After many years of development, die casting technology of metallic materials has been matured. In this paper,the lower-support and its injecting system were created with commercial software. And then the simulation s...After many years of development, die casting technology of metallic materials has been matured. In this paper,the lower-support and its injecting system were created with commercial software. And then the simulation softwareFLOW3D was applied to study the flow behavior of the melt during injection filling process. Both temperature field anddefect distribution were simulated. Based on these results, the better injecting system with two additional overflows wasadopted.展开更多
In the scenario of a steam generator tube rupture accident in a lead-cooled fast reactor,secondary circuit subcooled water under high pressure is injected into an ordinary-pressure primary vessel,where a molten lead-b...In the scenario of a steam generator tube rupture accident in a lead-cooled fast reactor,secondary circuit subcooled water under high pressure is injected into an ordinary-pressure primary vessel,where a molten lead-based alloy(typically pure lead or lead-bismuth eutectic(LBE))is used as the coolant.To clarify the pressure build-up characteristics under water-jet injection,this study conducted several experiments by injecting pressurized water into a molten LBE pool at Sun Yat-sen University.To obtain a further understanding,several new experimental parameters were adopted,including the melt temperature,water subcooling,injection pressure,injection duration,and nozzle diameter.Through detailed analyses,it was found that the pressure and temperature during the water-melt interaction exhibited a consistent variation trend with our previous water-droplet injection mode LBE experiment.Similarly,the existence of a steam explosion was confirmed,which typically results in a much stronger pressure build-up.For the non-explosion cases,increasing the injection pressure,melt-pool temperature,nozzle diameter,and water subcooling promoted pressure build-up in the melt pool.However,a limited enhancement effect was observed when increasing the injection duration,which may be owing to the continually rising pressure in the interaction vessel or the isolation effect of the generated steam cavity.Regardless of whether a steam explosion occurred,the calculated mechanical and kinetic energy conversion efficiencies of the melt were relatively small(not exceeding 4.1%and 0.7%,respectively).Moreover,the range of the conversion efficiency was similar to that of previous water-droplet experiments,although the upper limit of the jet mode was slightly lower.展开更多
Sepsis-associated acute kidney injury(SA-AKI)represents a leading contributor to the elevated mortality observed in sepsis patients.In the present study,we conducted a comprehensive meta-analysis to assess the clinica...Sepsis-associated acute kidney injury(SA-AKI)represents a leading contributor to the elevated mortality observed in sepsis patients.In the present study,we conducted a comprehensive meta-analysis to assess the clinical effectiveness of continuous renal replacement therapy(CRRT)in combination with Xuebijing injection for the treatment of SA-AKI.A systematic search was performed across major databases,including CNKI,VIP,Sinomed,WanFang,PubMed,and the Cochrane Library,covering publications up to January 2025.Quality assessment and statistical analyses were carried out using RevMan 5.4.The pooled results demonstrated that the adjunctive use of Xuebijing injection with CRRT,as compared to CRRT alone,yielded significantly better outcomes.Specifically,this combined therapy was associated with enhanced recovery of renal function,more effective clearance of inflammatory mediators,improved immune modulation,better regulation of coagulation parameters,and a marked reduction in mortality among SA-AKI patients.展开更多
Dear Editor,The attacker is always going to intrude covertly networked control systems(NCSs)by dynamically changing false data injection attacks(FDIAs)strategy,while the defender try their best to resist attacks by de...Dear Editor,The attacker is always going to intrude covertly networked control systems(NCSs)by dynamically changing false data injection attacks(FDIAs)strategy,while the defender try their best to resist attacks by designing defense strategy on the basis of identifying attack strategy,maintaining stable operation of NCSs.To solve this attack-defense game problem,this letter investigates optimal secure control of NCSs under FDIAs.First,for the alterations of energy caused by false data,a novel attack-defense game model is constructed,which considers the changes of energy caused by the actions of the defender and attacker in the forward and feedback channels.展开更多
[Objectives]To investigate the clinical efficacy of acupoint injection of nerve growth factors combined with task-oriented training for patients with post-stroke hemiplegic gait,and quantitatively evaluate the outcome...[Objectives]To investigate the clinical efficacy of acupoint injection of nerve growth factors combined with task-oriented training for patients with post-stroke hemiplegic gait,and quantitatively evaluate the outcomes using the Gait Watch analysis system.[Methods]A total of 90 patients with post-stroke hemiplegia,who were hospitalized at the Rehabilitation Center of Taihe Hospital between January 2023 and December 2023,were selected for this study.The participants were randomly assigned to three groups:the task-oriented rehabilitation training group(control group I,n=30),the ordinary acupuncture combined with task-oriented training group(control group II,n=30),and the acupoint injection combined with task-oriented training group(observation group,n=30).Each group underwent treatment for 4 weeks.The Gait Watch analysis system was employed to assess the spatiotemporal gait parameters of the patients prior to treatment,as well as 2 weeks post treatment and 4 weeks post treatment.The efficacy of the treatment was subsequently analyzed.[Results]After 4 weeks of treatment,the spatiotemporal gait parameters,specifically step length,step speed,step frequency,percentage of the standing phase,and percentage of the swinging phase,exhibited significant improvement in the observation group compared to those before treatment(P<0.05).Furthermore,the degree of improvement in the observation group was superior to that observed in both control group I and control group II,with the differences reaching statistical significance(P<0.05).[Conclusions]Acupoint injection combined with task-oriented training has been shown to significantly enhance gait function in patients with post-stroke hemiplegia.The Gait Watch analysis system offers an accurate and objective quantitative assessment,making it a valuable tool for clinical application and promotion.展开更多
The occurrence time and magnitude of injection-induced seismicity are influenced by engineering factors,such as wellhead pressure,injection location,injection volume,and injection rate.Understanding the relationship b...The occurrence time and magnitude of injection-induced seismicity are influenced by engineering factors,such as wellhead pressure,injection location,injection volume,and injection rate.Understanding the relationship between injection operations and seismic magnitude is of great significance for optimizing industrial production and reducing earthquake disasters.Numerical simulation of hydromechanical coupling is a crucial method for studying injection-induced seismicity.However,few studies have explored the risk management measures for injection-induced seismicity from the perspective of engineering.How seismic magnitudes can be reduced through reasonable adjustments to injection operations in engineering remains unclear.Therefore,in this study,a 3D hydro-mechanical coupling model involving multiple faults and injection wells was established based on the geological background and well location of Fox Creek,Canada.Different injection schemes under multi-well and multi-fault conditions were studied,and a traffic light system was used to simulate and control the magnitudes under a multi-well injection scheme.Specifically,we simulated injection scenarios involving up to three wells and analyzed the response of five faults.We compared the maximum moment magnitude of different scenarios by controlling the same injection volume.The results revealed the effect and advantage of the multi-well scheme in reducing seismic magnitude.To reduce the risk of induced seismicity,utilizing far-fault operational wells to compensate for the effects of near-fault operational wells proves to be an efficient and cost-effective method,with potential for wide practical applications.展开更多
