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Wound Healing Is a First Response in a Cancerous Pathway: Hyperplasia Developments to 4n Cell Cycling in Dysplasia Linked to Rb-Inactivation 被引量:5
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作者 Kirsten H. Walen 《Journal of Cancer Therapy》 2015年第10期906-916,共11页
In a series of publications, the hypothesis of a special-type of endo-polyploidy, marked by 4-chromatid chromosomes (diplochromosomes), in the initiation of tumorigenesis has been presented from in vitro experiments. ... In a series of publications, the hypothesis of a special-type of endo-polyploidy, marked by 4-chromatid chromosomes (diplochromosomes), in the initiation of tumorigenesis has been presented from in vitro experiments. This review uses cellular happenings in benign pre-neoplasia to substantiate this idea, which appears to be linked to the wound-healing process of injured tissue. Rarer association between a wound healing process and a cancer occurrence has long been known. The wound healing multi-program-system involved a phase of tetraploidy that showed diplochromosomes. The hypothesis is that the inflammatory phase may not always be sufficient in getting rid of dead and damaged cells (by apoptosis and autophagy), such that cells with genomic damage (DNA breakage) may survive by genomic repair associated with change to diplochromosomal tetraploidy. In vitro data have shown division of these cells to be an orderly, mechanistic two-step, meiotic-like system, resulting in only two types of progeny cells: 4n/4C/G1 and 2n/2C/G1 pseudo-diploid cells with hyperplastic-like growth-morphology. In vivo damage to tissues can be from many sources for example, physical, toxic environment or from a disease as in Barrett’s esophagus (BE) with acid reflux into the esophagus. For this condition, it is acknowledged that damage of the esophagus lining is a pre-condition to hyperplastic lesions of pre-neoplasia. These initial lesions were from “diploid” propagating cells and, 4n cells with G2 genomic content (no mitosis) accumulated in these lesions before a change to dysplasia. Cell cycle kinetics put these 4n cells in G1, which with S-phase entry would lead to asymmetric tetraploid mitoses, characteristic for dysplastic lesions. This change in hyperplasia to dysplasia is the root-essential condition for a potential progression of pre-neoplasia to cancer. In BE the hyperplastic lesion showed increasing gains of cells with inactivated p53 and p16[ink4a] genes, which destroyed the retinoblastoma (Rb) protein-control over S-phase entry from G1. Rb-protein is a key controller of cycling advancement from G1 (also for normal cells), and is frequently inactivated in tumor cells. Thus in BE, 4n/4C/G1 cells with mutated p53 and p16[ink4a] genes gained cycling ability to tetraploid aneuploid cell cycles, which constituted the change from hyperplasia to dysplastic lesions. In general, such lesions have high predictive value for a cancerous change. Proliferation rates of pre-neoplasia and progression have been shown to be increased by a component of the wound healing program. 展开更多
关键词 MITOTIC SLIPPAGE Endotetraploidization Diplochromosomes Meiotic-Like Division 4n/4C/G1 PROGENY PROLIFERATIVE Advantage INACTIVATED p53 p16[ink4a]
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Naturally Crosslinked Biocompatible Carbonaceous Liquid Metal Aqueous Ink Printing Wearable Electronics for Multi-Sensing and Energy Harvesting 被引量:1
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作者 King Yan Chung Bingang Xu +3 位作者 Di Tan Qingjun Yang Zihua Li Hong Fu 《Nano-Micro Letters》 SCIE EI CAS CSCD 2024年第8期148-168,共21页
