This paper provides a systematic review of Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging(MALDI-MSI),encompassing its technical principles,experimental workflows,matrix optimization strategies,a...This paper provides a systematic review of Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging(MALDI-MSI),encompassing its technical principles,experimental workflows,matrix optimization strategies,and recent advancements in plant science applications.It highlights the method's groundbreaking applications in spatial mapping of plant metabolites,dynamic hormone monitoring,and functional studies of tissue microdomains,while offering critical insights into current technical limitations and future research directions.展开更多
In this paper, a sensitive and specific liquid chromatography-electrospray ionisation-mass spectrometry （LC-ESI-MS） method has been developed and validated for the identification and determination of seven flavonoid...In this paper, a sensitive and specific liquid chromatography-electrospray ionisation-mass spectrometry （LC-ESI-MS） method has been developed and validated for the identification and determination of seven flavonoids, namely epimedin A, epimedin B, epimedin C, icariin, sagittatoside B, 2＂-O-rhamnosyl icariside II, and baohuoside I in Epimedium from different sources.展开更多
In this study,protein from Porphyra haitanensis was used as raw material to prepare an antioxidant peptide,and its antioxidant activity was evaluated in vitro.A model of H_2O_2-induced oxidative damage in Hep G2 cells...In this study,protein from Porphyra haitanensis was used as raw material to prepare an antioxidant peptide,and its antioxidant activity was evaluated in vitro.A model of H_2O_2-induced oxidative damage in Hep G2 cells was established,and the effects of Porphyra haitanensis hydrolysates (PHHs) on superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were detected.Finally,the structure of PHHs was identified by ESI-MS/MS.The results showed that the 1,1-diphenyl-2-pyridylhydrazine(DPPH)-free radical-scavenging ability of PHHs was the strongest (59.28%at 1.0 mg m L~(-1)) when hydrolyzed with an acidic protease for 4 h.PHHs with different concentrations had protective effects on H_2O_2-induced damage to Hep G2 cells,and the protective effect was enhanced with increasing concentrations.When the level was 400μg m L~(-1),the cell survival rate was as high as 88.62%.Moreover,PHHs can significantly reduce oxidative damage to Hep G2 cells by H_2O_2,improve SOD activity,and reduce MDA content.The tetrapeptide Asp-Lys-Ser-Thr,with a molecular weight of 448 Da,was identified as an important fraction of PHHs by high-resolution mass spectrometry.展开更多
This study focused,for the first time,on the effect of ultrasonic features on the extraction efficiency of secondary metabolites in mustard seed cake(MSC).The nematostatic potential of sonicated seed cake was examined...This study focused,for the first time,on the effect of ultrasonic features on the extraction efficiency of secondary metabolites in mustard seed cake(MSC).The nematostatic potential of sonicated seed cake was examined against the second-stage juveniles(J2s)of root-knot nematode,Meloidogyne javanica.The results show that a 35 ppm(parts per million)concentration of a sonicated extract(SE)sample of MSC caused 65%J2s mortality at 18 h exposure period in vitro.It also significantly suppressed the root-knot index(RKI=0.94)in tomato roots.The lethal concentration values for SE were 51.76,29.79,and 13.34 ppm,respectively,at 6,12,and 18 h of the exposure period,and the lethal concentration values for the non-sonicated extract(NSE)sample were 116.95,76.38,and 55.59 ppm,respectively,at similar exposure time.Sinapine and gluconapin were identified as the major compounds in ultrasonic-assisted MSC.Because of the high extraction efficiency of metabolites in the SE,all treat‐ments of SE were shown to be antagonistic to J2s.Thus,this study of ultrasonication activity-based profiling of MSC may help generate target-based compounds at a scale relevant to the control of disease caused by nematodes in economic crops.展开更多