BACKGROUND Sub-acromial injections are a therapeutic option for rotator cuff injuries;however,evidence regarding the most effective drug in this context is unclear,which needs to be investigated.AIM To evaluate the ef...BACKGROUND Sub-acromial injections are a therapeutic option for rotator cuff injuries;however,evidence regarding the most effective drug in this context is unclear,which needs to be investigated.AIM To evaluate the effectiveness of various sub-acromial injections for rotator cuff injuries.METHODS We conducted a systematic review and pair-wise and network meta-analyses of randomized clinical trials(RCTs)comparing sub-acromial injections for rotator cuff injuries.The interventions evaluated were hyaluronic acid(HA),platelet-rich plasma(PRP),prolotherapy,and corticosteroids.The outcomes of interest were pain and functional improvement,which were evaluated with standardized scores.The Risk of Bias 2 tool and the Grading of Recommendations,Assessment,Development and Evaluation methodology were used to assess data quality.RESULTS Twenty RCTs,comprising 1479 participants,were included.In the short term,HA achieved the best outcomes[pain mean difference(MD)=-1.48,95%confidence interval(CI)-2.37 to-0.59;function MD=10.18,95%CI:4.96-15.41].In the medium term,HA,PRP,HA+PRP,and corticosteroids were not superior to placebo in improving pain.Based on function,HA+PRP was superior to placebo,corticosteroids,and PRP(MD=26.72;95%CI:8.02-45.41).In the long term,HA,PRP,and corticosteroids were not superior to placebo in reducing pain.However,based on function,HA+PRP,PRP,and HA were superior to placebo,and HA+PRP had the best result(MD=36.64;95%CI:31.66-33.62).CONCLUSION HA provides satisfactory short-term results,while HA with PRP demonstrates functional improvement in the medium and long terms.However,no intervention maintained the pain-relief effect on>3-month follow-up.展开更多
This study elucidates the findings of a computational investigation into the stimulation characteristics of natural reservoir systems enhanced by high-voltage electropulse-assisted fluid injection.The presented method...This study elucidates the findings of a computational investigation into the stimulation characteristics of natural reservoir systems enhanced by high-voltage electropulse-assisted fluid injection.The presented methodology delineates the comprehensive rock-fracturing process induced by electropulse and subsequent fluid injection,encompassing the discharge circuit,plasma channel formation,shockwave propagation,and hydro-mechanical response.A hydromechanical model incorporating an anisotropic plastic damage constitutive law,discrete fracture networks,and heterogeneous distribution is developed to represent the natural reservoir system.The results demonstrate that high-voltage electropulse effectively generates intricate fracture networks,significantly enhances the hydraulic properties of reservoir systems,and mitigates the adverse impact of ground stress on fracturing.The stimulationenhancing effect of electropulse is observed to intensify with increasing discharge voltage,with enhancements of 118.0%,139.5%,and 169.0%corresponding to discharge voltages of 20 kV,40 kV,and 60 kV,respectively.Additionally,a high-voltage electropulse with an initial voltage of U_(0)=80 kV and capacitance C=5μF has been shown to augment the efficiency of injection activation to approximately 201.1%compared to scenarios without electropulse.Under the influence of high-voltage electropulse,the fluid pressure distribution diverges from the conventional single direction of maximum stress,extending over larger areas.These innovative methods and findings hold potential implications for optimizing reservoir stimulation in geo-energy engineering.展开更多
Background Sudden sensorineural hearing loss(SSNHL)is a rapid condition of hearing impairment,typically occurring over 72 hours and affecting the inner ear or auditory nerve pathways.The etiology of SSNHL remains larg...Background Sudden sensorineural hearing loss(SSNHL)is a rapid condition of hearing impairment,typically occurring over 72 hours and affecting the inner ear or auditory nerve pathways.The etiology of SSNHL remains largely idiopathic,with potential causes including viral infections,vascular occlusions,and autoimmune disorders.Traditional treatment primarily involves systemic corticosteroids,but their efficacy is inconsistent,leading to exploring alternative and adjunctive therapies such as intratympanic steroid(ITS)injections.Objective This Systematic Review and Meta-Analysis intends to determine the effectiveness of ITS injections as an initial treatment,combined therapy,or salvage treatment for SSNHL compared to systemic steroids alone.Methods An inclusive study was performed through the PubMed,Scopus,Web of Science,and Cochrane Library databases,encompassing publications published from 2015 to 2024.Case-control studies,cohort studies,randomized controlled trials(RCTs),and systematic reviews were included.Two separate reviewers read the studies and extracted data on steroid administration methods,study outcomes,and risk of bias(ROB)using the Cochrane Collaboration Tool and RevMan 5.4.Results The review included 12 studies with varied designs,including prospective RCTs,systematic reviews,and retrospective analyses.Combined ITS and systemic steroid therapy showed superior hearing recovery compared to systemic steroids alone in several studies.ITS alone demonstrated variable efficacy,with some studies indicating benefits,particularly as a salvage therapy.ROB assessment revealed variability in methodological rigor,with studies like those of Li&Ding et al.(2020)and Devantier et al.(2022)showing low risk,while others exhibited higher risks,particularly in random sequence generation and allocation concealment.Conclusion The findings suggest that ITS injections,particularly when combined with systemic steroids,can enhance hearing recovery in SSNHL patients.However,variability in study outcomes and methodological quality underscores the need for standardized protocols and further high-quality RCTs.This analysis underscores the prospective advantages of ITS therapy while stressing the need for stringent study designs to enhance SSNHL treatment methodologies.展开更多
This study investigates the formation feasibility of the integrated bottom car body components with dual die casting injection molding technology.During the production of a die-cast super-large one-piece body part wei...This study investigates the formation feasibility of the integrated bottom car body components with dual die casting injection molding technology.During the production of a die-cast super-large one-piece body part weighing over 10,000 t,a thorough comparison and investigation were conducted on the arising issues,using both single and double injection systems.Particular attention was given to meticulously discussing the die casting filling problems and microstructural defects that originated from the filling process.The research findings indicate that the implementation of a double injection system can significantly minimize cold shuts and reduce the solidification time.The effectiveness of this die casting technique was further confirmed by the production of high-quality castings using a scaled model that replicated real casting conditions at a 1:3 ratio,thereby maintaining a one-to-one correspondence in essential aspects.This successful study offers both theoretical insights and practical applications for the production of integrated bottom car bodies utilizing die casting in conjunction with a dual injection system.展开更多
BACKGROUND Managing sudden deafness(SD)in patients with diabetes mellitus(DM)is partic-ularly challenging due to the heightened risk of adverse effects associated with systemic drug administration.This study explores ...BACKGROUND Managing sudden deafness(SD)in patients with diabetes mellitus(DM)is partic-ularly challenging due to the heightened risk of adverse effects associated with systemic drug administration.This study explores the potential of retroauricular subperiosteal injection as a localized drug delivery method for a more effective and safe treatment.AIM To compare the efficacy of retroauricular subperiosteal injection vs systemic intravenous glucocorticoid(GC)administration for SD in patients with DM and assess the effects on blood glucose levels.METHODS A total of 128 cases of type 2 DM(T2DM)with SD diagnosed and treated in Zibo Central Hospital from February 2021 to July 2023 were divided into two groups:An observation group(66 cases receiving retroauricular subperiosteal injection of methylprednisolone)and a control group(62 cases receiving systemic intravenous administration of methylprednisolone).The two groups were compared in terms of therapeutic efficacy,hearing recovery,blood glucose level changes,and incidence of adverse reactions.Binary logistic regression was used to analyze the factors affecting therapeutic efficacy.RESULTS The observation group showed a significantly higher total effective rate(90.91%)compared