Droplet microfluidics enable high-throughput screening,sequencing,and formulation of biological and chemical systems at the microscale.Such devices are generally fabricated in a soft polymer such as polydimethylsiloxa...Droplet microfluidics enable high-throughput screening,sequencing,and formulation of biological and chemical systems at the microscale.Such devices are generally fabricated in a soft polymer such as polydimethylsiloxane(PDMS).However,developing design masks for PDMS devices can be a slow and expensive process,requiring an internal cleanroom facility or using an external vendor.Here,we present the first complete droplet-based component library using low-cost rapid prototyping and electrode integration.This fabrication method for droplet microfluidic devices costs less than$12 per device and a full design-build-test cycle can be completed within a day.Discrete microfluidic components for droplet generation,re-injection,picoinjection,anchoring,fluorescence sensing,and sorting were built and characterized.These devices are biocompatible,low-cost,and high-throughput.To show its ability to perform multistep workflows,these components were used to assemble droplet"pixel"arrays,where droplets were generated,sensed,sorted,and anchored onto a grid to produce images.展开更多
基金supported by NSF Semiconductor Synthetic Biology for Information Storage and Retrieval(Award#2027045)。
文摘Droplet microfluidics enable high-throughput screening,sequencing,and formulation of biological and chemical systems at the microscale.Such devices are generally fabricated in a soft polymer such as polydimethylsiloxane(PDMS).However,developing design masks for PDMS devices can be a slow and expensive process,requiring an internal cleanroom facility or using an external vendor.Here,we present the first complete droplet-based component library using low-cost rapid prototyping and electrode integration.This fabrication method for droplet microfluidic devices costs less than$12 per device and a full design-build-test cycle can be completed within a day.Discrete microfluidic components for droplet generation,re-injection,picoinjection,anchoring,fluorescence sensing,and sorting were built and characterized.These devices are biocompatible,low-cost,and high-throughput.To show its ability to perform multistep workflows,these components were used to assemble droplet"pixel"arrays,where droplets were generated,sensed,sorted,and anchored onto a grid to produce images.
