Aim:The effects of tirofiban combined with nicorandil on effective reperfusion,and the levels of interleukin-4(IL-4)and soluble intercellular adhesion molecule-1(sICAM-1)after percutaneous coronary intervention(PCI)fo...Aim:The effects of tirofiban combined with nicorandil on effective reperfusion,and the levels of interleukin-4(IL-4)and soluble intercellular adhesion molecule-1(sICAM-1)after percutaneous coronary intervention(PCI)for chronic coronary total occlusion(CTO)were investigated.Method:From January 1,2017,to June 31,2019,a total of 40 patients with CTO receiving PCI in Shandong Qian-foshan Hospital were randomly divided into a control group(treated with single tirofiban)and a cocktail group(treated with nicorandil combined with tirofiban).Effective reperfusion was compared between groups.In addition,differences in coronary serum IL-4 and sICAM-1 levels before and 10 min after the operation were compared between groups,and the incidence rates of adverse reactions were observed.Finally,patient follow-up occurred at 1 month and 6 months,and the total incidence rates of adverse cardiac events in both groups were assessed.Results:The levels of IL-4 and sICAM-1 in the cocktail group significantly decreased after the operation(P<0.05).In addition,after the operation,significantly greater decreases in the IL-4 and sICAM-1 levels were observed in the cocktail group than the control group(P<0.05).The Seattle Angina Scale(SAQ)score of the cocktail group,compared with the control group,showed a significant improvement after vessel opening in the patients with CTO.At the 1-month follow-up,the SAQ score of the cocktail group,compared with the control group,indicated further improvements in terms of angina attack frequency.No significant differences were observed in the incidence rates of adverse reactions between groups(P>0.05).Conclusion:The treatment of patients with CTO undergoing PCI with nicorandil and tirofiban alleviated the inflam-matory response,improved the SAQ scores,and decreased the occurrence of angina pectoris in patients.Moreover,this treatment is safe and reliable,and has important clinical significance.展开更多
Background Data on retrograde recanalization of epicardial collateral channels in patients with chronic total occlusion(CTO) undergoing percutaneous coronary intervention(PCI) are limited. Fearing about the complicati...Background Data on retrograde recanalization of epicardial collateral channels in patients with chronic total occlusion(CTO) undergoing percutaneous coronary intervention(PCI) are limited. Fearing about the complications of perforation, few CTO patients have chosen retrograde recanalization of epicardial collateral channels. Methods A retrospective analysis was conducted in this study. We followed up eligible patients who underwent continuous CTO via epicardial CCS from August 2011 to October 2018 in our center, and analyzed the detailed clinical baseline and angiographic data, surgical success rate and hospital complications to determine the efficacy and safety. The Cox model was used to compare major adverse cardiac events(MACE) and related co-variables between CTO patients with epicardial CCS perforation and non-perforated PCI patients. Results The study enrolled 191 patients with epicardial CCs PCI of CTO and the long term follow-up time was 24.03±19.18 months.Successful CCs PCI of CTO was achieved in 161(84.8 %) patients. Procedural collateral perforation occurred in23(12.0%) patients. MACEs occurred in 18 patients(9.4%) at 1-year follow-up. Kaplan-Meier curves showed no significant difference in MACEs between perforating recanalization of CCs PCI of CTO and non-perforating PCI of CTO.( adjusted hazard ratios(HR): 0.467; 95% confidence intervals(CI): 0.13 to 1.69, P=0.245). The multivariate Cox regression analysis identified the Werner score=0/1(HR: 5.80; 95% CI: 1.89 to 17.8; P=0.002) as significant independent predictors of perforation of CCs PCI of CTO. Conclusions The major complication of the procedure is collateral perforation. Recanalization of CTOs through epicardial collateral channel is safe and effective. It may be a available choice for recanalization of complex CTO.[S Chin J Cardiol 2019;20(1):37-43]展开更多