with the control group(75.81%,P<0.05).Additionally,pure-tone hearing threshold,fasting plasma glucose,and 2-hour postprandial blood glucose were significantly lower in the observation group compared with the control group(P<0.05).The incidence of adverse reactions was also lower in the observation group than in the control group(7.58%vs 22.58%,P<0.05).A T2DM course longer than 5 years and systemic intravenous GC administration were identified as independent risk factors for treatment inefficacy(P<0.05).INTRODUCTION Sudden deafness(SD)is a clinical emergency characterized by rapid-onset hearing loss that is often accompanied by clinical symptoms such as tinnitus and vertigo[1].Although its pathogenesis remains unclear,it is supposedly associated with factors,including inner ear microcirculation disorders,autoimmune diseases,and viral infections[2,3].Patients with diabetes are particularly susceptible to microvascular complications due to poor long-term glycemic control,affecting the ear’s microcirculation,subsequently increasing the risk of SD[4].Type 2 diabetes mellitus(T2DM),a chronic metabolic disease,causes multiple microvascular damages throughout the body,complicating SD treatment in patients with diabetes[5,6].Inner ear microcirculation disturbances in patients with diabetes may exacerbate the risk of SD,and its pathological process may be related to vascular endothelial dysfunction,inflammatory reactions,and hemorrhological changes caused by diabetes mellitus(DM)[7].Current SD treatments include glucocorticoids(GCs),vasodilators,and hyperbaric oxygen therapy[8].GCs are widely used due to their anti-inflammatory and immunosuppressive effects[9].However,systemic GCs may cause blood glucose(BG)fluctuations and even increase the risk of complications in patients with DM,limiting their clinical use in this population[10].Therefore,there is a compelling and immediate need for a local alternative solution that minimally affects the metabolic mechanisms.In recent years,retroauricular subperiosteal injection has emerged as a localized administration modality for treating SD[11].This method allows drugs to directly act on the inner ear,avoiding the side effects of systemic administration and having a minor impact on BG levels,providing a potentially effective treatment for patients with diabetes[12].However,there is limited clinical evidence to compare the efficacy and glycaemic effects of retroauricular subperiosteal injection vs systemic intravenous GC administration in patients with SD and diabetes.This study aimed to explore the efficacy of retroauricular subperiosteal injection and systemic intravenous GC administration for treating patients with SD and DM and their effects on BG,providing a safer and more effective clinical treatment approach.展开更多
Although digital changes in power systems have added more ways to monitor and control them,these changes have also led to new cyber-attack risks,mainly from False Data Injection(FDI)attacks.If this happens,the sensors...Although digital changes in power systems have added more ways to monitor and control them,these changes have also led to new cyber-attack risks,mainly from False Data Injection(FDI)attacks.If this happens,the sensors and operations are compromised,which can lead to big problems,disruptions,failures and blackouts.In response to this challenge,this paper presents a reliable and innovative detection framework that leverages Bidirectional Long Short-Term Memory(Bi-LSTM)networks and employs explanatory methods from Artificial Intelligence(AI).Not only does the suggested architecture detect potential fraud with high accuracy,but it also makes its decisions transparent,enabling operators to take appropriate action.Themethod developed here utilizesmodel-free,interpretable tools to identify essential input elements,thereby making predictions more understandable and usable.Enhancing detection performance is made possible by correcting class imbalance using Synthetic Minority Over-sampling Technique(SMOTE)-based data balancing.Benchmark power system data confirms that the model functions correctly through detailed experiments.Experimental results showed that Bi-LSTM+Explainable AI(XAI)achieved an average accuracy of 94%,surpassing XGBoost(89%)and Bagging(84%),while ensuring explainability and a high level of robustness across various operating scenarios.By conducting an ablation study,we find that bidirectional recursive modeling and ReLU activation help improve generalization and model predictability.Additionally,examining model decisions through LIME enables us to identify which features are crucial for making smart grid operational decisions in real time.The research offers a practical and flexible approach for detecting FDI attacks,improving the security of cyber-physical systems,and facilitating the deployment of AI in energy infrastructure.展开更多
A security issue with multi-sensor unmanned aerial vehicle(UAV)cyber physical systems(CPS)from the viewpoint of a false data injection(FDI)attacker is investigated in this paper.The FDI attacker can employ attacks on ...A security issue with multi-sensor unmanned aerial vehicle(UAV)cyber physical systems(CPS)from the viewpoint of a false data injection(FDI)attacker is investigated in this paper.The FDI attacker can employ attacks on feedback and feed-forward channels simultaneously with limited resource.The attacker aims at degrading the UAV CPS's estimation performance to the max while keeping stealthiness characterized by the Kullback-Leibler(K-L)divergence.The attacker is resource limited which can only attack part of sensors,and the attacked sensor as well as specific forms of attack signals at each instant should be considered by the attacker.Also,the sensor selection principle is investigated with respect to time invariant attack covariances.Additionally,the optimal switching attack strategies in regard to time variant attack covariances are modeled as a multi-agent Markov decision process(MDP)with hybrid discrete-continuous action space.Then,the multi-agent MDP is solved by utilizing the deep Multi-agent parameterized Q-networks(MAPQN)method.Ultimately,a quadrotor near hover system is used to validate the effectiveness of the results in the simulation section.展开更多
Cinobufacini, a compound extracted from bufo toad, is a traditional Chinese medicine(TCM) that has been shown to have efficacy in cancer treatment.The cinobufacini injection is widely used in patients with moderate an...Cinobufacini, a compound extracted from bufo toad, is a traditional Chinese medicine(TCM) that has been shown to have efficacy in cancer treatment.The cinobufacini injection is widely used in patients with moderate and advanced liver cancer in China.In the present study, we aimed to determine the effects of cinobufacini injection for this specific category of patients.A search for randomized control trials(RCTs) using cinobufacini was conducted in the PubMed, EMBASE, CENTRAL, and the other four major Chinese databases.The systematic review was performed according to the recommendations of the Cochrane collaboration, and the RevMan 5.3.software was used for statistical analysis.A random-effects model was used to perform the data.Risk ratios(RR) with corresponding 95% confidence interval were calculated according to Cochrane handbook.A total of 11 RCTs consisting of 728 patients were included.All of the trials demonstrated significantly improved total response rates, total response rates of Karnofsky Performance Score(KPS), 1-to 2-year survival rates, and quality of life in the intervention groups injected with cinobufacini.There was no statistically significant difference between the groups(cinobufacini versus no cinobufacini; cinobufacini plus transcatheter arterial chemoembolization(TACE) versus TACE only) in terms of the 6-month survival rate, clinical benefit rate and clinical benefit rate of KPS.This systematic review demonstrated the beneficial effects of cinobufacini injection in terms of total response rate and the 1-to 2-year survival rate in patients with moderate and advanced liver cancer.The efficacy for the cinobufacini injection group might be better than that of the control group for treatment of moderate and advanced liver cancer.Given that the majority of the trials were of low quality, more high-quality prospective RCTs with strict design in accordance with CONSORT for TCM are needed.展开更多
A simple, rapid and sensitive flow injection chemiluminescence （FI-CL） method has been developed for the determination of meloxicam. The method is based on the CL-emitting reaction between meloxicam and potassium pe...A simple, rapid and sensitive flow injection chemiluminescence （FI-CL） method has been developed for the determination of meloxicam. The method is based on the CL-emitting reaction between meloxicam and potassium permanganate in a hydrochloric acid medium, enhanced by formaldehyde （HCHO）. Under optimum conditions, calibration curve over the range of 1.0-20.0μg/mL was obtained. The proposed method was successfully applied to the determination of meloxicam in capsules with no evi- dence of interference from common excipients. The detection limit of this method was 25.6 ng/mL. The relative standard deviation was 2.1% for 10.0 μg/mL meloxicam. The sample throughput was found to be 120 samples/h.展开更多
