A new iris feature extraction approach using both spatial and frequency domain is presented. Steerable pyramid is adopted to get the orientation information on iris images. The feature sequence is extracted on each su...A new iris feature extraction approach using both spatial and frequency domain is presented. Steerable pyramid is adopted to get the orientation information on iris images. The feature sequence is extracted on each sub-image and used to train Support Vector Machine (SVM) as iris classifiers. SVM has drawn great interest recently as one of the best classifiers in machine learning, although there is a problem in the use of traditional SVM for iris recognition. It cannot treat False Accept and False Reject differently with different security requirements. Therefore, a new kind of SVM called Non-symmetrical SVM is presented to classify the iris features. Experimental data shows that Non-symmetrical SVM can satisfy various security requirements in iris recognition applications. Feature sequence combined with spatial and frequency domain represents the variation details of the iris patterns properly. The results in this study demonstrate the potential of our new approach, and show that it performs more satis- factorily when compared to former algorithms.展开更多
Research on somacolonal variation in hexaploid wheat (Triticum aestivum L.) has been conducted a lot and morphological, cytological, and biochemical variations have been observed. Evidence for gene mutations has been ...Research on somacolonal variation in hexaploid wheat (Triticum aestivum L.) has been conducted a lot and morphological, cytological, and biochemical variations have been observed. Evidence for gene mutations has been obtained through the analysis of gliadin and glutenin in wheat grain, but loci of mutations have not been located. The aims of this study are: firstly, to detect mutation loci of specific gene (Glu-1) to provide exact evi-dence for gene mutation by analyzing changes of high-molecular-weight glutenin展开更多
On the basis of previous studies dealing with the variation of major agronomic and yield characteristics of regenerated plants derived from single cell culture in vitro of common wheat (Triticum aestivum L.Cult...On the basis of previous studies dealing with the variation of major agronomic and yield characteristics of regenerated plants derived from single cell culture in vitro of common wheat (Triticum aestivum L.Cultivar NE 7742), the grain protein content and its fractions from regenerated plants with stable agronomic characteristics were studied from 1992 to 1995. The results showed that the variation of grain protein content and its fractions in somaclones from single cell culture in vitro were very significant and the range was very wide (11531770%). Several types of variation were found in the studies, especially the type with higher protein content than that of cultivar NE 7742 (non-culture parent). Among them, -2069% of lines the grain protein content was significantly higher than that of NE 7742 and combined with high yielding potential. The tendency of variation of the four protein fractions showed that the variation of albumin was not obvious and maintained the same level as NE774 increased in some somaclones and decreased in others. However, the percentages both globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and albumm was mainly influenced by globulin under the condition of culture in vitro. The variation of total amount of storage protein and the ratio between gliadin and glutenin was mainly affected by glutenin. The results mentioned above demonstrated that the induction and screening of somaclonal variation could be an effective way in wheat improvement in combining high protein content with high yield.展开更多
Somaclonal variation of calli and regenerated plants of spring wheat were detected by using technique RAPD in the study. Calli at different culture stages and regenerated plants derived from young spikes and immature ...Somaclonal variation of calli and regenerated plants of spring wheat were detected by using technique RAPD in the study. Calli at different culture stages and regenerated plants derived from young spikes and immature embryos were used as materials. Molecular variation could be reflected from electrophoresis pattern of RAPD fragments at different culture stage in calli, and in regenerated plants derived from different explants, even no phenotype variations were found. Somaclonal variation in calli and in regenerated plants appeared regularly: A higher frequency of variation in hybrids F2 was detected than that of the cultivar that is stable genetically. High variation frequency of RAPD fragments appeared in calli when cultured 75 days. The identical variations of RAPD fragments were observed in calli and in the regenerated plants induced from different genotype or explants. The variation frequency detected is higher in regenerated plants than that of in calli. RAPD could be applied easily and simply to determine variation in level of DNA at each stage cultured in vitro.展开更多