Achieving flexible electronics with comfort and durability comparable to traditional textiles is one of the ultimate pursuits of smart wearables.Ink printing is desirable for e-textile development using a simple and i... Achieving flexible electronics with comfort and durability comparable to traditional textiles is one of the ultimate pursuits of smart wearables.Ink printing is desirable for e-textile development using a simple and inexpensive process.However,fabricating high-performance atop textiles with good dispersity,stability,biocompatibility,and wearability for high-resolution,large-scale manufacturing,and practical applications has remained challenging.Here,waterbased multi-walled carbon nanotubes(MWCNTs)-decorated liquid metal(LM)inks are proposed with carbonaceous gallium–indium micro-nanostructure.With the assistance of biopolymers,the sodium alginate-encapsulated LM droplets contain high carboxyl groups which non-covalently crosslink with silk sericin-mediated MWCNTs.E-textile can be prepared subsequently via printing technique and natural waterproof triboelectric coating,enabling good flexibility,hydrophilicity,breathability,wearability,biocompatibility,conductivity,stability,and excellent versatility,without any artificial chemicals.The obtained e-textile can be used in various applications with designable patterns and circuits.Multi-sensing applications of recognizing complex human motions,breathing,phonation,and pressure distribution are demonstrated with repeatable and reliable signals.Self-powered and energy-harvesting capabilities are also presented by driving electronic devices and lighting LEDs.As proof of concept,this work provides new opportunities in a scalable and sustainable way to develop novel wearable electronics and smart clothing for future commercial applications. 展开更多
关键词 BIOCOMPATIBLE Conductive ink BIOPOLYMER E-textile Carbonaceous liquid metal
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Copper Oxide Inked with Dye Obtained from the Lactarius Indigo Fungus for Energy Production
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作者 Abigail Bautista Castañeda Maria Guadalupe Montes De Oca +4 位作者 Dulce Yolotzin Medina Velazquez Jorge Iván Aldana González Pedro Adrian Martinez Montolla Joan Reyes Miranda Miguel Angel Barron 《Journal of Materials Science and Chemical Engineering》 CAS 2022年第11期36-51,共16页
The results obtained from the characterization of a copper deposit on indium doped tin oxide (ITO), inked with natural dye extracted from the Lactarius indigo fungus, for use in Gratzel type solar cells are reported. ... The results obtained from the characterization of a copper deposit on indium doped tin oxide (ITO), inked with natural dye extracted from the Lactarius indigo fungus, for use in Gratzel type solar cells are reported. An electrolyte composed of 0.1 M HNO<sub>3</sub> and 0.5 M CuSO<sub>4</sub> was used, this solution was prepared for copper deposits on the ITO. Cyclic voltammetry was performed at different scan rates to obtain the reduction zone for deposition between potentials of ?100 to ?500 mV. The dye was obtained from the indigo Lactarius fungus from maceration, once the inked deposits were obtained, characterizations were performed, the initial test was to obtain the Ultraviolet-Visible (UV-visible) of the pure dye, and later the same test was performed on the inked oxide. Electrochemical Impedance Spectroscopy (EIS) was performed on the samples, as well as Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM), to characterize the material properties for its application. 展开更多
关键词 Copper Oxide Ink Dye Energy Production Indigo Milk Mushroom
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Establishment of enzyme-linked immunosorbent assay for beef and lamb contents in cooked meat 被引量:2
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作者 Yujing Li Jingjing Liu +6 位作者 Sufang Fan Li Zhao Jing Zhang Erjing Zhang Ziran Li Yan Zhang Chunsheng Li 《Journal of Future Foods》 2024年第1期91-96,共6页