[Objective] This study aimed to investigate the feasibility of transgenesis by injecting exogenous DNA into zygote cytoplasm of Buffalo. [Method] Buffalo oocytes were randomly divided into two groups 20-22 h after in ...[Objective] This study aimed to investigate the feasibility of transgenesis by injecting exogenous DNA into zygote cytoplasm of Buffalo. [Method] Buffalo oocytes were randomly divided into two groups 20-22 h after in vitro maturation. One group of oocytes was introduced with about 7.5 pl of 50 μg/ml DNA solution containing linear EGFP fragment by cytoplasmic injection 7-10 h or 18-20 h after in vitro fertilization （IVF）; the other group of oocytes was introduced with mixture of a single buffalo sperm and about 7.5 pl of 50 μg/ml DNA solution containing linear EGFP fragment by cytoplasmic injection （generally called ICSI-Mediated Gene Transfer, ICSI-Tr）. Expression of exogenous DNA was observed and recorded during the process of embryonic development. [Result] Early embryonic gene expression efficiency and blastocyst gene expression efficiency in IVF injection group showed no significant difference compared with that in ICSI-Tr group （P0.05）. In addition, the cleavage rate and early embryonic gene expression efficiency in IVF injection group were significantly higher with injection at 7-10 h post IVF than that at 18-20 h post IVF （P0.05）. [Conclusion] These results indicate that transgenic buffalo embryos can be generated by injecting exogenous DNA into cytoplasm of IVF oocytes, and the optimal injection time is 7-10 h post IVF.展开更多
On the basis of detailed analysis of a novel harmonic counteracting method which can be used to effectively compensate the supply line harmonic currents of a passive single phase diode bridge rectifier, this paper pr...On the basis of detailed analysis of a novel harmonic counteracting method which can be used to effectively compensate the supply line harmonic currents of a passive single phase diode bridge rectifier, this paper presents two simpler single phase diode bridge rectifier configurations and their alternatives which can achieve low supply line current THD(total harmonic distortion) too. Moreover, this paper also proposes a few passive hamonic counteracting networks for multi single phase rectifiers which are connected in parallel.展开更多
BACKGROUND The previous studies have primarily focused on the influence of botulinum toxin A(BoNT-A)injection on emotions during the period of peak motor symptom improvement in blepharospasm patients,based on facial f...BACKGROUND The previous studies have primarily focused on the influence of botulinum toxin A(BoNT-A)injection on emotions during the period of peak motor symptom improvement in blepharospasm patients,based on facial feedback hypothesis.AIM To evaluate the sustained anxiolytic and antidepressant effects of BoNT-A in blepharospasm patients beyond motor symptom control.METHODS We recruited benign essential blepharospasm patients with BoNT-A treatment and collected their data to compare scale scores of Jankovic Rating Scale,Blepharospasm Disability Index,Self-rating Anxiety Scale(SAS),Self-rating Depression Scale(SDS),Hamilton Anxiety Scale and Hamilton Depression Scale between pretreatment(baseline)and pre-reinjection(treatment),to further assess the effects of repeated treatments with BoNT by using sub-group analyses in the certain special states.RESULTS A total of 21 eligible blepharospasm patients were with the mean age of 58.4 years and a male-to-female ratio of 1:6.Significantly decreases in the subscale scores of SDS and SAS,including SDS well-being index,decreased capacity and hard to decide,SAS inability to sit still and headache were showed at post-a single BoNT-A injection when scale scores of Jankovic Rating Scale and Blepharospasm Disability Index were matched between baseline and posttreatment.With each additional BoNT-A injection,the odds ratio of patients with the moderate depressive symptoms decreased by 92.6%.Moreover,BoNT treatment remained a decrease in the subscale scores of SDS and SAS in patients with repeated injections.CONCLUSION This study is to demonstrate that repeated BoNT-A injection have a long-lasting relief for anxiety and depressive symptoms in blepharospasm even after its motor symptom-modulating effects have diminished.展开更多
Rationale:This case report describes a couple with recurrent fertilization failure despite undergoing multiple cycles of intracytoplasmic sperm injection(ICSI).The principal clinical concern was suspected oocyte activ...Rationale:This case report describes a couple with recurrent fertilization failure despite undergoing multiple cycles of intracytoplasmic sperm injection(ICSI).The principal clinical concern was suspected oocyte activation deficiency(OAD),in which fertilization is impeded due to the oocyte’s inability to initiate embryogenesis,commonly attributed to inadequate intracellular calcium(Ca^(2+))release following sperm injection.Patient concerns:The couple repeatedly experienced complete or near-complete fertilization failure in previous ICSI cycles,raising suspicion of an underlying oocyte activation defect.Diagnosis:Based on the repeated absence of fertilization post-ICSI and clinical history,a diagnosis of suspected OAD leading to recurrent ICSI fertilization failure was considered.Interventions:Artificial oocyte activation(AOA)using the calcium ionophore A23187 was performed.After ICSI,unfertilized oocytes were exposed to the ionophore to induce Ca^(2+)influx,simulating physiological calcium oscillations essential for oocyte activation.The efficacy of intervention was evaluated through subsequent embryonic development,morphological grading,and chromosomal integrity.Outcomes:Following AOA treatment,successful oocyte activation occurred,resulting in the formation of high-grade embryos with normal developmental progression.Chromosomal analysis revealed no detectable abnormalities,indicating genomic stability.Lessons:Calcium ionophore–mediated AOA may serve as an effective adjunct in cases of recurrent ICSI failure attributed to OAD.This case highlights the importance of individualized therapeutic strategies in assisted reproduction;however,further research is needed to refine protocols,validate broader clinical efficacy,and assess long-term safety,including potential epigenetic risks.展开更多
OBJECTIVE:To evaluate systematically the efficacy and safety of Danshenchuanxiongqin Injection(DCI)in the treatment of unstable angina pectoris(UAP).METHODS:Randomized controlled trials(RCTs)regarding DCI used for tre...OBJECTIVE:To evaluate systematically the efficacy and safety of Danshenchuanxiongqin Injection(DCI)in the treatment of unstable angina pectoris(UAP).METHODS:Randomized controlled trials(RCTs)regarding DCI used for treating UAP were searched in English and Chinese electronic databases from inception to January 2014.Two reviewers independently retrieved RCTs and extracted relevant information.The Cochrane risk of bias method was used to assess the quality of included studies,and a Meta-analysis was conducted with Review Manager5.2 software.RESULTS:Eleven RCTs involving 1034 participants were included.The methodological quality was relatively passable.The Meta-analysis indicated that the combined use of DCI and conventional treatment with Western Medicine(WM)was more efficacious in the outcomes of total effective rate[Relative Risk(RR)=1.27,95%CI(confidence interval;1.18,1.35),P<0.000 01],the total effective rate of ECG[RR=1.40,95%CI(1.18,1.66),P<0.000 01],total cholesterol[Mean difference(MD)=-0.58,95%CI(-0.83,-0.33),P<0.000 01],total triglycerides[MD=-0.36,95%CI(-0.54,-0.17),P=0.0001],and the number of ST-segment depression[MD=-0.36,95%CI(-0.54,-0.17),P=0.0001].There were two adverse drug reactions reported in one study.CONCLUSION:Based on the systematic review,DCI combined with WM appeared to be efficacious in the treatment UAP.However,the evidence of DCI for treating UAP requires large-scale and double-blind RCTs to substantiate these findings.展开更多
文摘After many years of development, die casting technology of metallic materials has been matured. In this paper,the lower-support and its injecting system were created with commercial software. And then the simulation softwareFLOW3D was applied to study the flow behavior of the melt during injection filling process. Both temperature field anddefect distribution were simulated. Based on these results, the better injecting system with two additional overflows wasadopted.
