Dynamical decoupling(DD)is normally ineffective when applied to DC measurement.In its straightforward implementation,DD nulls out DC signal as well while suppressing noise.This work proposes a phase relay method that ...Dynamical decoupling(DD)is normally ineffective when applied to DC measurement.In its straightforward implementation,DD nulls out DC signal as well while suppressing noise.This work proposes a phase relay method that is capable of continuously interrogating the DC signal over many DD cycles.We illustrate its efficacy when applied to the measurement of a weak DC magnetic field with an atomic spinor Bose-Einstein condensate.Sensitivities approaching standard quantum limit or Heisenberg limit are potentially realizable for a coherent spin state or a squeezed spin state of 10000 atoms,respectively,while ambient laboratory level noise is suppressed by DD.Our work offers a practical approach to mitigate the limitations of DD to DC measurement and would find other applications for resorting coherence in quantum sensing and quantum information processing research.展开更多
The performance of space cold atom clocks（SCACs） should be improved thanks to the microgravity environment in space.The microwave interrogation cavity is a key element in a SCAC.In this paper,we develop a microwave ...The performance of space cold atom clocks（SCACs） should be improved thanks to the microgravity environment in space.The microwave interrogation cavity is a key element in a SCAC.In this paper,we develop a microwave interrogation cavity especially for the rubidium SCAC.The interrogation cavity has two microwave interaction zones with a single feedin source,which is located at the center of the cavity for symmetric coupling excitation and to ensure that the two interaction zones are in phase.The interrogation cavity has a measured resonance frequency of 6.835056471 GHz with a loaded quality factor of nearly 4200,which shows good agreement with simulation results.We measure the Rabi frequency of the clock transition of the rubidium atom in each microwave interaction zone,and subsequently demonstrate that the distributions of the magnetic field in the two interaction zones are the same and meet all requirements of the rubidium SCAC.展开更多
The smuggling of special nuclear materials(SNMs)across national borders is becoming a serious threat to nuclear nonproliferation.This paper presents a feasibility study on the rapid interrogation of concealed SNMs by ...The smuggling of special nuclear materials(SNMs)across national borders is becoming a serious threat to nuclear nonproliferation.This paper presents a feasibility study on the rapid interrogation of concealed SNMs by combining scattering and transmission nuclear resonance fluorescence(s NRF and t NRF)spectroscopy.In s NRF spectroscopy,SNMs such as^(235,238)U are excited by a wide-band photon beam of appropriate energy and exhibit unique NRF signatures.Monte Carlo simulations show that one-dimensional scans can realize isotopic identification of concealed^(235,238)U when the detector array used for interrogation has sufficiently high energy resolution.The simulated isotopic ratio^(235U/238)U is in good agreement with the theoretical value when the SNMs are enclosed in relatively thin iron.This interrogation is followed by t NRF spectroscopy using a narrow-band photon beam with the goal of obtaining tomographic images of the concealed SNMs.The reconstructed image clearly reveals the position of the isotope^(235)U inside an iron rod.It is shown that the interrogation time of s NRF and t NRF spectroscopy is one order of magnitude lower than that when only t NRF spectroscopy is used and results in a missed-detection rate of 10^(-3).The proposed method can also be applied for isotopic imaging of other SNMs such as^(239,240)Pu and^(237)Np.展开更多
In accordance with the characteristics of wavelength shift detection in fiber grating sensor interrogation system,the wavelength interrogation system which uses linear InGaAs as the spectrum receiver is proposed.Orien...In accordance with the characteristics of wavelength shift detection in fiber grating sensor interrogation system,the wavelength interrogation system which uses linear InGaAs as the spectrum receiver is proposed.Orientation of optic spectrum line affects the silt of volume phase grating and size of InGaAs photosensitive unit,thus the calibration method is needed.Based on an analysis of InGaAs imaging model,least square curve fitting method is proposed to detect spectrum wavelength and InGaAs photosensitive unit position.The experimental results show that the methods are effective and the demodulation system precision is improved.展开更多
High-performance interrogation systems for optical fiber sensors are extensively required for environmental condition monitoring applications.In this article,we propose and demonstrate a Michelson interferometer(MI)in...High-performance interrogation systems for optical fiber sensors are extensively required for environmental condition monitoring applications.In this article,we propose and demonstrate a Michelson interferometer(MI)interrogation system based on an optoelectronic oscillator(OEO).The frequency of the OEO is related to the free spectral range(FSR)of the MI.Thus,when the FSR of the MI varies with a change in external physical factors,the frequency of the OEO shifts and can be used for interrogation.We demonstrate that the temperature sensitivity and interrogation resolution are 35.35 MHz/℃and 0.012℃,respectively.Such an OEO-based scheme enables wavelength-to-frequency mapping and promises a wide linear interrogation range,high resolution and high-speed interrogation.展开更多