Objective:To evaluate antioxidant,cytotoxic,and anti-venom capacity of crude bark extracts of Alstonia parvifolia Merr.Methods:Gas chromatography-mass spectrometry(GC-MS)and energy dispersive X-ray analyses were accom...Objective:To evaluate antioxidant,cytotoxic,and anti-venom capacity of crude bark extracts of Alstonia parvifolia Merr.Methods:Gas chromatography-mass spectrometry(GC-MS)and energy dispersive X-ray analyses were accomplished to characterize the chemical constituents of Alstonia parvifolia.Biochemical characterization was evaluated using an inhibitory phospholipase A_(2)(PLA_(2))assay,DPPH,and cytotoxicity assays.Using the constituents listed in the GC-MS analyses,molecular docking was conducted to inspect the binding energies between the chosen compounds and selected PLA_(2) isoforms.Results:GC-MS analyses showed that the Alstonia parvifolia crude extract consisted predominantly of acetylmarinobufogenin(14.89%),γ-sitosterol(10.44%),3-O-methyl-D-glucose(5.88%),3,5-dimethoxy-4-hydroxyphenylacetic acid(5.30%),(2α,5α)-17-methoxyaspidofractinin-3-one(AFM)(4.08%),and 2,3,5,6,7,8,9-heptahydro-1-phenyl-5-(p-chlorophenylimino)-1H-benzo[e][1,4]thiazepine(HPT)(1.37%).The principal elemental components of Alstonia parvifolia were Ca(4.012%)and K(1.496%),as exhibited by energy dispersive X-ray examination.Alstonia parvifolia showed significant free radical scavenging ability(IC_(50):0.287 mg/mL)and was non-cytotoxic to normal HDFn cells(IC_(50)>100μg/mL).Moreover,Alstonia parvifolia was favorably cytotoxic to MCF-7(IC_(50):4.42µg/mL),followed by H69PR,HT-29,and THP-1,with IC_(50) values of 4.94,5.07,and 6.27µg/mL,respectively.Alstonia parvifolia also displayed notable inhibition against PLA_(2) activity of Naja philippinensis Taylor venom with IC_(50) of(15.2±1.8)μg/mL.Docking and cluster analyses projected negative binding energies from AFM(−6.36 to−9.68 kcal/mol),HPT(−7.38 to−9.77 kcal/mol),and acetylmarinobufogenin(−7.22 to−9.59 kcal/mol).These calculations were for the particular interactions of Alstonia parvifolia constituents to PLA_(2) homologues where the utmost affinity was detected in HPT owing to the dipole interactions with amino acid residues.Conclusions:The bark extract of Alstonia parvifolia shows great potential as an anti-venom agent due to its low cytotoxic profile,remarkable PLA_(2) inhibition,and docking binding energies between its bioactive constituents and PLA_(2) homologues.展开更多
Competition at metal-ligand interfaces has long been recognized as a pivotal process in constructing multiscale nanostructures,wherein there is a delicate balance of coordination that yields novel structures and prope...Competition at metal-ligand interfaces has long been recognized as a pivotal process in constructing multiscale nanostructures,wherein there is a delicate balance of coordination that yields novel structures and properties.However,elucidating the underlying mechanisms and establishing design principles remain challenging.Here,we strategically introduce competition between Au(I)-thiolates and Na2S(inorganic sulfur),leading to the synthesis of an organic-inorganic hybrid-sulfur-containing cluster[Au_(22)(SR)_(17)S_(3)]−(SR=thiolate).X-ray absorption spectroscopy and tandem mass spectrometry revealed its structural features,where the gold sulfido cores(inorganic sulfide)were protected by gold-thiolates(organosulfur).More importantly,real-time electrospray ionization-mass spectrometry elucidated its formation process as a three-stage mechanism involving seed incubation,associative growth,and size-focusing.Through the extension of this approach,the nuclearity of gold sulfido clusters became tunable(e.g.,[Au_(22)(SR)_(14)S_(5)]^(2−)).This work not only introduces a novel category of atomically precise gold nanomaterials with appealing highlyemissive properties but also provides insights into the design of coordination competition for precise composition engineering of metal nanomaterials.展开更多