基金supported by Basic and Applied Basic research foundation of Guangdong province(Nos.2021A1515010343 and 2022A1515011582)the Science and Technology Program of Guangdong Province(Nos.2021A0505030026 and 2022A0505050029).
文摘In the scenario of a steam generator tube rupture accident in a lead-cooled fast reactor,secondary circuit subcooled water under high pressure is injected into an ordinary-pressure primary vessel,where a molten lead-based alloy(typically pure lead or lead-bismuth eutectic(LBE))is used as the coolant.To clarify the pressure build-up characteristics under water-jet injection,this study conducted several experiments by injecting pressurized water into a molten LBE pool at Sun Yat-sen University.To obtain a further understanding,several new experimental parameters were adopted,including the melt temperature,water subcooling,injection pressure,injection duration,and nozzle diameter.Through detailed analyses,it was found that the pressure and temperature during the water-melt interaction exhibited a consistent variation trend with our previous water-droplet injection mode LBE experiment.Similarly,the existence of a steam explosion was confirmed,which typically results in a much stronger pressure build-up.For the non-explosion cases,increasing the injection pressure,melt-pool temperature,nozzle diameter,and water subcooling promoted pressure build-up in the melt pool.However,a limited enhancement effect was observed when increasing the injection duration,which may be owing to the continually rising pressure in the interaction vessel or the isolation effect of the generated steam cavity.Regardless of whether a steam explosion occurred,the calculated mechanical and kinetic energy conversion efficiencies of the melt were relatively small(not exceeding 4.1%and 0.7%,respectively).Moreover,the range of the conversion efficiency was similar to that of previous water-droplet experiments,although the upper limit of the jet mode was slightly lower.
基金The Natural Science Foundation of Joint Research Project of the China Association of Chinese Medicine(Grant No.2024-LHGG-YSBZ2004)。
文摘Sepsis-associated acute kidney injury(SA-AKI)represents a leading contributor to the elevated mortality observed in sepsis patients.In the present study,we conducted a comprehensive meta-analysis to assess the clinical effectiveness of continuous renal replacement therapy(CRRT)in combination with Xuebijing injection for the treatment of SA-AKI.A systematic search was performed across major databases,including CNKI,VIP,Sinomed,WanFang,PubMed,and the Cochrane Library,covering publications up to January 2025.Quality assessment and statistical analyses were carried out using RevMan 5.4.The pooled results demonstrated that the adjunctive use of Xuebijing injection with CRRT,as compared to CRRT alone,yielded significantly better outcomes.Specifically,this combined therapy was associated with enhanced recovery of renal function,more effective clearance of inflammatory mediators,improved immune modulation,better regulation of coagulation parameters,and a marked reduction in mortality among SA-AKI patients.
基金supported in part by the National Science Foundation of China(62373240,62273224,U24A20259).
文摘Dear Editor,The attacker is always going to intrude covertly networked control systems(NCSs)by dynamically changing false data injection attacks(FDIAs)strategy,while the defender try their best to resist attacks by designing defense strategy on the basis of identifying attack strategy,maintaining stable operation of NCSs.To solve this attack-defense game problem,this letter investigates optimal secure control of NCSs under FDIAs.First,for the alterations of energy caused by false data,a novel attack-defense game model is constructed,which considers the changes of energy caused by the actions of the defender and attacker in the forward and feedback channels.
基金Supported by Hospital-level Project of Shiyan Taihe Hospital(2019JJXM117).
文摘[Objectives]To investigate the clinical efficacy of acupoint injection of nerve growth factors combined with task-oriented training for patients with post-stroke hemiplegic gait,and quantitatively evaluate the outcomes using the Gait Watch analysis system.[Methods]A total of 90 patients with post-stroke hemiplegia,who were hospitalized at the Rehabilitation Center of Taihe Hospital between January 2023 and December 2023,were selected for this study.The participants were randomly assigned to three groups:the task-oriented rehabilitation training group(control group I,n=30),the ordinary acupuncture combined with task-oriented training group(control group II,n=30),and the acupoint injection combined with task-oriented training group(observation group,n=30).Each group underwent treatment for 4 weeks.The Gait Watch analysis system was employed to assess the spatiotemporal gait parameters of the patients prior to treatment,as well as 2 weeks post treatment and 4 weeks post treatment.The efficacy of the treatment was subsequently analyzed.[Results]After 4 weeks of treatment,the spatiotemporal gait parameters,specifically step length,step speed,step frequency,percentage of the standing phase,and percentage of the swinging phase,exhibited significant improvement in the observation group compared to those before treatment(P<0.05).Furthermore,the degree of improvement in the observation group was superior to that observed in both control group I and control group II,with the differences reaching statistical significance(P<0.05).[Conclusions]Acupoint injection combined with task-oriented training has been shown to significantly enhance gait function in patients with post-stroke hemiplegia.The Gait Watch analysis system offers an accurate and objective quantitative assessment,making it a valuable tool for clinical application and promotion.
基金funded by the Joint Funds of the National Natural Science Foundation of China(U23A20671)the Major Project of Inner Mongolia Science and Technology(2021ZD0034)the Open Research Fund of State Key Laboratory of Geomechanics and Geotechnical Engineering(No.Z021003)。
文摘The occurrence time and magnitude of injection-induced seismicity are influenced by engineering factors,such as wellhead pressure,injection location,injection volume,and injection rate.Understanding the relationship between injection operations and seismic magnitude is of great significance for optimizing industrial production and reducing earthquake disasters.Numerical simulation of hydromechanical coupling is a crucial method for studying injection-induced seismicity.However,few studies have explored the risk management measures for injection-induced seismicity from the perspective of engineering.How seismic magnitudes can be reduced through reasonable adjustments to injection operations in engineering remains unclear.Therefore,in this study,a 3D hydro-mechanical coupling model involving multiple faults and injection wells was established based on the geological background and well location of Fox Creek,Canada.Different injection schemes under multi-well and multi-fault conditions were studied,and a traffic light system was used to simulate and control the magnitudes under a multi-well injection scheme.Specifically,we simulated injection scenarios involving up to three wells and analyzed the response of five faults.We compared the maximum moment magnitude of different scenarios by controlling the same injection volume.The results revealed the effect and advantage of the multi-well scheme in reducing seismic magnitude.To reduce the risk of induced seismicity,utilizing far-fault operational wells to compensate for the effects of near-fault operational wells proves to be an efficient and cost-effective method,with potential for wide practical applications.