This paper (constituting Part B) addresses active interrogation for detecting Special Nuclear Materials (SN- Ms) and includes description of the transformational Tensioned Metastable Fluid Detector (TMFD) based method...This paper (constituting Part B) addresses active interrogation for detecting Special Nuclear Materials (SN- Ms) and includes description of the transformational Tensioned Metastable Fluid Detector (TMFD) based method for optimal monitoring. One of the greatest difficulties in detection of SNMs by active interrogation is the task of distinguishing between the probing particles and the secondary particles that indicate the presence of SNMs. The TMFD’s selective insensitivity and γ photon blindness features are advantageous for alleviating this problem. The working principle of the TMFD is discussed along with its applications for security. The experimental work to date involving detection of small quantities of uranium with conventional detectors is discussed along with results of fission neutron detection. Statistically significant detection was achieved within 5 minutes of counting to ascertain and measure conclusive evidence for the presence of a 55g sample of uranium containing 235U. Results of simulations of three active detection techniques utilizing a TMFD system are presented. The process for using the TMFD to discriminate active source particles using timing and energy are described. These simulations indicate that it should be possible to utilize the TMFD system for optimal neutron-based interrogation of SNMs.展开更多
Anti-PD-1/PD-L1 immune checkpoint blockade(ICB)therapy has revolutionized clinical cancer treatment,while abnormal PD-L1 or HLA-l expression in patients can significantly impact the therapeutic efficacy.Somatic mutati...Anti-PD-1/PD-L1 immune checkpoint blockade(ICB)therapy has revolutionized clinical cancer treatment,while abnormal PD-L1 or HLA-l expression in patients can significantly impact the therapeutic efficacy.Somatic mutations in cancer cells that modulate these critical regulators are closely associated with tumor progression and ICB response.However,a systematic interpretation of cancer immune-related mutations is stll lacking.Here,we harnessed the ABEmax system to establish a large-scale sgRNA library encompassing approximately 820,000 sgRNAs that target all feasible serine/threonine/tyrosine residues across the human genome,which systematically unveiled thousands of novel mutations that decrease or augment PD-L1 or HLA-I expression.Beyond residues associated with phosphorylation events,our screens also identified functional mutations that affect mRNA or protein stability,DNA binding capacity,protein-protein interactions,and enzymatic catalytic activity,leading to either gene inactivation or activation.Notably,we uncovered certain mutations that concurrently modulate PD-L1 and HLA-I expression,represented by the clinically relevant mutation SETD2_Y1666.We demonstrated that this mutation induces consistent phenotypic effects across multiple cancer cell lines and enhances the efficacy of immunotherapy in different tumor models.Our findings provide an unprecedented resource of functional residues that regulate cancer immunosurveillance,offering valuable guidance for clinical diagnosis,ICB therapy,and the development of innovative drugs for cancer treatment.展开更多
The localized surface plasmon resonance metasurface is a research hotspot in the sensing field since it can enhance the light-matter interaction in the nanoscale,but the wavelength sensitivity is far from comparable w...The localized surface plasmon resonance metasurface is a research hotspot in the sensing field since it can enhance the light-matter interaction in the nanoscale,but the wavelength sensitivity is far from comparable with that of prism-coupled surface plasmon polariton(SPP).Herein,we propose and demonstrate an ultrasensitive angular interrogation sensor based on the transverse electric mode surface lattice resonance(SLR)mechanism in an all-metal metasurface.In theory,we derive the sensitivity function in detail and emphasize the refraction effect at the air-solution interface,which influences the SLR position and improves the sensitivity performance greatly in the wide-angle.In the measurement,a broadband light source substitutes the single-wavelength laser generally used in traditional angular sensing,and the measured SLR wavelength of broadband illuminant at normal incidence is defined as the single wavelength,avoiding the sensitivity loss from the large angle.The experimental sensitivity can reach 4304.35°/RIU,promoting an order of magnitude compared to those of SPP-sensors.This research provides a novel theory as well as the corresponding crucial approach to achieving ultrasensitive angular sensing.展开更多
The illicit trafficking of special nuclear materials(SNMs)poses a grave threat to global security and necessitates the development of effective nuclear material identification methods.This study investigated a method ...The illicit trafficking of special nuclear materials(SNMs)poses a grave threat to global security and necessitates the development of effective nuclear material identification methods.This study investigated a method to isotopically identify the SNMs,including^(233,235,238)U,^(239-242)Pu,and^(232)Th,based on the detection of delayedγ-rays from photofission fragments.The delayedγ-ray spectra resulting from the photofission of SNMs irradiated by a 14 MeVγbeam with a total of 10~9 were simulated using Geant4.Three high-yield fission fragments,namely^(138)Cs,^(89)Rb,and^(94)Y,were selected as candidate fragments for SNM identification.The yield ratios of these three fragments were calculated,and the results from the different SNMs were compared.The yield ratio of^(138)Cs/^(89)Rb was used to identify most SNMs,including^(233,235,238)U,^(242)Pu,and^(232)Th,with a confidence level above 95%.To identify^(239-241)Pu with the same confidence,a higher total number of 10^(11)γbeams is required.However,although the^(94)Y/^(89)Rb ratio is suitable for elementally identifying SNMs,isotopic identification is difficult.In addition,the count rate of the delayedγabove 3 MeV can be used to rapidly detect the presence of nuclear materials.展开更多