Objective: to analyze the clinical effects of abdominal aorta (AA) balloon occlusion combined with UAE surgery in the treatment of dangerous placenta previa. Methods: a total of 50 patients with dangerous placenta pre...Objective: to analyze the clinical effects of abdominal aorta (AA) balloon occlusion combined with UAE surgery in the treatment of dangerous placenta previa. Methods: a total of 50 patients with dangerous placenta previa from January 2020 to December 2020 were randomly divided into two groups. The patients in the simple surgical treatment intervention group were given UAE surgical treatment, and the patients in the combined surgical treatment intervention group were given AA balloon occlusion combined with UAE surgical treatment. The therapeutic effects of the two groups were compared. Results: in the combined surgical treatment intervention group, the surgical treatment time was (108.21±39.12) minutes, the amount of bleeding was (890.12±390.12) ml and the amount of red blood cell infusion was (620.21±146.21) ml. The surgical treatment time, bleeding volume and red blood cell infusion volume in the simple surgical treatment intervention group were (108.21±39.21) minutes, (1360.12±346.12) ml and (985.21±218.12) ml respectively. Compared with the surgical treatment time in the two groups (P > 0.05), the bleeding volume and red blood cell infusion volume in the combined surgical treatment intervention group were lower (P < 0.05). The hysterectomy between the two groups was compared (P > 0.05). Conclusion: the clinical effect of AA balloon occlusion combined with UAE in the treatment of dangerous placenta previa is definite and worthy of promotion.展开更多
Objective: This work compares the occlusive effect and the penetration enhancement ability of solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC), through in vitro skin. Methods: SLN and NLC were p...Objective: This work compares the occlusive effect and the penetration enhancement ability of solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC), through in vitro skin. Methods: SLN and NLC were prepared by high shear homogenization and characterized by size, polydispersity index, zeta potential, morphology and physical stability. Occlusive effect was assessed by an in vitro test and by measuring TEWL using pig skin. Skin treated with the lipid carriers was visualized by SEM. A penetration test through skin, followed by tape stripping, was carried out using Nile red as a marker. Results: SLN (200 ± 6 nm) and NLC (192 ± 11 nm) were obtained. An occlusion factor of 36% - 39% was observed for both systems, while a reduction in TEWL of 34.3% ± 14.8% and 26.2% ± 6.5% was seen after treatment with SLN and NLC, respectively. SEM images showed a film formed by the lipid carriers, responsible for the occlusion observed. No differences were found between the occlusive effect produced by SLN and NLC in both tests. NLC allowed the penetration of a greater amount of Nile red than SLN: 4.7 ± 1.3 μg and 1.7 ± 0.4 μg, respectively. Conclusion: Both carriers form a film on the skin, providing an occlusive effect with no differences between these two systems. The penetration of a marker (Nile red) into the stratum corneum was quite higher for NLC than for SLN, suggesting an influence of the composition of these particles on their penetration enhancing ability.展开更多
mouth occlusion pressure (P<sub>0.1</sub>) rather than inspiratory ventilation （V<sub>1</sub>）and mean inspiratory airflow （（?）） was used for expounding the mechanism of indi-vidual hypox...mouth occlusion pressure (P<sub>0.1</sub>) rather than inspiratory ventilation （V<sub>1</sub>）and mean inspiratory airflow （（?）） was used for expounding the mechanism of indi-vidual hypoxic sensitivity.Eighteen young healthy male subjects participated inthe experiment of progressive isocapnic hypoxia produced by rebreathing method.The results showed that there were significantly linear relationship （P【0.01） be-tween V<sub>1</sub> and P<sub>0.1</sub>,as well as （?） and P<sub>0.1</sub> during the hypoxic loading of twoend-tidal carbon dioxide pressure (P<sub>ETCO<sub>2</sub></sub> levels,4.3 and 5.9kPa.Ventilation in-creased with progressive hypoxia.Therefore,they all represent the useful indexesof inspiratory drive.P<sub>0.1</sub> is more sensitive than V<sub>1</sub> and （?） because it isindependent of pulmonary mechanics.展开更多
