A partition model of interference efficiency was constructed to study the coupling interference effect under combined internal and external flow.The concept of“internal flow efficiency”,“velocity ratio”and“interf...A partition model of interference efficiency was constructed to study the coupling interference effect under combined internal and external flow.The concept of“internal flow efficiency”,“velocity ratio”and“interference efficiency”were introduced to quantify the effect of internal flow and interference,and reveal the coupling mechanism among internal flow,external flow and interference effect.The results showed that the dynamic response of risers under variable angles was significantly different after considering the effect of internal flow.When the external flow velocity was smaller than 0.25 m/s,the vibration of risers was promoted by the internal flow.With the increase of external flow velocity,the effect of internal flow was weakened and the dynamic response of riser mainly depended on the external flow and interference effect.Under the effect of different internal flow,the interference efficiency had similar change trend.The interference effect amplified the complex secondary flow effect inside the riser,making the dynamic response of riser complex and random.In this paper,the overlap area and subdivision criterion of interference effect were constructed within the range of experimental velocity ratio,and the change curve of interference efficiency was obtained with an average meaning,which may have important practical meaning.展开更多
Collisional quantum interference （CQI） was observed in the intramolecular rotational energy transfer in the experiment of the static cell, and the integral interference angles were measured. To observe more precise ...Collisional quantum interference （CQI） was observed in the intramolecular rotational energy transfer in the experiment of the static cell, and the integral interference angles were measured. To observe more precise information, the experiment in the molecular beam should be taken,from which the relationship between the differential interference angle and the scattering angle can be obtained. In this paper, the theoretical model of CQI is described in an atom-diatom system in the condition of the molecular beam, based on the first-Born approximation of time-dependent perturbation theory, taking into accounts the long-range interaction potential. The method of observing and measuring correctly the differential interference angle is presented. The changing tendency of the differential interference angle with the impact parameter and relative velocity is discussed. The changing tendencies of the differential interference angle with the parameter of experiment in the molecular beam, including the impact parameter and the velocity are discussed. This theoretical model is important to understand or perform the experiment in the molecular beam.展开更多
Young’s double-slit experiment shows characteristics of light that are modeled as indicating a wave nature of light. Other experiments suggest a particle model of light. An experiment is performed with the Fraunhofer...Young’s double-slit experiment shows characteristics of light that are modeled as indicating a wave nature of light. Other experiments suggest a particle model of light. An experiment is performed with the Fraunhofer pattern from a first mask impinging on a transparent second mask with a slit. The screen pattern is an interference pattern such as produced in Young’s Experiment. An opaque strip between the first and second mask blocks the light of the center maxima from the first mask. The screen interference fringes remained. Moving the glass mask so the slit is removed from light shows the necessity of a slit in the second mask. This suggests a Newtonian type of light model. This experiment rejects the wave models of light.展开更多
This study investigated the feasibility of replacing urinary epithelial cells with oral keratinocytes and transforming growth factor-β1 （TGF-β1） small interfering RNA （siRNA）-transfected fibroblasts seeded on bl...This study investigated the feasibility of replacing urinary epithelial cells with oral keratinocytes and transforming growth factor-β1 （TGF-β1） small interfering RNA （siRNA）-transfected fibroblasts seeded on bladder acellular matrix graft （BAMG） in order to reconstruct tissue-engineered urethra. Constructed siRNAs, which expressed plasmids targeting TGF-β1, were transfected into rabbit fibroblasts. The effective siRNA was screened out by RT-PCR and was transfected into rabbit fibroblasts again. Synthesis of type I collagen in culture medium was measured by enzyme-linked immuno sorbent assay （ELISA）. Autologous oral keratinocytes and TGF-β1 siRNA-transfected fibroblasts were seeded onto BAMGs to obtain a tissue-engineered mucosa. The tissue-engineered mucosa was assessed morphologically and with the help of scanning electron microscopy. The TGF-β1 siRNA decreased the expression of fibroblasts synthesis type I collagen. Oral keratinocytes and TGF-β1 siRNA-transfected fibroblasts were seeded onto sterilized BAMG to obtain a tissue-engineered mucosa for urethral reconstruction. The compound graft was assessed using scanning electron microscope. Oral keratinocytes and TGF-β1 siRNA-transfected fibroblasts had a good compatibility with BAMG. The downregulation of fibroblasts synthesis type I collagen expression by constructed siRNA interfering TGF-β1 provided a potential basis for genetic therapy of urethral scar. Oral keratinocytes and TGF-β1 siRNA-transfected fibroblasts had good compatibility with BAMG and the compound graft could be a new choice for urethral reconstruction.展开更多
The“riser group−fluid between risers”is taken as the carrier,and the experiment on vortex-induced vibration of tandem riser groups coupling interference effect under sensitive spacing is performed.The least-square m...The“riser group−fluid between risers”is taken as the carrier,and the experiment on vortex-induced vibration of tandem riser groups coupling interference effect under sensitive spacing is performed.The least-square method is used to linearly fit the reduced velocity and main frequency,and the rule of Strouhal numbers is analyzed.Each mode is separated based on the mode decomposition theory,and the mode conversion mechanism is also explored.The concept of“interference efficiency”is introduced to study the dynamic characteristics and response evolutions of different riser groups.The results show that the wake shielding effect widely exists in tandem riser groups,and the interference effect of midstream and downstream risers on their upstream risers is significantly lower than that of upstream risers on midstream and downstream risers.The trajectories of midstream and downstream risers lag behind their upstream risers due to multiple shadowing effects,the vibration frequency range of downstream riser is widened and the dominant frequency is extremely unstable.Compared with the isolated riser,wake interference suppresses the vibration dis-placement of the midstream and downstream risers in the in-line direction,and enhances the displacement of upstream and midstream risers in the cross-flow direction.The interference effect of the fluid between risers at low velocities is stronger than that at higher velocities,and the cross-flow displacements of upstream risers are always in the interference enhancement region.It is urgent to pay attention to the cross-flow displacement of upstream and midstream risers in tandem riser groups considering the safety design.展开更多