In this study,an enzyme 1linked immunosorbent assay(ELISA)was established to detect beef and 1amb components,and its performance was tested.Double-antibody sandwich ELISA was adopted and determined a coating concentra... In this study,an enzyme 1linked immunosorbent assay(ELISA)was established to detect beef and 1amb components,and its performance was tested.Double-antibody sandwich ELISA was adopted and determined a coating concentration of capture antibody 3G5 of 1:4000,a working concentration of enzyme-labeled antibody 2E7-horseradish peroxidase(HRP)of 1:1000,a sample incubation time of 60 min and a detection antibody reaction time of 60 min.The specificity,sensitivity,repeatability and stability of this assay were detemmined.The limit of detection for beef and 1amb skeleta1 muscle troponin I was 45 mg/kg,the inter-assay and intra-assay recovery rates ranged from 80.4%to 115.7%,the coefficients of variation were below 13.6%,and the cIoss reaction rates of the tissue components of chicken,duck and fish were below 13.4%.The sandwich ELISA method established in this study is stable and has high accuracy.The test results were consistent with the polymerase chain reaction(PCR)method at 50 and 100 g/kg-Therefore,this ELISA method can be used to quantitatively detect beef and 1amb components in meat products. 展开更多
关键词 Double antibody sandwich enzyme-1inked immunosorbent assay(ELISA) Beef components Lamb components
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DETECTION OF RIBGRASS MOSAIC VIRUS WITH THE TECHNIQUE OF ENZYME-LINKED CELLULOSE ACETATEMEMBRANE IMMUNOELECTROPHORESIS (ELCAIE)
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作者 朱培坤 陆妙康 《Chinese Science Bulletin》 SCIE EI CAS 1982年第6期670-672,共3页
An improved method of immuno-electrophoresis, named enzyme-linked cellulose acetatemembrane immuno-electrophoresis (ELCAIE), is reported here for the detection of ribgrass mosaic virus with high sensitivity and rapidi... An improved method of immuno-electrophoresis, named enzyme-linked cellulose acetatemembrane immuno-electrophoresis (ELCAIE), is reported here for the detection of ribgrass mosaic virus with high sensitivity and rapidity. Enzyme-linked antibody was used in combination with immuno-electrophoresis on cellulose acetatemembrane. 展开更多
关键词 cellulose MOSAIC Enzyme Freund DILUTED inked HORSE saturated TITER AGAROSE
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结肠癌合并2型糖尿病患者血清lncRNA ANRIL、miR-181b-5p的表达及其与淋巴结转移的相关性
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作者 潘勇娜 康金旺 常月锋 《实用临床医药杂志》 2025年第2期32-37,共6页
目的探讨结肠癌合并2型糖尿病(T2DM)患者血清长链非编码RNA INK4基因座中反义非编码RNA(lncRNA ANRIL)、微小RNA-181b-5p(miR-181b-5p)的表达及其与淋巴结转移的相关性。方法选取2019年5月—2022年8月本院收治的117例结肠癌合并T2DM患... 目的探讨结肠癌合并2型糖尿病(T2DM)患者血清长链非编码RNA INK4基因座中反义非编码RNA(lncRNA ANRIL)、微小RNA-181b-5p(miR-181b-5p)的表达及其与淋巴结转移的相关性。方法选取2019年5月—2022年8月本院收治的117例结肠癌合并T2DM患者为结肠癌合并T2DM组,并根据患者淋巴结转移情况将其分为淋巴结转移组56例和淋巴结未转移组61例。另选取同期体检的健康志愿者117例为对照组。采用反转录实时定量PCR检测血清lncRNA ANRIL、miR-181b-5p的相对表达水平。采用Logistic回归分析探讨影响结肠癌合并T2DM患者淋巴结转移的因素。绘制受试者工作特征(ROC)曲线分析血清lncRNA ANRIL、miR-181b-5p水平对结肠癌合并T2DM患者淋巴结转移的诊断价值。采用Pearson相关分析探讨结肠癌合并T2DM患者血清lncRNA ANRIL与miR-181b-5p表达的相关性。结果与对照组比较,结肠癌合并T2DM组患者血清lncRNA ANRIL水平升高,miR-181b-5p水平降低,差异均有统计学意义(P<0.01)。与淋巴结未转移组比较,淋巴结转移组患者血清lncRNA ANRIL水平升高,miR-181b-5p水平降低,差异均有统计学意义(P<0.01)。淋巴结转移组与淋巴结未转移组患者TNM分期、分化程度以及糖化血红蛋白(HbA1C)比较,差异有统计学意义(P<0.05)。糖尿病史、TNM分期、分化程度、HbA1C以及lncRNA ANRIL均是淋巴结转移的影响因素(P<0.05),miR-181b-5p是影响淋巴结转移的保护因素(P<0.05)。血清lncRNA ANRIL与miR-181b-5p水平呈负相关(r=-0.440,P<0.001)。ROC曲线结果显示,血清lncRNA ANRIL、miR-181b-5p以及二者联合诊断淋巴结转移的曲线下面积(AUC)分别为0.800、0.837、0.930,联合诊断价值显著高于lncRNA ANRIL(Z=2.956,P=0.003)、miR-181b-5p(Z=2.117,P=0.034)单独诊断价值。结论结肠癌合并T2DM患者血清lncRNA ANRIL水平升高,miR-181b-5p水平降低,且二者与淋巴结转移具有一定相关性。 展开更多
关键词 结肠癌 2型糖尿病 长链非编码RNA INK4基因座中反义非编码RNA 微小RNA-181b-5p
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益气解毒方下调ANRIL抑制鼻咽癌细胞增殖、迁移与侵袭
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作者 许红淼 周芳亮 +5 位作者 何兰 左涵蓉 邹芳 唐乐 江志超 何迎春 《中国中西医结合杂志》 北大核心 2025年第11期1386-1392,共7页
目的研究益气解毒方(YQJDF)是否通过下调INK4基因座中反义非编码RNA(ANRIL)干预鼻咽癌细胞的生物学效应。方法运用慢病毒转染技术构建ANRIL过表达和敲低的鼻咽癌稳定转染细胞株,再采用0.5 g/L YQJDF干预,将细胞分为过表达ANRIL(oe-ANRIL... 目的研究益气解毒方(YQJDF)是否通过下调INK4基因座中反义非编码RNA(ANRIL)干预鼻咽癌细胞的生物学效应。方法运用慢病毒转染技术构建ANRIL过表达和敲低的鼻咽癌稳定转染细胞株,再采用0.5 g/L YQJDF干预,将细胞分为过表达ANRIL(oe-ANRIL)+YQJDF组、过表达空载体(oe-NC)+YQJDF组、干扰ANRIL(sh-ANRIL)+YQJDF组、干扰空载体(sh-NC)+YQJDF组,每组设3个重复。实时细胞分析技术(RTCA)检测细胞增殖能力,划痕实验检测细胞迁移能力,Matrigel侵袭小室法检测细胞侵袭能力RT-PCR检测ANRIL的表达水平。结果RTCA检测显示,与空载体(oe-NC)组比较,oe-ANRIL组细胞的增殖速度增高(P<0.01),相对增殖率提高35%。划痕与侵袭实验进一步证实,oe-ANRIL细胞迁移和侵袭能力增强(P<0.01)。与oe-NC+YQJDF组比较,过表达ANRIL后,益气解毒方抑制细胞增殖、迁移和侵袭的效应均降低(P<0.05);沉默ANRIL后,YQJDF抑制细胞增殖、迁移效应均增强(P<0.05)。结论ANRIL促进鼻咽癌细胞的增殖、迁移与侵袭,YQJDF通过靶向抑制ANRIL的表达发挥抗鼻咽癌的作用。 展开更多
