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金针菇Fvpal1基因的RNAi降低PAL酶活及菌丝色素分泌 被引量:1
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作者 陆欢 沈玲 +4 位作者 刘建雨 尚晓冬 王瑞娟 谭琦 唐桂容 《菌物学报》 北大核心 2025年第4期81-97,共17页
本研究构建了金针菇Flammulina filiformis苯丙氨酸解氨酶基因1(F.filiformis PAL gene1,Fvpal1)的RNAi载体,以黄色的单核体菌株0990-(5)为受体,通过遗传转化获得5个基因沉默的单核转化子(RNAi-Fvpal11–5)。5个单核转化子再分别与白色... 本研究构建了金针菇Flammulina filiformis苯丙氨酸解氨酶基因1(F.filiformis PAL gene1,Fvpal1)的RNAi载体,以黄色的单核体菌株0990-(5)为受体,通过遗传转化获得5个基因沉默的单核转化子(RNAi-Fvpal11–5)。5个单核转化子再分别与白色的单核体菌株Dan3进行杂交,获得5个基因沉默的双核转化子(ZRNAi-Fvpal11–5)。考察并分析了单核转化子和双核转化子在PDA培养基上的菌丝生长速度、菌丝的PAL酶活、菌丝在培养基上的色素分泌及Fvpal1基因表达量情况,以验证Fvpal1基因具有调控金针菇颜色的功能。结果显示,10个转化子的Fvpal1基因表达较野生菌株相比都显著下调(P<0.05),其中转化子RNAi-Fvpal11–5分别下调83.41%、75.92%、79.69%、66.49%和43.22%,转化子ZRNAi-Fvpal11–5分别下调80.26%、45.24%、34.09%、84.05%和79.62%;除转化子ZRNAi-Fvpal14外的9个转化子的PAL酶活力都显著低于出发菌株(P<0.05)。10个转化子的菌丝在PDA培养基上的色素分泌都比出发菌株浅,双核转化子的菌丝在木屑培养基中的颜色也显著变浅,以及双核转化子的子实体颜色也比出发菌株浅。本研究构建了金针菇Fvpal1基因的RNAi体系,发现该基因对金针菇菌丝和子实体的颜色具有正调控作用,为进一步开展金针菇Fvpal1基因的功能基因研究提供了数据支撑。 展开更多
关键词 金针菇 Fvpal1基因 RNA干扰 颜色
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棉铃虫原肌球蛋白基因HaTm-1的RNAi效应及功能分析
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作者 关若冰 韩锐 +3 位作者 楚冬冬 徐赛博 刘晓光 李祥 《昆虫学报》 北大核心 2025年第9期1193-1202,共10页
【目的】本研究旨在对前期转录组测序鉴定到的棉铃虫Helicoverpa armigera原肌球蛋白基因HaTm-1进行组织表达谱和RNAi效应分析,探究HaTm-1作为鳞翅目(Lepidoptera)害虫广谱性RNA生物农药靶点的可行性和理论依据。【方法】PCR克隆棉铃虫H... 【目的】本研究旨在对前期转录组测序鉴定到的棉铃虫Helicoverpa armigera原肌球蛋白基因HaTm-1进行组织表达谱和RNAi效应分析,探究HaTm-1作为鳞翅目(Lepidoptera)害虫广谱性RNA生物农药靶点的可行性和理论依据。【方法】PCR克隆棉铃虫HaTm-1 cDNA序列并进行生物信息学分析;利用RT-qPCR检测HaTm-1在棉铃虫4龄幼虫不同组织(表皮、脂肪体、中肠、脑、精巢和马氏管)以及2-5龄幼虫表皮中的表达量;通过喷洒dsHaTm-1对棉铃虫和草地贪夜蛾Spodoptera frugiperda 1龄幼虫HaTm-1进行RNAi,分别检测棉铃虫HaTm-1和草地贪夜蛾SfTm-1的表达量以及幼虫体重和幼虫存活率;对棉铃虫4龄幼虫注射dsHaTm-1,检测幼虫体重、蛹重、相对生长速率、相对代谢速率和取食量。【结果】棉铃虫HaTm-1全长cDNA为855 bp (GenBank登录号:XP021201113.1), HaTm-1与多物种中的同源蛋白都有超90%的氨基酸序列一致性。棉铃虫与草地贪夜蛾的Tm-1汇聚在同一分支上,亲缘关系最近。HaTm-1在棉铃虫4龄幼虫表皮中特异性高表达,且在不同龄期幼虫表皮中均有表达,其中在5龄幼虫表皮中表达量最高。与dsEGFP对照组相比,喷洒dsHaTm-1处理棉铃虫1龄幼虫12和24 h时,HaTm-1表达量分别降低了55.73%和50.85%;168 h时幼虫存活率显著降低,仅为43.33%;处理后72, 120和168 h时幼虫体重分别下降17.67%, 14.65%和18.90%。与dsEGFP对照组相比,喷洒dsHaTm-1处理草地贪夜蛾1龄幼虫12和24 h时SfTm-1表达量分别降低了44.72%和39.65%;144 h时幼虫存活率为60%;72和120 h时幼虫体重分别下降了13.00%和28.11%。与dsEGFP对照组相比,注射dsHaTm-1处理棉铃虫4龄幼虫24 h时HaTm-1表达量显著降低了55.66%,处理后72和120 h幼虫体重分别下降了50.34%和54.91%,蛹重也下降了13.14%,相对生长速率下降14.00%,相对代谢速率下降15.66%,取食量下降14.50%。取食消化过程受到影响进而导致了幼虫的死亡。【结论】干扰HaTm-1表达对棉铃虫的取食消化过程至关重要。本研究证明了原肌球蛋白作为潜在RNA生物农药广谱性害虫防治靶标的可行性,为其实际应用提供参考。 展开更多
关键词 棉铃虫 草地贪夜蛾 原肌球蛋白 rnai 存活率 体重 取食量
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GTP环化水解酶——一种防治褐飞虱(Nilaparvata lugens)的RNAi靶标基因 被引量:1
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作者 董代幸 羊桂英 +2 位作者 王爱英 罗举 刘淑华 《浙江农业学报》 北大核心 2025年第6期1285-1292,共8页
磷酸鸟苷环化水解酶I(guanosine triphosphate cyclohydrolase I, GCHI)是全变态昆虫重要的生长发育调节因子,参与黑色素形成和蜕皮过程。为探讨了GCHI编码基因作为褐飞虱RNA干扰(RNA interference, RNAi)防控靶标基因的可行性,克隆了... 磷酸鸟苷环化水解酶I(guanosine triphosphate cyclohydrolase I, GCHI)是全变态昆虫重要的生长发育调节因子,参与黑色素形成和蜕皮过程。为探讨了GCHI编码基因作为褐飞虱RNA干扰(RNA interference, RNAi)防控靶标基因的可行性,克隆了褐飞虱的NlGCHI基因,分析了其时空表达特征,并采用显微注射方式进行了功能验证。结果表明,NlGCHI有2个转录本,分别命名为NlGCHIa和NlGCHIb。其中,NlGCHIb在高龄褐飞虱若虫期存在周期性表达动态,且在表皮中表达水平最高。无论是注射dsNlGCHI还是喂食GCHI抑制剂(3-deoxy-D-arabino-heptulosonate-7-phosphate, DAHP),褐飞虱都有极高的死亡率,主要是死于蜕皮障碍,表现为虫蜕不能顺利脱离虫体。dsNlGCHI处理试虫还表现出不能正常黑化的现象。以上结果表明,GCHI在全变态昆虫和不完全变态昆虫中的功能相对保守,影响昆虫的表皮形成和蜕皮过程,其编码基因可用于开发防治褐飞虱的RNAi农药。 展开更多
关键词 褐飞虱 RNA干扰 磷酸鸟苷环化水解酶Ⅰ 表皮
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RNA interference with foxl 2 leads to upregulation of cyp 19 b and delayed development of female carp Cyprinus carpio var.koi gonads
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作者 Xiao MEI Xinglei WANG +5 位作者 Qiang ZHOU Bo LIU Yishun WANG Liuchang KONG Shibo JIANG Feng WANG 《Journal of Oceanology and Limnology》 2025年第3期954-966,共13页