Objective:To investigate the clinical effects of applying the magnetic resonance double mismatch technique to endovascular treatment of acute anterior circulation,large vessel occlusion with cerebral infarction in an ...Objective:To investigate the clinical effects of applying the magnetic resonance double mismatch technique to endovascular treatment of acute anterior circulation,large vessel occlusion with cerebral infarction in an unknown time window.Methods:The research work was carried out in our hospital,the work was carried out from November 2018 to November 2019,the patients with acute anterior circulation large vessel occlusion with cerebral infarction who were treated in our hospital during this period,100 patients,50 patients with an unknown time window and 50 patients with definite time window were selected,and they were named as the experimental and control groups,given different examination methods,were given to investigate the clinical treatment effect.Results:Patients’data on HIHSS score before treatment,the incidence of intracranial hemorrhage and rate of Mrs≤2 rating after 90 days of treatment were not significantly different(P>0.05),which was not meaningful.The differences in data between the two groups concerning HIHSS scores were relatively significant before,and after treatment(P<0.05).Conclusion:The magnetic resonance double mismatch technique will be applied in the endovascular treatment of acute anterior circulation large vessel occlusion with cerebral infarction of unknown time window.展开更多
Aim Reduction of Sheng-Nao-Kang decoction （RSNK）, is a modified traditional Chinese medicinal formula of Sheng-Nao-Kang pill preparation, which is protective in rats against focal cerebral ischemia/reperfusion （I/R...Aim Reduction of Sheng-Nao-Kang decoction （RSNK）, is a modified traditional Chinese medicinal formula of Sheng-Nao-Kang pill preparation, which is protective in rats against focal cerebral ischemia/reperfusion （I/R） injury. In the current study, we investigate the protective effect of RSNK against apoptosis and oxidative damage induced by cerebral I/R and explore the underlying mechanisms. Cerebral I/R injury was induced by in- traluminal middle cerebral artery occlusion （MCAO） for 2 h followed by reperfusion for 24 h in adult male Sprague- Dawley rats. Rats were randomized into seven groups （n- 8）： Sham group, I/R group, RSNK-treated groups （ 0.7 g · kg ^- 1, 1 . 4 g · kg ^- 1 and 2. 8 g · kg^ - 1 ） , nimodipine （NMP） -treated group and Whitmania pigra Whitman （WW）-treated group. Neurological deficit scores, cerebral humidity content and cerebral infarction volume were measured after the 24 h reperfusion. Malondialdehyde （ MDA）, superoxide dismutase （ SOD）, catalase （ CAT）, inducible nitric oxide synthase （iNOS） and total nitric oxide synthase （TNOS） in serum were measured by assay kits for biochemical analysis. Histological structures of the cortex of the ipsilateral ischemic cerebral hemisphere in rats were observed by Nissl staining. The caspase-3 protein content in the hippocampus and cortex was detected by immunohistochemistry. Additionally, Bax and Bcl-2 protein expressions in the injured brain were evaluated by Western blot. RSNK administration not only markedly improved neurological deficit scores, but also reduced cere- bral humidity content and cerebral infarction volume, lowered MDA content, up-regulated SOD and CAT levels, down-regulated iNOS and TNOS levels, restrained the expression of caspase-3 positive protein and alleviated the Bax and Bcl-2 protein expressions.展开更多
Objective： To investigate the influence of acupuncture parameters on the acupuncture effect through the cluster analysis in Wistar rat model with middle cerebral artery occlusion （MCAO）. Methods： Duplicated MCAO m...Objective： To investigate the influence of acupuncture parameters on the acupuncture effect through the cluster analysis in Wistar rat model with middle cerebral artery occlusion （MCAO）. Methods： Duplicated MCAO models by Zea-longa＇s thread ligation and chose rats with 1-3 scores assessed by Zausinger＇s six-score method to be grouped. The rats were divided into 6 basic control groups [（including a normal group, a sham group, a model control group, a model group without intervention, a Nimodipine group, a lateral-to-Renzhong （DU6） group] and 6 acupuncture groups [a Neiguan （PC6） group, a Weizhong （BL40） group, a Sanyinjiao （SP6） group, a Chize （LU5） group, a Renzhong （DU6） group and a Feixue （non-acupoint） group]. In the acupuncture groups, for every acupoint or needling site, 9 different parameters [2 factors （frequency and time） and 3 levels （180, 120, and 60 cpm of the frequency and 5, 60, and 180 s of the time）] were set respectively by the orthogonal intersection method, in total 54 groups. The rats were treated by acupuncture with a lifting-thrusting manipulation once every 12 h, in total 6 times. Neurobehavioral scores, cerebral blood flow, infarction rate, microcirculation, light microscopy, etc. were measured. The factor analysis was first applied to get the comprehensive effect scores of the samples in the acupuncture groups and then by which the cluster analysis was made with the statistical software of SPSS17.0. Results： For the Neiguan （PC6） group, the exceptional results of acupuncture comprehensive effect were parameters 7, 8, 9, 10; the valid results were parameters 2, 3, 4, and the invalid were parameters 5, 6. For the Weizhong （BL40） group, the exceptional results were parameters 2, 4; the valid results were parameters 3, 5, 6, 7, and the invalid were parameters 8, 9, 10. For the Chize （LU5） group, the exceptional results were parameters 7, 8; the valid results were parameters 3, 4, 5, 6, 9, 10; and the invalid was parameter 2. For the Sanyinjiao （SP6） group, the exceptional results were parameters 4, 6; the valid results were parameters 2, 3, 5; and the invalid were parameters 7, 8, 9,10. For the Renzhong （DU6） group, the exceptional results were parameters 3, 4, 6, 7, 9, 10; the valid results were parameters 2, 5; and the invalid was parameter 8. For the Non-acupoint group, the exceptional result was parameter 10; the valid results were parameters 2, 3, 4, 7, 9; and the invalid were parameters 5, 6, 8. Conclusions： For each meridian acupoint, different acupuncture parameters could consequently get a different acupuncture effect; each meridian acupoint had the most suitable or optimal acupuncture parameters; acupuncture parameters might be the main factors impacting on acupuncture effect.展开更多
基金supported by research grants from the Natural Science Foundation of Shandong Province[grant number ZR2017BH114],the National Natural Science Foundation of China[grant number 81700334],and Jinan Science and Technology Plan Project[grant number 201805058].
文摘Aim:The effects of tirofiban combined with nicorandil on effective reperfusion,and the levels of interleukin-4(IL-4)and soluble intercellular adhesion molecule-1(sICAM-1)after percutaneous coronary intervention(PCI)for chronic coronary total occlusion(CTO)were investigated.Method:From January 1,2017,to June 31,2019,a total of 40 patients with CTO receiving PCI in Shandong Qian-foshan Hospital were randomly divided into a control group(treated with single tirofiban)and a cocktail group(treated with nicorandil combined with tirofiban).Effective reperfusion was compared between groups.In addition,differences in coronary serum IL-4 and sICAM-1 levels before and 10 min after the operation were compared between groups,and the incidence rates of adverse reactions were observed.Finally,patient follow-up occurred at 1 month and 6 months,and the total incidence rates of adverse cardiac events in both groups were assessed.Results:The levels of IL-4 and sICAM-1 in the cocktail group significantly decreased after the operation(P<0.05).In addition,after the operation,significantly greater decreases in the IL-4 and sICAM-1 levels were observed in the cocktail group than the control group(P<0.05).The Seattle Angina Scale(SAQ)score of the cocktail group,compared with the control group,showed a significant improvement after vessel opening in the patients with CTO.At the 1-month follow-up,the SAQ score of the cocktail group,compared with the control group,indicated further improvements in terms of angina attack frequency.No significant differences were observed in the incidence rates of adverse reactions between groups(P>0.05).Conclusion:The treatment of patients with CTO undergoing PCI with nicorandil and tirofiban alleviated the inflam-matory response,improved the SAQ scores,and decreased the occurrence of angina pectoris in patients.Moreover,this treatment is safe and reliable,and has important clinical significance.
