Professor Heinz Konietzky is a graduate （Dipl.-Ing. 1987; Dr.-Ing. 1989;Dr.-Ing. habil. 2001） of the TU Bergakademie Freiberg in the field ofGeotechnical Engineering.
Currently,the BeiDou⁃3(BDS⁃3)precise point positioning(PPP)service(PPP⁃B2b)mostly employs the ionosphere⁃free(IF)combination model for precise timing,which tends to amplify the noise in observation values.To address t...Currently,the BeiDou⁃3(BDS⁃3)precise point positioning(PPP)service(PPP⁃B2b)mostly employs the ionosphere⁃free(IF)combination model for precise timing,which tends to amplify the noise in observation values.To address this issue,this paper proposes a real⁃time BDS⁃3 precise unidirectional timing model based on uncombined(UC)observations using the BDS⁃3 PPP⁃B2b service.This model resolves the challenge of the amplified observation noise inherent in the IF combination model.The experiment involved selecting eight global navigation satellite system(GNSS)observation stations within China and collecting continuous observation data for 15 d.A comparative analy⁃sis with the traditional dual⁃frequency IF combination PPP timing model showed that the BDS⁃3 UC PPP timing based on the BDS⁃3 PPP⁃B2b service can achieve a timing preci⁃sion of 0.5 ns.In addition,it was found that due to global positioning system(GPS)satellite clock products in the BDS⁃3 PPP⁃B2b service not being unified to the standard time,the GPS IF PPP timing method based on the BDS⁃3 PPP⁃B2b service is not recommended for precise timing.In summary,the BDS⁃3 UC PPP timing model proposed in this paper is suitable for precise timing,providing observa⁃tion values with smaller noise,and its timing accuracy is comparable to that of the BDS⁃3 IF PPP,with slightly better frequency stability.展开更多
Understanding migration patterns and spatial connectivity is crucial for conserving long-distance migratory birds. While satellite telemetry has advanced the study of large gulls, Pallas's Gull (Ichthyaetus ichthy...Understanding migration patterns and spatial connectivity is crucial for conserving long-distance migratory birds. While satellite telemetry has advanced the study of large gulls, Pallas's Gull (Ichthyaetus ichthyaetus) remains relatively understudied, with limited data on its migration routes and habitat use, particularly in Central Asia. This study integrates 684 ring recoveries (1968–2024) and GPS tracking data to analyze the migration ecology of individuals breeding at Alakol Lake, Kazakhstan. Ring recoveries confirm migratory connectivity across Kazakhstan, Russia, Uzbekistan, Turkmenistan, Iran, and Pakistan, with wintering records as far as India, Kuwait, Bangladesh, and Ethiopia. GPS tracking of a single individual (June 2020–August 2021) revealed a migration route from Alakol Lake to the Arabian Sea, with key stopovers at Zaisan Lake, Balkhash Lake, the Aral Sea, Aydar Lake, and the Amu Darya River. Notably, a post-breeding northward dispersal to Zaisan Lake and southern Russia was identified before the southward migration commenced. These findings highlight the significance of Kazakhstan's lakes as breeding and migratory hubs and the need to protect critical stopover sites in Central Asia. Given increasing anthropogenic pressures on wetland habitats, this research provides essential baseline data for conservation planning and enhances the broader understanding of gull migration ecology.展开更多
目的:研究肿瘤抑制基因人ING4(inhibitor of growth family,member4)对C6鼠胶质瘤细胞的促凋亡作用。方法:将携有绿色荧光蛋白(GFP)腺病毒空载体Ad及重组腺病毒Ad-hING4-His(由本科室构建)分别感染C6细胞,RT-PCR法检测hING4的转录,Weste...目的:研究肿瘤抑制基因人ING4(inhibitor of growth family,member4)对C6鼠胶质瘤细胞的促凋亡作用。方法:将携有绿色荧光蛋白(GFP)腺病毒空载体Ad及重组腺病毒Ad-hING4-His(由本科室构建)分别感染C6细胞,RT-PCR法检测hING4的转录,Western-blotting法检测目的蛋白的表达。并观测hING4基因表达对C6胶质瘤细胞的作用,用MTT法绘制生长曲线,计算抑瘤率。再取重组腺病毒Ad-hING4-His及空腺病毒Ad作用后的C6细胞分别行激光共聚焦显微镜观察凋亡小体、透射电镜观察亚细胞结构的变化,抽提基因组DNA行琼脂糖凝胶电泳及流式细胞仪检测。结果:Ad-hING4-His感染C6细胞后,RT-PCR及Western-blotting结果提示有目的基因的转录和表达。hING4基因表达可以显著抑制C6细胞生长。激光共聚焦观察可见明显核断裂、透射电镜可见实验组细胞呈凋亡表现、基因组DNA电泳呈现梯形条带,流式细胞仪检测有明显AP峰,凋亡率达18.1%。结论:hING4可以通过促进细胞凋亡作用而显著抑制C6细胞的增殖和生长。展开更多
文摘Professor Heinz Konietzky is a graduate （Dipl.-Ing. 1987; Dr.-Ing. 1989;Dr.-Ing. habil. 2001） of the TU Bergakademie Freiberg in the field ofGeotechnical Engineering.