FOXL 2 and CYP 19 B are crucial transcription factors in vertebrates and invertebrates that play pivotal roles in sex differentiation and gonadal development.The potential roles of the foxl 2 and cyp 19 b genes in sex... FOXL 2 and CYP 19 B are crucial transcription factors in vertebrates and invertebrates that play pivotal roles in sex differentiation and gonadal development.The potential roles of the foxl 2 and cyp 19 b genes in sex determination and gonadal development in Cyprinus carpio var.koi were explored using a non-invasive RNA interference(RNAi)method,histopathological observation and qPCR.Results demonstrate that foxl 2 exhibited a sexually dimorphic expression pattern in gonads,with a notable expression in ovaries;cyp 19 b was expressed in all peripheral tissues,with a particularly prominent expression in brain and gonads.The knockdown of foxl 2 by RNAi resulted in delay in the development of the female gonads.Conversely,no notable alterations were discerned in the gonads of C.carpio var.koi following the knockdown of cyp 19 b.The upregulation of sox 9 a,amh,and cyp 19 b following foxl 2 knockdown indicates that foxl 2 may play a pivotal role in gonadal development.Nevertheless,further investigation is required to ascertain the potential role of cyp 19 b.This study elucidated the role of foxl 2 and enhanced the understanding of the mechanisms of sex determination and gonadal development in C.carpio var.koi. 展开更多
关键词 Cyprinus carpio var.koi RNA interference(rnai) foxl 2 cyp 19 b sex differentiation gonadal development
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Innovative trends in RNA interference drugs:a comprehensive patent analysis
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作者 Xing Li Lu Ma +2 位作者 Yali Zhao Xiaoxia Zhang Zijia Li 《Journal of Chinese Pharmaceutical Sciences》 2025年第8期701-714,共14页
Nucleic acid drugs represent the third wave of innovation in drug research and development,succeeding small-molecule and antibody drugs.These drugs,particularly RNA interference(RNAi)therapies,have become a pivotal fo... Nucleic acid drugs represent the third wave of innovation in drug research and development,succeeding small-molecule and antibody drugs.These drugs,particularly RNA interference(RNAi)therapies,have become a pivotal focus in the pharmaceutical industry.RNAi drugs are extensively utilized in the treatment of chronic and rare diseases due to their exceptional gene-silencing efficiency,manageable side effects,and straightforward synthesis process.This study undertook a thorough analysis of the global landscape of RNAi drug patents,highlighting the latest technological advancements and trends.We meticulously identified and cataloged the key technologies that dominated this patent landscape.The goal was to provide valuable insights and references for researchers involved in the development of RNAi drugs within the domestic pharmaceutical sector. 展开更多
关键词 Nucleic acid drugs RNA interference(rnai)drugs siRNA Patent analysis
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RNAi technologies for insect control in crop protection
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作者 Xue Zhong Xiudao Yu +5 位作者 Jiahui Zhang Jiajing Xu Mengchao Qin Mingxin Cao Frédéric Francis Lanqin Xia 《The Crop Journal》 2025年第4期1009-1021,共13页
RNA interference(RNAi)has been used for agricultural insect pest control based on silencing of targeted insect genes.However,the effectiveness of RNAi and its applications in insect pest control remain challenging.Her... RNA interference(RNAi)has been used for agricultural insect pest control based on silencing of targeted insect genes.However,the effectiveness of RNAi and its applications in insect pest control remain challenging.Here we review factors that may affect the effectiveness of RNAi application,including the variability in RNAi efficacy among different insect species,a limited understanding of double-stranded RNA(dsRNA)uptake and systemic RNAi mechanisms,and the effective delivery of dsRNA in field conditions.Furthermore,we summarize recent progress in RNAi strategies for crop protection,discuss the advantages and disadvantages of RNAi-based insect control,and propose potential strategies to increase the effectiveness of RNAi in insect control. 展开更多