基金supported by Science and Technology Planning Project of Guangdong Province(No.2016A020216022)
文摘Background Data on retrograde recanalization of epicardial collateral channels in patients with chronic total occlusion(CTO) undergoing percutaneous coronary intervention(PCI) are limited. Fearing about the complications of perforation, few CTO patients have chosen retrograde recanalization of epicardial collateral channels. Methods A retrospective analysis was conducted in this study. We followed up eligible patients who underwent continuous CTO via epicardial CCS from August 2011 to October 2018 in our center, and analyzed the detailed clinical baseline and angiographic data, surgical success rate and hospital complications to determine the efficacy and safety. The Cox model was used to compare major adverse cardiac events(MACE) and related co-variables between CTO patients with epicardial CCS perforation and non-perforated PCI patients. Results The study enrolled 191 patients with epicardial CCs PCI of CTO and the long term follow-up time was 24.03±19.18 months.Successful CCs PCI of CTO was achieved in 161(84.8 %) patients. Procedural collateral perforation occurred in23(12.0%) patients. MACEs occurred in 18 patients(9.4%) at 1-year follow-up. Kaplan-Meier curves showed no significant difference in MACEs between perforating recanalization of CCs PCI of CTO and non-perforating PCI of CTO.( adjusted hazard ratios(HR): 0.467; 95% confidence intervals(CI): 0.13 to 1.69, P=0.245). The multivariate Cox regression analysis identified the Werner score=0/1(HR: 5.80; 95% CI: 1.89 to 17.8; P=0.002) as significant independent predictors of perforation of CCs PCI of CTO. Conclusions The major complication of the procedure is collateral perforation. Recanalization of CTOs through epicardial collateral channel is safe and effective. It may be a available choice for recanalization of complex CTO.[S Chin J Cardiol 2019;20(1):37-43]
文摘Objective: to analyze the clinical effects of abdominal aorta (AA) balloon occlusion combined with UAE surgery in the treatment of dangerous placenta previa. Methods: a total of 50 patients with dangerous placenta previa from January 2020 to December 2020 were randomly divided into two groups. The patients in the simple surgical treatment intervention group were given UAE surgical treatment, and the patients in the combined surgical treatment intervention group were given AA balloon occlusion combined with UAE surgical treatment. The therapeutic effects of the two groups were compared. Results: in the combined surgical treatment intervention group, the surgical treatment time was (108.21±39.12) minutes, the amount of bleeding was (890.12±390.12) ml and the amount of red blood cell infusion was (620.21±146.21) ml. The surgical treatment time, bleeding volume and red blood cell infusion volume in the simple surgical treatment intervention group were (108.21±39.21) minutes, (1360.12±346.12) ml and (985.21±218.12) ml respectively. Compared with the surgical treatment time in the two groups (P > 0.05), the bleeding volume and red blood cell infusion volume in the combined surgical treatment intervention group were lower (P < 0.05). The hysterectomy between the two groups was compared (P > 0.05). Conclusion: the clinical effect of AA balloon occlusion combined with UAE in the treatment of dangerous placenta previa is definite and worthy of promotion.