During advanced water detection using the transient electromagnetic method, the exploration effect for water-rich area is often poor due to the interference of bolts that are distributed in different positions in work...During advanced water detection using the transient electromagnetic method, the exploration effect for water-rich area is often poor due to the interference of bolts that are distributed in different positions in working face. Thus, the study on the interference characteristics of bolts in different states has important directive significance for improving the acquisition quality and data processing method in water detection. Based on the analysis of the distribution laws of magnetic field excited by small multi-turn coincident loop in full space of homogeneity, the test on the interference of bolts has been designed in the mine. Through drilling 18 holes around the overlapping coil in the working face, mass data are collected in order with the posi- tion change and the exposed bolt length. The results of comprehensive data analysis show that the transient electromagnetic field is strongly interfered as the distance between the bolt and the center of the coil is less than 3 m, and the interference varies greatly as the distance varies. On the other hand, the field induced by the bolts can be ignored as the distance exceeds 3 m. The findings can help to improve data acquisition and correction during advanced water detection when using the transient electromagnetic method.展开更多
in this paper a method is outlined to compute wall-interferenee in closedlow speed wind tunnel for 3D high lift test using wall pressure at optimum points andthe influence function (WPIF method for short). The experim...in this paper a method is outlined to compute wall-interferenee in closedlow speed wind tunnel for 3D high lift test using wall pressure at optimum points andthe influence function (WPIF method for short). The experimental results of a high liftmodel in small wind tunnel are corrected by applying the WPIF method. The correctedresults are compared with the wall interference-free data of this high lift model in largewind tunnel. It is shown that the WPIF method is desirable for the correction of lift,drag and pitch moment.展开更多
The effect of the size of a delta wing relative to that of the test section on the vortex breakdown location over a delta wing oscillating in pitch to very high angles of attack was ...The effect of the size of a delta wing relative to that of the test section on the vortex breakdown location over a delta wing oscillating in pitch to very high angles of attack was investigated experimentally using flow visualization. The unsteady wall pressure characteristics such as delay, frequency were analyzed. An unsteady tunnel wall correction, applying influence functions in steady wall pressure correction method and unsteady wall pressure at the optimum points, was presented. Experimental examinations prove that the unsteady tunnel wall correction is desirable.展开更多
A vortex-induced vibration(VIV)experiment of rough risers with coupling interference effect under a side-by-side arrangement was carried out in a wave-current combined flume.The roughness of the riser was characterize...A vortex-induced vibration(VIV)experiment of rough risers with coupling interference effect under a side-by-side arrangement was carried out in a wave-current combined flume.The roughness of the riser was characterized by arranging different specifications of surface attachments on the surface of the riser.Rough risers with three different roughnesses were arranged side by side with smooth risers to explore the VIV response of the riser under the combined action of roughness and interference effect,and to reveal the coupling mechanism between roughness and interference effect.The experimental results show that,compared with that of a smooth riser,the VIV of a rough riser under the coupling interference effect has a wider"lock-in"region,and the displacement decreases more significantly at a high reduced velocity,which is more likely to excite higher-order modes and frequency responses.In addition,the displacement response and frequency response of the smooth riser are not significantly affected by wake interference from the rough riser,which is caused by the decrease of the wake region due to the delay of the boundary layer separation point of the rough riser.展开更多
In order to study the response law of vortex-induced vibration(VIV)of marine risers under the combined action of roughness and interference effects,and to reveal the coupling mechanism of roughness and interference ef...In order to study the response law of vortex-induced vibration(VIV)of marine risers under the combined action of roughness and interference effects,and to reveal the coupling mechanism of roughness and interference effects on the riser,a VIV experiment of rough risers in tandem arrangement was conducted in a wave−current combined flume.The experiment characterized the risers’roughness by arranging different specifications of attachments on the surface of the risers.Three rough risers with different roughness and smooth risers were arranged in tandem arrangement,with the rough risers arranged downstream.The experimental results indicate that the suppression of the attachments on the downstream risers’vibration are more significant both in the CF and IL directions as the reduced velocity increases.For the downstream riser,the amplitude response of rough riser is more significantly weakened compared with the smooth one at high reduced velocity.For the upstream risers,changes in the roughness and spacing ratio have an impact on their‘lock-in’region.When the roughness of downstream risers is relatively large(0.1300)and the spacing between risers is small(S/D=4.0),the reduced velocity range of‘lock-in’region in the CF direction of upstream risers is obviously expanded,and the displacement in the‘lock-in’region is severer.展开更多
After remanufacturing disassembly,several kinds of friction damages can be found on the mating surface of interference fit.These damages should be repaired and the cost is closely related to the severity of damages.In...After remanufacturing disassembly,several kinds of friction damages can be found on the mating surface of interference fit.These damages should be repaired and the cost is closely related to the severity of damages.Inspired by the excellent performance of surface texture in wear reduction,5 shapes of pit array textures are added to the specimens’surface to study their reduction effect of disassembly damage for interference fit.The results of disassembly experiments show that the order of influence of texture parameters on disassembly damage is as follows:equivalent circle diameter of single texture,texture shape and texture surface density.The influence of equivalent circle diameter of single texture and texture shape are obviously more significant than that of texture surface density.The circular texture with a surface density of 30%and a diameter of 100μm shows an excellent disassembly damage reduction effect because of its perfect ability of abrasive particle collection.And the probability of disassembly damage formation and evolution is also relatively small on this kind of textured surface.Besides,the load-carrying capacity of interference fit with the excellent texture is confirmed by load-carrying capacity experiments.The results show that the load-carrying capacity of the excellent texture surface is increased about 40%compared with that of without texture.This research provides a potential approach to reduce disassembly damage for interference fit.展开更多
