Considering the CRTS-II track slab,which is commonly used in the Chinese high-speed railway system,a vehicle-track-bridge dynamic analysis method is proposed in which the vehicle subsystem equations are established by...Considering the CRTS-II track slab,which is commonly used in the Chinese high-speed railway system,a vehicle-track-bridge dynamic analysis method is proposed in which the vehicle subsystem equations are established by the rigid body dynamics method,the track subsystem and the bridge subsystem equations are established by the FEM,the wheel-rail contact relation is defined by the corresponding assumption in vertical direction and the Kalker linear creep theory in lateral direction.The in-span spring element is derived to model the track-bridge interaction;the equal-band-width storage is adopted to fit the track structure with multilayer uniform section beam;and the dynamic equilibrium equations are solved by the inter-history iteration method.As a case study,the response of a CRH2 high-speed train transverses a simply-supported bridge with successive 31.5m double bound pre-stress beams is simulated.The result shows that using the vehicle-track-bridge interaction model instead of the vehicle-bridge interaction model helps predict the rotation angle at beam ends and choose an economic beam vertical stiffness.展开更多
In order to resolve the multisensor multiplied maneuvering target tracking problem, this paper presents a distributed interacted multiple model multisensor joint probabilistic data association algorithm （DIMM-MSJPDA...In order to resolve the multisensor multiplied maneuvering target tracking problem, this paper presents a distributed interacted multiple model multisensor joint probabilistic data association algorithm （DIMM-MSJPDA）. First of all, the interacted multiple model joint probabilistic data association algorithm is applied to each sensor, and then the state estimation, estimation covariance, model probability, combined innovation, innovation covariance are delivered to the fusion center. Then, the tracks from each sensor are correlated and the D-S evidence theory is used to gain the model probability of an identical target. Finally, the ultimate state estimation of each target is calculated according to the new model probability, and the state estimation is transmitted to each sensor. Simulations are designed to test the tracking performance of DIMM-MSJPDA algorithm. The results show that the use of DIMM-MSJPDA algorithm enables the distributed multisensor system to track multiplied maneuvering targets and its tracking performance is much better than that of IMMJPDA algorithm.展开更多
The nervous system processes a vast amount of information,performing computations that underlie perception,cognition,and behavior.During development,neuronal guidance genes,which encode extracellular cues,their recept...The nervous system processes a vast amount of information,performing computations that underlie perception,cognition,and behavior.During development,neuronal guidance genes,which encode extracellular cues,their receptors,and downstream signal transducers,organize neural wiring to generate the complex architecture of the nervous system.It is now evident that many of these neuroguidance cues and their receptors are active during development and are also expressed in the adult nervous system.This suggests that neuronal guidance pathways are critical not only for neural wiring but also for ongoing function and maintenance of the mature nervous system.Supporting this view,these pathways continue to regulate synaptic connectivity,plasticity,and remodeling,and overall brain homeostasis throughout adulthood.Genetic and transcriptomic analyses have further revealed many neuronal guidance genes to be associated with a wide range of neurodegenerative and neuropsychiatric disorders.Although the precise mechanisms by which aberrant neuronal guidance signaling drives the pathogenesis of these diseases remain to be clarified,emerging evidence points to several common themes,including dysfunction in neurons,microglia,astrocytes,and endothelial cells,along with dysregulation of neuron-microglia-astrocyte,neuroimmune,and neurovascular interactions.In this review,we explore recent advances in understanding the molecular and cellular mechanisms by which aberrant neuronal guidance signaling contributes to disease pathogenesis through altered cell-cell interactions.For instance,recent studies have unveiled two distinct semaphorin-plexin signaling pathways that affect microglial activation and neuroinflammation.We discuss the challenges ahead,along with the therapeutic potentials of targeting neuronal guidance pathways for treating neurodegenerative diseases.Particular focus is placed on how neuronal guidance mechanisms control neuron-glia and neuroimmune interactions and modulate microglial function under physiological and pathological conditions.Specifically,we examine the crosstalk between neuronal guidance signaling and TREM2,a master regulator of microglial function,in the context of pathogenic protein aggregates.It is well-established that age is a major risk factor for neurodegeneration.Future research should address how aging and neuronal guidance signaling interact to influence an individual’s susceptibility to various late-onset neurological diseases and how the progression of these diseases could be therapeutically blocked by targeting neuronal guidance pathways.展开更多
Background: The human gut microbiome is an important target for disease treatment and prevention. Various microbial species within the complex ecosystem of the microbiome have been shown to play important roles in dis...Background: The human gut microbiome is an important target for disease treatment and prevention. Various microbial species within the complex ecosystem of the microbiome have been shown to play important roles in disease. Identification of bioactive materials capable of altering the abundances of these species both safely and effectively is a major goal in microbiome research. Many traditional Chinese medicines (TCMs) have been reported to affect the composition of the gut microbiome. Here, we summarize studies that have used TCMs to alter the gut microbiome and discuss the response relationship between TCMs and gut microbial species. Methods: We searched the PubMed, Web of Science, and Knowledge Network databases using the terms “traditional Chinese medicine,” “gut microbiome,” and specific system disease names (endocrine, immune, nervous, cardiovascular, and digestive). Studies were excluded if irrelevant or if the experimental procedures were unclear. Results: TCMs have been reported to affect a wide range of gut microbial taxa spanning major phyla, including Firmicutes, Bacteroidetes, Proteobacteria, Verrucomicrobiota, Actinobacteria, and Fusobacteria. In all, 54 TCMs including compounds and extracts have been tested in rodents and 30 have been examined in human trials. Almost all studies have reported positive results in regulating the gut microbiome as well as modulating corresponding phenotypes, spanning diseases of the endocrine, immune, nervous, cardiovascular, and digestive systems. Gut species, including Akkermansia, Bacteroides, Fusobacterium, Faecalibacterium, and E. coli, were found to be regulated by 19 TCMs. A network was constructed to visualize the interactions between TCMs and these taxa. Conclusion: There exists a complex and close relationship between intestinal microflora and diseases. Sufficient experimental data and studies have proved that the imbalance of intestinal microflora affects health by mediating metabolism, immune regulation, inflammation and signal transduction. Many characteristic alterations of intestinal microflora are positively correlated with diseases, so intestinal microflora has become a potential risk index and treatment target for many diseases. Many TCMs affect the relative abundances of microbial species in the gut, and therefore may be useful for modulating the gut microbiome. This review provides a reference for prioritizing candidate TCMs from the enormous repertoire of such medicines to test which specific gut microbes are targeted.展开更多