Background Recently, due to the rapid development of proteomic techniques, great advance has been made in many scientific fields. We aimed to use magnetic beads （liquid chip） based matrix-assisted laser desorption/i...Background Recently, due to the rapid development of proteomic techniques, great advance has been made in many scientific fields. We aimed to use magnetic beads （liquid chip） based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS） technology to screen distinctive biomarkers for lung adenocarcinoma （adCA）, and to establish the diagnostic protein profiles. Methods Using weak cation exchange magnetic beads （MB-WCX） to isolate and purify low molecular weight proteins from sera of 35 lung adCA, 46 benign lung diseases （BLDs） and 44 healthy individuals. The resulting spectra gained by anchor chip-MALDI-TOF-MS were analyzed by ClinProTools and a pattern recognition genetic algorithm （GA）. Results In the working mass range of 800-10 000 Da, 99 distinctive peaks were resolved in lung adCA versus BLDs, while 101 peaks were resolved in lung adCA versus healthy persons. The profile gained by GA that could distinguish adCA from BLDs was comprised of 4053.88, 4209.57 and 3883.33 Da with sensitivity of 80%, specificity of 93%, while that could separate adCA from healthy control was comprised of 2951.83 Da and 4209.73 Da with sensitivity of 94%, specificity of 95%. The sensitivity provided by carcinoembryonic antigen （CEA） in this experiment was significantly lower than our discriminatory profiles （P 〈0.005）. We further identified a eukaryotic peptide chain release factor GTP-binding subunit （eRF3b） （4209 Da） and a complement C3f （1865 Da） that may serve as candidate biomarkers for lung adCA. Conclusion Magnetic beads based MALDI-TOF-MS technology can rapidly and effectively screen distinctive proteins/polypeptides from sera of lung adCA patients and controls, which has potential value for establishing a new diagnostic method for lung adCA.展开更多
Background: Exercise, as the cornerstone of pulmonary rehabilitation, is recommended to chronic obstructive pulmonary disease (COPD) patients. The underlying molecular basis and metabolic process were not fully elucid...Background: Exercise, as the cornerstone of pulmonary rehabilitation, is recommended to chronic obstructive pulmonary disease (COPD) patients. The underlying molecular basis and metabolic process were not fully elucidated. Methods: Sprague-Dawley rats were classified into five groups: non-COPD/rest ( n = 8), non-COPD/exercise ( n = 7), COPD/rest ( n = 7), COPD/medium exercise ( n = 10), and COPD/intensive exercise ( n = 10). COPD animals were exposed to cigarette smoke and lipopolysaccharide instillation for 90 days, while the non-COPD control animals were exposed to room air. Non-COPD/exercise and COPD/medium exercise animals were trained on a treadmill at a decline of 5° and a speed of 15 m/min while animals in the COPD/intensive exercise group were trained at a decline of 5° and a speed of 18 m/min. After eight weeks of exercise/rest, we used ultrasonography, immunohistochemistry, transmission electron microscopy, oxidative capacity of mitochondria, airflow-assisted desorption electrospray ionization-mass spectrometry imaging (AFADESI-MSI), and transcriptomics analyses to assess rectal femoris (RF). Results: At the end of 90 days, COPD rats’ weight gain was smaller than control by 59.48 ± 15.33 g ( P = 0.0005). The oxidative muscle fibers proportion was lower ( P < 0.0001). At the end of additional eight weeks of exercise/rest, compared to COPD/rest, COPD/medium exercise group showed advantages in weight gain, femoral artery peak flow velocity (Δ58.22 mm/s, 95% CI: 13.85-102.60 mm/s, P = 0.0104), RF diameters (Δ0.16 mm, 95% CI: 0.04-0.28 mm, P = 0.0093), myofibrils diameter (Δ0.06 μm, 95% CI: 0.02-0.10 μm, P = 0.006), oxidative muscle fiber percentage (Δ4.84%, 95% CI: 0.15-9.53%, P = 0.0434), mitochondria oxidative phosphorylate capacity ( P < 0.0001). Biomolecules spatial distribution in situ and bioinformatic analyses of transcriptomics suggested COPD-related alteration in metabolites and gene expression, which can be impacted by exercise. Conclusion: COPD rat model had multi-level structure and function impairment, which can be mitigated by exercise.展开更多
文摘This paper provides a systematic review of Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging(MALDI-MSI),encompassing its technical principles,experimental workflows,matrix optimization strategies,and recent advancements in plant science applications.It highlights the method's groundbreaking applications in spatial mapping of plant metabolites,dynamic hormone monitoring,and functional studies of tissue microdomains,while offering critical insights into current technical limitations and future research directions.