关键词 Crop protection Double-stranded RNA(dsRNA) Insect control RNA interference(rnai)
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Multistage microcellular waterborne polyurethane composite with optionally low-reflection behavior for ultra-efficient electromagnetic interference shielding 被引量:3
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作者 Jianming Yang Hu Wang +4 位作者 Hexin Zhang Peng Lin Hong Gao Youyi Xia Xia Liao 《Journal of Materials Science & Technology》 2025年第5期132-140,共9页
Electromagnetic interference(EMI)shielding materials with superior shielding efficiency and low-reflection properties hold promising potential for utilization across electronic components,precision instruments,and fif... Electromagnetic interference(EMI)shielding materials with superior shielding efficiency and low-reflection properties hold promising potential for utilization across electronic components,precision instruments,and fifth-generation communication equipment.In this study,multistage microcellular waterborne polyurethane(WPU)composites were constructed via gradient induction,layer-by-layer casting,and supercritical carbon dioxide foaming.The gradient-structured WPU/ironcobalt loaded reduced graphene oxide(FeCo@rGO)foam serves as an impedance-matched absorption layer,while the highly conductive WPU/silver loaded glass microspheres(Ag@GM)layer is employed as a reflection layer.Thanks to the incorporation of an asymmetric structure,as well as the introduction of gradient and porous configurations,the composite foam demonstrates excellent conductivity,outstanding EMI SE(74.9 dB),and minimal reflection characteristics(35.28%)in 8.2-12.4 GHz,implying that more than 99.99999%of electromagnetic(EM)waves were blocked and only 35.28%were reflected to the external environment.Interestingly,the reflectivity of the composite foam is reduced to 0.41%at 10.88 GHz due to the resonance for incident and reflected EM waves.Beyond that,the composite foam is characterized by low density(0.47 g/cm^(3))and great stability of EMI shielding properties.This work offers a viable approach for craft-ing lightweight,highly shielding,and minimally reflective EMI shielding composites. 展开更多
关键词 Electromagnetic interference shielding Supercritical carbon dioxide(ScCO_(2))foaming Low reflectivity Multilayered structure MICROCELLULAR
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Learning Based Interference Coordination for Maritime Communications 被引量:1
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作者 Liu Chuhuan Xiao Liang +3 位作者 Chen Yifan Li Siyao Yang Helin Lyu Zefang 《China Communications》 2025年第4期356-374,共19页
With the boom in maritime activities,the need for highly reliable maritime communication is becoming urgent,which is an important component of 5G/6G communication networks.However,the bandwidth reuse characteristic of... With the boom in maritime activities,the need for highly reliable maritime communication is becoming urgent,which is an important component of 5G/6G communication networks.However,the bandwidth reuse characteristic of 5G/6G networks will inevitably lead to severe interference,resulting in degradation in the communication performance of maritime users.In this paper,we propose a safe deep reinforcement learning based interference coordination scheme to jointly optimize the power control and bandwidth allocation in maritime communication systems,and exploit the quality-of-service requirements of users as the risk value references to evaluate the communication policies.In particular,this scheme designs a deep neural network to select the communication policies through the evaluation network and update the parameters using the target network,which improves the communication performance and speeds up the convergence rate.Moreover,the Nash equilibrium of the interference coordination game and the computational complexity of the proposed scheme are analyzed.Simulation and experimental results verify the performance gain of the proposed scheme compared with benchmarks. 展开更多