This paper presents the development of a cost-effective precision fiber Bragg grating （FBG） interrogation system using long-wavelength vertical-cavity surface-emitting laser （VCSEL）. Tuning properties of a long-wa...This paper presents the development of a cost-effective precision fiber Bragg grating （FBG） interrogation system using long-wavelength vertical-cavity surface-emitting laser （VCSEL）. Tuning properties of a long-wavelength VCSEL have been studied experimentally. An approximately quadratic dependence of its wavelength on the injection current has been observed. The overall design and key operations of this system including intensity normalization, peak detection, and quadratic curve fitting are introduced in detail. The results show that the system achieves an accuracy of 1.2 pm with a tuning range of 3 nm and a tuning rate of 1 kHz. It is demonstrated that this system is practical and effective by applied in the FBG transformer temperature monitoring.展开更多
Optical fiber optrodes are attractive sensing devices due to their ability to perform point measurement in remote locations. Mostly, they are oriented to biochemical sensing, quite often supported by fluorescent and s...Optical fiber optrodes are attractive sensing devices due to their ability to perform point measurement in remote locations. Mostly, they are oriented to biochemical sensing, quite often supported by fluorescent and spectroscopic techniques, but with the refractometric approach considered as well when the objective is of high measurement performance, particularly when the focus is on enhancing the measurand resolution. In this work, we address this subject, proposing and analyzing the characteristics of a fiber optic optrode relying on plasmonic interaction. A linearly tapered optical fiber tip is covered by a double overlay： the inner one - a silver thin film and over it - a dielectric layer, with this combination allowing to achieve, at a specific wavelength range, surface plasmonic resonance （SPR） interaction sensitive Typically, the interrogation of the SPR sensing to the refractive index of the surrounding medium. structures is performed, considering spectroscopic techniques, but in principle, a far better performance can be obtained, considering the reading of the phase of the light at a specific wavelength located within the spectral plasmonic resonance. This is the approach which is studied here in the context of the proposed optical fiber optrode configuration. The analysis performed shows the combination of a silver inner layer with a dielectric titanium oxide layer with tuned thicknesses enables sensitive phase reading and allows the operation of the fiber optic optrode sensor in the third telecommunication wavelength window.展开更多
We describe and experimentally demonstrate a measuring technique for Mach–Zehnder interferometer(MZI)based integrated photonic biochemical sensors. Our technique is based on the direct measurement of phase changes be...We describe and experimentally demonstrate a measuring technique for Mach–Zehnder interferometer(MZI)based integrated photonic biochemical sensors. Our technique is based on the direct measurement of phase changes between the arms of the MZI, achieved by signal modulation on one of the arms of the interferometer together with pseudoheterodyne detection, and it allows us to avoid the use of costly equipment such as tunable light sources or spectrum analyzers. The obtained output signal is intrinsically independent of wavelength, power variations, and global thermal variations, making it extremely robust and adequate for use in real conditions. Using a silicon-on-insulator MZI, we demonstrate the real-time monitoring of refractive index variations and achieve a detection limit of 4.1 × 10^(-6)refractive index units(RIU).展开更多
An interrogation system based on two semiconductor optical amplifiers for weak fiber Bragg gratings （FBGs） was proposed. The first semiconductor optical amplifier （SOA） was used to modulate the light, and the seco...An interrogation system based on two semiconductor optical amplifiers for weak fiber Bragg gratings （FBGs） was proposed. The first semiconductor optical amplifier （SOA） was used to modulate the light, and the second SOA separated the reflected signal from the different FBGs through delayed switching. The proposed system has lower insertion loss and higher spatial resolution, and can interrogate the time- and wavelength-division multiplexed FBG array. Up to 50 FBGs, with a reflectivity of 0.2% and a spatial resolution of 5 m along the optical fiber, were distinguished to demonstrate the interrogation system.展开更多
The development of two simple methods for wavelength-optical intensity modulation techniques for fiber Bragg grating （FBG） sensors is presented. The performance is evaluated by measuring the strain and temperature. ...The development of two simple methods for wavelength-optical intensity modulation techniques for fiber Bragg grating （FBG） sensors is presented. The performance is evaluated by measuring the strain and temperature. The first method consists of a narrow band source, an optical circulator, an FBG; and a power meter. The source and Bragg reflected signal from the FBG need to be matched to get linear results with good power levels. The source spectral power levels are very critical in this study. The power reflected from a matched reference FBG is fed into the measuring FBG in the second method. Since the FBGs are matched, the entire power is reflected back initially. During the measurement, the change in the measurand causes the reflected power from the sensing FBG to vary. A costly high resolution spectrum analyzer is required only during the characterization of the FBG and source. The performances of two interrogators are compared by measuring the strain and temperature. In the second method, the strain measurements can be made insensitive to the temperature variation by selecting a source with a flat spectrum at the measurement range. Highlights of these methods are the portability, cost effectiveness and better resolution.展开更多