关键词 益气解毒方 鼻咽癌 INK4基因座中反义非编码RNA 侵袭 迁移 中药复方 中西医结合
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Synthesis of p-type PbS quantum dot ink via inorganic ligand exchange in solution for high-efficiency and stable solar cells 被引量:1
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作者 Napasuda Wichaiyo Yuyao Wei +9 位作者 Chao Ding Guozheng Shi Witoon Yindeesuk Liang Wang Huān Bì Jiaqi Liu Shuzi Hayase Yusheng Li Yongge Yang Qing Shen 《Journal of Semiconductors》 2025年第4期63-70,共8页
Traditional p-type colloidal quantum dot(CQD)hole transport layers(HTLs)used in CQD solar cells(CQDSCs)are commonly based on organic ligands exchange and the layer-by-layer(LbL)technique.Nonetheless,the ligand detachm... Traditional p-type colloidal quantum dot(CQD)hole transport layers(HTLs)used in CQD solar cells(CQDSCs)are commonly based on organic ligands exchange and the layer-by-layer(LbL)technique.Nonetheless,the ligand detachment and complex fabrication process introduce surface defects,compromising device stability and efficiency.In this work,we propose a solution-phase ligand exchange(SPLE)method utilizing inorganic ligands to develop stable p-type lead sulfide(PbS)CQD inks for the first time.Various amounts of tin(Ⅱ)iodide(SnI_(2))were mixed with lead halide(PbX_(2);X=I,Br)in the ligand solution.By precisely controlling the SnI_(2)concentration,we regulate the transition of PbS QDs from n-type to p-type.PbS CQDSCs were fabricated using two different HTL approaches:one with 1,2-ethanedithiol(EDT)-passivated QDs via the LbL method(control)and another with inorganic ligand-passivated QD ink(target).The target devices achieved a higher power conversion efficiency(PCE)of 10.93%,compared to 9.83%for the control devices.This improvement is attributed to reduced interfacial defects and enhanced carrier mobility.The proposed technique offers an efficient pathway for producing stable p-type PbS CQD inks using inorganic ligands,paving the way for high-performance and flexible CQD-based optoelectronic devices. 展开更多
关键词 quantum dot solar cells hole transport layer PBS p-type ink inorganic ligands
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4D-printed snake-like biomimetic soft robots 被引量:1
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作者 Xingcheng Ou Jiaqi Huang +6 位作者 Dantong Huang Xiaohong Li Guoliang Chen Yabin Yang Ran Bi Yu Sheng Shuang-Zhuang Guo 《Bio-Design and Manufacturing》 2025年第1期55-67,I0018-I0038,共34页
Wireless millirobots engineered to infiltrate intricate vascular networks within living organisms,particularly within constricted and confined spaces,hold immense promise for the future of medical treatments.However,w... Wireless millirobots engineered to infiltrate intricate vascular networks within living organisms,particularly within constricted and confined spaces,hold immense promise for the future of medical treatments.However,with their multifaceted and intricate designs,some robots often grapple with motion and functionality issues when confronted with tight spaces characterized by small cross-sectional dimensions.In this study,drawing inspiration from the high aspect ratio and undulating swimming patterns of snakes,a millimeter-scale,snake-like robot was designed and fabricated via a combination of extrusion-based four-dimensional(4D)printing and magnetic-responsive intelligent functional inks.A sophisticated motion control strategy was also developed,which enables the robots to perform various dynamic movements,such as undulating swimming,precise turns,graceful circular motions,and coordinated cluster movements,under diverse magnetic field variations.As a potential application,the snake robot can navigate and release drugs in a model coronary intervention vessel with tortuous channels and fluid filling.The novel design and promising applications of this snake robot are invaluable tools in future medical surgeries and interventions. 展开更多