Taking the three-riser group arranged in tandem as the research subject,an experimental study was carried out on the risers arranged in tandem.The purpose is to explore the sensitivity of the dynamic response of each ...Taking the three-riser group arranged in tandem as the research subject,an experimental study was carried out on the risers arranged in tandem.The purpose is to explore the sensitivity of the dynamic response of each riser to spacing ratio and reveal the physical mechanism of riser groups under the interference effect.The spacing ratios of the adj acent risers are 4.0,5.0,6.0,and 8.0.At the spacing between the risers of 4.0D,the strong feedback effect increases the cross-flow(CF) displacement amplitude of the upstream riser.The shielding effect is the key factor affecting the interference effect on the midstream and downstream risers.At low reduced velocities,the shielding area initially appears,the displacement amplitude of the midstream and downstream risers varies greatly,the vibration of the two risers is still dominated by the first-order mode,and the transition between adjacent vibration modes is restrained.The multi-frequency superposition phenomenon is very significant at high reduced velocities.The most sensitive interference spacing under the test conditions is obtained.Due to the separation of the incoming flow and the double shielding effect of the upstream and midstream risers,the regular vortex-induced vibration in the wake area of the downstream riser is broken,and the vibration in the two directions is weakened.In general,the interference effect is more significant for the CF vibration of the three-riser groups than the in-line(IL) vibration.展开更多
Objective: To explore the inhibition of the expression of c-Myc in human hepatocellular carcinoma via vector-based RNA interference (RNAi). Methods: Two RNA interference DNA templates targeting c-Myc oncogene were des...Objective: To explore the inhibition of the expression of c-Myc in human hepatocellular carcinoma via vector-based RNA interference (RNAi). Methods: Two RNA interference DNA templates targeting c-Myc oncogene were designed via online software and synthesized. By ligation, the fragments were inserted into pSilencer 1.0-U6 to construct the recombinant siRNA expressing plasmids. The identified recombinants were introduced into BEL-7402 cells with Lipofactamine. The inhibition of c-Myc expression, together with the expression of CDK4, hTERT and Gadd45β in c-Myc down-regulated BEL-7402 cells, were analyzed by semi-quantitative RT-PCR. Results: Two recombinant plasmids pSic-myc-1 and pSic-myc-2, which direct the yields of siRNAs targeting c-Myc in cells, were constructed. Among which, pSic-myc-2 was shown to trigger a RNAi-mediated inhibition of expression of c-Myc in BEL-7402 by up to 90%. In c-Myc knockdown BEL-7402 cells, the expression of CDK4 and hTERT were down-regulated with a ratio of 85% and 57%, respectively, while the expression of Gadd45β was up-regulated by up to 110%. Conclusion: The expression of c-Myc in BEL-7402 could be suppressed by vector-based RNA interference successfully. The knockdown of c-Myc in turn resulted in the changes of expression of genes related to cell proliferation and apoptosis. Thus, our study provided a preliminary data in searching of a c-Myc-targeted RNAi therapy of human hepatocellular carcinoma.展开更多
Transitioning from outcrossing to self-fertilization is a widespread reproductive strategy in plants,especially in environments where pollination is limited.Despite its prevalence,this transition has rarely been exami...Transitioning from outcrossing to self-fertilization is a widespread reproductive strategy in plants,especially in environments where pollination is limited.Despite its prevalence,this transition has rarely been examined using transplant experiments,and previous studies have overlooked the contribution of the male parent in elucidating mating diversity.In this study,six transplanted populations were generated to investigate the relationship of the pollination environment with plant mating patterns and fecundity in Primula oreodoxa,a species that exhibits both distyly(predominantly outcrossing)and homostyly(predominantly selfing),based on data from 3582 individuals and 11 SSR markers.Homostylous plants had fruit and seed sets comparable to those of distylous plants at lower elevations but exhibited a clear reproductive advantage at higher elevations,particularly compared with the S morph.As elevation increased,the populational selfing rates increased,and the genetic diversity among the progeny was reduced.Furthermore,the visitation frequency of long-tongued pollinators was negatively and positively correlated with the selfing rate and number of mates,respectively,in the L and S morphs.In contrast,short-tongued pollinator visitation showed opposite correlations with the selfing rate and number of mates in homostylous morphs.In most populations,individuals functioned consistently as both female and male,and mating occurred randomly,suggesting a breakdown of the distyly polymorphism.Overall,our results provide experimental validation of the reproductive advantages of homostyly at high elevations by revealing that pollinator visitation shapes the selfing rate and mating diversity within populations,potentially driving the divergence of mating systems along environmental gradients.展开更多
Pathological scarring,manifested in the form of hypertrophic scars(HTS)and keloid scars(KS),represents a major clinical challenge due to its aesthetic and functional implications for patients.Understanding the molecul...Pathological scarring,manifested in the form of hypertrophic scars(HTS)and keloid scars(KS),represents a major clinical challenge due to its aesthetic and functional implications for patients.Understanding the molecular mechanisms involved in these types of scars and developing effective treatments requires the use of controlled ex-perimental models,especially animals,to overcome the limitations of clinical studies.The aim of this sistematic review is to critically analyze the animal models used in the last five years(2020-2025)for the study of pathological scars,highlighting their advantages,limitations and applicability in the development of new therapeutic strat-egies.Murine,rabbit and porcine models,as well as alternative models,offer varied perspectives on the formation and treatment of HTS and KS,with an emphasis on histological and molecular correlations with human pathology.By synthesizing recent data,the paper highlights the essential role of preclinical research in optimizing an-tifibrotic treatments and in advancing the translation of data into the clinical sphere.Overall,animal models remain essential for bridging mechanistic insights with clinical translation,supporting the development of more effective and personalized anti-scar therapies.展开更多
A method for correlating thermal light over a wide spectral range is proposed.A multi-wavelength pseudothermal source,prepared by projecting laser beams of multiple wavelengths(650 nm,635 nm,532 nm,and 473 nm)onto a m...A method for correlating thermal light over a wide spectral range is proposed.A multi-wavelength pseudothermal source,prepared by projecting laser beams of multiple wavelengths(650 nm,635 nm,532 nm,and 473 nm)onto a moving thin ground glass plate,is employed in a double-slit interference experiment.The ground glass plate induces random phase differences between light beams of different wavelengths passing through it.This initial random phase difference significantly influences the high-order intensity correlation functions of multi-wavelength thermal beams.Experimentally,second-order correlated interference patterns,including subwavelength interference,of pseudothermal beams with different wavelengths are observed in the intensity correlation measurements.This method facilitates applications of correlated thermal photons in quantum information processing and quantum imaging.展开更多