Wireless interrogation of a rectangular microstrip patch antenna for strain measurement is investigated by simulations.To analyze the antenna performance,a microstrip line-feeding patch antenna at 10 GHz is designed.A...Wireless interrogation of a rectangular microstrip patch antenna for strain measurement is investigated by simulations.To analyze the antenna performance,a microstrip line-feeding patch antenna at 10 GHz is designed.A patch antenna wirelessly fed by a horn is proposed to measure the strain.The direction information of strain detected by the patch antenna is also considered.The strain can be detected both in the width and length directions.It is shown that the strain can be measured wirelessly using a standard horn antenna.This kind of wireless strain-sensing technique offers significant potential for wireless structural health monitoring(SHM),especially for high-end equipment.展开更多
A method to interrogate fiber Bragg grating vibration sensor by narrow line width light is demonstrated. The interrogation scheme takes advantage of the intensity modulation of narrow spectral bandwidth light, such as...A method to interrogate fiber Bragg grating vibration sensor by narrow line width light is demonstrated. The interrogation scheme takes advantage of the intensity modulation of narrow spectral bandwidth light, such as distributed feedback laser, when a reflection or transmission spectrum curve of an fiber Bragg grating （FBG） moves due to the strain which is applied on the sensor. The sensor＇s response to accelerating frequency and amplitude is measured by experiment. The factors which have impacts on the sensitivity of the interrogation system are also discussed.展开更多
This paper aims to study the embodiment and directive function of Cooperative Principle in trial process. By analyzing dialogues in the sample trial transcript, the author will find out the embodiment of Cooperative P...This paper aims to study the embodiment and directive function of Cooperative Principle in trial process. By analyzing dialogues in the sample trial transcript, the author will find out the embodiment of Cooperative Principle in trial language, and draw a conclusion that the Cooperative Principle is important to direct the judge to discriminate if the suspect is cooperative, which helps the interrogation succeed.展开更多
The advent of the digital age has pointed out the direction for the development of higher education.In this paper,the undergraduate course“Science of Interrogation”is taken as the research subject,the challenges of ...The advent of the digital age has pointed out the direction for the development of higher education.In this paper,the undergraduate course“Science of Interrogation”is taken as the research subject,the challenges of big data teaching reform in universities are analyzed systematically,and concrete measures,such as adhering to the concept of teaching reform under the condition of big data,establishing professional teaching teams,improving the curriculum construction level,emphasizing on being student-centered,and optimizing the reform of the curriculum evaluation system,are proposed to address these challenges.展开更多
Patients with tinnitus are associated with adverse psychological reactions to varying degrees.Psychological interventions are currently the primary treatment to help patients eliminate negative responses to tinnitus.T...Patients with tinnitus are associated with adverse psychological reactions to varying degrees.Psychological interventions are currently the primary treatment to help patients eliminate negative responses to tinnitus.Traditional Chinese medicine uses four diagnostic methods or consultations.Interrogation is one such method.Through consultation,doctors can analyze the causes of tinnitus in patients,collect an accurate description,and assess the severity of the condition.At the same time,when doctors ask about other symptoms in a guided manner,they help patients refocus their attention to the symptoms so that their perception of tinnitus is changed,the vicious circular thinking patterns are broken,and their quality of life improves.When the patient understands why the physician establishes a medical plan,they are willing to cooperate actively and participate in a constructive doctor-patient relationship.This article analyzes the clinical methods and techniques of diagnosing tinnitus patients from the vantage of psychosomatic medicine,which has particular significance for the clinical treatment of tinnitus.展开更多
Emily Dickinson is widely regarded as one of American's premier poets of the nineteenth century.In both style and content,her verse was,"revolutionary" in her day.Owing to the deep exploration of death a...Emily Dickinson is widely regarded as one of American's premier poets of the nineteenth century.In both style and content,her verse was,"revolutionary" in her day.Owing to the deep exploration of death and immortality,Emily Dickinson has become a puzzle in literary.As show in her poems,Dickinson's spiritual journey led her from na?ve nature-mysticism through disappointment,to a sacramental approach to immortality afterlife and further discouragement,culminating in a mature attitude of faithful unknowing and even more interrogating the immortality.This paper will base on the theory of the death and immortality through common people's fear to death,the essence of death,the significance of death to show the poetess's whole life-childhood,love,life condition,spiritual contradiction,and mainly her skeptical concept of death and immortality.展开更多
基金Project supported by the NSAF(Grant No.U1930201)the National Natural Science Foundation of China(Grant Nos.12274331,91836101,and 91836302)+1 种基金the National Key R&D Program of China(Grant No.2018YFA0306504)Innovation Program for Quantum Science and Technology(Grant No.2021ZD0302100).