The genetic stability of variations of main agronomic characteristics in progenies from the regenerated plants of immature embryo culture in vitro was studied. The results showed that the variations of earliness in ma...The genetic stability of variations of main agronomic characteristics in progenies from the regenerated plants of immature embryo culture in vitro was studied. The results showed that the variations of earliness in maturity, high 1000-grain weight, plant height and grain weight per spike were heritable and tended to be stable in IE3 and completely stable in IE4 The wide variation of main agronomic characteristics induced by embryo culture in vitro provided the probability of selection in wheat improvement. Some somaclonal lines with useful variations could be used directly in wheat production.展开更多
Adventive regenerants of Populus × berolinensis Dipp. were obtained on 1/2 MS salts with sucrose, vitamins, BA, TDZ and NAA using stem, petiole and leaf explants. They differed from each other in plantlet dimensi...Adventive regenerants of Populus × berolinensis Dipp. were obtained on 1/2 MS salts with sucrose, vitamins, BA, TDZ and NAA using stem, petiole and leaf explants. They differed from each other in plantlet dimensions. More than 50 clones were produced from initial regenerants by excision and rooting of apexes and lateral shoots. Differences in stem length and thickness were observed between 200 field plants produced from in vitro plantlets. Differences in stem length were conditioned mainly due to different number of internodes and not by internodes’ lengths. Plants grown from cuttings excised from highest and smallest plants retained the abilities of mother plants to grow with different rates. It is concluded that somaclonal variability can be used for selection of fast growing poplar plants which are necessary for industrial plantations. These plants can be used for this purpose without the limitations existing for transgenic plants.展开更多
Determination of the somaclonal variation of in vitro-propagated plants is crucial to determine the appropriate micropropagation protocol and growth regulators for commercial scale multiplication.In this research,nine...Determination of the somaclonal variation of in vitro-propagated plants is crucial to determine the appropriate micropropagation protocol and growth regulators for commercial scale multiplication.In this research,nine multiplication media(MM)augmented with different concentrations of 6-benzyl adenine(BA),Kinetin(Kin),and Thidiazuron(TDZ),Three rooting media(RM)supplemented with three levels ofα-naphthalene acetic acid(NAA)and three types of soil mixtures(v/v);Coco peat/Vermiculite/Sand(CVS),Peat moss/Perlite/Sand(PPS)and Peat moss/Perlite(PP)were used in the micropropagation protocol of daylily plants.MM2 showed the maximum shoot length and the number of leaves,while MM9 showed the maximum number of shoots.The RM1 showed the maximum root length and the number of roots.During acclimatization,CVS,PPS,and PP soil mixture showed similar performance except the CVS mixture showed lower performance regarding plant height and diameter.The genetic fidelity of micropropagated plants was evaluated using Start Codon Targeted(SCoT)Markers.Six SCoT primers amplified 51 scorable bands with an approximate range from 146 bp to 1598 bp size.Thirty one out of 51 loci were presented in the mother plants.40 loci were polymorphic,11 were monomorphic and 7 were unique.The amplification patterns of the micropropagated plants demonstrated genetic integrity to the mother plant ranging from 84.32 to 47.06 and somaclonal variations ranging from 52.94 with 5 mg/l BA pathway to 15.68 with 1mg/l TDZ pathway,thus demonstrating that the homogeneity and the variation of the micropropagated plants affected by the type and the quantity of the plant growth regulator used during multiplication subcultures.This research can be successfully used for other ornamental and medicinal plants’bulk multiplication,germplasm conservation,and future genetic improvement.展开更多