关键词 bandwidth allocation interference coordination maritime communication power control rein-forcement learning
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Hierarchically Porous Polypyrrole Foams Contained Ordered Polypyrrole Nanowire Arrays for Multifunctional Electromagnetic Interference Shielding and Dynamic Infrared Stealth 被引量:1
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作者 Yu-long Liu Ting-yu Zhu +5 位作者 Qin Wang Zi-jie Huang De-xiang Sun Jing-hui Yang Xiao-dong Qi Yong Wang 《Nano-Micro Letters》 2025年第4期399-418,共20页
As modern communication and detection technologies advance at a swift pace,multifunctional electromagnetic interference(EMI)shielding materials with active/positive infrared stealth,hydrophobicity,and electric-thermal... As modern communication and detection technologies advance at a swift pace,multifunctional electromagnetic interference(EMI)shielding materials with active/positive infrared stealth,hydrophobicity,and electric-thermal conversion ability have received extensive attention.Meeting the aforesaid requirements simultaneously remains a huge challenge.In this research,the melamine foam(MF)/polypyrrole(PPy)nanowire arrays(MF@PPy)were fabricated via one-step electrochemical polymerization.The hierarchical MF@PPy foam was composed of three-dimensional PPy micro-skeleton and ordered PPy nanowire arrays.Due to the upwardly grown PPy nanowire arrays,the MF@PPy foam possessed good hydrophobicity ability with a water contact angle of 142.00°and outstanding stability under various harsh environments.Meanwhile,the MF@PPy foam showed excellent thermal insulation property on account of the low thermal conductivity and elongated ligament characteristic of PPy nanowire arrays.Furthermore,taking advantage of the high conductivity(128.2 S m^(-1)),the MF@PPy foam exhibited rapid Joule heating under 3 V,resulting in dynamic infrared stealth and thermal camouflage effects.More importantly,the MF@PPy foam exhibited remarkable EMI shielding effectiveness values of 55.77 dB and 19,928.57 dB cm^(2)g^(-1).Strong EMI shielding was put down to the hierarchically porous PPy structure,which offered outstanding impedance matching,conduction loss,and multiple attenuations.This innovative approach provides significant insights to the development of advanced multifunctional EMI shielding foams by constructing PPy nanowire arrays,showing great applications in both military and civilian fields. 展开更多
关键词 Polypyrrole nanowire arrays Hierarchical foam HYDROPHOBICITY Infrared stealth Electromagnetic interference shielding
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Multifunctional Nacre‑Like Nanocomposite Papers for Electromagnetic Interference Shielding via Heterocyclic Aramid/MXene Template‑Assisted In‑Situ Polypyrrole Assembly
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作者 Jinhua Xiong Xu Zhao +6 位作者 Zonglin Liu He Chen Qian Yan Huanxin Lian Yunxiang Chen Qingyu Peng Xiaodong He 《Nano-Micro Letters》 SCIE EI CAS 2025年第3期37-54,共18页
Robust, ultra-flexible, and multifunctional MXene-basedelectromagnetic interference (EMI) shielding nanocomposite filmsexhibit enormous potential for applications in artificial intelligence,wireless telecommunication,... Robust, ultra-flexible, and multifunctional MXene-basedelectromagnetic interference (EMI) shielding nanocomposite filmsexhibit enormous potential for applications in artificial intelligence,wireless telecommunication, and portable/wearable electronic equipment.In this work, a nacre-inspired multifunctional heterocyclic aramid(HA)/MXene@polypyrrole (PPy) (HMP) nanocomposite paper withlarge-scale, high strength, super