文摘BACKGROUND Sub-acromial injections are a therapeutic option for rotator cuff injuries;however,evidence regarding the most effective drug in this context is unclear,which needs to be investigated.AIM To evaluate the effectiveness of various sub-acromial injections for rotator cuff injuries.METHODS We conducted a systematic review and pair-wise and network meta-analyses of randomized clinical trials(RCTs)comparing sub-acromial injections for rotator cuff injuries.The interventions evaluated were hyaluronic acid(HA),platelet-rich plasma(PRP),prolotherapy,and corticosteroids.The outcomes of interest were pain and functional improvement,which were evaluated with standardized scores.The Risk of Bias 2 tool and the Grading of Recommendations,Assessment,Development and Evaluation methodology were used to assess data quality.RESULTS Twenty RCTs,comprising 1479 participants,were included.In the short term,HA achieved the best outcomes[pain mean difference(MD)=-1.48,95%confidence interval(CI)-2.37 to-0.59;function MD=10.18,95%CI:4.96-15.41].In the medium term,HA,PRP,HA+PRP,and corticosteroids were not superior to placebo in improving pain.Based on function,HA+PRP was superior to placebo,corticosteroids,and PRP(MD=26.72;95%CI:8.02-45.41).In the long term,HA,PRP,and corticosteroids were not superior to placebo in reducing pain.However,based on function,HA+PRP,PRP,and HA were superior to placebo,and HA+PRP had the best result(MD=36.64;95%CI:31.66-33.62).CONCLUSION HA provides satisfactory short-term results,while HA with PRP demonstrates functional improvement in the medium and long terms.However,no intervention maintained the pain-relief effect on>3-month follow-up.
基金the National Nature Science Foundation of China(Grant Nos.42077435 and 42377171).
文摘This study elucidates the findings of a computational investigation into the stimulation characteristics of natural reservoir systems enhanced by high-voltage electropulse-assisted fluid injection.The presented methodology delineates the comprehensive rock-fracturing process induced by electropulse and subsequent fluid injection,encompassing the discharge circuit,plasma channel formation,shockwave propagation,and hydro-mechanical response.A hydromechanical model incorporating an anisotropic plastic damage constitutive law,discrete fracture networks,and heterogeneous distribution is developed to represent the natural reservoir system.The results demonstrate that high-voltage electropulse effectively generates intricate fracture networks,significantly enhances the hydraulic properties of reservoir systems,and mitigates the adverse impact of ground stress on fracturing.The stimulationenhancing effect of electropulse is observed to intensify with increasing discharge voltage,with enhancements of 118.0%,139.5%,and 169.0%corresponding to discharge voltages of 20 kV,40 kV,and 60 kV,respectively.Additionally,a high-voltage electropulse with an initial voltage of U_(0)=80 kV and capacitance C=5μF has been shown to augment the efficiency of injection activation to approximately 201.1%compared to scenarios without electropulse.Under the influence of high-voltage electropulse,the fluid pressure distribution diverges from the conventional single direction of maximum stress,extending over larger areas.These innovative methods and findings hold potential implications for optimizing reservoir stimulation in geo-energy engineering.
文摘Background Sudden sensorineural hearing loss(SSNHL)is a rapid condition of hearing impairment,typically occurring over 72 hours and affecting the inner ear or auditory nerve pathways.The etiology of SSNHL remains largely idiopathic,with potential causes including viral infections,vascular occlusions,and autoimmune disorders.Traditional treatment primarily involves systemic corticosteroids,but their efficacy is inconsistent,leading to exploring alternative and adjunctive therapies such as intratympanic steroid(ITS)injections.Objective This Systematic Review and Meta-Analysis intends to determine the effectiveness of ITS injections as an initial treatment,combined therapy,or salvage treatment for SSNHL compared to systemic steroids alone.Methods An inclusive study was performed through the PubMed,Scopus,Web of Science,and Cochrane Library databases,encompassing publications published from 2015 to 2024.Case-control studies,cohort studies,randomized controlled trials(RCTs),and systematic reviews were included.Two separate reviewers read the studies and extracted data on steroid administration methods,study outcomes,and risk of bias(ROB)using the Cochrane Collaboration Tool and RevMan 5.4.Results The review included 12 studies with varied designs,including prospective RCTs,systematic reviews,and retrospective analyses.Combined ITS and systemic steroid therapy showed superior hearing recovery compared to systemic steroids alone in several studies.ITS alone demonstrated variable efficacy,with some studies indicating benefits,particularly as a salvage therapy.ROB assessment revealed variability in methodological rigor,with studies like those of Li&Ding et al.(2020)and Devantier et al.(2022)showing low risk,while others exhibited higher risks,particularly in random sequence generation and allocation concealment.Conclusion The findings suggest that ITS injections,particularly when combined with systemic steroids,can enhance hearing recovery in SSNHL patients.However,variability in study outcomes and methodological quality underscores the need for standardized protocols and further high-quality RCTs.This analysis underscores the prospective advantages of ITS therapy while stressing the need for stringent study designs to enhance SSNHL treatment methodologies.
基金supported by the National Natural Science Foundation of China(No.52175284)the National Key Research and Development Program of China(Grant No.2022YFB3404201).
文摘This study investigates the formation feasibility of the integrated bottom car body components with dual die casting injection molding technology.During the production of a die-cast super-large one-piece body part weighing over 10,000 t,a thorough comparison and investigation were conducted on the arising issues,using both single and double injection systems.Particular attention was given to meticulously discussing the die casting filling problems and microstructural defects that originated from the filling process.The research findings indicate that the implementation of a double injection system can significantly minimize cold shuts and reduce the solidification time.The effectiveness of this die casting technique was further confirmed by the production of high-quality castings using a scaled model that replicated real casting conditions at a 1:3 ratio,thereby maintaining a one-to-one correspondence in essential aspects.This successful study offers both theoretical insights and practical applications for the production of integrated bottom car bodies utilizing die casting in conjunction with a dual injection system.