文摘In this paper, a sensitive and specific liquid chromatography-electrospray ionisation-mass spectrometry （LC-ESI-MS） method has been developed and validated for the identification and determination of seven flavonoids, namely epimedin A, epimedin B, epimedin C, icariin, sagittatoside B, 2＂-O-rhamnosyl icariside II, and baohuoside I in Epimedium from different sources.
基金supported by the National Key R&D Pro-gram of China (No. 2018YFD0901102)the Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams (No. 2020KJ151)+1 种基金the Special Scientific Research Funds for Central Non-profit Institutes,Chinese Academy of Fishery Sciences (No. 2020 TD69)the China Agriculture Research System (No. CARS-50)。
文摘In this study,protein from Porphyra haitanensis was used as raw material to prepare an antioxidant peptide,and its antioxidant activity was evaluated in vitro.A model of H_2O_2-induced oxidative damage in Hep G2 cells was established,and the effects of Porphyra haitanensis hydrolysates (PHHs) on superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were detected.Finally,the structure of PHHs was identified by ESI-MS/MS.The results showed that the 1,1-diphenyl-2-pyridylhydrazine(DPPH)-free radical-scavenging ability of PHHs was the strongest (59.28%at 1.0 mg m L~(-1)) when hydrolyzed with an acidic protease for 4 h.PHHs with different concentrations had protective effects on H_2O_2-induced damage to Hep G2 cells,and the protective effect was enhanced with increasing concentrations.When the level was 400μg m L~(-1),the cell survival rate was as high as 88.62%.Moreover,PHHs can significantly reduce oxidative damage to Hep G2 cells by H_2O_2,improve SOD activity,and reduce MDA content.The tetrapeptide Asp-Lys-Ser-Thr,with a molecular weight of 448 Da,was identified as an important fraction of PHHs by high-resolution mass spectrometry.
基金the University Grants Com‐mission(UGC-BSR Research Start-up-Grant:F30-409/2018),India.
文摘This study focused,for the first time,on the effect of ultrasonic features on the extraction efficiency of secondary metabolites in mustard seed cake(MSC).The nematostatic potential of sonicated seed cake was examined against the second-stage juveniles(J2s)of root-knot nematode,Meloidogyne javanica.The results show that a 35 ppm(parts per million)concentration of a sonicated extract(SE)sample of MSC caused 65%J2s mortality at 18 h exposure period in vitro.It also significantly suppressed the root-knot index(RKI=0.94)in tomato roots.The lethal concentration values for SE were 51.76,29.79,and 13.34 ppm,respectively,at 6,12,and 18 h of the exposure period,and the lethal concentration values for the non-sonicated extract(NSE)sample were 116.95,76.38,and 55.59 ppm,respectively,at similar exposure time.Sinapine and gluconapin were identified as the major compounds in ultrasonic-assisted MSC.Because of the high extraction efficiency of metabolites in the SE,all treat‐ments of SE were shown to be antagonistic to J2s.Thus,this study of ultrasonication activity-based profiling of MSC may help generate target-based compounds at a scale relevant to the control of disease caused by nematodes in economic crops.
基金supported by the De La Salle University Science Foundation in coordination with the University Research Coordination Office(Project number:18FU2TAY16-3TAY17).