With the rapid development of intelligent electronic and military equipment,multifunctional flexible materials that integrat electromagnetic interference(EMI)shielding,temperature sensing,and information encryption ar...With the rapid development of intelligent electronic and military equipment,multifunctional flexible materials that integrat electromagnetic interference(EMI)shielding,temperature sensing,and information encryption are urgently required.This study presents a bio-inspired hierarchical composite foam fabricated using supercritical nitrogen foaming technology.This material exhibits a honeycomb structure,with pore cell sizes controllable within a range of 30–92μm by regulating the filler.The carbon fiber felt(CFf)provides efficient reflection of electromagnetic waves,while the chloroprene rubber/carbon fiber/carbon black foam facilitates both wave absorption and temperature monitoring through its optimized conductive network.This synergistic mechanism results in an EMI shielding effectiveness(SE)of 60.06 d B with excellent temperature sensing performance(The temperature coefficient of resistance(TCR)is-2.642%/℃)in the 24–70℃ range.Notably,the material has a thermal conductivity of up to 0.159 W/(m·K),and the bio-inspired layered design enables information encryption,demonstrating the material's potential for secure communication applications.The foam also has tensile properties of up to 5.13 MPa and a tear strength of 33.02 N/mm.This biomimetic design overcomes the traditional limitations of flexible materials and provides a transformative solution for next-generation applications such as flexible electronics,aerospace systems and military equipment,which urgently need integrated electromagnetic protection,thermal management and information security.展开更多
High-precision optical frequency measurement serves as a cornerstone of modern science and technology,enabling advancements in fields ranging from fundamental physics to quantum information technologies.Obtaining prec...High-precision optical frequency measurement serves as a cornerstone of modern science and technology,enabling advancements in fields ranging from fundamental physics to quantum information technologies.Obtaining precise photon frequencies,especially in the ultraviolet or even extreme ultraviolet regimes,is a key goal in both light–matter interaction experiments and engineering applications.High-order harmonic generation(HHG)is an ideal light source for producing such photons.In this work,we propose an optical temporal interference model(OTIM)that establishes an analogy with multi-slit Fraunhofer diffraction(MSFD)to manipulate fine-frequency photon generation by exploiting the temporal coherence of HHG processes.Our model provides a unified physical framework for three distinct non-integer HHG generation schemes:single-pulse,shaped-pulse,and laser pulse train approaches,which correspond to single-MSFD-like,double-MSFD-like,and multi-MSFD-like processes,respectively.Arbitrary non-integer HHG photons can be obtained using our scheme.Our approach provides a new perspective for accurately measuring and controlling photon frequencies in fields such as frequency comb technology,interferometry,and atomic clocks.展开更多
Recovered samples of Jilin H5 chondrite experimentally shocked to 12-133 GPa were studied to explore the behavior of opaque minerals under shock loading using SEM-EDS,Raman spectroscopy,and TIMA.The following results ...Recovered samples of Jilin H5 chondrite experimentally shocked to 12-133 GPa were studied to explore the behavior of opaque minerals under shock loading using SEM-EDS,Raman spectroscopy,and TIMA.The following results were obtained.Firstly,at pressures lower than 53GPa,the opaque minerals still keep the unmelted state,while at 78 GPa and higher,FeNi metal and troilite form eutectic intergrowths occurring as disorderly fine veinlets filling the shock-induced fractures in silicate minerals.Secondly,single kamacite grains still maintain their contour at 12 GPa,but a part of brittle troilite grains was fragmented and squeezed into the shock-induced fractures within kamacite grains.At53 and 133 GPa,many more troilite fragments are poured in the kamacite interior to form disordered hybrid aggregates or to form squiggly strips,respectively.Similar phenomena are observed within single troilite grains,but the mineral squeezed into troilite grains is kamacite.Thirdly,chromite is a hard and refractory oxide mineral.When the shock pressure rises step by step from 12 to 133 GPa,the shock effect of chromite is only fragmentation.Its grain size decreases from tens of um at 53 GPa to a few um at 133 GPa.And,fourthly,native copper exhibits distinct redistribution behavior at high temperature.In Jilin samples shock-loaded to 12 GPa,copper initially located at troilite-kamacite interfaces partially transferred into small troilite grains containing fine FeNi particles.At 53 and 133 GPa,native copper preferentially transferred into larger troilite grains containing more particles of eutectic FeNi metal.展开更多
Brain lesions,such as those caused by stroke or traumatic brain injury(TBI),frequently result in persistent motor and cognitive impairments that significantly affect the individual patient's quality of life.Despit...Brain lesions,such as those caused by stroke or traumatic brain injury(TBI),frequently result in persistent motor and cognitive impairments that significantly affect the individual patient's quality of life.Despite differences in the mechanisms of injury,both conditions share a high prevalence of motor and cognitive impairments.These deficits show only limited natural recovery.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.51709161 and U2006226)the Key Technology Research and Development Program of Shandong Province(Grant No.2019GHY112061)+1 种基金the Natural Science Foundation of Shandong Province(Grant No.ZR2022QE118)the Youth Talent Introduction and Cultivation Program of College in Shandong Province。
文摘A partition model of interference efficiency was constructed to study the coupling interference effect under combined internal and external flow.The concept of“internal flow efficiency”,“velocity ratio”and“interference efficiency”were introduced to quantify the effect of internal flow and interference,and reveal the coupling mechanism among internal flow,external flow and interference effect.The results showed that the dynamic response of risers under variable angles was significantly different after considering the effect of internal flow.When the external flow velocity was smaller than 0.25 m/s,the vibration of risers was promoted by the internal flow.With the increase of external flow velocity,the effect of internal flow was weakened and the dynamic response of riser mainly depended on the external flow and interference effect.Under the effect of different internal flow,the interference efficiency had similar change trend.The interference effect amplified the complex secondary flow effect inside the riser,making the dynamic response of riser complex and random.In this paper,the overlap area and subdivision criterion of interference effect were constructed within the range of experimental velocity ratio,and the change curve of interference efficiency was obtained with an average meaning,which may have important practical meaning.