文摘Dynamical decoupling(DD)is normally ineffective when applied to DC measurement.In its straightforward implementation,DD nulls out DC signal as well while suppressing noise.This work proposes a phase relay method that is capable of continuously interrogating the DC signal over many DD cycles.We illustrate its efficacy when applied to the measurement of a weak DC magnetic field with an atomic spinor Bose-Einstein condensate.Sensitivities approaching standard quantum limit or Heisenberg limit are potentially realizable for a coherent spin state or a squeezed spin state of 10000 atoms,respectively,while ambient laboratory level noise is suppressed by DD.Our work offers a practical approach to mitigate the limitations of DD to DC measurement and would find other applications for resorting coherence in quantum sensing and quantum information processing research.
基金Project supported by the National Natural Science Foundation of China(Grant No.11034008)the Fund from the Ministry of Science and Technology of China(Grant No.2013YQ09094304)the Youth Innovation Promotion Association,Chinese Academy of Sciences
文摘The performance of space cold atom clocks（SCACs） should be improved thanks to the microgravity environment in space.The microwave interrogation cavity is a key element in a SCAC.In this paper,we develop a microwave interrogation cavity especially for the rubidium SCAC.The interrogation cavity has two microwave interaction zones with a single feedin source,which is located at the center of the cavity for symmetric coupling excitation and to ensure that the two interaction zones are in phase.The interrogation cavity has a measured resonance frequency of 6.835056471 GHz with a loaded quality factor of nearly 4200,which shows good agreement with simulation results.We measure the Rabi frequency of the clock transition of the rubidium atom in each microwave interaction zone,and subsequently demonstrate that the distributions of the magnetic field in the two interaction zones are the same and meet all requirements of the rubidium SCAC.
基金supported by the National Natural Science Foundation of China(No.11675075)Youth Talent Project of Hunan Province,China(No.2018RS3096)+1 种基金Independent Research Project of Key Laboratory of Plasma Physics,CAEP(No.JCKYS2020212006)Innovation and Entrepreneurship Training Program for College Students of University of South China(No.X2019083)。
文摘The smuggling of special nuclear materials(SNMs)across national borders is becoming a serious threat to nuclear nonproliferation.This paper presents a feasibility study on the rapid interrogation of concealed SNMs by combining scattering and transmission nuclear resonance fluorescence(s NRF and t NRF)spectroscopy.In s NRF spectroscopy,SNMs such as^(235,238)U are excited by a wide-band photon beam of appropriate energy and exhibit unique NRF signatures.Monte Carlo simulations show that one-dimensional scans can realize isotopic identification of concealed^(235,238)U when the detector array used for interrogation has sufficiently high energy resolution.The simulated isotopic ratio^(235U/238)U is in good agreement with the theoretical value when the SNMs are enclosed in relatively thin iron.This interrogation is followed by t NRF spectroscopy using a narrow-band photon beam with the goal of obtaining tomographic images of the concealed SNMs.The reconstructed image clearly reveals the position of the isotope^(235)U inside an iron rod.It is shown that the interrogation time of s NRF and t NRF spectroscopy is one order of magnitude lower than that when only t NRF spectroscopy is used and results in a missed-detection rate of 10^(-3).The proposed method can also be applied for isotopic imaging of other SNMs such as^(239,240)Pu and^(237)Np.
文摘In accordance with the characteristics of wavelength shift detection in fiber grating sensor interrogation system,the wavelength interrogation system which uses linear InGaAs as the spectrum receiver is proposed.Orientation of optic spectrum line affects the silt of volume phase grating and size of InGaAs photosensitive unit,thus the calibration method is needed.Based on an analysis of InGaAs imaging model,least square curve fitting method is proposed to detect spectrum wavelength and InGaAs photosensitive unit position.The experimental results show that the methods are effective and the demodulation system precision is improved.