Yield characters, maturity and grain protein content of somaclones derived both from immature embryo of cultivar 77(2)-Spring and single-cell culture of cultivar NE7742 in vitro were studied and the wide variation was...Yield characters, maturity and grain protein content of somaclones derived both from immature embryo of cultivar 77(2)-Spring and single-cell culture of cultivar NE7742 in vitro were studied and the wide variation was found. Somaclones with maturity 8 days earlier than or the same as CK NE 7742 (high yield, early maturity and high quality), combining with high quality (grain protein content 15.5% - 18%) and high yield (the same as 7724 or higher) have been found and selected and now multiplied for 8 generations. The results of cultivar comparison trial in 1995 showed that several somaclones (the yields were significantly higher than CK DN120) could be used directly in wheat production. The studies confirmed that somaclonal variation is one of the effective ways for early maturity, high-yielding and high-quality improvement in wheat.展开更多
Calli have been widely used as research materials for clonal propagation and transformation of trees owing to their high multiplication rates and capacity of large-scale propagation. Till now, very little is known on ...Calli have been widely used as research materials for clonal propagation and transformation of trees owing to their high multiplication rates and capacity of large-scale propagation. Till now, very little is known on the soma-展开更多
Rice male sterile (MS) lines, IR66707A and IR69700A, which possess the cytoplasm of Oryza perennis and O. glumaepatula respectively, belong to the cytoplasmic type. Their sterility could be maintained but not be resto...Rice male sterile (MS) lines, IR66707A and IR69700A, which possess the cytoplasm of Oryza perennis and O. glumaepatula respectively, belong to the cytoplasmic type. Their sterility could be maintained but not be restored. By somatic cell culture of these two MS lines, 47 somaclones with 465 R1 plants were obtained. All of the 465 R1 plants were sterile in the spring season in Guangzhou. According to the expression of the R1 plants and the level of similarity to their donor parents, they could be divided into three types. The plants of type I were male sterile. The sterility of some somaclones of this type could be restored by the test crossing varieties or alternated to fertile by changes of some environmental conditions. The hybrid F1 of test cross from the MS somaclones in type I was fertile while the hybrid F1 from the donor MS lines was still sterile. The R1 plants of type Ⅱ were similar to the donor parents and also male sterile. The hybrid F1 from all of the plants of type Ⅱ crossed to test展开更多
Wheat is a crucial crop for global food security,and effective in vitro plant regeneration techniques are considered a precondition for genetic engineering in wheat breeding programs.A practical approach for in vitro ...Wheat is a crucial crop for global food security,and effective in vitro plant regeneration techniques are considered a precondition for genetic engineering in wheat breeding programs.A practical approach for in vitro regeneration of the Kirik bread wheat cultivar via somatic embryogenesis was investigated using endospermsupported mature embryos.Callus cultures were initiated from mature embryos supported by endosperm,cultured on phytagel-based Murashige and Skoog(MS)basal mediumcontaining dicamba(12mg/L)and indole-3-acetic acid(IAA)(0.5 mg/L)under dark conditions.This research was designed to examine the impact of putrescine(Put)(0.0 and 1.0 mM)on inducing embryonic callus and the effects of thidiazuron(TDZ)(0.0,0.1,0.2,0.3,0.4,and 0.5 mg/L)on wheat regeneration.Adding 1.0mM putrescine to MS mediumsignificantly increased(p<0.01)embryogenic callus formation,resulting in a complete(100%)induction rate.Moreover,the highest number of regenerated plants per explant(5.8)was obtained through the synergistic interaction between 1.0 mM putrescine and 0.5 mg/L TDZ.To assess the genetic homogeneity of regenerated plants,10 different inter-simple sequence repeat(ISSR)primers were utilized,revealing a high level of genetic stability.The results of all the applications of a particular plant tissue culture technique showed a level of somaclonal variation within acceptable limits,indicating that the genetic diversity of the plant populations was protectedwithout compromising the desired traits.These improvements offer a promising tool forwheat biotechnology,especially for genetic transformation.展开更多