toughness, and excellent tolerance tocomplex conditions is fabricated through the strategy of HA/MXenehydrogel template-assisted in-situ assembly of PPy. Benefiting from the"brick-and-mortar" layered structure and the strong hydrogen-bondinginteractions among MXene, HA, and PPy, the paper exhibits remarkable mechanical performances, including high tensile strength (309.7 MPa),outstanding toughness (57.6 MJ m−3), exceptional foldability, and structural stability against ultrasonication. By using the template effect ofHA/MXene to guide the assembly of conductive polymers, the synthesized paper obtains excellent electronic conductivity. More importantly,the highly continuous conductive path enables the nanocomposite paper to achieve a splendid EMI shielding effectiveness (EMI SE) of 54.1 dBat an ultra-thin thickness (25.4 μm) and a high specific EMI SE of 17,204.7 dB cm2g−1. In addition, the papers also have excellent applicationsin electromagnetic protection, electro-/photothermal de-icing, thermal therapy, and fire safety. These findings broaden the ideas for developinghigh-performance and multifunctional MXene-based films with enormous application potential in EMI shielding and thermal management. 展开更多
关键词 MXene Remarkable mechanical properties Heterocyclic aramid Electromagnetic interference shielding POLYPYRROLE Multifunctionality
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Improved algorithm of multi-mainlobe interference suppression under uncorrelated and coherent conditions 被引量:1
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作者 CAI Miaohong CHENG Qiang +1 位作者 MENG Jinli ZHAO Dehua 《Journal of Southeast University(English Edition)》 2025年第1期84-90,共7页
A new method based on the iterative adaptive algorithm(IAA)and blocking matrix preprocessing(BMP)is proposed to study the suppression of multi-mainlobe interference.The algorithm is applied to precisely estimate the s... A new method based on the iterative adaptive algorithm(IAA)and blocking matrix preprocessing(BMP)is proposed to study the suppression of multi-mainlobe interference.The algorithm is applied to precisely estimate the spatial spectrum and the directions of arrival(DOA)of interferences to overcome the drawbacks associated with conventional adaptive beamforming(ABF)methods.The mainlobe interferences are identified by calculating the correlation coefficients between direction steering vectors(SVs)and rejected by the BMP pretreatment.Then,IAA is subsequently employed to reconstruct a sidelobe interference-plus-noise covariance matrix for the preferable ABF and residual interference suppression.Simulation results demonstrate the excellence of the proposed method over normal methods based on BMP and eigen-projection matrix perprocessing(EMP)under both uncorrelated and coherent circumstances. 展开更多
关键词 mainlobe interference suppression adaptive beamforming spatial spectral estimation iterative adaptive algorithm blocking matrix preprocessing
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Inter‑Skeleton Conductive Routes Tuning Multifunctional Conductive Foam for Electromagnetic Interference Shielding,Sensing and Thermal Management
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作者 Xufeng Li Chunyan Chen +10 位作者 Zhenyang Li Peng Yi Haihan Zou Gao Deng Ming Fang Junzhe He Xin Sun Ronghai Yu Jianglan Shui Caofeng Pan Xiaofang Liu 《Nano-Micro Letters》 SCIE EI CAS 2025年第3期19-36,共18页