Studies of wave-current interactions are vital for the safe design of structures.Regular waves in the presence of uniform,linear shear,and quadratic shear currents are explored by the High-Level Green-Naghdi model in ...Studies of wave-current interactions are vital for the safe design of structures.Regular waves in the presence of uniform,linear shear,and quadratic shear currents are explored by the High-Level Green-Naghdi model in this paper.The five-point central difference method is used for spatial discretization,and the fourth-order Adams predictor-corrector scheme is employed for marching in time.The domain-decomposition method is applied for the wave-current generation and absorption.The effects of currents on the wave profile and velocity field are examined under two conditions:the same velocity of currents at the still-water level and the constant flow volume of currents.Wave profiles and velocity fields demonstrate substantial differences in three types of currents owing to the diverse vertical distribution of current velocity and vorticity.Then,loads on small-scale vertical cylinders subjected to regular waves and three types of background currents with the same flow volume are investigated.The maximum load intensity and load fluctuation amplitude in uniform,linear shear,and quadratic shear currents increase sequentially.The stretched superposition method overestimates the maximum load intensity and load fluctuation amplitude in opposing currents and underestimates these values in following currents.The stretched superposition method obtains a poor approximation for strong nonlinear waves,particularly in the case of the opposing quadratic shear current.展开更多
Luminescent metal-organic frameworks(MOFs)have garnered significant attention due to their structural tunability and potential applications in solid-state lighting,bioimaging,sensing,anticounterfeiting,and other field...Luminescent metal-organic frameworks(MOFs)have garnered significant attention due to their structural tunability and potential applications in solid-state lighting,bioimaging,sensing,anticounterfeiting,and other fields.Nevertheless,due to the tendency of1,4-benzenedicarboxylic acid(BDC)to rotate within the framework,MOFs composed of it exhibit significant non-radiative energy dissipation and thus impair the emissive properties.In this study,efficient luminescence of MIL-140A nanocrystals(NCs)with BDC rotors as ligands is achieved by pressure treatment strategy.Pressure treatment effectively modulates the pore structure of the framework,enhancing the interactions between the N,N-dimethylformamide vip molecules and the BDC ligands.The enhanced host-vip interaction contributes to the structural rigidity of the MOF,thereby suppressing the rotation-induced excited-state energy loss.As a result,the pressure-treated MIL-140A NCs displayed bright blue-light emission,with the photoluminescence quantum yield increasing from an initial 6.8%to 69.2%.This study developed an effective strategy to improve the luminescence performance of rotor ligand MOFs,offers a new avenue for the rational design and synthesis of MOFs with superior luminescent properties.展开更多
Reliable traffic flow prediction is crucial for mitigating urban congestion.This paper proposes Attentionbased spatiotemporal Interactive Dynamic Graph Convolutional Network(AIDGCN),a novel architecture integrating In...Reliable traffic flow prediction is crucial for mitigating urban congestion.This paper proposes Attentionbased spatiotemporal Interactive Dynamic Graph Convolutional Network(AIDGCN),a novel architecture integrating Interactive Dynamic Graph Convolution Network(IDGCN)with Temporal Multi-Head Trend-Aware Attention.Its core innovation lies in IDGCN,which uniquely splits sequences into symmetric intervals for interactive feature sharing via dynamic graphs,and a novel attention mechanism incorporating convolutional operations to capture essential local traffic trends—addressing a critical gap in standard attention for continuous data.For 15-and 60-min forecasting on METR-LA,AIDGCN achieves MAEs of 0.75%and 0.39%,and RMSEs of 1.32%and 0.14%,respectively.In the 60-min long-term forecasting of the PEMS-BAY dataset,the AIDGCN out-performs the MRA-BGCN method by 6.28%,4.93%,and 7.17%in terms of MAE,RMSE,and MAPE,respectively.Experimental results demonstrate the superiority of our pro-posed model over state-of-the-art methods.展开更多
Multi-organ-on-a-chip(MOOC)technology represents a pivotal direction in the organ-on-a-chip field,seeking to emulate the complex interactions of multiple human organs in vitro through microfluidic systems.This technol...Multi-organ-on-a-chip(MOOC)technology represents a pivotal direction in the organ-on-a-chip field,seeking to emulate the complex interactions of multiple human organs in vitro through microfluidic systems.This technology overcomes the limitations of traditional single-organ models,providing a novel platform for investigating complex disease mechanisms and evaluating drug efficacy and toxicity.Although it demonstrates broad application prospects,its development still faces critical bottlenecks,including inadequate physiological coupling between organs,short functional maintenance durations,and limited real-time monitoring capabilities.Contemporary research is advancing along three key directions,including functional coupling,sensor integration,and full-process automation systems,to propel the technology toward enhanced levels of physiological relevance and predictive accuracy.展开更多
The running-in frictional experiments were conducted under simulated engine conditionswith actual piston rings and iron cylinder liner combinations. Elemental analyses of the wear tracksindicate that antiwear agent ZD...The running-in frictional experiments were conducted under simulated engine conditionswith actual piston rings and iron cylinder liner combinations. Elemental analyses of the wear tracksindicate that antiwear agent ZDTP is quite different from the Mg- and Ca-containing detergents inthe selectivity towards the tribosurfaces of both rings and liners. It is suggested that the tribo-chemical activity of tribosurfaces plays the role in governing the characteristic population of func-tional additive elements.展开更多
BACKGROUND Hepatic ischemia-reperfusion(I/R)injury related to liver transplantation and hepatic resection remains a challenge in clinical practice.Accumulating evidence indicates that mitochondrial dysfunction is a cr...BACKGROUND Hepatic ischemia-reperfusion(I/R)injury related to liver transplantation and hepatic resection remains a challenge in clinical practice.Accumulating evidence indicates that mitochondrial dysfunction is a critical cause of I/R injury.The protein 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1(NIPSNAP1)is involved in the regulation of mitophagy and the recruitment of autophagy receptor proteins independent of PTEN induced putative kinase 1.AIM To clarify the protective mechanism of NIPSNAP1 against hepatic I/R,with a focus on mitophagy and mitochondrial dynamics,as well as the potential mechanism by which n6-methyladenosine(m6A)modification regulates NIPSNAP1.METHODS Mice were administered an adeno-associated virus in vivo and a hepatic I/R model was established via portal vein interruption followed by reperfusion to explore the effect of NIPSNAP1 on hepatic I/R.HepG2 cells were subjected to hypoxia/reoxygenation treatment in vitro.RESULTS We observed a significant downregulation of both NIPSNAP1 and insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2)expression in vivo and in vitro.NIPSNAP1 knockdown impaired mitophagy and disrupted mitochondrial dynamics;in contrast,NIPSNAP1 overexpression resulted in the opposite effects.Further studies revealed that IGF2BP2 functions as an m6A reader that targets and binds NIPSNAP1,thereby regulating its mRNA stability.CONCLUSION NIPSNAP1 prevents hepatic I/R injury by promoting mitophagy and maintaining mitochondrial homeostasis,serving as a novel target of the m6A reader IGF2BP2.Therefore,targeting the IGF2BP2/NIPSNAP1 axis may facilitate the development of better therapeutics for hepatic I/R.展开更多
