collaboration program to SLGby funding from the Israel Science Foundation (336/16) to Y.S.+1 种基金from the Human Frontier Science Program (RGP023/2023, https://doi.org/10.52044/HFSP.RGP0232023.pc.gr.168611)AFOSR (FA8655-23-1-7049) to C.J.v.d.K.
文摘Large flowers are typically more conspicuous to pollinators and are associated with more nutritional rewards than small flowers.Flower size can also determine flower temperature,which can be a reward for flower-visiting insects.Nectarless Royal irises offer overnight shelter and morning warmth to male Eucera bee pollinators.A dark spot on their lower tepals (black patch) may act as a visual cue and contribute to flower heating.Here,we examined the association of floral display (i.e.flower size and black patch size) with flower temperature and female fitness (i.e.seed set) in Royal irises populations across an aridity gradient.First,we tested pollinator preference using artificial flowers of varying sizes.Next,we assessed associations between flower and black patch size,flower warming rate and female fitness.Finally,we manipulated flowers in the field to determine if the black patch influences heating.Pollinators preferred larger artificial flowers for overnight shelter.However,selection for larger flowers was found only in the population with the smallest flowers.No association was found between flower/black patch size and floral heating in natural populations,and the black patch did not affect flower heating.Flowers reached the temperature threshold for bees to start flying (18 ℃) 10-35 min faster than ambient air.We conclude that the large flower size in the Royal irises serves as a visual signal,advertising for night shelter and flowers—independently of the size—heat up faster in the morning than ambient air.Flowers thus,potentially offer a ‘head start’ to the flower-dwelling bees,allowing them to warm up more quickly than in outside conditions.
文摘Iris domestica,a perennial herb of the Iridaceae family,is widely recognized for its rich isoflavone content and broad therapeutic properties.To elucidate the biosynthetic pathway of these medicinally significant compounds,we constructed a haplotype-resolved genome assembly of this species.Transcriptomic and metabolomic analyses revealed tissue-specific accumulation of isoflavone,particularly in rhizomes and roots.Functional characterization identified two candidate isoflavone synthase genes,among which IdIFS was confirmed to promote the biosynthesis of key compounds tectorigenin and irisflorentin.The high-quality genome assembly presented here provides a foundational resource for further research into the evolution,secondary metabolite,and environmental adaptation of I.domestica.
基金supported by grants from the National Science and Technology Council(NSTC),Taiwan(NSTC 114-2320-B-037-020-MY3,113-2320-B-037-023,and 112-2320-B-037-012 granted to M.K.,113-2321-B-255-001,113-2321-B-182-003,112-2321-B-182-003,112-2321-B-255-001,111-2320-B-255-006-MY3,and 111-2321-B-255-001 granted to T.L.H.)Kaohsiung Medical University Research Foundation[KMU-Q113011 awarded to M.K.and KMU-M114020 awarded to B.H.C.]NSYSU-KMU joint research project(NSYSU-KMU-114-P16)awarded to M.K.
文摘The sustainable sourcing of novel bioactive compounds from natural sources is crucial to the success of the pharmaceutical,food,and cosmetics industries.Iris bucharica Foster(syn.Juno bucharica(Foster)Vved.)is a promising source of novel bioactive molecules,particularly phenolic compounds,which are renowned for their antioxidant properties.In this study,we developed a reliable HPLC-UV-DAD method to identify and quantify phenolic compounds in the leaves and bulbs of I.bucharica,establishing the first set of quality control markers for this species.A total of 21 phenolic compounds were identified in the leaves,with flavonoids isoorientin,guaijaverin,hyperoside,and cosmosiin,the isoflavonoid biochanin A,and the simple phenolic ferulic acid being the most prominent.In comparison,14 compounds were identified in the bulbs,primarily isoflavonoids,including tectoridin and germanaism B,and flavonoid apigenin.The leaves extracts exhibited significant antioxidant activity,whereas the anti-allergic and anti-inflammatory effects were mild.These findings highlight I.bucharica as a sustainable source of bioactive compounds with potential industrial applications.However,further studies are needed to evaluate bioavailability and in vivo efficacy,as well as to optimise extraction methods to realize its industrial potential fully.