文摘Objective:To evaluate antioxidant,cytotoxic,and anti-venom capacity of crude bark extracts of Alstonia parvifolia Merr.Methods:Gas chromatography-mass spectrometry(GC-MS)and energy dispersive X-ray analyses were accomplished to characterize the chemical constituents of Alstonia parvifolia.Biochemical characterization was evaluated using an inhibitory phospholipase A_(2)(PLA_(2))assay,DPPH,and cytotoxicity assays.Using the constituents listed in the GC-MS analyses,molecular docking was conducted to inspect the binding energies between the chosen compounds and selected PLA_(2) isoforms.Results:GC-MS analyses showed that the Alstonia parvifolia crude extract consisted predominantly of acetylmarinobufogenin(14.89%),γ-sitosterol(10.44%),3-O-methyl-D-glucose(5.88%),3,5-dimethoxy-4-hydroxyphenylacetic acid(5.30%),(2α,5α)-17-methoxyaspidofractinin-3-one(AFM)(4.08%),and 2,3,5,6,7,8,9-heptahydro-1-phenyl-5-(p-chlorophenylimino)-1H-benzo[e][1,4]thiazepine(HPT)(1.37%).The principal elemental components of Alstonia parvifolia were Ca(4.012%)and K(1.496%),as exhibited by energy dispersive X-ray examination.Alstonia parvifolia showed significant free radical scavenging ability(IC_(50):0.287 mg/mL)and was non-cytotoxic to normal HDFn cells(IC_(50)>100μg/mL).Moreover,Alstonia parvifolia was favorably cytotoxic to MCF-7(IC_(50):4.42µg/mL),followed by H69PR,HT-29,and THP-1,with IC_(50) values of 4.94,5.07,and 6.27µg/mL,respectively.Alstonia parvifolia also displayed notable inhibition against PLA_(2) activity of Naja philippinensis Taylor venom with IC_(50) of(15.2±1.8)μg/mL.Docking and cluster analyses projected negative binding energies from AFM(−6.36 to−9.68 kcal/mol),HPT(−7.38 to−9.77 kcal/mol),and acetylmarinobufogenin(−7.22 to−9.59 kcal/mol).These calculations were for the particular interactions of Alstonia parvifolia constituents to PLA_(2) homologues where the utmost affinity was detected in HPT owing to the dipole interactions with amino acid residues.Conclusions:The bark extract of Alstonia parvifolia shows great potential as an anti-venom agent due to its low cytotoxic profile,remarkable PLA_(2) inhibition,and docking binding energies between its bioactive constituents and PLA_(2) homologues.
基金supported by the National Natural Science Foundation of China(grant no.22071174)support from the Ministry of Education,Singapore,Academic Research Fund(AcRF)grant no.A-8000054-01-00.T.Chen acknowledges the University Development Fund,Research Start-up Fund(grant no.UDF01002910)+1 种基金support from the Natural Sciences and Engineering Research Council(NSERC),CanadaThis research used resources of the Advanced Photon Source,an Office of Science User Facility operated for the US Department of Energy(DOE)Office of Science by Argonne National Laboratory and was supported by the US DOE under contract no.DE-AC02-06CH11357 and the Canadian Light Source(CLS)and its funding partners.
文摘Competition at metal-ligand interfaces has long been recognized as a pivotal process in constructing multiscale nanostructures,wherein there is a delicate balance of coordination that yields novel structures and properties.However,elucidating the underlying mechanisms and establishing design principles remain challenging.Here,we strategically introduce competition between Au(I)-thiolates and Na2S(inorganic sulfur),leading to the synthesis of an organic-inorganic hybrid-sulfur-containing cluster[Au_(22)(SR)_(17)S_(3)]−(SR=thiolate).X-ray absorption spectroscopy and tandem mass spectrometry revealed its structural features,where the gold sulfido cores(inorganic sulfide)were protected by gold-thiolates(organosulfur).More importantly,real-time electrospray ionization-mass spectrometry elucidated its formation process as a three-stage mechanism involving seed incubation,associative growth,and size-focusing.Through the extension of this approach,the nuclearity of gold sulfido clusters became tunable(e.g.,[Au_(22)(SR)_(14)S_(5)]^(2−)).This work not only introduces a novel category of atomically precise gold nanomaterials with appealing highlyemissive properties but also provides insights into the design of coordination competition for precise composition engineering of metal nanomaterials.
基金This work was supported by grants from the National Natural Science Foundation of China （No. 30570795） and Program for New Century Excellent Talents in University （No. NECT-06-0845） and the Program in Science and Technology of Xi＇an, Shaanxi Province （No. S F08009（1））.Acknowledgement： We are grateful to HU Xiao-hui for the technical guidance.