Conductive polymer foam(CPF)with excellent compressibility and variable resistance has promising applications in electromagnetic interference(EMI)shielding and other integrated functions for wearable electronics.Howev... Conductive polymer foam(CPF)with excellent compressibility and variable resistance has promising applications in electromagnetic interference(EMI)shielding and other integrated functions for wearable electronics.However,its insufficient change amplitude of resistance with compressive strain generally leads to a degradation of shielding performance during deformation.Here,an innovative loading strategy of conductive materials on polymer foam is proposed to significantly increase the contact probability and contact area of conductive components under compression.Unique inter-skeleton conductive films are constructed by loading alginate-decorated magnetic liquid metal on the polymethacrylate films hanged between the foam skeleton(denoted as AMLM-PM foam).Traditional point contact between conductive skeletons under compression is upgraded to planar contact between conductive films.Therefore,the resistance change of AMLM-PM reaches four orders of magnitude under compression.Moreover,the inter-skeleton conductive films can improve the mechanical strength of foam,prevent the leakage of liquid metal and increase the scattering area of EM wave.AMLM-PM foam has strain-adaptive EMI shielding performance and shows compression-enhanced shielding effectiveness,solving the problem of traditional CPFs upon compression.The upgrade of resistance response also enables foam to achieve sensitive pressure sensing over a wide pressure range and compression-regulated Joule heating function. 展开更多
关键词 Inter-skeleton conductive films Conductive polymer foam Liquid metal Electromagnetic interference shielding
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Overview of mosquito control based on RNA interference
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作者 Shi-Wen Duan Gang Liu Yin-Liang Wang 《Life Research》 2025年第3期74-77,共4页
RNA interference(RNAi)is a post-transcriptional gene-silencing technique induced by the introduction of double-stranded RNA(dsRNA)or small interfering RNA(siRNA)[1].RNAi-based strategies have been extensively applied ... RNA interference(RNAi)is a post-transcriptional gene-silencing technique induced by the introduction of double-stranded RNA(dsRNA)or small interfering RNA(siRNA)[1].RNAi-based strategies have been extensively applied in the treatment of human diseases and crop protection against insect pests[2-4].With the availability of the full genome sequences of major mosquito vectors,RNAi has become increasingly used as a novel means of mosquito control[5]. 展开更多
关键词 mosquito control DSRNA treatment human diseases RNA interference insect pests small interfering rna sirna rnai based crop protection rna interference rnai
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Improving DOA estimation of GNSS interference through sparse non-uniform array reconfiguration 被引量:1
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作者 Rongling LANG Hao XU +3 位作者 Fei GAO Zewen TANG Zhipeng WANG Amir HUSSAIN 《Chinese Journal of Aeronautics》 2025年第8期104-118,共15页
Interference significantly impacts the performance of the Global Navigation Satellite Systems(GNSS),highlighting the need for advanced interference localization technology to bolster anti-interference and defense capa... Interference significantly impacts the performance of the Global Navigation Satellite Systems(GNSS),highlighting the need for advanced interference localization technology to bolster anti-interference and defense capabilities.The Uniform Circular Array(UCA)enables concurrent estimation of the Direction of Arrival(DOA)in both azimuth and elevation.Given the paramount importance of stability and real-time performance in interference localization,this work proposes an innovative approach to reduce the complexity and increase the robustness of the DOA estimation.The proposed method reduces computational complexity by selecting a reduced number of array elements to reconstruct a non-uniform sparse array from a UCA.To ensure DOA estimation accuracy,minimizing the Cramér-Rao Bound(CRB)is the objective,and the Spatial Correlation Coefficient(SCC)is incorporated as a constraint to mitigate side-lobe.The optimization model is a quadratic fractional model,which is solved by Semi-Definite Relaxation(SDR).When the array has perturbations,the mathematical expressions for CRB and SCC are re-derived to enhance the robustness of the reconstructed array.Simulation and hardware experiments validate the effectiveness of the proposed method in estimating interference DOA,showing high robustness and reductions in hardware and computational costs associated with DOA estimation. 展开更多