The primary mechanism of secondary injury after cerebral ischemia may be the brain inflammation that emerges after an ischemic stroke,which promotes neuronal death and inhibits nerve tissue regeneration.As the first i...The primary mechanism of secondary injury after cerebral ischemia may be the brain inflammation that emerges after an ischemic stroke,which promotes neuronal death and inhibits nerve tissue regeneration.As the first immune cells to be activated after an ischemic stroke,microglia play an important immunomodulatory role in the progression of the condition.After an ischemic stroke,peripheral blood immune cells(mainly T cells)are recruited to the central nervous system by chemokines secreted by immune cells in the brain,where they interact with central nervous system cells(mainly microglia)to trigger a secondary neuroimmune response.This review summarizes the interactions between T cells and microglia in the immune-inflammatory processes of ischemic stroke.We found that,during ischemic stroke,T cells and microglia demonstrate a more pronounced synergistic effect.Th1,Th17,and M1 microglia can co-secrete proinflammatory factors,such as interferon-γ,tumor necrosis factor-α,and interleukin-1β,to promote neuroinflammation and exacerbate brain injury.Th2,Treg,and M2 microglia jointly secrete anti-inflammatory factors,such as interleukin-4,interleukin-10,and transforming growth factor-β,to inhibit the progression of neuroinflammation,as well as growth factors such as brain-derived neurotrophic factor to promote nerve regeneration and repair brain injury.Immune interactions between microglia and T cells influence the direction of the subsequent neuroinflammation,which in turn determines the prognosis of ischemic stroke patients.Clinical trials have been conducted on the ways to modulate the interactions between T cells and microglia toward anti-inflammatory communication using the immunosuppressant fingolimod or overdosing with Treg cells to promote neural tissue repair and reduce the damage caused by ischemic stroke.However,such studies have been relatively infrequent,and clinical experience is still insufficient.In summary,in ischemic stroke,T cell subsets and activated microglia act synergistically to regulate inflammatory progression,mainly by secreting inflammatory factors.In the future,a key research direction for ischemic stroke treatment could be rooted in the enhancement of anti-inflammatory factor secretion by promoting the generation of Th2 and Treg cells,along with the activation of M2-type microglia.These approaches may alleviate neuroinflammation and facilitate the repair of neural tissues.展开更多
The E3 ubiquitin ligase,carboxyl terminus of heat shock protein 70(Hsp70)interacting protein(CHIP),also functions as a co-chaperone and plays a crucial role in the protein quality control system.In this study,we aimed...The E3 ubiquitin ligase,carboxyl terminus of heat shock protein 70(Hsp70)interacting protein(CHIP),also functions as a co-chaperone and plays a crucial role in the protein quality control system.In this study,we aimed to investigate the neuroprotective effect of overexpressed CHIP on Alzheimer’s disease.We used an adeno-associated virus vector that can cross the blood-brain barrier to mediate CHIP overexpression in APP/PS1 mouse brain.CHIP overexpression significantly ameliorated the performance of APP/PS1 mice in the Morris water maze and nest building tests,reduced amyloid-βplaques,and decreased the expression of both amyloid-βand phosphorylated tau.CHIP also alleviated the concentration of microglia and astrocytes around plaques.In APP/PS1 mice of a younger age,CHIP overexpression promoted an increase in ADAM10 expression and inhibitedβ-site APP cleaving enzyme 1,insulin degrading enzyme,and neprilysin expression.Levels of HSP70 and HSP40,which have functional relevance to CHIP,were also increased.Single nuclei transcriptome sequencing in the hippocampus of CHIP overexpressed mice showed that the lysosomal pathway and oligodendrocyte-related biological processes were up-regulated,which may also reflect a potential mechanism for the neuroprotective effect of CHIP.Our research shows that CHIP effectively reduces the behavior and pathological manifestations of APP/PS1 mice.Indeed,overexpression of CHIP could be a beneficial approach for the treatment of Alzheimer’s disease.展开更多
Rockbursts, which mainly affect mining roadways, are dynamic disasters arising from the surrounding rock under high stress. Understanding the interaction between supports and the surrounding rock is necessary for effe...Rockbursts, which mainly affect mining roadways, are dynamic disasters arising from the surrounding rock under high stress. Understanding the interaction between supports and the surrounding rock is necessary for effective rockburst control. In this study, the squeezing behavior of the surrounding rock is analyzed in rockburst roadways, and a mechanical model of rockbursts is established considering the dynamic support stress, thus deriving formulas and providing characteristic curves for describing the interaction between the support and surrounding rock. Design principles and parameters of supports for rockburst control are proposed. The results show that only when the geostress magnitude exceeds a critical value can it drive the formation of rockburst conditions. The main factors influencing the convergence response and rockburst occurrence around roadways are geostress, rock brittleness, uniaxial compressive strength, and roadway excavation size. Roadway support devices can play a role in controlling rockburst by suppressing the squeezing evolution of the surrounding rock towards instability points of rockburst. Further, the higher the strength and the longer the impact stroke of support devices with constant resistance, the more easily multiple balance points can be formed with the surrounding rock to control rockburst occurrence. Supports with long impact stroke allow adaptation to varying geostress levels around the roadway, aiding in rockburst control. The results offer a quantitative method for designing support systems for rockburst-prone roadways. The design criterion of supports is determined by the intersection between the convergence curve of the surrounding rock and the squeezing deformation curve of the support devices.展开更多
The Late Cretaceous Jiepailing granitoids,located at the central Nanling Range in South China,are closely associated with significant Sn-Li-Be-F polymetallic metallogeny.The Jiepailing granitoids mainly consist of gra...The Late Cretaceous Jiepailing granitoids,located at the central Nanling Range in South China,are closely associated with significant Sn-Li-Be-F polymetallic metallogeny.The Jiepailing granitoids mainly consist of granitic porphyry and zinnwaldite granite.The two granitoids have an A-type affinity,showing elevated Rb/Sr ratios and significant depletions in Ba,Sr and P.Integrated zircon and monazite U-Pb dating results suggest that granitic porphyry and zinnwaldite granite were emplaced at~89 Ma and~94 Ma,respectively.The low Ce^(4+)/Ce^(3+)ratios of the Jiepailing granitoids,together with significant negative Eu anomalies of the zircons,indicate that their formation occurred under conditions of reduced oxygen fugacity.Through the analysis of zircon Hf-O and whole-rock Nd isotopes,it has been determined that both stages of the Jiepailing granitoids originated in the lower-middle Mesoproterozoic crustal basement[ε_(Nd)(t)=−5.33 to−4.96,t^(C)_(DM)(Nd)=1289-1234 Ma,ε_(Hf)(t)=−4.13 to+2.22,t^(C)_(DM)(Hf)=1418-1015 Ma andδ^(18)O_(Zrc)=6.33‰-7.72‰],with the involvement of mantle-derived materials.Both granitic porphyry and zinnwaldite granite exhibit elevated concentrations of fluorine(F),with the positive correlation between F and Sn emphasizing the crucial role of high F sources in tin mineralization.Drawing upon the study of the Late Cretaceous magma systems in southern Hunan and through comparison with the mineralized granites observed in coastal regions during the Late Cretaceous,a genetic model for the mineralized granites in the Nanling region is developed.When the Paleo-Pacific Plate retreated to the coastal region,the continental crust in southern China underwent extensional thinning and asthenospheric upwelling due to gravitational collapse.Such processes resulted in the partial melting of the middle-lower crustal metamorphic sedimentary basement and the subsequent formation of F-rich granitic magmas,related to tin mineralization.展开更多
To examine the similarities and differences in the evolution of cavity,wetting and dynamics of a highspeed,oblique water-entry projectile with different positive angles of attack,a comparative analysis has been conduc...To examine the similarities and differences in the evolution of cavity,wetting and dynamics of a highspeed,oblique water-entry projectile with different positive angles of attack,a comparative analysis has been conducted based on the numerical results of two mathematical models,the rigid-body model and fluid-structure interaction model.In addition,the applicable scope of the above two methods,and the structural response characteristics of the projectile have also been investigated.Our results demonstrate that:(1) The impact loads and angular motion of the projectile of the rigid-body method are more likely to exhibit periodic variations due to the periodic tail slap,its range of positive angles of attack is about α<2°.(2) When the projectile undergone significant wetting,a strong coupling effect is observed among wetting,structural deformation,and projectile motion.With the applied projectile shape,it is observed that,when the projectile bends,the final wetting position is that of Part B(cylinder of body).With the occu rrence of this phenomenon,the projectile ballistics beco me completely unstable.(3) The force exerted on the lower surface of the projectile induced by wetting is the primary reason of the destabilization of the projectile traj ectory and structu ral deformation failure.Bending deformation is most likely to appear at the junction of Part C(cone of body) and Part D(tail).The safe angles of attack of the projectile stability are found to be about α≤2°.展开更多