关键词 4D printing Magnetic-responsive ink Untethered medical soft robot Snake-like robot Drug delivery
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胆囊癌组织p16INK4a基因甲基化状态和蛋白表达及其与预后的相关性 被引量:1
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作者 石明亮 王晓磊 +2 位作者 郭伟 朱硕 申洋 《社区医学杂志》 2025年第9期292-298,共7页
目的探讨p16INK4a基因甲基化状态及蛋白表达与胆囊癌临床特征及其与预后的相关性。方法收集2018-01-01-2019-12-30河南大学第一附属医院收治的115例胆囊癌患者的临床资料,采用直接测序法检测癌组织基因组DNA p16INK4a基因甲基化水平,免... 目的探讨p16INK4a基因甲基化状态及蛋白表达与胆囊癌临床特征及其与预后的相关性。方法收集2018-01-01-2019-12-30河南大学第一附属医院收治的115例胆囊癌患者的临床资料,采用直接测序法检测癌组织基因组DNA p16INK4a基因甲基化水平,免疫组化法检测组织p16INK4a蛋白表达;分析不同临床病理特征癌组织p16INK4a基因甲基化状态及蛋白表达差异;Kaplan-Meier法分析不同p16INK4a基因甲基化状态及蛋白表达者生存差异;Cox比例风险模型分析胆囊癌患者预后影响因素。结果胆囊癌组织p16INK4a基因甲基化阳性率为56.52%(65/115),高于癌旁组织的18.26%(21/115),χ^(2)=35.956,P<0.001。胆囊癌组织p16INK4a蛋白表达阳性率为26.09%(30/115),低于癌旁组织61.74%(71/115),χ^(2)=24.305,P<0.001。低中分化、Ⅲ期患者的癌组织p16INK4a基因甲基化阳性率高于高分化、Ⅰ~Ⅱ期,p16INK4a蛋白阳性表达率低于高分化、Ⅰ~Ⅱ期,均P<0.05。随访期间73例患者死亡,p16INK4a蛋白阳性表达(63.33%)、p16INK4a基因甲基化阴性(54.00%)胆囊癌患者3年总生存率高于p16INK4a蛋白阴性表达(27.06%)、p16INK4a基因甲基化阳性23.07%。TNM分期(HR=3.572,95%CI:1.995~6.393)、淋巴结转移(HR=2.396,95%CI:1.317~4.356)、组织分化程度(HR=2.628,95%CI:1.606~4.298)、p16INK4a基因甲基化(HR=1.915,95%CI:1.362~2.693)、p16INK4a蛋白(HR=0.773,95%CI:0.659~0.906)是胆囊癌死亡的影响因素,均P<0.05。结论p16INK4a基因启动子甲基化状态及蛋白表达与胆囊癌TNM分期、组织分化程度及预后有关,可能参与胆囊癌发生发展。 展开更多
关键词 胆囊癌 P16INK4A DNA甲基化 免疫组织化学 预后因素
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T_(2)mapping对止痛健骨方防治兔膝骨关节炎关节软骨衰老疗效的监测
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作者 高辉 黎芳丽 +1 位作者 颜路悠 张堃 《中国中西医结合影像学杂志》 2025年第4期456-460,共5页
目的:评估止痛健骨方防治膝骨关节炎关节软骨衰老的疗效,探讨T_(2)mapping对疗效监测的价值。方法:采用石膏绷带伸直位制动膝关节的方法制作新西兰大白兔膝骨关节炎模型,将32只新西兰大白兔随机分为正常对照组、模型对照组、止痛健骨方... 目的:评估止痛健骨方防治膝骨关节炎关节软骨衰老的疗效,探讨T_(2)mapping对疗效监测的价值。方法:采用石膏绷带伸直位制动膝关节的方法制作新西兰大白兔膝骨关节炎模型,将32只新西兰大白兔随机分为正常对照组、模型对照组、止痛健骨方组及硫酸氨基葡萄糖组各8只。制动2周后,正常对照组和模型对照组给予相同容积的蒸馏水灌胃,止痛健骨方组给予止痛健骨方,硫酸氨基葡萄糖组给予硫酸及葡萄糖胶囊。治疗4周后均行膝关节T_(2)mapping成像,并对关节软骨细胞的p16^(INK4a)表达量进行免疫组化分析。比较各组间膝关节软骨T_(2)值及p16^(INK4a)表达量的差异,并分析不同干预方法、关节软骨T_(2)值与关节软骨细胞p16^(INK4a)表达量的相关性。结果:止痛健骨方组、硫酸氨基葡萄糖组关节软骨T_(2)值均高于正常对照组,低于模型对照组(均P<0.05);正常对照组、止痛健骨方组、硫酸氨基葡萄糖组关节软骨细胞p16^(INK4a)表达量均明显低于模型对照组(均P<0.05),但3组间差异无统计学意义(P>0.05)。软骨细胞p16^(INK4a)表达量与关节软骨T_(2)值呈非线性关系,p16^(INK4a)表达量随T_(2)值升高,先缓慢升高,再迅速升高(P<0.05)。结论:止痛健骨方可防治膝骨关节炎关节软骨衰老,且T_(2)mapping可作为药物防治软骨衰老的无创评估工具。 展开更多
关键词 骨性关节炎 关节软骨 止痛健骨方 磁共振成像 p16^(INK4a)
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INK4基因座中反义非编码RNA对乳腺癌细胞增殖能力及顺铂敏感性的影响
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作者 韩一菲 张芳芳 +4 位作者 王靖楠 邹炎 应莉 谢依天 李娜 《新乡医学院学报》 2025年第2期93-98,106,共7页
目的探讨INK4基因座中反义非编码RNA(ANRIL)对乳腺癌细胞增殖能力及顺铂敏感性的影响及其作用机制。方法采用实时荧光定量聚合酶链反应(RT-qPCR)技术检测乳腺癌组织标本及配对的癌旁组织、人乳腺癌细胞株MCF-7、MDA-MB-231、SKBR3以及... 目的探讨INK4基因座中反义非编码RNA(ANRIL)对乳腺癌细胞增殖能力及顺铂敏感性的影响及其作用机制。方法采用实时荧光定量聚合酶链反应(RT-qPCR)技术检测乳腺癌组织标本及配对的癌旁组织、人乳腺癌细胞株MCF-7、MDA-MB-231、SKBR3以及人正常乳腺上皮细胞株MCF-10A中ANRIL的表达;将对数生长期的MDA-MB-231细胞随机分为si-NC组(转染对照的空载siRNA)、si-ANRIL组(转染ANRIL干扰片段)、si-ANRIL-anti-NC组(共转染ANRIL的干扰片段和miR-7-5p的空载体)、si-ANRIL-anti-miR-7-5p组(转染ANRIL的干扰片段和miR-7-5p的干扰片段)、miR-NC组(转染对照miRNA的空载体)、miR-7-5p mimic组(转染miR-7-5p的过表达载体)、miR-7-5p+ANRIL-WT转染组(共转染miR-7-5p mimics和ANRIL-WT)、miR-NC+ANRIL-WT转染组(共转染mimics Control和ANRIL-WT)、miR-7-5p+ANRIL-MUT转染组(共转染miR-7-5p mimics和ANRIL-MUT)和miR-NC+ANRIL-WUT转染组(共转染mimics Control和ANRIL-MUT)。采用细胞计数试剂盒-8法检测乳腺癌细胞的体外增殖能力,克隆形成实验检测细胞对顺铂的敏感性,双荧光素酶报告实验和RNA免疫沉淀(RIP)实验检测ANRIL和miR-7-5p的靶向结合关系。结果乳腺癌组织中ANRIL mRNA相对表达量显著高于癌旁组织(P<0.05);MDA-MB-231、MCF-7和SKBR3细胞中ANRIL mRNA的相对表达量显著高于正常乳腺上皮细胞(P<0.05);MDA-MB-231细胞中ANRIL mRNA的表达量显著高于MCF-7和SKBR3细胞(P<0.05)。培养24、48、72 h时,si-ANRIL组细胞存活率显著低于si-NC组(P<0.05)。与si-NC组相比,si-ANRIL组细胞克隆形成数目显著降低(P<0.05)。与miR-NC+ANRIL-WT转染组相比,miR-7-5p+ANRIL-WT转染组细胞荧光素酶活性显著降低(P<0.05);miR-NC+ANRIL-MUT转染组与miR-7-5p+ANRIL-MUT转染组细胞荧光素酶活性比较差异无统计学意义(P>0.05)。miR-7-5p mimic组细胞中ANRIL富集量显著高于miR-NC组(P<0.05)。在培养24、48、72 h时,si-ANRIL-anti-miR-7-5p组MDA-MB-231细胞存活率显著高于si-ANRIL-anti-NC组(P<0.05)。si-ANRIL-anti-miR-7-5p组MDA-MB-231细胞克隆形成数目显著高于si-ANRIL-anti-NC组(P<0.05)。与si-ANRIL-anti-NC组相比,si-ANRIL-anti-miR-7-5p组的MDA-MB-231细胞对顺铂的敏感性降低了4.04倍。结论ANRIL在乳腺癌组织和乳腺癌细胞中呈高表达,并通过下调miR-7-5p的表达来促进乳腺癌细胞的增殖,降低乳腺癌细胞对顺铂的敏感性。 展开更多