文摘BACKGROUND Managing sudden deafness(SD)in patients with diabetes mellitus(DM)is partic-ularly challenging due to the heightened risk of adverse effects associated with systemic drug administration.This study explores the potential of retroauricular subperiosteal injection as a localized drug delivery method for a more effective and safe treatment.AIM To compare the efficacy of retroauricular subperiosteal injection vs systemic intravenous glucocorticoid(GC)administration for SD in patients with DM and assess the effects on blood glucose levels.METHODS A total of 128 cases of type 2 DM(T2DM)with SD diagnosed and treated in Zibo Central Hospital from February 2021 to July 2023 were divided into two groups:An observation group(66 cases receiving retroauricular subperiosteal injection of methylprednisolone)and a control group(62 cases receiving systemic intravenous administration of methylprednisolone).The two groups were compared in terms of therapeutic efficacy,hearing recovery,blood glucose level changes,and incidence of adverse reactions.Binary logistic regression was used to analyze the factors affecting therapeutic efficacy.RESULTS The observation group showed a significantly higher total effective rate(90.91%)compared with the control group(75.81%,P<0.05).Additionally,pure-tone hearing threshold,fasting plasma glucose,and 2-hour postprandial blood glucose were significantly lower in the observation group compared with the control group(P<0.05).The incidence of adverse reactions was also lower in the observation group than in the control group(7.58%vs 22.58%,P<0.05).A T2DM course longer than 5 years and systemic intravenous GC administration were identified as independent risk factors for treatment inefficacy(P<0.05).INTRODUCTION Sudden deafness(SD)is a clinical emergency characterized by rapid-onset hearing loss that is often accompanied by clinical symptoms such as tinnitus and vertigo[1].Although its pathogenesis remains unclear,it is supposedly associated with factors,including inner ear microcirculation disorders,autoimmune diseases,and viral infections[2,3].Patients with diabetes are particularly susceptible to microvascular complications due to poor long-term glycemic control,affecting the ear’s microcirculation,subsequently increasing the risk of SD[4].Type 2 diabetes mellitus(T2DM),a chronic metabolic disease,causes multiple microvascular damages throughout the body,complicating SD treatment in patients with diabetes[5,6].Inner ear microcirculation disturbances in patients with diabetes may exacerbate the risk of SD,and its pathological process may be related to vascular endothelial dysfunction,inflammatory reactions,and hemorrhological changes caused by diabetes mellitus(DM)[7].Current SD treatments include glucocorticoids(GCs),vasodilators,and hyperbaric oxygen therapy[8].GCs are widely used due to their anti-inflammatory and immunosuppressive effects[9].However,systemic GCs may cause blood glucose(BG)fluctuations and even increase the risk of complications in patients with DM,limiting their clinical use in this population[10].Therefore,there is a compelling and immediate need for a local alternative solution that minimally affects the metabolic mechanisms.In recent years,retroauricular subperiosteal injection has emerged as a localized administration modality for treating SD[11].This method allows drugs to directly act on the inner ear,avoiding the side effects of systemic administration and having a minor impact on BG levels,providing a potentially effective treatment for patients with diabetes[12].However,there is limited clinical evidence to compare the efficacy and glycaemic effects of retroauricular subperiosteal injection vs systemic intravenous GC administration in patients with SD and diabetes.This study aimed to explore the efficacy of retroauricular subperiosteal injection and systemic intravenous GC administration for treating patients with SD and DM and their effects on BG,providing a safer and more effective clinical treatment approach.
基金the Deanship of Scientific Research and Libraries in Princess Nourah bint Abdulrahman University for funding this research work through the Research Group project,Grant No.(RG-1445-0064).
文摘Although digital changes in power systems have added more ways to monitor and control them,these changes have also led to new cyber-attack risks,mainly from False Data Injection(FDI)attacks.If this happens,the sensors and operations are compromised,which can lead to big problems,disruptions,failures and blackouts.In response to this challenge,this paper presents a reliable and innovative detection framework that leverages Bidirectional Long Short-Term Memory(Bi-LSTM)networks and employs explanatory methods from Artificial Intelligence(AI).Not only does the suggested architecture detect potential fraud with high accuracy,but it also makes its decisions transparent,enabling operators to take appropriate action.Themethod developed here utilizesmodel-free,interpretable tools to identify essential input elements,thereby making predictions more understandable and usable.Enhancing detection performance is made possible by correcting class imbalance using Synthetic Minority Over-sampling Technique(SMOTE)-based data balancing.Benchmark power system data confirms that the model functions correctly through detailed experiments.Experimental results showed that Bi-LSTM+Explainable AI(XAI)achieved an average accuracy of 94%,surpassing XGBoost(89%)and Bagging(84%),while ensuring explainability and a high level of robustness across various operating scenarios.By conducting an ablation study,we find that bidirectional recursive modeling and ReLU activation help improve generalization and model predictability.Additionally,examining model decisions through LIME enables us to identify which features are crucial for making smart grid operational decisions in real time.The research offers a practical and flexible approach for detecting FDI attacks,improving the security of cyber-physical systems,and facilitating the deployment of AI in energy infrastructure.
文摘A security issue with multi-sensor unmanned aerial vehicle(UAV)cyber physical systems(CPS)from the viewpoint of a false data injection(FDI)attacker is investigated in this paper.The FDI attacker can employ attacks on feedback and feed-forward channels simultaneously with limited resource.The attacker aims at degrading the UAV CPS's estimation performance to the max while keeping stealthiness characterized by the Kullback-Leibler(K-L)divergence.The attacker is resource limited which can only attack part of sensors,and the attacked sensor as well as specific forms of attack signals at each instant should be considered by the attacker.Also,the sensor selection principle is investigated with respect to time invariant attack covariances.Additionally,the optimal switching attack strategies in regard to time variant attack covariances are modeled as a multi-agent Markov decision process(MDP)with hybrid discrete-continuous action space.Then,the multi-agent MDP is solved by utilizing the deep Multi-agent parameterized Q-networks(MAPQN)method.Ultimately,a quadrotor near hover system is used to validate the effectiveness of the results in the simulation section.
基金Science and Technology Project of Putian University,China(Grant No.2013035)
文摘Cinobufacini, a compound extracted from bufo toad, is a traditional Chinese medicine(TCM) that has been shown to have efficacy in cancer treatment.The cinobufacini injection is widely used in patients with moderate and advanced liver cancer in China.In the present study, we aimed to determine the effects of cinobufacini injection for this specific category of patients.A search for randomized control trials(RCTs) using cinobufacini was conducted in the PubMed, EMBASE, CENTRAL, and the other four major Chinese databases.The systematic review was performed according to the recommendations of the Cochrane collaboration, and the RevMan 5.3.software was used for statistical analysis.A random-effects model was used to perform the data.Risk ratios(RR) with corresponding 95% confidence interval were calculated according to Cochrane handbook.A total of 11 RCTs consisting of 728 patients were included.All of the trials demonstrated significantly improved total response rates, total response rates of Karnofsky Performance Score(KPS), 1-to 2-year survival rates, and quality of life in the intervention groups injected with cinobufacini.There was no statistically significant difference between the groups(cinobufacini versus no cinobufacini; cinobufacini plus transcatheter arterial chemoembolization(TACE) versus TACE only) in terms of the 6-month survival rate, clinical benefit rate and clinical benefit rate of KPS.This systematic review demonstrated the beneficial effects of cinobufacini injection in terms of total response rate and the 1-to 2-year survival rate in patients with moderate and advanced liver cancer.The efficacy for the cinobufacini injection group might be better than that of the control group for treatment of moderate and advanced liver cancer.Given that the majority of the trials were of low quality, more high-quality prospective RCTs with strict design in accordance with CONSORT for TCM are needed.
文摘A simple, rapid and sensitive flow injection chemiluminescence （FI-CL） method has been developed for the determination of meloxicam. The method is based on the CL-emitting reaction between meloxicam and potassium permanganate in a hydrochloric acid medium, enhanced by formaldehyde （HCHO）. Under optimum conditions, calibration curve over the range of 1.0-20.0μg/mL was obtained. The proposed method was successfully applied to the determination of meloxicam in capsules with no evi- dence of interference from common excipients. The detection limit of this method was 25.6 ng/mL. The relative standard deviation was 2.1% for 10.0 μg/mL meloxicam. The sample throughput was found to be 120 samples/h.