文摘High-performance interrogation systems for optical fiber sensors are extensively required for environmental condition monitoring applications.In this article,we propose and demonstrate a Michelson interferometer(MI)interrogation system based on an optoelectronic oscillator(OEO).The frequency of the OEO is related to the free spectral range(FSR)of the MI.Thus,when the FSR of the MI varies with a change in external physical factors,the frequency of the OEO shifts and can be used for interrogation.We demonstrate that the temperature sensitivity and interrogation resolution are 35.35 MHz/℃and 0.012℃,respectively.Such an OEO-based scheme enables wavelength-to-frequency mapping and promises a wide linear interrogation range,high resolution and high-speed interrogation.
文摘This paper (constituting Part B) addresses active interrogation for detecting Special Nuclear Materials (SN- Ms) and includes description of the transformational Tensioned Metastable Fluid Detector (TMFD) based method for optimal monitoring. One of the greatest difficulties in detection of SNMs by active interrogation is the task of distinguishing between the probing particles and the secondary particles that indicate the presence of SNMs. The TMFD’s selective insensitivity and γ photon blindness features are advantageous for alleviating this problem. The working principle of the TMFD is discussed along with its applications for security. The experimental work to date involving detection of small quantities of uranium with conventional detectors is discussed along with results of fission neutron detection. Statistically significant detection was achieved within 5 minutes of counting to ascertain and measure conclusive evidence for the presence of a 55g sample of uranium containing 235U. Results of simulations of three active detection techniques utilizing a TMFD system are presented. The process for using the TMFD to discriminate active source particles using timing and energy are described. These simulations indicate that it should be possible to utilize the TMFD system for optimal neutron-based interrogation of SNMs.
基金supported by funds from Changping Laboratory,the National Natural Science Foundation of China(NSFC 31930016)Beijing Advanced Innovation Center for Genomics at Peking University,and the Peking-Tsinghua Center for Life Sciences(to W.W.)+2 种基金Additional support was provided by the China Postdoctoral Science Foundation(2020M670031,to Y.L2020M670053,to Y-S.L.)the Taishan Scholarship(tsqn202312362,to Y-S.L.).
文摘Anti-PD-1/PD-L1 immune checkpoint blockade(ICB)therapy has revolutionized clinical cancer treatment,while abnormal PD-L1 or HLA-l expression in patients can significantly impact the therapeutic efficacy.Somatic mutations in cancer cells that modulate these critical regulators are closely associated with tumor progression and ICB response.However,a systematic interpretation of cancer immune-related mutations is stll lacking.Here,we harnessed the ABEmax system to establish a large-scale sgRNA library encompassing approximately 820,000 sgRNAs that target all feasible serine/threonine/tyrosine residues across the human genome,which systematically unveiled thousands of novel mutations that decrease or augment PD-L1 or HLA-I expression.Beyond residues associated with phosphorylation events,our screens also identified functional mutations that affect mRNA or protein stability,DNA binding capacity,protein-protein interactions,and enzymatic catalytic activity,leading to either gene inactivation or activation.Notably,we uncovered certain mutations that concurrently modulate PD-L1 and HLA-I expression,represented by the clinically relevant mutation SETD2_Y1666.We demonstrated that this mutation induces consistent phenotypic effects across multiple cancer cell lines and enhances the efficacy of immunotherapy in different tumor models.Our findings provide an unprecedented resource of functional residues that regulate cancer immunosurveillance,offering valuable guidance for clinical diagnosis,ICB therapy,and the development of innovative drugs for cancer treatment.
基金supported by the National Key R&D Program of China(Grant No.2021YFB3200100)the National Natural Science Foundation of China(Grant No.61931018 and 61974004)。
文摘The localized surface plasmon resonance metasurface is a research hotspot in the sensing field since it can enhance the light-matter interaction in the nanoscale,but the wavelength sensitivity is far from comparable with that of prism-coupled surface plasmon polariton(SPP).Herein,we propose and demonstrate an ultrasensitive angular interrogation sensor based on the transverse electric mode surface lattice resonance(SLR)mechanism in an all-metal metasurface.In theory,we derive the sensitivity function in detail and emphasize the refraction effect at the air-solution interface,which influences the SLR position and improves the sensitivity performance greatly in the wide-angle.In the measurement,a broadband light source substitutes the single-wavelength laser generally used in traditional angular sensing,and the measured SLR wavelength of broadband illuminant at normal incidence is defined as the single wavelength,avoiding the sensitivity loss from the large angle.The experimental sensitivity can reach 4304.35°/RIU,promoting an order of magnitude compared to those of SPP-sensors.This research provides a novel theory as well as the corresponding crucial approach to achieving ultrasensitive angular sensing.