关键词 INK4基因座中反义非编码RNA 微RNA-7-5p 乳腺癌 生物学行为 顺铂敏感性
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Constructing Ti_(3)C_(2)T_(x)-MXene-based gradient woodpile structure by direct ink writing 3D printing for efficient microwave absorption 被引量:1
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作者 Changtian Zhu Pei Liu +8 位作者 Jin Chen Zixuan Ding Guohui Tang Qingqing Gao Yinxu Ni Kai Xu Zhilei Hao Gaojie Xu Fenghua Liu 《International Journal of Minerals,Metallurgy and Materials》 2025年第3期657-667,共11页
As a novel 2D material,Ti_(3)C_(2)T_(x)-MXene has become a major area of interest in the field of microwave absorption(MA).However,the MA effect of common Ti_(3)C_(2)T_(x)-MXene is not prominent and often requires com... As a novel 2D material,Ti_(3)C_(2)T_(x)-MXene has become a major area of interest in the field of microwave absorption(MA).However,the MA effect of common Ti_(3)C_(2)T_(x)-MXene is not prominent and often requires complex processes or combinations of other ma-terials to achieve enhanced performance.In this context,a kind of gradient woodpile structure using common Ti_(3)C_(2)T_(x)-MXene as MA ma-terial was designed and manufactured through direct ink writing(DIW)3D printing.The minimum reflection loss(RL_(min))of the Ti_(3)C_(2)T_(x)-MXene-based gradient woodpile structures with a thickness of less than 3 mm can reach-70 dB,showing considerable improve-ment compared with that of a completely filled structure.In addition,the effective absorption bandwidth(EAB)reaches 7.73 GHz.This study demonstrates that a Ti_(3)C_(2)T_(x)-MXene material with excellent MA performance and tunable frequency band can be successfully fab-ricated with a macroscopic structural design and through DIW 3D printing without complex material hybridization and modification,of-fering broad application prospects by reducing electromagnetic wave radiation and interference. 展开更多
关键词 Ti_(3)C_(2)T_(x)-MXene electromagnetic wave absorption macro-design impedance matching 3D printing ink
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Research on Printing Preferences for Skin Color
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作者 WANG Dan GONG Xu-ping +1 位作者 SHANG Xiao-yu HUANG Min 《印刷与数字媒体技术研究》 北大核心 2025年第1期46-52,共7页
In order to study the preferred skin color for printing images,two CMYK images from ISO 400 and one from iStock,including five skin color images of East Asian females was selected in this study.The images were adjuste... In order to study the preferred skin color for printing images,two CMYK images from ISO 400 and one from iStock,including five skin color images of East Asian females was selected in this study.The images were adjusted with the CMYK printing ink volume variation of the single,double and triple channels in the given 280%total ink limit conditions.A larger number of color vision normal observers were organized to carry out the color preference evaluation experiment,and the selected preferred skin colors were analyzed.The distribution range of the chromaticity values for skin color images were obtained and the results indicated that there are three regions for printing skin color preferences,and the observers have a memory preference for brighter,fairer skin colors in young female and a reddish skin colors in girl,which can provide the guidance for color adjustment of printed skin color images. 展开更多
关键词 Preferred skin color Printing images Total ink limit CMYK ink adjustment
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An Initial Exploration of Digital Ink Texture Construction Methods Based on Blender Material Nodes
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作者 XIN Bei XU Zhendong 《Cultural and Religious Studies》 2025年第1期26-32,共7页