文摘AIM:To preliminary explore the safety and effectiveness of artificial iris implantation.METHODS:Fourteen patients with iris defects who underwent artificial iris implantation at Hainan Boao Super Hospital from June 2020 to September 2021 were retrospectively analyzed for safety and effectiveness of the surgery by comparing the preoperative and postoperative best-corrected visual acuity(BCVA),intraocular pressure(IOP),corneal endothelial cell density(ECD),ocular axial length(AL),anterior chamber depth(ACD),patient satisfaction of photophobia and appearance improvement,and postoperative complications.RESULTS:The mean age was 37.21±14.85(7-60)y,including 13 males and 1 female.The mean follow-up period was 4.64±2.32(1-10)mo.The mean AL was 24.00±1.06(21.68-25.58)mm.The postoperative mean anterior chamber depth measured was 4.07±0.75(2.61-5.07)mm.The mean BCVA was 0.69±0.65 logMAR preoperatively and 0.46±0.60 logMAR at the last follow-up time(P=0.36).There was no significant differences in the IOP preoperative and postoperative(14.14±3.10 and 13.65±3.08 mm Hg,respectively,P=0.69).The preoperative ECD was 1674.09±566.11 per 1 mm^(2),and the postoperative ECD was 1439.45±425.15 per 1 mm^(2)(P=0.21).No obvious corneal opacity or corneal decompensation was observed in all patients.The preoperative and postoperative photophobia scores were 8.50±1.55 and 4.50±1.94,respectively(P<0.05),and the preoperative and postoperative appearance defect scores were 6.58±2.98 and 2.75±1.69,respectively(P<0.05).Among the 14 patients,one had artificial iris displacement,two had a transient IOP rise after treatment of antiglaucomatous eyedrops.No complications were observed during surgery.CONCLUSION:The novel artificial iris material is safe,which can significantly improve the appearance defects and photophobia,improve the postoperative visual acuity to a certain extent.This surgery had a high patient satisfaction rate with few and controllable postoperative complications.At the same time,the artificial iris,with its diverse configurations,variable colors,arbitrary cutting,and various surgical procedures,fully realizes personalized treatment,which solves the clinical problem of iris defect.
基金Supported by Noor Ophthalmology Research Center.
文摘This study aimed to differentiate between the therapeutic and purely cosmetic aspects of keratopigmentation by examining the results of previous studies.Corneal opacity,corneal leukoma,and iris abnormalities represent the primary reasons for keratopigmentation in therapeutic settings.In therapeutic contexts,the most commonly reported adverse effects included inflammation,foreign body sensation,light sensitivity,reduced intraocular pressure,and neovascularization.The side effects most frequently noted in purely cosmetic applications were photophobia and ectasia.In therapeutic scenarios,patients who experience pre-operative vision impairment often report not only the absence of visual loss post-procedure but also an enhancement in visual acuity,accompanied by alleviation of symptoms such as glare.In purely cosmetic procedures,visual acuity tends to remain stable.Research indicates that dissatisfaction with keratopigmentation results is more prevalent in studies aimed at therapeutic interventions compared to cosmetic enhancements.Furthermore,instances of discoloration and fading are more frequently observed in therapeutic cases than in cosmetic ones,including the need for retouching.The use of keratopigmentation in both therapeutic and cosmetic settings produces positive and acceptable results,especially in cosmetic applications.Nevertheless,complications are more commonly observed in therapeutic situations,which underscores the importance of candidate selection for keratopigmentation procedures.
基金supported by 2024 Guizhou Basic Research Plan(Natural Science)Project,China(Foundation of Guizhou science cooperation-ZK[2024]General 490)the National Natural Science Foundation of China(No.31560107).
文摘The mutualistic symbiotic system formed by clumping arbuscular mycorrhizal fungi(AMF)and plants can remediate heavy metal-contaminated soils.However,the specific mechanisms underlying the interaction between AMF and inter-root microbial communities,particularly their impact on organic phosphorus(P)cycling,remain unclear.This study investigated the gene regulation processes involved in inter-root soil phosphorus cycling in wetland plants,specifically Iris tectorum,following inoculation with AMF under varying concentrations of chromium(Cr)stress.Through macro-genome sequencing,we analyzed the composition and structure of the inter-root soil microbial community associated with Iris tectorum under greenhouse pot conditions.The results demonstrated significant changes in the diversity and composition of the inter-root soil microbial community following AMF inoculation,with Proteobacteria,Actinobacteria,Chloroflexi,Acidobacteria,and Bacteroidetes being the dominant taxa.Under Cr stress,species and gene co-occurrence network analysis revealed that AMF promoted the transformation process of organic phosphorus mineralization and facilitated inorganic phosphorus uptake.Additionally,network analysis of functional genes indicated strong aggregation of(pstS,pstA,pstC,TC.PIT,phoR,pp-gppA)genes,which collectively enhanced phosphorus uptake by plants.These findings shed light on the inter-root soil phosphorus cycling process during the co-remediation of Cr-contaminated soil by AMF-Iris tectorum symbiosis,providing valuable theoretical support for the application of AMF-wetland plant symbiosis systems to remediate heavy metal-contaminated soil.