文摘Objective: This work compares the occlusive effect and the penetration enhancement ability of solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC), through in vitro skin. Methods: SLN and NLC were prepared by high shear homogenization and characterized by size, polydispersity index, zeta potential, morphology and physical stability. Occlusive effect was assessed by an in vitro test and by measuring TEWL using pig skin. Skin treated with the lipid carriers was visualized by SEM. A penetration test through skin, followed by tape stripping, was carried out using Nile red as a marker. Results: SLN (200 ± 6 nm) and NLC (192 ± 11 nm) were obtained. An occlusion factor of 36% - 39% was observed for both systems, while a reduction in TEWL of 34.3% ± 14.8% and 26.2% ± 6.5% was seen after treatment with SLN and NLC, respectively. SEM images showed a film formed by the lipid carriers, responsible for the occlusion observed. No differences were found between the occlusive effect produced by SLN and NLC in both tests. NLC allowed the penetration of a greater amount of Nile red than SLN: 4.7 ± 1.3 μg and 1.7 ± 0.4 μg, respectively. Conclusion: Both carriers form a film on the skin, providing an occlusive effect with no differences between these two systems. The penetration of a marker (Nile red) into the stratum corneum was quite higher for NLC than for SLN, suggesting an influence of the composition of these particles on their penetration enhancing ability.
文摘mouth occlusion pressure (P<sub>0.1</sub>) rather than inspiratory ventilation （V<sub>1</sub>）and mean inspiratory airflow （（?）） was used for expounding the mechanism of indi-vidual hypoxic sensitivity.Eighteen young healthy male subjects participated inthe experiment of progressive isocapnic hypoxia produced by rebreathing method.The results showed that there were significantly linear relationship （P【0.01） be-tween V<sub>1</sub> and P<sub>0.1</sub>,as well as （?） and P<sub>0.1</sub> during the hypoxic loading of twoend-tidal carbon dioxide pressure (P<sub>ETCO<sub>2</sub></sub> levels,4.3 and 5.9kPa.Ventilation in-creased with progressive hypoxia.Therefore,they all represent the useful indexesof inspiratory drive.P<sub>0.1</sub> is more sensitive than V<sub>1</sub> and （?） because it isindependent of pulmonary mechanics.
文摘Objective:To investigate the clinical effects of applying the magnetic resonance double mismatch technique to endovascular treatment of acute anterior circulation,large vessel occlusion with cerebral infarction in an unknown time window.Methods:The research work was carried out in our hospital,the work was carried out from November 2018 to November 2019,the patients with acute anterior circulation large vessel occlusion with cerebral infarction who were treated in our hospital during this period,100 patients,50 patients with an unknown time window and 50 patients with definite time window were selected,and they were named as the experimental and control groups,given different examination methods,were given to investigate the clinical treatment effect.Results:Patients’data on HIHSS score before treatment,the incidence of intracranial hemorrhage and rate of Mrs≤2 rating after 90 days of treatment were not significantly different(P>0.05),which was not meaningful.The differences in data between the two groups concerning HIHSS scores were relatively significant before,and after treatment(P<0.05).Conclusion:The magnetic resonance double mismatch technique will be applied in the endovascular treatment of acute anterior circulation large vessel occlusion with cerebral infarction of unknown time window.