基金The project supported by National Natural Science Foundation of China under Grant No. 10374040 and Fund of the Department of Education of Liaoning Province of China under Grant No. 2004 F 004 . Thanks are given to Dr. Sun Meng-Tao （Department of Chemical Physics, Lund University, Lund, Sweden） due to his help in this work.
文摘Collisional quantum interference （CQI） was observed in the intramolecular rotational energy transfer in the experiment of the static cell, and the integral interference angles were measured. To observe more precise information, the experiment in the molecular beam should be taken,from which the relationship between the differential interference angle and the scattering angle can be obtained. In this paper, the theoretical model of CQI is described in an atom-diatom system in the condition of the molecular beam, based on the first-Born approximation of time-dependent perturbation theory, taking into accounts the long-range interaction potential. The method of observing and measuring correctly the differential interference angle is presented. The changing tendency of the differential interference angle with the impact parameter and relative velocity is discussed. The changing tendencies of the differential interference angle with the parameter of experiment in the molecular beam, including the impact parameter and the velocity are discussed. This theoretical model is important to understand or perform the experiment in the molecular beam.
文摘Young’s double-slit experiment shows characteristics of light that are modeled as indicating a wave nature of light. Other experiments suggest a particle model of light. An experiment is performed with the Fraunhofer pattern from a first mask impinging on a transparent second mask with a slit. The screen pattern is an interference pattern such as produced in Young’s Experiment. An opaque strip between the first and second mask blocks the light of the center maxima from the first mask. The screen interference fringes remained. Moving the glass mask so the slit is removed from light shows the necessity of a slit in the second mask. This suggests a Newtonian type of light model. This experiment rejects the wave models of light.
基金This work was supported by the National Natural Science Foundation of China （No. 30901503）.
文摘This study investigated the feasibility of replacing urinary epithelial cells with oral keratinocytes and transforming growth factor-β1 （TGF-β1） small interfering RNA （siRNA）-transfected fibroblasts seeded on bladder acellular matrix graft （BAMG） in order to reconstruct tissue-engineered urethra. Constructed siRNAs, which expressed plasmids targeting TGF-β1, were transfected into rabbit fibroblasts. The effective siRNA was screened out by RT-PCR and was transfected into rabbit fibroblasts again. Synthesis of type I collagen in culture medium was measured by enzyme-linked immuno sorbent assay （ELISA）. Autologous oral keratinocytes and TGF-β1 siRNA-transfected fibroblasts were seeded onto BAMGs to obtain a tissue-engineered mucosa. The tissue-engineered mucosa was assessed morphologically and with the help of scanning electron microscopy. The TGF-β1 siRNA decreased the expression of fibroblasts synthesis type I collagen. Oral keratinocytes and TGF-β1 siRNA-transfected fibroblasts were seeded onto sterilized BAMG to obtain a tissue-engineered mucosa for urethral reconstruction. The compound graft was assessed using scanning electron microscope. Oral keratinocytes and TGF-β1 siRNA-transfected fibroblasts had a good compatibility with BAMG. The downregulation of fibroblasts synthesis type I collagen expression by constructed siRNA interfering TGF-β1 provided a potential basis for genetic therapy of urethral scar. Oral keratinocytes and TGF-β1 siRNA-transfected fibroblasts had good compatibility with BAMG and the compound graft could be a new choice for urethral reconstruction.
基金This work was supported by the National Natural Science Foundation of China(Grant Nos.51709161 and U2006226)the Key Technology Research and Development Program of Shandong Province(Grant No.2019GHY112061)+1 种基金the Research and Innovation Team of Ocean Oil and Gas Development Engineering Structure,College of Civil Engineering and Architecture,Shandong University of Science and Technology(Grant No.2019TJKYTD01)the Natural Science Foundation of Shandong Province(Grant No.ZR2020ME261).
文摘The“riser group−fluid between risers”is taken as the carrier,and the experiment on vortex-induced vibration of tandem riser groups coupling interference effect under sensitive spacing is performed.The least-square method is used to linearly fit the reduced velocity and main frequency,and the rule of Strouhal numbers is analyzed.Each mode is separated based on the mode decomposition theory,and the mode conversion mechanism is also explored.The concept of“interference efficiency”is introduced to study the dynamic characteristics and response evolutions of different riser groups.The results show that the wake shielding effect widely exists in tandem riser groups,and the interference effect of midstream and downstream risers on their upstream risers is significantly lower than that of upstream risers on midstream and downstream risers.The trajectories of midstream and downstream risers lag behind their upstream risers due to multiple shadowing effects,the vibration frequency range of downstream riser is widened and the dominant frequency is extremely unstable.Compared with the isolated riser,wake interference suppresses the vibration dis-placement of the midstream and downstream risers in the in-line direction,and enhances the displacement of upstream and midstream risers in the cross-flow direction.The interference effect of the fluid between risers at low velocities is stronger than that at higher velocities,and the cross-flow displacements of upstream risers are always in the interference enhancement region.It is urgent to pay attention to the cross-flow displacement of upstream and midstream risers in tandem riser groups considering the safety design.