Objective Oral squamous cell carcinoma(OSCC)is an aggressive cancer with a high mortality rate.San-Zhong-Kui-Jian-Tang(SZKJT),a Chinese herbal formula,has long been used as an adjuvant therapy in cancer clinical pract...Objective Oral squamous cell carcinoma(OSCC)is an aggressive cancer with a high mortality rate.San-Zhong-Kui-Jian-Tang(SZKJT),a Chinese herbal formula,has long been used as an adjuvant therapy in cancer clinical practice.Although its therapeutic effects and molecular mechanisms in OSCC have been previously elucidated,the potential interactions and mechanisms between the active phytochemicals and their therapeutic targets are still lacking.Methods The present study employed network pharmacology and topology approaches to establish a“herbal ingredients–active phytochemicals–target interaction”network to explore the potential therapeutic targets of SZKJT-active phytochemicals in the treatment of OSCC.The role of the target proteins in oncogenesis was assessed via GO and KEGG enrichment analyses,and their interactions with the active phytochemicals of SZKJT were calculated via molecular docking and dynamic simulations.The pharmacokinetic properties and toxicity of the active phytochemicals were also predicted.Results A total of 171 active phytochemicals of SZKJT fulfilled the bioavailability and drug-likeness screening criteria,with the flavonoids quercetin,kaempferol,and naringenin having the greatest potential.The 4 crucial targets of these active phytochemicals are PTGS2,TNF,BCL2,and CASP3,which encode cyclooxygenase-2,tumor necrosis factor(TNF),BCL-2 apoptosis regulator,and caspase-3,respectively.The interactions between phytochemicals and target proteins were predicted to be thermodynamically feasible and stable via molecular docking and dynamics simulations.Finally,the results revealed that the IL-6/JAK/STAT3 pathway and TNF signaling via NF-κB are the two prominent pathways targeted by SZKJT.Conclusion In summary,this study provides computational data for in-depth exploration of the mechanism by which SZKJT activates phytochemicals to treat OSCC.展开更多
Deficiency or restriction of Zn absorption in soils is one of the most common micronutrients deficient in cereal plants. To investigate critical micronutrient interaction in zinc deficiency and zinc sufficient in soil...Deficiency or restriction of Zn absorption in soils is one of the most common micronutrients deficient in cereal plants. To investigate critical micronutrient interaction in zinc deficiency and zinc sufficient in soil, a factorial experiment based on completely randomized design (CRD) with three replications was conducted in 2023. Six wheat cultivars with different Zn efficiency were used. The cultivars were grown under Zn deficiency and adequate conditions. Results showed that in Zn deficiency conditions, with increasing Zn concentration in the roots, Fe concentrations were increased too, while the Cu and Mn concentrations decreased. In the same condition and with increasing Zn concentration in shoots, the concentrations of Fe and Mn decreased, while Cu were increased. However, by increasing Zn concentration, Fe, Cu, and Mn concentrations were increased in Zn deficiency condition in grains, as well as Zn sufficient conditions. RST (root to shoot micronutrient translocation) comparison of cultivars showed that in lack of Zn, the ability of translocation of Zn, Fe, and Mn in Zn-inefficient cultivar from root to shoot was higher than inefficient cultivar. In the same conditions, the capability of Zn-inefficient cultivar in Cu translocation from root to shoot was lower than other cultivars. In general, it seems that in Zn deficiency conditions, there are antagonistic effects among Zn, Cu and Mn and synergistic effects between Zn and Fe in the root. Also, in Zn sufficient conditions, there were synergistic effects among all studies micronutrients which include Zn, Fe, Cu, and Mn.展开更多
The global reliance on phosphate rock for agriculture and other industries,coupled with chemical regulations in developed countries,has driven the search for green alternatives in apatite flotation.This review investi...The global reliance on phosphate rock for agriculture and other industries,coupled with chemical regulations in developed countries,has driven the search for green alternatives in apatite flotation.This review investigates eco-friendly collectors’effectiveness in promoting sustainable mineral processing,guiding future alternatives to traditional reagents.The manuscript discussed the surface properties of apatite and its interaction with eco-friendly collectors,assessing existing fundamental studies.This study sought to:(1)define,organize,and classify“eco-friendly”collectors;(2)evaluate their effect in IEP and contact angle;(3)provide a better understanding of the adsorption behavior of the different fatty acid chains into apatite surface;(4)assess their ability to reversely and directly float apatite;(5)address gaps to achieve selectivity and process optimization.Outcomes demonstrated that fatty acids are largely applied,but other renewable sources of these reagents have been promisingly evaluated.In addition,other natural reagents have been tested,and new green synthetics have demonstrated synergistic effects when combined with fatty acids,yielding significant improvements in grade and recovery.However,collector effectiveness varies with ore characteristics,like particle size and surface properties,which remain underexplored.Future research should design tailored collectors that align with mineralogical differences to enhance selectivity.展开更多
Epilepsy affects over 50 million people worldwide.Drug-resistant epilepsy(DRE)accounts for up to a third of these cases,and neuro-inflammation is thought to play a role in such cases.Despite being a long-debated issue...Epilepsy affects over 50 million people worldwide.Drug-resistant epilepsy(DRE)accounts for up to a third of these cases,and neuro-inflammation is thought to play a role in such cases.Despite being a long-debated issue in the field of DRE,the mechanisms underlying neuroinflammation have yet to be fully elucidated.The pro-inflammatory microenvironment within the brain tissue of people with DRE has been probed using single-cell multimodal transcriptomics.Evidence suggests that inflammatory cells and pro-inflammatory cytokines in the nervous system can lead to extensive biochemical changes,such as connexin hemichannel excitability and disruption of neurotransmitter homeostasis.The presence of inflammation may give rise to neuronal network abnormalities that suppress endogenous antiepileptic systems.We focus on the role of neuroinflammation and brain network anomalies in DRE from multiple perspectives to identify critical points for clinical application.We hope to provide an insightful overview to advance the quest for better DRE treatments.展开更多
基金supported by the National Basic Research Program of China("973"Project)(Grant No.2013CB036203)the National Natural Science Foundation of China(Grant No.U1134206)+1 种基金the 111 project(Grant No.B13002)the Doctoral Fund of Ministry of Education of China(Grant No.20130009110036)
文摘Considering the CRTS-II track slab,which is commonly used in the Chinese high-speed railway system,a vehicle-track-bridge dynamic analysis method is proposed in which the vehicle subsystem equations are established by the rigid body dynamics method,the track subsystem and the bridge subsystem equations are established by the FEM,the wheel-rail contact relation is defined by the corresponding assumption in vertical direction and the Kalker linear creep theory in lateral direction.The in-span spring element is derived to model the track-bridge interaction;the equal-band-width storage is adopted to fit the track structure with multilayer uniform section beam;and the dynamic equilibrium equations are solved by the inter-history iteration method.As a case study,the response of a CRH2 high-speed train transverses a simply-supported bridge with successive 31.5m double bound pre-stress beams is simulated.The result shows that using the vehicle-track-bridge interaction model instead of the vehicle-bridge interaction model helps predict the rotation angle at beam ends and choose an economic beam vertical stiffness.