关键词 GNSS interference location Direction of arrival estimation Adaptive reconfigurable array Cramér-Raobound Quadratic fractional programming
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小菜蛾PxylPBAN的克隆、原核表达及其RNAi对保幼激素和性信息素通路基因表达的影响
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作者 郑月琴 祝潇雯 +3 位作者 田厚军 林硕 刘倩霞 魏辉 《昆虫学报》 北大核心 2025年第5期576-584,共9页
【目的】通过对小菜蛾Plutella xylostella性信息素生物合成激活神经肽基因PxylPBAN进行克隆、原核表达,并检测RNAi沉默PxylPBAN对小菜蛾保幼激素通路和性信息素合成通路基因表达的影响,探讨PxylPBAN在性信息素合成中的作用。【方法】利... 【目的】通过对小菜蛾Plutella xylostella性信息素生物合成激活神经肽基因PxylPBAN进行克隆、原核表达,并检测RNAi沉默PxylPBAN对小菜蛾保幼激素通路和性信息素合成通路基因表达的影响,探讨PxylPBAN在性信息素合成中的作用。【方法】利用RT-PCR克隆小菜蛾PxylPBAN的CDS全长序列;原核表达PxylPBAN并纯化;通过将dsPxylPBAN注射进小菜蛾雌蛹,利用RNAi沉默PxylPBAN,并利用RT-qPCR验证RNAi后24, 48和72 h时PxylPBAN以及保幼激素通路基因(PxylJHAMT,PxylnJHBP,PxylhJHBP和PxylMet)和性信息素合成通路基因(PxylACC和PxylFAR6)的表达量。【结果】克隆得到小菜蛾PxylPBAN(GenBank登录号:LOC105391112),全长CDS 582 bp,编码193个氨基酸,蛋白预测分子量约为21.85 kD;原核表达获得PxylPBAN重组蛋白。RNAi结果表明与注射dsEGFP的对照比较,注射dsPxylPBAN后小菜蛾PxylPBAN的表达量显著下调,且注射后24 h时下调最显著;此外,PxylJHAMT,PxylnJHBP,PxylhJHBP,PxylMet,PxylACC和PxylFAR6的表达量也显著下调。【结论】本研究成功获得PxylPBAN重组蛋白,发现PxylPBAN是小菜蛾保幼激素通路和性信息素合成通路的重要枢纽基因,为揭示小菜蛾PxylPBAN与保幼激素对性信息素生物合成的联动调控模式奠定理论基础。 展开更多
关键词 小菜蛾 性信息素生物合成激活神经肽 保幼激素 基因克隆 原核表达 RNA干扰
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基于RNAi育种的农作物病虫害联合控制研究进展
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作者 郭芮 曹晓伟 郭伟 《中国农学通报》 2025年第21期10-17,共8页
为了构筑高效、可持续的RNAi育种防控体系,本综述归纳了RNAi技术在作物抗病虫中的应用潜力,总结了当前转基因RNAi作物的研发现状,分析了多靶点串联RNAi的设计策略与协同抗性机制;重点阐述了“dsRNA/microRNA稳定表达系统”“复合病虫害... 为了构筑高效、可持续的RNAi育种防控体系,本综述归纳了RNAi技术在作物抗病虫中的应用潜力,总结了当前转基因RNAi作物的研发现状,分析了多靶点串联RNAi的设计策略与协同抗性机制;重点阐述了“dsRNA/microRNA稳定表达系统”“复合病虫害(小麦赤霉病-蚜虫、棉花黄萎病-棉蚜)联合控制模型”“延缓抗性进化的基因精准干预途径”等内容,指出递送效率低、dsRNA环境稳定性差及生产成本高仍是RNAi技术规模化应用的瓶颈。提出通过纳米载体-植物共生递送、串联表达多基因沉默盒、结合生态平衡监测的精准施放技术,有望在5~10年内实现多病虫害协同治理;认为该体系将推动农业向“基因精准干预+生态平衡维护”的可持续模式转型,为全球粮食安全提供关键支撑。 展开更多
关键词 病虫害 rnai育种 基因沉默 串联表达 多重抗性
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RNAi技术在植物寄生线虫防控中的应用研究进展
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作者 于敬文 黄凌 +5 位作者 赵俊丽 仝允正 刘雅琴 陈燕雪 陈新娟 黄文坤 《湖南农业科学》 2025年第5期79-84,共6页
植物寄生线虫寄主范围广,严重危害作物根系,防治十分困难。RNAi技术能够使植物寄生线虫关键靶标基因的表达下调,具有特异性强、安全性高等优点。在植物与线虫互作过程中,RNAi技术已广泛应用于线虫的特定基因功能及防控技术研究。本文从R... 植物寄生线虫寄主范围广,严重危害作物根系,防治十分困难。RNAi技术能够使植物寄生线虫关键靶标基因的表达下调,具有特异性强、安全性高等优点。在植物与线虫互作过程中,RNAi技术已广泛应用于线虫的特定基因功能及防控技术研究。本文从RNAi技术的起源及作用机制、植物寄生线虫RNAi靶基因的选择以及dsRNA防控植物寄生线虫的递送策略几个方面进行综述,并对RNAi在植物寄生线虫防控中的应用前景进行探讨,以期为植物寄生线虫病害的防控提供新思路。 展开更多
关键词 RNA干扰 植物寄生线虫 dsRNA递送策略 线虫与植物互作 综述
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简析OECD《喷洒或外源施用双链RNAi农药的环境风险评估要点》
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作者 简秋 宗伏霖 《农药科学与管理》 2025年第9期1-3,17,共4页
本文重点介绍了经济合作与发展组织(OECD)《喷洒或外源施用双链RNAi农药的环境风险评估要点》(农药系列报告第104号)中有关环境风险识别,以及风险评估中需特别考虑的关注点和风险评估建议。提出了进一步加强研究,多方合作,建立相应的标... 本文重点介绍了经济合作与发展组织(OECD)《喷洒或外源施用双链RNAi农药的环境风险评估要点》(农药系列报告第104号)中有关环境风险识别,以及风险评估中需特别考虑的关注点和风险评估建议。提出了进一步加强研究,多方合作,建立相应的标准化测试和评价方法,以支持这一新兴农药类别的科学监管的建议。 展开更多
关键词 rnai农药 环境风险评估
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Analysis of auxiliary antenna array effect on performance of wideband noncooperative interference cancellation
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作者 LI Zheyu LI Yaxing +3 位作者 WANG Ze LIANG Jiaqi HE Fangmin MENG Jin 《Journal of Systems Engineering and Electronics》 2025年第5期1188-1201,共14页