基金Supported by National High Technology Research and Development (863) Program of China (2011AA100607)National Transgenic Major Project of China (2010ZX08007003)~~
文摘[Objective] This study aimed to investigate the feasibility of transgenesis by injecting exogenous DNA into zygote cytoplasm of Buffalo. [Method] Buffalo oocytes were randomly divided into two groups 20-22 h after in vitro maturation. One group of oocytes was introduced with about 7.5 pl of 50 μg/ml DNA solution containing linear EGFP fragment by cytoplasmic injection 7-10 h or 18-20 h after in vitro fertilization （IVF）; the other group of oocytes was introduced with mixture of a single buffalo sperm and about 7.5 pl of 50 μg/ml DNA solution containing linear EGFP fragment by cytoplasmic injection （generally called ICSI-Mediated Gene Transfer, ICSI-Tr）. Expression of exogenous DNA was observed and recorded during the process of embryonic development. [Result] Early embryonic gene expression efficiency and blastocyst gene expression efficiency in IVF injection group showed no significant difference compared with that in ICSI-Tr group （P0.05）. In addition, the cleavage rate and early embryonic gene expression efficiency in IVF injection group were significantly higher with injection at 7-10 h post IVF than that at 18-20 h post IVF （P0.05）. [Conclusion] These results indicate that transgenic buffalo embryos can be generated by injecting exogenous DNA into cytoplasm of IVF oocytes, and the optimal injection time is 7-10 h post IVF.
文摘On the basis of detailed analysis of a novel harmonic counteracting method which can be used to effectively compensate the supply line harmonic currents of a passive single phase diode bridge rectifier, this paper presents two simpler single phase diode bridge rectifier configurations and their alternatives which can achieve low supply line current THD(total harmonic distortion) too. Moreover, this paper also proposes a few passive hamonic counteracting networks for multi single phase rectifiers which are connected in parallel.
基金Supported by the Special Funds of Jiangsu Provincial Key Research and Development Projects,No.BE2019612Scientific Research Project Cooperated by Lanzhou Biotechnology Development Co.,Ltd.+3 种基金the Key R&D Program of Jiangsu Science and Technology Project,No.BE2022049 and No.BE2022049-1National Natural Science Foundation of China,No.82171249Nanjing Rehabilitation Medicine Center ProjectJiangsu Provincial Health Commission Special Fund for Aging and Health.
文摘BACKGROUND The previous studies have primarily focused on the influence of botulinum toxin A(BoNT-A)injection on emotions during the period of peak motor symptom improvement in blepharospasm patients,based on facial feedback hypothesis.AIM To evaluate the sustained anxiolytic and antidepressant effects of BoNT-A in blepharospasm patients beyond motor symptom control.METHODS We recruited benign essential blepharospasm patients with BoNT-A treatment and collected their data to compare scale scores of Jankovic Rating Scale,Blepharospasm Disability Index,Self-rating Anxiety Scale(SAS),Self-rating Depression Scale(SDS),Hamilton Anxiety Scale and Hamilton Depression Scale between pretreatment(baseline)and pre-reinjection(treatment),to further assess the effects of repeated treatments with BoNT by using sub-group analyses in the certain special states.RESULTS A total of 21 eligible blepharospasm patients were with the mean age of 58.4 years and a male-to-female ratio of 1:6.Significantly decreases in the subscale scores of SDS and SAS,including SDS well-being index,decreased capacity and hard to decide,SAS inability to sit still and headache were showed at post-a single BoNT-A injection when scale scores of Jankovic Rating Scale and Blepharospasm Disability Index were matched between baseline and posttreatment.With each additional BoNT-A injection,the odds ratio of patients with the moderate depressive symptoms decreased by 92.6%.Moreover,BoNT treatment remained a decrease in the subscale scores of SDS and SAS in patients with repeated injections.CONCLUSION This study is to demonstrate that repeated BoNT-A injection have a long-lasting relief for anxiety and depressive symptoms in blepharospasm even after its motor symptom-modulating effects have diminished.
文摘Rationale:This case report describes a couple with recurrent fertilization failure despite undergoing multiple cycles of intracytoplasmic sperm injection(ICSI).The principal clinical concern was suspected oocyte activation deficiency(OAD),in which fertilization is impeded due to the oocyte’s inability to initiate embryogenesis,commonly attributed to inadequate intracellular calcium(Ca^(2+))release following sperm injection.Patient concerns:The couple repeatedly experienced complete or near-complete fertilization failure in previous ICSI cycles,raising suspicion of an underlying oocyte activation defect.Diagnosis:Based on the repeated absence of fertilization post-ICSI and clinical history,a diagnosis of suspected OAD leading to recurrent ICSI fertilization failure was considered.Interventions:Artificial oocyte activation(AOA)using the calcium ionophore A23187 was performed.After ICSI,unfertilized oocytes were exposed to the ionophore to induce Ca^(2+)influx,simulating physiological calcium oscillations essential for oocyte activation.The efficacy of intervention was evaluated through subsequent embryonic development,morphological grading,and chromosomal integrity.Outcomes:Following AOA treatment,successful oocyte activation occurred,resulting in the formation of high-grade embryos with normal developmental progression.Chromosomal analysis revealed no detectable abnormalities,indicating genomic stability.Lessons:Calcium ionophore–mediated AOA may serve as an effective adjunct in cases of recurrent ICSI failure attributed to OAD.This case highlights the importance of individualized therapeutic strategies in assisted reproduction;however,further research is needed to refine protocols,validate broader clinical efficacy,and assess long-term safety,including potential epigenetic risks.
基金Supported by National Natural Science Foundation of China(Study on the Key Factors of Allergic Reaction that Caused by Chinese Herbal Injection Based on the Three-Dimensional Database and Multi-Dimensional Data-Mining,No.81473547)Scientific Research Innovation Team Project of Beijing University of Chinese Medicine(the Basic Theory of Traditional Chinese Medicine,No.2011-CXTD-14)
文摘OBJECTIVE:To evaluate systematically the efficacy and safety of Danshenchuanxiongqin Injection(DCI)in the treatment of unstable angina pectoris(UAP).METHODS:Randomized controlled trials(RCTs)regarding DCI used for treating UAP were searched in English and Chinese electronic databases from inception to January 2014.Two reviewers independently retrieved RCTs and extracted relevant information.The Cochrane risk of bias method was used to assess the quality of included studies,and a Meta-analysis was conducted with Review Manager5.2 software.RESULTS:Eleven RCTs involving 1034 participants were included.The methodological quality was relatively passable.The Meta-analysis indicated that the combined use of DCI and conventional treatment with Western Medicine(WM)was more efficacious in the outcomes of total effective rate[Relative Risk(RR)=1.27,95%CI(confidence interval;1.18,1.35),P<0.000 01],the total effective rate of ECG[RR=1.40,95%CI(1.18,1.66),P<0.000 01],total cholesterol[Mean difference(MD)=-0.58,95%CI(-0.83,-0.33),P<0.000 01],total triglycerides[MD=-0.36,95%CI(-0.54,-0.17),P=0.0001],and the number of ST-segment depression[MD=-0.36,95%CI(-0.54,-0.17),P=0.0001].There were two adverse drug reactions reported in one study.CONCLUSION:Based on the systematic review,DCI combined with WM appeared to be efficacious in the treatment UAP.However,the evidence of DCI for treating UAP requires large-scale and double-blind RCTs to substantiate these findings.