文摘In order to resolve the multisensor multiplied maneuvering target tracking problem, this paper presents a distributed interacted multiple model multisensor joint probabilistic data association algorithm （DIMM-MSJPDA）. First of all, the interacted multiple model joint probabilistic data association algorithm is applied to each sensor, and then the state estimation, estimation covariance, model probability, combined innovation, innovation covariance are delivered to the fusion center. Then, the tracks from each sensor are correlated and the D-S evidence theory is used to gain the model probability of an identical target. Finally, the ultimate state estimation of each target is calculated according to the new model probability, and the state estimation is transmitted to each sensor. Simulations are designed to test the tracking performance of DIMM-MSJPDA algorithm. The results show that the use of DIMM-MSJPDA algorithm enables the distributed multisensor system to track multiplied maneuvering targets and its tracking performance is much better than that of IMMJPDA algorithm.
基金supported by JSPS(KAKENHI:21K06205,23K06937,24K23419)AMED(to JYK,SaY,TM,SiY,YT,and NH)JYW had long been supported by the NIH.
文摘The nervous system processes a vast amount of information,performing computations that underlie perception,cognition,and behavior.During development,neuronal guidance genes,which encode extracellular cues,their receptors,and downstream signal transducers,organize neural wiring to generate the complex architecture of the nervous system.It is now evident that many of these neuroguidance cues and their receptors are active during development and are also expressed in the adult nervous system.This suggests that neuronal guidance pathways are critical not only for neural wiring but also for ongoing function and maintenance of the mature nervous system.Supporting this view,these pathways continue to regulate synaptic connectivity,plasticity,and remodeling,and overall brain homeostasis throughout adulthood.Genetic and transcriptomic analyses have further revealed many neuronal guidance genes to be associated with a wide range of neurodegenerative and neuropsychiatric disorders.Although the precise mechanisms by which aberrant neuronal guidance signaling drives the pathogenesis of these diseases remain to be clarified,emerging evidence points to several common themes,including dysfunction in neurons,microglia,astrocytes,and endothelial cells,along with dysregulation of neuron-microglia-astrocyte,neuroimmune,and neurovascular interactions.In this review,we explore recent advances in understanding the molecular and cellular mechanisms by which aberrant neuronal guidance signaling contributes to disease pathogenesis through altered cell-cell interactions.For instance,recent studies have unveiled two distinct semaphorin-plexin signaling pathways that affect microglial activation and neuroinflammation.We discuss the challenges ahead,along with the therapeutic potentials of targeting neuronal guidance pathways for treating neurodegenerative diseases.Particular focus is placed on how neuronal guidance mechanisms control neuron-glia and neuroimmune interactions and modulate microglial function under physiological and pathological conditions.Specifically,we examine the crosstalk between neuronal guidance signaling and TREM2,a master regulator of microglial function,in the context of pathogenic protein aggregates.It is well-established that age is a major risk factor for neurodegeneration.Future research should address how aging and neuronal guidance signaling interact to influence an individual’s susceptibility to various late-onset neurological diseases and how the progression of these diseases could be therapeutically blocked by targeting neuronal guidance pathways.
基金funding by National Natural Science Foundation of China(No.82174492)National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion Project(N o.ZJJBGS2024002-1).
文摘Background: The human gut microbiome is an important target for disease treatment and prevention. Various microbial species within the complex ecosystem of the microbiome have been shown to play important roles in disease. Identification of bioactive materials capable of altering the abundances of these species both safely and effectively is a major goal in microbiome research. Many traditional Chinese medicines (TCMs) have been reported to affect the composition of the gut microbiome. Here, we summarize studies that have used TCMs to alter the gut microbiome and discuss the response relationship between TCMs and gut microbial species. Methods: We searched the PubMed, Web of Science, and Knowledge Network databases using the terms “traditional Chinese medicine,” “gut microbiome,” and specific system disease names (endocrine, immune, nervous, cardiovascular, and digestive). Studies were excluded if irrelevant or if the experimental procedures were unclear. Results: TCMs have been reported to affect a wide range of gut microbial taxa spanning major phyla, including Firmicutes, Bacteroidetes, Proteobacteria, Verrucomicrobiota, Actinobacteria, and Fusobacteria. In all, 54 TCMs including compounds and extracts have been tested in rodents and 30 have been examined in human trials. Almost all studies have reported positive results in regulating the gut microbiome as well as modulating corresponding phenotypes, spanning diseases of the endocrine, immune, nervous, cardiovascular, and digestive systems. Gut species, including Akkermansia, Bacteroides, Fusobacterium, Faecalibacterium, and E. coli, were found to be regulated by 19 TCMs. A network was constructed to visualize the interactions between TCMs and these taxa. Conclusion: There exists a complex and close relationship between intestinal microflora and diseases. Sufficient experimental data and studies have proved that the imbalance of intestinal microflora affects health by mediating metabolism, immune regulation, inflammation and signal transduction. Many characteristic alterations of intestinal microflora are positively correlated with diseases, so intestinal microflora has become a potential risk index and treatment target for many diseases. Many TCMs affect the relative abundances of microbial species in the gut, and therefore may be useful for modulating the gut microbiome. This review provides a reference for prioritizing candidate TCMs from the enormous repertoire of such medicines to test which specific gut microbes are targeted.