基金The Basic Science Center Project of the National Natural Science Foundation of China(No.42388102)the Jiangsu Province Natural Resources Science and Technology Project(No.JSZRKJ202404).
文摘Currently,the BeiDou⁃3(BDS⁃3)precise point positioning(PPP)service(PPP⁃B2b)mostly employs the ionosphere⁃free(IF)combination model for precise timing,which tends to amplify the noise in observation values.To address this issue,this paper proposes a real⁃time BDS⁃3 precise unidirectional timing model based on uncombined(UC)observations using the BDS⁃3 PPP⁃B2b service.This model resolves the challenge of the amplified observation noise inherent in the IF combination model.The experiment involved selecting eight global navigation satellite system(GNSS)observation stations within China and collecting continuous observation data for 15 d.A comparative analy⁃sis with the traditional dual⁃frequency IF combination PPP timing model showed that the BDS⁃3 UC PPP timing based on the BDS⁃3 PPP⁃B2b service can achieve a timing preci⁃sion of 0.5 ns.In addition,it was found that due to global positioning system(GPS)satellite clock products in the BDS⁃3 PPP⁃B2b service not being unified to the standard time,the GPS IF PPP timing method based on the BDS⁃3 PPP⁃B2b service is not recommended for precise timing.In summary,the BDS⁃3 UC PPP timing model proposed in this paper is suitable for precise timing,providing observa⁃tion values with smaller noise,and its timing accuracy is comparable to that of the BDS⁃3 IF PPP,with slightly better frequency stability.
基金funded by the Ministry of Science and Higher Education of the Republic of Kazakhstan,the Scientific Program BR21882199–Cadastre of wild animals of arid territories of the Balkhash-Alakol basin with an assessment of threats for their conservation and sustainable use.
文摘Understanding migration patterns and spatial connectivity is crucial for conserving long-distance migratory birds. While satellite telemetry has advanced the study of large gulls, Pallas's Gull (Ichthyaetus ichthyaetus) remains relatively understudied, with limited data on its migration routes and habitat use, particularly in Central Asia. This study integrates 684 ring recoveries (1968–2024) and GPS tracking data to analyze the migration ecology of individuals breeding at Alakol Lake, Kazakhstan. Ring recoveries confirm migratory connectivity across Kazakhstan, Russia, Uzbekistan, Turkmenistan, Iran, and Pakistan, with wintering records as far as India, Kuwait, Bangladesh, and Ethiopia. GPS tracking of a single individual (June 2020–August 2021) revealed a migration route from Alakol Lake to the Arabian Sea, with key stopovers at Zaisan Lake, Balkhash Lake, the Aral Sea, Aydar Lake, and the Amu Darya River. Notably, a post-breeding northward dispersal to Zaisan Lake and southern Russia was identified before the southward migration commenced. These findings highlight the significance of Kazakhstan's lakes as breeding and migratory hubs and the need to protect critical stopover sites in Central Asia. Given increasing anthropogenic pressures on wetland habitats, this research provides essential baseline data for conservation planning and enhances the broader understanding of gull migration ecology.
文摘目的:研究肿瘤抑制基因人ING4(inhibitor of growth family,member4)对C6鼠胶质瘤细胞的促凋亡作用。方法:将携有绿色荧光蛋白(GFP)腺病毒空载体Ad及重组腺病毒Ad-hING4-His(由本科室构建)分别感染C6细胞,RT-PCR法检测hING4的转录,Western-blotting法检测目的蛋白的表达。并观测hING4基因表达对C6胶质瘤细胞的作用,用MTT法绘制生长曲线,计算抑瘤率。再取重组腺病毒Ad-hING4-His及空腺病毒Ad作用后的C6细胞分别行激光共聚焦显微镜观察凋亡小体、透射电镜观察亚细胞结构的变化,抽提基因组DNA行琼脂糖凝胶电泳及流式细胞仪检测。结果:Ad-hING4-His感染C6细胞后,RT-PCR及Western-blotting结果提示有目的基因的转录和表达。hING4基因表达可以显著抑制C6细胞生长。激光共聚焦观察可见明显核断裂、透射电镜可见实验组细胞呈凋亡表现、基因组DNA电泳呈现梯形条带,流式细胞仪检测有明显AP峰,凋亡率达18.1%。结论:hING4可以通过促进细胞凋亡作用而显著抑制C6细胞的增殖和生长。
