AIM: To investigate the chemical and bioactive constituents from the stems and leaves of Micromelum integerrimum. METHOD: The chemical constituents were isolated and purified by silica gel, Sephadex LH-20, and HPLC. T...AIM: To investigate the chemical and bioactive constituents from the stems and leaves of Micromelum integerrimum. METHOD: The chemical constituents were isolated and purified by silica gel, Sephadex LH-20, and HPLC. Their structures were mainly elucidated on the basis of extensive 1D- and 2D-NMR spectroscopy and mass spectrometry. Their cytotoxicity and antimicrobial activities were tested by the SRB and turbidimetric methods, respectively. RESULTS: Two new phenylpropanoids and two known coumarins were obtained, and their structures were identified as microintegerrin A(1), microintegerrin B(2), scopoletin(3), and scopolin(4). All of the compounds were tested for their cytotoxicity against three cancer cell lines(HeLa, A549, and BGC-823) and for antimicrobial activity against the fungus Candida albicans and the bacterium Staphylococcus aureus. CONCLUSION: Two new phenylpropanoids 1 and 2 were isolated and identified from the stems and leaves of M. intgerrimum. None of the compounds showed cytotoxic or antimicrobial activity at the tested concentration of 20 ?g·mL–1.展开更多
Three derivatives of micromelin, named hydramicromelins A-C (1-3), were isolated from the aerial part of Micromelum integerrimum (Buch.-Ham) Roem., respectively. Hydramicromelins A-C (1-3) were epimers which possessed...Three derivatives of micromelin, named hydramicromelins A-C (1-3), were isolated from the aerial part of Micromelum integerrimum (Buch.-Ham) Roem., respectively. Hydramicromelins A-C (1-3) were epimers which possessed the same plane structure and molecular formula. Their structures were elucidated based on MS and NMR data. The relative configurations of 1-3 were established by NOE analysis.展开更多
Three new coumarins,integmarins A–C(1–3),and a new coumarin glycoside,integmaside A(4)were isolated from the leaves and stems of Micromelum integerrimum.Their structures were elucidated on the basis of 1 D and 2 D N...Three new coumarins,integmarins A–C(1–3),and a new coumarin glycoside,integmaside A(4)were isolated from the leaves and stems of Micromelum integerrimum.Their structures were elucidated on the basis of 1 D and 2 D NMR and MS data,and their absolute configurations were assigned according to the ECD data of the in situ formed transition metal complexes and comparison of experimental and calculated ECD data.Compounds 1 and 2 are two rare coumarins with butyl and propyl moieties at the C-6 position;compound 3 is a novel coumarin with a highly oxidized prenyl group,and compound 4 is a rare bisdihydrofuranocoumarin glycoside.展开更多
在黑暗条件下对全缘马尾藻（Sargassum integerrimum）幼孢子分别进行0.5、1、2、4 h 干出处理,研究干出处理对幼孢子体生长、幼孢子体体内叶绿素a、叶绿素c、类胡萝卜素、脯氨酸以及可溶性糖含量的影响.结果表明：相比于未进行干出处...在黑暗条件下对全缘马尾藻（Sargassum integerrimum）幼孢子分别进行0.5、1、2、4 h 干出处理,研究干出处理对幼孢子体生长、幼孢子体体内叶绿素a、叶绿素c、类胡萝卜素、脯氨酸以及可溶性糖含量的影响.结果表明：相比于未进行干出处理的对照组,0.5 h、1 h 组的短暂干出可促进幼孢子体生长（P〈0.05）,幼孢子体体内两种叶绿素、类胡萝卜素、脯氨酸以及可溶性糖含量差异均无统计学意义（P〉0.05）;2 h、4 h组的长时间干出可抑制幼孢子体生长（P〈0.05）,幼孢子体体内两种叶绿素含量均降低（P〈0.05）,类胡萝卜素、脯氨酸以及可溶性糖的含量升高（P〈0.05）并保持较高水平.在人工育苗过程中每天在黑暗条件下对幼孢子体进行短时间（0.5～1 h）干出处理,可促进幼孢子体生长,并适当提高其抗逆性以及对干旱的适应性.展开更多