文摘Background Recently, due to the rapid development of proteomic techniques, great advance has been made in many scientific fields. We aimed to use magnetic beads （liquid chip） based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS） technology to screen distinctive biomarkers for lung adenocarcinoma （adCA）, and to establish the diagnostic protein profiles. Methods Using weak cation exchange magnetic beads （MB-WCX） to isolate and purify low molecular weight proteins from sera of 35 lung adCA, 46 benign lung diseases （BLDs） and 44 healthy individuals. The resulting spectra gained by anchor chip-MALDI-TOF-MS were analyzed by ClinProTools and a pattern recognition genetic algorithm （GA）. Results In the working mass range of 800-10 000 Da, 99 distinctive peaks were resolved in lung adCA versus BLDs, while 101 peaks were resolved in lung adCA versus healthy persons. The profile gained by GA that could distinguish adCA from BLDs was comprised of 4053.88, 4209.57 and 3883.33 Da with sensitivity of 80%, specificity of 93%, while that could separate adCA from healthy control was comprised of 2951.83 Da and 4209.73 Da with sensitivity of 94%, specificity of 95%. The sensitivity provided by carcinoembryonic antigen （CEA） in this experiment was significantly lower than our discriminatory profiles （P 〈0.005）. We further identified a eukaryotic peptide chain release factor GTP-binding subunit （eRF3b） （4209 Da） and a complement C3f （1865 Da） that may serve as candidate biomarkers for lung adCA. Conclusion Magnetic beads based MALDI-TOF-MS technology can rapidly and effectively screen distinctive proteins/polypeptides from sera of lung adCA patients and controls, which has potential value for establishing a new diagnostic method for lung adCA.
基金supported by grants from Chinese Academy of Medical Sciences,Innovation Fund for Medical Sciences(CIFMS)(No.2021-I2M-1-049)and(2)China-Japan Friendship Hospital Foundation for Young Scholars(No.2018-1-QN-11).
文摘Background: Exercise, as the cornerstone of pulmonary rehabilitation, is recommended to chronic obstructive pulmonary disease (COPD) patients. The underlying molecular basis and metabolic process were not fully elucidated. Methods: Sprague-Dawley rats were classified into five groups: non-COPD/rest ( n = 8), non-COPD/exercise ( n = 7), COPD/rest ( n = 7), COPD/medium exercise ( n = 10), and COPD/intensive exercise ( n = 10). COPD animals were exposed to cigarette smoke and lipopolysaccharide instillation for 90 days, while the non-COPD control animals were exposed to room air. Non-COPD/exercise and COPD/medium exercise animals were trained on a treadmill at a decline of 5° and a speed of 15 m/min while animals in the COPD/intensive exercise group were trained at a decline of 5° and a speed of 18 m/min. After eight weeks of exercise/rest, we used ultrasonography, immunohistochemistry, transmission electron microscopy, oxidative capacity of mitochondria, airflow-assisted desorption electrospray ionization-mass spectrometry imaging (AFADESI-MSI), and transcriptomics analyses to assess rectal femoris (RF). Results: At the end of 90 days, COPD rats’ weight gain was smaller than control by 59.48 ± 15.33 g ( P = 0.0005). The oxidative muscle fibers proportion was lower ( P < 0.0001). At the end of additional eight weeks of exercise/rest, compared to COPD/rest, COPD/medium exercise group showed advantages in weight gain, femoral artery peak flow velocity (Δ58.22 mm/s, 95% CI: 13.85-102.60 mm/s, P = 0.0104), RF diameters (Δ0.16 mm, 95% CI: 0.04-0.28 mm, P = 0.0093), myofibrils diameter (Δ0.06 μm, 95% CI: 0.02-0.10 μm, P = 0.006), oxidative muscle fiber percentage (Δ4.84%, 95% CI: 0.15-9.53%, P = 0.0434), mitochondria oxidative phosphorylate capacity ( P < 0.0001). Biomolecules spatial distribution in situ and bioinformatic analyses of transcriptomics suggested COPD-related alteration in metabolites and gene expression, which can be impacted by exercise. Conclusion: COPD rat model had multi-level structure and function impairment, which can be mitigated by exercise.