基金supported by the National Key Research and Development Program(No.2022YFA1603300)the National Natural Science Foundation of China(Nos.U2230133,12305266,11921006,12405282)National Grand Instrument Project(No.2019YFF01014400)。
文摘The illicit trafficking of special nuclear materials(SNMs)poses a grave threat to global security and necessitates the development of effective nuclear material identification methods.This study investigated a method to isotopically identify the SNMs,including^(233,235,238)U,^(239-242)Pu,and^(232)Th,based on the detection of delayedγ-rays from photofission fragments.The delayedγ-ray spectra resulting from the photofission of SNMs irradiated by a 14 MeVγbeam with a total of 10~9 were simulated using Geant4.Three high-yield fission fragments,namely^(138)Cs,^(89)Rb,and^(94)Y,were selected as candidate fragments for SNM identification.The yield ratios of these three fragments were calculated,and the results from the different SNMs were compared.The yield ratio of^(138)Cs/^(89)Rb was used to identify most SNMs,including^(233,235,238)U,^(242)Pu,and^(232)Th,with a confidence level above 95%.To identify^(239-241)Pu with the same confidence,a higher total number of 10^(11)γbeams is required.However,although the^(94)Y/^(89)Rb ratio is suitable for elementally identifying SNMs,isotopic identification is difficult.In addition,the count rate of the delayedγabove 3 MeV can be used to rapidly detect the presence of nuclear materials.
文摘This paper presents the development of a cost-effective precision fiber Bragg grating （FBG） interrogation system using long-wavelength vertical-cavity surface-emitting laser （VCSEL）. Tuning properties of a long-wavelength VCSEL have been studied experimentally. An approximately quadratic dependence of its wavelength on the injection current has been observed. The overall design and key operations of this system including intensity normalization, peak detection, and quadratic curve fitting are introduced in detail. The results show that the system achieves an accuracy of 1.2 pm with a tuning range of 3 nm and a tuning rate of 1 kHz. It is demonstrated that this system is practical and effective by applied in the FBG transformer temperature monitoring.
文摘Optical fiber optrodes are attractive sensing devices due to their ability to perform point measurement in remote locations. Mostly, they are oriented to biochemical sensing, quite often supported by fluorescent and spectroscopic techniques, but with the refractometric approach considered as well when the objective is of high measurement performance, particularly when the focus is on enhancing the measurand resolution. In this work, we address this subject, proposing and analyzing the characteristics of a fiber optic optrode relying on plasmonic interaction. A linearly tapered optical fiber tip is covered by a double overlay： the inner one - a silver thin film and over it - a dielectric layer, with this combination allowing to achieve, at a specific wavelength range, surface plasmonic resonance （SPR） interaction sensitive Typically, the interrogation of the SPR sensing to the refractive index of the surrounding medium. structures is performed, considering spectroscopic techniques, but in principle, a far better performance can be obtained, considering the reading of the phase of the light at a specific wavelength located within the spectral plasmonic resonance. This is the approach which is studied here in the context of the proposed optical fiber optrode configuration. The analysis performed shows the combination of a silver inner layer with a dielectric titanium oxide layer with tuned thicknesses enables sensitive phase reading and allows the operation of the fiber optic optrode sensor in the third telecommunication wavelength window.
文摘We describe and experimentally demonstrate a measuring technique for Mach–Zehnder interferometer(MZI)based integrated photonic biochemical sensors. Our technique is based on the direct measurement of phase changes between the arms of the MZI, achieved by signal modulation on one of the arms of the interferometer together with pseudoheterodyne detection, and it allows us to avoid the use of costly equipment such as tunable light sources or spectrum analyzers. The obtained output signal is intrinsically independent of wavelength, power variations, and global thermal variations, making it extremely robust and adequate for use in real conditions. Using a silicon-on-insulator MZI, we demonstrate the real-time monitoring of refractive index variations and achieve a detection limit of 4.1 × 10^(-6)refractive index units(RIU).
基金This work was supported in part by the National Science Foundation of China, NSFC （Gram No. 61205070）, and the Major Program of the National Natural Science Foundation of China, NSFC （Grant No. 61290311）.
文摘An interrogation system based on two semiconductor optical amplifiers for weak fiber Bragg gratings （FBGs） was proposed. The first semiconductor optical amplifier （SOA） was used to modulate the light, and the second SOA separated the reflected signal from the different FBGs through delayed switching. The proposed system has lower insertion loss and higher spatial resolution, and can interrogate the time- and wavelength-division multiplexed FBG array. Up to 50 FBGs, with a reflectivity of 0.2% and a spatial resolution of 5 m along the optical fiber, were distinguished to demonstrate the interrogation system.
