Objective:To carry out an experimental study with the main objective of mass rearing of sheep flies(Lucilia sericata).Methods:Hand collection and beef- or cattle liver-baited net traps were used for field fly sampling...Objective:To carry out an experimental study with the main objective of mass rearing of sheep flies(Lucilia sericata).Methods:Hand collection and beef- or cattle liver-baited net traps were used for field fly sampling from April,2010 to November,2010.The samples collected from different places were placed in properly labeled tubes and sent to the Entomology Laboratory. Since maggot identification is important in inducing mortality,they were kept under insectary condition to develop to adult stage and identified using systematic keys.Results:A total of 218 flies were collected in three rounds of sampling from the field of Tehran and Karaj Counties.In the first generation,433 flies including 135(31.17%) male,and 298(68.82%) female were yielded. The female/male of parent ratio was calculated as 1.72 in Tehran and in Karaj areas,whereas it was 2,20%and 1.81%,respectively in Fl and F2 generations,respectively.Conclusions:During this study,the mass rearing of sheep blow fly has been established at the School of Public Health, Tehran University of Medical Sciences and can be used for producing flies for maggot therapy.展开更多
Objective:To determine the sensitivity of Blattela germanica L(B.germanica L) to differenct doses of insulin.Methods:B.germanica were reared in laboratory conditions at(25±2) ℃ and(50±5)%relative humidity(R...Objective:To determine the sensitivity of Blattela germanica L(B.germanica L) to differenct doses of insulin.Methods:B.germanica were reared in laboratory conditions at(25±2) ℃ and(50±5)%relative humidity(RH),and exposure period of 12:12 L/D.Different concentrations,viz.5,10,15,20 and 25 μ of insulin N,R,(N+R) were prepared and injected to 10 treated cockroaches with another 10 cockroaches which were injected with normal saline as control group.Results:Insulin N with a dose of 20 μ caused more than 70%mortality of B.germanica in this study.There was a significant difference between 20 μ of insulin N with other doses of 5,10,15 and 25 μ,and its comparison with other forms of medication also showed obvious difference(P<0.05).Conclusions:It can be concluded that effective drug doses of insulin which can be used as posion bait or gel against German cockroaches could be utilized in the control of B.germanica in the future field studies.展开更多
Tsetse flies are the sole cyclic vectors of African trypanosomes,which cause human and animal African trypanosomiases in Africa.Tsetse fly control remains a promising option for disease management.The sterile insect t...Tsetse flies are the sole cyclic vectors of African trypanosomes,which cause human and animal African trypanosomiases in Africa.Tsetse fly control remains a promising option for disease management.The sterile insect technique(SIT)stands as an environmentally friendly tool to control tsetse populations.SIT requires the mass-rearing of competent sterile males to mate with wild females.However,long-term colonization might affect the genetic structure of the reared flies.This study investigated the genetic structure of four Glossina palpalis gambiensis colonies of different ages:two originating from Senegal(SEN and ICIRSEN)and two from Burkina Faso(CIR and IBD).Samples from these colonies were genotyped at ten microsatellite loci,followed by downstream population genetic analyses.The results show that the two colonies from Burkina Faso collected from close sites(~20 km apart)over 45-year interval retained the same genetic background(FsT cIR~IBD≈0,P-value=0.47).These flies were however,genetically different from those from the Senegal colonies(FsT cIR~SEN≈0.047;FsT IBD~SEN≈0.058,P-value=10-4).Moreover,no significant difference was detected in the gene diversity of the CIR and IBD colonies,with Hs values of 0.650 and 0.665,respectively.Theinbreeding coefficient showed that all four colonies where under Hardy—Weinberg equilibrium,with FIs values of 0.026,0.012,-0.064,and 0.001,for CIR,IBD,ICIRSEN,and SEN.respectively.Furthermore,no sign of a recent bottleneck was identified in tsetse samples from any of the four colonies.The results suggest that long-term mass-rearing of tsetse flies has no significant impact on their genetic background and diversity.展开更多
文摘Objective:To carry out an experimental study with the main objective of mass rearing of sheep flies(Lucilia sericata).Methods:Hand collection and beef- or cattle liver-baited net traps were used for field fly sampling from April,2010 to November,2010.The samples collected from different places were placed in properly labeled tubes and sent to the Entomology Laboratory. Since maggot identification is important in inducing mortality,they were kept under insectary condition to develop to adult stage and identified using systematic keys.Results:A total of 218 flies were collected in three rounds of sampling from the field of Tehran and Karaj Counties.In the first generation,433 flies including 135(31.17%) male,and 298(68.82%) female were yielded. The female/male of parent ratio was calculated as 1.72 in Tehran and in Karaj areas,whereas it was 2,20%and 1.81%,respectively in Fl and F2 generations,respectively.Conclusions:During this study,the mass rearing of sheep blow fly has been established at the School of Public Health, Tehran University of Medical Sciences and can be used for producing flies for maggot therapy.
基金Supported by Tehran University of Medical Sciences(No:9855-2704-88)
文摘Objective:To determine the sensitivity of Blattela germanica L(B.germanica L) to differenct doses of insulin.Methods:B.germanica were reared in laboratory conditions at(25±2) ℃ and(50±5)%relative humidity(RH),and exposure period of 12:12 L/D.Different concentrations,viz.5,10,15,20 and 25 μ of insulin N,R,(N+R) were prepared and injected to 10 treated cockroaches with another 10 cockroaches which were injected with normal saline as control group.Results:Insulin N with a dose of 20 μ caused more than 70%mortality of B.germanica in this study.There was a significant difference between 20 μ of insulin N with other doses of 5,10,15 and 25 μ,and its comparison with other forms of medication also showed obvious difference(P<0.05).Conclusions:It can be concluded that effective drug doses of insulin which can be used as posion bait or gel against German cockroaches could be utilized in the control of B.germanica in the future field studies.
基金funded by the Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture,IAEA(CRP No.:D4.20.17),Vienna,Austria.
文摘Tsetse flies are the sole cyclic vectors of African trypanosomes,which cause human and animal African trypanosomiases in Africa.Tsetse fly control remains a promising option for disease management.The sterile insect technique(SIT)stands as an environmentally friendly tool to control tsetse populations.SIT requires the mass-rearing of competent sterile males to mate with wild females.However,long-term colonization might affect the genetic structure of the reared flies.This study investigated the genetic structure of four Glossina palpalis gambiensis colonies of different ages:two originating from Senegal(SEN and ICIRSEN)and two from Burkina Faso(CIR and IBD).Samples from these colonies were genotyped at ten microsatellite loci,followed by downstream population genetic analyses.The results show that the two colonies from Burkina Faso collected from close sites(~20 km apart)over 45-year interval retained the same genetic background(FsT cIR~IBD≈0,P-value=0.47).These flies were however,genetically different from those from the Senegal colonies(FsT cIR~SEN≈0.047;FsT IBD~SEN≈0.058,P-value=10-4).Moreover,no significant difference was detected in the gene diversity of the CIR and IBD colonies,with Hs values of 0.650 and 0.665,respectively.Theinbreeding coefficient showed that all four colonies where under Hardy—Weinberg equilibrium,with FIs values of 0.026,0.012,-0.064,and 0.001,for CIR,IBD,ICIRSEN,and SEN.respectively.Furthermore,no sign of a recent bottleneck was identified in tsetse samples from any of the four colonies.The results suggest that long-term mass-rearing of tsetse flies has no significant impact on their genetic background and diversity.