基金Supported by the Key Projects of Anhui Provincial Scientific and Technological Program （11010401015） the Key Program of National Natural Science Foundation of China （51134012）
文摘During advanced water detection using the transient electromagnetic method, the exploration effect for water-rich area is often poor due to the interference of bolts that are distributed in different positions in working face. Thus, the study on the interference characteristics of bolts in different states has important directive significance for improving the acquisition quality and data processing method in water detection. Based on the analysis of the distribution laws of magnetic field excited by small multi-turn coincident loop in full space of homogeneity, the test on the interference of bolts has been designed in the mine. Through drilling 18 holes around the overlapping coil in the working face, mass data are collected in order with the posi- tion change and the exposed bolt length. The results of comprehensive data analysis show that the transient electromagnetic field is strongly interfered as the distance between the bolt and the center of the coil is less than 3 m, and the interference varies greatly as the distance varies. On the other hand, the field induced by the bolts can be ignored as the distance exceeds 3 m. The findings can help to improve data acquisition and correction during advanced water detection when using the transient electromagnetic method.
文摘in this paper a method is outlined to compute wall-interferenee in closedlow speed wind tunnel for 3D high lift test using wall pressure at optimum points andthe influence function (WPIF method for short). The experimental results of a high liftmodel in small wind tunnel are corrected by applying the WPIF method. The correctedresults are compared with the wall interference-free data of this high lift model in largewind tunnel. It is shown that the WPIF method is desirable for the correction of lift,drag and pitch moment.
文摘The effect of the size of a delta wing relative to that of the test section on the vortex breakdown location over a delta wing oscillating in pitch to very high angles of attack was investigated experimentally using flow visualization. The unsteady wall pressure characteristics such as delay, frequency were analyzed. An unsteady tunnel wall correction, applying influence functions in steady wall pressure correction method and unsteady wall pressure at the optimum points, was presented. Experimental examinations prove that the unsteady tunnel wall correction is desirable.
基金financially supported by the Natural Science Foundation of Shandong Province(Grant Nos.ZR2023ME040 and ZR2022QE118)the Key Technology Research and Development Program of Shandong Province(Grant No.2023CXGC010316)+1 种基金the Natural Science Foundation of Qingdao(Grant No.23-2-1-207-zyyd-jch)the Introduction and Education Plan for Young Innovative talents in Colleges and Universities of Shandong Province(Marine Civil Engineering Materials and Structure Innovation Research Team).
文摘A vortex-induced vibration(VIV)experiment of rough risers with coupling interference effect under a side-by-side arrangement was carried out in a wave-current combined flume.The roughness of the riser was characterized by arranging different specifications of surface attachments on the surface of the riser.Rough risers with three different roughnesses were arranged side by side with smooth risers to explore the VIV response of the riser under the combined action of roughness and interference effect,and to reveal the coupling mechanism between roughness and interference effect.The experimental results show that,compared with that of a smooth riser,the VIV of a rough riser under the coupling interference effect has a wider"lock-in"region,and the displacement decreases more significantly at a high reduced velocity,which is more likely to excite higher-order modes and frequency responses.In addition,the displacement response and frequency response of the smooth riser are not significantly affected by wake interference from the rough riser,which is caused by the decrease of the wake region due to the delay of the boundary layer separation point of the rough riser.
基金supported by the Natural Science Foundation of Shandong Province(Grant Nos.ZR2023ME040 and ZR2022QE118)the Key Technology Research and Development Program of Shandong Province(Grant No.2023CXGC010316)the Natural Science Foundation of Qingdao(Grant No.23-2-1-207-zyyd-jch),and the National Natural Science Foundation of China(Grant No.51709161).
文摘In order to study the response law of vortex-induced vibration(VIV)of marine risers under the combined action of roughness and interference effects,and to reveal the coupling mechanism of roughness and interference effects on the riser,a VIV experiment of rough risers in tandem arrangement was conducted in a wave−current combined flume.The experiment characterized the risers’roughness by arranging different specifications of attachments on the surface of the risers.Three rough risers with different roughness and smooth risers were arranged in tandem arrangement,with the rough risers arranged downstream.The experimental results indicate that the suppression of the attachments on the downstream risers’vibration are more significant both in the CF and IL directions as the reduced velocity increases.For the downstream riser,the amplitude response of rough riser is more significantly weakened compared with the smooth one at high reduced velocity.For the upstream risers,changes in the roughness and spacing ratio have an impact on their‘lock-in’region.When the roughness of downstream risers is relatively large(0.1300)and the spacing between risers is small(S/D=4.0),the reduced velocity range of‘lock-in’region in the CF direction of upstream risers is obviously expanded,and the displacement in the‘lock-in’region is severer.
基金Supported by National Natural Science Foundation of China (Grant No.51405121)。
文摘After remanufacturing disassembly,several kinds of friction damages can be found on the mating surface of interference fit.These damages should be repaired and the cost is closely related to the severity of damages.Inspired by the excellent performance of surface texture in wear reduction,5 shapes of pit array textures are added to the specimens’surface to study their reduction effect of disassembly damage for interference fit.The results of disassembly experiments show that the order of influence of texture parameters on disassembly damage is as follows:equivalent circle diameter of single texture,texture shape and texture surface density.The influence of equivalent circle diameter of single texture and texture shape are obviously more significant than that of texture surface density.The circular texture with a surface density of 30%and a diameter of 100μm shows an excellent disassembly damage reduction effect because of its perfect ability of abrasive particle collection.And the probability of disassembly damage formation and evolution is also relatively small on this kind of textured surface.Besides,the load-carrying capacity of interference fit with the excellent texture is confirmed by load-carrying capacity experiments.The results show that the load-carrying capacity of the excellent texture surface is increased about 40%compared with that of without texture.This research provides a potential approach to reduce disassembly damage for interference fit.