基金Supported by the Development and Application Project of Ship CAE Software.
文摘Studies of wave-current interactions are vital for the safe design of structures.Regular waves in the presence of uniform,linear shear,and quadratic shear currents are explored by the High-Level Green-Naghdi model in this paper.The five-point central difference method is used for spatial discretization,and the fourth-order Adams predictor-corrector scheme is employed for marching in time.The domain-decomposition method is applied for the wave-current generation and absorption.The effects of currents on the wave profile and velocity field are examined under two conditions:the same velocity of currents at the still-water level and the constant flow volume of currents.Wave profiles and velocity fields demonstrate substantial differences in three types of currents owing to the diverse vertical distribution of current velocity and vorticity.Then,loads on small-scale vertical cylinders subjected to regular waves and three types of background currents with the same flow volume are investigated.The maximum load intensity and load fluctuation amplitude in uniform,linear shear,and quadratic shear currents increase sequentially.The stretched superposition method overestimates the maximum load intensity and load fluctuation amplitude in opposing currents and underestimates these values in following currents.The stretched superposition method obtains a poor approximation for strong nonlinear waves,particularly in the case of the opposing quadratic shear current.
基金supported by the National Key R&D Program of China(Grant No.2023YFA1406200)the National Natural Science Foundation of China(No.12274177 and 12304261)the China Postdoctoral Science Foundation(No.2024M751076)。
文摘Luminescent metal-organic frameworks(MOFs)have garnered significant attention due to their structural tunability and potential applications in solid-state lighting,bioimaging,sensing,anticounterfeiting,and other fields.Nevertheless,due to the tendency of1,4-benzenedicarboxylic acid(BDC)to rotate within the framework,MOFs composed of it exhibit significant non-radiative energy dissipation and thus impair the emissive properties.In this study,efficient luminescence of MIL-140A nanocrystals(NCs)with BDC rotors as ligands is achieved by pressure treatment strategy.Pressure treatment effectively modulates the pore structure of the framework,enhancing the interactions between the N,N-dimethylformamide vip molecules and the BDC ligands.The enhanced host-vip interaction contributes to the structural rigidity of the MOF,thereby suppressing the rotation-induced excited-state energy loss.As a result,the pressure-treated MIL-140A NCs displayed bright blue-light emission,with the photoluminescence quantum yield increasing from an initial 6.8%to 69.2%.This study developed an effective strategy to improve the luminescence performance of rotor ligand MOFs,offers a new avenue for the rational design and synthesis of MOFs with superior luminescent properties.
文摘Reliable traffic flow prediction is crucial for mitigating urban congestion.This paper proposes Attentionbased spatiotemporal Interactive Dynamic Graph Convolutional Network(AIDGCN),a novel architecture integrating Interactive Dynamic Graph Convolution Network(IDGCN)with Temporal Multi-Head Trend-Aware Attention.Its core innovation lies in IDGCN,which uniquely splits sequences into symmetric intervals for interactive feature sharing via dynamic graphs,and a novel attention mechanism incorporating convolutional operations to capture essential local traffic trends—addressing a critical gap in standard attention for continuous data.For 15-and 60-min forecasting on METR-LA,AIDGCN achieves MAEs of 0.75%and 0.39%,and RMSEs of 1.32%and 0.14%,respectively.In the 60-min long-term forecasting of the PEMS-BAY dataset,the AIDGCN out-performs the MRA-BGCN method by 6.28%,4.93%,and 7.17%in terms of MAE,RMSE,and MAPE,respectively.Experimental results demonstrate the superiority of our pro-posed model over state-of-the-art methods.
基金supported by the Shenzhen Medical Research Fund(Grant No.A2303049)Guangdong Basic and Applied Basic Research(Grant No.2023A1515010647)+1 种基金National Natural Science Foundation of China(Grant No.22004135)Shenzhen Science and Technology Program(Grant No.RCBS20210706092409020,GXWD20201231165807008,20200824162253002).
文摘Multi-organ-on-a-chip(MOOC)technology represents a pivotal direction in the organ-on-a-chip field,seeking to emulate the complex interactions of multiple human organs in vitro through microfluidic systems.This technology overcomes the limitations of traditional single-organ models,providing a novel platform for investigating complex disease mechanisms and evaluating drug efficacy and toxicity.Although it demonstrates broad application prospects,its development still faces critical bottlenecks,including inadequate physiological coupling between organs,short functional maintenance durations,and limited real-time monitoring capabilities.Contemporary research is advancing along three key directions,including functional coupling,sensor integration,and full-process automation systems,to propel the technology toward enhanced levels of physiological relevance and predictive accuracy.
文摘The running-in frictional experiments were conducted under simulated engine conditionswith actual piston rings and iron cylinder liner combinations. Elemental analyses of the wear tracksindicate that antiwear agent ZDTP is quite different from the Mg- and Ca-containing detergents inthe selectivity towards the tribosurfaces of both rings and liners. It is suggested that the tribo-chemical activity of tribosurfaces plays the role in governing the characteristic population of func-tional additive elements.
基金Supported by the National Natural Science Foundation of China,No.82200658.
文摘BACKGROUND Hepatic ischemia-reperfusion(I/R)injury related to liver transplantation and hepatic resection remains a challenge in clinical practice.Accumulating evidence indicates that mitochondrial dysfunction is a critical cause of I/R injury.The protein 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1(NIPSNAP1)is involved in the regulation of mitophagy and the recruitment of autophagy receptor proteins independent of PTEN induced putative kinase 1.AIM To clarify the protective mechanism of NIPSNAP1 against hepatic I/R,with a focus on mitophagy and mitochondrial dynamics,as well as the potential mechanism by which n6-methyladenosine(m6A)modification regulates NIPSNAP1.METHODS Mice were administered an adeno-associated virus in vivo and a hepatic I/R model was established via portal vein interruption followed by reperfusion to explore the effect of NIPSNAP1 on hepatic I/R.HepG2 cells were subjected to hypoxia/reoxygenation treatment in vitro.RESULTS We observed a significant downregulation of both NIPSNAP1 and insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2)expression in vivo and in vitro.NIPSNAP1 knockdown impaired mitophagy and disrupted mitochondrial dynamics;in contrast,NIPSNAP1 overexpression resulted in the opposite effects.Further studies revealed that IGF2BP2 functions as an m6A reader that targets and binds NIPSNAP1,thereby regulating its mRNA stability.CONCLUSION NIPSNAP1 prevents hepatic I/R injury by promoting mitophagy and maintaining mitochondrial homeostasis,serving as a novel target of the m6A reader IGF2BP2.Therefore,targeting the IGF2BP2/NIPSNAP1 axis may facilitate the development of better therapeutics for hepatic I/R.