文摘The development of two simple methods for wavelength-optical intensity modulation techniques for fiber Bragg grating （FBG） sensors is presented. The performance is evaluated by measuring the strain and temperature. The first method consists of a narrow band source, an optical circulator, an FBG; and a power meter. The source and Bragg reflected signal from the FBG need to be matched to get linear results with good power levels. The source spectral power levels are very critical in this study. The power reflected from a matched reference FBG is fed into the measuring FBG in the second method. Since the FBGs are matched, the entire power is reflected back initially. During the measurement, the change in the measurand causes the reflected power from the sensing FBG to vary. A costly high resolution spectrum analyzer is required only during the characterization of the FBG and source. The performances of two interrogators are compared by measuring the strain and temperature. In the second method, the strain measurements can be made insensitive to the temperature variation by selecting a source with a flat spectrum at the measurement range. Highlights of these methods are the portability, cost effectiveness and better resolution.
基金supported by the National Natural Science Foundation of China (Nos. 51575015,51235001,51505013)
文摘Wireless interrogation of a rectangular microstrip patch antenna for strain measurement is investigated by simulations.To analyze the antenna performance,a microstrip line-feeding patch antenna at 10 GHz is designed.A patch antenna wirelessly fed by a horn is proposed to measure the strain.The direction information of strain detected by the patch antenna is also considered.The strain can be detected both in the width and length directions.It is shown that the strain can be measured wirelessly using a standard horn antenna.This kind of wireless strain-sensing technique offers significant potential for wireless structural health monitoring(SHM),especially for high-end equipment.
基金supported by the 11th Five Years Key Programs for Science and Technology Development of China under Grant No. 2006BAK04B02Natural Science Foundation of Shandong Province under Grant No. 2006ZRC01022.
文摘A method to interrogate fiber Bragg grating vibration sensor by narrow line width light is demonstrated. The interrogation scheme takes advantage of the intensity modulation of narrow spectral bandwidth light, such as distributed feedback laser, when a reflection or transmission spectrum curve of an fiber Bragg grating （FBG） moves due to the strain which is applied on the sensor. The sensor＇s response to accelerating frequency and amplitude is measured by experiment. The factors which have impacts on the sensitivity of the interrogation system are also discussed.
文摘This paper aims to study the embodiment and directive function of Cooperative Principle in trial process. By analyzing dialogues in the sample trial transcript, the author will find out the embodiment of Cooperative Principle in trial language, and draw a conclusion that the Cooperative Principle is important to direct the judge to discriminate if the suspect is cooperative, which helps the interrogation succeed.
基金This work was supported by the Education and Teaching Reform Research Project of Jiangsu Police Institute(No.2022B12)Qinglan Project for Jiangsu Province。
文摘The advent of the digital age has pointed out the direction for the development of higher education.In this paper,the undergraduate course“Science of Interrogation”is taken as the research subject,the challenges of big data teaching reform in universities are analyzed systematically,and concrete measures,such as adhering to the concept of teaching reform under the condition of big data,establishing professional teaching teams,improving the curriculum construction level,emphasizing on being student-centered,and optimizing the reform of the curriculum evaluation system,are proposed to address these challenges.
基金Shanghai"Xinglin Rising Star"Program(ZY(2018-2020)-RCPY-3018)Shanghai Municipal Administration of Traditional Chinese Medicine,Project of Science and Technology Commission of Changning District,Shanghai[CNKW2020Y24]National Natural Science Foundation of China(82074581).
文摘Patients with tinnitus are associated with adverse psychological reactions to varying degrees.Psychological interventions are currently the primary treatment to help patients eliminate negative responses to tinnitus.Traditional Chinese medicine uses four diagnostic methods or consultations.Interrogation is one such method.Through consultation,doctors can analyze the causes of tinnitus in patients,collect an accurate description,and assess the severity of the condition.At the same time,when doctors ask about other symptoms in a guided manner,they help patients refocus their attention to the symptoms so that their perception of tinnitus is changed,the vicious circular thinking patterns are broken,and their quality of life improves.When the patient understands why the physician establishes a medical plan,they are willing to cooperate actively and participate in a constructive doctor-patient relationship.This article analyzes the clinical methods and techniques of diagnosing tinnitus patients from the vantage of psychosomatic medicine,which has particular significance for the clinical treatment of tinnitus.
文摘Emily Dickinson is widely regarded as one of American's premier poets of the nineteenth century.In both style and content,her verse was,"revolutionary" in her day.Owing to the deep exploration of death and immortality,Emily Dickinson has become a puzzle in literary.As show in her poems,Dickinson's spiritual journey led her from na?ve nature-mysticism through disappointment,to a sacramental approach to immortality afterlife and further discouragement,culminating in a mature attitude of faithful unknowing and even more interrogating the immortality.This paper will base on the theory of the death and immortality through common people's fear to death,the essence of death,the significance of death to show the poetess's whole life-childhood,love,life condition,spiritual contradiction,and mainly her skeptical concept of death and immortality.
