At present, electrical engineering is closely related to our life and provides us with convenient life and services. However, at present, there are still some problems and interference factors in electrical engineerin...At present, electrical engineering is closely related to our life and provides us with convenient life and services. However, at present, there are still some problems and interference factors in electrical engineering automation equipment, which affect the performance of electrical engineering automation equipment. Therefore, in view of these interference problems, relevant departments should take effective measures to solve these problems, do a good job in anti-interference measures, constantly promote its use efficiency, and provide better services for our lives.展开更多
DNAzyme machines play critical roles in the fields of cell imaging,disease diagnosis,and cancer therapy.However,the applications of DNAzyme machines are limited by the nucleases-induced degradation,non-specific bindin...DNAzyme machines play critical roles in the fields of cell imaging,disease diagnosis,and cancer therapy.However,the applications of DNAzyme machines are limited by the nucleases-induced degradation,non-specific binding of proteins,and insufficient provision of cofactors.Herein,protected DNAzyme machines with different cofactor designs(referred to as Pro Ds)were nanoengineered by the construction of multifunctional metal-phenolic nanoshells to deactivate the interferential proteins,including nucleases and non-specific binding proteins.Moreover,the nanoshells not only facilitate the cellular internalization of Pro Ds but provide specific metal ions acting as cofactors of the designed DNAzymes.Cellular imaging results demonstrated that Pro Ds could effectively and simultaneously monitor multiple tumor-related micro RNAs in living cells.This facile and rapid strategy that encapsulates DNAzyme machines into the protective metal-phenolic nanoshells is anticipated to extend to a wide range of functional nucleic acidsbased biomedical applications.展开更多
文摘At present, electrical engineering is closely related to our life and provides us with convenient life and services. However, at present, there are still some problems and interference factors in electrical engineering automation equipment, which affect the performance of electrical engineering automation equipment. Therefore, in view of these interference problems, relevant departments should take effective measures to solve these problems, do a good job in anti-interference measures, constantly promote its use efficiency, and provide better services for our lives.
基金supported by National Talents Program,Double First Class University Plan of Sichuan University,State Key Laboratory of Polymer Materials Engineering(No.sklpme 2020-0301)Natural Science Foundation of Sichuan Province(Nos.2022NSFSC1735,2023NSFSC1097)+5 种基金Fundamental Research Funds for the Central Universities(No.ZYN2022094)National Natural Science Foundation of China(Nos.22178233,22208228)China Postdoctoral Science Foundation(No.2020TQ0209)Fundamental Research Funds for the Central Universities(No.YJ201959)Science and Technology Support Program of Sichuan Province(No.2021YJ0414)Project of Chengdu Science and Technology Bureau(No.2021YF05-02110-SN)。
文摘DNAzyme machines play critical roles in the fields of cell imaging,disease diagnosis,and cancer therapy.However,the applications of DNAzyme machines are limited by the nucleases-induced degradation,non-specific binding of proteins,and insufficient provision of cofactors.Herein,protected DNAzyme machines with different cofactor designs(referred to as Pro Ds)were nanoengineered by the construction of multifunctional metal-phenolic nanoshells to deactivate the interferential proteins,including nucleases and non-specific binding proteins.Moreover,the nanoshells not only facilitate the cellular internalization of Pro Ds but provide specific metal ions acting as cofactors of the designed DNAzymes.Cellular imaging results demonstrated that Pro Ds could effectively and simultaneously monitor multiple tumor-related micro RNAs in living cells.This facile and rapid strategy that encapsulates DNAzyme machines into the protective metal-phenolic nanoshells is anticipated to extend to a wide range of functional nucleic acidsbased biomedical applications.