Tissue culture is one of the most promising and practical methods for conserving endangered plant species.Therefore,the present study evaluates the conservation of the endangered seaweed Sargassum fusiforme through ti...Tissue culture is one of the most promising and practical methods for conserving endangered plant species.Therefore,the present study evaluates the conservation of the endangered seaweed Sargassum fusiforme through tissue culturing techniques from different explants for the first time.Besides,the genetic variation of the mother plant and cultivated explants using inter-simple sequence repeat(ISSR)techniques;this methodology was the first to be illustrated in such work for algae tissue culturing.The regeneration results have shown that different explants could induce shoot and rhizoid morphogenesis with a total number of blades of 2436,1011,1466,678,and 6 from apical parts,stipe with blades,two-segmented seedlings,rhizoids,and stipe without blades,respectively.The total length was 234,181.8,83.5,and 81.8 cm from the two-segmented seedlings,apical parts,stipe without blades,and stipe with blades,respectively.At the same time,the total wet weight was 73.148,48.369,35.731,18.588,and 2.035 g from the apical parts,the two-segmented seedlings,stipe with blades,rhizoids,and stipe without blades,respectively.Micropropagation of S.fusiforme was successfully achieved with apical,stipe,and stolon segments using free PES media.It is suggested that the applied genetic fingerprint is valid for S.fusiforme and will respond well to molecular marker assistance in cultivation.The significance of S.fusiforme and its exposure to being endangered due to over-exploitation have made its regeneration in vitro a subject of interest in this study.Thus,this report represents the successful regeneration of S.fusiforme and explores the genetic uniformity or somaclonal variation of the obtained seedlings using the ISSR-PCR marker for the first time.展开更多
[Objective]The aim was to study the sensitive response characteristics of the main agronomic traits of somaclonal and the variation of sensitive response of the lines of somaclonal to sowing date. [Method]The RITSD ch...[Objective]The aim was to study the sensitive response characteristics of the main agronomic traits of somaclonal and the variation of sensitive response of the lines of somaclonal to sowing date. [Method]The RITSD changing of agronomic traits was studied by using 24 rice somaclonal and its donor parent under two sowing dates. [Result]The average values of RITSD were higher （over one） for the traits of pant height （PH）,density of spikelets （DS）,filled spikelets per panicle （FSP）,spikelets per panicle （SP） and grain weight per plant （GWP）,but lower for days of sowing to heading （DSH）,effective panicles （EP）,panicle length （PL）,seed setting rate （SS） and 1 000-grain weight （1 000-GW）. These showed that PH,DS,FSP,SP and GWP were easily affected by sowing date and tended to higher with the later sowing; the RITSD of somaclonal changed with the agronomic traits. The frequency of RITSD＇ variation of DSH and PH was higher than that of PL. The RITSD＇ value of EP,PL,DS,FSP,SP and SS became higher,and higher or lower for DSH and GWP in different somaclonal; compared with the donor,20 of 24 was significantly different,and the frequency of RITSD variation was over 83. 3%,however,RITSD of the most lines （75%） changed only for 1-2 characters; cluster analysis showed that the RITSD of agronomic traits belonged to different combinations of somaclonal in rice. [Conclusion]the study had provided a theoretical basis for the screening and using of the somaclonal.展开更多
基金Project supported by the National Natural Science Foundation of China (No. 60272031), Educational Department Doctor Foundation of China (No. 20010335049), and Zhejiang Provincial Natural ScienceFoundation (No. ZD0212), China
文摘A new iris feature extraction approach using both spatial and frequency domain is presented. Steerable pyramid is adopted to get the orientation information on iris images. The feature sequence is extracted on each sub-image and used to train Support Vector Machine (SVM) as iris classifiers. SVM has drawn great interest recently as one of the best classifiers in machine learning, although there is a problem in the use of traditional SVM for iris recognition. It cannot treat False Accept and False Reject differently with different security requirements. Therefore, a new kind of SVM called Non-symmetrical SVM is presented to classify the iris features. Experimental data shows that Non-symmetrical SVM can satisfy various security requirements in iris recognition applications. Feature sequence combined with spatial and frequency domain represents the variation details of the iris patterns properly. The results in this study demonstrate the potential of our new approach, and show that it performs more satis- factorily when compared to former algorithms.
基金State Key Laboratory of Plant Cell and Genetic Engineering.