基金supported by the National Natural Science Foundation of China,Nos.82104560(to CL),U21A20400(to QW)the Natural Science Foundation of Beijing,No.7232279(to XW)the Project of Beijing University of Chinese Medicine,No.2022-JYB-JBZR-004(to XW)。
文摘The primary mechanism of secondary injury after cerebral ischemia may be the brain inflammation that emerges after an ischemic stroke,which promotes neuronal death and inhibits nerve tissue regeneration.As the first immune cells to be activated after an ischemic stroke,microglia play an important immunomodulatory role in the progression of the condition.After an ischemic stroke,peripheral blood immune cells(mainly T cells)are recruited to the central nervous system by chemokines secreted by immune cells in the brain,where they interact with central nervous system cells(mainly microglia)to trigger a secondary neuroimmune response.This review summarizes the interactions between T cells and microglia in the immune-inflammatory processes of ischemic stroke.We found that,during ischemic stroke,T cells and microglia demonstrate a more pronounced synergistic effect.Th1,Th17,and M1 microglia can co-secrete proinflammatory factors,such as interferon-γ,tumor necrosis factor-α,and interleukin-1β,to promote neuroinflammation and exacerbate brain injury.Th2,Treg,and M2 microglia jointly secrete anti-inflammatory factors,such as interleukin-4,interleukin-10,and transforming growth factor-β,to inhibit the progression of neuroinflammation,as well as growth factors such as brain-derived neurotrophic factor to promote nerve regeneration and repair brain injury.Immune interactions between microglia and T cells influence the direction of the subsequent neuroinflammation,which in turn determines the prognosis of ischemic stroke patients.Clinical trials have been conducted on the ways to modulate the interactions between T cells and microglia toward anti-inflammatory communication using the immunosuppressant fingolimod or overdosing with Treg cells to promote neural tissue repair and reduce the damage caused by ischemic stroke.However,such studies have been relatively infrequent,and clinical experience is still insufficient.In summary,in ischemic stroke,T cell subsets and activated microglia act synergistically to regulate inflammatory progression,mainly by secreting inflammatory factors.In the future,a key research direction for ischemic stroke treatment could be rooted in the enhancement of anti-inflammatory factor secretion by promoting the generation of Th2 and Treg cells,along with the activation of M2-type microglia.These approaches may alleviate neuroinflammation and facilitate the repair of neural tissues.
基金supported by the National Natural Science Foundation of China,Nos.91849115 and U1904207(to YX),81974211 and 82171247(to CS)Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences,No.2020-PT310-01(to YX).
文摘The E3 ubiquitin ligase,carboxyl terminus of heat shock protein 70(Hsp70)interacting protein(CHIP),also functions as a co-chaperone and plays a crucial role in the protein quality control system.In this study,we aimed to investigate the neuroprotective effect of overexpressed CHIP on Alzheimer’s disease.We used an adeno-associated virus vector that can cross the blood-brain barrier to mediate CHIP overexpression in APP/PS1 mouse brain.CHIP overexpression significantly ameliorated the performance of APP/PS1 mice in the Morris water maze and nest building tests,reduced amyloid-βplaques,and decreased the expression of both amyloid-βand phosphorylated tau.CHIP also alleviated the concentration of microglia and astrocytes around plaques.In APP/PS1 mice of a younger age,CHIP overexpression promoted an increase in ADAM10 expression and inhibitedβ-site APP cleaving enzyme 1,insulin degrading enzyme,and neprilysin expression.Levels of HSP70 and HSP40,which have functional relevance to CHIP,were also increased.Single nuclei transcriptome sequencing in the hippocampus of CHIP overexpressed mice showed that the lysosomal pathway and oligodendrocyte-related biological processes were up-regulated,which may also reflect a potential mechanism for the neuroprotective effect of CHIP.Our research shows that CHIP effectively reduces the behavior and pathological manifestations of APP/PS1 mice.Indeed,overexpression of CHIP could be a beneficial approach for the treatment of Alzheimer’s disease.
基金funded by the National Natural Science Foundation of China (No. 52304133)the National Key R&D Program of China (No. 2022YFC3004605)the Department of Science and Technology of Liaoning Province (No. 2023-BS-083)。
文摘Rockbursts, which mainly affect mining roadways, are dynamic disasters arising from the surrounding rock under high stress. Understanding the interaction between supports and the surrounding rock is necessary for effective rockburst control. In this study, the squeezing behavior of the surrounding rock is analyzed in rockburst roadways, and a mechanical model of rockbursts is established considering the dynamic support stress, thus deriving formulas and providing characteristic curves for describing the interaction between the support and surrounding rock. Design principles and parameters of supports for rockburst control are proposed. The results show that only when the geostress magnitude exceeds a critical value can it drive the formation of rockburst conditions. The main factors influencing the convergence response and rockburst occurrence around roadways are geostress, rock brittleness, uniaxial compressive strength, and roadway excavation size. Roadway support devices can play a role in controlling rockburst by suppressing the squeezing evolution of the surrounding rock towards instability points of rockburst. Further, the higher the strength and the longer the impact stroke of support devices with constant resistance, the more easily multiple balance points can be formed with the surrounding rock to control rockburst occurrence. Supports with long impact stroke allow adaptation to varying geostress levels around the roadway, aiding in rockburst control. The results offer a quantitative method for designing support systems for rockburst-prone roadways. The design criterion of supports is determined by the intersection between the convergence curve of the surrounding rock and the squeezing deformation curve of the support devices.
基金financially supported by the National Natural Science Foundation of China(Grant No.42303073).
文摘The Late Cretaceous Jiepailing granitoids,located at the central Nanling Range in South China,are closely associated with significant Sn-Li-Be-F polymetallic metallogeny.The Jiepailing granitoids mainly consist of granitic porphyry and zinnwaldite granite.The two granitoids have an A-type affinity,showing elevated Rb/Sr ratios and significant depletions in Ba,Sr and P.Integrated zircon and monazite U-Pb dating results suggest that granitic porphyry and zinnwaldite granite were emplaced at~89 Ma and~94 Ma,respectively.The low Ce^(4+)/Ce^(3+)ratios of the Jiepailing granitoids,together with significant negative Eu anomalies of the zircons,indicate that their formation occurred under conditions of reduced oxygen fugacity.Through the analysis of zircon Hf-O and whole-rock Nd isotopes,it has been determined that both stages of the Jiepailing granitoids originated in the lower-middle Mesoproterozoic crustal basement[ε_(Nd)(t)=−5.33 to−4.96,t^(C)_(DM)(Nd)=1289-1234 Ma,ε_(Hf)(t)=−4.13 to+2.22,t^(C)_(DM)(Hf)=1418-1015 Ma andδ^(18)O_(Zrc)=6.33‰-7.72‰],with the involvement of mantle-derived materials.Both granitic porphyry and zinnwaldite granite exhibit elevated concentrations of fluorine(F),with the positive correlation between F and Sn emphasizing the crucial role of high F sources in tin mineralization.Drawing upon the study of the Late Cretaceous magma systems in southern Hunan and through comparison with the mineralized granites observed in coastal regions during the Late Cretaceous,a genetic model for the mineralized granites in the Nanling region is developed.When the Paleo-Pacific Plate retreated to the coastal region,the continental crust in southern China underwent extensional thinning and asthenospheric upwelling due to gravitational collapse.Such processes resulted in the partial melting of the middle-lower crustal metamorphic sedimentary basement and the subsequent formation of F-rich granitic magmas,related to tin mineralization.
