Objective: To examine the expression of the tau protein and mi-crotubule-associated proteins (MAP) in the testicular interstitium of aged and young rats. Methods: Sprague-Dawley male rats were divided into a young gro...Objective: To examine the expression of the tau protein and mi-crotubule-associated proteins (MAP) in the testicular interstitium of aged and young rats. Methods: Sprague-Dawley male rats were divided into a young group (6 months) and an aged group (28 months) of 10 animals each. The two-step immunohistochemistry method with the antibody against tau protein and MAPa was performed with the testis tissues. Results: The immunoreactive cells of the testicular interstilial tau protein were significantly increased (P<0.01) and those of the MAP significantly decreased (P<0.01) in the aged than in the young rats. Conclusion: The changes in the expression of the tau protein and MAP may be related to the aging process of the testis.展开更多
目的:探讨大黄酸对人肾小管上皮细胞(HK-2)血小板反应蛋白1(TSP-1)和转化生长因子β1(TGF-β1)表达的影响。方法:体外培养HK-2细胞,观察AngⅡ(1×10-7mol/L)诱导下低、中和高浓度的大黄酸(10μg/ml、20μg/ml和40μg/ml)对HK-2TSP-1...目的:探讨大黄酸对人肾小管上皮细胞(HK-2)血小板反应蛋白1(TSP-1)和转化生长因子β1(TGF-β1)表达的影响。方法:体外培养HK-2细胞,观察AngⅡ(1×10-7mol/L)诱导下低、中和高浓度的大黄酸(10μg/ml、20μg/ml和40μg/ml)对HK-2TSP-1、TGF-β1的mRNA和蛋白表达的影响。采用Real Time PCR检测细胞TSP-1、TGF-β1mRNA表达,采用Westernb lot检测TSP-1、TGF-β1蛋白表达。培养上清液中TGF-β1和活性TGF-β1检测采用ELISA法。结果:与空白对照组比较,AngⅡ(1×10-7mol/L)明显上调了HK-2TSP-1的mRNA表达(3.84±0.48vs1.32±0.19;P<0.05)、伴有TGF-β1的mRNA表达增加(3.68±0.46vs1.18±0.13;P<0.05)。同时AngⅡ(1×10-7mol/L)也上调TSP-1蛋白表达(0.5246±0.0936 vs 0.1015±0.0768;P<0.01),伴有TGF-β1蛋白表达的增加(0.5948±0.0624 vs 0.1264±0.0811;P<0.01)。与AngⅡ阳性对照组比较,中浓度和高浓度大黄酸明显抑制了AngⅡ(1×10-7mol/L)诱导的TSP-1、TGF-β1的mRNA(P<0.05,P<0.01)和蛋白表达(P<0.05,P<0.01)。中浓度大黄酸干预组总的TGF-β1含量和活性TGF-β1含量均减少,(P<0.05);高浓度大黄酸干预组总的TGF-β1含量和活性TGF-β1含量均明显减少(P<0.01)。结论:大黄酸能抑制人肾小管上皮细胞TSP-1、TGF-β1的mRNA和蛋白表达,抑制TGF-β分泌。展开更多
目的对比研究心肌间质脂肪浸润和致心律失常性右心室心肌病(arrhythmogenic right ventricular cardiomyopathy,ARVC)的病理学特点,探讨其发生发展的相关因素及影响。方法收集105例心肌间质有脂肪无纤维组织浸润病变和6例ARVC的尸检资料...目的对比研究心肌间质脂肪浸润和致心律失常性右心室心肌病(arrhythmogenic right ventricular cardiomyopathy,ARVC)的病理学特点,探讨其发生发展的相关因素及影响。方法收集105例心肌间质有脂肪无纤维组织浸润病变和6例ARVC的尸检资料,对比二者的病变特点,分析心肌间质脂肪浸润程度与年龄、冠状动脉狭窄程度的相互关系。结果 (1)105例病变表现为心肌间质仅有脂肪组织浸润和渐进的心肌萎缩;6例ARVC特征性的病变是右心室为主的心肌层被广泛的纤维脂肪组织所替代,伴有心肌萎缩或炎细胞浸润。(2)心肌间质脂肪浸润与年龄增长和冠状动脉狭窄程度无关(P>0.05)。(3)心肌间质脂肪浸润伴心肌细胞萎缩率为57.14%(60/105),轻度组(7/42,16.67%)与中度组(39/47,82.98%)和重度组(14/16,87.50%)比较差异有统计学意义(P<0.01);中度组与重度组比较差异无统计学意义(P>0.05)。(4)14例中重度心肌间质脂肪浸润和5例ARVC均表现为心源性猝死。结论人体心肌间质脂肪浸润常常伴有心肌细胞萎缩,其发生发展与年龄增长和冠脉狭窄无关;中重度心肌间质脂肪浸润和ARVC可发生心源性猝死。展开更多
文摘Objective: To examine the expression of the tau protein and mi-crotubule-associated proteins (MAP) in the testicular interstitium of aged and young rats. Methods: Sprague-Dawley male rats were divided into a young group (6 months) and an aged group (28 months) of 10 animals each. The two-step immunohistochemistry method with the antibody against tau protein and MAPa was performed with the testis tissues. Results: The immunoreactive cells of the testicular interstilial tau protein were significantly increased (P<0.01) and those of the MAP significantly decreased (P<0.01) in the aged than in the young rats. Conclusion: The changes in the expression of the tau protein and MAP may be related to the aging process of the testis.