文摘Research on somacolonal variation in hexaploid wheat (Triticum aestivum L.) has been conducted a lot and morphological, cytological, and biochemical variations have been observed. Evidence for gene mutations has been obtained through the analysis of gliadin and glutenin in wheat grain, but loci of mutations have not been located. The aims of this study are: firstly, to detect mutation loci of specific gene (Glu-1) to provide exact evi-dence for gene mutation by analyzing changes of high-molecular-weight glutenin
文摘On the basis of previous studies dealing with the variation of major agronomic and yield characteristics of regenerated plants derived from single cell culture in vitro of common wheat (Triticum aestivum L.Cultivar NE 7742), the grain protein content and its fractions from regenerated plants with stable agronomic characteristics were studied from 1992 to 1995. The results showed that the variation of grain protein content and its fractions in somaclones from single cell culture in vitro were very significant and the range was very wide (11531770%). Several types of variation were found in the studies, especially the type with higher protein content than that of cultivar NE 7742 (non-culture parent). Among them, -2069% of lines the grain protein content was significantly higher than that of NE 7742 and combined with high yielding potential. The tendency of variation of the four protein fractions showed that the variation of albumin was not obvious and maintained the same level as NE774 increased in some somaclones and decreased in others. However, the percentages both globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and albumm was mainly influenced by globulin under the condition of culture in vitro. The variation of total amount of storage protein and the ratio between gliadin and glutenin was mainly affected by glutenin. The results mentioned above demonstrated that the induction and screening of somaclonal variation could be an effective way in wheat improvement in combining high protein content with high yield.
文摘Somaclonal variation of calli and regenerated plants of spring wheat were detected by using technique RAPD in the study. Calli at different culture stages and regenerated plants derived from young spikes and immature embryos were used as materials. Molecular variation could be reflected from electrophoresis pattern of RAPD fragments at different culture stage in calli, and in regenerated plants derived from different explants, even no phenotype variations were found. Somaclonal variation in calli and in regenerated plants appeared regularly: A higher frequency of variation in hybrids F2 was detected than that of the cultivar that is stable genetically. High variation frequency of RAPD fragments appeared in calli when cultured 75 days. The identical variations of RAPD fragments were observed in calli and in the regenerated plants induced from different genotype or explants. The variation frequency detected is higher in regenerated plants than that of in calli. RAPD could be applied easily and simply to determine variation in level of DNA at each stage cultured in vitro.
文摘The genetic stability of variations of main agronomic characteristics in progenies from the regenerated plants of immature embryo culture in vitro was studied. The results showed that the variations of earliness in maturity, high 1000-grain weight, plant height and grain weight per spike were heritable and tended to be stable in IE3 and completely stable in IE4 The wide variation of main agronomic characteristics induced by embryo culture in vitro provided the probability of selection in wheat improvement. Some somaclonal lines with useful variations could be used directly in wheat production.
文摘Adventive regenerants of Populus × berolinensis Dipp. were obtained on 1/2 MS salts with sucrose, vitamins, BA, TDZ and NAA using stem, petiole and leaf explants. They differed from each other in plantlet dimensions. More than 50 clones were produced from initial regenerants by excision and rooting of apexes and lateral shoots. Differences in stem length and thickness were observed between 200 field plants produced from in vitro plantlets. Differences in stem length were conditioned mainly due to different number of internodes and not by internodes’ lengths. Plants grown from cuttings excised from highest and smallest plants retained the abilities of mother plants to grow with different rates. It is concluded that somaclonal variability can be used for selection of fast growing poplar plants which are necessary for industrial plantations. These plants can be used for this purpose without the limitations existing for transgenic plants.
基金supported by the Deanship of Scientific Research,Vice Presidency for Graduate Studies and Scientific Research,King Faisal University,Saudi Arabia[Project No.GRANT3039]the Princess Nourah bint Abdulrahman University Researchers Supporting Project Number(PNURSP2023R318),Princess Nourah bint Abdulrahman University,Riyadh,Saudi Arabia.
