Hypocotyl segments from aseptic seedlings of two important cultivars of upland cotton ( Gossypium hirsutum L.) in Northwest China, 'Xinluzao_1', 'Jinmian_7', 'Jinmian_12' and 'Jihe_321&#...Hypocotyl segments from aseptic seedlings of two important cultivars of upland cotton ( Gossypium hirsutum L.) in Northwest China, 'Xinluzao_1', 'Jinmian_7', 'Jinmian_12' and 'Jihe_321' were transformed respectively by two efficient plant expression plasmids pBinMoBc and pBinoBc via Agrobacterium tumefaciens . In pBinMoBc, cry 1Ac3 gene, which encodes the Bt toxin, is under the control of chimeric OM promoter. In pBinoBc, it is under control of CaMV 35S promoter. After co_cultivation with Agrobacterium tumefimpfaciens LBA4404 (containing pBinMoBc or pBinoBc), kanamycin_resistant selection, somatic embryos were induced and regenerated plants were obtained. Then the regenerated plantlets were grafted to untransformed stocks in greenhouse to produce descendants. The integration of cry 1Ac3 gene and its expression in T 2 generation of transgenic cotton plants were confirmed by Southern hybridization and Western blotting. The analyses of insect bioassay indicated that the transgenic plants of both constructions have significant resistance to the larvae of cotton bollworm ( Heliothis armigera ) and that cry 1Ac3 gene driven by chimeric OM promoter could endue T 2 generation cotton with high pest_resistant ability, implicating that it has a profound application in genetic engineering to breed new pest_resistant cotton varieties.展开更多
The quality and yield of crops are badly affected with direct or oblique damage by pests like cotton bollworm,aphid and the pathogens carring with them,which causes about hundreds of billion dollars losses each year i...The quality and yield of crops are badly affected with direct or oblique damage by pests like cotton bollworm,aphid and the pathogens carring with them,which causes about hundreds of billion dollars losses each year in agri-culture worldwide(Estruch et al,1997).The widely use of chemical pesticides inevitably brings serious safety and pollution problems.In order to effectively and reasonably control agronomically important ous and homopterous pests,Bt(Vaeck et al,1987),CpT1(Hilder et al,1987)and GNA(Hilder et al,1995)genes have been applied in crop breeding through genetic engineering tech-niques.Tolerance of pests to insect-resistant transgenic plants would evolve if these kinds of transgenic plants are cultivated in a large scale and long duration.And the procedure for screening of transgenic plants is so tedious and time-consuming as to slow down the progress of crop breeding for insect-resistance.展开更多
文摘Hypocotyl segments from aseptic seedlings of two important cultivars of upland cotton ( Gossypium hirsutum L.) in Northwest China, 'Xinluzao_1', 'Jinmian_7', 'Jinmian_12' and 'Jihe_321' were transformed respectively by two efficient plant expression plasmids pBinMoBc and pBinoBc via Agrobacterium tumefaciens . In pBinMoBc, cry 1Ac3 gene, which encodes the Bt toxin, is under the control of chimeric OM promoter. In pBinoBc, it is under control of CaMV 35S promoter. After co_cultivation with Agrobacterium tumefimpfaciens LBA4404 (containing pBinMoBc or pBinoBc), kanamycin_resistant selection, somatic embryos were induced and regenerated plants were obtained. Then the regenerated plantlets were grafted to untransformed stocks in greenhouse to produce descendants. The integration of cry 1Ac3 gene and its expression in T 2 generation of transgenic cotton plants were confirmed by Southern hybridization and Western blotting. The analyses of insect bioassay indicated that the transgenic plants of both constructions have significant resistance to the larvae of cotton bollworm ( Heliothis armigera ) and that cry 1Ac3 gene driven by chimeric OM promoter could endue T 2 generation cotton with high pest_resistant ability, implicating that it has a profound application in genetic engineering to breed new pest_resistant cotton varieties.
文摘The quality and yield of crops are badly affected with direct or oblique damage by pests like cotton bollworm,aphid and the pathogens carring with them,which causes about hundreds of billion dollars losses each year in agri-culture worldwide(Estruch et al,1997).The widely use of chemical pesticides inevitably brings serious safety and pollution problems.In order to effectively and reasonably control agronomically important ous and homopterous pests,Bt(Vaeck et al,1987),CpT1(Hilder et al,1987)and GNA(Hilder et al,1995)genes have been applied in crop breeding through genetic engineering tech-niques.Tolerance of pests to insect-resistant transgenic plants would evolve if these kinds of transgenic plants are cultivated in a large scale and long duration.And the procedure for screening of transgenic plants is so tedious and time-consuming as to slow down the progress of crop breeding for insect-resistance.