基金financially supported by the National Natural Science Foundation of China (Grant No. 42177167)the Natural Science Foundation of Shandong Province (Grant No. ZR2019QEE008)。
文摘Taking the three-riser group arranged in tandem as the research subject,an experimental study was carried out on the risers arranged in tandem.The purpose is to explore the sensitivity of the dynamic response of each riser to spacing ratio and reveal the physical mechanism of riser groups under the interference effect.The spacing ratios of the adj acent risers are 4.0,5.0,6.0,and 8.0.At the spacing between the risers of 4.0D,the strong feedback effect increases the cross-flow(CF) displacement amplitude of the upstream riser.The shielding effect is the key factor affecting the interference effect on the midstream and downstream risers.At low reduced velocities,the shielding area initially appears,the displacement amplitude of the midstream and downstream risers varies greatly,the vibration of the two risers is still dominated by the first-order mode,and the transition between adjacent vibration modes is restrained.The multi-frequency superposition phenomenon is very significant at high reduced velocities.The most sensitive interference spacing under the test conditions is obtained.Due to the separation of the incoming flow and the double shielding effect of the upstream and midstream risers,the regular vortex-induced vibration in the wake area of the downstream riser is broken,and the vibration in the two directions is weakened.In general,the interference effect is more significant for the CF vibration of the three-riser groups than the in-line(IL) vibration.
基金Supported by grants from the Young Scientific and Technical InnovationFoundation of Fujian Province (No. 2004J067).
文摘Objective: To explore the inhibition of the expression of c-Myc in human hepatocellular carcinoma via vector-based RNA interference (RNAi). Methods: Two RNA interference DNA templates targeting c-Myc oncogene were designed via online software and synthesized. By ligation, the fragments were inserted into pSilencer 1.0-U6 to construct the recombinant siRNA expressing plasmids. The identified recombinants were introduced into BEL-7402 cells with Lipofactamine. The inhibition of c-Myc expression, together with the expression of CDK4, hTERT and Gadd45β in c-Myc down-regulated BEL-7402 cells, were analyzed by semi-quantitative RT-PCR. Results: Two recombinant plasmids pSic-myc-1 and pSic-myc-2, which direct the yields of siRNAs targeting c-Myc in cells, were constructed. Among which, pSic-myc-2 was shown to trigger a RNAi-mediated inhibition of expression of c-Myc in BEL-7402 by up to 90%. In c-Myc knockdown BEL-7402 cells, the expression of CDK4 and hTERT were down-regulated with a ratio of 85% and 57%, respectively, while the expression of Gadd45β was up-regulated by up to 110%. Conclusion: The expression of c-Myc in BEL-7402 could be suppressed by vector-based RNA interference successfully. The knockdown of c-Myc in turn resulted in the changes of expression of genes related to cell proliferation and apoptosis. Thus, our study provided a preliminary data in searching of a c-Myc-targeted RNAi therapy of human hepatocellular carcinoma.
基金funded by grants from the National Natural Science Foundation of China(31800314,32370239,U160323)the foundation of South China Botanical Garden,Chinese Academy of Sciences(QNXM-06)to SY and the Doctoral Research Foundation of China West Normal University(412994).
文摘Transitioning from outcrossing to self-fertilization is a widespread reproductive strategy in plants,especially in environments where pollination is limited.Despite its prevalence,this transition has rarely been examined using transplant experiments,and previous studies have overlooked the contribution of the male parent in elucidating mating diversity.In this study,six transplanted populations were generated to investigate the relationship of the pollination environment with plant mating patterns and fecundity in Primula oreodoxa,a species that exhibits both distyly(predominantly outcrossing)and homostyly(predominantly selfing),based on data from 3582 individuals and 11 SSR markers.Homostylous plants had fruit and seed sets comparable to those of distylous plants at lower elevations but exhibited a clear reproductive advantage at higher elevations,particularly compared with the S morph.As elevation increased,the populational selfing rates increased,and the genetic diversity among the progeny was reduced.Furthermore,the visitation frequency of long-tongued pollinators was negatively and positively correlated with the selfing rate and number of mates,respectively,in the L and S morphs.In contrast,short-tongued pollinator visitation showed opposite correlations with the selfing rate and number of mates in homostylous morphs.In most populations,individuals functioned consistently as both female and male,and mating occurred randomly,suggesting a breakdown of the distyly polymorphism.Overall,our results provide experimental validation of the reproductive advantages of homostyly at high elevations by revealing that pollinator visitation shapes the selfing rate and mating diversity within populations,potentially driving the divergence of mating systems along environmental gradients.
基金Ministry of Research,Innovation and Digitization,CCCDI-UEFISCDI,Grant/Award Number:PN-IV-P7-7.1-PED-2024-1578,within PNCDI Ⅳ.
文摘Pathological scarring,manifested in the form of hypertrophic scars(HTS)and keloid scars(KS),represents a major clinical challenge due to its aesthetic and functional implications for patients.Understanding the molecular mechanisms involved in these types of scars and developing effective treatments requires the use of controlled ex-perimental models,especially animals,to overcome the limitations of clinical studies.The aim of this sistematic review is to critically analyze the animal models used in the last five years(2020-2025)for the study of pathological scars,highlighting their advantages,limitations and applicability in the development of new therapeutic strat-egies.Murine,rabbit and porcine models,as well as alternative models,offer varied perspectives on the formation and treatment of HTS and KS,with an emphasis on histological and molecular correlations with human pathology.By synthesizing recent data,the paper highlights the essential role of preclinical research in optimizing an-tifibrotic treatments and in advancing the translation of data into the clinical sphere.Overall,animal models remain essential for bridging mechanistic insights with clinical translation,supporting the development of more effective and personalized anti-scar therapies.