文摘Determination of the somaclonal variation of in vitro-propagated plants is crucial to determine the appropriate micropropagation protocol and growth regulators for commercial scale multiplication.In this research,nine multiplication media(MM)augmented with different concentrations of 6-benzyl adenine(BA),Kinetin(Kin),and Thidiazuron(TDZ),Three rooting media(RM)supplemented with three levels ofα-naphthalene acetic acid(NAA)and three types of soil mixtures(v/v);Coco peat/Vermiculite/Sand(CVS),Peat moss/Perlite/Sand(PPS)and Peat moss/Perlite(PP)were used in the micropropagation protocol of daylily plants.MM2 showed the maximum shoot length and the number of leaves,while MM9 showed the maximum number of shoots.The RM1 showed the maximum root length and the number of roots.During acclimatization,CVS,PPS,and PP soil mixture showed similar performance except the CVS mixture showed lower performance regarding plant height and diameter.The genetic fidelity of micropropagated plants was evaluated using Start Codon Targeted(SCoT)Markers.Six SCoT primers amplified 51 scorable bands with an approximate range from 146 bp to 1598 bp size.Thirty one out of 51 loci were presented in the mother plants.40 loci were polymorphic,11 were monomorphic and 7 were unique.The amplification patterns of the micropropagated plants demonstrated genetic integrity to the mother plant ranging from 84.32 to 47.06 and somaclonal variations ranging from 52.94 with 5 mg/l BA pathway to 15.68 with 1mg/l TDZ pathway,thus demonstrating that the homogeneity and the variation of the micropropagated plants affected by the type and the quantity of the plant growth regulator used during multiplication subcultures.This research can be successfully used for other ornamental and medicinal plants’bulk multiplication,germplasm conservation,and future genetic improvement.
文摘Yield characters, maturity and grain protein content of somaclones derived both from immature embryo of cultivar 77(2)-Spring and single-cell culture of cultivar NE7742 in vitro were studied and the wide variation was found. Somaclones with maturity 8 days earlier than or the same as CK NE 7742 (high yield, early maturity and high quality), combining with high quality (grain protein content 15.5% - 18%) and high yield (the same as 7724 or higher) have been found and selected and now multiplied for 8 generations. The results of cultivar comparison trial in 1995 showed that several somaclones (the yields were significantly higher than CK DN120) could be used directly in wheat production. The studies confirmed that somaclonal variation is one of the effective ways for early maturity, high-yielding and high-quality improvement in wheat.
基金grants （C0212） from Natural Science Foundation of Heilongjiang Provenience.
文摘Calli have been widely used as research materials for clonal propagation and transformation of trees owing to their high multiplication rates and capacity of large-scale propagation. Till now, very little is known on the soma-
文摘Rice male sterile (MS) lines, IR66707A and IR69700A, which possess the cytoplasm of Oryza perennis and O. glumaepatula respectively, belong to the cytoplasmic type. Their sterility could be maintained but not be restored. By somatic cell culture of these two MS lines, 47 somaclones with 465 R1 plants were obtained. All of the 465 R1 plants were sterile in the spring season in Guangzhou. According to the expression of the R1 plants and the level of similarity to their donor parents, they could be divided into three types. The plants of type I were male sterile. The sterility of some somaclones of this type could be restored by the test crossing varieties or alternated to fertile by changes of some environmental conditions. The hybrid F1 of test cross from the MS somaclones in type I was fertile while the hybrid F1 from the donor MS lines was still sterile. The R1 plants of type Ⅱ were similar to the donor parents and also male sterile. The hybrid F1 from all of the plants of type Ⅱ crossed to test
文摘Wheat is a crucial crop for global food security,and effective in vitro plant regeneration techniques are considered a precondition for genetic engineering in wheat breeding programs.A practical approach for in vitro regeneration of the Kirik bread wheat cultivar via somatic embryogenesis was investigated using endospermsupported mature embryos.Callus cultures were initiated from mature embryos supported by endosperm,cultured on phytagel-based Murashige and Skoog(MS)basal mediumcontaining dicamba(12mg/L)and indole-3-acetic acid(IAA)(0.5 mg/L)under dark conditions.This research was designed to examine the impact of putrescine(Put)(0.0 and 1.0 mM)on inducing embryonic callus and the effects of thidiazuron(TDZ)(0.0,0.1,0.2,0.3,0.4,and 0.5 mg/L)on wheat regeneration.Adding 1.0mM putrescine to MS mediumsignificantly increased(p<0.01)embryogenic callus formation,resulting in a complete(100%)induction rate.Moreover,the highest number of regenerated plants per explant(5.8)was obtained through the synergistic interaction between 1.0 mM putrescine and 0.5 mg/L TDZ.To assess the genetic homogeneity of regenerated plants,10 different inter-simple sequence repeat(ISSR)primers were utilized,revealing a high level of genetic stability.The results of all the applications of a particular plant tissue culture technique showed a level of somaclonal variation within acceptable limits,indicating that the genetic diversity of the plant populations was protectedwithout compromising the desired traits.These improvements offer a promising tool forwheat biotechnology,especially for genetic transformation.