基金Supported by the National Natural Science Foundation of China(No.82371093,No.72342015)R&D Program of Beijing Municipal Education Commission(No.KZ202110025039).
文摘AIM:To compare the efficacy of different administration regimens of compound tropicamide eyedrops(CTE)for pupil dilation for children with dark iris.METHODS:A prospective,comparative,randomized interventional study was conducted.Children in Group 1 received CTE 3 times with a 3min interval between each application.Children in Group 2 received CTE 4 times with a 5min interval between each application.We measured their pupil diameters at baseline(pre-drug instillation)and 30min and 60min post-drug instillation and assessed the pupillary light reflex at 60min post-drug instillation.RESULTS:In total,194 eyes of 101 children were enrolled.The changes of pupil diameter at 30min and 60min post-drug instillation were 1.2±0.6 mm and 2.3±1.0 mm in Group 1,and 2.3±0.9 mm and 3.7±1.0 mm in Group 2,respectively.Group 2 showed a larger change in pupil size than Group 1 at 30min(P<0.01)and 60min(P<0.01).The effect of pupil dilation in Group 2 was 1.25 times that in Group 1.The change in pupil size was positively associated with age.A higher proportion of children in Group 1 had smaller pupil diameter and reactive pupils at the final time point,with only 33 children(33.7%)had final pupil size≥6.5 mm,and only 9 children(9.2%)had non-reactive pupils.Children in Group 2 achieved larger pupil diameter and more nonreactive pupils at the final time point,with 84 children(87.5%)had final pupil size≥6.5 mm,and only 22 children(22.9%)had reactive pupils.CONCLUSION:Increasing the frequency of compound tropicamide and lengthening the interval between eye drop applications can produce stronger mydriatic effects.
基金supported by the Science and Technology Basic Resources Investigation Program of China(2019FY100500)the Fun-damental Research Funds for the Central Universities(2572023CT18)the Natural Fund Project of Heilongjiang Province(LH 2020C 044).
文摘In northern China,light and temperature are major limiting factors for plant growth,particularly during seed production and seedling establishment.While previous studies suggested a possible role for the MYB97 gene in cold-stress,confirmation through documented evidence was lacking.In this study,we transformed the MYB97 gene from Iris laevigata into tobacco,and discovered that the gene boosted photosynthesis,photoprotection and resilience to cold.The transgenic tobacco seeds exhibited enhanced germination and accelerated seedling growth.Moreover,these plants had decreased levels of MDA(Malondialdehyde)and relative conductance,coupled with elevated concentrations of proline and soluble sugars.This response was accompanied by heightened activity of antioxidant enzymes during periods of cold stress(4 and−2℃).Exposure to low temperatures(0–15℃)also reduced heights but accentuated primary root growth in transgenic tobacco plants.Additionally,tobacco leaves showed an increased growth along with higher chlorophyll levels,net photosynthetic rates,stomatal conductance,transpiration rates and non-photochemical quenching coefficient.This study shows that IlMYB97(The MYB97 genes in I.laevigata)improves cold-resistance,and enhances photosynthesis and photoprotective ability,and thus overall growth and development.These findings would offer the genetic resources to further study cold resistance and photosynthesis.
基金supported by the National Science Foundation(Grant No.31670344)the Fundamental Research Funds for the Central Universities(Grant No.2572023CT18)the Natural Fund Project of Heilongjiang Province(Grant No.LH2020C044).