基金supported by the Postgraduate Research&Practice Innovation Program of Jiangsu Province(Grant No.KYCX24_0714).
文摘To examine the similarities and differences in the evolution of cavity,wetting and dynamics of a highspeed,oblique water-entry projectile with different positive angles of attack,a comparative analysis has been conducted based on the numerical results of two mathematical models,the rigid-body model and fluid-structure interaction model.In addition,the applicable scope of the above two methods,and the structural response characteristics of the projectile have also been investigated.Our results demonstrate that:(1) The impact loads and angular motion of the projectile of the rigid-body method are more likely to exhibit periodic variations due to the periodic tail slap,its range of positive angles of attack is about α<2°.(2) When the projectile undergone significant wetting,a strong coupling effect is observed among wetting,structural deformation,and projectile motion.With the applied projectile shape,it is observed that,when the projectile bends,the final wetting position is that of Part B(cylinder of body).With the occu rrence of this phenomenon,the projectile ballistics beco me completely unstable.(3) The force exerted on the lower surface of the projectile induced by wetting is the primary reason of the destabilization of the projectile traj ectory and structu ral deformation failure.Bending deformation is most likely to appear at the junction of Part C(cone of body) and Part D(tail).The safe angles of attack of the projectile stability are found to be about α≤2°.
文摘Objective Oral squamous cell carcinoma(OSCC)is an aggressive cancer with a high mortality rate.San-Zhong-Kui-Jian-Tang(SZKJT),a Chinese herbal formula,has long been used as an adjuvant therapy in cancer clinical practice.Although its therapeutic effects and molecular mechanisms in OSCC have been previously elucidated,the potential interactions and mechanisms between the active phytochemicals and their therapeutic targets are still lacking.Methods The present study employed network pharmacology and topology approaches to establish a“herbal ingredients–active phytochemicals–target interaction”network to explore the potential therapeutic targets of SZKJT-active phytochemicals in the treatment of OSCC.The role of the target proteins in oncogenesis was assessed via GO and KEGG enrichment analyses,and their interactions with the active phytochemicals of SZKJT were calculated via molecular docking and dynamic simulations.The pharmacokinetic properties and toxicity of the active phytochemicals were also predicted.Results A total of 171 active phytochemicals of SZKJT fulfilled the bioavailability and drug-likeness screening criteria,with the flavonoids quercetin,kaempferol,and naringenin having the greatest potential.The 4 crucial targets of these active phytochemicals are PTGS2,TNF,BCL2,and CASP3,which encode cyclooxygenase-2,tumor necrosis factor(TNF),BCL-2 apoptosis regulator,and caspase-3,respectively.The interactions between phytochemicals and target proteins were predicted to be thermodynamically feasible and stable via molecular docking and dynamics simulations.Finally,the results revealed that the IL-6/JAK/STAT3 pathway and TNF signaling via NF-κB are the two prominent pathways targeted by SZKJT.Conclusion In summary,this study provides computational data for in-depth exploration of the mechanism by which SZKJT activates phytochemicals to treat OSCC.
文摘Deficiency or restriction of Zn absorption in soils is one of the most common micronutrients deficient in cereal plants. To investigate critical micronutrient interaction in zinc deficiency and zinc sufficient in soil, a factorial experiment based on completely randomized design (CRD) with three replications was conducted in 2023. Six wheat cultivars with different Zn efficiency were used. The cultivars were grown under Zn deficiency and adequate conditions. Results showed that in Zn deficiency conditions, with increasing Zn concentration in the roots, Fe concentrations were increased too, while the Cu and Mn concentrations decreased. In the same condition and with increasing Zn concentration in shoots, the concentrations of Fe and Mn decreased, while Cu were increased. However, by increasing Zn concentration, Fe, Cu, and Mn concentrations were increased in Zn deficiency condition in grains, as well as Zn sufficient conditions. RST (root to shoot micronutrient translocation) comparison of cultivars showed that in lack of Zn, the ability of translocation of Zn, Fe, and Mn in Zn-inefficient cultivar from root to shoot was higher than inefficient cultivar. In the same conditions, the capability of Zn-inefficient cultivar in Cu translocation from root to shoot was lower than other cultivars. In general, it seems that in Zn deficiency conditions, there are antagonistic effects among Zn, Cu and Mn and synergistic effects between Zn and Fe in the root. Also, in Zn sufficient conditions, there were synergistic effects among all studies micronutrients which include Zn, Fe, Cu, and Mn.
基金financially supported by CAMM(Center of Advanced Mining and Metallurgy/Green Flotation),as a center of excellence at the Luleå University of Technology.
文摘The global reliance on phosphate rock for agriculture and other industries,coupled with chemical regulations in developed countries,has driven the search for green alternatives in apatite flotation.This review investigates eco-friendly collectors’effectiveness in promoting sustainable mineral processing,guiding future alternatives to traditional reagents.The manuscript discussed the surface properties of apatite and its interaction with eco-friendly collectors,assessing existing fundamental studies.This study sought to:(1)define,organize,and classify“eco-friendly”collectors;(2)evaluate their effect in IEP and contact angle;(3)provide a better understanding of the adsorption behavior of the different fatty acid chains into apatite surface;(4)assess their ability to reversely and directly float apatite;(5)address gaps to achieve selectivity and process optimization.Outcomes demonstrated that fatty acids are largely applied,but other renewable sources of these reagents have been promisingly evaluated.In addition,other natural reagents have been tested,and new green synthetics have demonstrated synergistic effects when combined with fatty acids,yielding significant improvements in grade and recovery.However,collector effectiveness varies with ore characteristics,like particle size and surface properties,which remain underexplored.Future research should design tailored collectors that align with mineralogical differences to enhance selectivity.
基金supported by the National Natural Science Foundation of China(82030037)the Translational and Application Project of Brain-inspired and Network Neuroscience on Brain Disorders(11000023T000002036286).
文摘Epilepsy affects over 50 million people worldwide.Drug-resistant epilepsy(DRE)accounts for up to a third of these cases,and neuro-inflammation is thought to play a role in such cases.Despite being a long-debated issue in the field of DRE,the mechanisms underlying neuroinflammation have yet to be fully elucidated.The pro-inflammatory microenvironment within the brain tissue of people with DRE has been probed using single-cell multimodal transcriptomics.Evidence suggests that inflammatory cells and pro-inflammatory cytokines in the nervous system can lead to extensive biochemical changes,such as connexin hemichannel excitability and disruption of neurotransmitter homeostasis.The presence of inflammation may give rise to neuronal network abnormalities that suppress endogenous antiepileptic systems.We focus on the role of neuroinflammation and brain network anomalies in DRE from multiple perspectives to identify critical points for clinical application.We hope to provide an insightful overview to advance the quest for better DRE treatments.