基金supported by the National Natural Science Foundation of China(Nos.81001379,U1032602,91013002 and 30725048)the National New Drug Innovation Major Project of China(No.2011ZX09307-002-02)+3 种基金the National Basic Research Program of China(Nos.2009CB522300 and 2013CB127505)the Foundation of Chinese Academy of Sciences(Hundred Talents Program)the Natural Science Foundation of Yunnan Province(No.2012GA 003)the Project Program of State Key Laboratory of Natural Medicines,China Pharmaceutical University(No.ZJ11175)
文摘AIM: To investigate the chemical and bioactive constituents from the stems and leaves of Micromelum integerrimum. METHOD: The chemical constituents were isolated and purified by silica gel, Sephadex LH-20, and HPLC. Their structures were mainly elucidated on the basis of extensive 1D- and 2D-NMR spectroscopy and mass spectrometry. Their cytotoxicity and antimicrobial activities were tested by the SRB and turbidimetric methods, respectively. RESULTS: Two new phenylpropanoids and two known coumarins were obtained, and their structures were identified as microintegerrin A(1), microintegerrin B(2), scopoletin(3), and scopolin(4). All of the compounds were tested for their cytotoxicity against three cancer cell lines(HeLa, A549, and BGC-823) and for antimicrobial activity against the fungus Candida albicans and the bacterium Staphylococcus aureus. CONCLUSION: Two new phenylpropanoids 1 and 2 were isolated and identified from the stems and leaves of M. intgerrimum. None of the compounds showed cytotoxic or antimicrobial activity at the tested concentration of 20 ?g·mL–1.
基金This work was financially supported by a grant from the National Nature Science Foundation of China for Outstanding Young Scientists to Dr.X.J.Hao(No.39525025).
文摘Three derivatives of micromelin, named hydramicromelins A-C (1-3), were isolated from the aerial part of Micromelum integerrimum (Buch.-Ham) Roem., respectively. Hydramicromelins A-C (1-3) were epimers which possessed the same plane structure and molecular formula. Their structures were elucidated based on MS and NMR data. The relative configurations of 1-3 were established by NOE analysis.
基金supported by the National Natural Sciences Foundation of China(Nos.81973199,81773864 and81473106)the Drug Innovation Major Project of China(No.2018ZX09711001-008-003)。
文摘Three new coumarins,integmarins A–C(1–3),and a new coumarin glycoside,integmaside A(4)were isolated from the leaves and stems of Micromelum integerrimum.Their structures were elucidated on the basis of 1 D and 2 D NMR and MS data,and their absolute configurations were assigned according to the ECD data of the in situ formed transition metal complexes and comparison of experimental and calculated ECD data.Compounds 1 and 2 are two rare coumarins with butyl and propyl moieties at the C-6 position;compound 3 is a novel coumarin with a highly oxidized prenyl group,and compound 4 is a rare bisdihydrofuranocoumarin glycoside.
基金Project supported by the National Natural Science Foundation of China(31470428,21202147,81001379)National New Drug Innovation Major Project of China(2011ZX09307-002-02)Natural Science Foundation of Yunnan Province(2012GA003)
文摘在黑暗条件下对全缘马尾藻（Sargassum integerrimum）幼孢子分别进行0.5、1、2、4 h 干出处理,研究干出处理对幼孢子体生长、幼孢子体体内叶绿素a、叶绿素c、类胡萝卜素、脯氨酸以及可溶性糖含量的影响.结果表明：相比于未进行干出处理的对照组,0.5 h、1 h 组的短暂干出可促进幼孢子体生长（P〈0.05）,幼孢子体体内两种叶绿素、类胡萝卜素、脯氨酸以及可溶性糖含量差异均无统计学意义（P〉0.05）;2 h、4 h组的长时间干出可抑制幼孢子体生长（P〈0.05）,幼孢子体体内两种叶绿素含量均降低（P〈0.05）,类胡萝卜素、脯氨酸以及可溶性糖的含量升高（P〈0.05）并保持较高水平.在人工育苗过程中每天在黑暗条件下对幼孢子体进行短时间（0.5～1 h）干出处理,可促进幼孢子体生长,并适当提高其抗逆性以及对干旱的适应性.