文摘Iris sanguinea Donn ex Horn.is a common perennial ornamental herb in Northeast China due to its strong cold-resistance capacity.In this study,integrative analyses of metabolomics and transcriptomics data were performed on five cultivars of I.sanguinea with varying petal colors to elucidate the molecular basis underlying petal pigmentation.The metabolomics data revealed that a total of five major anthocyanins were responsible for petal pigmentation in I.sanguinea.While two delphinidin-3-O-glucoside derivatives contributed to blue coloration in petals,two petunidin-3-O-glucoside and cyanidin-3-O-rutinoside derivatives exhibited significantly higher abundances in the deep pink petals.In addition,transcriptomics revealed a gradual increase in the expression of a large set of genes involved in flavonoid and anthocyanin biosynthesis pathways,corresponding to the varying levels of anthocyanin content among cultivar petals.Integrated metabolomics and transcriptomics analysis further led to the discovery of molecular modules controlling petal pigmentation,in which an R2R3 MYB transcription factor IsMYBL1 was found to be associated with the accumulation of key anthocyanin derivatives,it localized to the nucleus.The regulatory role of IsMYBL1 in anthocyanin accumulation has been validated through overexpressing in tobacco and knockdown in I.sanguinea using an improved virusinduced gene silencing(VIGS)approach.Finally,BiFC and Y2H showed that IsMYBL1 interacts with IsEGL3 and IsTTG1 to activate the expression of genes involved in anthocyanin biosynthesis,Yeast one-hybrid,dual-luciferase assays,showed that IsMYBL1 significantly increased anthocyanin contents in I.sanguinea by promoting the expression of IsANS,thereby promoting anthocyanin accumulation.Taken together,our study has provided a comprehensive molecular profiling of petal coloration in I.sanguinea and revealed key gene/metabolite networks that determine petal pigmentation.
基金Key Research and Development Projects of Zhejiang Science and Technology Plan(No.2021C03103)Research and Development Plan of Zhejiang Science and Technology Department(No.2023C03089)Scientific Research Project of Zhejiang Provincial Department of Education(No.Y202456401)。
文摘Phakic intraocular lens implantation has become one of the important means of correcting refractive errors today.Among them,the implantable collamer lens(ICL)is favored for its wide range of correction,excellent optical quality,and high safety,but the risks of postoperative complications such as glaucoma and anterior subcapsular opacification still exist.Vault is an important indicator for evaluating the safety after ICL implantation,and its ideal state is crucial for preventing complications.Studies have shown that iris morphology has a significant impact on vault.In order to further optimize surgical outcomes and improve surgical safety,this review comprehensively reviews the research progress of iris-related parameters in ICL implantation and discusses the importance of various parameters in preoperative evaluation and postoperative follow-up.
基金supported by the National Key Research and Development Program of China(Grant No.2021YFF0502900)the National Natural Science Foundation of China(Grant Nos.62575066 and 62027824)+3 种基金the Guangdong Basic and Applied Basic Research Foundation(Grant No.2024A1515011344)the Innovation and Entrepreneurship Teams Project of Guangdong Pearl River Talents Program(Grant No.2019ZT08Y105)the Guangdong-Hong Kong-Macao Intelligent Micro-Nano Optoelectronic Technology Joint Laboratory(Grant No.2020B1212030010)the National Institutes of Health/National Eye Institute(NIH/NEI)(Grant Nos.P30EY07551,R01EY022362,and R01EY022362).
文摘In vivo imaging of human iris vasculature remains a persistent challenge,limiting our understanding of its relationship with ocular disease pathogenesis.Conventional raster scan optical coherence tomography angiography(OCTA)suffers from angular-dependent contrast(including blind spots),limited field of view,and prolonged imaging time—challenges that restrict its clinical utility.We introduce a circular interleaving scan OCTA method that overcomes these barriers by enabling 360 deg high-contrast iris angiography with consistent spatiotemporal sampling and optimized motion contrast.The circular scan design enables directionoptimized sampling:we configured circumferential sampling density to approximately twice the radial density,enhancing detection of radially oriented iris vasculature.A Cartesian–polar coordinate transformation was implemented for eye-motion compensation,vessel realignment,and vasculature reconstruction.Compared with raster scan OCTA,our circular scan protocol demonstrates 1.55×higher efficiency in iris vascular imaging,featuring a superior duty cycle(99.95%versus 82.00%)and eliminating redundant data acquisition from rectangular field corners(27.3%of the circular area).This method improves vessel density measurement by 39.0%and vessel count quantification by 25.2%relative to raster scans.By eliminating angular-dependent blind spots,our method significantly enhances vascular quantification reliability,paving the way to a better understanding of ocular diseases and holding promising potential for future clinical applications.