This study examines the development of painting techniques of Chinese ink wash landscape paintings,pays attention to its unique brush and ink language and features of the representation of elements,and deeply analyzes... This study examines the development of painting techniques of Chinese ink wash landscape paintings,pays attention to its unique brush and ink language and features of the representation of elements,and deeply analyzes the artistic characteristics of digital ink wash texture materials.The research focuses on key aspects such as the ink brushstrokes with the combination of emptiness and reality,the profound and serene ink wash space,and the extremely ingenious position layout.It proposes a construction path of digital ink wash texture materials based on the Blender material node system.This method makes use of the flexibility of the Blender material node system to successfully simulate highly realistic digital ink wash textures.It can not only construct static ink wash textures but also realize the dynamic transformation of static ink wash works through animation nodes and procedural control,thereby enhancing the artistic expression of digital ink wash works.The proposal and implementation of this method expand the application scope of the Blender material node system,help deeply explore the potential of digital ink wash art,and open up a brand new research path for constructing digital ink wash textures. 展开更多
关键词 Blender material node digital ink ink textures
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Developments and Challenges in Direct Ink Writing for High-Performance Polymers
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作者 Zhangzhang Tang Yaoming Zhang +4 位作者 Liming Tao Zenghui Yang Peng Liu Qihua Wang Tingmei Wang 《Additive Manufacturing Frontiers》 2025年第2期111-133,共23页
Compared to subtractive manufacturing and casting,3D printing(additive manufacturing)offers advantages,such as the rapid production of complex structures,reduced material waste,and environmental friendliness.Direct in... Compared to subtractive manufacturing and casting,3D printing(additive manufacturing)offers advantages,such as the rapid production of complex structures,reduced material waste,and environmental friendliness.Direct ink writing(DIW)is one of the most popular 3D printing techniques owing to its ability to print multiple materials simultaneously and its high compatibility with printing inks.However,DIW presents significant challenges,particularly in the printing of high-performance polymers.The main challenges are as follows:1.The rigid structures and reaction kinetics of high-performance polymers make developing new inks difficult.2.The limited types of available high-performance polymers underscore the need for new DIW-suitable materials.3.Layer-by-layer stacking weakens interlayer bonding,affecting the mechanical properties of the printed product.4.The accuracy and speed of DIW printing are insufficient for large-scale manufacturing.After introducing the topic,the requirements for DIW printing inks are first reviewed,emphasizing the importance of thixotropic agents.Then,research progress regarding DIW printing of high-performance polymers is comprehensively reviewed according to the requirements of different polymer inks.Additionally,the applications of these materials across various fields are summarized.Finally,the challenges in DIW printing of high-performance polymers,along with corresponding solutions and future development prospects,are discussed in detail. 展开更多
关键词 3D printing Direct ink writing Printing ink Polymer material High-performance polymer
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WHICH IS BETTER
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《空中英语教室(初级版.大家说英语)》 2025年第9期20-20,56,共2页
Conversation B Susie:I know.Pencils are also better for drawing.You can make lines lighter or darker.Sam:But you have to sharpen pencils or add lead to them.Susie:You do.But pencils last longer than pens.Pen ink can d... Conversation B Susie:I know.Pencils are also better for drawing.You can make lines lighter or darker.Sam:But you have to sharpen pencils or add lead to them.Susie:You do.But pencils last longer than pens.Pen ink can dry out,but pencil lead doesn’t.Sam:But words look neater when you write in pen.Susie:They do.So,which pen do you want?Sam:One with blue ink,thanks. 展开更多