基金supported by the National Natural Science Foundation of China(Grant Nos.62105278 and 11674273)the Natural Science Foundation of Shandong Province(Grant No.ZR2023MA015)。
文摘A method for correlating thermal light over a wide spectral range is proposed.A multi-wavelength pseudothermal source,prepared by projecting laser beams of multiple wavelengths(650 nm,635 nm,532 nm,and 473 nm)onto a moving thin ground glass plate,is employed in a double-slit interference experiment.The ground glass plate induces random phase differences between light beams of different wavelengths passing through it.This initial random phase difference significantly influences the high-order intensity correlation functions of multi-wavelength thermal beams.Experimentally,second-order correlated interference patterns,including subwavelength interference,of pseudothermal beams with different wavelengths are observed in the intensity correlation measurements.This method facilitates applications of correlated thermal photons in quantum information processing and quantum imaging.
基金financially supported by the Natural Science Foundation of Shandong Province(No.ZR2024QE446)。
文摘With the rapid development of intelligent electronic and military equipment,multifunctional flexible materials that integrat electromagnetic interference(EMI)shielding,temperature sensing,and information encryption are urgently required.This study presents a bio-inspired hierarchical composite foam fabricated using supercritical nitrogen foaming technology.This material exhibits a honeycomb structure,with pore cell sizes controllable within a range of 30–92μm by regulating the filler.The carbon fiber felt(CFf)provides efficient reflection of electromagnetic waves,while the chloroprene rubber/carbon fiber/carbon black foam facilitates both wave absorption and temperature monitoring through its optimized conductive network.This synergistic mechanism results in an EMI shielding effectiveness(SE)of 60.06 d B with excellent temperature sensing performance(The temperature coefficient of resistance(TCR)is-2.642%/℃)in the 24–70℃ range.Notably,the material has a thermal conductivity of up to 0.159 W/(m·K),and the bio-inspired layered design enables information encryption,demonstrating the material's potential for secure communication applications.The foam also has tensile properties of up to 5.13 MPa and a tear strength of 33.02 N/mm.This biomimetic design overcomes the traditional limitations of flexible materials and provides a transformative solution for next-generation applications such as flexible electronics,aerospace systems and military equipment,which urgently need integrated electromagnetic protection,thermal management and information security.
基金supported by the National Natural Science Foundation of China(Grant No.12304379)the Natural Science Foundation of Liaoning Province(Grant No.2024BS-269)the Guangdong Basic and Applied Basic Research Foundation(Grant No.025A1515011117)。
文摘High-precision optical frequency measurement serves as a cornerstone of modern science and technology,enabling advancements in fields ranging from fundamental physics to quantum information technologies.Obtaining precise photon frequencies,especially in the ultraviolet or even extreme ultraviolet regimes,is a key goal in both light–matter interaction experiments and engineering applications.High-order harmonic generation(HHG)is an ideal light source for producing such photons.In this work,we propose an optical temporal interference model(OTIM)that establishes an analogy with multi-slit Fraunhofer diffraction(MSFD)to manipulate fine-frequency photon generation by exploiting the temporal coherence of HHG processes.Our model provides a unified physical framework for three distinct non-integer HHG generation schemes:single-pulse,shaped-pulse,and laser pulse train approaches,which correspond to single-MSFD-like,double-MSFD-like,and multi-MSFD-like processes,respectively.Arbitrary non-integer HHG photons can be obtained using our scheme.Our approach provides a new perspective for accurately measuring and controlling photon frequencies in fields such as frequency comb technology,interferometry,and atomic clocks.
基金Science and Technology Planning Project of Guangdong Province,2023B1212060048,Xiande Xie。
文摘Recovered samples of Jilin H5 chondrite experimentally shocked to 12-133 GPa were studied to explore the behavior of opaque minerals under shock loading using SEM-EDS,Raman spectroscopy,and TIMA.The following results were obtained.Firstly,at pressures lower than 53GPa,the opaque minerals still keep the unmelted state,while at 78 GPa and higher,FeNi metal and troilite form eutectic intergrowths occurring as disorderly fine veinlets filling the shock-induced fractures in silicate minerals.Secondly,single kamacite grains still maintain their contour at 12 GPa,but a part of brittle troilite grains was fragmented and squeezed into the shock-induced fractures within kamacite grains.At53 and 133 GPa,many more troilite fragments are poured in the kamacite interior to form disordered hybrid aggregates or to form squiggly strips,respectively.Similar phenomena are observed within single troilite grains,but the mineral squeezed into troilite grains is kamacite.Thirdly,chromite is a hard and refractory oxide mineral.When the shock pressure rises step by step from 12 to 133 GPa,the shock effect of chromite is only fragmentation.Its grain size decreases from tens of um at 53 GPa to a few um at 133 GPa.And,fourthly,native copper exhibits distinct redistribution behavior at high temperature.In Jilin samples shock-loaded to 12 GPa,copper initially located at troilite-kamacite interfaces partially transferred into small troilite grains containing fine FeNi particles.At 53 and 133 GPa,native copper preferentially transferred into larger troilite grains containing more particles of eutectic FeNi metal.
基金supported by the Defitech Foundation(Morges,CH)to FCHthe Bertarelli Foundation-Catalyst program(Gstaad,CH)to FCH+2 种基金the Wyss Center for Bio and Neuroengineering the Lighthouse Partnership for AI-guided Neuromodulation to FCHthe Fonds de recherche du Quebec-Sante(FRQS#342969)to CEPthe Neuro X Postdoctoral Fellowship Program to CEP。
文摘Brain lesions,such as those caused by stroke or traumatic brain injury(TBI),frequently result in persistent motor and cognitive impairments that significantly affect the individual patient's quality of life.Despite differences in the mechanisms of injury,both conditions share a high prevalence of motor and cognitive impairments.These deficits show only limited natural recovery.