In wideband noncooperative interference cancellation,the reference signals obtained through auxiliary antennas are weighted to cancel with the interference signal.The correlation between the reference signal and the i... In wideband noncooperative interference cancellation,the reference signals obtained through auxiliary antennas are weighted to cancel with the interference signal.The correlation between the reference signal and the interference signal determines interference cancellation performance,while the auxiliary antenna array affects the correlation by influencing the amplitude and phase of the reference signals.This paper analyzes the effect of auxiliary antenna array on multiple performances of wideband noncooperative interference cancellation.Firstly,the array received signal model of wideband interference is established,and the weight vector coupled with the auxiliary antennas array manifold is solved by spectral analysis and eigen-subspace decomposition.Then,multiple performances which include cancellation resolution,grating null,wideband interference cancellation ratio(ICR),and convergence rate are quantitatively characterized with the auxiliary antenna array.It is obtained through analysis that the performances mutually restrict the auxiliary antenna array.Higher cancellation resolution requires larger array aperture,but when the number of auxiliary antennas is fixed,larger array aperture results in more grating nulls.When the auxiliary antennas are closer to the main antenna,the wideband ICR is improved,but the convergence rate is reduced.The conclusions are verified through simulation of one-dimensional uniform array and two-dimensional nonuniform array.The experiments of three arrays are compared,and the results conform well with simulation and support the theoretical analysis. 展开更多
关键词 auxiliary antenna array noncooperative interference cancellation wideband interference performance analysis
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Anti-interference methods for narrowband and repeater-modulation interference in airborne SAR systems
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作者 Kai Chen Yong-Bo Zhao +3 位作者 Kai Jiang Long Sun Kun Deng Chang-Chun Ding 《Journal of Electronic Science and Technology》 2025年第3期1-12,共12页
With the advancement of electronic countermeasures,airborne synthetic aperture radar(SAR)systems are facing increasing challenges in maintaining effective performance in hostile environments.In particular,high-power i... With the advancement of electronic countermeasures,airborne synthetic aperture radar(SAR)systems are facing increasing challenges in maintaining effective performance in hostile environments.In particular,high-power interference can severely degrade SAR imaging and signal processing,often rendering target detection impossible.This highlights the urgent need for robust anti-interference solutions in both the signal processing and image processing domains.While current methods address interference across various domains,techniques such as waveform modification and spatial filtering typically increase the system costs and complexity.To overcome these limitations,we propose a novel approach that leverages the multi-domain characteristics of interference to efficiently suppress narrowband interference and repeater modulation interference.Specifically,narrowband interference is mitigated using notch filtering,a signal processing technique that effectively filters out unwanted frequencies,while repeater modulation interference is addressed through strong signal amplitude normalization,which enhances both the signal and image processing quality.These methods were validated through tests on real SAR data,demonstrating significant improvements in the imaging performance and system robustness.Our approach offers valuable insights for advancing anti-interference technologies in SAR systems and provides a cost-effective solution to enhance their resilience in complex electronic warfare environments. 展开更多
关键词 Airborne radar Narrowband interference Repeater modulation interference Signal and image processing ANTI-interference
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