关键词 DRAWING PENCILS INK lead PENS WRITING
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Direct Ink Writing Method of Fractal Wearable Flexible Sensor Based on Conductive Graphene/Polydimethylsiloxane Ink
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作者 CHEN Junling GAO Feiyang +1 位作者 ZHANG Liming ZHENG Xiongfei 《Journal of Shanghai Jiaotong university(Science)》 2025年第1期18-26,共9页
Flexible electronic technology has laid the foundation for complex human-computer interaction system,and has attracted great attention in the field of human motion detection and soft robotics.Graphene has received an ... Flexible electronic technology has laid the foundation for complex human-computer interaction system,and has attracted great attention in the field of human motion detection and soft robotics.Graphene has received an extensive attention due to its excellent electrical conductivity;however,how to use it to fabricate wearable flexible sensors with complex structures remains challenging.In this study,we studied the rheological behavior of graphene/polydimethylsiloxane ink and proposed an optimal graphene ratio,which makes the ink have an good printability and conductivity at the same time.Then,based on the theory of Peano fractal layout,we proposed a two-dimensional structure that can withstand multi-directional tension by replacing the traditional arris structure with the arc structure.After that,we manufactured circular arc fractal structure sensor by adjusting ink composition and printing structure through direct ink writing method.Finally,we evaluated the detection performance and repeatability of the sensor.This method provides a simple and effective solution for fabricating wearable flexible sensors and exhibits the potential to fabricate 3D complex flexible electronic devices. 展开更多
关键词 direct ink writing conductive graphene ink wearable flexible sensor strain sensing fractal structure
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Sixty Years of Cultural Flourishing in Bookstores
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作者 Zhao Yue(Text/Photos) Qin Pan(Text/Photos) 《China's Tibet》 2025年第4期16-21,共6页
As sunlight streams through floor-to-ceiling windows and falls gently upon the bookshelves,readers-seated or standing-immerse themselves in the rich literary world created by ink and paper.
关键词 ink paper BOOKSTORE cultural prosperity bookshelves READERS SUNLIGHT
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High Performance Gas-sensitive Ink for Non-contact Dispenser Printing MEMS Gas Sensor
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作者 WANG Jianbo ZHANG Ning +3 位作者 JIA Minghao QI Yanyuan YANG Shuang JIN Wei 《Journal of Wuhan University of Technology(Materials Science)》 2025年第4期931-938,共8页
This study systemmatically investigated the effects of solid content and dispersant content on the physicochemical properties of ZnO-SnO_(2) composite ink.The experimental results show that even with the use of low-mo... This study systemmatically investigated the effects of solid content and dispersant content on the physicochemical properties of ZnO-SnO_(2) composite ink.The experimental results show that even with the use of low-molecular-weight PEG400 dispersant,gas-sensitive ink with high solid content and good suspension stability can be obtained,which is advantageous for low-temperature film formation and can effectively prevent property changes and film crack of high-temperature-sintering-induced material.Under this condition,the ink at a 15wt%solid content and 2wt%-10wt%PEG400 has good film-forming ability and high adhesion strength on the micro-electromechanical system(MEMS)micro-hotplates.Especially,the MEMS sensor printed using the ink of 6wt%PEG400 shows highest sensitivity,favorable impact resistance,thermal shock resistance,and up to 8 years of service life. 展开更多
关键词 MEMS INK physicochemical parameters PEG400 gas sensor
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