文摘Aim Reduction of Sheng-Nao-Kang decoction （RSNK）, is a modified traditional Chinese medicinal formula of Sheng-Nao-Kang pill preparation, which is protective in rats against focal cerebral ischemia/reperfusion （I/R） injury. In the current study, we investigate the protective effect of RSNK against apoptosis and oxidative damage induced by cerebral I/R and explore the underlying mechanisms. Cerebral I/R injury was induced by in- traluminal middle cerebral artery occlusion （MCAO） for 2 h followed by reperfusion for 24 h in adult male Sprague- Dawley rats. Rats were randomized into seven groups （n- 8）： Sham group, I/R group, RSNK-treated groups （ 0.7 g · kg ^- 1, 1 . 4 g · kg ^- 1 and 2. 8 g · kg^ - 1 ） , nimodipine （NMP） -treated group and Whitmania pigra Whitman （WW）-treated group. Neurological deficit scores, cerebral humidity content and cerebral infarction volume were measured after the 24 h reperfusion. Malondialdehyde （ MDA）, superoxide dismutase （ SOD）, catalase （ CAT）, inducible nitric oxide synthase （iNOS） and total nitric oxide synthase （TNOS） in serum were measured by assay kits for biochemical analysis. Histological structures of the cortex of the ipsilateral ischemic cerebral hemisphere in rats were observed by Nissl staining. The caspase-3 protein content in the hippocampus and cortex was detected by immunohistochemistry. Additionally, Bax and Bcl-2 protein expressions in the injured brain were evaluated by Western blot. RSNK administration not only markedly improved neurological deficit scores, but also reduced cere- bral humidity content and cerebral infarction volume, lowered MDA content, up-regulated SOD and CAT levels, down-regulated iNOS and TNOS levels, restrained the expression of caspase-3 positive protein and alleviated the Bax and Bcl-2 protein expressions.
基金Supported by the Development Plan of National Critical and Basic Research(No.2012CB518505,2010CB530500,and 2006CB504504)
文摘Objective： To investigate the influence of acupuncture parameters on the acupuncture effect through the cluster analysis in Wistar rat model with middle cerebral artery occlusion （MCAO）. Methods： Duplicated MCAO models by Zea-longa＇s thread ligation and chose rats with 1-3 scores assessed by Zausinger＇s six-score method to be grouped. The rats were divided into 6 basic control groups [（including a normal group, a sham group, a model control group, a model group without intervention, a Nimodipine group, a lateral-to-Renzhong （DU6） group] and 6 acupuncture groups [a Neiguan （PC6） group, a Weizhong （BL40） group, a Sanyinjiao （SP6） group, a Chize （LU5） group, a Renzhong （DU6） group and a Feixue （non-acupoint） group]. In the acupuncture groups, for every acupoint or needling site, 9 different parameters [2 factors （frequency and time） and 3 levels （180, 120, and 60 cpm of the frequency and 5, 60, and 180 s of the time）] were set respectively by the orthogonal intersection method, in total 54 groups. The rats were treated by acupuncture with a lifting-thrusting manipulation once every 12 h, in total 6 times. Neurobehavioral scores, cerebral blood flow, infarction rate, microcirculation, light microscopy, etc. were measured. The factor analysis was first applied to get the comprehensive effect scores of the samples in the acupuncture groups and then by which the cluster analysis was made with the statistical software of SPSS17.0. Results： For the Neiguan （PC6） group, the exceptional results of acupuncture comprehensive effect were parameters 7, 8, 9, 10; the valid results were parameters 2, 3, 4, and the invalid were parameters 5, 6. For the Weizhong （BL40） group, the exceptional results were parameters 2, 4; the valid results were parameters 3, 5, 6, 7, and the invalid were parameters 8, 9, 10. For the Chize （LU5） group, the exceptional results were parameters 7, 8; the valid results were parameters 3, 4, 5, 6, 9, 10; and the invalid was parameter 2. For the Sanyinjiao （SP6） group, the exceptional results were parameters 4, 6; the valid results were parameters 2, 3, 5; and the invalid were parameters 7, 8, 9,10. For the Renzhong （DU6） group, the exceptional results were parameters 3, 4, 6, 7, 9, 10; the valid results were parameters 2, 5; and the invalid was parameter 8. For the Non-acupoint group, the exceptional result was parameter 10; the valid results were parameters 2, 3, 4, 7, 9; and the invalid were parameters 5, 6, 8. Conclusions： For each meridian acupoint, different acupuncture parameters could consequently get a different acupuncture effect; each meridian acupoint had the most suitable or optimal acupuncture parameters; acupuncture parameters might be the main factors impacting on acupuncture effect.