基金supported by the grants from China Asia-Pacific Economic Cooperation(Nos.HV180101 and HV01-190101)the Xiamen Science and Technology Program(No.3502Z20161162)the Specific Fund Project of Xiamen Marine and Fishery Development(No.19CZP011HJ08).
文摘Tissue culture is one of the most promising and practical methods for conserving endangered plant species.Therefore,the present study evaluates the conservation of the endangered seaweed Sargassum fusiforme through tissue culturing techniques from different explants for the first time.Besides,the genetic variation of the mother plant and cultivated explants using inter-simple sequence repeat(ISSR)techniques;this methodology was the first to be illustrated in such work for algae tissue culturing.The regeneration results have shown that different explants could induce shoot and rhizoid morphogenesis with a total number of blades of 2436,1011,1466,678,and 6 from apical parts,stipe with blades,two-segmented seedlings,rhizoids,and stipe without blades,respectively.The total length was 234,181.8,83.5,and 81.8 cm from the two-segmented seedlings,apical parts,stipe without blades,and stipe with blades,respectively.At the same time,the total wet weight was 73.148,48.369,35.731,18.588,and 2.035 g from the apical parts,the two-segmented seedlings,stipe with blades,rhizoids,and stipe without blades,respectively.Micropropagation of S.fusiforme was successfully achieved with apical,stipe,and stolon segments using free PES media.It is suggested that the applied genetic fingerprint is valid for S.fusiforme and will respond well to molecular marker assistance in cultivation.The significance of S.fusiforme and its exposure to being endangered due to over-exploitation have made its regeneration in vitro a subject of interest in this study.Thus,this report represents the successful regeneration of S.fusiforme and explores the genetic uniformity or somaclonal variation of the obtained seedlings using the ISSR-PCR marker for the first time.
基金Supported by Chongqing Major Research Project "Innovation in Hybrid Rice Breeding and New Materials" ( CSTC, 2007, AA1019 )Sichuan Province Breeding Research Project (06yzgg-01)~~
文摘[Objective]The aim was to study the sensitive response characteristics of the main agronomic traits of somaclonal and the variation of sensitive response of the lines of somaclonal to sowing date. [Method]The RITSD changing of agronomic traits was studied by using 24 rice somaclonal and its donor parent under two sowing dates. [Result]The average values of RITSD were higher （over one） for the traits of pant height （PH）,density of spikelets （DS）,filled spikelets per panicle （FSP）,spikelets per panicle （SP） and grain weight per plant （GWP）,but lower for days of sowing to heading （DSH）,effective panicles （EP）,panicle length （PL）,seed setting rate （SS） and 1 000-grain weight （1 000-GW）. These showed that PH,DS,FSP,SP and GWP were easily affected by sowing date and tended to higher with the later sowing; the RITSD of somaclonal changed with the agronomic traits. The frequency of RITSD＇ variation of DSH and PH was higher than that of PL. The RITSD＇ value of EP,PL,DS,FSP,SP and SS became higher,and higher or lower for DSH and GWP in different somaclonal; compared with the donor,20 of 24 was significantly different,and the frequency of RITSD variation was over 83. 3%,however,RITSD of the most lines （75%） changed only for 1-2 characters; cluster analysis showed that the RITSD of agronomic traits belonged to different combinations of somaclonal in rice. [Conclusion]the study had provided a theoretical basis for the screening and using of the somaclonal.