文摘目的:探讨大黄酸对人肾小管上皮细胞(HK-2)血小板反应蛋白1(TSP-1)和转化生长因子β1(TGF-β1)表达的影响。方法:体外培养HK-2细胞,观察AngⅡ(1×10-7mol/L)诱导下低、中和高浓度的大黄酸(10μg/ml、20μg/ml和40μg/ml)对HK-2TSP-1、TGF-β1的mRNA和蛋白表达的影响。采用Real Time PCR检测细胞TSP-1、TGF-β1mRNA表达,采用Westernb lot检测TSP-1、TGF-β1蛋白表达。培养上清液中TGF-β1和活性TGF-β1检测采用ELISA法。结果:与空白对照组比较,AngⅡ(1×10-7mol/L)明显上调了HK-2TSP-1的mRNA表达(3.84±0.48vs1.32±0.19;P<0.05)、伴有TGF-β1的mRNA表达增加(3.68±0.46vs1.18±0.13;P<0.05)。同时AngⅡ(1×10-7mol/L)也上调TSP-1蛋白表达(0.5246±0.0936 vs 0.1015±0.0768;P<0.01),伴有TGF-β1蛋白表达的增加(0.5948±0.0624 vs 0.1264±0.0811;P<0.01)。与AngⅡ阳性对照组比较,中浓度和高浓度大黄酸明显抑制了AngⅡ(1×10-7mol/L)诱导的TSP-1、TGF-β1的mRNA(P<0.05,P<0.01)和蛋白表达(P<0.05,P<0.01)。中浓度大黄酸干预组总的TGF-β1含量和活性TGF-β1含量均减少,(P<0.05);高浓度大黄酸干预组总的TGF-β1含量和活性TGF-β1含量均明显减少(P<0.01)。结论:大黄酸能抑制人肾小管上皮细胞TSP-1、TGF-β1的mRNA和蛋白表达,抑制TGF-β分泌。
文摘目的对比研究心肌间质脂肪浸润和致心律失常性右心室心肌病(arrhythmogenic right ventricular cardiomyopathy,ARVC)的病理学特点,探讨其发生发展的相关因素及影响。方法收集105例心肌间质有脂肪无纤维组织浸润病变和6例ARVC的尸检资料,对比二者的病变特点,分析心肌间质脂肪浸润程度与年龄、冠状动脉狭窄程度的相互关系。结果 (1)105例病变表现为心肌间质仅有脂肪组织浸润和渐进的心肌萎缩;6例ARVC特征性的病变是右心室为主的心肌层被广泛的纤维脂肪组织所替代,伴有心肌萎缩或炎细胞浸润。(2)心肌间质脂肪浸润与年龄增长和冠状动脉狭窄程度无关(P>0.05)。(3)心肌间质脂肪浸润伴心肌细胞萎缩率为57.14%(60/105),轻度组(7/42,16.67%)与中度组(39/47,82.98%)和重度组(14/16,87.50%)比较差异有统计学意义(P<0.01);中度组与重度组比较差异无统计学意义(P>0.05)。(4)14例中重度心肌间质脂肪浸润和5例ARVC均表现为心源性猝死。结论人体心肌间质脂肪浸润常常伴有心肌细胞萎缩,其发生发展与年龄增长和冠脉狭窄无关;中重度心肌间质脂肪浸润和ARVC可发生心源性猝死。
